{"title":"Other ELISA Kit","description":null,"products":[{"product_id":"valproic-acid","title":"Sodium valproate ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a competitive ELISA method. The microplate is coated with valproate conjugated antigen, and the sodium valproate standard or sample is added. Free sodium valproate and the sodium valproate conjugated antigen pre coated on the microplate compete with each other for the anti valproate antibody enzyme marker, which is developed with TMB substrate. The color changes from blue to yellow after adding the stop solution, The absorbance value is inversely proportional to the content of sodium valproate in the sample, and the content of sodium valproate in the sample is calculated by the standard curve.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eKit Composition: \u003c\/strong\u003e\u003cbr\u003e1. Pre coated sodium valproate conjugated antigen detachable enzyme plate: 1 piece (12 well × 8 pieces)\u003cbr\u003e2. Sodium valproate standard: 6 bottles (1ml\/ bottle), the contents are: 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb\u003cbr\u003e3. Anti sodium valproate antibody enzyme conjugate: 1 bottle (6ml)\u003cbr\u003e4. Chromogenic solution a:1 bottle (6ml)\u003cbr\u003e5. Chromogenic solution B: 1 bottle (6ml)\u003cbr\u003e6. Termination solution: 1 bottle (6ml), 2m sulfuric acid\u003cbr\u003e7. Sample diluent: 1 bottle (10×, 6ml) for sample dilution\u003cbr\u003e8. Concentrated washing solution: 1 bottle (20×, 20ml) for plate washing\u003cbr\u003e9. One copy of instructions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please read the instructions carefully before using the reagent kit\u003cbr\u003e2. Do not use expired reagent kits\u003cbr\u003e3. Before using the reagent kit, restore the reagent to room temperature (25± 2 ℃), and it is recommended to warm it up for at least 2 hours\u003cbr\u003e4. The standard product contains sodium valproate, and special attention should be paid when using it. Gloves should be worn during operation\u003cbr\u003e5. The termination solution contains sulfuric acid, which prevents skin burns and corrosion of clothing when used\u003cbr\u003e6. The suction heads used for different standards and samples cannot be mixed, otherwise it will affect the test results\u003cbr\u003e7. Reagents from different batch number kits should not be mixed; The suction heads used for different standards and samples should not be mixed, otherwise it will affect the experimental results\u003cbr\u003e8. When diluting the sample, the sample diluent in this reagent kit must be used, otherwise it will affect the experimental results\u003cbr\u003e9. Avoid foaming when mixing reagents\u003cbr\u003e10. Samples tested positive in this kit should be confirmed using another method such as HPLC or GC\/MS.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. The kit should be stored at 2-8 ℃ and should not be frozen. \u003cbr\u003e2. Unused microplates should be sealed and stored dry.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe standard curve ranged from 0.1ppb to 8.1ppb.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518169915467,"sku":"abs553809-96T","price":598.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-mouse-rat-pge2-competitive-elisa-kit","title":"Human\/Mouse\/Rat PGE2 Competitive ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\/Mouse\/Rat Prostaglandin E2 Competitive ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses solid-phase competitive enzyme-linked immunosorbent assay technology. Rabbit anti mouse antibody was pre coated on a high affinity microplate. PGE2 specific monoclonal antibody was added to the wells, incubated, and bound to the solid-phase antibody. After washing, biotin labeled PGE2 and unlabeled PGE2 or samples were added to compete for binding to the limited binding sites on the monoclonal antibody. After washing to remove unbound material, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, the chromogenic substrate was added, and the depth of color reaction was inversely proportional to the concentration of PGE2. The reaction was stopped by adding a stop solution, and the absorbance value was measured at a wavelength of 450nm (reference wavelength 570-630nm). This kit has validated human, mouse and rat serum samples, but PGE2 of other mammals is also expected to be detected.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eSolid phase competition method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: A\u003c\/strong\u003e copy of pre coated 96 well plate, standard, PGE2 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.08pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e92-102%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231026\/0c7a768f438d4d1fa7deab4c59566dad.png\" alt=\"\" width=\"410\" height=\"246\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProstaglandin E2 (PGE2), also known as dinoprostone, is a natural prostaglandin that can be used as a drug. PGE2 is a drug of the oxytocin family. It binds and activates prostaglandin E2 receptors, which in turn leads to the opening and softening of the cervix and the expansion of blood vessels. It is a direct vasodilator that relaxes smooth muscle and inhibits the release of norepinephrine from sympathetic nerve terminals. PGE2 also plays a role in hippocampal synaptic plasticity and febrile response. In addition, pge2  It also stimulates tumor cell proliferation and differentiation and tumor associated neovascularization. PGE2 also inhibits T cell receptor signaling and may play a role in the resolution of inflammation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518171422795,"sku":"abs554101-96T","price":600.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cat-ifn-a-elisa-kit","title":"Cat IFN-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250603\/d308397d4bf84a8e89449fe3f1954677.png\" alt=\"\" width=\"600\" height=\"317\"\u003e\u003cbr\u003e\u003cp\u003eThis figure is for reference only.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable concentration was less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Cat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, and HRP-labeled detection antibody are added sequentially to microwells pre-coated with feline interferon-α (IFN-α) capture antibody. The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of feline interferon-α (IFN-α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFeline IFN-α ELISA Kit; Feline IFN-alpha ELISA Kit; Feline IFNα ELISA Kit; Feline IFN alpha ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of feline interferon-α (IFN-α) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standards are: 800, 400, 200, 100, 50, and 25 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 pg\/mL – 800 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678414151755,"sku":"abs554470-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"asd-elisa-kit","title":"ASD ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003eSerum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003ePlasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eCell Supernatant: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse the tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePreparation before testing:\u003c\/strong\u003e\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng\/mL). Then dilute to the following concentrations: 10 ng\/mL, 5 ng\/mL, 2.5 ng\/mL, 1.25 ng\/mL, 0.625 ng\/mL, 0.3125 ng\/mL, 0.15625 ng\/mL, and 0 ng\/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng\/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate from the penultimate tube. See the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotin-Antibody Working Solution: 15 minutes before use, centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day.\u003cbr\u003e4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day.\u003cbr\u003e5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.)\u003cbr\u003e3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes.\u003cbr\u003e5. Wash: Discard the liquid and wash the plate five times as in step 3.\u003cbr\u003e6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003eCalculation of experimental results:\u003cbr\u003eResult evaluation:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with the universal species Androstenedione (ASD) antigen. After incubation and washing, the assay is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of universal species Androstenedione (ASD) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAndrostenedione ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompetition Law\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate  Biotin- Antibody ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e60uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAndrostenedione, or 4-androstenedione (abbreviated as A4 or Δ4-dione), also known as androsten-4-ene-3,17-dione, is an endogenous weak androgenic steroid hormone and an intermediate in the biosynthesis of estrone and testosterone from dehydroepiandrosterone (DHEA). It is closely related to androstenediol (androst-5-ene-3β,17β-diol). It is a precursor of testosterone and other androgens, as well as a precursor of estrogens such as estrone. In addition to functioning as an endogenous prohormone, androstenedione itself possesses weak androgenic activity. Androstenedione has been found to have some estrogenic activity, similar to other DHEA metabolites.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.15-10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, cell supernatant, tissue homogenate, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678418706507,"sku":"abs554139-96T","price":368.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rabbit-il-1-elisa-kit","title":"Rabbit IL-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003eSerum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003ePlasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003eTissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e\u003cbr\u003eCell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect them by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis.\u003cbr\u003e\u003cbr\u003eCell culture supernatant: Centrifuge at 1000×g for 20 minutes, remove the supernatant, or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003cbr\u003eOther biological fluids: Centrifuge at 1000×g for 20 minutes, and remove the supernatant for analysis.\u003cbr\u003e\u003cbr\u003ePre-Assay Preparation:\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e\u003cbr\u003e2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000 pg\/mL). Then dilute to the following concentrations: 1000 pg\/mL, 500 pg\/mL, 250 pg\/mL, 125 pg\/mL, 62.5 pg\/mL, 31.25 pg\/mL, 15.625 pg\/mL, and 0 pg\/mL.\u003cbr\u003e\u003cbr\u003eSerial Dilution Method: Add 500 μL of universal diluent to each of seven EP tubes. Pipette 500 μL of the 1000 pg\/mL standard working solution into the first EP tube and mix thoroughly to make a 500 pg\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use.\u003cbr\u003e\u003cbr\u003e4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately.\u003cbr\u003e\u003cbr\u003e5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C.\u003cbr\u003e\u003cbr\u003e2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.)\u003cbr\u003e\u003cbr\u003e3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes.\u003cbr\u003e\u003cbr\u003e4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e\u003cbr\u003e5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes.\u003cbr\u003e\u003cbr\u003e6. Washing: Discard the liquid and wash the plate five times as in step 4.\u003cbr\u003e\u003cbr\u003e7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e\u003cbr\u003e8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003eCalculating experimental results:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/b6a4e2d01b794c3ebc3ece3730a604f4.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 1 (IL-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 1 (IL-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRabbit Interleukin 1 ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Concentrated biotinylated antibody ( 100× ）  \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678469103691,"sku":"abs554140-96T","price":368.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-ctnt-elisa-kit","title":"Monkey cTnT ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ecTnT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\n\u003cstrong\u003e 1. \u003c\/strong\u003e\u003cstrong\u003e Sample handling and requirements \u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cstrong\u003e Serum: \u003c\/strong\u003e\u003cbr\u003eWhole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4℃ Overnight, then 1000×g Centrifugation 20 Minutes, take the supernatant, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cstrong\u003e Plasma: \u003c\/strong\u003e\u003cbr\u003euse EDTA Or heparin as an anticoagulant to collect specimens, and collect the specimens after collection 30 Within minutes 2-8℃ 1000\u003c\/p\u003e\n\u003cp\u003e×g Centrifugation 15 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cstrong\u003e Tissue homogenate: \u003c\/strong\u003e\u003cbr\u003eWith pre-cooled PBS (0.01M, pH=7.4) The tissue is flushed to remove residual blood (lysed red blood cells in the homogenate can affect the measurement), and the tissue is weighed and cut into pieces. Combining the shredded tissue with the corresponding volume PBS (Generally according to 1:9 Weight to volume ratio, such as 1g The tissue samples correspond to 9mL Of PBS The specific volume can be appropriately adjusted according to the experimental needs and recorded. Recommended in PBS Add protease inhibitor) into a glass homogenizer and ground thoroughly on ice. For further lysis of tissue cells, the homogenate can be sonicated, or freeze-thawed repeatedly. Finally, the homogenate was mixed in 5000×g Centrifugation 5~10 Minutes,   Take the supernatant for detection. \u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003e Cell culture supernatant or other biological specimen: \u003c\/strong\u003e\u003cbr\u003ePlease 1000×g Centrifugation 20 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e Note: Hemolysis of the specimen will affect the final test result, so hemolyzed specimens should not be tested. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003e Two, \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation \u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e The kit should be removed from the refrigerated environment and equilibrated at room temperature before use. \u003c\/p\u003e\n\u003cp\u003e20× Dilution of wash buffer: distilled water according to 1 ： 20 Dilution, i.e. 1 Share 20× Wash buffer plus 19 Distilled water. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003e Three, \u003c\/strong\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e1. Equilibration from room temperature  20min  Take out the required slats from the aluminum foil bag after that, and seal the remaining slats with ziplock bag and put them back  4℃ 。 \u003c\/p\u003e\n\u003cp\u003e2. Set up standard wells and sample wells, add different concentrations of standards to each standard well  50μL ； \u003c\/p\u003e\n\u003cp\u003e3. The sample to be tested is added to the sample well  50μL ； Blank holes are not added. \u003c\/p\u003e\n\u003cp\u003e4. In addition to the blank wells, horseradish peroxidase ( HRP ) Labeled detection antibody \u003c\/p\u003e\n\u003cp\u003e100μL Sealing the reaction hole with a sealing plate membrane, 37℃ Incubation in water bath or incubator  60min 。 \u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid, pat dry on absorbent paper, and fill each well with wash liquid ( 350μL ), let stand  1min , throw off the washing liquid, pat dry on absorbent paper, and repeat washing the plate  5  Times (the plate can also be washed with a plate washing machine). \u003c\/p\u003e\n\u003cp\u003e6. Substrate added per well  A 、 B  Each  50μL ， 37℃ Incubate in the dark  15min 。 \u003c\/p\u003e\n\u003cp\u003e7. Add stop solution to each well  50μL ， 15min  Inside, in  450nm  Wavelength measurement of each well  OD  Value. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e Calculation of experimental results \u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003e Based on the tested standard OD The value is the abscissa, and the concentration value of the standard product is the ordinate. Draw the standard curve on coordinate paper or with relevant software, and obtain the linear regression equation. The sample OD Values are substituted into Eq., and the concentration of the sample is calculated. \u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250205\/55f279a3f4684886b17870e9faa6789e.png\" alt=\"\" width=\"716\" height=\"422\"\u003e\u003cbr\u003e Standard curve \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e Note: Repeatability: Intra-plate coefficient of variation is less than  10%  , the interplate coefficient of variation is less than  15%  。 \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). The specimen, standard and HRP-labeled detection antibody were sequentially added to the coated microwells pre-coated with monkey myocardial-specific troponin T (cTnT) capture antibody, incubated and thoroughly washed. The color is developed with the substrate TMB, which is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a positive correlation between the depth of color and monkey cardiac muscle-specific troponin T (cTnT) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is used to quantitatively detect the content of monkey myocardial specific troponin T (cTnT) in serum, plasma, tissue homogenate and related liquid samples in vitro.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; height: 709px; width: 54.9074%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Microwell microplate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e12  Hole ×8  Strip \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e0.3mL*6  Tube \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Detection antibodies -HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e20× Wash buffer \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e Dilute as per instructions \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Substrate  A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Substrate  B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.9901%;\"\u003e\u003cp\u003e Stop Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%;\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.9901%; text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4111%; text-align: center;\"\u003e\u003cp\u003e2  Sheets \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.5671%;\"\u003e\u003cp style=\"text-align: center;\"\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e remark \u003c\/strong\u003e ： \u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e The standard concentrations are as follows: 1000 、 500 、 250 、 125 、 62.5 、 31.25pg\/mL\u003c\/li\u003e\n\u003cli\u003e  After inspection of a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit, which are directly taken during the experiment 50μL Just load the sample. When some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before conducting the experiment. \u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Incubate in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Store reagents in refrigeration immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Incorrect plate washing can lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the wells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Eliminate the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate color development solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to avoid wrong results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. After equilibrating to room temperature, open the sealed bag to prevent water droplets from condensing on the cold slats.\u003c\/p\u003e\n\u003cp\u003e8. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reaction reagents in the kit.\u003c\/p\u003e\n\u003cp\u003e9. Expired products cannot be used.\u003c\/p\u003e\n\u003cp\u003e10. If it is possible to spread diseases, all samples should be managed, and the samples and testing devices should be handled according to the prescribed procedures.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.25 pg\/mL-1000 pg\/mL; The lowest detected concentration was less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678469365835,"sku":"abs554115-96T","price":444.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-kim-1-elisa-kit","title":"Monkey Kim-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eI.\u003c\/strong\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eII.\u003c\/strong\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eIII.\u003c\/strong\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cstrong\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003c\/strong\u003e\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cstrong\u003eSet up standard wells and sample wells.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cstrong\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003c\/strong\u003e\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cstrong\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with 350 μL of wash buffer.\u003c\/strong\u003e\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a microplate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cstrong\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cstrong\u003eAdd 50 μL of stop solution to each well.\u003c\/strong\u003e\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eCalculation of Experimental Results: Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250210\/68bff2b504f6466fa2214784db12f60c.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eStandard Curve\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eNote:\u003cbr\u003eSpecificity: No cross-reaction with other soluble structural analogs.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eReproducibility: Intra-plate coefficient of variation is less than 10%, and inter-plate coefficient of variation is less than 15%.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a capture antibody against monkey kidney injury molecule 1 (Kim-1). The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey kidney injury molecule 1 (Kim-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey HAVCR1 ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of the content of monkey kidney injury molecule 1 (Kim-1) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 50%; height: 540px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003e96\u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eMicrowell enzyme plate\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e12 holes×8 strips\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eStandard product\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e0.3mL*6 tube\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eSample diluent\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e6mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eDetection antibody-HRP\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e10mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e20× Wash Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e25mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eDilution according to the instructions\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eSubstrate A\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e6mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eSubstrate B\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e6mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"height: 54px; width: 36.6183%;\" width=\"260\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eStop solution\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.7321%;\" width=\"204\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e6mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e2 pictures\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 28.8732%;\" width=\"205\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNone\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr notes: the concentrations of standard solutions are: and pg after testing a large number normal specimens are all within detection range provided by kit. during experiment sample can be directly loaded. if some values exceed maximum concentration appropriately diluted with diluent before experiment.\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 4°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 pg\/mL – 160 pg\/mL; Sensitivity: Minimum detection concentration is less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678470283339,"sku":"abs554116-96T","price":476.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-cfb-elisa-kit","title":"Monkey CFB ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCFB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e 1. \u003c\/strong\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e2. Plasma: the sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in       -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment. \u003c\/p\u003e\n\u003cp\u003e3. If the concentration of the test substance in your sample is higher than the highest value of the standard substance, please dilute it according to the actual situation (it is recommended to do it first    Pre-experiments to determine the dilution factor). \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Two, \u003c\/strong\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 20 µg\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 20 µg\/mL ， 10 µg\/mL ， 5 µg\/mL ， 2.5 µg\/mL ， 1.25 µg\/mL ， 0.63 µg\/mL ， 0.32 µg\/mL Standard dilution ( 0 µg\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250210\/b75545bd9d0d46e98724863b0d602d98.png\" alt=\"\" width=\"800\" height=\"311\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette. \u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette. \u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before use of the kit.\u003cbr\u003eIf the reconstituted standard is not used, please discard it. \u003c\/p\u003e\n\u003cp\u003e2. Concentrated biotinylated antibody, the volume of concentrated enzyme conjugate is small, may be dispersed in various parts of the tube during transportation, please 1000×g Centrifugation 1 Minutes to allow the liquid of the tube wall or cap to deposit to the bottom of the tube.\u003cbr\u003ePipette carefully before use 4-5 The solution was mixed once.\u003cbr\u003eStandard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be use without confusion. \u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon.\u003cbr\u003eThe crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40°C ）。\u003cbr\u003eThe wash liquid should be at room temperature when used. \u003c\/p\u003e\n\u003cp\u003e4. Adding samples should be quick, and it is best to control each sample adding within 10 Within minutes, in order to ensure the accuracy of the experiment, it is recommended to use a double hole.\u003cbr\u003eWhen pipetting reagents, a consistent sequence of addition is maintained from well to well,\u003cbr\u003eThis will ensure the same hatch time for all holes. \u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper, and do not put the filter paper directly into the reaction hole to absorb water.\u003cbr\u003eBefore reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader. \u003c\/p\u003e\n\u003cp\u003e6. Color developer TMB Direct exposure to bright light should be avoided during storage and use.\u003cbr\u003eAfter adding the substrate, pay attention to the color change in the reaction well.\u003cbr\u003eIf the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader. \u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used in the experiment are disposable, and it is strictly forbidden to reuse them, otherwise it will affect the experimental results. \u003c\/p\u003e\n\u003cp\u003e8. During the experiment, please wear a laboratory coat and latex gloves for protection, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. \u003c\/p\u003e\n\u003cp\u003e9. The kit components of different lot numbers cannot be mixed (except wash solution and reaction stop solution). \u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strips in the kit are detachable plates, please use them in batches according to the experimental requirements. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Three, \u003c\/strong\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。 \u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto; height: 150px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration \u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003e\u003cstrong\u003eµg\/mL\u003c\/strong\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%; text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%; text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e2.154\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e2.059\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e10\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e1.896\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e1.801\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e1.276\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e1.181\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e2.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e0.958\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e0.863\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e1.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e0.617\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e0.522\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 极4974%;\" width=\"354\"\u003e\u003cp\u003e0.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e0.172\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e0.077\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.4974%;\" width=\"354\"\u003e\u003cp\u003e0.32\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%;\" width=\"240\"\u003e\u003cp\u003e0.226\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp\u003e0.131\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.4974%; text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.008%; text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.095\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.5027%;\" width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250210\/234d28d085a14e31b64efcd786426565.png\" alt=\"\" width=\"600\" height=\"337\"\u003e\u003cbr\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  CFB , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto; height: 150px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 27.8094%;\" width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5156%; text-align: center;\" width=\"350\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.7699%; text-align: center;\" width=\"355\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 27.8094%;\" width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5156%;\" width=\"350\"\u003e\u003cp\u003e87-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.7699%;\" width=\"355\"\u003e\u003cp\u003e93%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 27.8094%;\" width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5156%;\" width=\"350\"\u003e\u003cp\u003e92-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.7699%;\" width=\"355\"\u003e\u003cp\u003e98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 27.8094%; text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5156%; text-align: center;\" width=\"350\"\u003e\u003cp\u003e83-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.7699%;\" width=\"355\"\u003e\u003cp style=\"text-align: center;\"\u003e89%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e There will be monkeys added  CFB The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto; height: 150px; width: 68%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25.5617%;\" width=\"254\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.3394%;\" width=\"164\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 25.5617%;\" width=\"254\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e85-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e79-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e89-100%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3394%;\" width=\"164\"\u003e\u003cp\u003e85-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 25.5617%;\" width=\"254\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e87-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.5707%;\" width=\"极64\"\u003e\u003cp\u003e82-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3394%;\" width=\"164\"\u003e\u003cp\u003e78-90%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 25.5617%;\" width=\"254\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e78-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e83-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.5707%;\" width=\"164\"\u003e\u003cp\u003e79-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3394%;\" width=\"164\"\u003e\u003cp style=\"text-align: center;\"\u003e88-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey CFB antibody was coated in a 96-well microplate, and the monkey CFB standard or sample was added to the microwells respectively, so that the monkey CFB protein in the standard or the monkey CFB protein in the sample was bound to the anti-monkey CFB antibody solid on the microplate, then biotinylated anti-monkey CFB antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey CFB protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey Complement Factor B ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant or native monkey CFB can be detected and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; height: 400px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.0792%;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.0792%; text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4067%; text-align: center;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.5169%;\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. If the entire kit is stored at-20 °C, please place the kit at 4 °C the night before the experiment.\u003c\/p\u003e\n\u003cp\u003e2. Salt precipitation will occur when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve.\u003c\/p\u003e\n\u003cp\u003e3. The newly opened wells of the enzyme labeled plate may contain a little water-like substance, which is a normal phenomenon and will not have any impact on the experimental results.\u003c\/p\u003e\n\u003cp\u003e4. This kit is intended for laboratory research and development use only, not for human or animal use.\u003c\/p\u003e\n\u003cp\u003e5. Reagents should be treated as hazardous substances and should be handled carefully and properly disposed of.\u003c\/p\u003e\n\u003cp\u003e6. Gloves, lab coats and protective glasses should always be worn to avoid skin and eye contact with terminating solution and TMB. In case of accidental contact, please wash thoroughly with water.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.32-20 µ g\/mL; Sensitivity: 0.12 µ g\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678471233611,"sku":"abs554117-96T","price":413.0,"currency_code":"USD","in_stock":true}]},{"product_id":"chicken-egf-elisa-kit","title":"Chicken EGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003e1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e4. Cell lysates Cell culture sizingOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePre-test preparation:\u003c\/strong\u003e\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 8000pg\/mL). Then dilute to the following concentrations: 8000pg\/mL, 4000pg\/mL, 2000pg\/mL, 1000pg\/mL, 500pg\/mL, 250pg\/mL, 125pg\/mL, and 0pg\/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 8000pg\/mL standard working solution into the first EP tube and mix thoroughly to make a 4000pg\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details.