{"title":"Organoid Culture Medium","description":null,"products":[{"product_id":"endometrial-ancer-like-organ-culture-medium","title":"Human Endometrial carcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human endometrial carcinoma organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human endometrial cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human endometrial cancer primary tissue digestive fluid for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of human endometrial carcinoma organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, human endometrial carcinoma organoid tissue primary culture buffer was added, and resuspended and centrifuged. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human endometrial cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human endometrial carcinoma organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add human endometrial cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human endometrial cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid medium for human endometrial carcinoma. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human endometrial carcinoma organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Human endometrial carcinoma organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human endometrial cancer organoid subculture buffer for resuspension and transfer. 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid medium for human endometrial carcinoma. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":41683108692043,"sku":"abs9637-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":41683108724811,"sku":"abs9637-500mL","price":1587.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_21126091-91b9-4dfe-8131-3e1874a2df14.jpg?v=1789747352"},{"product_id":"human-gastric-organoid-culture-medium","title":"Human Gastric Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human normal gastric organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human normal gastric organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary gastric tissue for tissue use #abs9783 digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of human normal gastric organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human normal gastric organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human normal gastric organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human normal gastric organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add human normal gastric organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human normal gastric organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Human normal gastric organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human normal gastric organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Human normal gastric organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human normal gastric organoid subculture buffer for resuspension and transfer. 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Human normal gastric organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43214373617739,"sku":"abs9736-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43214373650507,"sku":"abs9736-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"breast-cancer-organoid-culture-medium-abs9526","title":"Human Breast Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human breast cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human breast cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human breast cancer primary tissue digestive fluid for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human breast cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human breast cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human breast cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human breast cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human breast cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human breast cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human breast cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human breast cancer organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human breast cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human breast cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human breast cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe human breast cancer organoid basic culture medium can be applied to the conventional culture and passage of human breast cancer-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43214371618891,"sku":"abs9526-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43214371651659,"sku":"abs9526-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"organoid-cryopreservation-medium-abs9519","title":"Organoid Cryopreservation Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Add pre-cooled (2-8℃) organoid cryopreservation solution to the recovered organoids (or cells), blow and mix well, and then quickly transfer to the cell cryopreservation tube (the volume of cell suspension in the cryopreservation tube should be greater than 0.5ml). Note: It is recommended to cryopreserve 1x10 per 1mL of cryopreservation solution.\u003csup\u003e3\u003c\/sup\u003e-1x10\u003csup\u003e7\u003c\/sup\u003eAn organoid with a number of cells or a corresponding number of cells.\u003cbr\u003e2. Place the cryopreservation tube into the cell cryopreservation cooling box (the cooling box must be equilibrated to room temperature or 4℃ in advance), and then immediately place the cooling box into a -80℃ ultra-low temperature refrigerator; The cryopreservation tubes can also be subjected to artificial gradient cooling treatment, such as standing at 4℃ for 10 minutes, storing at -20℃ for 1 hour, and storing at -80℃ overnight.\u003cbr\u003e3. The next day or 12 hours later, transfer the cryopreservation tubes to liquid nitrogen for long-term storage under low temperature conditions (not higher than -70℃, dry ice or the original cooling box is recommended).\u003cbr\u003e\u003cbr\u003eIf the organoid (or cell) is resuscitated, the following steps can be performed:\u003cbr\u003e1. Transfer 10 mL of organoid culture buffer B to a 15 mL centrifuge tube.\u003cbr\u003e2. Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in a 37℃ water bath to thaw.\u003cbr\u003e3. During the water bath thawing process, the freezing tube should be gently shaken to ensure that the frozen solution is completely thawed within 1-2 minutes.\u003cbr\u003e4. Quickly transfer the lysed organoid cells to a 15 mL centrifuge tube, gently blow 6-8 times with a pipette, centrifuge 300 g for 5 minutes, then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid buffer B, resuspend, transfer to a 1.5 ml centrifuge tube, 300 g, and centrifuge for 5 min.\u003cbr\u003e5. Resuspend the matrix gel. Place 25 μL of matrix gel per well in a 24-well cell culture plate, place it in the incubator for 10-15 minutes to form gel, and add 500 μL of organoid medium A.\u003cbr\u003e\u003cbr\u003eNote: Organoid cryopreservation solution (abs9519) can be used with organoid culture medium.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganoid Cryopreservation mediums can be used for the long-term Cryopreservation of organoids and cell lines derived from various mammals (such as humans and mice).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20°C, shelf life 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"50mL","offer_id":43224141463627,"sku":"abs9519-50mL","price":111.0,"currency_code":"USD","in_stock":true},{"title":"100mL","offer_id":43224141496395,"sku":"abs9519-100mL","price":190.0,"currency_code":"USD","in_stock":true}]},{"product_id":"organoid-basal-solution-abs9730","title":"Organoid Subculture Buffer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eOrganoid subculture buffer can clean organoids gently and quickly. This buffer is widely used for organoid subculture cleaning of solid tumors (such as colon cancer, lung cancer, breast cancer, endometrial cancer, etc.). Passionate digestion terminated. It has protective and bacteriostatic effects on organoids.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore at -20℃ for 1 year.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"250mL","offer_id":43224141561931,"sku":"abs9730-250mL","price":29.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141594699,"sku":"abs9730-500mL","price":51.0,"currency_code":"USD","in_stock":true}]},{"product_id":"organoid-basal-solution-abs9731","title":"Organoid Primary Culture Buffer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTissue primary culture buffer can gently and quickly clean blood and other impurities from tumor tissue samples. This buffer is widely applicable to solid tumors (such as intestinal cancer, lung cancer, breast cancer, endometrial cancer, etc.) and normal tissue specimens.\u003cbr\u003eTissue primary buffer can be used for tissue cleaning, primary digestion termination, etc. during primary culture, and plays a protective and bacteriostatic role in tissues.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore at -20℃ for 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"250mL","offer_id":43224141660235,"sku":"abs9731-250mL","price":32.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141693003,"sku":"abs9731-500mL","price":57.0,"currency_code":"USD","in_stock":true}]},{"product_id":"organoid-digestion-solution-abs9520","title":"Organoid Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Pipette off the culture medium, add 1 ml of 4℃ organoid buffer B to each well and leave for 2 min. Pipette and gently blow the matrix gel, collect it in a 15 ml centrifuge tube, and let it stand at 4℃ for 10 min. (One set per 6-8 wells).