{"title":"Organoid Kit","description":"","products":[{"product_id":"human-liver-organoid-culture-medium-kit","title":"Human Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eby using ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue is digested with human normal liver primary tissue digestive solution C, and then shaken for 10-20min at 37 ℃ (observe the digestion at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human normal liver organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids are collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human normal liver organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul human normal liver organoid culture medium a. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman normal liver organoid culture medium is a medium that promotes the formation and expansion of human normal liver organoids in vitro. The product is a sterile liquid mixing system, containing amino acids, vitamins, organic and inorganic compounds and growth factors necessary to maintain the growth of target cells. The unique formula of this medium can provide a suitable nutritional environment for cells and promote the growth and expansion of human normal liver organoids in vitro\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 72.89%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eHuman normal liver organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eHuman normal liver primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303119947,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0a5342317f7d42e3a1b4f14be807cd10.jpg?v=1755257536"},{"product_id":"human-lung-organoid-culture-medium-kit","title":"Human Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal lung organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal lung primary tissue digestive fluid C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303152715,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d55581a68a16495ca0f93d6336ace34a.jpg?v=1755257536"},{"product_id":"human-pancreatic-organoid-culture-medium-kit","title":"Human Pancreatic Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition:\u003c\/strong\u003e \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal pancreatic organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal pancreas primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303218251,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/21b2e691926d4bb385e29ef9c99d000f.jpg?v=1755257536"},{"product_id":"mouse-intestinal-cancer-organoid-culture-medium-kit","title":"Mouse Intestinal Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse intestinal cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse intestinal cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303251019,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/639bda67e08d45d1a3eea7523863f8f3.jpg?v=1755257536"},{"product_id":"mouse-gastric-cancer-organoid-culture-medium-kit","title":"Mouse Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse gastric cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse gastric cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303316555,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4d8bcea19064307b46ca313008a2dac.jpg?v=1755257536"},{"product_id":"mouse-lung-cancer-organoid-culture-medium-kit","title":"Mouse Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse lung cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse lung cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303349323,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/df25ccbbcb584e9380fb9d45513b5c82.jpg?v=1755257536"},{"product_id":"mouse-breast-cancer-organoid-culture-medium-kit","title":"Mouse Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse breast cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse breast cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303414859,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2468206d87ef47faa202dc99ae5a81db_11ef8284-2a51-4fe7-9a24-72d605d8dc42.jpg?v=1755257536"},{"product_id":"mouse-hepatocarcinoma-organoid-culture-medium-kit","title":"Mouse Hepatocarcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition:\u003c\/strong\u003e \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse liver cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse liver cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303447627,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a01fad72163d4eaca96d77955ce10bef_076b9663-63cb-4083-8012-dd7b1200f550.jpg?v=1755257536"},{"product_id":"mouse-pancreatic-cancer-organoid-culture-medium-kit","title":"Mouse Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse pancreatic cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse pancreatic cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303513163,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/572bd8f3fe0c40b1b97b15a6fec5dc71_e3e23557-1501-47aa-8158-2276ffee6fb0.jpg?v=1755257536"},{"product_id":"bovine-mammary-glands-organoid-culture-medium-kit","title":"Bovine Mammary Glands Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eBovine mammary organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eCow breast primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303873611,"sku":null,"price":2000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8955c054bc07457bb95594276ad27b5d_f09b658b-e9d5-47a1-a990-96e1084b6b63.jpg?v=1755257536"},{"product_id":"mouse-gastric-organoid-culture-medium-kit","title":"Mouse Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse normal gastric organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse Gastric primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303906379,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7fdb1c69ce1344bfb31d00474cfa1df6_66fa0f25-560e-4c14-a782-34063ce7ed78.jpg?v=1755257536"},{"product_id":"human-gliomatosis-cerebri-organoid-culture-medium-kit","title":"Human Gliomatosis Cerebri Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A .\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman glioma organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman glioma primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303971915,"sku":null,"price":1250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/986a5a255b4d4e16b13e5c1721d14559_ed6e0a05-5794-4848-ad68-a90a5da99730.jpg?v=1755257536"},{"product_id":"human-prostate-cancer-organoid-culture-medium-kit","title":"Human Prostate Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u003cbr\u003e(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u003cbr\u003e(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u003cbr\u003e(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u003cbr\u003e(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u003cbr\u003e(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman prostate cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman prostate cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304004683,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/210383513c4b45aea8f09250567833f1_3c8f120c-301c-4543-aaa1-7fb2bdd97d4b.jpg?v=1755257536"},{"product_id":"mouse-ovary-organoid-culture-kit","title":"Mouse ovary Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal ovarian tissue of mice after sampling must be cleaned at 2 - 8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the culture dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3 mm\u003csup\u003e3\u003c\/sup\u003eusing ophthalmic scissors or scalpel\u003cbr\u003e(3) the tissue was digested with the digestive juice C of mouse normal ovary primary tissue, and then was shaken at 37 ℃ for 15-20 min. (the digestion condition was observed at any time during the digestion process)\u003cbr\u003e(4) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 6, observe the collected tissue volume, add 25-30 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add mouse normal ovarian organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul mouse normal ovarian organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal ovarian organoid culture medium a.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse normal ovarian organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse ovary primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304070219,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/67441c21a7c84d678af73114304aa8dc_27e51f89-0ca0-45f2-ab9c-c8f2f550691a.jpg?v=1755257536"},{"product_id":"lung-cancer-like-organ-culture-medium-kit","title":"Human Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eby using ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue was digested with human lung cancer primary tissue digestive solution C, and the shaking digestion was carried out at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human lung cancer organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids are collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human lung cancer organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human lung cancer organoid culture medium a. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 67.0951%; height: 157px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 10px;\"\u003eHuman lung cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 10px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px;\"\u003eHuman lung cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px;\"\u003eOrganoid primary digest D\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8963%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months-20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199930955,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b05a5dd955ef4734accf2479ad310fed.jpg?v=1755257710"},{"product_id":"colorectal-cancer-organ-culture-medium-kit","title":"Human Colorectal cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003ctaking a well cell culture plate as an example apply of tissue matrix adhesive mixture to each for laying at place the laid in incubator minutes form gel and add porcine endometrial organoid medium room temperature cultivation\u003e\u003c\/taking\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cafter collecting types of organs add matrix glue and resuspend. place ul per well on a cell culture plate it in box for minutes. porcine endometrial organoid medium\u003e\u003c\/after\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath\u003cduring the water bath melting process it is necessary to gently shake freezing tube ensure that frozen solution completely melts within minutes quickly transfer dissolved organoid cells a centrifuge blow times with pipette for then remove supernatant and collect cell precipitates. add an appropriate amount of passage culture buffer g centrifugation\u003e\u003c\/during\u003e(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 66.4962%; height: 154px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman colorectal cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman colorectal cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 18px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 10px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199963723,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/934676f47c484cf3b545dcb5e918f890.jpg?v=1755257710"},{"product_id":"breast-cancer-like-organ-culture-medium-kit","title":"Human Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u003cbr\u003e(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u003cbr\u003e(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u003cbr\u003e(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u003cbr\u003e(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u003cbr\u003e(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u003cbr\u003e(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman breast cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman breast cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months-20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199996491,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/41b26b5d6af4455e9f511cadc064d53f.jpg?v=1755257710"},{"product_id":"pancreatic-cancer-organ-like-culture-medium-kit","title":"Human Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman pancreatic cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman pancreatic cancer primary tissue digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200029259,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c3ec8d6768984dec9f80aca4da2747d7.jpg?v=1755257710"},{"product_id":"endometrial-cancer-like-organ-culture-medium-kit","title":"Human Endometrial carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eusing ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue was digested with human endometrial cancer primary tissue digestive solution C, and then shaken for 10-20min at 37 ℃ (observe the digestion at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human endometrial cancer organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids are collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human endometrial cancer organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of human endometrial cancer organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman endometrial cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman endometrial cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200062027,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ad775ba20e984e20802f7f09f374c718.jpg?v=1755257710"},{"product_id":"gastric-cancer-like-organ-culture-medium-kit","title":"Human Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eusing ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue was digested with human gastric cancer primary tissue digestive solution C, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human gastric cancer organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human gastric cancer organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human gastric cancer organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman gastric cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman gastric cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200094795,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8809bd92b4ab4dc69202aa4cf93775d3.jpg?v=1755257709"},{"product_id":"mouse-intestinal-organ-culture-medium-reagent","title":"Mouse Intestinal Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) put the normal intestinal tissue of mice after sampling into the sampling bottle of pre cooled (2-8° c) tissue preservation solution e, and quickly transfer it to the clean laboratory for tissue processing and stem cell separation, take photos and register information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of small intestine tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(4) the tissue was digested with the primary tissue digestive solution c of mouse normal