\u003cbr\u003e3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately.\u003cbr\u003e4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately.\u003cbr\u003e5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProcedure:\u003c\/strong\u003e\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C.\u003cbr\u003e2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.)\u003cbr\u003e3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes.\u003cbr\u003e4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes.\u003cbr\u003e6. Washing: Discard the liquid and wash the plate five times as in step 4.\u003cbr\u003e7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCalculating experimental results:\u003c\/strong\u003e\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/deda9c2471694d7b8a0c7e9f5d3048e5.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with an Epidermal Growth Factor (EGF) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Epidermal Growth Factor (EGF) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChicken\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChicken Epidermal Growth Factor ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Concentrated biotinylated antibody ( 100× ）  \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEpidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. It was originally described as a secreted peptide. By binding to its cognate receptor, it induces cell proliferation, differentiation, and survival. EGF can be found in urine, saliva, chicken milk, tears, and plasma. It is also found in the submandibular and parotid glands. EGF production has been shown to be stimulated by testosterone. Saliva EGF appears to be regulated by dietary inorganic iodine and also plays an important physiological role in maintaining the integrity of oroesophageal and gastric tissues. EGF exerts its effects by binding with high affinity to the epidermal growth factor receptor (EGFR) on the cell surface.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e125-8000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture slurries and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678471430219,"sku":"abs554141-96T","price":368.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-nfl-elisa-kit","title":"Monkey NFL ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eTissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eCell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003eReagent Preparation After removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cp\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003eProcedure 1.\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e2.\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e5.\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with 350 μL of wash buffer.\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a microplate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e6.\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e7.\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003eCalculation of Experimental Results: Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250318\/c54b529b4d1542ebabd779616640ef97.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Monkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with a capture antibody against monkey neurofilament light chain (NF-light). The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey neurofilament light chain (NF-light) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey NF-light ELISA Kit；Monkey NEFL ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of the content of monkey neurofilament light chain protein (NFL) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 80, 40, 20, 10, 5, and 2.5 ng\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, sealed and stored at 2-8℃, valid for 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDetection range: 2.5 ng\/mL – 80 ng\/mL; Sensitivity: Minimum detection concentration is less than 0.1 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678472314955,"sku":"abs554426-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-t-tau-elisa-kit","title":"Monkey T-TAU ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250227\/6c33f04145a24b3ebc96b73b9da4b555.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a capture antibody against monkey total tau protein (T-Tau). The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey total tau protein (T-Tau) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor in vitro quantitative detection of monkey total Tau protein (T-Tau) content in serum, plasma, tissue homogenate and related liquid samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 3200, 1600, 800, 400, 200, and 100 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 4°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 pg\/mL – 3200 pg\/mL; Sensitivity: Minimum detection concentration is less than 10 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678472446027,"sku":"abs554420-96T","price":397.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-hcg-elisa-kit","title":"Monkey HCG ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e\u003cbr\u003ePlace whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e\u003cbr\u003eCollect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e\u003cbr\u003eRinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e\u003cbr\u003eCentrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003cbr\u003eHemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250303\/7f9a84806edb45f394e6804d486e0ba5.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a human chorionic gonadotropin (hCG) capture antibody. The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of human chorionic gonadotropin (hCG) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey chorionic gonadotropin (HCG) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 8, 4, 2, 1, 0.5, and 0.25 mIU\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly loaded. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 4°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.25 mIU\/mL – 8 mIU\/mL; Sensitivity: minimum detection concentration is less than 0.1 mIU\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678473560139,"sku":"abs554421-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-anti-klh-igg-elisa-kit","title":"Monkey anti-KLH IgG ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eTissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eCell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003eReagent Preparation\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cp\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003eProcedure\u003cbr\u003e1.\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e2.\u003cbr\u003eSet up negative control wells, positive control wells, and sample wells.\u003cbr\u003eAdd 50 μL of control sample to each negative and positive control well, add 50 μL of the sample to be tested to the sample wells, and leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-conjugated detection antibody to each control and sample well, except for the blank wells.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e5.\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e6.\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1.\u003cbr\u003eNegative control OD value: less than 0.2.\u003c\/p\u003e\n\u003cp\u003e2.\u003cbr\u003ePositive control OD value: greater than 0.8.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003ePositive determination (Cut-Off value): If the negative control OD value is +0.25 and the sample OD value is greater than the threshold, it is considered positive; otherwise, it is negative.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eRepeatability: The intra-plate coefficient of variation is less than 15%.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses an indirect enzyme-linked immunosorbent assay (ELISA). Samples, negative and positive controls are added sequentially to microwells pre-coated with monkey anti-hemocyanin IgG (anti-KLH IgG) to capture the antigen. HRP-labeled detection antibodies are then added. After incubation and thorough washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of peroxidase and to a final yellow color under the action of acid. The intensity of the color is positively correlated with the presence of monkey anti-hemocyanin IgG (anti-KLH IgG) in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to determine positive or negative results.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro qualitative detection of monkey anti-hemocyanin IgG antibodies (anti-KLH IgG) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto; height: 752px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e96 wells\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eNegative control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e0.3mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003ePositive control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e0.3mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eDetection of antibodies-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003e20× Wash Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e2 photos\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore in a sealed container at 2-8℃ away from light, with a shelf life of 6 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678474510411,"sku":"abs554423-96T","price":413.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-klh-elisa-kit","title":"Monkey KLH ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px;\"\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight.\u003cbr\u003eThen, centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cp\u003eDilution of 20× Wash Buffer: Dilute the sample 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px;\"\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up negative control wells, positive control wells, and sample wells.\u003cbr\u003eAdd 50 μL of the control substance to each negative and positive control well.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; do not add any to the blank wells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each control well and sample well, except for the blank well.\u003cbr\u003eSeal the wells with plate sealing film and incubate at 37°C in a water bath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash solution (350 μL).\u003cbr\u003eLet stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003eCalculation of Experimental Results\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eNegative control OD value: less than 0.2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003ePositive control OD value: greater than 0.8.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003ePositive judgment (Cut-Off value): If the negative control OD value is +0.25 and the sample OD value is greater than the threshold, it is judged as positive, otherwise it is negative.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Samples, negative and positive controls, and HRP-labeled detection antibodies are added sequentially to wells pre-coated with a capture antibody against keyhole limpet hemocyanin (KLH). The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of keyhole limpet hemocyanin (KLH) in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to determine positive or negative results.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Used for in vitro qualitative detection of monkey keyhole limpet hemocyanin (KLH) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96\u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell ELISA Plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e96 Wells\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eNegative Control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003ePositive Control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample Diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection Antibody - HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× Wash Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 photos\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened test kit is sealed and stored at 2-8°C, it is valid for 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678475591755,"sku":"abs554424-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-gfap-elisa-kit","title":"Monkey GFAP ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eTissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eCell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003eReagent Preparation\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003eProcedure\u003cbr\u003e1.\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e2.\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e5.\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e6.\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e7.\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003eCalculation of Experimental Results:\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250313\/b91a6eb68cf24a63a1446ea8c86a2b1c.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with a monkey glial fibrillary acidic protein (GFAP) capture antibody. The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the monkey glial fibrillary acidic protein (GFAP) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of the content of monkey glial fibrillary acidic protein (GFAP) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto; height: 752px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%; text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e12 holes×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e0.3mL*6 tube\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eDetection Antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003e20×Wash Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eDilute according to instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5741%;\" width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.5741%; text-align: center;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5519%; text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 Sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9826%;\" width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard substances are: 160, 80, 40, 20, 10, and 5 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, sealed and stored at 2-8℃, valid for 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 pg\/mL – 160 pg\/mL; Sensitivity: Minimum detection concentration is less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678476836939,"sku":"abs554425-96T","price":397.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-uchl1-elisa-kit","title":"Monkey UCHL1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Collection, Processing, and Storage Methods\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e1. Serum: Use pyrogen- and endotoxin-free tubes.\u003cbr\u003eAvoid any cell stimulation during the procedure.\u003cbr\u003eAfter blood collection, centrifuge at 3000 rpm for 10 minutes to quickly and carefully separate the serum from the red blood cells.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: Anticoagulate with EDTA, citrate, or heparin.\u003cbr\u003eCentrifuge at 3000 rpm for 30 minutes and remove the supernatant.\u003c\/p\u003e\n\u003cp\u003e3. Cell Supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and aggregates.\u003c\/p\u003e\n\u003cp\u003e4. Tissue Homogenate: Add an appropriate amount of saline to the tissue and mash.\u003cbr\u003eCentrifuge at 3000 rpm for 10 minutes and remove the supernatant.\u003c\/p\u003e\n\u003cp\u003e5. Storage: If samples are not tested promptly after collection, aliquot them into single-use aliquots and freeze at -20°C to avoid repeated freezing and thawing.\u003cbr\u003eThaw at room temperature and ensure the sample is evenly and fully thawed.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eDilution of 20× Wash Buffer: Dilute distilled water 1:20, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003ePlate Washing Method\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e1. Manual Plate Washing: Shake off all liquid in the wells, fill each well with wash buffer, let stand for 1 minute, shake off all liquid in the wells, and pat dry on absorbent paper.\u003cbr\u003eWash the plate 5 times in this manner.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003eAutomatic plate washer: Add 350 μL of wash solution to each well, soak for 1 minute, and wash the plate 5 times.\u003cbr\u003e\u003cspan style=\"font-size: 24px;\"\u003e\u003cstrong\u003e\u003cbr\u003eOperational Steps\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e2. Set up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of a standard of varying concentration to each standard well.\u003c\/p\u003e\n\u003cp\u003e3. First, add 10 μL of the sample to be tested to the sample well, followed by 40 μL of sample diluent.\u003cbr\u003eDo not add anything to the blank well.\u003cbr\u003e4. Add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 min.\u003cbr\u003e5. Discard the liquid, pat dry on absorbent paper, then fill each well with wash buffer.\u003cbr\u003eLet stand for 1 min, discard the wash buffer, and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a microplate washer can also be used).\u003cbr\u003e6. Add 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 min.\u003cbr\u003e7. Add 50 μL of stop solution to each well and measure the OD value of each well at 450 nm within 15 min.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 24px;\"\u003e\u003cstrong\u003eResult Evaluation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDraw a standard curve: In an Excel worksheet, use the standard concentration as the horizontal axis and the corresponding OD value as the vertical axis to draw a linear regression curve for the standard.\u003cbr\u003eCalculate the concentration of each sample according to the curve equation.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250318\/41eba5d0fffa49c8884654965f19f1c3.png\" alt=\"\" width=\"600\" height=\"594\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with ubiquitin carboxyl-terminal esterase L1 (UCHL1) antibodies. The assay is incubated and thoroughly washed. The substrate TMB is used for color development. TMB converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of ubiquitin carboxyl-terminal esterase L1 (UCHL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePGP 9.5；UCHL1；UCHL-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"193\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eNote: The concentrations of standards (S0-S5) are: 0, 50, 100, 200, 400, 800 ng\/mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Store the kit at 2-8°C and equilibrate at room temperature for 20 minutes before use. Crystallization may occur in the concentrated wash buffer after removal from the refrigerator. This is normal. Heat in a water bath to completely dissolve the crystals before use.\u003c\/p\u003e\n\u003cp\u003e2. Immediately return unused strips to the ziplock bag and seal (dry at low temperature) for storage.\u003c\/p\u003e3. The S0 standard, with a concentration of 0, can be considered a negative control or blank. When following the instructions, the sample has been diluted 5-fold; the final result, multiplied by 5, is the actual sample concentration.4. Strictly follow the incubation times, addition volumes, and order specified in the instructions.\u003cp\u003e5. Shake all liquid components thoroughly before use.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, sealed and stored at 2-8℃, valid for 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 10 - 800ng\/mL; Sensitivity: minimum detection concentration is less than 10ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678477754443,"sku":"abs554427-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-lpa-elisa-kit","title":"Monkey LPA ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLPA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample handling and requirements \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cstrong\u003e Serum: \u003c\/strong\u003e\u003cbr\u003e Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4℃ Overnight, then 1000×g Centrifugation 20 Minutes, take the supernatant, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cstrong\u003e Plasma: \u003c\/strong\u003e\u003cbr\u003e use EDTA Or heparin as an anticoagulant to collect specimens, and collect the specimens after collection 30 Within minutes 2-8℃ 1000×g Centrifugation 15 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cstrong\u003e Tissue homogenate: \u003c\/strong\u003e\u003cbr\u003e With pre-cooled PBS (0.01M, pH=7.4) The tissue is flushed to remove residual blood (lysed red blood cells in the homogenate can affect the measurement), and the tissue is weighed and cut into pieces. Combining the shredded tissue with the corresponding volume PBS (Generally according to 1:9 Weight to volume ratio, such as 1g The tissue samples correspond to 9mL Of PBS The specific volume can be appropriately adjusted according to the experimental needs and recorded. Recommended in PBS Add protease inhibitor) into a glass homogenizer and ground thoroughly on ice. For further lysis of tissue cells, the homogenate can be sonicated, or freeze-thawed repeatedly. Finally, the homogenate was mixed in 5000×g Centrifugation 5~10 Minutes, \u003cbr\u003e Take the supernatant for detection. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003e Cell culture supernatant or other biological specimen: \u003c\/strong\u003e\u003cbr\u003e Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e Note: Hemolysis of the specimen will affect the final test result, so hemolyzed specimens should not be tested. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Reagent Preparation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e The kit should be removed from the refrigerated environment and equilibrated at room temperature before use. \u003c\/p\u003e\n\u003cp\u003e20× Dilution of wash buffer: distilled water according to 1 ： 20 Dilution, i.e. 1 Share 20× Wash buffer plus 19 Distilled water. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Equilibration from room temperature 20min \u003cbr\u003e Take out the required slats from the aluminum foil bag after that, and seal the remaining slats with ziplock bag and put them back 4℃ 。 \u003c\/p\u003e\n\u003cp\u003e2. Set up standard wells and sample wells, add different concentrations of standards to each standard well 50μL ； \u003c\/p\u003e\n\u003cp\u003e3. The sample to be tested is added to the sample well 50μL ； \u003cbr\u003e Blank holes are not added. \u003c\/p\u003e\n\u003cp\u003e4. In addition to the blank wells, horseradish peroxidase ( HRP ) Labeled detection antibody 100μL \u003cbr\u003e Sealing the reaction hole with a sealing plate membrane, 37℃ Incubation in water bath or incubator 60min 。 \u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid, pat dry on absorbent paper, and fill each well with wash liquid ( 350μL ), let stand 1min , throw off the washing liquid, pat dry on absorbent paper, and repeat washing the plate 5 Times (the plate can also be washed with a plate washing machine). \u003c\/p\u003e\n\u003cp\u003e6. Substrate added per well A 、 B  Each 50μL ， 37℃ Incubate in the dark 15min 。 \u003c\/p\u003e\n\u003cp\u003e7. Add stop solution to each well 50μL ， 15min  Inside, in 450nm Wavelength measurement of each well OD  Value. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Calculation of experimental results \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Based on the tested standard OD The value is the abscissa, and the concentration value of the standard product is the ordinate. \u003cbr\u003e Draw the standard curve on coordinate paper or with relevant software, and obtain the linear regression equation. \u003cbr\u003e The sample OD Values are substituted into Eq., and the concentration of the sample is calculated. \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250327\/ef5e47d077f84268a43c479d59105e3f.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003e Standard curve \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMinimum detected concentration less than 10 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). The specimen, standard, and HRP-labeled detection antibody were sequentially added to the coated microwells pre-coated with monkey lipoprotein α (LPA) capture antibody, incubated and thoroughly washed. The color is developed with the substrate TMB, which is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a positive correlation between the depth of color and monkey lipoprotein alpha (LPA) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey Lipoprotein a ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is used for quantitative detection of monkey lipoprotein alpha (LPA) in serum, plasma, tissue homogenate and related liquid samples in vitro.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Microwell microplate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12  Hole ×8  Strip \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6  Tube \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Detection antibodies -HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× Wash buffer \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e Dilute as per instructions \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Substrate  A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Substrate  B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e Stop Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2  Sheets \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003e without \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e remark \u003c\/strong\u003e ： \u003c\/p\u003e\n\u003cp\u003e1. The standard concentrations are as follows: 2000 、 1000 、 500 、 250 、 125 、 62.5 pg\/mL\u003c\/p\u003e2. After inspection of a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit, which are directly taken during the experiment 50μL Just load the sample. When some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before conducting the experiment. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Incubate in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Store reagents in refrigeration immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Incorrect plate washing can lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the wells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Eliminate the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate color development solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to avoid wrong results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. After equilibrating to room temperature, open the sealed bag to prevent water droplets from condensing on the cold slats.\u003c\/p\u003e\n\u003cp\u003e8. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reaction reagents in the kit.\u003c\/p\u003e\n\u003cp\u003e9. Expired products cannot be used.\u003c\/p\u003e\n\u003cp\u003e10. If it is possible to spread diseases, all samples should be managed, and the samples and testing devices should be handled according to the prescribed procedures.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, sealed storage at 2-8 ℃, shelf life 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5 pg\/mL – 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678478770251,"sku":"abs554428-96T","price":397.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-apob100-elisa-kit","title":"Monkey APOB100 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250327\/271210790b064375a3d717ae63440449.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe lowest detectable concentration was less than 1.0 μg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a capture antibody against monkey apolipoprotein B100 (apo-B100). The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey apolipoprotein B100 (apo-B100) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey apo-B100 ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey apolipoprotein B100 (apo-B100) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 1200, 600, 300, 150, 75, and 37.5 μg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly loaded. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, sealed and stored at 2-8℃, valid for 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e37.5 μg\/mL – 1200 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678479687755,"sku":"abs554429-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-anti-peg-igg-elisa","title":"Monkey Anti-PEG IgG ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnti-PEG IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to relevant literature.\u003cbr\u003eExperiments should be carried out as soon as possible after extraction.\u003cbr\u003eIf the test cannot be carried out immediately, the specimen can be placed in -20℃ Store, but avoid repeated freezing and thawing\u003c\/p\u003e\n\n\u003cp\u003e2. Unable to detect containing NaN3 Samples, due to NaN3 Inhibition of horseradish peroxidase (HRP) activity.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eOperation steps\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e1. Dilution of standard substances: This kit provides one original standard substance, and users can dilute it in a small test tube according to the following chart Interpretation.\u003c\/p\u003e\n\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp style=\"text-align: center;\"\u003e2000pg\/ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"162\"\u003e\u003cp\u003e5 No. Standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"505\"\u003e\u003cp\u003e150μl Addition of the original standard 150μl Standard dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e1000pg\/ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e4 No. Standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl Of 5 No. Standard addition 150μl Standard dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e500pg\/ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e3 No. Standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl Of 4 No. Standard addition 150μl Standard dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e250pg\/ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e2 No. Standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl Of 3 No. Standard addition 150μl Standard dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"154\"\u003e\u003cp\u003e125pg\/ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"162\"\u003e\u003cp\u003e1 No. Standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp style=\"text-align: center;\"\u003e150μl Of 2 No. Standard addition 150μl Standard dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\n\u003cp\u003e2. Sample addition: Set up blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested respectively.\u003cbr\u003eAccurate spike of standard on enzyme-labeled coated plate 50μl, first add sample diluent to the sample well to be tested 40μl, then add the sample to be tested 10μl (The final dilution of sample was 5 Times).\u003cbr\u003eAdd the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well.\u003c\/p\u003e\n\n\u003cp\u003e3. Incubation: After plate sealing with plate sealing film 37℃ Incubation 30 Minutes.\u003c\/p\u003e\n\n\u003cp\u003e4. Solution preparation: will 30 Distilled water for double concentrated washing liquid 30 Time dilution for use\u003c\/p\u003e\n\n\u003cp\u003e5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing liquid, and let stand 30 Discard it in seconds and repeat it 5 Times, pat dry.\u003c\/p\u003e\n\n\u003cp\u003e6. Enzyme addition: enzyme labeled reagent is added to each well 50μl, except for blank holes.\u003c\/p\u003e\n\n\u003cp\u003e7. Incubation: Same procedure 3.\u003c\/p\u003e\n\n\u003cp\u003e8. Washing: Same operation 5.\u003c\/p\u003e\n\n\u003cp\u003e9. Color development: Add color developer to each well first A50μl And then adding a color developer B50μl, gently shake and mix evenly, 37℃ Color development protected from light 10 minute.\u003c\/p\u003e\n\n\u003cp\u003e10. Stop: Add stop solution per well 50μl, terminate the reaction (blue immediately turns yellow at this time).\u003c\/p\u003e\n\n\u003cp\u003e11. Measurement: Zero adjustment with blank holes, 450nm The absorbance of each well was measured sequentially by wavelength (OD Value).\u003cbr\u003eThe determination shall be performed after addition of the stop solution 15 Within minutes.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ecalculate\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003eTaking the concentration of the standard as the abscissa, OD The value is the ordinate, draw a standard curve on the coordinate paper, and according to the sample OD The corresponding concentration is found out from the standard curve; Multiply by the dilution factor;\u003cbr\u003eOr use the concentration of the standard substance to OD The linear regression equation of the standard curve is calculated from the value, and the sample OD The value is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses double antigen sandwich method to determine the level of monkey anti-polyethylene glycol IgG antibody (PEG-IgG) in specimens. Coating the microplate with the purified antigen to prepare the solid phase antigen, adding anti-polyethylene glycol IgG antibody (PEG-IgG) to the coated microwells sequentially, and then combining with the HRP-labeled antigen to form an antigen-antibody-enzyme-labeled antigen complex, which is thoroughly washed and colored by adding substrate TMB. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and the anti-polyethylene glycol IgG antibody (PEG-IgG) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of monkey anti-polyethylene glycol IgG antibody (PEG-IgG) in the sample was calculated by the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey anti-polyethylene glycol IgG antibody (PEG-IgG) enzyme-linked immunosorbent kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is used to determine the content of anti-polyethylene glycol IgG antibody (PEG-IgG) in monkey serum, plasma and related fluid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"54\"\u003e\u003cp style=\"text-align: center;\"\u003e Serial number \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"219\"\u003e\u003cp\u003e Component Name \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e Specifications \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"70\"\u003e\u003cp\u003e Serial number \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"213\"\u003e\u003cp\u003e Component Name \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"128\"\u003e\u003cp\u003e Specifications \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e30  Double concentrated Washing Buffer \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e20ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e6\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Color developer  B  Liquid \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e6ml×1\/ Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Enzyme labeled reagent \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e7\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Stop Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Enzyme-labeled coated plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e12  Hole ×8  Strip \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Standard ( 4000pg\/ml ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e0.5ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e9\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e1.5ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"54\"\u003e\u003cp\u003e5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"219\"\u003e\u003cp\u003e Color developer  A  Liquid \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"70\"\u003e\u003cp\u003e10\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"213\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp style=\"text-align: center;\"\u003e2  Sheets \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag.\u003c\/p\u003e\n\u003cp\u003e2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing.\u003c\/p\u003e\n\u003cp\u003e3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples.\u003c\/p\u003e\n\u003cp\u003e4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating.\u003c\/p\u003e\n\u003cp\u003e5. The sealing film is only for one-time use to avoid cross-contamination.\u003c\/p\u003e\n\u003cp\u003e6. Please keep the substrate away from light.\u003c\/p\u003e\n\u003cp\u003e7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e8. All samples, washing solutions and various wastes should be treated as infectious agents.\u003c\/p\u003e\n\u003cp\u003e9. Components of different batch numbers of this reagent shall not be mixed.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, sealed storage at 2-8 ℃, shelf life 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e40pg\/ml –2200pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678480801867,"sku":"abs554433-96T","price":397.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-d2d-elisa-kit","title":"Monkey D2D ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Collection and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe following are general guidelines for sample collection and storage.\u003cbr\u003eSodium azide must not be used as a preservative during all sample collection and storage.\u003c\/p\u003e\n\u003cp\u003e1. Cell Culture Supernatant:\u003cbr\u003eCentrifuge at 4000 rpm for 20 minutes to remove cell particles and aggregates.\u003cbr\u003eStore the supernatant below -20°C and avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Serum:\u003cbr\u003eUse pyrogen- and endotoxin-free tubes, avoiding any cell stimulation during the procedure.\u003cbr\u003eCentrifuge at 4000 rpm for 20 minutes, carefully separate the serum, and store below -20°C.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Plasma:\u003cbr\u003eUse heparin, EDTA, or sodium citrate as an anticoagulant.\u003cbr\u003eCentrifuge at 4000 rpm for 20 minutes, remove the supernatant, and store the plasma below -20°C to avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003eAfter sample collection, if testing cannot be completed in one go, please aliquot and freeze the sample in a single-use aliquot to avoid repeated freezing and thawing.\u003cbr\u003eThaw at room temperature before use to ensure that the sample is evenly and fully thawed.\u003c\/p\u003e\n\u003cp\u003e4. Tissue homogenization:\u003cbr\u003eRinse the tissue with pre-chilled PBS (0.01 M, pH=7.4) to remove residual blood.\u003cbr\u003eWeigh and mince the tissue.\u003cbr\u003eAdd the minced tissue to the corresponding volume of PBS (generally a 1:9 weight-to-volume ratio, for example, 1 g of tissue sample corresponds to 9 mL of PBS.\u003cbr\u003eThe specific volume can be adjusted appropriately according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and collect the supernatant for testing.\u003c\/p\u003e\n\u003cp\u003e5. Cell Extract:\u003cbr\u003eGently wash adherent cells with cold PBS, then digest with trypsin and collect the cells by centrifugation at 1000 × g for 5 minutes.\u003cbr\u003eSuspension cells can be collected directly by centrifugation.\u003cbr\u003eWash the collected cells three times with cold PBS.\u003cbr\u003eResuspend in 150-200 μL of PBS per 1 × 106 cells and disrupt the cells by repeated freeze-thaw cycles (if the cell count is very low, reduce the volume of PBS).\u003cbr\u003eCentrifuge the extract at 1500 × g for 10 minutes, and collect the supernatant for testing.\u003c\/p\u003e\n\u003cp\u003e6. Other biological fluids:\u003cbr\u003eCentrifuge at 1000 × g for 20 minutes to remove impurities and cell debris.\u003cbr\u003eCollect the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e1. Before use, warm all components for at least 60 minutes to ensure they are fully warmed to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Concentrated Wash Solution:\u003cbr\u003eCrystals may form when the concentrated wash solution is removed from the refrigerator.\u003cbr\u003eThis is normal.\u003cbr\u003eHeat in a water bath to completely dissolve the crystals.\u003cbr\u003eDilute the concentrated wash solution with distilled water at a ratio of 1:20: add 1 part concentrated wash solution to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003e3. Substrate:\u003cbr\u003eBefore use, thoroughly mix substrate solutions A and B at a 1:1 volume ratio.\u003cbr\u003eUse within 15 minutes of mixing.\u003c\/p\u003e\n\u003cp\u003e4. Procedure:\u003cbr\u003eBring all reagents and components to room temperature.\u003cbr\u003eDuplicate wells are recommended for standards, controls, and samples.\u003c\/p\u003e\n\u003cp\u003e1. Prepare the working solutions of all components of the kit according to the instructions in the previous instructions.\u003c\/p\u003e\n\u003cp\u003e2. Remove the required strips from the aluminum foil pouch.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to the refrigerator.\u003cbr\u003eSet up standard wells, blank wells, and sample wells.\u003cbr\u003eAdd 50 μL of the standard of varying concentrations to each standard well, leave the blank wells untouched, and add 50 μL of the sample to be tested to the sample wells.\u003c\/p\u003e\n\u003cp\u003e3. Add 100 μL of horseradish peroxidase (HRP)-labeled detection antigen to the standard and sample wells, except for the blank wells.\u003c\/p\u003e\n\u003cp\u003e4. Cover the reaction plate with a sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e5. Remove the sealing film, discard the liquid, and pat dry on absorbent paper.\u003cbr\u003eFill each well with wash solution, let it sit for 20 seconds, then shake off the wash solution and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times.\u003cbr\u003eIf using an automated plate washer, wash the plate according to the machine's operating procedures.\u003cbr\u003eAdding a 30-second soaking period can improve assay accuracy.\u003cbr\u003eAfter washing, pat the plate dry on a clean, lint-free tissue before adding substrate.\u003c\/p\u003e\n\u003cp\u003e6. Thoroughly mix substrates A and B at a 1:1 volume ratio.\u003cbr\u003eAdd 100 μL of the substrate mixture to all wells.\u003cbr\u003eCover the plate with sealing film and incubate at 37°C in a water bath or incubator for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e7. Add 50 μL of stop solution to all wells and read the absorbance (OD) of each well on a microplate reader.\u003c\/p\u003e\n\u003cp\u003eCalculation of Results\u003c\/p\u003e\n\u003cp\u003e1. Using the standard concentration as the horizontal axis and the corresponding absorbance (OD value) as the vertical axis, use computer software and four-parameter logistic curve fitting (4-PL) to create a standard curve equation.\u003cbr\u003eCalculate the sample concentration using the sample absorbance (OD value) using this equation.\u003c\/p\u003e\n\u003cp\u003e2. If the sample is diluted, the concentration measured using the above method must be multiplied by the dilution factor to obtain the final sample concentration.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250414\/d7ed7c6a20f8455e895460a370c31ae2.png\" alt=\"\" width=\"372\" height=\"344\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was less than 10 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA). A monkey D-dimer (D2D) calibrator and the test sample are added to a microplate pre-coated with an anti-monkey D-dimer (D2D) antibody (solid-phase antibody). Then, HRP-labeled monkey D-dimer (D2D) antigen (enzyme-labeled antigen) is added. After incubation and extensive washing to remove unbound components, an immune complex of the solid-phase antibody and enzyme-labeled antigen forms on the solid-phase surface of the microplate. Substrates A and B are added. HRP-catalyzed reaction produces a blue product, which eventually turns yellow with the addition of a stop solution (2M sulfuric acid). The absorbance (OD) is measured at 450 nm on a microplate reader. The absorbance (OD) is negatively correlated with the monkey D-dimer (D2D) concentration in the test sample. The monkey D-dimer (D2D) concentration in the sample can be calculated by fitting the calibrator curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey D-dimer ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eQuantitative determination of monkey D-dimer (D2D) concentration in serum, plasma, and cell culture supernatant.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"198\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"161\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"252\"\u003e\u003cp\u003e\u003cstrong\u003eStorage after opening\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eCalibrators\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e0.3ml\/tube\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃ for 14 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eCoated microplates\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e96T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃14 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eHRP labeled antigen\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eSubstrate solution A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eSubstrate solution B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003e20× concentrated Washing Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"161\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp\u003e2-8℃180 days\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"198\"\u003e\u003cp\u003eSelf-adhesive\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"161\"\u003e\u003cp\u003e2 tablets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"252\"\u003e\u003cp style=\"text-align: center;\"\u003e--\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eThe concentrations of the standards are: 1600, 800, 400, 200, 100, 0 ng\/mL.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Avoid foaming when mixing protein solutions.\u003c\/p\u003e\n\u003cp\u003e2. When adding calibrators and samples, change pipette tips for each calibrator concentration and sample. Common components should be added using a cantilever to avoid cross-contamination.\u003c\/p\u003e\n\u003cp\u003e3. Appropriate incubation times and sufficient washing steps are essential to ensure the accuracy of experimental results.\u003c\/p\u003e\n\u003cp\u003e4. When using an automated plate washer, adding a 30-second soaking step can improve detection accuracy.\u003c\/p\u003e\n\u003cp\u003e5. The substrate solution is a colorless liquid. If it turns blue during storage, it has expired and should not be used.\u003c\/p\u003e\n\u003cp\u003e6. The stop solution should be added in the same order as the substrate solution. After adding the stop solution, the blue substrate product will instantly turn yellow.\u003c\/p\u003e\n\u003cp\u003e7. During the experiment, any remaining strips should be immediately returned to the ziplock bag and sealed (stored dry at low temperature). \u003c\/p\u003e\n\u003cp\u003e8. All liquid components must be shaken thoroughly before use and incubated strictly according to the time, sample volume and sample addition sequence indicated in the instructions. \u003c\/p\u003e\n\u003cp\u003e9. Do not mix with kits or components from other manufacturers. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 ng\/mL - 1600 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678482014283,"sku":"abs554439-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-pcsk-elisa-kit","title":"Monkey PCSK9 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePCSK9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C After overnight 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.)\u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below:\u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution;\u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution;\u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times;\u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times;\u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each dilution step needs to be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (Original solution-1:4) Testing.\u003c\/p\u003e\n\u003cp\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only.\u003cbr\u003eFor actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。\u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 100 ng\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 100 ng\/mL ， 50 ng\/mL ， 25 ng\/mL ， 12.5 ng\/mL ， 6.25 ng\/mL ， 3.13 ng\/mL ， 1.57 ng\/mL Standard dilution ( 0 ng\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250415\/79f84b304b71450fa74899cf8e2f9255.png\" alt=\"\" width=\"600\" height=\"235\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette.\u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam.\u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance.\u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003eng\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e100\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.214\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.127\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e50\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.593\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.506\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.194\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.107\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e12.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.871\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.784\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e6.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.469\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.382\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e3.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.394\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.307\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e1.57\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.221\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.134\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.087\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250415\/238a3ed5684a4a4486f6ce2b186657ec.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  PCSK9 , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 75.6529%; height: 273px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.7774%;\" width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7774%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7805%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.7774%;\" width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7774%;\" width=\"284\"\u003e\u003cp\u003e93-107%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7805%;\" width=\"284\"\u003e\u003cp\u003e100%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.7774%;\" width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7774%;\" width=\"284\"\u003e\u003cp\u003e87-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7805%;\" width=\"284\"\u003e\u003cp\u003e93%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.7774%; text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7774%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e87-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7805%;\" width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e93%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e There will be monkeys added  PCSK9 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e91-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e86-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e88-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e86-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e91-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e95-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.57 ng\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey PCSK9 antibody was coated in a 96-well microplate, and the monkey PCSK9 standard or sample was added to the microwells, so that the monkey PCSK9 protein in the standard or the monkey PCSK9 protein in the sample was bound to the anti-monkey PCSK9 antibody solid on the microplate, then biotinylated anti-monkey PCSK9 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey PCSK9 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey preprotein convertase subtilisin 9 (PCSK9) enzyme-linked immunosorbent assay kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey PCSK9 can be detected in samples and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; height: 802px; width: 57.4558%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.6909%;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 28.8022%;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 45.365%;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.6909%;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 45.365%;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.6909%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.8022%;\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.57-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678482964555,"sku":"abs554441-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-f1-2-elisa-kit","title":"Monkey F1+2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e3.\u003cbr\u003eTissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e4.\u003cbr\u003eCell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eReagent Preparation\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eProcedure\u003cbr\u003e1.\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e2.\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e3.\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e4.\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e5.\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e6.\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e7.\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eCalculation of Experimental Results: Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250414\/3cb7cfa502904e64b51f6de5eb10aa81.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration is less than 0.1 nmol\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to wells pre-coated with a capture antibody targeting monkey prothrombin fragment F1+2 (F1+2). The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey prothrombin fragment F1+2 (F1+2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey prothrombin 1\/2 ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of the content of monkey prothrombin fragment F1+2 (F1+2) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 59.8989%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e12 holes×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e0.3mL*6 tube\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eDetection Antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003e20×Wash Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eDilute according to instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 36.5682%;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.5499%;\" width=\"139\"\u003e\u003cp\u003e2 Sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9747%;\" width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard substances are: 8, 4, 2, 1, 0.5, and 0.25 nmol\/L.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 0.25 nmol\/L – 8 nmol\/L.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678483062859,"sku":"abs554440-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il-17-elisa-kit","title":"Monkey IL-17 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes. Remove the supernatant and store it at -20°C or -80°C. However, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant. Within 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C. Remove the supernatant and test it. Alternatively, store it at -20°C or -80°C. However, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes. Remove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e After removing the reagent kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of 20× Wash Buffer:\u003c\/strong\u003e Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells. Add 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well. Seal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL). Let stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper. Repeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well. Within 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software. Obtain a linear regression equation. Substitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250416\/7dc13f7f11fc48fe92f0c6e6230b5f8e.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003e\n\u003cp style=\"text-align: center;\"\u003eStandard curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable concentration was less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a monkey interleukin-17 (IL-17) capture antibody. The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey interleukin-17 (IL-17) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey interleukin 17 (IL-17) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 1000, 500, 250, 125, 62.5, and 31.25 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.25 pg\/mL – 1000 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678484275275,"sku":"abs554442-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il-1b-elisa-kit","title":"Monkey IL-1β ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes. Remove the supernatant and store it at -20°C or -80°C. However, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant. Within 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C. Remove the supernatant and test it. Alternatively, store it at -20°C or -80°C. However, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes. Remove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e After removing the reagent kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of 20× Wash Buffer:\u003c\/strong\u003e Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells. Add 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well. Seal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL). Let stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper. Repeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well. Within 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software. Obtain a linear regression equation. Substitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250416\/9bbd2b529c3e41009eeb8c467f81f906.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003e\n\u003cp\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration was less than 0.1 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a monkey interleukin-1β (IL-1β) capture antibody. The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of monkey interleukin-1β (IL-1β) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey interleukin 1β (IL-1β) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 80, 40, 20, 10, 5, and 2.5 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2.5 pg\/mL – 80 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678485323851,"sku":"abs554444-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-c-p-elisa-kit","title":"Monkey CP ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the reagent kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250416\/6df2c0747e774f299bdb0837ddc23168.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration was less than 0.1 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with monkey C-peptide (CP) capture antibodies. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The color intensity is positively correlated with the monkey C-peptide (CP) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey C-peptide ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey C-peptide (CP) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 8, 4, 2, 1, 0.5, and 0.25 ng\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.25 ng\/mL – 8 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678486405195,"sku":"abs554445-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il-4-elisa-kit","title":"Monkey IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.)\u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below:\u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution;\u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution;\u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times;\u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times;\u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each dilution step needs to be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (Original solution) Testing.\u003c\/p\u003e\n\u003cp\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only.\u003cbr\u003eFor actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。\u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 500 pg\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 500 pg\/mL ， 250 pg\/mL ， 125 pg\/mL ， 62.5 pg\/mL ， 31.25 pg\/mL ， 15.63 pg\/mL ， 7.82 pg\/mL Standard dilution ( 0 pg\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/c379863402c040e8a66b0526a2c67332.png\" alt=\"\" width=\"600\" height=\"238\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette.\u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam.\u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance.\u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003epg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.996\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.907\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.472\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.383\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e125\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.226\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.137\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e62.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.826\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.737\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e31.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.589\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.5\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e15.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.327\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.238\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e7.82\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.183\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.094\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.089\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/9641c479b3314cca96fb867cb1aabcd1.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  IL4 , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 75.4002%; height: 273px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.7722%;\" width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7722%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7753%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.7722%;\" width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7722%;\" width=\"284\"\u003e\u003cp\u003e94-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7753%;\" width=\"284\"\u003e\u003cp\u003e98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 31.7722%;\" width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7722%;\" width=\"284\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7753%;\" width=\"284\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.7722%; text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7722%; text-align: center;\" width=\"284\"\u003e\u003cp\u003e94-107%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 31.7753%;\" width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e100%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e There will be monkeys added  IL4 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e92-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e86-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e91-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.1 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IL4 antibody was coated in a 96-well microplate, and the monkey IL4 standard or sample was added to the microwells respectively, so that the monkey IL4 protein in the standard or the monkey IL4 protein in the sample was bound to the anti-monkey IL4 antibody solid on the microplate, then biotinylated anti-monkey IL4 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL4 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-4;BSF1; BCGF1 ; B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra；Interleukin 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey IL4 can be detected in samples and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; height: 817px; width: 62.5105%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"text-align: center; width: 40.9376%; height: 54px;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 24.1053%; height: 54px;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7969%; height: 54px;\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.82-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678490730571,"sku":"abs554446-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il-8-elisa-kit","title":"Monkey IL-8 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect. The blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes. deposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect. Anticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples. deposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded. It is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice; In order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times). Finally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect. (Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.) \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed. Take the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save. Avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation. Hemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing. Keep the sample at room temperature prior to the experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e If your test sample needs to be diluted, refer to the general dilution principles below: \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Dilution 50 Times: One-step dilution. Take 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution; \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Dilution 100 Times: One-step dilution. Take  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution; \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Dilution 1000 Times: Two-step dilution. Take  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times; \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Dilution 100000 Times: Three-step dilution. Take 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times; \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times. Too small sampling volume can easily cause greater errors in the mixing process, and each dilution step needs to be mixed evenly to avoid foaming. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (Original solution) Testing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only. For actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 1000 pg\/mL ）。 Thereafter, double dilution is carried out to 1000 pg\/mL ， 500 pg\/mL ， 250 pg\/mL ， 125 pg\/mL ， 62.5 pg\/mL ， 31.25 pg\/mL ， 15.63 pg\/mL Standard dilution ( 0 pg\/mL ) is a blank hole. Configure the standard according to the amount you need for later use. The configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/419224839cea461cb43885aec6bcfad7.png\" alt=\"\" width=\"600\" height=\"240\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day. Dilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。 Before use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day. Dilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.   Before use, mix all reagents thoroughly, taking care not to create any foam. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. The user should calculate the number of samples that may be used throughout the trial.   Please reserve enough samples in advance. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Please estimate the concentration before measurement.   If these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance. When reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well. If the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method). Be careful not to have bubbles. When adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate. Cover or coating, 37°C incubation 80 Minutes. To ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times. For each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Discard the liquid in the well and wash the plate 3 Times. For each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Discard the liquid in the well and wash the plate 5 Times. For each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time). The sequence of addition of the terminating solution should be the same as that of the developer as possible. In order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value). The instrument should be preheated before use, and the testing program should be set up. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value. If a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis). At the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values. Due to the different experimental operating conditions ( such as operator, pipetting technology, plate washing technology and temperature conditions, etc. ), the standard curve OD Values will vary. The standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment. Spent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample. It is recommended to use professional curve drawing software such as curve expert 。 \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003epg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e1000\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.204\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.119\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.583\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.498\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.121\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.036\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e125\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.728\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.643\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e62.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.567\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.482\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e31.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.326\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.241\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e15.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.235\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.15\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.085\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/b14f73d7fd8e46d8a258ab3d5d61fad5.