\u003cbr\u003e2. Centrifuge for 5 min to discard the supernatant, add an appropriate amount of organoid passage digestion solution to digest for 2-3 min, add 5 times the volume of organoid buffer to terminate the digestion, centrifuge for 5 min to discard the mixture, add organoid buffer B, resuspend and transfer into a 5 ml centrifuge tube, centrifuge 300 g for 5 min to discard the liquid for step 3.\u003cstrong\u003eNote:\u003c\/strong\u003e① The digestion process must be carefully monitored during this operation to avoid excessive digestion. During digestion, a pipette was used to blow and mix to aid digestion. A small amount of digestion suspension can also be taken in real time to observe the digestion under a microscope. When a large number of single cells or cell clusters with a diameter of less than 50 um are observed, the digestion can be considered complete. ② Add at least 5 times the volume of organoid buffer to the suspension after confirming the completion of digestion for dilution to terminate the digestion.\u003cbr\u003e3. The organoid precipitate obtained in the previous step can be used for subsequent experimental operations such as organoid culture and cryopreservation.\u003cbr\u003e\u003cbr\u003eNote: Organoid passage digestion solution can be used in conjunction with organoid culture medium.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganoid Digestion Solution can be applied to the routine passage of organoids of various mammalian (such as human, mouse) origin. It can separate the organoids from the matrix gel and gently digest them into small cell clusters or single cells, while maintaining their growth viability after passage.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20°C, shelf life 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224141758539,"sku":"abs9520-100mL","price":160.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141791307,"sku":"abs9520-500mL","price":803.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-tumor-tissue-digestion-solution-abs9644","title":"Mouse Intestinal Tumor Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3 mm.\u003csup\u003e3\u003c\/sup\u003eTissue blocks.\u003cbr\u003e2. Add an appropriate volume of tissue digestive fluid (the digestive fluid volume should be 5-10 times the original tissue volume) according to the size of the original tissue block; Tissue digestion was performed in a 4℃ constant temperature incubator or constant temperature shaker, with an overall digestion time of 15-20 min.\u003cbr\u003e\u003cstrong\u003eNote:\u003c\/strong\u003eThe digestion process must be carefully monitored because excessive digestion may affect cell viability and significantly reduce the organoid success rate. During the digestion process, the digestion suspension can be microscopically examined. Digestion can be considered complete when a large number of individual cells or cell clusters smaller than 70 μm are observed under the microscope.\u003cbr\u003e3. Add 3 times organoid culture buffer to the tissue suspension after confirming the completion of digestion to terminate digestion.\u003cbr\u003e4. The digestion suspension obtained in the above step can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300 g centrifugation for 3 min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eMouse small intestine-specific primary tissue digestion solution can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is widely applicable to the digestive dissociation of mouse intestine in vitro primary culture.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224141856843,"sku":"abs9644-100mL","price":160.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141889611,"sku":"abs9644-500mL","price":714.0,"currency_code":"USD","in_stock":true}]},{"product_id":"intestines-tissue-digestion-solution-abs9749","title":"Intestines Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e(1) It is recommended to store the tissue after sampling at 2-8℃, quickly transfer it to a clean laboratory for tissue processing and stem cell separation, take photos and register the information.\u003cbr\u003e(2) Prepare several petri dishes and add 4℃ pre-cooled primary buffer (or PBS) for later use.\u003cbr\u003e(3) Disinfect the sample tube, place the tissue in a petri dish, wash it three times with primary buffer (or PBS), remove impurities, and cut the tissue into a volume of about 1~3mm with ophthalmic scissors or scalpels.\u003csup\u003e3\u003c\/sup\u003eTissue blocks. (It is recommended to add 1.5ml EP tube to shear the tissue)\u003cbr\u003e(4) Add 5ml of primary intestinal tissue digestion solution to the shredded tissue block and shake for 15-25min at 4℃ for digestion (observe the tissue digestion at any time during the digestion process).\u003cbr\u003eNote: If the amount of tissue is too small or the biopsy sample is digested with 1ml of primary tissue digest in a 1.5ml EP tube.\u003cdiv\u003e(5) Take a small amount of digestive fluid and observe it under a microscope. After observing a large number of cell clusters or individual cells below 70 μm under the microscope (Figure 1-2), add 3 times the volume of primary buffer (or PBS) to stop the digestion.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20260731\/783d6c0afb6646a0936276b90ea663d4.jpg\" alt=\"\" width=\"399\" height=\"200\"\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eHuman normal intestinal primary tissue digestive fluid can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is suitable for digestive dissociation of human normal intestine in vitro primary culture.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe digestive juice of normal intestinal tissue contains nutrients that maintain cell activity and a mixture of various biological enzymes, avoiding repeated freezing and thawing more than twice.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224141955147,"sku":"abs9749-100mL","price":214.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141987915,"sku":"abs9749-500mL","price":894.0,"currency_code":"USD","in_stock":true}]},{"product_id":"gaster-tissue-digestion-solution-abs9783","title":"Gaster Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3mm.\u003csup\u003e3\u003c\/sup\u003eTissue blocks.\u003cbr\u003e2. Add an appropriate volume of tissue digestive fluid (the volume of digestive fluid should be 5-10 times the volume of the original tissue) according to the size of the original tissue block; Tissue digestion is carried out in a 4 DEG C constant temperature incubator or a constant temperature shaker, and the digestion time is 15-20 minutes.\u003cstrong\u003eNote:\u003c\/strong\u003eThe digestion process must be carefully monitored because excessive digestion may affect cell viability and significantly reduce the success rate of organoid construction. During the digestion process, the digestion suspension can be microscopically examined. After a large number of single cells or cell clusters below 70um are observed under the microscope, the digestion can be considered complete.\u003cbr\u003e3. Add 3 times primary tissue culture buffer to the tissue suspension after confirming the completion of digestion to terminate the digestion.\u003cbr\u003e4. The digestion suspension obtained in the above steps can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300g for 3 minutes).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGaster Tissue Digestion Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman normal gastric primary tissue digestive fluid can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is suitable for digestion and dissociation of normal gastrointestinal tissue specimens in vitro primary culture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142053451,"sku":"abs9783-100mL","price":214.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224142086219,"sku":"abs9783-500mL","price":894.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-gaster-tissue-digestion-solution-abs9790","title":"Murine Gaster Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3mm.\u003csup\u003e3\u003c\/sup\u003eTissue blocks.\u003cbr\u003e2. Add an appropriate volume of tissue digestive fluid (the volume of digestive fluid should be 5-10 times the volume of the original tissue) according to the size of the original tissue block; Tissue digestion is carried out in a 4 DEG C constant temperature incubator or a constant temperature shaker, and the digestion time is 15-20 minutes.\u003cbr\u003eNote: The digestion process must be carefully monitored, as excessive digestion may affect cell viability and significantly reduce the success rate of organoid construction. During the digestion process, the digestion suspension can be microscopically examined. Digestion can be considered complete after a large number of individual cells or cell clusters smaller than 70 μm are observed under the microscope.\u003cbr\u003e3. Add 3 times primary tissue culture buffer to the tissue suspension after confirming the completion of digestion to terminate the digestion.\u003cbr\u003e4. The digestion suspension obtained in the above step can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300g for 3 minutes).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMurine Gaster Tissue Digestion Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe digestive fluid of primary tissue of normal mouse stomach can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestion solution is suitable for digestion and dissociation of rat normal gastrointestinal tissue specimens in vitro primary culture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142217291,"sku":"abs9790-100mL","price":214.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224142250059,"sku":"abs9790-500mL","price":894.0,"currency_code":"USD","in_stock":true}]},{"product_id":"organoid-recovery-solution-abs9920","title":"Organoid Retrieval Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Organoid collection: Under the condition of retaining the original culture medium in the well plate (or removing the culture medium and adding an equal volume of basal culture medium), gently scrape off the matrix gel and organoid mixture with the tip of the pipette tip (do not blow vigorously, just collect), transfer to 1.5ml, and then let stand for 2-10min (or centrifuge 100g at low speed for 3min).