intestine, and then it was shaken at 4 ℃ for 10min (the digestion condition was observed at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add mouse normal intestinal organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organ collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of mouse normal intestinal organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal intestinal organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 65.7289%; height: 164px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003eMouse normal intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003ePrimitive organization mice intestine digestive juices C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9808%; height: 10px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 33.3668%; height: 10px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months-20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518201700427,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ecc367dccfd84bcdbd5ce9c78527050a.jpg?v=1755257710"},{"product_id":"mouse-lung-organ-culture-medium-kit","title":"Mouse Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition:\u003c\/strong\u003e \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse normal lung organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse lung primary tissue digest C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months-20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202257483,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2791feb33ce54effb9c66902b2806fe3.jpg?v=1755257710"},{"product_id":"mouse-liver-like-organ-culture-medium-kit","title":"Mouse Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eby using ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue was digested with the digestive solution c of primary mouse normal liver tissue, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add mouse normal liver organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of mouse normal liver organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal liver organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eComposition:\u003c\/strong\u003e \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 72.89%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eMouse normal liver organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eMouse liver primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50.2098%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months;  -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202290251,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2dd5697825f64148b0ab220b6bbca114.jpg?v=1755257710"},{"product_id":"ovarian-cancer-organoid-culture-medium-kit","title":"Human Ovarian Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman ovarian cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman ovarian cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202486859,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/929bd7968a8947328acd5d58c49fbeab_aba21433-0d5f-4c1c-86bc-67a1a926254a.jpg?v=1755257710"},{"product_id":"pig-endometrial-like-organ-culture-medium-kit","title":"Pig Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDomposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePorcine endometrial organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePorcine endometrial primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202552395,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/121c974eac4845bd9be8f5b79e66fea8_90ee7a31-92e3-43a8-b28c-056a54fed026.jpg?v=1755257710"},{"product_id":"mouse-endometrial-like-organ-culture-medium-kit","title":"Mouse Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information\u003cbr\u003e(2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby\u003cbr\u003e(3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eusing ophthalmic scissors or scalpel\u003cbr\u003e(4) the tissue was digested with mouse endometrial primary tissue digestive solution C, and then shaken for 10-20min at 37 ℃ (observe the digestion at any time during the digestion process)\u003cbr\u003e(5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add mouse endometrial organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp;Nbsp\u0026amp;Nbsp\u0026amp;Nbsp  b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul mouse endometrial organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse endometrial organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eMouse endometrial primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202617931,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/57abf61d25b44bffbb678758e0b9ccc3_12f27ac7-88bd-4e05-a95d-911df3067779.jpg?v=1755257710"},{"product_id":"gastric-organoid-culture-medium-kit","title":"Human Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal gastric organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman normal gastric primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202650699,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/666c3b220bed4e93b8fd03d315eb4c69_3e2cf3a4-3c68-497f-afcf-8f66fa48819c.jpg?v=1755257710"},{"product_id":"nasopharyngeal-carcinoma-organoid-culture-medium-kit","title":"Human Nasopharyngeal Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman nasopharyngeal carcinoma organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman nasopharyngeal carcinoma primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months; -20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202683467,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0963b81d0ebe4843be4873f36ddb1525_2f620f80-0efe-46fc-bfcf-1955a13376c3.jpg?v=1755257710"},{"product_id":"organotial-cervical-cancer-organoid-culture-medium-kit","title":"Human Cervical Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u0026lt;(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u0026lt;(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u0026lt;(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u0026lt;(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u0026lt;(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003e\u0026amp;nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp\u0026amp; Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u0026lt;(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman cervical cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eHuman cervical cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4° C. 3months-20° C. 1 year, see label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202978379,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/24e720601b154c7aae5f1e558181ce3c_dd58d3ad-8ef9-4c04-9aed-824037935fea.jpg?v=1755257710"},{"product_id":"mouse-small-intestine-organoid-culture-kit","title":"Organotypic balb\/c mouse normal small intestinal organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u003cbr\u003e(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u003cbr\u003e(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u003cbr\u003e(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)\u003cbr\u003e(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u003cbr\u003e(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(Balb\/C) Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eBalb\/c mouse normal small intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eBalb\/c mice normal small intestine primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204125259,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d9618b188d7c44bdbf6c97ffe978544e_afd4efb8-c05b-418e-89db-d340ec2fe11d.jpg?v=1755257710"},{"product_id":"mouse-colon-organoid-culture-kit","title":"Organotypic balb\/c mouse normal colon organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal colonic tissue of balb\/c mice after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of Balb\/c mouse colon tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(3) the tissues were digested with the digestive juice C of the primary normal colon tissues of balb\/c mice, with shaking digestion at 4 ℃ for 20-25min or standing digestion for 40-50min (observe the digestion at any time during the digestion process)\u003cbr\u003e(4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add balb\/c mouse normal colon organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of balb\/c mouse normal colon organoid culture medium A\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of balb\/c mouse normal colon organoid medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(Balb\/C) Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eBalb\/c mouse normal colon organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eBalb\/c mice normal colon primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204158027,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/20b30b219dd94b2cb6ddbfdc0de356be_2c96e0de-6bca-49f3-aa48-d92383a21abe.jpg?v=1755257710"},{"product_id":"pig-lung-organoid-culture-kit","title":"Organotial porcine normal lung organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information\u003cbr\u003e(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use\u003cbr\u003e(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm\u003cbr\u003e(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)\u003cbr\u003e(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion\u003cbr\u003e(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge\u003cbr\u003e(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board\u003cbr\u003e\u003cstrong\u003e2. Organ like passage culture\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4\u003cbr\u003e\u003cstrong\u003e3. Organ like cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes\u003cbr\u003e(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organ like resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube\u0026lt;(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePig lung Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePorcine normal lung organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePig lung primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204190795,"sku":null,"price":1167.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e1a2d91847724e63b5e92b3aac5602a3_0c38d72c-ea9d-4a1a-9e7b-0a02904b3c6b.jpg?v=1755257710"},{"product_id":"fetal-rat-small-intestine-organoid-culture-kit","title":"Organotal rat fetal normal small intestinal organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal small intestine tissues of fetal rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of fetal rat small intestine tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(3) the tissue was digested with the digestive solution c of the primary tissue of the normal small intestine of rat fetus, with shaking digestion at 4 ℃ for 20-25min or standing digestion for 40-50min (observe the digestion at any time during the digestion process)\u003cbr\u003e(4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add rat fetal rat normal small intestinal organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat fetal normal small intestinal organoid culture medium A\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat fetal normal small intestinal organoid medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat fetal rat normal small intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eFetal Rat Small Intestine primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204223563,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/530c406a65a3429d94ceaad84485ff54_39a610b2-c752-4d09-a6d5-9b1fc9efd240.jpg?v=1755257710"},{"product_id":"fetal-rat-colon-organoid-culture-kit","title":"Organotal rat fetal normal colon organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal colonic tissue of fetal rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of fetal rat colon tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(3) the tissue was digested with the digestive solution c of the primary tissue of the normal colon of rat fetus, and then it was shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion)\u003cbr\u003e(4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add rat fetal normal colon organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat fetal normal colon organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat fetal normal colon organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat fetal normal colon organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eFetal Rat Colon primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204256331,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/01dcfb14b0fd4b718a315d98713edc06_999e6498-4db1-4976-88b6-7acb619cc85a.jpg?v=1755257710"},{"product_id":"rat-small-intestine-organoid-culture-kit","title":"Organotal rat normal small intestinal organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal small intestine tissues of rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of normal small intestine tissue of rats with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(3) the tissue was digested with rat normal small intestine primary tissue digestive solution C, and then shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion process)\u003cbr\u003e(4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add rat normal small intestinal organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat normal small intestinal organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of rat normal small intestinal organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat normal small intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat Small Intestine primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206386251,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c079c22e2a6c4af3875af125b875a7bf_6cc75bf8-ab89-460e-a26f-eae74b8f39f6.jpg?v=1755257710"},{"product_id":"rat-colon-organoid-culture-kit","title":"Organotal rat normal colon organoid culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary \u003c\/strong\u003e\u003cbr\u003e(1) the normal colonic tissue of rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added)\u003cbr\u003e(2) put the tissue into the Petri dish, wash it with primary culture buffer B for three times, remove impurities, remove the intestinal mucosa of normal colon tissue of rats with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm\u003csup\u003e3\u003c\/sup\u003e\u003cbr\u003e(3) the tissue was digested with rat normal colon primary tissue digestive solution C, and then shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion process)\u003cbr\u003e(4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion\u003cbr\u003e(5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation\u003cbr\u003e(6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate\u003cbr\u003e(7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃)\u003cbr\u003e(8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add rat