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Add monkeys at known concentrations to different samples  IL8 , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e91%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e82-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e89-107%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e There will be monkeys added  IL8 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-106%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e86-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e93-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.3 pg\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IL8 antibody was coated in a 96-well microplate, and the monkey IL8 standard or sample was added to the microwells, so that the monkey IL8 protein in the standard or the monkey IL8 protein in the sample was bound to the anti-monkey IL8 antibody solid on the microplate, then biotinylated anti-monkey IL8 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL8 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-8;CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey IL8 can be detected in samples and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"194\"\u003e\u003cp\u003e96T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"285\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"194\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"285\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678491910219,"sku":"abs554447-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il-10-elisa-kit","title":"Monkey IL-10 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.) \u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below: \u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution; \u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution; \u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times; \u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times; \u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each step of dilution needs to be mixed evenly to avoid foaming. \u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (Original solution) Testing. \u003c\/p\u003e\n\u003cp\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only.\u003cbr\u003eFor actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 1000 pg\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 1000 pg\/mL ， 500 pg\/mL ， 250 pg\/mL ， 125 pg\/mL ， 62.5 pg\/mL ， 31.25 pg\/mL ， 15.63 pg\/mL Standard dilution ( 0 pg\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/1c202d0759464a01aef27bbdd18a70a1.png\" alt=\"\" width=\"600\" height=\"235\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette. \u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette. \u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam. \u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance. \u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions ( such as operator, pipetting technology, plate washing technology and temperature conditions, etc. ), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。 \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003epg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e1000\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.207\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.12\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.579\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.492\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.107\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.02\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e125\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.897\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.81\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e62.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.513\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.426\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e31.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.312\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.225\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e15.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.135\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.048\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.087\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250417\/cb849b5efd614273862c17a860ad507a.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  IL10 , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e98-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e102%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e95-107%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e101%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e96-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e100%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e There will be monkeys added  IL10 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e90-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-106%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e82-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-90%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e85-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e92-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e81-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6.6 pg\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IL10 antibody was coated in a 96-well microplate, and the monkey IL10 standard or sample was added to the microwells, so that the monkey IL10 protein in the standard or the monkey IL10 protein in the sample was bound to the anti-monkey IL10 antibody solid on the microplate, then biotinylated anti-monkey IL10 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL10 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-10;CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor；Interleukin 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey IL10 can be detected in the sample and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"200\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"231\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"200\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"231\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678492696651,"sku":"abs554448-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-bgp-elisa-kit","title":"Monkey BGP\/OCN ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBGP\/OCN\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e```html\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.)\u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below:\u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution;\u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution;\u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times;\u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times;\u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each step of dilution needs to be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (Original solution-1:2) Testing.\u003c\/p\u003e\n\u003cp\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only.\u003cbr\u003eFor actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。\u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 50 ng\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 50 ng\/mL ， 25 ng\/mL ， 12.5 ng\/mL ， 6.25 ng\/mL ， 3.13 ng\/mL ， 1.57 ng\/mL ， 0.79 ng\/mL Standard dilution ( 0 ng\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250424\/e7529a81ce01478882bc344bc2a647ad.png\" alt=\"\" width=\"600\" height=\"239\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed well with a pipette.\u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam.\u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance.\u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003eng\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e50\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.266\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.171\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.593\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.498\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e12.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.228\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.133\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e6.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.757\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.662\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e3.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.563\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.468\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e1.57\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.318\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.223\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e0.79\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.236\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.141\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.095\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250424\/c11a2c69516e4cd296348ed154d6399c.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  OC\/BGP , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e78-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e87%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e90-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e96%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e There will be monkeys added  OC\/BGP The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e88-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e81-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e80-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e98-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e84-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e88-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e89-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n```\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.46 ng\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey OC\/BGP antibody was coated in a 96-well microplate, and the monkey OC\/BGP standard or sample was added to the microwells respectively, so that the monkey OC\/BGP protein in the standard or the monkey OC\/BGP protein in the sample was bound to the anti-monkey OC\/BGP antibody solid on the microplate, then biotinylated anti-monkey OC\/BGP antibody was added, the unbound biotinylated antibody was washed, HRP labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey OC\/BGP protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBGLAP; BGP; OC; OCN; bone gamma-carboxyglutamate protein; Osteocalcin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey OC\/BGP can be detected in samples and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; height: 350px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 22.3506%;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 28.7353%;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.5672%;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3506%;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 42.5672%;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 22.3506%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 28.7353%;\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.78-50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678493646923,"sku":"abs554452-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-pinp-elisa-kit","title":"Monkey PINP ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250528\/e52f74795e3245e7887a325a951996c8.png\" alt=\"\" width=\"600\" height=\"317\"\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003eFor reference only: Standard curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration is less than 1.0 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a capture antibody against monkey type I procollagen amino-terminal propeptide (PINP). The sample is incubated and washed thoroughly. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of monkey type I procollagen amino-terminal propeptide (PINP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of the content of monkey type I procollagen amino-terminal propeptide (PINP) in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 200, 100, 50, 25, 12.5, and 6.25 ng\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6.25 ng\/mL – 200 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678494335051,"sku":"abs554455-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il15-elisa-kit","title":"Monkey IL-15 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250430\/dd887577e5344e3d8a58f3c20531ccf5.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003e\u003cp\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable concentration was less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a monkey interleukin-15 (IL-15) capture antibody. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of monkey interleukin-15 (IL-15) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey interleukin 15 (IL-15) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 200, 100, 50, 25, 12.5, and 6.25 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly loaded. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6.25 pg\/mL – 200 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678495350859,"sku":"abs554457-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-pk1b-elisa-kit","title":"Monkey PK1B ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePK1B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Dilution of standard: This kit provides one original standard, which can be diluted in a small test tube according to the following chart.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e200ng\/L\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e5  No. Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl  Addition of the original standard  150μl  Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e100ng\/L\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e4  No. Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl  Of  5  No. Standard addition  150μl  Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e50ng\/L\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e3  No. Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl  Of  4  No. Standard addition  150μl  Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e25ng\/L\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e2  No. Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl  Of  3  No. Standard addition  150μl  Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"154\"\u003e\u003cp\u003e12.5ng\/L\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e\u003cp\u003e1  No. Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"505\"\u003e\u003cp\u003e150μl  Of  2  No. Standard addition  150μl  Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e2. Sample addition: Set up blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested respectively.\u003cbr\u003eAccurate spike of standard on enzyme-labeled coated plate  50μl , first add sample diluent to the sample well to be tested  40μl , then add the sample to be tested  10μl (The final dilution of sample was  5  Times).\u003cbr\u003eAdd the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e3. Incubation: After plate sealing with plate sealing film  37℃ Incubation  30  Minutes.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e4. Solution preparation: will  30  Distilled water for double concentrated washing liquid  30  Time dilution for use\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing liquid, and let stand  30  Discard it in seconds and repeat it  5  Times, pat dry.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e6. Enzyme addition: enzyme labeled reagent is added to each well  50μl , except for blank holes.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e7. Incubation: Same procedure  3 。\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e8. Washing: Same operation  5 。\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e9. Color development: Add color developer to each well first  A50μl And then adding a color developer  B50μl , gently shake and mix evenly, 37℃ Color development protected from light 10  minute .\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e10. Stop: Add stop solution per well  50μl , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e11. Measurement: zeroing with blank holes, 450nm  The absorbance of each well was measured sequentially by wavelength ( OD  Value).\u003cbr\u003eThe determination shall be performed after addition of the stop solution  15  Within minutes.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e calculate \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Taking the concentration of the standard as the abscissa, OD  The value is the ordinate, draw a standard curve on the coordinate paper, and according to the sample\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eOD  The corresponding concentration is found out from the standard curve; Multiply by the dilution factor;\u003cbr\u003eOr use the concentration of the standard substance to  OD  The linear regression equation of the standard curve is calculated from the value, and the sample  OD  Substitute the value into the equation, calculate the sample concentration, and multiply it by the dilution factor,\u003cbr\u003eThat is, the actual concentration of the sample.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses double antibody sandwich method to determine the level of monkey precallikrein 1B (PK1B) in specimens. The microplate was coated with purified monkey precallikrein 1B (PK1B) antibody to make a solid phase antibody. The precallikrein 1B (PK1B) was added to the microwells coated with monoclonal antibody in sequence, and then combined with HRP-labeled precallikrein 1B (PK1B) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB was added to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and precallikrein 1B (PK1B) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of monkey precallikrein 1B (PK1B) in the sample was calculated by the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey PK-1B ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor determination of precallikrein 1B (PK1B) content in monkey serum, plasma and related fluid samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"54\"\u003e\u003cp style=\"text-align: center;\"\u003e Serial number \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"219\"\u003e\u003cp\u003e Component Name \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e Specifications \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"70\"\u003e\u003cp\u003e Serial number \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"213\"\u003e\u003cp\u003e Component Name \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"128\"\u003e\u003cp\u003e Specifications \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e30  Double concentrated Washing Buffer \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e20ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e6\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Color developer  B  Liquid \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e6ml×1\/ Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Enzyme labeled reagent \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e7\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Stop Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Enzyme-labeled coated plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e12  Hole ×8  Strip \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Standard ( 400ng\/L ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e0.5ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"54\"\u003e\u003cp\u003e4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"219\"\u003e\u003cp\u003e Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"70\"\u003e\u003cp\u003e9\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"213\"\u003e\u003cp\u003e Standard dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp\u003e1.5ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"54\"\u003e\u003cp\u003e5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"219\"\u003e\u003cp\u003e Color developer  A  Liquid \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e6ml×1  Bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"70\"\u003e\u003cp\u003e11\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"213\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"128\"\u003e\u003cp style=\"text-align: center;\"\u003e2  Sheets \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003e Specimen requirements \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in -20℃ Store, but avoid repeated freezing and thawing \u003c\/p\u003e\n\u003cp\u003e2. Unable to detect containing  NaN3  Samples, due to  NaN3  Inhibition of horseradish peroxidase ( HRP ) activity. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag.\u003c\/p\u003e\n\u003cp\u003e2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing.\u003c\/p\u003e\n\u003cp\u003e3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples.\u003c\/p\u003e\n\u003cp\u003e4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating.\u003c\/p\u003e\n\u003cp\u003e5. The sealing film is only for one-time use to avoid cross-contamination.\u003c\/p\u003e\n\u003cp\u003e6. Please keep the substrate away from light.\u003c\/p\u003e\n\u003cp\u003e7. Operate in strict accordance with the instructions, and the test results must be determined based on the microplate reader reading.\u003c\/p\u003e\n\u003cp\u003e8. All samples, washing solutions and various wastes should be treated as infectious agents.\u003c\/p\u003e\n\u003cp\u003e9. Components of different batch numbers of this reagent shall not be mixed.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4ng\/L-220ng\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678496038987,"sku":"abs554462-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-il33-elisa-kit","title":"Monkey IL-33 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e\u003cbr\u003ePlace the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e\u003cbr\u003eCollect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eTissue Homogenization:\u003cbr\u003eRinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eCell culture supernatant or other biological specimens:\u003cbr\u003eCentrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003eNote:\u003cbr\u003eHemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cp\u003eReagent Preparation\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer:\u003cbr\u003eDilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cp\u003eProcedure\u003cbr\u003e1.\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cp\u003e2.\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cp\u003e3.\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cp\u003e4.\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cp\u003e5.\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cp\u003e6.\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cp\u003e7.\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cp\u003eCalculation of Experimental Results:\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250430\/f20e6a97e3ec4e2f826f19dc5c6faca8.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration was less than 1.0 pg\/ml.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a monkey interleukin-33 (IL-33) capture antibody. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of monkey interleukin-33 (IL-33) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of monkey interleukin 33 (IL-33) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 400, 200, 100, 50, 25, and 12.5 pg\/ml.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 12.5 pg\/ml – 400 pg\/ml.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678496137291,"sku":"abs554458-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-aav-elisa-kit","title":"Monkey AAV ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Collection, Processing, and Storage Methods\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e1. Serum: Use pyrogen- and endotoxin-free tubes. Avoid any cell stimulation during handling. After blood collection, centrifuge at 3000 rpm for 10 minutes to quickly and carefully separate the serum from the red blood cells.\u003cbr\u003e\u003cbr\u003e2. Plasma: Anticoagulate with EDTA, citrate, or heparin. Centrifuge at 3000 rpm for 30 minutes, and remove the supernatant.\u003cbr\u003e\u003cbr\u003e3. Cell Supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and aggregates.\u003cbr\u003e\u003cbr\u003e4. Tissue Homogenate: Add an appropriate amount of saline to the tissue and mash. Centrifuge at 3000 rpm for 10 minutes, and remove the supernatant.\u003cbr\u003e\u003cbr\u003e5. Storage: If samples are not tested immediately after collection, aliquot them into single-use aliquots and freeze at -20°C. Avoid repeated freeze-thaw cycles. Thaw at room temperature, ensuring that the sample is evenly and thoroughly thawed.\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003eDilution of 20× Wash Buffer: Dilute with distilled water at a ratio of 1:20, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePlate Washing Method\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e1. Manual Plate Washing: Shake out all liquid from the wells, fill each well with wash buffer, let stand for 1 minute, shake out all liquid from the wells, pat dry on absorbent paper, and wash the plate five times.\u003cbr\u003e\u003cp\u003e2. Automatic Plate Washer: Fill each well with 350 μL of wash buffer, soak for 1 minute, and wash the plate five times.\u003c\/p\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e\u003cbr\u003e2. Set up negative and positive control wells and sample wells. Add 50 μL of each negative and positive control to each well.\u003cbr\u003e\u003cbr\u003e3. Add 50 μL of the sample to be tested to the sample well.\u003cbr\u003e\u003cbr\u003e4. Then, add 100 μL of horseradish peroxidase (HRP)-conjugated detection antibody to each of the negative and positive control wells and sample wells. Seal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003cbr\u003e\u003cbr\u003e5. Discard the liquid, pat dry on absorbent paper, fill each well with wash buffer, let stand for 1 minute, discard the wash buffer, pat dry on absorbent paper, and repeat this process five times (a microplate washer can also be used).\u003cbr\u003e\u003cbr\u003e6. Add 50 μL of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e\u003cbr\u003e7. Add 50 μL of stop solution to each well and measure the OD value of each well at 450 nm within 15 minutes.\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eResult Interpretation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e1. Test validity: The average OD value of the positive control wells is greater than 1.00; the average OD value of the negative control wells is less than 0.15.\u003cbr\u003e\u003cbr\u003e2. Cut-off value calculation: Cut-off value = average value of negative control wells + 0.15\u003cbr\u003e\u003cbr\u003e3. Negative judgment: If the OD value of the sample is less than the cut-off value, the sample is negative.\u003cbr\u003e\u003cbr\u003e4. Positive judgment: If the OD value of the sample is greater than the cut-off value, the sample is positive.\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eKit Performance\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e1. Accuracy: If the average OD value of the positive control wells is greater than 1.00 and the average OD value of the negative control wells is less than 0.15, the test result is valid.\u003cbr\u003e\u003cbr\u003e2. Specificity: No cross-reaction with other soluble structural analogs.\u003cbr\u003e\u003cbr\u003e3. Repeatability: The intra-plate and inter-plate coefficients of variation were less than 15%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with AAV antigens. After incubation and thorough washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The absorbance (OD) is measured at 450 nm using a microplate reader and compared with the cut-off value to determine the presence of adeno-associated virus (AAV) in the sample.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96\u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"193\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell ELISA Plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells ×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eNegative Control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003ePositive Control\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection Antibody - HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× Wash Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"193\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore the kit at 2-8°C and equilibrate to room temperature for 20 minutes before use. Crystallization of the concentrated wash buffer after removal from the refrigerator is normal. Heat in a water bath to completely dissolve the crystals before use. Strips not used in the experiment should be immediately returned to the ziplock bag and sealed (low-temperature drying) for storage. Pretreated samples do not require dilution; simply add 50 μL. Strictly follow the incubation times, addition volumes, and order specified in the instructions. Shake all liquid components thoroughly before use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678496890955,"sku":"abs554463-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-tsh-elisa-kit","title":"Monkey TSH ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: the sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.)\u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in       -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below:\u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution;\u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution;\u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times;\u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times;\u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each step of dilution needs to be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C。\u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 12 uIU\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 12 uIU\/mL ， 6 uIU\/mL ， 3 uIU\/mL ， 1.5 uIU\/mL ， 0.75 uIU\/mL ， 0.38 uIU\/mL ， 0.19 uIU\/mL Standard dilution ( 0 uIU\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250530\/1354ac6f2b7c45e0a2f6091f248793de.png\" alt=\"\" width=\"600\" height=\"244\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette.\u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam.\u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance.\u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert。\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration \u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003e\u003cstrong\u003euIU\/mL\u003c\/strong\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e12\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.19\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.107\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e6\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.688\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.605\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.067\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.984\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e1.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.782\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.699\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e0.75\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.548\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.465\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e0.38\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.319\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.236\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e0.19\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.124\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.041\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.083\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250530\/6eed311075c54a86ac0e6673b63276b7.png\" alt=\"\" width=\"600\" height=\"337\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cstrong\u003e Precision \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Add monkeys at known concentrations to different samples  TSH , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"350\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"355\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"350\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"355\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"350\"\u003e\u003cp\u003e84-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"355\"\u003e\u003cp\u003e90%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"350\"\u003e\u003cp\u003e79-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"355\"\u003e\u003cp style=\"text-align: center;\"\u003e86%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e linear \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e There will be monkeys added  TSH The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"254\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e79-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e80-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e81-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e89-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"254\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e86-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e81-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp\u003e91-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"254\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e85-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e82-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"164\"\u003e\u003cp\u003e92-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"164\"\u003e\u003cp style=\"text-align: center;\"\u003e82-90%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.12 uIU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey TSH antibody was coated in a 96-well microplate, and the monkey TSH standard or sample was added to the microwells, so that the monkey TSH protein in the standard or the monkey TSH protein in the sample was bound to the anti-monkey TSH antibody solid on the microplate, then biotinylated anti-monkey TSH antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and then TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey TSH protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey thyrotropin (TSH) enzyme-linked immunosorbent assay kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey TSH in the sample can be detected and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e96T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.19-12 uIU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678497808459,"sku":"abs554471-96T","price":397.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-ada-elisa-kit","title":"Monkey ADA ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e After removing the reagent kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of 20× Wash Buffer:\u003c\/strong\u003e Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250624\/cba23e9110af4a35a49acf5aa4daf994.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detection concentration was less than 0.1 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with monkey adenosine deaminase (ADA) capture antibodies. The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of monkey adenosine deaminase (ADA) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor in vitro quantitative detection of monkey adenosine deaminase (ADA) content in serum, plasma, tissue homogenate and related liquid samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 16, 8, 4, 2, 1, and 0.5 ng\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 4°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 ng\/mL – 16 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678498660427,"sku":"abs554480-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-cor-elisa-kit","title":"Monkey Cor ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e 1. Sample collection preparation and storage \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.) \u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or 24 Hourly urine, 2000×g Centrifugation 15 The supernatant was collected after minutes and the sample was saved At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then 2-8°C, 1000×g Centrifugation 15 Minutes, take the supernatant to detect, or sub-package -20°C Save.