\u003cbr\u003e2. Add recovery solution: Retain the matrix gel and organoid mixture. After discarding the supernatant, add 10 times the volume of organoid recovery solution according to the precipitation amount (recommended precipitation amount of 50 μL\/tube). Shake gently to allow the precipitate to fully contact the recovery solution.\u003cbr\u003e3. Incubation: Incubate on ice for 30 minutes. During the incubation process, shake gently 3-4 times every 5 minutes.\u003cbr\u003e4. Centrifuge: Centrifuge 250g for 3 minutes, discard the supernatant.\u003cbr\u003e5. Washing: Add pre-cooled epithelial organoid-specific basic medium to wash the organoids once, and centrifuge to discard the supernatant to obtain organoid precipitate.  \u003cbr\u003e6. The recovered organoids can be used for downstream experiments and biochemical analysis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is a ready-to-use solution for recovering intact organoids from matrix glue. The organoid recovery solution can rapidly dissolve the matrix gum in an environment of 2-8°C without causing damage to the shape or structure of the organoid throughout the process. Organoids obtained from organoid recovery solution can be used for organoid passage, cryopreservation, and other related experimental studies, such as RNA and protein extraction, organoid section staining, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2-8 °C, protected from light, shelf life 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142413899,"sku":"abs9920-100mL","price":238.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-skin-digestion-solution-abs9931","title":"Human Skin Organoid Dissociation Reagent","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Own reagents required: \u003c\/strong\u003e\u003cbr\u003e1.  horizontal rotor centrifuge ( Can be reduced to 4℃)\u003cbr\u003e2.   biological safety cabinet \/ ultra-clean workbench \u003cbr\u003e3.  CO2 incubator (5%CO2 ， 37℃)\u003cbr\u003e4.   Low temperature operating table \u003cbr\u003e5.   refrigerator ( 2-8°C ） \u003cbr\u003e6.   water bath \/ metal bath \u003cbr\u003e7.   pipette \u003cbr\u003e8.  Cytometer \u003cbr\u003e9.  inverted microscope \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e Reagent dispensing: \u003c\/strong\u003e\u003cbr\u003e Tissue digestive juice preparation \u003cbr\u003e1. Skin tissue digestive juice before use Ⅰ From -20 ℃ Taking it out of the environment, placing it at room temperature to melt it, turning the bottle upside down several times after it is completely dissolved to fully mix the liquid; \u003cbr\u003e2.5 mL skin tissue digestive fluid Ⅰ Add basic medium ( DMEM ) Set the volume to 12.5 mL After configuration, record the preparation date on the label. 2- 8℃ Store in the refrigerator away from light; \u003cbr\u003e\u003cstrong\u003e Note: Skin tissue digestive fluid Ⅰ After preparation, it is recommended to... 1 Used within a month; \u003c\/strong\u003e\u003cbr\u003e3. Formulation contains 5%FBS 、 1% penicillin - streptomycin DMEM The neutralization solution was used to stop the digestion. \u003cbr\u003e\u003cstrong\u003e Note: The centrifuge tubes, gun tips, etc. used in this experiment must be washed in advance with a special washing solution. \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e Preparation before operation: \u003cbr\u003e\u003c\/strong\u003e1. The centrifuge temperature is set to  4℃ Pre-cooling; \u003cbr\u003e2. The sampling gun tip was placed in -20 ℃ Precool and remove before sample addition; \u003cbr\u003e3.24  The orifice plate is placed in  37℃ Preheating the constant temperature incubator;  \u003cbr\u003e4. The matrix glue is placed on top of ice or  4℃ refrigerator 2-3 h melt; \u003cbr\u003e\u003cstrong\u003e Note: Keep the matrix glue in place during use. 4℃ The following (it is recommended to store on ice) will cause the matrix glue to solidify and become unusable due to increased temperature.  \u003c\/strong\u003e\u003cbr\u003e5. The centrifuge tube is washed by the washing liquid and then placed on ice for precooling;  \u003cbr\u003e6. The dispensed human epidermal organoid medium was equilibrated to room temperature; \u003cbr\u003e\u003cstrong\u003e Note: If the matrix gel needs to be diluted, the dilution medium can be placed in... 4℃ Pre-cooling; \u003c\/strong\u003e\u003cbr\u003e7.DPBS  Place on ice to precool. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e Operating instructions: \u003cbr\u003e\u003cbr\u003e Tissue Digestion: \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e Attention! During the organoid procedure, all centrifuge tubes and pipette tips...   Consumables that come into contact with organoids, such as disposable straws and cell screens, must be washed with a special washing solution before operation to reduce cell loss. \u003c\/strong\u003e\u003cbr\u003e1. Flatten the annular foreskin tissue of the surgical sample on 10 cm In a petri dish, contain 1% penicillin - streptomycin DPBS Soaking in the solution, repeatedly rinsing to remove residual blood; \u003cbr\u003e2. Remove subcutaneous structures of tissues, such as fat, blood vessels, etc., and rinse several times; \u003cbr\u003e3. Cut the tissue evenly into 0.5-1.5 cm Square blocks of different sizes on the left and right sides, rinse again until the rinsing liquid is clear; \u003cbr\u003e4. Place the washed small pieces of tissue in 10 mL Freshly prepared skin tissue digestive fluid Ⅰ In the middle, with the epidermis layer facing down, placed in 4℃ Digest overnight in the refrigerator; \u003cbr\u003e\u003cstrong\u003e Note: The recommended digestion time is 12-14 h ； \u003c\/strong\u003e\u003cbr\u003e5. After overnight digestion, the epidermis and dermis of the foreskin tissue were separated by gentle pulling with sterile forceps   , collecting epidermal skin pieces; \u003cbr\u003e6. Cut the epidermis into a paste with sterile surgical scissors and place it in a petri dish; \u003cbr\u003e7. join 3 mL skin tissue digestive fluid Ⅱ , at 37℃ Digestion in the incubator 10 minutes; \u003cbr\u003e8. Placing the culture dish under a microscope to observe the digestion; \u003cbr\u003e\u003cstrong\u003e Note: You can gently tap the dish wall. A large number of floating and free cell bright spots can be seen under the microscope, which indicates that the digestion process is complete. Otherwise, the digestion time can be appropriately extended. \u003c\/strong\u003e\u003cbr\u003e9. After digestion is complete, add 3 mL Contains 5%FBS 、 1% penicillin - streptomycin DMEM The neutralization solution terminates digestion; \u003cbr\u003e10. Repeatedly and gently blowing to disperse the cell mass; \u003cbr\u003e11. Moisturizing washing liquid 40 μm  Cell screens and cell collection 50 mL a centrifuge tube; \u003cbr\u003e12.40 μm Cell sieve filtration, and the filtrate is collected until washed. 50 mL in a centrifuge tube; \u003cbr\u003e13.4℃ ， 400 g Centrifugation 5 min ； \u003cbr\u003e14. Use DPBS resuspending the cell pellet; \u003cbr\u003e15.4℃ ， 400 g Centrifugation 5 min 。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e Organoid Culture: \u003cbr\u003e Attention! During the organoid procedure, all centrifuge tubes and pipette tips...   Consumables such as disposable straws that come into contact with organoids must be washed with a special washing solution before operation to reduce cell loss. \u003cbr\u003e\u003c\/strong\u003e1. Add an appropriate amount of pre-cooled matrix gum to the precipitate and mix gently.  10-15  Next;  \u003cbr\u003e2. Take an appropriate volume of the mixed solution and observe the density under a microscope; \u003cbr\u003e3. Add an appropriate amount of matrix glue to adjust the density, and blow and mix well.  10-15  Next;  \u003cbr\u003e\u003cstrong\u003e Note: This step should be done as quickly as possible to avoid solidification of the matrix glue due to increased temperature; Do not generate air bubbles when blowing and beating; Please adjust the density appropriately according to the cell size; \u003c\/strong\u003e\u003cbr\u003e4.50 μL \/ The matrix glue and organoid suspension were added dropwise to the hole.  24  The center of the orifice plate is hemispherical; \u003cbr\u003e5.37℃ 、 5% CO2 Incubation in incubator  20 min Avoid shaking the culture plate during the process;  \u003cbr\u003e6. Add per well after incubation 500 μL human epidermal organoid medium;  \u003cbr\u003e7.37℃ 、 5% CO2 Continue cultivating in the incubator, each  2-3  Change the fresh medium once a day.  \u003cbr\u003e\u003cstrong\u003e Note: Observe daily after inoculation, and the entire operation should comply with aseptic operating procedures. \u003cbr\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThis kit is suitable for effective dissociation of primary keratinocytes, thereby rapidly obtaining cell masses or single-cell suspensions, and the obtained cells can be used for subsequent organoid culture.\u003cbr\u003e\u003cstrong\u003eProduct Composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 57.1428%; height: 67px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 33px;\"\u003e\n\u003ctd style=\"width: 29.0449%; text-align: center; height: 33px;\"\u003ecomponents\u003c\/td\u003e\n\u003ctd style=\"width: 23.8%; text-align: center; height: 33px;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 29.0449%; height: 20px;\"\u003eSkin tissue digestive fluid Ⅰ\u003c\/td\u003e\n\u003ctd style=\"width: 23.8%; height: 20px;\"\u003e5mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 29.0449%; height: 14px;\"\u003eSkin tissue digestive fluid Ⅱ\u003c\/td\u003e\n\u003ctd style=\"width: 23.8%; height: 14px;\"\u003e5mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1.