normal colon organoid medium a (restore room temperature) for culture\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4\u003cbr\u003e(4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat normal colon organoid culture medium a\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e(1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min\u003cbr\u003e(2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) \u003cbr\u003e(3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid\u003cbr\u003e(4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube\u003cbr\u003e(5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube\u003cbr\u003e(2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting\u003cbr\u003e(3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min\u003cbr\u003e(4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g\u003cbr\u003e(5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat normal colon organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eComposition: \u003cbr\u003e\u003ctable style=\"border collapse: collapse; width: 71.1055%; height: 169px; margin: 0 Auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%; text align: Center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text align: Center;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat normal colon organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eRat colon primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.4551%;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 ℃, 3months; -20 ℃, 1 year, see the label for details\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206419019,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/26266b426fd144098f5a6ae64f2d1455_6b7a568f-47ca-4b5c-a4dd-a988c129c9c0.jpg?v=1755257711"},{"product_id":"esophagus-cancer-organoid-culture-kit","title":"Organotial Human Esophageal Cancer Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEsophagus Cancer Organoid  Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"width: 56.8817%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; text-align: center; height: 22px;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; text-align: center; height: 22px;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eHuman Esophagus Cancer Organoid A\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eHuman Esophagus Cancer Primary tissue digests C\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eOrganoid passage digests D\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eOrgans of freeze-stored solution F\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 45.5367%; height: 22px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 12.7291%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) tissue after harvesting should be stored in pre-cooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. \u003cbr\u003e(2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissues were digested with human esophageal cancer primary tissue digestion solution C and shaken at 37℃ for 10-20min (the digestion was observed at any time during the digestion). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. \u003cbr\u003e(7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. \u003cbr\u003e(8) For the example of 24-well cell culture plates, 25ul of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). \u003cbr\u003e(9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to form gelatin, and human esophageal cancer organoid medium A was added for culture (return to room temperature). \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was sucked out by pipetting gun, and 1-2 mL of 4℃ organoid subculture buffer G was added to each well for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15ml centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5ml centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5ml centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. \u003cbr\u003e(4) After the organoid was collected, 25ul of Matrigel was added to the 24-well cell culture plate, and 500ul of human esophageal cancer organoid medium A was added to the incubator for 10-15min. \u003cbr\u003e\u003cstrong\u003e3. Organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The culture medium was removed by pipetting gun, and 1-2ml of 4℃ organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15ml centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4ml. \u003cbr\u003e(5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10 mL organoid subculture buffer G was placed in a 15 mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15ml centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5ml centrifuge tube and centrifuged at 300g for 5min. \u003cbr\u003e(5) Matrigel was resuspended, 25ul of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500ul of human esophageal cancer organoid medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206484555,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3efac298f03d4c5795596bd5eb4f8924_40d7aac4-ddcc-44e5-85d6-5b8c72360df2.jpg?v=1755257710"},{"product_id":"bladder-cancer-organoid-culture-kit","title":"Organotial Human bladder Cancer organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003ekit composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: justify;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: justify;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eHuman bladder cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eHuman bladder cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) After sampling the tissue should be placed in pre-cooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. \u003cbr\u003e(2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissue was digested with human bladder cancer primary tissue digestion solution C, and the digestion was shaken at 37℃ for 10-20min (the digestion was observed at any time during digestion). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. \u003cbr\u003e(7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. \u003cbr\u003e(8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). \u003cbr\u003e(9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to form glue, and human bladder cancer organoid medium A (restored to room temperature) was added for culture. \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was sucked out by pipetting gun, and 1-2mL of 4℃ organoid subculture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. \u003cbr\u003e(4) After the organoids were collected, stromal glue was added and resuspended. Each well of stromal glue was spread in A 24-well cell culture plate, and placed in an incubator for 10-15min to add 500uL human bladder cancer organoid medium A. \u003cbr\u003e\u003cstrong\u003e3. Organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The medium was removed by suction with a pipettes gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. \u003cbr\u003e(5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. \u003cbr\u003e(5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of human bladder cancer organoid culture medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206517323,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f16f15012e4c427c8cce17338682039d_ca618ff0-210c-4d5f-8e38-d3fe559ec547.jpg?v=1755257710"},{"product_id":"pig-hepar-organoid-culture-kit","title":"Normal liver organ class training kit Organotial pig","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) The harvested tissues should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. \u003cbr\u003e(2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissues were digested with porcine normal liver primary tissue digestion solution C and shaken at 37℃ for 15-30min (the digestion was observed at any time during the digestion). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. \u003cbr\u003e(7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. \u003cbr\u003e(8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). \u003cbr\u003e(9) The laid culture plate was placed in an incubator at 37 ° C for 10-15min to become gelation, and porcine normal liver organoid medium A was added for culture (return to room temperature). \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was sucked out by pipetting gun, and 1-2mL of 4℃ organoid subculture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. \u003cbr\u003e(4) After the organoids were collected, 25uL of matrigel was added to the 24-well cell culture plate, and 500uL of pig normal liver organoid medium A was added to the incubator for 10-15min. \u003cbr\u003e\u003cstrong\u003e3, organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. \u003cbr\u003e(5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. \u003cbr\u003e(5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of porcine normal liver organoid medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206550091,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/83f8850d3fb34d39bc43cfa57e9b6c77_d2f9e4e8-985a-42d9-b4ba-d01309846550.jpg?v=1755257710"},{"product_id":"icc-organoid-culture-kit","title":"Organotial Human hepatobiliary carcinoma organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: justify;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman hepatic bile duct carcinoma organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman hepatic bile duct carcinoma  primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) The harvested tissue should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. \u003cbr\u003e(2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissues were digested with human hepatobiliary carcinoma primary tissue digestion solution C and shaken at 37℃ for 15-25min (the digestion was observed at any time during the digestion). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. \u003cbr\u003e(7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. \u003cbr\u003e(8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). \u003cbr\u003e(9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to form gelatin, and then cultured with human hepatobiliary carcinoma organoid medium A (restored to room temperature). \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was sucked out by pipetted gun, and 1-2 ml of 4℃ organoid subculture buffer G was added to each well for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. \u003cbr\u003e(4) After the organoids were collected, 25uL of matrigel was added to the 24-well cell culture plate, and 500uL of human hepatobiliar carcinoma organoid medium A was added to the incubator for 10-15min. \u003cbr\u003e\u003cstrong\u003e3. Organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The medium was removed by suction with a pipettes gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. \u003cbr\u003e(5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. \u003cbr\u003e(5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of human hepatobiotic carcinoma organoid medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206779467,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/742d5b1e3b1f4aefa56af6ea64ffbcc6_7ec72757-db57-42d3-a845-a8d571afcac7.jpg?v=1755257710"},{"product_id":"esophagus-organoid-culture-kit","title":"Organotial Human Normal esophageal organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003eComposition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: justify;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman esophagus cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman esophagus cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) The harvested tissues should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. \u003cbr\u003e(2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissues were digested with normal human esophageal primary tissue digestion solution C and shaken at 37℃ for 20-25min (the digestion was observed at any time during the digestion). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. \u003cbr\u003e(7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. \u003cbr\u003e(8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). \u003cbr\u003e(9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to become gelation, and human normal esophageal organoid medium A was added for culture (return to room temperature). \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. \u003cbr\u003e(4) After the organoid was collected, 25uL of Matrigel was added to the 24-well cell culture plate, and 500uL of human normal esophageal organoid medium A was added to the incubator for 10-15min. \u003cbr\u003e\u003cstrong\u003e3. Organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4℃ organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. \u003cbr\u003e(5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. \u003cbr\u003e(5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of human normal esophageal organoid medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206812235,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/eebd48a3344a4b8fb2a880f947fcc360_2d6b55ef-8258-4d7f-9efa-e6da044252cc.jpg?v=1755257711"},{"product_id":"renal-cell-carcinoma-organoid-culture-kit","title":"Organotial kidney organ class training kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003eComposition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text align: Center;\"\u003eComponent name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: justify;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman kidney cancer organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eHuman kidney cancer primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px;\"\u003eOrganoid cryopreservation fluid F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px;\"\u003eOrganoid subculture buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, primary \u003c\/strong\u003e\u003cbr\u003e(1) tissue after sampling suggested 2-8° The tissues were stored under C conditions and quickly transferred to a clean laboratory for tissue processing and cell separation. The information was photographed and registered. \u003cbr\u003e(2) Several culture dishes were prepared and primary culture buffer B, precooled at 4 ° C, was added for later use. \u003cbr\u003e(3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm\u003csup\u003e3\u003c\/sup\u003ein volume using ophthalmic scissors or scalpel. \u003cbr\u003e(4) The tissues were digested with human renal cancer primary tissue digestion solution C and shaken at 37℃ for 15-25min (the digestion was observed at any time during the digestion process). \u003cbr\u003e(5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. \u003cbr\u003e(6) The filtrate was filtered by a sieve with a pore size of 100um. After collecting the filtrate, the supernatant was removed after enrichment and centrifugation at 300g for 5min. The supernatant was resuspended by adding primary culture buffer B, and the supernatant was