\u003cbr\u003eAvoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment. \u003c\/p\u003e\n\u003cp\u003e3. If the concentration of the test substance in your sample is higher than the highest value of the standard substance, please dilute it according to the actual situation (it is recommended to do it first Pre-experiments to determine the dilution factor). \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 2. Sample dilution principle \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below: \u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Time dilution. \u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Time dilution. \u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake 5 μL  Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take 5 μL  20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times. \u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times. \u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each dilution step needs to be mixed evenly to avoid foaming. \u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 3. Preparation work before testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 200 ng\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 200 ng\/mL ， 100 ng\/mL ， 50 ng\/mL ， 25 ng\/mL ， 12.5 ng\/mL ， 6.25 ng\/mL ， 3.13 ng\/mL 。\u003cbr\u003eStandard dilution ( 0 ng\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250717\/df1e52e12adf4e02b337d84063cf112e.png\" alt=\"\" width=\"600\" height=\"238\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotin conjugate working solution (1x) : Centrifuge before opening the bottle.\u003cbr\u003eDilute with biotin conjugate diluent immediately before use, and prepare according to the pre-calculated total amount required for each experiment before dilution ( Per well 50 μL) , the actual preparation should be more prepared 0.1-0.2 mL 。\u003cbr\u003eSuch as 10 μL Biotin conjugate plus 990 μL The proportion of biotin conjugate dilution is formulated, gently mixed, and formulated within one hour before use. \u003c\/p\u003e\n\u003cp\u003e5. Streptomycin - Horseradish peroxidase conjugate working solution (1x) : Centrifuge before opening the bottle.\u003cbr\u003eDilute with enzyme conjugate diluent immediately before use, and prepare according to the pre-calculated total amount required for each experiment before dilution ( Per well 100 μL) , the actual preparation should be more prepared 0.1-0.2 mL 。\u003cbr\u003eSuch as 10 μL Enzyme conjugate plus 990 μL Prepare the proportion of the enzyme conjugate diluent, gently mix well, and prepare within one hour before use. \u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 4. Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam. \u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance. \u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 5. Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e2. Sample addition: Standard holes and sample holes to be tested are set respectively.\u003cbr\u003eAdd standard or sample to be tested 50 μL , be careful not to have bubbles, add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and then add biotin conjugate to each well (1 x) 50 μL Gently shaking and mixing evenly, adding a cover or film to the enzyme label plate, 37°C Incubation 1 Hours. \u003c\/p\u003e\n\u003cp\u003e3. To ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e4. Incubation 1 After hours, discard the liquid in the hole, spin dry, and wash the plate 3 Times, add washing solution to each well (1 x) 200 μL , each soak 1-2 Minutes, tumble dry. \u003c\/p\u003e\n\u003cp\u003e5. Then add streptomycin to each well -HRP(1 x) 100 μL Gently shaking and mixing evenly, adding a cover or film to the enzyme label plate, 37°C Incubation 1 Hours. \u003c\/p\u003e\n\u003cp\u003e6. Discard the liquid in the hole, spin dry, wash the plate 5 Times, add washing solution to each well (1 x) 200 μL , each soak 1-2 Minutes, tumble dry. \u003c\/p\u003e\n\u003cp\u003e7. Add each well sequentially TMB Color developer 90 μL ， 37°C Color development protected from light 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e8. Add stop solution to each well sequentially 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the stop solution should be the same as that of the substrate solution as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e9. Use a microplate reader in 450 nm The optical density of each well was measured sequentially by wavelength (OD Value ) 。\u003cbr\u003eAfter addition of stop solution 5 Test within minutes. \u003c\/p\u003e\n\u003cp\u003e10.* Samples may need to be diluted. Please see the sample processing section. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 6. Result calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Competition Law Standards and Samples OD Values can be directly substituted into the calculation.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。 \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"413\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration \u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003e\u003cstrong\u003eng\/mL\u003c\/strong\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"413\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e200\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e0.237\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e100\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e0.427\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e50\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e0.715\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e0.967\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e12.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e1.258\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e6.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e1.596\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e3.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp\u003e1.877\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"413\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"413\"\u003e\u003cp style=\"text-align: center;\"\u003e2.265\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250717\/ac185e0be524425aa9160aacd98bb373.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cstrong\u003e Precision \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Add monkeys at known concentrations to different samples Cor , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e80-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e87%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e86-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e linear \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e There will be monkeys added Cor The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e89-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e82-90%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e82-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e92-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e83-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.31 ng\/mL\t \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of competition law. A microwell plate was coated with purified monkey Cor to prepare a solid phase carrier, sample or standard substance and biotin-labeled antibody were sequentially added into the microwell coated with monkey Cor, and streptomycin-HRP was added after the reaction. After thorough washing, color was developed with the substrate TMB. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a negative correlation between the depth of color and the monkey Cor in the sample. The sample concentration was calculated by measuring the absorbance (OD value) with a microplate reader at a wavelength of 450 nm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey Cortisol  ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey Cor can be detected in the sample and does not cross-react with other related substances.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"249\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"115\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"216\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e12  Strip  × 8  Hole \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Biotin conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e60 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Biotin conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"249\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"115\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate biotin conjugate. The concentrated enzyme conjugate is small in volume and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotin conjugate working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678499545163,"sku":"abs554484-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-anti-peg-igm-elisa-kit","title":"Monkey anti-PEG  IgM ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e 1. Sample collection and storage \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003cstrong\u003e Serum, \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWhole Blood Samples Hold at Room Temperature 2 Hour or 2-8°C Overnight.\u003cbr\u003e1000×g Centrifugation 20 Minutes, take the supernatant.\u003cbr\u003eCan be tested immediately, or frozen in packages according to one-time usage - 20°C Or -80°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003cstrong\u003e Plasma, \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAnticoagulants recommended EDTA-Na2\/K2, after sample collection 30 Within minutes 2-8°C,\u003cbr\u003e1000×g Centrifugation 15 Minutes, take the supernatant.\u003cbr\u003eCan be tested immediately, or frozen in packages according to one-time usage -20°C Or -80°C.\u003cbr\u003ePlease refer to the sample preparation guidelines for the use and selection of other anticoagulants.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3\u003c\/strong\u003e\u003cstrong\u003e Tissue samples, \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTissue samples are generally made into tissue homogenates, and the processing methods are as follows:\u003c\/p\u003e\n\u003cp\u003e1 Placing the target tissue on ice, using pre-cooled PBS Buffer (0.01M, pH=7.4) Wash to remove residual blood, weigh and use later.\u003c\/p\u003e\n\u003cp\u003e2 Grinding the tissue homogenate with the lysate on ice.\u003cbr\u003eThe volume of lysate added depends on the weight of the tissue.\u003cbr\u003eGenerally, each 1g Use of tissue fragments 9ml Lysate.\u003cbr\u003eIt is also recommended to add protease inhibitors to the lysate, such as 1mM PMSF.\u003c\/p\u003e\n\u003cp\u003e3 Can be reused for ultrasonic crushing or repeated freezing and thawing for further treatment (In the process of ultrasonic crushing, ice bath is needed to cool down;\u003cbr\u003eRepeated freeze-thaw method can be repeated 2 Time).\u003c\/p\u003e\n\u003cp\u003e4 Dissolving the prepared homogenate in 5000×g Centrifugation 5 Minutes, retain the supernatant for detection.\u003cbr\u003eOr frozen in packages according to one-time usage -20°C Or -80°C.\u003c\/p\u003e\n\u003cp\u003e5 According to the experimental needs, the total protein concentration of the tissue homogenate sample can be measured first, To facilitate data analysis, recommended BCA Law.\u003cbr\u003eThe total protein concentration is generally adjusted to 1-3mg\/ml Used for ELISA Detection.\u003cbr\u003eCertain tissue samples, such as liver, kidney, and pancreas, contain higher concentrations of endogenous peroxygenase, When the sample concentration is high, it will react with the chromogenic substrate, resulting in false positives.\u003cbr\u003eCan try to use 1%H2O2 Inactivation 15min Re-test.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Note: Commonly used lysate PBS Buffer, or use moderate strength RIPA Lysate.\u003cbr\u003eUsing RIPA When lysing solution, PH The value needs to be adjusted to PH7.3, avoid using containing NP-40, Triton X-100 And DTT The components will seriously inhibit the work of the kit.\u003cbr\u003eRecommended use 50mM Tris+0.9%NaCL+0.1% SDS,PH 7.3.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4\u003c\/strong\u003e\u003cstrong\u003e Cell culture supernatant, \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCollecting the supernatant, 2-8°C, 2500rpm Centrifugation 5min, the clarified cell culture supernatant was collected.\u003cbr\u003eUse immediately for testing, or dispense in a single-use amount -80°C Freeze for later use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003cstrong\u003e Cell lysate, \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Collection and lysis of suspended cells: 2-8°C, 2500rpm Centrifugation 5min, collecting the cells.\u003cbr\u003eAdd the pre-cooled PBS Gently mix and clean, 2-8°C, 2500rpm Centrifugation 5min, collecting the cells.\u003cbr\u003ejoin 0.5-1ml Cell lysate and appropriate amount of protease inhibitor (Such as PMSF, working concentration 1mmol\/L), placed on ice, lysed 30min-1h, Or cooperate with ultrasonic crushing.\u003c\/p\u003e\n\u003cp\u003e2 Collection and lysis of adherent cells: aspirate the supernatant and add pre-cooled PBS Wash three times.\u003cbr\u003ejoin 5-1ml Cell lysate and appropriate amount of protease inhibitor (Such as PMSF, working concentration 1mmol\/L), gently scrape off the adherent cells with a cell scraper.\u003cbr\u003eThe cell suspension was transferred to a centrifuge tube, placed on ice, and lysed 30min-1h, or cooperate with ultrasonic crushing.\u003c\/p\u003e\n\u003cp\u003e3 During the cell lysis process, the centrifuge tube can be blown with the gun tip or shaken intermittently to fully lyse the protein, Appears slimy yes DNA, can be disrupted using ultrasonic DNA.\u003cbr\u003e(Or with ultrasound 3-5mm Probe, Power 150-300W, Sonicated samples on ice, working 1-2 Seconds, stop 30 Seconds, 3~5 A cycle.)\u003c\/p\u003e\n\u003cp\u003e4 Complete cracking or ultrasonic crushing, 2-8°C, 10000rpm Centrifugation 10min, the supernatant is moved into EP In tubes, immediately used for testing, or dispensed into one-time usage -80°C Freeze for later use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Note: The precautions are the same as for tissue samples.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6\u003c\/strong\u003e\u003cstrong\u003e Other biological samples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8°C, 1000×g Centrifuge samples 20 Minutes.\u003cbr\u003eCollect the supernatant for immediate detection, or press The primary usage amount is divided into -80°C Freeze for later use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Other precautions for samples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 The blood collection tubes shall be disposable endotoxin-free tubes.\u003cbr\u003eAvoid the use of hemolyzed, hyperlipidemic samples.\u003c\/p\u003e\n\u003cp\u003e2 Optimal storage conditions of samples: 2-8°C Storage should be less than 5 Day, -20°C Should not exceed 6 Months, -80°C Should not exceed 2 Years, beyond the above time should be kept in liquid In nitrogen.\u003cbr\u003eIn order to reduce ice crystals when frozen specimens are thawed (0°C) Damage to the sample shall be adopted 15-25°C Quickly melting in a water bath, centrifuging to remove the precipitate after melting, Mix well and use for testing.\u003c\/p\u003e\n\u003cp\u003e3 The detection range of the kit is not equivalent to the concentration range of the test substance in the sample.\u003cbr\u003eIf the concentration of the test substance in the sample is too high or too low, please dilute or concentrate the sample appropriately.\u003c\/p\u003e\n\u003cp\u003e4 If the sample tested is special and there is no reference data, it is recommended to do pre-experiment to verify its validity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 2. Recommended sample dilution plan\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Serum \/ Matrix components in plasma can affect test results and need to be diluted with sample diluent at least 2 Times (1\/2)!\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e If your model group sample requires a different dilution ratio\u003c\/strong\u003e Please refer to the general dilution protocol below (This protocol is a dilution protocol that does not set up double wells for testing.\u003cbr\u003eWhen you need to set up a double hole, please add the sample and diluent volume x Number of complex holes):\u003c\/p\u003e\n\u003cp\u003eDilution 2 Times (1\/2): One-step dilution.\u003cbr\u003eTake 60ul Sample addition 60ul In the sample dilution, mix gently.\u003cbr\u003eDilution 5 Times (1\/5): One-step dilution.\u003cbr\u003eTake 24ul Sample addition 96ul In the sample dilution, mix gently.\u003c\/p\u003e\n\u003cp\u003eDilution 10 Times (1\/10): One-step dilution.\u003cbr\u003eTake 12ul Sample addition 108ul In the sample dilution, mix gently.\u003cbr\u003eDilution 20 Times (1\/20): One-step dilution.\u003cbr\u003eTake 6ul Sample addition 114ul In the sample dilution, mix gently.\u003c\/p\u003e\n\u003cp\u003eDilution 50 Times (1\/50): One-step dilution.\u003cbr\u003eTake 3ul Samples and 47ul Normal saline (I.e 0.9% Sodium chloride) join 100ul In the sample dilution, mix gently.\u003cbr\u003eDilution 100 Times (1\/100): One-step dilution.\u003cbr\u003eTake 3ul Samples and 177ul Normal saline addition 120ul In the sample dilution, mix gently.\u003c\/p\u003e\n\u003cp\u003eDilution 1000 Times (1\/1000): Two-step dilution, you can dilute first 50 Times (This step is entirely diluted with saline), re-dilution 20 Times.\u003cbr\u003eGently mix well.\u003c\/p\u003e\n\u003cp\u003eDilution 10000 Times (1\/10000): Two-step dilution, you can dilute first 100 Times (This step is entirely diluted with saline), re-dilution 100 Times.\u003cbr\u003eGently mix well.\u003c\/p\u003e\n\u003cp\u003eDilution 100000 Times (1\/100000): Three-step dilution, you can dilute first 50 Times, re-dilute 20 Times (The first two steps are all diluted with normal saline), final dilution 100 Times.\u003cbr\u003eGently mix well.\u003c\/p\u003e\n\u003cp\u003eNote: The amount of liquid taken during each dilution step is not less than 3ul, the dilution factor is not more than 100 Times.\u003cbr\u003eEach step of dilution should be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 3. Reagent preparation before testing\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eahead of schedule 20 Minutes remove the kit from the refrigerator and equilibrate to room temperature (18-25°C).\u003cbr\u003eIf the kit needs to be used multiple times, please take out only the enzyme-labeled slats and standards required for this experiment, and store the remaining enzyme-labeled slats and standards according to the specified conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003cstrong\u003e Lotion, :\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWith deionized water or distilled water (The recommended resistivity is 18MΩ Ultrapure water) Will 30ml Concentrated wash (48T For 15ml) Diluted to 750ml(48T For 375ml) And mix well.\u003cbr\u003eOr according to the requirements of the experiment, take an appropriate amount of concentrated washing liquid and dilute to 25 Double volume and mix well, return the unused solution 2-8°C.\u003c\/p\u003e\n\u003cp\u003eIf crystals are formed in the concentrated washing liquid, they may be 40°C Heating in water bath (The heating temperature should not exceed 50°C), until the crystals are completely dissolved, mix well and use.\u003cbr\u003eThe prepared lotion is best used on the same day.\u003cbr\u003eIf you can't use it up, you can keep it in 2-8°C, not more than 48 Hours.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003cstrong\u003e Standard, :\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Standard quality control in 1000×g Centrifugation 1 Minutes, allowing the liquid to flow to the bottom of the tube.\u003cbr\u003eMarked as Zero tube.\u003c\/p\u003e\n\u003cp\u003e2 Gradient dilution: separately 7 1 EP Tubes, respectively labeled as 1\/2, 1\/4, 1\/8, 1\/16, 1\/32, 1\/64 And blank.\u003cbr\u003eFirst in each EP Add separately into the tube 0.3ml Sample dilution of.\u003cbr\u003eTake again 0.3ml Zero tube Standard solution is added to 1\/2 Tube, mix thoroughly.\u003cbr\u003eTake again 0.3ml 1\/2 Tube standard solution to 1\/4 Tube, mix thoroughly.\u003cbr\u003eTake again 0.3ml 1\/4 Tube standard solution to 1\/8 Tube, mix thoroughly, and so on.\u003cbr\u003eattention Blank EP Only the sample dilution is in the tube.\u003cbr\u003eAt this point, from Zero tube Tube to blank Take care of this 8 1 EP The concentration of the standard in the tube is 20ng\/ml, 10ng\/ml, 5ng\/ml, 2.5ng\/ml, 1.25ng\/ml, 0.625ng\/ml, 0.312ng\/ml, 0ng\/ml.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Note: Dissolved tube zero standard, please store in 2-8°C, and in 12 Use within hours.\u003cbr\u003eFor other diluted gradient standard working solution, please refer to 2 Use within hours.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3 HRP- Antigen Working Solution:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre-experiment 30 Ready within minutes, ready-to-use, not suitable for long-term storage.\u003c\/p\u003e\n\u003cp\u003e1 Calculate the total volume of working fluid required: 100ul\/ Hole × Number of holes.\u003cbr\u003e(It is best to prepare more than the total volume 100ul-200ul Amount of)\u003c\/p\u003e\n\u003cp\u003e2 1000×g Low speed centrifugation 1 Minutes, will concentrate HRP- The antigen was collected to the bottom of the tube.\u003c\/p\u003e\n\u003cp\u003e3 Press with antigen diluent 1\/100 Dilution and concentration HRP- Antigen, mix well.\u003cbr\u003e(If will 10ul concentrate HRP- Antigen Addition 990ul In antigen dilution)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 4. Operation steps\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWhen diluting samples and reagents, they should be thoroughly mixed.\u003cbr\u003eIt is recommended that a standard curve be plotted for each test.\u003c\/p\u003e\n\u003cp\u003e1 Set standard wells, sample wells, blank wells and record their positions.\u003cbr\u003eIn order to reduce the experimental error, it is recommended to set up double holes for standards and samples.\u003cbr\u003e\u003cstrong\u003e Wash before adding standards and samples board 2 Times.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2 \u003cstrong\u003e Sample addition:\u003c\/strong\u003e Add to the standard well 100ul Each gradient standard was added to the sample well 100ul Moderately diluted sample to be tested, added to the blank well 100ul Sample dilution.\u003cbr\u003eApply a laminating film and apply it in 37°C Static incubation 90 Minutes.\u003cbr\u003e(Add the solution to the bottom of the plate and gently shake it Mix evenly and avoid contact with the pipe wall and blistering as much as possible.)\u003c\/p\u003e\n\u003cp\u003e3 \u003cstrong\u003e Wash board 3 Time:\u003c\/strong\u003e Remove the coating film, suck off or shake off the liquid in the enzyme labeled plate, and pat it on clean absorbent paper 2-3 Times.\u003cbr\u003eAdd wash buffer per well 350ul, immersion 1 Minutes, discard the liquid in the hole and pat it on absorbent paper 2-3 Times.\u003cbr\u003eRepeat this plate washing step 3 Times.\u003c\/p\u003e\n\u003cp\u003e4 \u003cstrong\u003e Add HRP- Antigen Working Solution:\u003c\/strong\u003e Add to each well 100ul HRP- Antigen Working Fluid.\u003cbr\u003eApply a laminating film, 37°C Static incubation 30 Minutes.\u003c\/p\u003e\n\u003cp\u003e5 \u003cstrong\u003e Wash board 5 Time:\u003c\/strong\u003e Remove the coating and wash the plate with wash buffer 5 Time, method reference steps 3.\u003c\/p\u003e\n\u003cp\u003e6 \u003cstrong\u003e Add TMB Chromogenic substrate:\u003c\/strong\u003e Add to each well 90ul TMB Chromogenic substrate, coated with film, in 37°C Static incubation in the dark 10-20 Minutes.\u003cbr\u003eTurn on the plate reader to preheat 15min.\u003cbr\u003e(Note: Do not use the formulation HRP Loading tank for the conjugate.\u003cbr\u003eColor development According to the actual change of color, the reaction time can be shortened or extended, but it cannot exceed 30 Minutes.\u003cbr\u003eWhen appear in the standard hole \u003cu\u003e Better blue gradient \u003c\/u\u003e The reaction may be terminated.\u003cbr\u003eThe color rendering intensity is not easy to be too weak or too strong.\u003cbr\u003ePlease refer to the relevant documents and QR codes on the second page of the manual for accurate control of color rendering methods)\u003c\/p\u003e\n\u003cp\u003e7 \u003cstrong\u003e Add reaction stop solution:\u003c\/strong\u003e After color development, \u003cu\u003e Hole Inner Liquid not Can Abandon Drop\u003c\/u\u003e, add to each well 50ul Reaction stop solution.\u003cbr\u003eThe color will instantly change from blue to yellow.\u003cbr\u003eSequence and addition of stop solution TMB The order of the substrates is the same.\u003c\/p\u003e\n\u003cp\u003e8 \u003cstrong\u003eOD Measurement of values:\u003c\/strong\u003e Immediately use a microplate reader in 450nm Read at OD450 Numerical value.\u003cbr\u003e(If your microplate reader has a selectable correction wavelength, set to 570nm Or 630nm.\u003cbr\u003eThe calibration reading is OD450 Minus the value of OD570 Or OD630 The value of.\u003cbr\u003eThis method corrects and removes non-chromogenic substances OD Value, Thereby obtaining more accurate detection results.\u003cbr\u003eIf the plate reader does not 570nm Or 630nm Wavelength, you can use the original OD450 Value.)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 5. Result calculation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Take the average of standard and sample replicate wells OD450 Value (using raw OD450 Value or correction reading value), subtract the blank hole OD450 Value, as the calculated value.\u003c\/p\u003e\n\u003cp\u003e2 Taking concentration as the abscissa, OD450 Values are ordinates, and four-parameter equations can be used 4PL Draw a standard curve (Remove the values of blank holes when drawing).\u003cbr\u003eYou can also use the mapping software that comes with the plate reader (Such as Thermo FC Model plate reader SkanIt RE Software) Or Curve Expert 1.3 or 1.4 Professional Software (Our website can be downloaded and used for free) Draw the standard curve.\u003c\/p\u003e\n\u003cp\u003e3 The sample's OD450 By substituting the value into the standard curve, the concentration value of the sample can be calculated.\u003cbr\u003eIf the sample has been diluted, multiply it by the corresponding dilution factor.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Experimental data and standard curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis product has been tested by the quality control department and meets the performance requirements of the instruction manual.\u003cbr\u003e(Laboratory humidity is 20%-60%, the temperature is 18°C -25°C.\u003cbr\u003eBefore color development TMB Balance to 37°C After adding enzyme labeled plate wells, 37°C Incubate in the dark 15 Minutes.)\u003c\/p\u003e\n\u003cp\u003eDue to differences in specific experimental environment and operation, the following experimental data and standard curves are for reference only, and experimenters need to establish standard curves according to their own experiments.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto; width: 100%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp style=\"text-align: center;\"\u003eSTD.(ng\/ml)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003eOD-1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003eOD-2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003eAverage\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003eCorrected\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.078\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.08\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.079\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e0.312\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.134\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.138\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.136\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0.057\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e0.625\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.186\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.192\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.189\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0.11\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e1.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.309\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.317\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.313\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0.234\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e2.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.559\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.575\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.567\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0.488\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e0.941\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e0.969\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e0.955\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e0.876\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e10\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e1.479\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e1.521\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e1.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp\u003e1.421\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 24.0177%;\" width=\"259\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.3498%;\" width=\"176\"\u003e\u003cp\u003e2.041\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.4404%;\" width=\"177\"\u003e\u003cp\u003e2.101\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 16.6598%;\" width=\"177\"\u003e\u003cp\u003e2.071\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4208%;\" width=\"206\"\u003e\u003cp style=\"text-align: center;\"\u003e1.992\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIn-plate precision: low, medium and high concentration samples are in the same 1 On-block plate detection 20 Times.\u003c\/p\u003e\n\u003cp\u003eInter-plate precision: Low, medium and high concentration samples are respectively in 3 On-block plate detection 20 Times.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"166\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Category \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" colspan=\"3\" width=\"404\"\u003e\u003cp\u003e\u003cstrong\u003e Intraplate coefficient of variation \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" colspan=\"3\" width=\"425\"\u003e\u003cp\u003e\u003cstrong\u003e Interplate coefficient of variation \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"166\"\u003e\u003cp\u003e Sample \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"120\"\u003e\u003cp\u003e1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"166\"\u003e\u003cp\u003e Quantity \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"120\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e20\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"166\"\u003e\u003cp\u003e Average (ng\/ml)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"120\"\u003e\u003cp\u003e0.74\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e2.87\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e11.52\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e0.84\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e3.14\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e12.04\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"166\"\u003e\u003cp\u003e Standard deviation \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"120\"\u003e\u003cp\u003e0.04\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e0.15\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e0.59\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"141\"\u003e\u003cp\u003e0.04\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e0.15\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp\u003e0.38\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"166\"\u003e\u003cp\u003e Coefficient of variation (%)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"120\"\u003e\u003cp\u003e5.22\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"141\"\u003e\u003cp\u003e5.11\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e5.11\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"141\"\u003e\u003cp\u003e5.31\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"142\"\u003e\u003cp\u003e4.89\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"142\"\u003e\u003cp style=\"text-align: center;\"\u003e3.16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCombine a certain amount of Anti-PEG IgM Added to the sample, and the measured values were added to the sample by comparing the measured values with Anti-PEG IgM The expected amounts of were compared to calculate the recovery.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"368\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Sample Type \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"334\"\u003e\u003cp\u003e\u003cstrong\u003e Recovery Range (%)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"294\"\u003e\u003cp\u003e\u003cstrong\u003e Average recovery (%)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"368\"\u003e\u003cp\u003e Serum (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"334\"\u003e\u003cp\u003e85-105\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"294\"\u003e\u003cp\u003e95\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"368\"\u003e\u003cp\u003eEDTA Plasma (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"334\"\u003e\u003cp\u003e85-105\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"294\"\u003e\u003cp\u003e95\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"368\"\u003e\u003cp\u003e Heparin plasma (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"334\"\u003e\u003cp\u003e85-103\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"294\"\u003e\u003cp style=\"text-align: center;\"\u003e95\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e linear\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe appropriate concentration will be added Anti-PEG IgM The samples were diluted separately 2 Times, 4 Times, 8 Times to obtain the recovery range.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"357\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"246\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"200\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"194\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"357\"\u003e\u003cp\u003e Serum ( (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"246\"\u003e\u003cp\u003e88-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e88-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e91-100%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"357\"\u003e\u003cp\u003eEDTA Plasma (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"246\"\u003e\u003cp\u003e94-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"200\"\u003e\u003cp\u003e88-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp\u003e85-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"357\"\u003e\u003cp\u003e Heparin plasma (n=10)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"246\"\u003e\u003cp\u003e86-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"200\"\u003e\u003cp\u003e85-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"194\"\u003e\u003cp style=\"text-align: center;\"\u003e86-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe unopened kit is available in 37°C And 2-8°C Stability experiments were performed and stability data were obtained.