\u003cstrong\u003eThis product contains active agents. Please store the reagents strictly according to the kit instructions. Repeated freezing and thawing or improper storage will affect the experimental results.\u003c\/strong\u003e\u003cbr\u003e2. This product is only suitable for scientific research and cannot be used in diagnostic or therapeutic fields.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2-8℃ in a dark place. Shelf life is 3 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":43224142512203,"sku":"abs9931-1kit","price":233.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hepatocarcinoma-organoid-culture-medium-abs9527","title":"Human Hepatocarcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human liver cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human liver cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human liver cancer tissue for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human hepatocellular carcinoma organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human hepatocellular carcinoma organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human liver cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human liver cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add human liver cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human liver cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Organoid medium for human hepatocellular carcinoma. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human hepatocellular carcinoma organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human liver cancer organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human liver cancer organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Organoid medium for human hepatocellular carcinoma. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003esolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe human liver cancer organoid basic culture medium can be applied to the routine culture and passage of human liver cancer-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142610507,"sku":"abs9527-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224142643275,"sku":"abs9527-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"intestinal-cancer-organoid-culture-medium-abs9523","title":"Human Intestinal Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human colon cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human colorectal cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human colorectal cancer primary tissue digestive fluid for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human colon cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human intestinal cancer organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human intestinal cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human colorectal cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add human colorectal cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human colorectal cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Organoid medium for human intestinal cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human intestinal cancer organoid subculture buffer and resuspending again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human intestinal cancer organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human colorectal cancer organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Organoid medium for human intestinal cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe human intestinal cancer organoid basic culture medium can be applied to the conventional culture and passage of human intestinal cancer-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142741579,"sku":"abs9523-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224142774347,"sku":"abs9523-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"gastric-cancer-organoid-culture-medium-abs9524","title":"Human Gastric Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human gastric cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human gastric cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissues of human gastric cancer for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e ）   digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  7μm  After the following cell clusters, digestion was terminated by adding three times the volume of human gastric cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human gastric cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e ）   Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human gastric cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human gastric cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e ）   digestion 2-3min Add human gastric cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human gastric cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human gastric cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human gastric cancer organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human gastric cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human gastric cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human gastric cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe human gastric cancer organoid basic culture medium can be applied to the conventional culture and passage of human gastric cancer-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143364171,"sku":"abs9524-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143396939,"sku":"abs9524-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"lung-cancer-organoid-culture-medium-abs9525","title":"Human Lung Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human lung cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human lung cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human lung cancer tissue for tissue use ( abs9522 ）   digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human lung cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human lung cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 ）   Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human lung cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human lung cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 ）   digestion 2-3min Add human lung cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human lung cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human lung cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human lung cancer organoid subculture buffer and resuspending again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human lung cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human lung cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Organoid medium for human lung cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe human lung cancer organoid basic culture medium can be applied to the routine culture and passage of human lung cancer-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143462475,"sku":"abs9525-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143495243,"sku":"abs9525-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"pancreatic-cancer-organoid-culture-medium-abs9528","title":"Human Pancreatic Cancer Organoid CultureMedium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human pancreatic cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human pancreatic cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human pancreatic cancer primary tissue digestive fluid for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human pancreatic cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human pancreatic cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human pancreatic cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human pancreatic cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human pancreatic cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human pancreatic cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human pancreatic cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human pancreatic cancer organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Human pancreatic cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human pancreatic cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human pancreatic cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eHuman pancreatic cancer organoid basal culture medium is a culture medium developed to promote the in vitro formation and expansion of human pancreatic cancer organoids. It is a sterile liquid mixing system containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, growth factors, trace minerals, etc. The unique formulation of the culture medium can provide an optimal in vitro culture environment for human pancreatic cancer organoids and selectively promote the growth of human pancreatic cancer organoids in vitro.