removed after enrichment and centrifugation at 300g for 5min. \u003cbr\u003e(7) The cell precipitate contained red blood cells, the supernatant was discarded, 1-2 ml of red blood cell lysate was added for 1-2min, diluted to 10mL, and centrifuged at 300g for 5min. \u003cbr\u003e(8) Matrix glue calculation: The collected tissue volume was observed after step 6, and the paving plate was resuspended by adding matrix glue (abs9495) at 25 times the tissue volume. \u003cbr\u003e(9) For the example of 24-well cell culture plate, 25uL-30uL tissue matrix glue mixture was dispensed to each well for paving (\u003cstrong\u003eNote: \u003c\/strong\u003ematrix glue was maintained at 0-4 ° C for operation). \u003cbr\u003e(10) The paved culture plate was placed in an incubator at 37 ° C for 10-15min. After the matrix gel solidified, 500-750uL of human renal cancer organoid medium A (returned to room temperature) was added for culture. \u003cbr\u003e\u003cstrong\u003e2, Organoid subculture \u003c\/strong\u003e\u003cbr\u003e(1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube, and allowed to rest for 10-15min at 4 ° C. (every 6-8 Wells are a group) \u003cbr\u003e(3) a: When the number of organoids is insufficient or the volume is small: Discard the supernatant by centrifugation at 300g for 5min, add 1mL organoid passage culture buffer G, resuspension and transfer into 1.5mL centrifuge tube, and discard the liquid by centrifugation at 300g for 5min for the fourth step. \u003cbr\u003e          b: When organoids are large in number or size: Centrifugation at 300g for 5min to discard the supernatant, add 1-2mL organoid passage digestion solution D for 2-4min, add 3 times the volume of organoid passage culture buffer G to terminate digestion, centrifugation at 300g for 5min to discard the supernatant, add an appropriate amount of organoid passage culture buffer G to resuspend and transfer to a 1.5mL centrifuge tube. The supernatant was discarded by centrifugation at 300g for 5min, and the fourth step was carried out. \u003cbr\u003e(4) After organoids were collected, they were resuspended by adding matrigel, and 25uL of Matrigel per well was spread in 24-well cell culture plates and placed in an incubator for 10-15min. After the Matrigel had set, 500 to 750uL of human renal cancer organoid medium A was added to each well. \u003cbr\u003e\u003cstrong\u003e3, organoids were frozen \u003c\/strong\u003e\u003cbr\u003e(1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. \u003cbr\u003e(2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) \u003cbr\u003e(3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. \u003cbr\u003e(4) Add 2mL organoid cryopreservation solution F to every 3 Wells, gently blow and mix, and transfer to cell cryopreservation tubes, 1mL per tube. \u003cbr\u003e(5) Make the labeling information, after the program cooling, move to -80° C refrigerator, 48h later, transferred to liquid nitrogen for long-term storage. Or put it into the 4℃ refrigerator for 40min, put it into the -20℃ refrigerator for 2h, move it to the -80℃ refrigerator, and put it into the liquid nitrogen tank for storage after 48h. \u003cbr\u003e\u003cstrong\u003e4, organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e(1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. \u003cbr\u003e(2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. \u003cbr\u003e(3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted in a short time. \u003cbr\u003e(4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended, then transferred to a 1.5mL centrifuge tube and centrifuged at 300g for 5min, and the supernatant was discarded. \u003cbr\u003e(5) Add 100ul of Matrigel to each tube of cryopreserved tube and resuspend, 25uL of Matrigel in each well was spread in 24-well cell culture plate, placed in the incubator for 10-15min to gel, and 500-750uL of human renal cancer organoid medium A was added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518207762507,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0ce8f4d12caa483391bf6f4c6db7c2af_9c65a312-3ebe-42c3-98f7-33bd9ea70fe5.jpg?v=1755257710"},{"product_id":"carcinoma-of-the-larynx-organoid-culture-kit","title":"Organotial Human laryngeal carcinoma organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCarcinoma of the larynx  Organoid  Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 100%; height: auto;\" border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 15.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 15.00pt; width: 49.50pt;\" width=\"99\" height=\"30\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 49.50pt;\" width=\"99\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 45.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 45.00pt; width: 49.50pt;\" width=\"99\" height=\"90\"\u003eHuman laryngeal cancer organoid culture Medium A\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 30.00pt; width: 49.50pt;\" width=\"99\" height=\"60\"\u003eOriginal generation of cultivating buffer B\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 45.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 45.00pt; width: 49.50pt;\" width=\"99\" height=\"90\"\u003ePrimitive organization digestive juices C throat cancer\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 45.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 45.00pt; width: 49.50pt;\" width=\"99\" height=\"90\"\u003eOrganoid passage digest D\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 30.00pt; width: 49.50pt;\" width=\"99\" height=\"60\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 30.00pt; width: 49.50pt;\" width=\"99\" height=\"60\"\u003eOrganoid cryopreservation solution F\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 45.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 45.00pt; width: 49.50pt;\" width=\"99\" height=\"90\"\u003eOrgan class subculture buffer G\u003c\/td\u003e\n\u003ctd class=\"et3\" style=\"width: 49.50pt;\" width=\"99\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eIsotype\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Human laryngeal cancer organoid culture medium A can be stored at 4 ℃ for 3 months. It is recommended to use it within 1 month after receiving goods at 4 ℃, and it is not recommended to store it at -20 ℃ for a long time to avoid repeated freezing and thawing more than 2 times. \u003cbr\u003e2.  Tissue preservation solution E and human laryngeal cancer primary tissue digestion solution C contain nutrients to maintain cell activity. In order to maintain the activity of nutrients, it is not recommended to store at -20 ℃ for a long time to avoid repeated freezing and thawing more than 2 times. \u003cbr\u003e3. In 2– The matrix glue was thawed overnight at 8 ° C ambient conditions. When using matrix glue, place it in an ice box to prevent premature solidification. The matrix glue forms a gel within 20 min at 37 ° C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eTaking 24-well plate as an example \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eI. Sample pretreatment \u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: primary culture buffer B (precooled at 4 ℃), tissue preservation solution E, sampling tube, tissue transport box, ice pack \u003cbr\u003e2. Sample sampling and transportation: the tissue should be placed in tissue preservation solution E within 30 min in vitro, the primary culture buffer B should be washed 3-5 times, the blood on the surface of the tissue should be washed, put into tissue preservation solution E, and the sampling tube should be stored and transported at 4 ℃ for 72 hours. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eII. Primary Culture of organoids \u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: \u003cbr\u003e(1) Materials to be prepared: Matrix glue (melt in 4 ℃ refrigerator 24 h in advance), tweezers (10 cm), pointed ophthalmic surgical scissors\/surgical blade, disposable 60 mm Petri dish, 1.5 mL\/15 mL\/50 mL EP tube, 100 um cell filter, 3 mL Papanicolaou straw \/1000 uL pipettes gun, 24-well cell culture plate, metal ice box. \u003cbr\u003e(2) Kit reagents: Primary culture buffer B (4 ° C), primary tissue digestion buffer C (37 ° C), human laryngeal cancer organoid medium A (room temperature or 37 ° C). \u003cbr\u003e2. Organoid construction: \u003cbr\u003e(1) Laryngeal cancer tissues after sampling are recommended to be stored and transported at 2-8 ℃, and quickly transported to a clean laboratory for the experimental process of human laryngeal cancer organoid construction. The tissues are taken photos and the detailed information is registered. \u003cbr\u003e(2) After the sampling tube was disinfected, the tissue was removed on the ultra-clean table and placed in the culture dish. The primary culture buffer B was added, and the cleaning operation was repeated for 3 or more times with a 3 mL papanicolaher pipette or 1000 uL pipetting knife. \u003cbr\u003e(3) Remove the tissue impurities with ophthalmic scissors or surgical blade, transfer the forceps to 1.5 mL EP tube, further mechanically dissociate the tissue into a volume of 1-3 mm\u003csup\u003e3\u003c\/sup\u003etissue block with ophthalmic scissors, and transfer to 15 mL EP tube. Then 5 mL of human laryngeal cancer tissue digestion solution was added and digested at 37 ℃ for 15-25 min. During the digestion process, the tissue digestion was observed under the microscope every 10 min. A small amount of digestive fluid was taken for observation under the microscope, and more cell clusters or single cells below 70 um were observed before the next operation. \u003cstrong\u003eNote: \u003c\/strong\u003eIf the amount of tissue was too small or the biopsy tissue was digested with 1 mL of human laryngeal cancer primary tissue digestion solution C in 1.5 mL EP tube. \u003cbr\u003e(4) After digestion, the tissues were filtered through a 100 um pore size cell screen, and the filtrate was collected. The digestion was terminated by rinsing with 3 times the volume of primary culture buffer B. The supernatant was discarded after enrichment and centrifugation at 300 g for 5 min. If the cell precipitate contained red blood cells, 1-2 mL red blood cell lysate was added for 1-2 min, diluted to 10 mL, and the supernatant was discarded after enrichment and centrifugation at 300 g for 5 min. \u003cstrong\u003eNote: \u003c\/strong\u003ewhen there is too little precipitation or no red blood cells, the tumor organoid culture is performed directly. \u003cbr\u003e(5) The volume of cell precipitates collected by centrifugation was observed and resuspended by adding 25 times the volume of matrix glue to form a 3D culture space structure, and bubbles were avoided during the resuspension process. The volume of cell precipitation is shown in the figure below, and 200 uL, 150 uL, 100 uL and 50 uL of matrix glue were added respectively according to the amount of cell precipitation in the figure. \u003cdiv style=\"text-align: justify;\"\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/318431bcb65e4dc9a8be5eed4bcf2962.png\" alt=\"\" width=\"250\" height=\"127\"\u003e\u003cbr\u003e\u003cp style=\"text-align: justify;\"\u003eexample: 24 hole cell culture plate according to the uL 25-30 uL\/hole point glue, \u003cstrong\u003ematrix  strong \u0026gt;\u003cstrong\u003eall  strong \u0026gt;\u003cstrong\u003eat 0 to 4  strong \u0026gt;\u003cstrong\u003e\u0026amp; have spent \u003c\/strong\u003e\u003cstrong\u003eoperation under the condition of \u003c\/strong\u003e℃. The cell culture plate was placed in A 37 ° C incubator for 10-15 min, and after the matrix gel had solidified, 500-750 uL of human laryngeal cancer organoid medium A (preheated at 37 ° C) was added to each well for culture. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIII. Organoid subculture \u003cbr\u003e\u003c\/strong\u003e1. Experimental materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be prepared: matrix glue (melt in 4 ℃ refrigerator 24 hours in advance), 1.5 mL\/15 mL EP tube, 3 mL Papanicolaek pipette \/1000 uL pipette gun, 24-well cell culture plate, metal ice box. \u003cbr\u003e(2) Kit reagent: organoid passage culture buffer G (4 ℃), organoid passage digestion solution D (37 ℃), human laryngeal cancer organoid culture medium A (room temperature or 37 ℃). \u003cbr\u003e2. Organoid passage: \u003cbr\u003e(1) Select appropriate organoids for passage, generally grow for about a week, more than 20 organoids can be seen under a microscope at 10X, or organoids with a size of 100-200 um. The same volume of organoid passage culture buffer G was added to each well. The matrix glue was gently blown off by a pipetting gun, collected in 15 mL EP tubes, transferred to an EP tube every 6-8 Wells, and left for 10-15 min at 4 ° C. \u003cbr\u003e(2) Determine whether digestion and passage are necessary according to the growth of the organoids. \u003cstrong\u003eNote: \u003c\/strong\u003eAfter centrifugation, if there is less precipitation at the bottom of the tube, no cells are seen, and the matrix glue is not stratified, it can be resuspended again, the centrifugal force can be increased, and then centrifuged again. \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003ea: When the number of organoids was insufficient or the volume was small, the supernatant was discarded by centrifugation at 300 g for 5 min. \u003cbr\u003eb:  When the number of organoids is large or large, 300  g centrifuge 5  The supernatant was discarded in min and either digested with digester or mechanically. \u003cbr\u003e    Digestion with digestive fluid: Add 1-2 mL organoid passage digestion solution D, blow off the cell precipitate, digest at room temperature for 2-4 min, blow every minute, observe under the microscope, until the digestion to the state (FIG. A-B) can be stopped. The digestion was terminated by adding 3 times the volume of organoid digestion liquid in organoid passage culture buffer G, and the supernatant was discarded by centrifugation at 300 g for 5 min. \u003cbr\u003e    Mechanical digestion: Add 1 mL organoid passage culture buffer G, blow with 1 mL pipettes for 20-30 times until blowing to (FIG. C-D) state, add 10 mL passage buffer, centrifugation at 300 g for 5 min and discard the supernatant. \u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/76fb16898e414dc1a8ef51ee427fc05b.png\" alt=\"\" width=\"387\" height=\"233\"\u003e\u003cbr\u003e(3) Observe the volume of organoid precipitation collected by centrifugation. If the precipitation is very small, 1 times the volume of precipitation supernatant can be reserved for gelatin injection, a lot of precipitation supernatant can be absorbed, add 25 times the volume of organoid precipitation matrix glue to resuspend the organoid. Example: The 24-well cell culture plate was dispensed at 25 ul-30 uL\/ well, and the matrix glue was maintained at 0-4 ℃ throughout the operation . The cell culture plate was placed in A 37 ℃ incubator for 10-15 min, and after the matrix gel solidified, 500-750 uL of human laryngeal cancer organoid medium A (preheated at 37 ℃) was added to each well for culture. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Organoid Cryopreservation \u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Self-supplied materials: 15 mL EP tube, cell cryopreservation tube, program cooling box, pipeting gun. \u003cbr\u003e(2) Kit reagent: organoid passage culture buffer G (4 ℃), organoid freezing solution F. \u003cbr\u003eOrganoid cryopreservation: \u003cbr\u003eorganoids not in use should be frozen and stored in a low temperature environment. \u003cbr\u003e(1) The medium was sucked out, and the equal volume of organoid passage culture buffer G was added to each well. The matrix glue was gently blown off by a pipetting gun, collected in 15 mLEP tubes, transferred to an EP tube every 6-8 Wells, and left for 10-15 min at 4 ℃. The supernatant was discarded by centrifugation at 300 g for 5 min. Then 2 mL organoid cryopreservation solution F was added to every three Wells, gently blown and mixed, and transferred to cell cryopreservation tubes (1 mL per tube). \u003cbr\u003e(2) The labeled information was put into the program cooling box, transferred to the refrigerator at -80 ℃, and stored in a liquid nitrogen tank after 48 hours. Or put it in the 4 ℃ refrigerator for 40 min, put it in the -20 ℃ refrigerator for 2 h, move it to the -80 ℃ refrigerator, and put it in the liquid nitrogen tank after 48 h. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eV. Organoid resuscitation and culture \u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be prepared: matrix glue (melt in 4 ℃ refrigerator 24 hours in advance), 15 mL EP tube, water bath, pipettes gun, 24-well cell culture plate, ice box. \u003cbr\u003e(2) Kit reagent: organoid passage culture buffer G (4 ℃), human laryngeal cancer organoid culture medium A. \u003cbr\u003e2. Resuscitation culture of organoids: \u003cbr\u003e(1) The frozen organoids were removed from the low temperature environment and quickly thawed in a 37 ℃ water bath. During the process of water bath melting, it was necessary to gently shake the frozen tube to ensure that the frozen solution was completely thawed in a short time. The thawed organoids were quickly transferred to a 15 mLEP tube, gently blown by a pipettes gun for 6-8 times, and centrifuged at 300 g for 5 min to discard the supernatant. An appropriate amount of passage buffer G was added and resuspended, transferred to a 1.5 mL EP tube and centrifuged at 300 g for 5 min, and the supernatant was discarded. \u003cbr\u003e(2) 100 uL of Matrigel was added to each cryopreservation tube and resuspended. The 24-well cell culture plate was dispensed at 25 ul-30 uL\/ well, and the matrigel was maintained at 0-4 ℃ throughout the operation. The cell culture plate was placed in A 37 ℃ incubator for 10-15 min, and after the matrix gel solidified, 500-750 uL of human laryngeal cancer organoid medium A (preheated at 37 ℃) was added to each well for culture. \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518207795275,"sku":null,"price":1083.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/56247d0a27bf4f4eacba370062699fb4_f36bcb1e-8ce3-4677-b6ea-1a9baad52169.jpg?v=1755257710"},{"product_id":"hepatocarcinoma-like-organ-culture-medium-kit","title":"Organotial Human Liver Cancer Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) After sampling, the tissue must be placed in a sampling bottle containing pre-cooled (2-8°C) tissue preservation solution E and quickly transported to a clean laboratory for tissue processing and cell separation, and photographed and registered. \u003cbr\u003e(2) Prepare several culture dishes and add 4°C pre-cooled primary culture buffer B for use. \u003cbr\u003e(3) Disinfect the sampling bottle, place the tissue in the culture dish, wash it three times with primary culture buffer B, and use ophthalmic scissors or a scalpel to cut the tissue into tissue blocks of approximately 1-3 mm\u003csup\u003e3\u003c\/sup\u003e in volume. \u003cbr\u003e(4) Digest the tissue with human liver cancer primary tissue digestion solution C at 37°C for 10-20 minutes (observe the digestion progress at any time during the digestion process). \u003cbr\u003e(5) Take a small amount of liquid and observe it under a microscope. When a large number of single cells or cell clusters less than 70 μm are observed under the microscope, add three times the volume of primary culture buffer B to stop the digestion. \u003cbr\u003e(6) Filter using a 100 μm pore size mesh, collect the filtrate, concentrate and centrifuge at 300 g for 5 minutes, remove the supernatant, add primary culture buffer B and resuspend and centrifuge. \u003cbr\u003e(7) Matrigel calculation: After step 6, observe the volume of tissue collected, add 25 times the volume of tissue Matrigel (ABS9495) to resuspend and plate. \u003cbr\u003e(8) For a 24-well cell culture plate, dispense 25 μl of tissue-matrix gel mixture into each well for plating (operate at 4°C). \u003cbr\u003e(9) Place the plated culture plate in a 37°C incubator for 10-15 minutes to gel, add human liver cancer organoid culture medium A (return to room temperature) and culture. \u003cbr\u003e\u003cstrong\u003e2. Organoid subculture\u003c\/strong\u003e\u003cbr\u003e(1) Remove the culture medium with a pipette, add 1-2 ml of 4°C organoid subculture buffer G to each well and let it sit for 2 minutes. \u003cbr\u003e(2) Gently blow the matrix gel with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4℃ for 10 minutes. (Each 6-8 wells are a group)\u003cbr\u003e(3)a: When the number of organoids is insufficient or the volume is small: Centrifuge for 5 minutes and discard the supernatant, add an appropriate amount of organoid subculture buffer G, resuspend and transfer to a 1.5ml centrifuge tube, centrifuge at 300g for 5 minutes, discard the liquid and proceed to step 4. \u003cbr\u003e? ? ? ? ?b: When the number of organoids is large or the volume is large: Centrifuge for 5 minutes and discard the supernatant, add an appropriate amount of organoid subculture buffer D and digest for 2-3 minutes, add organoid subculture buffer G to stop digestion, centrifuge for 5 minutes and discard the mixed solution, add an appropriate amount of organoid subculture buffer G and resuspend and transfer to a 1.5ml centrifuge tube, centrifuge at 300g for 5 minutes, discard the liquid and proceed to step 4. \u003cbr\u003e(4) After the organoids are collected, add Matrigel to resuspend them. 25ul of Matrigel is spread on each well of a 24-well cell culture plate. Place it in the incubator for 10-15 minutes and then add 500ul of human liver cancer organoid culture medium A. \u003cbr\u003e\u003cstrong\u003e3. Organoid freezing\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette and add 1-2ml of 4℃ organoid subculture buffer G to each well and let it stand for 2 minutes. \u003cbr\u003e(2) Gently pipette the Matrigel and collect it in a 15ml centrifuge tube and let it stand at 4℃ for 10 minutes. (Each 6-8 wells are a group) \u003cbr\u003e(3) Centrifuge for 5 minutes and discard the supernatant. Add an appropriate amount of organoid subculture buffer G and resuspend again. Centrifuge at 300g for 5 minutes and discard the liquid. \u003cbr\u003e(4) Add an appropriate amount of organoid freezing medium F and gently pipette to resuspend. Taking a 24-well cell culture plate as an example: the density is 2 wells and freeze 1 tube. The volume of each tube is 1.4ml. \u003cbr\u003e(5) Label the cells, cool them down, and transfer them to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4. Organoid Recovery\u003c\/strong\u003e\u003cbr\u003e(1) Place 10 ml of Organoid Subculture Buffer G in a 15 ml centrifuge tube. \u003cbr\u003e(2) Remove the frozen organoid cells from the liquid nitrogen tank and quickly thaw them in a 37°C water bath. \u003cbr\u003e(3) During the thawing process in the water bath, gently shake the cryovial to ensure that the cryopreservative solution is completely thawed within 1-2 minutes. \u003cbr\u003e(4) Quickly transfer the dissolved organoid cells to a 15 ml centrifuge tube, gently pipette 6-8 times, centrifuge at 300 g for 5 minutes, then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of Organoid Subculture Buffer G, resuspend, transfer to a 1.5 ml centrifuge tube, and centrifuge at 300 g for 5 minutes. (5) Resuspend the matrix gel and spread 25ul matrix gel per well in a 24-well cell culture plate. Place it in the incubator for 10-15 minutes to gel, and add 500ul human liver cancer organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit Composition: \u003cbr\u003e\u003ctable style=\"width: 62.0236%; height: 154px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; text-align: center; height: 22px;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; text-align: center; height: 22px;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px;\"\u003eHuman Hepatocellular Carcinoma Organoid Culture Medium A\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px;\"\u003ePrimary Culture Buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px;\"\u003eHuman Liver Cancer Primary Tissue Digestion Solution C\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px;\"\u003eOrganoid Passaging Digestion Solution D\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 38.5424%; height: Tissue Preservation Solution E\u0026lt;\/td\u0026gt;\u0026lt;td style=\" width: height:\u003e100ml\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px;\"\u003eOrganoid Cryopreservation Solution F\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 38.5424%;\"\u003eOrganoid Subculture Buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 4℃, valid for 3 months; store at -20℃, valid for 1 year. See label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41683107774539,"sku":null,"price":1032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_dba8b0c4-acdc-4615-b820-3a2f5a42df58.png?v=1770720294"},{"product_id":"mouse-pancreas-like-organ-culture-medium-kit","title":"Mouse Pancreatic Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The collected tissue must be placed in a sampling bottle of pre-cooled (2-8 °C) tissue preservation solution E, and quickly transported to a clean laboratory for tissue processing and cell separation, photographed and registered information.\u003cbr\u003e(2) Prepare several petri dishes, and add primary culture buffer B pre-cooled at 4 °C for later use.\u003cbr\u003e(3) Sterilize the sampling bottle, put the tissue in a petri dish, wash it three times with primary culture buffer B, and cut the tissue into a volume of about 1-3mm with ophthalmic scissors or scalpel\u003csup\u003e3\u003c\/sup\u003eThe tissue block.\u003cbr\u003e(4) The tissues were digested with mouse normal pancreatic primary tissue digestive juice C, and digested by shaking at 37 ℃ for 10-20min (the digestion situation was observed at any time during digestion).\u003cbr\u003e(5) Take a small amount of liquid and observe it under a microscope. After more single cells or cell clusters below 70um are observed under the microscope, add triploid volume primary culture buffer B to terminate digestion.\u003cbr\u003e(6) Filter with a sieve with a pore size of 100um, collect the filtrate, enrich and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B and re-suspend and centrifuge.\u003cbr\u003e(7) Matrigel calculation: After step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend and plate.\u003cbr\u003e(8) Take a 24-well cell culture plate as an example. Each well is dispensed with 25ul tissue matrigel mixture for plating (operation at 4 °C).\u003cbr\u003e(9) Put the laid culture plate into a 37 ℃ incubator for 10-15 minutes to gel, and add mouse normal pancreatic organoid medium A (restored to room temperature) for culture.\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer G to each well and leave for 2min.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) a: When the number of organoids is insufficient or the volume is small: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture buffer G, resuspend it and transfer it into a 1.5 ml centrifuge tube, centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture juice D for 2-3 minutes, add organoid subculture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixed solution, add an appropriate amount of organoid subculture buffer G to resuspend and transfer into a 1.5 ml centrifuge tube, centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003e(4) After organoid collection, add matrigel for resuspension, spread 25ul of matrigel per well in a 24-well cell culture plate, and place it in an incubator for 10-15min to add 500ul of mouse normal pancreatic organoid medium A.\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer G to each well and leave for 2min.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture buffer G to resuspend again, and centrifuge 300g for 5 minutes to discard the liquid.\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take a 24-well cell culture plate as an example: the density is 2 wells for cryopreservation in 1 tube, and the volume of each tube is 1.4 ml.\u003cbr\u003e(5) Make good marking information, carry out program cooling, and then move it into liquid nitrogen for long-term storage.\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organoid subculture buffer G in a 15ml centrifuge tube.\u003cbr\u003e(2) Take out the frozen organoid cells from the liquid nitrogen tank and quickly thaw them in a 37 ℃ water bath.\u003cbr\u003e(3) During the water bath melting process, the cryotube needs to be gently shaken to ensure that the cryopreservation solution is completely melted within 1-2 minutes.\u003cbr\u003e(4) Quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently pipette 6-8 times with a pipette, centrifuge at 300g for 5min, then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer G, resuspend it, transfer it into a 1.5 ml centrifuge tube with 300g and centrifuge for 5 minutes.\u003cbr\u003e(5) Resuspend Matrigel, spread 25ul Matrigel per well in a 24-well cell culture plate, place it in an incubator for 10-15min to gel, and add 500ul mouse normal pancreatic organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Pancreatic Organoid Culture Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003eKit composition:\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eMouse normal pancreatic organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eMouse normal pancreatic primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid Cryopreservation Solution F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003eOrganoid Subculture Buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 4 °C, shelf life is 3 months; -20 °C, shelf life 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41683107807307,"sku":null,"price":1032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_47bfcc6e-1e4a-4af0-9c66-2044f0bf263f.png?v=1770720291"},{"product_id":"human-intestinal-organoid-culture-medium-kit","title":"Human Intestinal Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The collected tissue must be placed in a sampling bottle of pre-cooled (2-8 °C) tissue preservation solution E, and quickly transported to a clean laboratory for tissue processing and cell separation, photographed and registered information.