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 0px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.3687%;\" width=\"362\"\u003e\u003cp style=\"text-align: center;\"\u003e Test kit (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 22.371%;\" width=\"235\"\u003e\u003cp\u003e37°C One month \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.0225%;\" width=\"200\"\u003e\u003cp\u003e2-8°C Six months \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 18.5455%;\" width=\"195\"\u003e\u003cp\u003e2-8°C Twelve months \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 34.3687%;\" width=\"362\"\u003e\u003cp\u003e Average (%)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 22.371%;\" width=\"235\"\u003e\u003cp\u003e80\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 19.0225%;\" width=\"200\"\u003e\u003cp\u003e95-100\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.5455%;\" width=\"195\"\u003e\u003cp style=\"text-align: center;\"\u003e85-98\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.188ng\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses capture ELISA detection method, and the experimental duration is 3 hours. The plates in the kit have been pre-coated with Anti Monkey IgM (u-chain). HRP-antigen was used as the detection source. Add the standard substance and moderately diluted sample to be tested into the corresponding wells respectively, and wash away the unbound components after incubation. HRP-antigen is then added, which binds to Anti-PEG IgM already bound to Anti Monkey IgM (u-chain). After washing off the unbound components, TMB chromogenic substrate was added. TMB became blue under the catalysis of horseradish peroxidase (HRP), and turned yellow after addition of reaction stop solution. The OD value was measured with a microplate reader at a wavelength of 450 nm, and the concentration of Anti-PEG IgM in the sample was calculated by drawing a standard curve. There is a direct proportion between the concentration of the target substance and the OD450 value.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey anti-PEG(Polyethylene glycol) IgM ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is used for quantitative detection of Anti-PEG IgM in serum, plasma, cell culture supernatant or other biological samples in vitro.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Serial number \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"311\"\u003e\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Name \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"127\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003cstrong\u003e(96T)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"362\"\u003e\u003cp\u003e\u003cstrong\u003e Save conditions after opening \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003eElisa  Enzyme plate ( Detachable )\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e8  Hole ×12  Strip \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"362\"\u003e\n\u003cp\u003e Place the unused holes in a zippered aluminum foil bag and add desiccant and store sealed. Available at  2-8°C  Save  1\u003c\/p\u003e\n\u003cp\u003e Months; In -20°C  Save  12  Months. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1ml\/2  Vials \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"362\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e Place the remaining standards in  2-8°C , please do not freeze! \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e concentrate  HRP- Antigen  100X\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1  Vials  120ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"2\" width=\"362\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e2-8°C ( Protect from light )\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003eTMB  Chromogenic substrate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1  Bottle  10ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1  Bottle  20ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"4\" width=\"362\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e2-8°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e6\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e Antigen Diluent \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1  Bottle  10ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e7\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1  Bottle  10ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"67\"\u003e\u003cp\u003e8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"311\"\u003e\u003cp\u003e Concentrated wash  25X\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"127\"\u003e\u003cp\u003e1  Bottle  30ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"67\"\u003e\u003cp\u003e9\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"311\"\u003e\u003cp\u003e Coating \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"127\"\u003e\u003cp\u003e5  Sheets \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"362\"\u003e\u003cp style=\"text-align: center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e Note: The volume of liquid reagent supplied in the reagent bottle is slightly larger than the label indicates. Please use a pipette to measure accurately and dilute accordingly. \u003c\/p\u003e\n\u003cp\u003e Unopened kit, please store in  2-8°C 。 After opening, the storage conditions are shown in the table above: \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure.\u003c\/p\u003e\n\u003cp\u003e2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture).\u003c\/p\u003e\n\u003cp\u003e3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation.\u003c\/p\u003e\n\u003cp\u003e4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds.\u003c\/p\u003e\n\u003cp\u003e5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure.\u003c\/p\u003e\n\u003cp\u003e6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation.\u003c\/p\u003e\n\u003cp\u003e7. Do not reuse disposable tips to avoid cross-contamination.\u003c\/p\u003e\n\u003cp\u003e8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature.\u003c\/p\u003e\n\u003cp\u003e9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 2-8 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.313-20ng\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678500659275,"sku":"abs554489-96T","price":698.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-iga-elisa-kit","title":"Monkey IgA ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIgA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.) \u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e If your test sample needs to be diluted, refer to the general dilution principles below: \u003c\/p\u003e\n\u003cp\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution; \u003c\/p\u003e\n\u003cp\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution; \u003c\/p\u003e\n\u003cp\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times; \u003c\/p\u003e\n\u003cp\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times; \u003c\/p\u003e\n\u003cp\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each dilution step needs to be mixed evenly to avoid foaming. \u003c\/p\u003e\n\u003cp\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 200 µg\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 200 µg\/mL ， 100 µg\/mL ， 50 µg\/mL ， 25 µg\/mL ， 12.5 µg\/mL ， 6.25 µg\/mL ， 3.13 µg\/mL Standard dilution ( 0 µg\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250514\/a18870b2d20443038aa4b6339dbf4f7c.png\" alt=\"\" width=\"600\" height=\"242\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette. \u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette. \u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam. \u003c\/p\u003e\n\u003cp\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance. \u003c\/p\u003e\n\u003cp\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed evenly, and foaming should be avoided as much as possible when mixing evenly.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。 \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003eµg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e200\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.201\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.114\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e100\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.608\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.521\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e50\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.106\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.019\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.933\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.846\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e12.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.614\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.527\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e6.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.357\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.27\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e3.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.226\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.139\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.087\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250514\/c5a64e46b73e4f74a305ce2d280f7091.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cstrong\u003e\u003cbr\u003e Precision \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Add monkeys at known concentrations to different samples  IgA , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e80-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e91-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e linear \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e There will be monkeys added  IgA The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e84-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e83-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e79-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e86-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e83-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e93-107%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e88-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e90-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.03 µg\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IgA antibody was coated in a 96-well microplate, and monkey IgA standards or samples were added to the microwells, so that the monkey IgA protein in the standard or the monkey IgA protein in the sample was bound to the anti-monkey IgA antibody solid on the microplate, then biotinylated anti-monkey IgA antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IgA protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey immunoglobulin A (IgA) enzyme-linked immunosorbent assay kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey IgA can be detected in the sample and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; width: 60%; height: 400px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.27%; text-align: center;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 44.27%;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.27%; text-align: center;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.6161%; text-align: center;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.7217%;\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.13-200 µg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678505803851,"sku":"abs554464-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-igfbp3-elisa-kit","title":"Monkey IGFBP3 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003eSerum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003ePlasma: Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eCentrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection.\u003cbr\u003eThe supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003eTissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e\u003cbr\u003eCell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect them by centrifugation at 1000×g for 5 minutes.\u003cbr\u003eSuspension cells can be collected directly by centrifugation.\u003cbr\u003eWash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately).\u003cbr\u003eDisrupt the cells by repeated freeze-thaw cycles or sonication.\u003cbr\u003eCentrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis.\u003cbr\u003e\u003cbr\u003eCell culture supernatant: Centrifuge at 1000×g for 20 minutes, remove the supernatant, or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003cbr\u003eOther biological fluids: Centrifuge at 1000×g for 20 minutes, and remove the supernatant for analysis.\u003cbr\u003e\u003cbr\u003ePre-Assay Preparation:\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard.\u003cbr\u003eLet stand for 15 minutes to completely dissolve, then gently mix (concentration is 50 ng\/mL).\u003cbr\u003eThen dilute to the following concentrations: 50 ng\/mL, 25 ng\/mL, 12.5 ng\/mL, 6.25 ng\/mL, 3.125 ng\/mL, 1.5625 ng\/mL, 0.78125 ng\/mL, and 0 ng\/mL.\u003cbr\u003eSerial Dilution Method: Take 7 EP tubes and add 500 μL of universal diluent to each tube.\u003cbr\u003ePipette 500 μL of the 50 ng\/mL standard working solution into the first EP tube and mix thoroughly to make a 25 ng\/mL standard working solution.\u003cbr\u003eRepeat this procedure for subsequent tubes.\u003cbr\u003eThe last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube.\u003cbr\u003eSee the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute.\u003cbr\u003eDilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent).\u003cbr\u003ePrepare immediately before use.\u003cbr\u003e4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute.\u003cbr\u003eDilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent).\u003cbr\u003ePrepare immediately.\u003cbr\u003e5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal.\u003cbr\u003eAllow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return to 4°C.\u003cbr\u003e2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells.\u003cbr\u003eAdd 100 μL of universal diluent to the blank wells.\u003cbr\u003eCover with a film and incubate at 37°C for 60 minutes.\u003cbr\u003e(Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate.\u003cbr\u003eThis will reduce the impact of matrix effects on the test results.\u003cbr\u003eThe sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration.\u003cbr\u003eIt is recommended to run replicates for all test samples and standards.)\u003cbr\u003e3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing.\u003cbr\u003eAdd 100 μL of Biotinylated Antibody Working Solution directly to each well.\u003cbr\u003eCover with a film and incubate at 37°C for 60 minutes.\u003cbr\u003e4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well.\u003cbr\u003eLet stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper.\u003cbr\u003eRepeat this process three times (a plate washer can also be used).\u003cbr\u003e5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well.\u003cbr\u003eCover with a film and incubate at 37°C for 30 minutes.\u003cbr\u003e6. Washing: Discard the liquid and wash the plate five times as in step 4.\u003cbr\u003e7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well.\u003cbr\u003eImmediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003eCalculating experimental results:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor.\u003cbr\u003ePlot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest.\u003cbr\u003eMultiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/5df7e4c65d6241a9984548cfb6f17a5f.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with an Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey Insulin Like Growth Factor Binding Protein 3 ELISA Kit ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Concentrated biotinylated antibody ( 100× ）  \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.78-50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678510817355,"sku":"abs554142-96T","price":368.0,"currency_code":"USD","in_stock":true}]},{"product_id":"monkey-saa-elisa-kit","title":"Monkey SAA ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSAA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003cbr\u003e(Tissue homogenates require simultaneous detection of protein concentrations to obtain a more accurate test substance concentration per milligram of protein.)\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Notes \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Principles of sample dilution \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e If your test sample needs to be diluted, refer to the general dilution principles below:\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Dilution 50 Times: One-step dilution.\u003cbr\u003eTake 5 μL Sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Dilution 100 Times: One-step dilution.\u003cbr\u003eTake  5 μL Sample to 495 μL Standard \u0026amp; In the sample dilution, is 100 Double dilution;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Dilution 1000 Times: Two-step dilution.\u003cbr\u003eTake  5 μL Sample to 95 μL Standard \u0026amp; In the sample dilution, is 20 Dilute, and then take  5 μL 20 Double dilute sample to 245 μL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 Times;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Dilution 100000 Times: Three-step dilution.\u003cbr\u003eTake 5 μL Sample to 195 μL Standard \u0026amp; In the sample dilution, is 40 Dilute, and then take 5 μL 40 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Time dilution, and finally take 5 μL 2000 Double dilute sample to 245 μL Standard \u0026amp; In the sample diluent, do 50 Double dilution, total dilution 100000 Times;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. The amount of liquid taken during each dilution step is not less than 3 μL , the dilution factor is not more than 100 Times.\u003cbr\u003eToo small sampling volume can easily cause greater errors in the mixing process, and each step of dilution needs to be mixed evenly to avoid foaming.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. When the dilution factor is very high, you can use it first PBS Dilution, last step using standard in kit \u0026amp; Sample dilution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Sample dilution recommendations \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Normal fresh serum \/ Plasma Sample Recommendation (1:2-1:10) Testing.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Due to individual variations, the recommended dilution factor is for informational purposes only.\u003cbr\u003eFor actual testing, please estimate the concentration range of the sample in advance, and determine the dilution factor of the sample to be tested through pre-experiments.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 200 ng\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 200 ng\/mL ， 100 ng\/mL ， 50 ng\/mL ， 25 ng\/mL ， 12.5 ng\/mL ， 6.25 ng\/mL ， 3.13 ng\/mL Standard dilution ( 0 ng\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250603\/6cd6d6d14e0d4bd3bd9b5e8e43d80c31.png\" alt=\"\" width=\"600\" height=\"239\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Preparation before the experiment \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. All materials and prepared reagents were equilibrated to room temperature prior to use.\u003cbr\u003eBefore use, mix all reagents thoroughly, taking care not to create any foam.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. The user should calculate the number of samples that may be used throughout the trial.\u003cbr\u003ePlease reserve enough samples in advance.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Please estimate the concentration before measurement.\u003cbr\u003eIf these values are not within the standard curve range, the user must determine the optimal sample dilution for their particular experiment.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Of each standard and sample OD Value should be subtracted from the blank hole OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert 。\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"354\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003eng\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e200\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.266\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e2.179\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e100\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.586\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.499\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e50\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.186\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e1.099\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.805\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.718\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e12.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.539\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.452\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e6.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.324\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.237\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"354\"\u003e\u003cp\u003e3.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.213\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp\u003e0.126\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"354\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"240\"\u003e\u003cp\u003e0.087\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"240\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250603\/a2fd3ea254b1467cabdd7b263543edf0.png\" alt=\"\" width=\"600\" height=\"336\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Note : This picture is for reference only \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cstrong\u003e Precision \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e\u003cbr\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to evaluate the precision of the analytical plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e\u003cbr\u003e Add monkeys at known concentrations to different samples  SAA , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e80-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e87%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e90-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp\u003e97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"284\"\u003e\u003cp\u003e86-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"284\"\u003e\u003cp style=\"text-align: center;\"\u003e92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstrong\u003e linear \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e\u003cbr\u003e There will be monkeys added  SAA The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"237\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e95-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e97-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e92-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e85-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"237\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e81-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e96-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e92-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp\u003e82-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"237\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e78-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e86-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"153\"\u003e\u003cp\u003e96-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"153\"\u003e\u003cp style=\"text-align: center;\"\u003e89-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.273 ng\/mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey SAA antibody was coated in a 96-well microplate, and the monkey SAA standard or sample was added to the microwells respectively, so that the monkey SAA protein in the standard or the monkey SAA protein in the sample was bound to the anti-monkey SAA antibody solid on the microplate, then biotinylated anti-monkey SAA antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey SAA protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey SAA(Serum Amyloid A) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey SAA can be detected in samples and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"249\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"115\"\u003e\u003cp\u003e96T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"216\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"249\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"115\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"249\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"115\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"216\"\u003e\u003cp style=\"text-align: center;\"\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.\u003c\/p\u003e\n\u003cp\u003e8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678515404875,"sku":"abs554476-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vb12-elisa-kit","title":"VB12 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003e1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis.\u003cbr\u003e5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles.\u003cbr\u003e6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and assay.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePre-Assay Preparation:\u003c\/strong\u003e\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Preparation of Standard Gradient Working Solution: Add 1mL of Universal Diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration is 5000pg\/mL). Then dilute to the following concentrations: 5000pg\/mL, 2500pg\/mL, 1250pg\/mL, 625pg\/mL, 312.5pg\/mL, 156.25pg\/mL, 78.125pg\/mL, and 0pg\/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 5000pg\/mL standard working solution into the first EP tube and mix thoroughly to make a 2500pg\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day.\u003cbr\u003e4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day.\u003cbr\u003e5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProcedure:\u003c\/strong\u003e\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.)\u003cbr\u003e3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes.\u003cbr\u003e5. Wash: Discard the liquid and wash the plate five times as in step 3.\u003cbr\u003e6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCalculation of experimental results:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eResult evaluation:\u003c\/strong\u003e\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/76c4aaab284943cb9b9e5d093c38c9e4.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal vitamin B12 (VB12) antigen. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the universal vitamin B12 (VB12) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGeneral Vitamin B12ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompetition Law\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate  Biotin- Antibody ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e60uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVitamin B12, also known as cobalamin, is a water-soluble vitamin involved in metabolism. It is a cofactor in DNA synthesis and fatty acid and amino acid metabolism. It is crucial for the normal function of the nervous system through its role in myelin synthesis and plays a vital role in the maturation of red blood cells in the bone marrow. In developed countries, the most common cause of vitamin B12 deficiency is malabsorption due to loss of intrinsic factor (IF), which must be bound to dietary B12 for absorption. A second major cause is age-related decreased gastric acid secretion (hydration), as exposure to gastric acid releases protein-bound vitamins. For similar reasons, long-term antacid therapy, use of proton pump inhibitors, H2 receptor blockers, or other antacids increases the risk of developing the disease. Deficiency may be characterized by limb neuropathy or a blood disorder called pernicious anemia, a megaloblastic anemia. Folate levels in the body may influence the pathological course and symptoms of vitamin B12 deficiency.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e78-5000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678541094987,"sku":"abs554143-96T","price":368.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vancomycin-high-sensitivity-elisa-kit","title":"Vancomycin High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003eSerum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003ePlasma: Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eCentrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection.\u003cbr\u003eThe supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003eTissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e\u003cbr\u003eCell lysis solution: Gently wash adherent cells with pre-cooled PBS, then digest with trypsin, and collect the cells after centrifugation at 1000×g for 5 minutes; suspended cells can be directly collected by centrifugation.\u003cbr\u003eWash the collected cells 3 times with pre-cooled PBS, add 150-200uL PBS for every 1×10^6 cells to resuspend (it is recommended to add protease inhibitors to PBS; if the content is very low, the PBS volume can be appropriately reduced) and break the cells by repeated freezing and thawing or ultrasound.\u003cbr\u003eCentrifuge the extract at 2-8℃, 1500×g for 10 minutes, and take the supernatant for detection.\u003cbr\u003e\u003cbr\u003eCell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003eOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test.\u003cbr\u003e\u003cbr\u003ePre-test preparation:\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration 100 ng\/mL).\u003cbr\u003eThen dilute to the following concentrations: 100 ng\/mL, 50 ng\/mL, 25 ng\/mL, 12.5 ng\/mL, 6.25 ng\/mL, 3.125 ng\/mL, 1.5625 ng\/mL, and 0 ng\/mL.\u003cbr\u003eSerial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube.\u003cbr\u003ePipette 500uL of the 100ng\/mL standard working solution into the first EP tube and mix thoroughly to make a 50ng\/mL standard working solution.\u003cbr\u003eRepeat this procedure for subsequent tubes.\u003cbr\u003eThe last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube.\u003cbr\u003eSee the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use.\u003cbr\u003eDilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day.\u003cbr\u003e4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute.\u003cbr\u003eDilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent).\u003cbr\u003eUse the same day.\u003cbr\u003e5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal.\u003cbr\u003eAllow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells.\u003cbr\u003eAdd 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well.\u003cbr\u003eCover with film and incubate at 37°C for 1 hour.\u003cbr\u003e(Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects.\u003cbr\u003eThe sample concentration should be multiplied by the dilution factor when calculating the final concentration.\u003cbr\u003eIt is recommended to run replicates for all samples and standards.)\u003cbr\u003e3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well.\u003cbr\u003eLet stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper.\u003cbr\u003eRepeat this process three times (a plate washer can also be used).\u003cbr\u003e4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well.\u003cbr\u003eCover with film and incubate at 37°C for 30 minutes.\u003cbr\u003e5. Wash: Discard the liquid and wash the plate five times as in step 3.\u003cbr\u003e6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well.\u003cbr\u003eImmediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003eCalculation of experimental results:\u003cbr\u003eResult evaluation:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value.\u003cbr\u003ePlot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest.\u003cbr\u003eMultiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/7adf905ab4b94e8eb7fdceb862d42ca7.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with the universal species Vancomycin High Sensitivity antigen. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the universal species Vancomycin High Sensitivity in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVancomycin ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompetition Law\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate  Biotin- Antibody ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e60uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVancomycin is a glycopeptide antibiotic used to treat serious infections that have failed all other antibiotics. It acts by inhibiting the second phase of cell wall synthesis in susceptible bacteria, altering cell membrane permeability and selectively inhibiting ribonucleic acid synthesis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678541193291,"sku":"abs554144-96T","price":559.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rabbit-myo-myoglobin-elisa-kit","title":"Rabbit MYO ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eI. Sample Collection, Preparation, and Storage\u003cbr\u003e\u003cbr\u003e1. Serum: After placing whole blood samples at room temperature for 2 hours or at 4°C overnight, centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing. Blood collection tubes should be disposable, pyrogen-free, and endotoxin-free. Store at -20°C or -80°C and avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e2. Plasma: Within 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C. Remove the supernatant for testing. EDTA-Na2 is recommended as the anticoagulant to avoid hemolysis or hyperlipidemia. Store at -20°C or -80°C and avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e3. Tissue Homogenization: Take an appropriate amount of tissue and wash it in pre-chilled PBS (0.01M, pH 7.0-7.2) to remove blood (lysed red blood cells in the homogenate will affect the measurement results). After weighing, mince the tissue and mix it with the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio; the specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS). Pour the mixture into a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or freeze-thawed repeatedly (keep the sonication in an ice bath and repeat the freeze-thaw cycle twice). Finally, centrifuge the homogenate at 5000×g for 5-10 minutes. Remove the supernatant for analysis.\u003cbr\u003e\u003cbr\u003e4. Cell Culture Supernatant: Centrifuge the cell supernatant at 1000×g for 20 minutes to remove impurities and cell debris. Remove the supernatant for testing and store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e5. Urine: Collect the first morning urine (midstream) or 24-hour urine collection. Centrifuge at 2000×g for 15 minutes, collect the supernatant, and store the sample at -20°C. Avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e6. Saliva: Collect the sample using a saliva sample collection tube, then centrifuge at 1000×g for 15 minutes at 2-8°C. Remove the supernatant for testing, or aliquot and store at -20°C. Avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e7. Other biological samples: Centrifuge at 1000×g for 20 minutes, collect the supernatant, and store at -20°C. Avoid repeated freezing and thawing.