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143593547,"sku":"abs9528-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143626315,"sku":"abs9528-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-liver-organoid-culture-medium-abs9529","title":"Human Liver Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human normal liver organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human normal liver organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human liver primary tissue for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human normal liver organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human normal liver organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human normal liver organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human normal liver organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human normal liver organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human normal liver organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Human normal liver organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human normal liver organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Human normal liver organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human normal liver organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Human normal liver organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe human normal liver organoid basic culture medium can be applied to the conventional culture and passage of human normal liver-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143691851,"sku":"abs9529-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143724619,"sku":"abs9529-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-intestinal-organoid-culture-medium-abs9530","title":"Human Intestinal Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation:  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human normal intestinal organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human normal intestinal organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid for tissues ( abs9749 Digestion, 4℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human normal intestinal organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human normal intestinal organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human normal intestinal organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human normal intestinal organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human normal intestinal organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human normal intestinal organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Human normal intestinal organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human normal intestinal organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human normal intestinal organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human normal intestinal organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Human normal intestinal organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe human normal intestine organoid basic culture medium can be applied to the routine culture and passage of the human normal intestine-derived organoid, and can maintain its growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143790155,"sku":"abs9530-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143822923,"sku":"abs9530-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-lung-organoid-culture-medium-abs9531","title":"Human Lung Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human normal lung organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human normal lung organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human lung primary tissue for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human normal lung organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human normal lung organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human normal lung organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human normal lung organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add human normal lung organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human normal lung organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Culture medium for normal human lung organoids. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human normal lung organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Human normal lung organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human normal lung organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Culture medium for normal human lung organoids. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe human normal lung organoid basic culture medium can be applied to the conventional culture and passage of human normal lung-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224143921227,"sku":"abs9531-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224143953995,"sku":"abs9531-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-pancreatic-organoid-culture-medium-abs9532","title":"Human Pancreatic Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human normal pancreatic organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human normal pancreatic organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary pancreatic tissue for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human normal pancreatic organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100 position m The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human normal pancreatic organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human normal pancreatic organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human normal pancreatic organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human normal pancreatic organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human normal pancreatic organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Human normal pancreatic organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human normal pancreatic organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human normal pancreatic organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human normal pancreatic organoid subculture buffer for resuspension and transfer. 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Human normal pancreatic organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe human normal pancreas organoid basic culture medium can be applied to the conventional culture and passage of human normal pancreas-derived organoids, can maintain their growth vitality after passage, and does not need to add any other protein factors during the organoid culture process.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144019531,"sku":"abs9532-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144052299,"sku":"abs9532-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-intestinal-cancer-organoid-culture-medium-abs9533","title":"Mouse Intestinal Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse colon cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with primary culture buffer for mouse colorectal cancer organoids, and then the tissue is cut into a body using ophthalmic scissors or a scalpel. \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of mouse intestinal cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm  The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse intestinal cancer organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e ）   Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse intestinal cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse colorectal cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add mouse colorectal cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse colorectal cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for mouse intestinal carcinoma. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse intestinal carcinoma organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse intestinal cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse intestinal cancer organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for mouse intestinal carcinoma. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe basic culture medium for mouse intestinal cancer organoids can be applied to the conventional culture and passage of mouse intestinal cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144117835,"sku":"abs9533-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144150603,"sku":"abs9533-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-gastric-cancer-organoid-culture-medium-abs9534","title":"Mouse Gastric Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse gastric cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, the tissue was placed in a petri dish, washed three times with primary culture buffer for mouse gastric cancer organoids, and then the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse gastric cancer for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of mouse gastric cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse gastric cancer organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse gastric cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse gastric cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add mouse gastric cancer organoid subculture buffer to terminate digestion and centrifuge. 5min The mixture was discarded, and an appropriate amount of mouse gastric cancer organoid subculture buffer was added for resuspension and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid culture medium for mouse gastric cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse gastric cancer organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse gastric cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse gastric cancer organoid subculture buffer for resuspension and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Organoid culture medium for mouse gastric cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe basic culture medium for mouse gastric cancer organoids can be applied to the conventional culture and passage of mouse gastric cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144216139,"sku":"abs9534-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144248907,"sku":"abs9534-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-lung-cancer-organoid-culture-medium-abs9535","title":"Mouse Lung Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse lung cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, the tissue was placed in a petri dish, washed three times with mouse lung cancer organoid tissue primary culture buffer, and then the tissue was cut into approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse lung cancer for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, three times the volume of mouse lung cancer organoid tissue primary culture buffer was added to stop digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse lung cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min After gelatinization, mouse lung cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse lung cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add mouse lung cancer organoid subculture buffer to terminate digestion and centrifuge. 