\u003cbr\u003e(2) Prepare several petri dishes, and add primary culture buffer B pre-cooled at 4 °C for later use.\u003cbr\u003e(3) Disinfect the sampling bottle, put the tissue in a petri dish, wash it three times with primary culture buffer B, remove impurities, and remove the intestinal mucosa of small intestine tissue with ophthalmic scissors or scalpel, and cut it into a volume of about 1-3mm\u003csup\u003e3\u003c\/sup\u003eThe tissue block.\u003cbr\u003e(4) The tissue was digested with human normal intestinal primary tissue digestive juice C, and digested by shaking at 4 ℃ for 10-20min (observe the digestion at any time during the digestion process).\u003cbr\u003e(5) Take a small amount of liquid and observe it under a microscope. After more single cells or cell clusters below 70um are observed under the microscope, add triploid volume primary culture buffer B to terminate digestion.\u003cbr\u003e(6) Filter with a sieve with a pore size of 100um, collect the filtrate, enrich and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B and re-suspend and centrifuge.\u003cbr\u003e(7) Matrigel calculation: After step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend and plate.\u003cbr\u003e(8) Take a 24-well cell culture plate as an example. Each well is dispensed with 25ul tissue matrigel mixture for plating (operation at 4 °C).\u003cbr\u003e(9) Put the laid culture plate into a 37 ℃ incubator for 10-15 minutes to form a gel, and add human normal intestinal organoid culture medium A (restored to room temperature) for culture.\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer G to each well and place for 2min.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) a: When the number of organoids is insufficient or the volume is small: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture buffer G, resuspend it and transfer it into a 1.5 ml centrifuge tube, centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture juice D for 2-3 minutes, add organoid subculture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixed solution, add an appropriate amount of organoid subculture buffer G to resuspend and transfer into a 1.5 ml centrifuge tube, centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003e(4) After organoid collection, add Matrigel for resuspension, spread 25ul of Matrigel per well in a 24-well cell culture plate, place it in an incubator for 10-15min, and add 500ul of human normal intestinal organoid culture medium A.\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer G to each well and place for 2min.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture buffer G to resuspend again, and centrifuge 300g for 5 minutes to discard the liquid.\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take a 24-well cell culture plate as an example: the density is 2 wells for cryopreservation in 1 tube, and the volume of each tube is 1.4 ml.\u003cbr\u003e(5) Make good marking information, carry out program cooling, and then move it into liquid nitrogen for long-term storage.\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of organoid subculture buffer G in a 15ml centrifuge tube.\u003cbr\u003e(2) Take out the frozen organoid cells from the liquid nitrogen tank and quickly thaw them in a 37 ℃ water bath.\u003cbr\u003e(3) During the water bath melting process, the cryotube needs to be gently shaken to ensure that the cryopreservation solution is completely melted within 1-2 minutes.\u003cbr\u003e(4) Quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently pipette 6-8 times with a pipette, centrifuge at 300g for 5min, then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer G, resuspend it, transfer it into a 1.5 ml centrifuge tube with 300g and centrifuge for 5 minutes.\u003cbr\u003e(5) Resuspend Matrigel, spread 25ul Matrigel per well in a 24-well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul human normal intestinal organoid culture medium A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman normal intestinal organoid culture medium is a culture medium that promotes the formation and expansion of human normal intestinal organoids in vitro. The product is a sterile liquid mixing system containing amino acids, vitamins, organic and inorganic compounds and growth factors essential to maintain the growth of target cells. The unique formula of this medium can provide a suitable nutritional environment required by cells and promote the growth and expansion of human normal intestinal organoids in vitro.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003eTry\u003cstrong\u003e\u003cstrong\u003eKit composition:\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 72.89%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eHuman normal intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eHuman normal intestinal primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eOrganoid Cryopreservation Solution F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003eOrganoid Subculture Buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 4 °C, shelf life is 3 months; -20 °C, shelf life 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41683107840075,"sku":null,"price":1032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_54203a7f-f200-4bf8-9734-a484d49b41c8.png?v=1770720288"},{"product_id":"endometrial-ancer-like-organ-culture-medium","title":"Human Endometrial carcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Primary\u003c\/strong\u003e\u003cbr\u003e(1) The collected tissue is placed in a sampling bottle of pre-cooled (2-8 °C) tissue preservation solution, and quickly transferred to a clean laboratory for tissue processing and cell separation, taking photos and registering information.\u003cbr\u003e(2) Prepare several petri dishes, and add 4 ℃ pre-cooled human endometrial cancer organoid tissue primary culture buffer # abs9731 for later use.\u003cbr\u003e(3) Sterilize the sampling bottle, put the tissue into a petri dish, wash it three times with the primary culture buffer of human endometrial cancer organoid tissue, and cut the tissue into a volume of about 1-3mm with ophthalmic scissors or scalpel\u003csup\u003e3\u003c\/sup\u003eThe tissue block.\u003cbr\u003e(4) The tissues were digested with human endometrial cancer primary tissue digestive juice # abs9522, and digested by shaking at 37 ℃ for 10-20min (observe the digestion at any time during the digestion process).\u003cbr\u003e(5) Take a small amount of liquid and observe it under a microscope. After more single cells or cell clusters below 70um are observed under the microscope, add triploid human endometrial carcinoma organoid tissue primary culture buffer to stop digestion.\u003cbr\u003e(6) Filter with a sieve with a pore size of 100um, collect the filtrate, enrich and centrifuge at 300g for 5 minutes, remove the supernatant, add human endometrial cancer organoid tissue primary culture buffer and re-suspend and centrifuge.\u003cbr\u003e(7) Matrigel calculation: After step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel # abs9495 to resuspend and plate.\u003cbr\u003e(8) Take a 24-well cell culture plate as an example. Each well is dispensed with 25ul tissue matrigel mixture for plating (operation at 4 °C).\u003cbr\u003e(9) Put the laid culture plate into a 37 ℃ incubator for 10-15 minutes to gel, and add human endometrial cancer organoid culture medium (restored to room temperature) for culture.\u003cbr\u003e\u003cstrong\u003e2. Organoid subculture\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer # abs9730 to each well for 2min.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) a: When the number of organoids is insufficient or the volume is small: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of human endometrial cancer organoid subculture buffer to resuspend and transfer it into a 1.5 ml centrifuge tube, centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003eb: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid subculture juice # abs9520 for 2-3 minutes, add human endometrial cancer organoid subculture buffer to terminate digestion, centrifuge for 5 minutes to discard the mixed solution, add an appropriate amount of human endometrial cancer organoid subculture buffer to resuspend and transfer into a 1.5 ml centrifuge tube, and centrifuge 300g for 5 minutes to discard the liquid for step 4.\u003cbr\u003e(4) After organoid collection, add Matrigel for resuspension, spread 25ul of Matrigel per well in a 24-well cell culture plate, and place it in an incubator for 10-15min to add 500ul of human endometrial cancer organoid culture medium.\u003cbr\u003e\u003cstrong\u003e3. Organoid cryopreservation\u003c\/strong\u003e\u003cbr\u003e(1) Aspirate the culture medium with a pipette gun, add 1-2ml of 4 ℃ organoid subculture buffer to each well and place it for 2 minutes.\u003cbr\u003e(2) Gently blow the matrigel with a pipette gun, collect it in a 15ml centrifuge tube, and let it stand at 4 °C for 10 minutes. (groups of 6-8 wells)\u003cbr\u003e(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of human endometrial cancer organoid subculture buffer to resuspend again, and centrifuge 300g for 5 minutes to discard the liquid.\u003cbr\u003e(4) Add an appropriate amount of organoid cryopreservation solution # abs9519, gently blow and resuspend, take a 24-well cell culture plate as an example: the density is 2 wells and cryopreserve 1 tube, each tube has a volume of 1.4 ml.\u003cbr\u003e(5) Make good marking information, carry out program cooling, and then move it into liquid nitrogen for long-term storage.\u003cbr\u003e\u003cstrong\u003e4. Organoid resuscitation\u003c\/strong\u003e\u003cbr\u003e(1) Take 10ml of human endometrial carcinoma organoid subculture buffer in a 15ml centrifuge tube.\u003cbr\u003e(2) Take out the frozen organoid cells from the liquid nitrogen tank and quickly thaw them in a 37 ℃ water bath.\u003cbr\u003e(3) During the water bath melting process, the cryotube needs to be gently shaken to ensure that the cryopreservation solution is completely melted within 1-2 minutes.\u003cbr\u003e(4) Quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently pipette 6-8 times with a pipette, centrifuge at 300g for 5min, then remove the supernatant and collect the organoid cell pellet. Add appropriate amount of human endometrial cancer organoid subculture buffer, resuspend, transfer into 1.5 ml centrifuge tube 300g and centrifuge for 5min.\u003cbr\u003e(5) Matrigel is resuspended, 25ul of Matrigel per well is spread in a 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500ul of human endometrial cancer organoid culture medium is added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 4 °C, shelf life is 3 months; Store at-20 °C, shelf life 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":41683108692043,"sku":null,"price":635.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":41683108724811,"sku":null,"price":2032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_7da2539b-5fd1-4c80-9f42-3b3b5caf8479.jpg?v=1755048478"},{"product_id":"celltiter-organoid-viability-assay-kit","title":"2D\/3D\/Organoid ATP Viability Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Preparation of organoid:\u003c\/strong\u003e\u003cbr\u003eUse 96-well plate suitable for chemiluminescence detection (bottom and lid are transparent, well wall is impermeable, recommended item number: abs7242), inoculate 5ul-10ul organoid matrigel suspension per well, put it in the incubator for more than half an hour, add 100ul organoid culture medium after coagulation (if 384-well plate is used, inoculate 2.5 μl-4μl organoid matrigel suspension per well, depending on different types of 384-well plate), and ensure that the number of cells per well is within 50,000 (if 384-well plate is used). If desired, drugs can be added to treat organoids. In addition, if necessary, the concentration gradient of organoids can also be set to subsequently determine the effectiveness of the use of the kit.\u003cbr\u003e\u003cstrong\u003e2. Reagent preparation:\u003c\/strong\u003e\u003cbr\u003eAdd 1 tube of freeze-dried powder to 1 tube of 10ml buffer, mix well and protect from light for later use.\u003cbr\u003e\u003cstrong\u003eNote: Use as soon as possible after dissolving,-80 ℃, protected from light, valid for one month\u003c\/strong\u003e\u003cbr\u003e(1) Dissolve the Organoid ATP luminescence detection reagent, and dispense the reagent appropriately if necessary.\u003cbr\u003e(2) According to the amount of 100 μl per well of 96-well plate (25 μl per well of 384-well plate), take an appropriate amount of Organoid ATP luminescence detection reagent, and equilibrate to room temperature.\u003cbr\u003e\u003cstrong\u003e3. Organoid viability detection:\u003c\/strong\u003e\u003cbr\u003e(1) Take out the organoid culture plate and equilibrate it at room temperature for 10 minutes (usually not more than 30 minutes).\u003cbr\u003e(2) The organoid medium in each well of the 96-well plate was removed, and 100 μl of Organoid ATP luminescence detection reagent was added to each well (25 μl per well of the 384-well plate).\u003cbr\u003eNote: No removal of Matrigel is required.\u003cbr\u003e(3) Violent oscillation at room temperature (microplate constant temperature oscillator, recommended item number: abs72034) for 5 minutes to fully lyse the organoids.  \u003cbr\u003e(4) Incubate at room temperature (about 25 °C) for 10 minutes to stabilize the luminescence signal.\u003cbr\u003e(5) Use a multifunctional microplate reader with the function of detecting chemiluminescence for chemiluminescence detection. Please set the corresponding parameters according to the requirements of the instrument. The detection time of each hole is generally 0.25-1 second or longer. The specific adjustment needs to be made according to the detection sensitivity of the instrument.\u003cbr\u003e(6) Calculate the relative viability of organoids directly from the chemiluminescence readings, or calculate the amount of ATP according to the ATP standard curve to calculate the relative viability of organoids.\u003cbr\u003eNote: The detection effect varies depending on the type of organoid. For some organoids with particularly high ATP content, there may not be a linear correlation after the number of cells reaches 50,000, but the chemiluminescence reading will continue to increase.