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. Samples should be clear and transparent, and suspended matter should be removed by centrifugation. Hemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Samples can be stored at 4°C if tested within one week of collection. If testing cannot be performed promptly, aliquot the sample into single-use portions and freeze at -20°C (for testing within one month) or -80°C (for testing within three to six months). Avoid repeated freeze-thaw cycles. Bring the sample to room temperature before experimenting.\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. If the concentration of the test substance in your sample is higher than the highest value of the standard, please dilute it appropriately based on the actual situation (it is recommended to conduct a pilot experiment to determine the dilution factor).\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e2. Pre-Assay Preparation\u003cbr\u003e\u003cbr\u003e1. Remove the test kit from the refrigerator 30 minutes in advance and equilibrate to room temperature.\u003cbr\u003e\u003cbr\u003e2. Dilute 25 μg of concentrated wash buffer to 1 μg of working solution with double-distilled water. Return any unused portion to 4°C.\u003cbr\u003e\u003cbr\u003e3. Standards: Add 1.0 mL of Universal Diluent for Standards \u0026amp; Samples to the lyophilized standard. Tighten the cap and let stand for 10 minutes to fully dissolve. Gently mix to achieve a concentration of 5000 pg\/mL. Perform serial dilutions to 5000 pg\/mL, 2500 pg\/mL, 1250 pg\/mL, 625 pg\/mL, 312.5 pg\/mL, 156.25 pg\/mL, and 78.13 pg\/mL. Use the standard diluent (0 pg\/mL) as a blank well. Prepare the required amount of standard solution and set aside. It is recommended to add the prepared standard solution to the sample within 15 minutes; do not allow it to sit for extended periods.\u003cbr\u003e\u003cbr\u003e4. Biotinylated Antibody Working Solution: Calculate the required volume of biotinylated antibody working solution before use (based on 100 μL\/well; add 100-200 μL more). 15 minutes before use, dilute the concentrated biotinylated antibody (1:100) with Biotinylated Antibody Diluent to the working concentration. Use the same day. The dilution principle is to add 1 μL of concentrated biotinylated antibody to 99 μL of biotinylated antibody diluent and mix thoroughly with a pipette.\u003cbr\u003e\u003cbr\u003e5. Enzyme Conjugate Working Solution: Before the experiment, calculate the required volume for the experiment (based on 100 μL\/well; add 100-200 μL more when preparing). 15 minutes before use, dilute the concentrated HRP enzyme conjugate (1:100) with enzyme conjugate diluent to the working concentration for use that day. The dilution principle is to add 1 μL of concentrated enzyme conjugate to 99 μL of enzyme conjugate diluent and mix thoroughly with a pipette.\u003cbr\u003e\u003cbr\u003e6. TMB Substrate - Use a pipette to aspirate the required volume of solution. Do not pour any remaining solution back into the reagent bottle.\u003cbr\u003e\u003cbr\u003eNotes:\u003cbr\u003e1. Before using the kit, ensure that all components are dissolved and mixed thoroughly. Discard any unused standard after reconstitution.\u003cbr\u003e\u003cbr\u003e2. Concentrated biotinylated antibody and enzyme conjugate solutions are small in size and may disperse throughout the tube during transportation. Centrifuge at 1000 × g for 1 minute before use to allow any liquid on the tube walls or cap to settle to the bottom. Mix the solution by carefully pipetting 4-5 times before use. Prepare the standard, biotinylated antibody working solution, and enzyme conjugate working solution according to the required volume and use the corresponding diluents. Do not mix them.\u003cbr\u003e\u003cbr\u003e3. Concentrated wash solution may crystallize after removal from the refrigerator. This is normal. Dissolve the crystals completely in a water bath or incubator before preparing the wash solution (do not heat above 40°C). The wash solution should be at room temperature when used.\u003cbr\u003e\u003cbr\u003e4. Samples should be added quickly, preferably within 10 minutes each time. To ensure experimental accuracy, it is recommended to use replicates. When pipetting reagents, maintain a consistent order of addition from one well to another. This will ensure the same incubation time for all wells.\u003cbr\u003e\u003cbr\u003e5. During the wash process, any wash solution remaining in the reaction wells should be patted dry on absorbent paper. Do not place filter paper directly into the reaction wells to absorb water. Before reading, be sure to remove any residual liquid and fingerprints at the bottom to avoid affecting the microplate reader reading.\u003cbr\u003e\u003cbr\u003e6. The color developer TMB should be protected from direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If a gradient is already obvious, stop the reaction early to avoid excessive color that affects the microplate reader reading.\u003cbr\u003e\u003cbr\u003e7. The test tubes and reagents used in the experiment are disposable. Reuse is strictly prohibited, otherwise it will affect the experimental results.\u003cbr\u003e\u003cbr\u003e8. Please wear a lab coat and latex gloves for proper protection during the experiment, especially when testing blood or other body fluid samples. Please follow the National Biological Laboratory Safety Protection Regulations.\u003cbr\u003e\u003cbr\u003e9. Components of the kit from different batches cannot be mixed (except for wash buffer and reaction stop solution).\u003cbr\u003e\u003cbr\u003e10. The enzyme label strips in the kit are detachable. Please use them in batches according to experimental needs.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIII. Operation Procedure\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Before beginning the experiment, all reagents should be equilibrated to room temperature and all reagents should be prepared in advance. When diluting reagents or samples, mix thoroughly and try to avoid foaming during mixing. If the sample concentration is too high, dilute it with sample diluent to bring the sample within the detection range of the kit.\u003cbr\u003e\u003cbr\u003e2. Add 100 μL of the standard or sample to be tested (if the sample needs to be diluted, refer to the sample dilution guidelines for dilution methods). Be careful not to create bubbles. Add the sample to the bottom of the plate well, avoiding contact with the well walls. Gently shake to mix. Cover the plate with a film or film and incubate at 37°C for 80 minutes. To ensure the validity of the experimental results, use a fresh standard solution for each experiment.\u003cbr\u003e\u003cbr\u003e3. Discard the liquid in the wells, spin dry, and wash the plate three times. Wash each well with 200 μL of wash buffer, soak for 1-2 minutes, and spin off the liquid in the plate (or wash the plate using a plate washer). After the final wash, pat the plate dry on absorbent paper.\u003cbr\u003e\u003cbr\u003e4. Add 100 μL of biotinylated antibody working solution to each well (can be prepared 15 minutes in advance), cover the plate with film, and incubate at 37°C for 50 minutes.\u003cbr\u003e\u003cbr\u003e5. Discard the liquid in the wells and wash the plate three times. Wash each well with 200 μL of wash buffer, soaking for 1-2 minutes. Discard the liquid in the plate (or wash the plate using a plate washer). After the final wash, pat the plate dry on absorbent paper.\u003cbr\u003e\u003cbr\u003e6. Add 100 μL of enzyme conjugate working solution to each well (can be prepared 15 minutes in advance) and incubate at 37°C for 50 minutes.\u003cbr\u003e\u003cbr\u003e7. Discard the liquid in the wells and wash the plate five times. Wash each well with 200 μL of wash buffer, soak for 1-2 minutes, and shake off any remaining liquid from the plate (or wash using a plate washer). After the final wash, pat the plate dry on absorbent paper.\u003cbr\u003e\u003cbr\u003e8. Add 90 μL of TMB chromogenic substrate solution to each well and incubate at 37°C in the dark for 20 minutes (shorten or extend the time depending on the color development, but do not exceed 30 minutes).\u003cbr\u003e\u003cbr\u003e9. Add 50 μL of stop solution to each well to terminate the reaction (the blue color will immediately turn yellow). The stop solution should be added in the same order as the color developer. To ensure accurate results, add the stop solution as soon as possible after the substrate reaction time expires.\u003cbr\u003e\u003cbr\u003e10. Immediately measure the optical density (OD) of each well using a microplate reader at a wavelength of 450 nm. The instrument should be preheated and the assay program set before use.\u003cbr\u003e\u003cbr\u003eCalculation of Results\u003cbr\u003e1. The OD value of the blank well should be subtracted from the OD value of each standard and sample. If replicate wells are used, the average value should be used for calculation.\u003cbr\u003e\u003cbr\u003e2. For ease of calculation, although concentration is the independent variable and OD value is the dependent variable, the graphs use the OD value of the standard as the horizontal axis (X-axis) and the concentration of the standard as the vertical axis (Y-axis). To ensure intuitiveness of the experimental results, the graphs present raw data rather than logarithmic values. Due to differences in experimental operating conditions (such as operator, pipetting technique, plate washing technique, and temperature), the OD values of the standard curve will vary. The provided standard curve is for reference only; experimenters should establish their own standard curve. The sample concentration can be calculated from the OD value of the used sample on the standard curve. This value is then multiplied by the dilution factor to obtain the actual sample concentration. It is recommended to use professional curve drawing software, such as curve expert.\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration (\u003c\/strong\u003e\u003cstrong\u003eng\/mL) \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e\u003cstrong\u003eOD \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e5000\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e2.268\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e2.171\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e2500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e1.72\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e1.623\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e1250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e1.174\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e1.077\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e625\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.892\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.795\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e312.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.507\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.41\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e156.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.305\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.208\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e78.13\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.261\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e0.164\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e0.097\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240801\/2592b13957624e05a20c9cc415ab6e5e.png\" alt=\"\" width=\"710\" height=\"406\"\u003e\u003c\/p\u003e\n\u003cp\u003e                                                                           Note: This figure is for reference only.\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003ePrecision\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eIntra-plate precision (within-assay precision): CV% \u0026lt;8%\u003cbr\u003e\u003cbr\u003eThree samples of known concentrations were tested 20 times on a single ELISA plate to assess intra-plate precision.\u003cbr\u003e\u003cbr\u003eInter-plate precision (between-assay precision): CV% \u0026lt;10%\u003cbr\u003e\u003cbr\u003eThree samples of known concentrations were tested 40 times on three different ELISA plates to assess inter-plate precision.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eRecovery experiments were performed by spiking different samples with known concentrations of rabbit MYO to determine the recovery range and average recovery.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"margin: 0px auto; height: 283px; width: 49.0311%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp style=\"text-align: center;\"\u003eSample Type\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003eRecovery Range\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003eAverage Recovery\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003eSerum(n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003e85-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003e92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003eEDTA plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003e83-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003e90%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003eheparin plasma(n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp\u003e80-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%;\" width=\"33.3200%\"\u003e\u003cp style=\"text-align: center;\"\u003e86%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLinearity\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eThe samples spiked with rabbit MYO were diluted 2-fold, 4-fold, 8-fold, and 16-fold to perform recovery experiments and obtain the recovery rate range.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"margin: 0px auto; height: 283px; width: 57.1188%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.7378%;\"\u003e\u003cp style=\"text-align: center;\"\u003eSample type\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 12.8609%;\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 29.7378%;\"\u003e\u003cp\u003eSerum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e88-104%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e86-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e79-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.8609%;\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 29.7378%;\"\u003e\u003cp\u003eEDTA plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e87-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.8209%;\"\u003e\u003cp\u003e82-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.8609%;\"\u003e\u003cp\u003e82-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 29.7378%;\"\u003e\u003cp\u003eheparin plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e79-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 14.8209%;\"\u003e\u003cp\u003e93-106%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.8609%;\"\u003e\u003cp style=\"text-align: center;\"\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit utilizes a sandwich assay. A specific anti-rabbit MYO antibody is coated in a 96-well microplate. Rabbit MYO standards or samples are added to the wells, allowing the rabbit MYO protein in the standard or sample to bind to the anti-rabbit MYO antibody immobilized on the microplate. Biotinylated anti-rabbit MYO antibody is then added. Unbound biotinylated antibody is washed away, and HRP-conjugated streptavidin is added. After thorough washing again, TMB substrate is added for color development. TMB converts to blue under peroxidase catalysis and to the final yellow under the action of acid. The intensity of the color is positively correlated with the amount of rabbit MYO protein in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader, and the sample concentration is calculated by plotting a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePVALB; MB；Rabbit MYO(Myoglobin) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCan detect recombinant or native rabbit MYO and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%; text-align: center;\" width=\"27.6200%\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%; text-align: center;\" width=\"25.0600%\"\u003e\u003cp\u003e\u003cstrong\u003eSave conditions\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eELISA plate (removable)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e12 strips x 8 holes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eFreeze-dried standard\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eStandard \u0026amp;Sample Dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eConcentrated Biotinylated Antibody (100×)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eBiotinylated Antibody Dilution Buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eConcentrated HRP Enzyme Conjugate (100×)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eEnzyme conjugate diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eConcentrated detergent (25×)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eChromogenic substrate solution (TMB)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C (protect from light)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 47.3%;\" width=\"47.3000%\"\u003e\u003cp\u003eReaction stop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%;\" width=\"27.6200%\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 47.3%; text-align: center;\" width=\"47.3000%\"\u003e\u003cp\u003eSealing film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 27.62%; text-align: center;\" width=\"27.6200%\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.06%;\" width=\"25.0600%\"\u003e\u003cp style=\"text-align: center;\"\u003eNormal temperature\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. If the entire kit is stored at -20°C, please place the kit at 4°C the night before the experiment.\u003c\/p\u003e\n\u003cp\u003e2. Salt precipitation may occur when the concentrated wash solution is stored at low temperatures. When diluting, warm it in a water bath to help dissolve it.\u003c\/p\u003e\n\u003cp\u003e3. A small amount of water-like substance may be present in the wells of a newly opened ELISA plate. This is normal and will not affect the experimental results.\u003c\/p\u003e\n\u003cp\u003e4. This kit is for laboratory research and development use only and is not intended for use on humans or animals.\u003c\/p\u003e\n\u003cp\u003e5. Reagents should be treated as hazardous substances and should be handled with care and disposed of properly.\u003c\/p\u003e\n\u003cp\u003e6. Always wear gloves, lab coats, and protective glasses to avoid contact between skin and eyes with the stop solution and TMB. If contact occurs, rinse thoroughly with water.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 78.13-5000 pg\/mL; Sensitivity: 28 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678541357131,"sku":"abs554103-96T","price":413.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vancomycin-elisa-kit","title":"Vancomycin ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003e1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e4. Cell lysis solution: Gently wash adherent cells with pre-cooled PBS, then digest with trypsin, and collect the cells after centrifugation at 1000×g for 5 minutes; suspended cells can be directly collected by centrifugation. Wash the collected cells 3 times with pre-cooled PBS, add 150-200uL PBS for every 1×10^6 cells to resuspend (it is recommended to add protease inhibitors to PBS; if the content is very low, the PBS volume can be appropriately reduced) and break the cells by repeated freezing and thawing or ultrasound. Centrifuge the extract at 2-8℃, 1500×g for 10 minutes, and take the supernatant for detection.\u003cbr\u003e5. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test.\u003cbr\u003e\u003cbr\u003ePre-test preparation:\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration 1000 ng\/mL). Then dilute to the following concentrations: 1000 ng\/mL, 500 ng\/mL, 250 ng\/mL, 125 ng\/mL, 62.5 ng\/mL, 31.25 ng\/mL, 15.625 ng\/mL, and 0 ng\/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 1000ng\/mL standard working solution into the first EP tube and mix thoroughly to make a 500ng\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day.\u003cbr\u003e4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day.\u003cbr\u003e5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.)\u003cbr\u003e3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes.\u003cbr\u003e5. Wash: Discard the liquid and wash the plate five times as in step 3.\u003cbr\u003e6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003eCalculation of experimental results:\u003cbr\u003eResult evaluation:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-china\/20250312\/236bc30b9882438bb8f2bcbf2cb803ad.png\" alt=\"\" width=\"512\" height=\"356\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal vancomycin antigen. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the universal vancomycin content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVancomycin ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompetition Law\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate  Biotin- Antibody ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e60uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVancomycin is a glycopeptide antibiotic used to treat serious infections that have failed all other antibiotics. It acts by inhibiting the second phase of cell wall synthesis in susceptible bacteria, altering cell membrane permeability and selectively inhibiting ribonucleic acid synthesis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.62-1000ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678541946955,"sku":"abs554145-96T","price":511.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cat-il-6-elisa-kit","title":"Cat IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect the specimen using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test it.\u003cbr\u003eAlternatively, store it at -20°C or -80°C.\u003cbr\u003eHowever, repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eRemove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250625\/0dd53169259e409c8acaf4a6985c432e.png\" alt=\"\" width=\"600\" height=\"354\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable concentration was less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Cat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with feline interleukin-6 (IL-6) capture antibodies. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The intensity of the color is positively correlated with the amount of feline interleukin-6 (IL-6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of feline interleukin 6 (IL-6) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"270\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"139\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"139\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"270\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 500, 250, 125, 62.5, 31.25, and 15.625 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly loaded. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 2-8°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.625 pg\/mL – 500 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678542831691,"sku":"abs554479-96T","price":381.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rabbit-tnf-a-elisa-kit","title":"Rabbit TNF-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch2\u003e\u003cstrong\u003eSample Processing and Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSerum:\u003c\/strong\u003e Place whole blood specimens collected in serum separator tubes at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant and store at -20°C or -80°C.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003ePlasma:\u003c\/strong\u003e Collect specimens using EDTA or heparin as an anticoagulant.\u003cbr\u003eWithin 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.\u003cbr\u003eRemove the supernatant and test.\u003cbr\u003eAlternatively, store at -20°C or -80°C.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eTissue Homogenization:\u003c\/strong\u003e Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).\u003cbr\u003eWeigh the tissue and mince it.\u003cbr\u003eAdd the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.\u003cbr\u003eThe specific volume can be adjusted according to experimental needs and recorded.\u003cbr\u003eIt is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.\u003cbr\u003eTo further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.\u003cbr\u003eFinally, centrifuge the homogenate at 5000×g for 5-10 minutes.\u003cbr\u003eRemove the supernatant for analysis.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eCell culture supernatant or other biological specimens:\u003c\/strong\u003e Centrifuge at 1000×g for 20 minutes.\u003cbr\u003eRemove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003eNote: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003cbr\u003eAfter removing the reagent kit from the refrigerator, allow it to equilibrate to room temperature before use.\u003cbr\u003eDilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1.\u003c\/strong\u003e\u003cbr\u003eAfter equilibration at room temperature for 20 minutes, remove the desired strips from the aluminum foil bag.\u003cbr\u003eSeal the remaining strips in a ziplock bag and return them to 4°C.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e2.\u003c\/strong\u003e\u003cbr\u003eSet up standard wells and sample wells.\u003cbr\u003eAdd 50 μL of standard solution of varying concentrations to each standard well.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cbr\u003eAdd 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.\u003cbr\u003eSeal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e5.\u003c\/strong\u003e\u003cbr\u003eDiscard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).\u003cbr\u003eLet stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.\u003cbr\u003eRepeat this process five times (a plate washer can also be used).\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e6.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e7.\u003c\/strong\u003e\u003cbr\u003eAdd 50 μL of stop solution to each well.\u003cbr\u003eWithin 15 minutes, measure the OD value of each well at a wavelength of 450 nm.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eCalculation of Experimental Results:\u003c\/strong\u003e\u003cbr\u003eUsing the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, draw a standard curve on graph paper or using relevant software.\u003cbr\u003eObtain a linear regression equation.\u003cbr\u003eSubstitute the OD value of the sample into the equation to calculate the sample concentration.\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250220\/8a3fb3be411346a2b5d4177b6058ca08.png\" alt=\"\" width=\"716\" height=\"422\"\u003e\u003cbr\u003eStandard Curve\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit utilizes a double-antibody, one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are sequentially added to microwells pre-coated with a rabbit tumor necrosis factor α (TNF-α) capture antibody. The sample is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under peroxidase catalysis and to yellow under acid. The color intensity is positively correlated with the amount of rabbit tumor necrosis factor α (TNF-α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRabbit TNF-a(Tumor Necrosis Factor Alpha) ELISA Kit；TNF Alpha\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for in vitro quantitative detection of rabbit tumor necrosis factor α (TNF-α) content in serum, plasma, tissue homogenate and related liquid samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"260\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"186\"\u003e\u003cp\u003e\u003cstrong\u003e96 \u003c\/strong\u003e\u003cstrong\u003eT\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"222\"\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eMicrowell enzyme plate\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e12 wells×8 strips\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStandard product\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e0.3mL*6 tubes\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSample diluent\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eDetection antibody-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003e20× wash buffer\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eDilution according to the instructions\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eSubstrate B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"260\"\u003e\u003cp\u003eStop solution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"186\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"260\"\u003e\u003cp\u003eSeal film\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"186\"\u003e\u003cp\u003e2 sheets\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"222\"\u003e\u003cp style=\"text-align: center;\"\u003eNone\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eRemarks\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e1. The concentrations of the standard solutions are: 320, 160, 80, 40, 20, and 10 pg\/mL.\u003c\/p\u003e\n\u003cp\u003e2. After testing a large number of normal specimens, the normal concentrations of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly loaded. If some sample values exceed the maximum standard concentration, the specimens can be appropriately diluted with sample diluent before the experiment.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.\u003c\/p\u003e\n\u003cp\u003e2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.\u003c\/p\u003e\n\u003cp\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.\u003c\/p\u003e\n\u003cp\u003e4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.\u003c\/p\u003e\n\u003cp\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.\u003c\/p\u003e\n\u003cp\u003e6. Avoid direct exposure to strong light during storage and incubation.\u003c\/p\u003e\n\u003cp\u003e7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.\u003c\/p\u003e\n\u003cp\u003e8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.\u003c\/p\u003e\n\u003cp\u003e Any bleaching component will destroy the biological activity of the reagents in the kit. \u003c\/p\u003e\n\u003cp\u003e9. Do not use expired products. \u003c\/p\u003e\n\u003cp\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened test kit, stored at 4°C, has a shelf life of 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 pg\/mL – 320 pg\/mL; Sensitivity: Minimum detection concentration is less than 1.0 pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678543061067,"sku":"abs554126-96T","price":413.0,"currency_code":"USD","in_stock":true}]},{"product_id":"simian-il-6-elisa-kit","title":"Simian IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e 1. \u003c\/strong\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect. The blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes. deposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect. Anticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples. deposit -20°C Or -80°C Storage, avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded. It is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice; In order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times). Finally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed. Take the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save. Avoid repeated freezing and thawing. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e attention \u003c\/strong\u003e\u003cstrong\u003e ： \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation. Hemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing. Keep the sample at room temperature prior to the experiment. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. If the concentration of the detected substance in your sample is higher than the highest value of the standard, please make an appropriate dilution according to the actual situation (it is recommended to do a pre-experiment first to determine the dilution factor). \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Two, \u003c\/strong\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 1000 pg\/mL ）。 Thereafter, double dilution is carried out to 1000 pg\/mL ， 500 pg\/mL ， 250 pg\/mL ， 125 pg\/mL ， 62.5 pg\/mL ， 31.25 pg\/mL ， 15.63 pg\/mL Standard dilution ( 0 pg\/mL ) is a blank hole. Configure the standard according to the amount you need for later use. The configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250407\/8aaf9eb6c60e4056ae8b91b7e73b7b05.png\" alt=\"\" width=\"600\" height=\"227\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day. Dilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。 Before use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day. Dilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e attention \u003c\/strong\u003e\u003cstrong\u003e ： \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Please make sure that all components are dissolved and mixed before use of the kit. If the reconstituted standard is not used, please discard it. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Concentrated biotinylated antibody, the volume of concentrated enzyme conjugate is small, may be dispersed in various parts of the tube during transportation, please 1000×g Centrifugation 1 Minutes to allow the liquid of the tube wall or cap to deposit to the bottom of the tube. Pipette carefully before use 4-5 The solution was mixed once. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40°C ）。 The wash liquid should be at room temperature when used. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Adding samples should be quick, and it is best to control each sample adding within 10 Within minutes, in order to ensure the accuracy of the experiment, it is recommended to use a double hole. When pipetting reagents, a consistent sequence of addition is maintained from well to well,   This will ensure the same hatch time for all holes. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. During the washing process, the washing液 remaining in the reaction hole should be patted dry on absorbent paper, and do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Color developer TMB Direct exposure to bright light should be avoided during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. The test tubes and reagents used in the experiment are disposable, and it is strictly forbidden to reuse them, otherwise it will affect the experimental results. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e8. During the experiment, please wear a laboratory coat and latex gloves for protection, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e9. The kit components of different lot numbers cannot be mixed (except wash solution and reaction stop solution). \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e10. The enzyme labeling strips in the kit are detachable plates, please use them in batches according to the experimental requirements. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Three, \u003c\/strong\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance. When reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well. If the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method). Be careful not to have bubbles. When adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate. Cover or coating, 37°C incubation 80 Minutes. To ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times. For each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e5. Discard the liquid in the well and wash the plate 3 Times. For each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e7. Discard the liquid in the well and wash the plate 5 Times. For each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer). After the last wash was complete, the plate was pat dry on absorbent paper. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes). \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time). The sequence of addition of the terminating solution should be the same as that of the developer as possible. In order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value). The instrument should be preheated before use, and the testing program should be set up. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e1. Of each standard and sample OD Value should be subtracted from the blank孔 OD Value. If a double hole is set, the average value should be taken for calculation. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis). At the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values. Due to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary. The standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment. Spent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample. It is recommended to use professional curve drawing software such as curve expert 。 \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"236\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eConcentration \u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003epg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"160\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"160\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e1000\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.978\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.883\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.535\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.251\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.156\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e125\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.768\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.673\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e62.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.583\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.488\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e31.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.331\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.236\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e15.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.235\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.14\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"236\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"160\"\u003e\u003cp\u003e0.095\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp style=\"text-align: center;\"\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241105\/df82f29bcd1e4cfc9beb4f9044a40e1e.png\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e Note: This picture is for reference only \u003c\/div\u003e\n\u003cbr\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to assess the accuracy between the assay plates. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e Add monkeys at known concentrations to different samples  IL6 , do the recovery experiment, get the recovery range and average recovery rate \u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e92-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e81-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"189\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp style=\"text-align: center;\"\u003e99%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e There will be monkeys added  IL6 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range \u003c\/p\u003e\n\u003ctable style=\"margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"158\"\u003e\u003cp style=\"text-align: center;\"\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e95-103%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e80-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e87-98%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-92%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e87-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e89-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"158\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e89-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e85-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"102\"\u003e\u003cp\u003e79-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp style=\"text-align: center;\"\u003e85-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSimian\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IL-6 antibody was coated in a 96-well microplate, and monkey IL-6 standard or sample was added to the microwells, so that monkey IL-6 protein in the standard or monkey IL-6 protein in the sample was bound to the anti-monkey IL-6 antibody solid on the microplate, then biotinylated anti-monkey IL-6 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL-6 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma\/Plasmacytoma Growth Factor; Hepatocyte\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant or native monkey IL-6 can be detected and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0px auto; height: 200px; width: 60%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 42.6334%; text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 20.348%;\"\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 27.7717%;\"\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Enzyme labeled plate (detachable) \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e8-Well × 12 Strips \u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Lyophilized Standard \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Standard \u0026amp; Sample dilution \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Biotinylated antibody dilution \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Enzyme conjugate dilution \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Concentrated wash ( 25× ） \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e TMB Substrate Solution \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C( Protected from light) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"text-align: center;\"\u003e\n\u003ctd style=\"width: 42.6334%;\"\u003e Reaction stop solution \u003c\/td\u003e\n\u003ctd style=\"width: 20.348%;\"\u003e6 mL\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%;\"\u003e4°C\/-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 42.6334%;\"\u003e Sealing film \u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 20.348%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 27.7717%; text-align: center;\"\u003e normal temperature \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. If the entire kit is stored at-20 °C, please place the kit at 4 °C the night before the experiment.\u003c\/p\u003e\n\u003cp\u003e2. Salt precipitation will occur when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve.\u003c\/p\u003e\n\u003cp\u003e3. The newly opened wells of the enzyme labeled plate may contain a little water-like substance, which is a normal phenomenon and will not have any impact on the experimental results.\u003c\/p\u003e\n\u003cp\u003e4. This kit is intended for laboratory research and development use only, not for human or animal use.\u003c\/p\u003e\n\u003cp\u003e5. Reagents should be treated as hazardous substances and should be handled carefully and properly disposed of.\u003c\/p\u003e\n\u003cp\u003e6. Gloves, lab coats and protective glasses should always be worn to avoid skin and eye contact with terminating solution and TMB. In case of accidental contact, please wash thoroughly with water.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL; Sensitivity: 5.8 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678619607115,"sku":"abs554110-96T","price":429.0,"currency_code":"USD","in_stock":true}]},{"product_id":"simian-il-2elisa-kit","title":"Simian IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e 1. \u003c\/strong\u003e\u003cstrong\u003e Sample collection preparation and preservation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Serum: Whole blood sample placed at room temperature 2 Hour or 4°C Overnight after 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003cbr\u003eThe blood collection tubes shall be disposable non-pyrogenic, non-endotoxin tubes.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e2. Plasma: Sample after collection 30 Within minutes 2-8°C 、 1000×g Centrifugation 15 Minutes, take the supernatant to detect.\u003cbr\u003eAnticoagulants recommended EDTA-Na\u003csub\u003e2\u003c\/sub\u003e , avoid using hemolytic, hyperlipidemic samples.\u003cbr\u003edeposit -20°C Or -80°C Storage, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e3. Tissue homogenate: Take an appropriate amount of tissue block and add it to the pre-cooled PBS （ 0.01M ， pH7.0-7.2 ) to remove blood (lysed red blood cells in the homogenate will affect the measurement result), cut the tissue into pieces after weighing, and then mix it with the corresponding volume of PBS (generally according to 1:9 The mass-to-volume ratio, the specific volume can be appropriately adjusted according to the needs of the experiment, and recorded.\u003cbr\u003eIt is recommended to PBS Adding a protease inhibitor) into a glass homogenizer and fully grinding on ice;\u003cbr\u003eIn order to further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated 2 Times).\u003cbr\u003eFinally, the prepared homogenate is mixed in 5000×g Centrifugation 5-10 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e4. Cell culture supernatant: Take the cell supernatant from 1000×g Centrifugation 20 Minutes, impurities and cell debris were removed.\u003cbr\u003eTake the supernatant to detect and place it in -20°C Or -80°C Store, but repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e5. Urine: Please collect the first urine in the morning (mid-section urine), or  24 Hourly urine, 2000×g  Centrifugation  15  The supernatant was collected after minutes and the sample was saved   At -20°C And repeated freezing and thawing should be avoided.\u003c\/p\u003e\n\u003cp\u003e6. Saliva: A sample is collected with a saliva sample collection tube, and then  2-8°C, 1000×g  Centrifugation  15  Minutes, take the supernatant to detect, or sub-package -20°C  Save.\u003cbr\u003eAvoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp\u003e7. Other biological samples: Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e attention \u003c\/strong\u003e\u003cstrong\u003e ： \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The sample should be clear and transparent, and the suspended solids should be removed by centrifugation.\u003cbr\u003eHemolysis of the sample will affect the results, so hemolyzed samples should not be used.\u003c\/p\u003e\n\u003cp\u003e2. After sample collection, if 1 Testing within weeks can be stored at 4°C , if it cannot be detected in time, please pack it according to the one-time usage amount and freeze it in  -20°C （ 1 Within months), or -80°C （ 3-6 Test within a month) to avoid repeated freezing and thawing.\u003cbr\u003eKeep the sample at room temperature prior to the experiment.\u003c\/p\u003e\n\u003cp\u003e3. If the concentration of the detected substance in your sample is higher than the highest value of the standard, please make an appropriate dilution according to the actual situation (it is recommended to do a pre-experiment first to determine the dilution factor).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Two, \u003c\/strong\u003e\u003cstrong\u003e Preparation for testing \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please advance 30 Minutes remove the kit from the refrigerator and equilibrate to room temperature.\u003c\/p\u003e\n\u003cp\u003e2. Use double distilled water 25× The concentrated wash liquid is diluted to 1× Working fluid, put back unused 4°C.\u003c\/p\u003e\n\u003cp\u003e3. Standard: Add standard \u0026amp; Sample Universal Diluent 1.0 mL Into the lyophilized standard, screw the tube cap tightly and let stand 10 Minutes, and after it is fully dissolved, gently mix (concentration of 1000 pg\/mL ）。\u003cbr\u003eThereafter, double dilution is carried out to 1000 pg\/mL ， 500 pg\/mL ， 250 pg\/mL ， 125 pg\/mL ， 62.5 pg\/mL ， 31.25 pg\/mL ， 15.63 pg\/mL Standard dilution ( 0 pg\/mL ) is a blank hole.\u003cbr\u003eConfigure the standard according to the amount you need for later use.\u003cbr\u003eThe configured standards are recommended in 15 Add the sample within minutes, and it is not recommended to leave it for too long.\u003cbr\u003e\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250407\/04aaddf3260741adb48bbcfa0bbd66d4.png\" alt=\"\" width=\"600\" height=\"234\"\u003e\u003c\/p\u003e\n\u003cp\u003e4. Biotinylated antibody working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ), before use 15 Min, concentrated biotinylated antibody was diluted with biotinylated antibody diluent ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL Concentrated biotinylated antibody is added to 99 μL In the biotinylated antibody dilution, mix well with a pipette.\u003c\/p\u003e\n\u003cp\u003e5. Enzyme conjugate working solution: calculate the dosage required for the current experiment before the experiment (according to 100 μL\/ Hole meter, should be configured more in actual configuration 100-200 μL ）。\u003cbr\u003eBefore use 15 Minutes, dilute and concentrate with enzyme conjugate diluent HRP Enzyme conjugate ( 1:100 ) into working concentration, use on the same day.\u003cbr\u003eDilution principle 1 μL The concentrated enzyme conjugate is added to 99 μL The enzyme conjugate dilution was mixed with a pipette.\u003c\/p\u003e\n\u003cp\u003e6.TMB Substrate —— Pipette the desired dose of solution and do not pour the residual solution back into the reagent vial again.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e attention \u003c\/strong\u003e\u003cstrong\u003e ： \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Please make sure that all components are dissolved and mixed before use of the kit.\u003cbr\u003eIf the reconstituted standard is not used, please discard it.\u003c\/p\u003e\n\u003cp\u003e2. Concentrated biotinylated antibody, the volume of concentrated enzyme conjugate is small, may be dispersed in various parts of the tube during transportation, please 1000×g Centrifugation 1 Minutes to allow the liquid of the tube wall or cap to deposit to the bottom of the tube.\u003cbr\u003ePipette carefully before use 4-5 The solution was mixed once.\u003cbr\u003eStandard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.\u003c\/p\u003e\n\u003cp\u003e3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon.\u003cbr\u003eThe crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40°C ）。\u003cbr\u003eThe wash liquid should be at room temperature when used.\u003c\/p\u003e\n\u003cp\u003e4. Adding samples should be quick, and it is best to control each sample adding within 10 Within minutes, in order to ensure the accuracy of the experiment, it is recommended to use a double hole.\u003cbr\u003eWhen pipetting reagents, a consistent sequence of addition is maintained from well to well,   This will ensure the same hatch time for all holes.\u003c\/p\u003e\n\u003cp\u003e5. During the washing process, the washing液 remaining in the reaction hole should be patted dry on absorbent paper, and do not put the filter paper directly into the reaction hole to absorb water.\u003cbr\u003eBefore reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e6. Color developer TMB Direct exposure to bright light should be avoided during storage and use.\u003cbr\u003eAfter adding the substrate, pay attention to the color change in the reaction well.\u003cbr\u003eIf the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.\u003c\/p\u003e\n\u003cp\u003e7. The test tubes and reagents used in the experiment are disposable, and it is strictly forbidden to reuse them, otherwise it will affect the experimental results.\u003c\/p\u003e\n\u003cp\u003e8. During the experiment, please wear a laboratory coat and latex gloves for protection, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.\u003c\/p\u003e\n\u003cp\u003e9. The kit components of different lot numbers cannot be mixed (except wash solution and reaction stop solution).\u003c\/p\u003e\n\u003cp\u003e10. The enzyme labeling strips in the kit are detachable plates, please use them in batches according to the experimental requirements.\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e Three, \u003c\/strong\u003e\u003cstrong\u003e Operation steps \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Before the start of the experiment, each reagent should be balanced to room temperature, and all reagents should be configured in advance.\u003cbr\u003eWhen reagents or samples are diluted, they should be mixed well, and blistering should be avoided as much as possible when mixing well.\u003cbr\u003eIf the sample concentration is too high, dilute with a sample diluent to bring the sample within the range of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Add standard or sample to be tested 100 μL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method).\u003cbr\u003eBe careful not to have bubbles.\u003cbr\u003eWhen adding the sample, add the sample to the bottom of the well of the enzyme label plate, try not to touch the well wall, gently shake and mix well, and add the enzyme label plate.\u003cbr\u003eCover or coating, 37°C incubation 80 Minutes.\u003cbr\u003eTo ensure the validity of the experimental results, please use a new standard solution for each experiment.\u003c\/p\u003e\n\u003cp\u003e3. Discard the liquid in the hole, spin dry, wash the plate 3 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e4. Add biotin antibody working solution per well 100μL (can be advanced 15 Preparation in minutes), the enzyme labeled plate is coated, 37°C incubation 50 Minutes.\u003c\/p\u003e\n\u003cp\u003e5. Discard the liquid in the well and wash the plate 3 Times.\u003cbr\u003eFor each well 200μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e6. Add enzyme conjugate working solution per well 100 μL (can be advanced 15 Minute preparation), 37°C incubation 50 Minutes.    \u003c\/p\u003e\n\u003cp\u003e7. Discard the liquid in the well and wash the plate 5 Times.\u003cbr\u003eFor each well 200 μL Washing with washing solution, soaking 1-2 Minutes, shake off the liquid in the labeled plate (or wash the plate with a plate washer).\u003cbr\u003eAfter the last wash was complete, the plate was pat dry on absorbent paper.\u003c\/p\u003e\n\u003cp\u003e8. Add per well TMB Chromogenic substrate solution 90 μL ， 37°C Incubate in the dark 20 Minutes (shortened or extended as appropriate according to the actual color development, but not exceeding 30 Minutes).\u003c\/p\u003e\n\u003cp\u003e9. Add stop solution to each well 50 μL , terminate the reaction (blue immediately turns yellow at this time).\u003cbr\u003eThe sequence of addition of the terminating solution should be the same as that of the developer as possible.\u003cbr\u003eIn order to ensure the accuracy of the experimental results, the termination solution should be added as soon as possible after the substrate reaction time expires.\u003c\/p\u003e\n\u003cp\u003e10. Immediately use a microplate reader in 450 nm The optical density values of each well were measured at the wavelength ( OD Value).\u003cbr\u003eThe instrument should be preheated before use, and the testing program should be set up.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Results Calculation \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Of each standard and sample OD Value should be subtracted from the blank孔 OD Value.\u003cbr\u003eIf a double hole is set, the average value should be taken for calculation.\u003c\/p\u003e\n\u003cp\u003e2. For ease of calculation, although the concentration is an independent variable and OD The value is the dependent variable, and we still use the standard when drawing OD Values as abscissa ( X Axis), the concentration of the standard is the ordinate ( Y Axis).\u003cbr\u003eAt the same time, for the intuition of the test results, the figure provides raw data instead of logarithmic values.\u003cbr\u003eDue to the different experimental operating conditions (such as operator, pipetting technology, plate washing technology and temperature conditions, etc.), the standard curve OD Values will vary.\u003cbr\u003eThe standard curve provided is for reference only, and the experimenter needs to establish the standard curve according to his own experiment.\u003cbr\u003eSpent sample OD The value can be calculated on the standard curve to calculate the sample concentration and multiply it by the dilution factor, which is the actual concentration of the sample.\u003cbr\u003eIt is recommended to use professional curve drawing software such as curve expert.\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; height: 20px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e\u003cstrong\u003eConcentration \u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003epg\/mL\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e\u003cstrong\u003eOD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e\u003cstrong\u003eCorrected OD\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e1000\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e2.126\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e2.018\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e500\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.602\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.494\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e250\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.123\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e1.015\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e125\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.872\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.764\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e62.5\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.514\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.406\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e31.25\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.325\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.217\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e15.63\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.256\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.148\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"236\"\u003e\u003cp\u003e0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.108\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"160\"\u003e\u003cp\u003e0.000\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241105\/6e6d26c3ff82437eab96deb74104f4f3.png\" alt=\"\" width=\"550\" height=\"328\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e Note: This picture is for reference only \u003c\/div\u003e\n\u003cbr\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Precision \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e Intraplate precision ( Precision within the assay ):CV%\u0026lt;8%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 1 Test on enzyme label plates 20 Times to evaluate the precision in the assay plate.\u003c\/p\u003e\n\u003cp\u003e Inter-plate precision ( Measure inter-plate precision ):CV%\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003e Three samples with known concentrations were respectively in 3 Tested on different enzyme plates 40 Times to assess the precision between assays.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e Recovery \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e Add monkeys at known concentrations to different samples  IL2 , do the recovery experiment, get the recovery range and average recovery rate\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; height: 10px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e Recovery Range \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e Average recovery \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e85-97%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e91%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e81-93%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e87%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e82-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"189\"\u003e\u003cp\u003e88%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\u003cstrong\u003e linear \u003c\/strong\u003e\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e There will be monkeys added  IL2 The samples were diluted separately 2 Times, 4 Times, 8 Times, 16 Double the recovery experiment to obtain the recovery rate range\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; height: 10px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003e Sample Type \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e1:2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e1:4\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e1:8\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e1:16\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003e Serum (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e95-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e93-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e81-89%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003eEDTA  Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e86-94%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e89-99%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e85-95%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd width=\"158\"\u003e\u003cp\u003eheparin Plasma (n=5)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e87-96%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e92-105%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e89-102%\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"102\"\u003e\u003cp\u003e92-101%\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSimian\/Monkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. The specific anti-monkey IL-2 antibody was coated in a 96-well microplate, and the monkey IL-2 standard or sample was added to the microwells respectively, so that the monkey IL-2 protein in the standard or the monkey IL-2 protein in the sample was bound to the anti-monkey IL-2 antibody solid on the microplate, then biotinylated anti-monkey IL-2 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and then TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL-2 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMonkey IL2 ELISA Kit; Monkey Interleukin-2 ELISA kit; Monkey TCGF ELISA Kit; Monkey T-Cell Growth Factor ELISA Kit; Monkey Aldesleukin ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant or native monkey IL-2 can be detected and does not cross-react with other related proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cdiv style=\"text-align: center;\"\u003e\u003ctable style=\"border-collapse: collapse; width: 62.755%; height: 50px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e\u003cstrong\u003e96T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e\u003cstrong\u003e Preservation conditions \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Enzyme labeled plate (detachable) \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e8-Well × 12 Strips \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Lyophilized Standard \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Standard \u0026amp; Sample dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Biotinylated antibody dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e120 μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Enzyme conjugate dilution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e12 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Concentrated wash ( 25× ） \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e20 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e TMB Substrate Solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e10 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C( Protected from light) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Reaction stop solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e6 mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e4°C\/-20°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 44.2321%;\"\u003e\u003cp\u003e Sealing film \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 22.3895%;\"\u003e\u003cp\u003e2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8963%;\"\u003e\u003cp\u003e normal temperature \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. If the entire kit is stored at-20 °C, please place the kit at 4 °C the night before the experiment.\u003c\/p\u003e\n\u003cp\u003e2. Salt precipitation will occur when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve.\u003c\/p\u003e\n\u003cp\u003e3. The newly opened wells of the enzyme labeled plate may contain a little water-like substance, which is a normal phenomenon and will not have any impact on the experimental results.\u003c\/p\u003e\n\u003cp\u003e4. This kit is intended for laboratory research and development use only, not for human or animal use.\u003c\/p\u003e\n\u003cp\u003e5. Reagents should be treated as hazardous substances and should be handled carefully and properly disposed of.\u003c\/p\u003e\n\u003cp\u003e6. Gloves, lab coats and protective glasses should always be worn to avoid skin and eye contact with terminating solution and TMB. In case of accidental contact, please wash thoroughly with water.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4 °C, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL; Sensitivity: 5.5 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678620590155,"sku":"abs554109-96T","price":429.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tobramycin-elisa-kit","title":"Tobramycin ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental equipment required for the experiment:\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (450nm)\u003cbr\u003e2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL\u003cbr\u003e3. 37℃ constant temperature box\u003cbr\u003e4. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSample processing and requirements:\u003c\/strong\u003e\u003cbr\u003e1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing.\u003cbr\u003e3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed.\u003cbr\u003e4. Cell lysis solution: Gently wash adherent cells with pre-cooled PBS, then digest with trypsin, and collect the cells after centrifugation at 1000×g for 5 minutes; suspended cells can be directly collected by centrifugation. Wash the collected cells 3 times with pre-cooled PBS, add 150-200uL PBS for every 1×10^6 cells to resuspend (it is recommended to add protease inhibitors to PBS; if the content is very low, the PBS volume can be appropriately reduced) and break the cells by repeated freezing and thawing or ultrasound. Centrifuge the extract at 2-8℃, 1500×g for 10 minutes, and take the supernatant for detection.\u003cbr\u003e5. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing.\u003cbr\u003e6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and assay.\u003cbr\u003e\u003cbr\u003ePre-Assay Preparation:\u003cbr\u003e1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature.\u003cbr\u003e2. Preparation of Standard Gradient Working Solution: Add 1mL of Universal Diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration is 5000pg\/mL). Then dilute to the following concentrations: 5000pg\/mL, 2500pg\/mL, 1250pg\/mL, 625pg\/mL, 312.5pg\/mL, 156.25pg\/mL, 78.125pg\/mL, and 0pg\/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 5000pg\/mL standard working solution into the first EP tube and mix thoroughly to make a 2500pg\/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250221\/c5275922094b48e3bc3f35f743d68dd5.png\" alt=\"\" width=\"468\" height=\"180\"\u003e\u003c\/div\u003e\n\u003cbr\u003e3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day.\u003cbr\u003e4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day.\u003cbr\u003e5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing).\u003cbr\u003e\u003cbr\u003eProcedure:\u003cbr\u003e1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C.\u003cbr\u003e2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.)\u003cbr\u003e3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used).\u003cbr\u003e4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes.\u003cbr\u003e5. Wash: Discard the liquid and wash the plate five times as in step 3.\u003cbr\u003e6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes.\u003cbr\u003e7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm.\u003cbr\u003e\u003cbr\u003eCalculation of experimental results:\u003cbr\u003eResult evaluation:\u003cbr\u003e1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis.\u003cbr\u003e2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal tobramycin antigen. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the universal tobramycin content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTobramycin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompetition Law\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 60%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96-Well Format Configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e remark \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Pre-coated 96-Well Plate \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e8-Well × 12 Strips\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e without \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2  Vials \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Universal diluent \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate  Biotin- Antibody ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e60uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.98%; text-align: center;\"\u003e\u003cdiv\u003e Concentrated enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center;\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 29.98%; text-align: center;\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e20× Washing Buffer \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e2×10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e Dilute as per instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e TMB Substrate Solution\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Stop Solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Sealing film \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e4 Sheets \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e1 \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 29.98%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e without \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTobramycin is an aminoglycoside antibiotic derived from Streptomyces tenebrioides used to treat various bacterial infections, particularly Gram-negative infections. It is particularly effective against Pseudomonas. It was patented in 1965 and approved for medical use in 1974. It is on the World Health Organization's List of Essential Medicines.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. \u003cbr\u003e2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. \u003cbr\u003e3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. \u003cbr\u003e4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. \u003cbr\u003e5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. \u003cbr\u003e6. Avoid direct exposure to strong light during storage and incubation. \u003cbr\u003e7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. \u003cbr\u003e8. Do not use expired products, and do not mix components with different product numbers and batches. \u003cbr\u003e9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. \u003cbr\u003e10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIf the unopened kit is stored at 4°C, the shelf life is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e78.12-5000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678620622923,"sku":"abs554146-96T","price":559.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/other-elisa-kit.oembed?page=23","provider":"AntBio","version":"1.0","type":"link"}