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eATP; ATP detection; CTG; ATPlite; Luminescent cell viability detection kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is an ATP detection kit specially developed for the detection of organoid activity. Its luminescence technology principle is that luciferase uses luciferin, adenosine triphosphate (ATP) and O\u003csub\u003e2\u003c\/sub\u003eIs the substrate, in Mg\u003csup\u003e2+\u003c\/sup\u003eWhen present, it can convert chemical energy into light energy. ATP is not only an essential substrate for luciferase catalyzed luminescence, but also an energy source for all biological life activities. In the luminescence reaction catalyzed by luciferase, the concentration of ATP has a linear relationship with the luminescence intensity within a certain concentration range.\u003cbr\u003eThis kit uses bioluminescent method, uses Firefly luciferase (Firefly luciferase) to catalyze the conversion of substrate-luciferin, and efficiently uses the energy of ATP to emit photons. The luminescence signal is proportional to the amount of ATP present, and ATP is directly proportional to the number of cells present in the organoid.\u003cbr\u003e\u003cbr\u003eDesigned for multi-well plates, this kit is ideal for automated high-throughput screening (HTS), organoid proliferation and toxicity analysis. The homogenization detection step is to directly add a single reagent to the well plate containing organoid culture medium without removing the matrigel.\u003cbr\u003e\u003cbr\u003eThe \"spike-mix-detect\" protocol of homogeneous detection allows organoid lysis and the luminescence signal produced to be proportional to the amount of ATP present, which is directly proportional to the number of cells in the organoid. The unique homogeneous detection protocol avoids the errors that may be introduced by ATP detection methods that require multiple steps.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003eProduct composition:\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 84.7969%; height: 66px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 35.816%; height: 22px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 17.8987%; height: 22px; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 16.3766%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 22.8095%; height: 22px; text-align: center;\"\u003ePreservation method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 35.816%; height: 22px; text-align: center;\"\u003eOrganoid ATP Fluorescent dye\u003c\/td\u003e\n\u003ctd style=\"width: 17.8987%; height: 22px; text-align: center;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 16.3766%; height: 22px; text-align: center;\"\u003e10 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 22.8095%; height: 22px; text-align: center;\"\u003e2-8 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 35.816%; height: 22px; text-align: center;\"\u003eOrganoid ATP dye buffer\u003c\/td\u003e\n\u003ctd style=\"width: 17.8987%; height: 22px; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 16.3766%; height: 22px; text-align: center;\"\u003e10 × 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 22.8095%; height: 22px; text-align: center;\"\u003e2-8 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003eNote: The product is recommended for immediate use\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eProduct Features:\u003c\/strong\u003e\u003cbr\u003e(1) Simplified organoid activity detection steps: the homogeneous \"sample addition-mixing-detection\" scheme reduces the operation steps required for other similar detections.\u003cbr\u003e(2) Less amount of organoids: the number of cells below the low detection limit of commonly used colorimetric and fluorescent methods can be accurately detected. The number of cells required per detection reaction is reduced.\u003cbr\u003e(3) Obtain the results quickly: the data can be obtained 10 minutes after adding the reagent.\u003cbr\u003e(4) You can choose your own detection scheme: it can be used for many types of multi-well plate operations. The data can be recorded with a luminescence detector or CCD imaging equipment.\u003cbr\u003e(5) The culture plate can be continuously processed: the luminescence signal is stable, and the samples can be processed in batches.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. The activity of luciferase is sensitive to temperature, so the organoids and detection reagents need to be balanced to room temperature before the reaction before measurement. Please mix the test reagents well before use.\u003cbr\u003e2. The detection reagent of this kit contains luciferase, and repeated freezing and thawing will cause its gradual inactivation. In order to achieve good use effect, it can be appropriately packaged and stored after the first dissolution, but it should be noted that the packaged container should not be contaminated by ATP.\u003cbr\u003e3. When the solvent content of the drug to be tested is high, it may interfere with the luciferase reaction, thus affecting the chemiluminescence signal. Solvent interference can be eliminated by setting organoid culture medium control wells containing solvent.\u003cbr\u003e4. White or black 96-well plates or 384-well plates suitable for organoid culture must be used for testing. If a plain clear 96-well plate or 384-well plate is used, adjacent wells will interfere with each other.\u003cbr\u003e5. This product is limited to scientific research by professionals. It shall not be used for clinical diagnosis or treatment, food or medicine, or stored in ordinary houses.\u003cbr\u003e6. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2-8 ℃, protected from light, valid for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":41683109052491,"sku":null,"price":667.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":41683109085259,"sku":null,"price":143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d648da7b-0136-4a65-9674-45d97ce6764f.png?v=1770717335"},{"product_id":"porcine-intestine-organoid-culture-kit","title":"Porcine Intestine Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eTake a 24-well plate as an example\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. Sample preprocessing\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: primary culture buffer B (pre-cooled at 4 ℃), tissue preservation solution E, sampling tube, tissue transport box and ice pack.\u003cbr\u003e2. Sample sampling and transportation: The tissue should be put into tissue preservation solution E and primary culture buffer B within 30 minutes of isolation, washed 3-5 times, the blood on the tissue surface should be washed clean, put into tissue preservation solution E, and the sampling tube should be stored and transported at a low temperature of 4 ℃ (within 72 hours).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Primary culture of organoids\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cbr\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), tweezers (10 cm), pointed ophthalmic surgical scissors\/blade, disposable 60 mm culture dish, 1.5 mL\/15 mL\/50 mL EP tube, 100 um cell strainer, 3 mL pasteurization pipette\/1000 uL pipette gun, 24-well cell culture plate, metal ice box.\u003cbr\u003e(2) Kit reagents: pig normal intestinal organoid culture medium A (room temperature or 37 ℃), primary culture buffer B (4 ℃), and primary tissue digestion juice C (37 ℃).\u003cbr\u003e2. Organoid construction:\u003cbr\u003e(1) It is recommended that the tissue after sampling materials be stored and transported at 2-8 ℃, and quickly transported to a clean laboratory for the experimental process of pig normal intestinal organoid construction, organize photos and register detailed information.\u003cbr\u003e(2) After the sampling tube is disinfected, take out the tissue on the ultra-clean table, put it in a culture dish, add primary culture buffer B, blow and clean with a 3 mL pasteurization pipette or a 1000 uL pipette gun, and repeat the cleaning operation 3 times or more.\u003cbr\u003e(3) Remove tissue impurities with ophthalmic scissors or surgical blade, transfer them to 1.5 mL EP tube with tweezers, clean them with primary buffer for three times, scrape the villi, and further mechanically dissociate the tissue with ophthalmic scissors into a volume of about 1-3 mm\u003csup\u003e3\u003c\/sup\u003eTransfer the tissue block to a 15 mL EP tube, add 5 mL of pig normal intestinal primary tissue digestive juice C at 4 ℃ and shake for 15-25 min. During the digestion process, the tissue digestion was observed under a microscope every 10 minutes, and a small amount of digestive juice was taken to observe under a microscope. After more cell clusters or single cells below 70 um were observed, the next operation was carried out.\u003cbr\u003e(4) The tissue is filtered through a cell screen with a pore size of 100 μm, collecting the filtrate, adding 3 times the volume of primary culture buffer B for rinse to terminate the digestion, enriching 300 g and centrifuging for 5 minutes, and discarding the supernatant; If the cell pellet contains red blood cells, add 1-2 mL of red blood cell lysate for 1-2 min, dilute to 10 mL, enrich 300 g and centrifuge for 5 min, and then discard the supernatant.\u003cbr\u003e(5) Observe the volume of cell pellets collected by centrifugation, add 25 times the volume of Matrigel to resuspend, form a 3D culture space structure, and avoid bubbles during resuspension. The volume of cell pellet is shown in the figure below. Add 200 uL, 150 uL, 100 uL and 50 uL of Matrigel according to the cell pellet volume as shown in the figure.\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/318431bcb65e4dc9a8be5eed4bcf2962.png\" alt=\"\" width=\"250\" height=\"127\"\u003e\u003cbr\u003e\u003cp style=\"text-align: justify;\"\u003eExample: 24-well cell culture plates were dispensed according to 25 uL-30 uL\/well,\u003cstrong\u003eMatrigel\u003c\/strong\u003e\u003cstrong\u003ewhole course\u003c\/strong\u003e\u003cstrong\u003eMaintain at 0-4\u003c\/strong\u003e\u003cstrong\u003e?\u003c\/strong\u003e\u003cstrong\u003eOperation at ℃\u003c\/strong\u003e。 Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of porcine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Organoid subculture\u003cbr\u003e\u003c\/strong\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), 1.5 mL\/15 mL EP tube, 3 mL pasteurization pipette\/1000 uL pipette gun, 24-well cell culture plate, metal ice box.\u003cbr\u003e(2) Kit reagents: pig normal intestinal organoid culture medium A (room temperature or 37 ℃), organoid subculture digestion juice D (37 ℃), organoid subculture buffer G (4 ℃).\u003cbr\u003e2. Organoid passage:\u003cbr\u003e(1) Select suitable organoids for passage, generally for about one week of growth. More than 20 organoids, or organoids with a size of 100-200 um, can be seen under a microscope of 10X. Aspirate the medium, add an equal volume of organoid subculture buffer G to each well, gently blow the Matrigel with a pipette gun, collect it in a 15 mL EP tube, transfer it to an EP tube every 6-8 wells, and stand at 4 ℃ for 10-15 minutes.\u003cbr\u003e(2) Decide whether digestion and passage are needed according to the growth of organoids.\u003cstrong\u003eNote:\u003c\/strong\u003eAfter centrifugation, if there is little sediment at the bottom of the tube, no cells are seen, and the matrigel is not stratified, it can be resuspended again, the centrifugal force can be increased, and centrifuged again.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eA: When the number of organoids is insufficient or the volume is small, centrifuge at 300 g for 5 min and discard the supernatant.\u003cbr\u003eb: When the number of organoids is large or the volume is large, centrifuge at 300 g for 5 minutes to discard the supernatant, and select digestive juice for digestion.\u003cbr\u003eDigestive juice digestion: Add 1-2 mL of organoid passage digestive juice D, blow the cell pellet away, digest at room temperature for 2-4 min, pipetting every minute, and observe under a microscope until the digestion reaches the state (Figure A-B). Organoid subculture buffer G was added 3 times the volume of organoid digestion fluid to terminate digestion, and the supernatant was discarded by centrifugation at 300 g for 5 min.\u003c\/p\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240517\/f1d0734b815a418d9221d3933302fddc.png\" alt=\"\" width=\"428\" height=\"134\"\u003e\u003cbr\u003e(3) Observe the volume of organoid precipitate collected by centrifugation. If there is little precipitate, reserve 1 time the precipitate volume of supernatant for dispensing. If a lot of precipitate supernatant can be aspirated. Add 25 times the matrigel volume of organoid precipitate to resuspend organoids. Example: 24-well cell culture plates were dispensed according to 25 uL-30 uL\/well,\u003cstrong\u003eThe Matrigel is maintained at 0-4 ℃ throughout the process\u003c\/strong\u003e。 Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of porcine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Organoid cryopreservation\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be prepared by yourself: 15 mL EP tube, cell cryopreservation tube, program cooling box, pipette gun.\u003cbr\u003e(2) Kit reagents: organoid subculture buffer G (4 ℃), organoid cryopreservation solution F.\u003cbr\u003e2. Organoid cryopreservation:\u003cbr\u003eOrganoids that are not in use temporarily should be frozen and stored in a low temperature environment.\u003cbr\u003e(1) Aspirate the medium, add an equal volume of organoid subculture buffer G to each well, gently blow the matrigel with a pipette gun, collect it in a 15 mLEP tube, transfer it to an EP tube every 6-8 wells, and stand at 4 ℃ for 10-15 minutes. Centrifuge at 300 g for 5 minutes to discard the supernatant, add 2 mL of organoid cryopreservation solution F every three wells, mix well by gently pipetting, and transfer to cell cryopreservation tubes, each tube containing 1 mL.\u003cbr\u003e(2) Make the mark information, put it in the program cooling box, move it to the-80 ℃ refrigerator, and put it in the liquid nitrogen tank for storage after 48 hours. Or put it in a 4 ℃ refrigerator for 40 minutes, put it in a-20 ℃ refrigerator for 2 hours, move it to a-80 ℃ refrigerator, and after 48 hours, put it in a liquid nitrogen tank for storage.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e5. Organoid resuscitation culture\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), 15 mL EP tube, water bath, pipette gun, 24-well cell culture plate, ice box.\u003cbr\u003e(2) Kit reagents: organoid subculture buffer G (4 ℃), pig normal intestinal organoid culture medium A.\u003cbr\u003e2. Organoid resuscitation culture:\u003cbr\u003e(1) Take out the frozen organoids from the low temperature environment and quickly put them in a 37 ℃ water bath to thaw. During the thawing process of the water bath, the cryopreservation tube should be gently shaken to ensure that the cryopreservation solution is completely melted in a short time. The thawed organoids were quickly transferred to 15 mLEP tubes, gently pipetted 6-8 times with a pipette gun, and centrifuged at 300 g for 5 minutes to discard the supernatant; Add an appropriate amount of passage buffer G to resuspend, transfer it into a 1.5 mL EP tube at 300 g and centrifuge for 5 minutes, and discard the supernatant.\u003cbr\u003e(2) Add 100 uL of Matrigel to each cryopreservation tube for resuspension, dispense 24-well cell culture plates according to 25 uL-30 uL\/well, and maintain the Matrigel at 0-4 ℃ throughout the process. Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of porcine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003eKit composition:\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePorcine normal intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePorcine normal intestinal primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid Cryopreservation Solution F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003eOrganoid Subculture Buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Pig normal intestinal organoid culture medium A can be stored at 4 ℃ for 3 months, and stored at 4 ℃ after receiving the goods. It is recommended to use it within 1 month. It is recommended to store it at-20 ℃ for a long time to avoid repeated freezing and thawing more than 2 times\u003cstrong\u003e\u003cstrong\u003e。\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e2. Tissue preservation solution E and pig normal intestinal primary tissue digestive juice C contain nutrients to maintain cell activity. In order to maintain the activity of reagent nutrients, it is not recommended to store them at-20 ℃ for a long time to avoid repeated freezing and thawing more than twice.\u003cbr\u003e3. Under ambient conditions of 2-8 °C, matrigel was thawed overnight. When using Matrigel, keep it in an ice box to prevent premature setting. Matrigel formed a gel within 20 min at 37 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ℃, valid for 3 months; Store at-20 ℃, shelf life is 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41683109216331,"sku":null,"price":1032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_dba8b0c4-acdc-4615-b820-3a2f5a42df58.png?v=1770720294"},{"product_id":"beef-intesine-organoid-culture-kit","title":"Beef Intesine Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eTake a 24-well plate as an example\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. Sample preprocessing\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials: primary culture buffer B (pre-cooled at 4 ℃), tissue preservation solution E, sampling tube, tissue transport box and ice pack.\u003cbr\u003e2. Sample sampling and transportation: The tissue should be put into tissue preservation solution E and primary culture buffer B within 30 minutes of isolation, washed 3-5 times, the blood on the tissue surface should be washed clean, put into tissue preservation solution E, and the sampling tube should be stored and transported at a low temperature of 4 ℃ (within 72 hours).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Primary culture of organoids\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cbr\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), tweezers (10 cm), pointed ophthalmic surgical scissors\/blade, disposable 60 mm culture dish, 1.5 mL\/15 mL\/50 mL EP tube, 100 um cell strainer, 3 mL pasteurization pipette\/1000 uL pipette gun, 24-well cell culture plate, metal ice box.\u003cbr\u003e(2) Kit reagents: bovine normal intestinal organoid culture medium A (room temperature or 37 ℃), primary culture buffer B (4 ℃), and primary tissue digestion juice C (37 ℃).\u003cbr\u003e2. Organoid construction:\u003cbr\u003e(1) It is recommended that the tissue after sampling materials should be stored and transported at 2-8 ℃, and quickly transported to a clean laboratory for the experimental process of bovine normal intestinal organoid construction, and the tissue should take photos and register detailed information.\u003cbr\u003e(2) After the sampling tube is disinfected, take out the tissue on the ultra-clean table, put it in a culture dish, add primary culture buffer B, blow and clean with a 3 mL pasteurization pipette or a 1000 uL pipette gun, and repeat the cleaning operation 3 times or more.\u003cbr\u003e(3) Remove tissue impurities with ophthalmic scissors or surgical blade, transfer them to 1.5 mL EP tube with tweezers, clean them with primary buffer for three times, scrape the villi, and further mechanically dissociate the tissue with ophthalmic scissors into a volume of about 1-3 mm\u003csup\u003e3\u003c\/sup\u003eTransfer the tissue block to a 15 mL EP tube, add 5 mL of bovine normal intestinal primary tissue digestive juice C at 4 ℃ and shake for 15-25 min. During the digestion process, the tissue digestion was observed under a microscope every 10 minutes, and a small amount of digestive juice was taken to observe under a microscope. After more cell clusters or single cells below 70 um were observed, the next operation was carried out.\u003cbr\u003e(4) The digested tissue is filtered through a cell screen with a pore size of 100 um, the filtrate is collected, 3 times the volume of primary culture buffer B is added to rinse to terminate the digestion, and the supernatant is discarded after enrichment and centrifugation at 300 g for 5 minutes; If the cell pellet contains red blood cells, add 1-2 mL of red blood cell lysate for 1-2 min, dilute to 10 mL, enrich 300 g and centrifuge for 5 min, and then discard the supernatant.\u003cbr\u003e(5) Observe the volume of cell pellets collected by centrifugation, add 25 times the volume of Matrigel to resuspend, form a 3D culture space structure, and avoid bubbles during resuspension. The volume of cell pellet is shown in the figure below. Add 200 uL, 150 uL, 100 uL and 50 uL of Matrigel according to the cell pellet volume as shown in the figure.\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/318431bcb65e4dc9a8be5eed4bcf2962.png\" alt=\"\" width=\"250\" height=\"127\"\u003e\u003cbr\u003e\u003cp style=\"text-align: justify;\"\u003eExample: 24-well cell culture plates were dispensed according to 25 uL-30 uL\/well,\u003cstrong\u003eMatrigel\u003c\/strong\u003e\u003cstrong\u003ewhole course\u003c\/strong\u003e\u003cstrong\u003eMaintain at 0-4\u003c\/strong\u003e\u003cstrong\u003e?\u003c\/strong\u003e\u003cstrong\u003eOperation at ℃\u003c\/strong\u003e。 Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of bovine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Organoid subculture\u003cbr\u003e\u003c\/strong\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), 1.5 mL\/15 mL EP tube, 3 mL pasteurization pipette\/1000 uL pipette gun, 24-well cell culture plate, metal ice box.\u003cbr\u003e(2) Kit reagents: bovine normal intestinal organoid culture medium A (room temperature or 37 ℃), organoid subculture digestion juice D (37 ℃), and organoid subculture buffer G (4 ℃).\u003cbr\u003e2. Organoid passage:\u003cbr\u003e(1) Select suitable organoids for passage, generally for about one week of growth. More than 20 organoids, or organoids with a size of 100-200 um, can be seen under a microscope of 10X. Aspirate the medium, add an equal volume of organoid subculture buffer G to each well, gently blow the Matrigel with a pipette gun, collect it in a 15 mL EP tube, transfer it to an EP tube every 6-8 wells, and stand at 4 ℃ for 10-15 minutes.\u003cbr\u003e(2) Decide whether digestion and passage are needed according to the growth of organoids.\u003cstrong\u003eNote:\u003c\/strong\u003eAfter centrifugation, if there is little sediment at the bottom of the tube, no cells are seen, and the matrigel is not stratified, it can be resuspended again, the centrifugal force can be increased, and centrifuged again.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eA: When the number of organoids is insufficient or the volume is small, centrifuge at 300 g for 5 min and discard the supernatant.\u003cbr\u003eb: When the number of organoids is large or the volume is large, centrifuge at 300 g for 5 minutes to discard the supernatant, and select digestive juice for digestion.\u003cbr\u003eDigestive juice digestion: Add 1-2 mL of organoid passage digestive juice D, blow the cell pellet away, digest at room temperature for 2-4 min, pipetting every minute, and observe under a microscope until the digestion reaches the state (Figure A-B). Organoid subculture buffer G was added 3 times the volume of organoid digestion fluid to terminate digestion, and the supernatant was discarded by centrifugation at 300 g for 5 min.\u003cbr\u003eMechanical digestion: Add 1 mL of organoid subculture buffer G, pipetting 1 mL of gun tip for 20-30 times until pipetting reaches the state (Figure C-D), and then stop. Add 10 mL of organoid subculture buffer G, centrifuge at 300 g for 5 minutes, and discard the supernatant.\u003c\/p\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240528\/e9b8ffea30f94525aa3755564b55f11b.png\" alt=\"\" width=\"416\" height=\"239\"\u003e\u003cbr\u003e(3) Observe the volume of organoid precipitate collected by centrifugation. If there is little precipitate, reserve 1 time the precipitate volume of supernatant for dispensing. If a lot of precipitate supernatant can be aspirated. Add 25 times the volume of matrigel of organoid precipitate to resuspend organoids. Example: 24-well cell culture plates were dispensed according to 25 uL-30 uL\/well,\u003cstrong\u003eThe Matrigel is maintained at 0-4 ℃ throughout the process\u003c\/strong\u003e。 Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of bovine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Organoid cryopreservation\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be prepared by yourself: 15 mL EP tube, cell cryopreservation tube, program cooling box, pipette gun.\u003cbr\u003e(2) Kit reagents: organoid subculture buffer G (4 ℃), organoid cryopreservation solution F.\u003cbr\u003e2. Organoid cryopreservation:\u003cbr\u003eOrganoids that are not in use temporarily should be frozen and stored in a low temperature environment.\u003cbr\u003e(1) Aspirate the medium, add an equal volume of organoid subculture buffer G to each well, gently blow the matrigel with a pipette gun, collect it in a 15 mLEP tube, transfer it to an EP tube every 6-8 wells, and stand at 4 ℃ for 10-15 minutes. Centrifuge at 300 g for 5 minutes to discard the supernatant, add 2 mL of organoid cryopreservation solution F every three wells, mix well by gently pipetting, and transfer to cell cryopreservation tubes, each tube containing 1 mL.\u003cbr\u003e(2) Make the mark information, put it in the program cooling box, move it to the-80 ℃ refrigerator, and put it in the liquid nitrogen tank for storage after 48 hours. Or put it in a 4 ℃ refrigerator for 40 minutes, put it in a-20 ℃ refrigerator for 2 hours, move it to a-80 ℃ refrigerator, and after 48 hours, put it in a liquid nitrogen tank for storage.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e5. Organoid resuscitation culture\u003c\/strong\u003e\u003cbr\u003e1. Experimental materials:\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e(1) Materials to be brought by yourself: Matrigel (melted in a 4 ℃ refrigerator 24 hours in advance), 15 mL EP tube, water bath, pipette gun, 24-well cell culture plate, ice box.\u003cbr\u003e(2) Kit reagents: organoid subculture buffer G (4 ℃), bovine normal intestinal organoid culture medium A.\u003cbr\u003e2. Organoid resuscitation culture:\u003cbr\u003e(1) Take out the frozen organoids from the low temperature environment and quickly put them in a 37 ℃ water bath to thaw. During the thawing process of the water bath, the cryopreservation tube should be gently shaken to ensure that the cryopreservation solution is completely melted in a short time. The thawed organoids were quickly transferred to 15 mLEP tubes, gently pipetted 6-8 times with a pipette gun, and centrifuged at 300 g for 5 minutes to discard the supernatant; Add an appropriate amount of passage buffer G to resuspend, transfer it into a 1.5 mL EP tube at 300 g and centrifuge for 5 minutes, and discard the supernatant.\u003cbr\u003e(2) Add 100 uL of Matrigel to each cryopreservation tube for resuspension, dispense 24-well cell culture plates according to 25 uL-30 uL\/well, and maintain the Matrigel at 0-4 ℃ throughout the process. Place the cell culture plate in a 37 ℃ incubator for 10-15 minutes. After the matrigel coagulates, add 500-750 uL of bovine normal intestinal organoid medium A (preheated at 37 ℃ in advance) to each well for culture.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003eKit composition:\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBovine normal intestinal organoid medium A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003ePrimary culture buffer B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBovine normal intestinal primary tissue digestive juice C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid passage digestive juice D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eTissue preservation solution E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eOrganoid Cryopreservation Solution F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003eOrganoid Subculture Buffer G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Bovine normal intestinal organoid culture medium A can be stored at 4 ℃ for 3 months. It is stored at 4 ℃ after receiving the goods. It is recommended to use it within 1 month. It is recommended to store it at-20 ℃ for a long time to avoid repeated freezing and thawing more than 2 times\u003cstrong\u003e\u003cstrong\u003e。\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e2. Tissue preservation solution E and bovine normal intestinal primary tissue digestive juice C contain nutrients to maintain cell activity. In order to maintain the activity of reagent nutrients, it is not recommended to store them at-20 ℃ for a long time to avoid repeated freezing and thawing more than twice.\u003cbr\u003e3. Under ambient conditions of 2-8 °C, matrigel was thawed overnight. When using Matrigel, keep it in an ice box to prevent premature setting. Matrigel formed a gel within 20 min at 37 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 4 ℃, valid for 3 months; Store at-20 ℃, shelf life is 1 year, see label for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41683109314635,"sku":null,"price":1032.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_b63b7f59-0d47-46ea-873d-972ca16bcc61.png?v=1770720294"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/collections\/absin_empty_f50294b5-722f-41eb-a0f1-8e730e2bfb6b.png?v=1766559061","url":"https:\/\/www.antbioinc.com\/collections\/organoid-kit.oembed?page=7","provider":"AntBio","version":"1.0","type":"link"}