5min The mixture was discarded, and an appropriate amount of mouse lung cancer organoid subculture buffer was added for resuspension and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for mouse lung cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse lung cancer organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse lung cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse lung cancer organoid subculture buffer for resuspension and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for mouse lung cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe basic culture medium for mouse lung cancer organoids can be applied to the routine culture and passage of mouse lung cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144314443,"sku":"abs9535-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144347211,"sku":"abs9535-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-breast-cancer-organoid-culture-medium-abs9536","title":"Mouse Breast Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse breast cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, the tissue was placed in a petri dish, washed three times with primary culture buffer for mouse breast cancer organoids, and then the tissue was cut into approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse breast cancer for tissue use \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of mouse breast cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse breast cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse breast cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse breast cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add mouse breast cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse breast cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for breast cancer in mice. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse breast cancer organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse breast cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse breast cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for breast cancer in mice. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe basic culture medium for mouse breast cancer organoids can be applied to the routine culture and passage of mouse breast cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144445515,"sku":"abs9536-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144478283,"sku":"abs9536-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-hepatocarcinoma-organoid-culture-medium-abs9537","title":"Mouse Hepatocarcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer for mouse hepatocellular carcinoma organoid tissue #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, the tissue was placed in a petri dish, washed three times with primary culture buffer for mouse liver cancer organoids, and then the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse liver cancer for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, three times the volume of mouse hepatocellular carcinoma organoid tissue primary culture buffer was added to stop the digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse liver cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse liver cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse liver cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add mouse liver cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse liver cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid medium for mouse hepatocellular carcinoma. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse hepatocellular carcinoma organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Mouse liver cancer organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse hepatocellular carcinoma organoid subculture buffer to resuspend and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid medium for mouse hepatocellular carcinoma. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe basic culture medium for mouse liver cancer organoids can be applied to the routine culture and passage of mouse liver cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144543819,"sku":"abs9537-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144576587,"sku":"abs9537-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-pancreatic-cancer-organoid-culture-medium-abs9538","title":"Mouse Pancreatic Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse pancreatic cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, the tissue was placed in a petri dish, washed three times with mouse pancreatic cancer organoid tissue primary culture buffer, and then the tissue was cut into approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse pancreatic cancer for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of mouse pancreatic cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse pancreatic cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7  6 After the step, observe the collected tissue volume and add 25  \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e  8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e  9 Place the prepared culture plate in... 37℃  10-15min Gelatinization was performed, and mouse pancreatic cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2  \u003c\/strong\u003e\u003cbr\u003e （ 1  1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e  2min 。 \u003cbr\u003e （ 2  15mL In the centrifuge tube, 4℃  10min 。 (each 6-8  \u003cbr\u003e （ 3 ） a    centrifuge 5min  1.5mL centrifuge tube, 300g  5min Discard the liquid for the first 4  \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add mouse pancreatic cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse pancreatic cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g  5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  24 In well cell culture plates, place in an incubator. 10-15min  500μL  Organoid medium for mouse pancreatic cancer. \u003cbr\u003e\u003cstrong\u003e3  \u003c\/strong\u003e\u003cbr\u003e （ 1  1-2mL 4℃ organoid subculture buffer placement 2min  \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml  4℃ standing 10min  6-8 (The holes are a group) \u003cbr\u003e  3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse pancreatic cancer organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5  \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e  1 ) take 10mL Mouse pancreatic cancer organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3   1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse pancreatic cancer organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003esolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basic culture medium for mouse pancreatic cancer organoids can be applied to the routine culture and passage of mouse pancreatic cancer-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144642123,"sku":"abs9538-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144674891,"sku":"abs9538-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-liver-organoid-culture-medium-abs9539","title":"Mouse Liver Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse normal liver organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with primary culture buffer for normal mouse liver organoids, the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal primary liver tissue of mice for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e ）   digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, three times the volume of mouse normal liver organoid tissue primary culture buffer was added to terminate the digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse normal liver organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e ）   Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and normal mouse liver organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of normal mouse liver organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e)  digestion 2-3min Add normal mouse liver organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of normal mouse liver organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Mouse normal liver organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal liver organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse normal liver organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse liver organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Mouse normal liver organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse liver organoid culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basic culture medium of mouse normal liver organoids can be applied to the conventional culture and passage of mouse normal liver-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144740427,"sku":"abs9539-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144773195,"sku":"abs9539-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-intestinal-organoid-culture-medium-abs9540","title":"Mouse Intestinal Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestinal tissue of mice after sampling was placed in a precooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and stem cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse normal small intestine organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with primary culture buffer for normal mouse small intestine organoids, the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Primary tissue digestive solution specifically for normal small intestine tissue in mice for tissue use ( \u003cstrong\u003eabs9644\u003c\/strong\u003e digestion), 4℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  The following cell clusters were followed by the addition of three times the volume of mouse normal small intestine organoid tissue primary culture buffer to terminate digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse normal small intestine organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Twice the tissue volume of matrix glue was resuspended from the plates. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse normal small intestine organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse normal small intestine organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add normal mouse small intestine organoid subculture buffer to terminate digestion and centrifuge. 5min The mixture was discarded, and an appropriate amount of mouse normal small intestine organoid subculture buffer was added for resuspension and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Mouse normal small intestine organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal small intestine organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse normal small intestine organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse small intestine organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Mouse normal small intestine organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse small intestine organoid culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basic culture medium of mouse normal small intestine organoids can be applied to the routine culture and passage of mouse normal small intestine-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224144838731,"sku":"abs9540-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224144871499,"sku":"abs9540-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-lung-organoid-culture-medium-abs9541","title":"Mouse Lung Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse normal lung organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with primary culture buffer for normal mouse lung organoids, the tissue was cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal mouse lung primary tissue for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, three times the volume of mouse normal lung organoid tissue primary culture buffer was added to terminate the digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse normal lung organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse normal lung organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse normal lung organoid subculture buffer, resuspend and transfer 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add normal mouse lung organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse normal lung organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Mouse normal lung organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal lung organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Mouse normal lung organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse lung organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Mouse normal lung organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse lung organoid culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basic culture medium of mouse normal lung organoids can be applied to the routine culture and passage of mouse normal lung-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224145657931,"sku":"abs9541-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224145690699,"sku":"abs9541-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-pancreatic-organoid-culture-medium-abs9542","title":"Mouse Pancreatic Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer for normal mouse pancreatic organoid tissue ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with primary culture buffer for normal mouse pancreatic organoid tissue, the tissue was cut into pieces with ophthalmic scissors or a scalpel to a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal primary pancreatic tissue in mice for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  The following cell clusters were followed by the addition of three times the volume of mouse normal pancreatic organoid tissue primary culture buffer to terminate digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse normal pancreatic organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse normal pancreatic organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of normal mouse pancreatic organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add normal mouse pancreatic organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of normal mouse pancreatic organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Mouse normal pancreatic organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal pancreatic organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Mouse normal pancreatic organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse pancreatic organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Mouse normal pancreatic organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse pancreatic organoid culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basic culture medium of mouse normal pancreas organoids can be applied to the conventional culture and passage of mouse normal pancreas-derived organoids, and can maintain their growth vitality after passage. During the organoid culture process, no other protein factors need to be added.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224145821771,"sku":"abs9542-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224145854539,"sku":"abs9542-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bovine-mammary-glands-organoid-culture-medium-abs9638","title":"Bovine Mammary Glands Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer for mammary organoid tissue in dairy cows #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with primary culture buffer for dairy cow mammary organoids, and then the tissue is cut into pieces with ophthalmic scissors or a scalpel to a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary mammary tissue of dairy cows for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was stopped by adding three times the volume of dairy cow mammary organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and the primary culture buffer of dairy cow mammary organoid tissue was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min After gelatinization, dairy cow mammary gland organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of dairy cow mammary organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add dairy cow mammary organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of dairy cow mammary organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Dairy cow mammary gland organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of dairy cow mammary gland organoid subculture buffer solution to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Dairy cow mammary organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of dairy cow mammary organoid subculture buffer for resuspension and transfer. 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Dairy cow mammary gland organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224145920075,"sku":"abs9638-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224145952843,"sku":"abs9638-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cervical-cancer-organoid-culture-medium-abs9639","title":"Human Cervical Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation:  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human cervical cancer organoid tissue primary culture buffer ( abs9731 ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human cervical cancer organoid tissue primary culture buffer, and then the tissue is cut into pieces with ophthalmic scissors or a scalpel to a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human cervical cancer tissue for tissue use ( abs9522 Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human cervical cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human cervical cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human cervical cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human cervical cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add human cervical cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human cervical cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human cervical cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human cervical cancer organoid subculture buffer and resuspending, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( abs9519 ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Human cervical cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human cervical cancer organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human cervical cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146018379,"sku":"abs9639-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146051147,"sku":"abs9639-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"nasopharyngeal-carcinoma-organoid-culture-medium-abs9640","title":"Human Nasopharyngeal Carcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human nasopharyngeal carcinoma organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human nasopharyngeal carcinoma organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human nasopharyngeal carcinoma primary tissue digestive fluid for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human nasopharyngeal carcinoma organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human nasopharyngeal carcinoma organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human nasopharyngeal carcinoma organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human nasopharyngeal carcinoma organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add human nasopharyngeal carcinoma organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human nasopharyngeal carcinoma organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human nasopharyngeal carcinoma. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human nasopharyngeal carcinoma organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human nasopharyngeal carcinoma organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human nasopharyngeal carcinoma organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human nasopharyngeal carcinoma. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146116683,"sku":"abs9640-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146149451,"sku":"abs9640-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"ovarian-cancer-organoid-culture-medium-abs9641","title":"Human Ovarian Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human ovarian cancer organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human ovarian cancer organoid tissue primary culture buffer, and then the tissue is cut into pieces with ophthalmic scissors or a scalpel to a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human ovarian cancer primary tissue digestive fluid for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of human ovarian cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, human ovarian cancer organoid tissue primary culture buffer was added, and resuspended and centrifuged. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human ovarian cancer organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human ovarian cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add human ovarian cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human ovarian cancer organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid medium for human ovarian cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human ovarian cancer organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Human ovarian cancer organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human ovarian cancer organoid subculture buffer for resuspension and transfer. 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid medium for human ovarian cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146182219,"sku":"abs9641-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146214987,"sku":"abs9641-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-gastric-organoid-culture-medium-abs9642","title":"Mouse Gastric Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer for normal mouse gastric organoid tissue ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with primary culture buffer for normal mouse gastric organoids, the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal primary gastric tissue of mice for tissue use ( \u003cstrong\u003eabs9790\u003c\/strong\u003e ）   digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  The following cell clusters were followed by the addition of three times the volume of mouse normal gastric organoid tissue primary culture buffer to terminate digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse normal gastric organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 ）   Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse normal gastric organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse normal gastric organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e ）   digestion 2-3min Add normal mouse gastric organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of normal mouse gastric organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Mouse normal gastric organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal gastric organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Mouse normal gastric organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse gastric organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Mouse normal gastric organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse gastric organoid culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146575435,"sku":"abs9642-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146608203,"sku":"abs9642-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"pig-endometrial-carcinoma-organoid-culture-medium-abs9737","title":"Pig Endometrial Carcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Pre-cooled porcine endometrial organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with porcine endometrial organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Pig endometrial primary tissue digestive fluid for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of porcine endometrial organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and porcine endometrial organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and porcine endometrial organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of porcine endometrial organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add porcine endometrial organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of porcine endometrial organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Porcine endometrial organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of porcine endometrial organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Porcine endometrial organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of porcine endometrial organoid subculture buffer to resuspend and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Porcine endometrial organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146640971,"sku":"abs9737-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146673739,"sku":"abs9737-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-endometrial-carcinoma-organoid-culture-medium-abs9738","title":"Mouse Endometrial Carcinoma Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled mouse endometrial organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle was sterilized, and the tissue was placed in a petri dish. After washing three times with mouse endometrial organoid tissue primary culture buffer, the tissue was cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Mouse endometrial primary tissue digestive fluid for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of mouse endometrial organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and mouse endometrial organoid tissue primary culture buffer was added for resuspension and centrifugation. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse endometrial organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse endometrial organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add mouse endometrial organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse endometrial organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse endometrial organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse endometrial organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Mouse endometrial organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of mouse endometrial organoid subculture buffer to resuspend and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse endometrial organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146706507,"sku":"abs9738-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146739275,"sku":"abs9738-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-gliomatosis-cerebri-organoid-culture-medium-abs9740","title":"Human Gliomatosis Cerebri Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human glioma organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human glioma organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Human glioma primary tissue digestive fluid for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of human glioma organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human glioma organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human glioma organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human glioma organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add human glioma organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human glioma organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid medium for human gliomas. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human glioma organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Human glioma organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human glioma organoid subculture buffer to resuspend and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid medium for human gliomas. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224146935883,"sku":"abs9740-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224146968651,"sku":"abs9740-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-prostate-cancer-organoid-culture-medium-abs9742","title":"Human Prostate Cancer Organoid Culture Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human prostate cancer organoid tissue primary culture buffer #abs9731 Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human prostate cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human prostate cancer tissue for tissue use #abs9522  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was terminated by adding three times the volume of human prostate cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human prostate cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human prostate cancer organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Pipette and gently blow the matrix glue, collecting... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human prostate cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add human prostate cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human prostate cancer organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL Organoid medium for human prostate cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Pipette and gently blow the matrix glue, collecting... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human prostate cancer organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Human prostate cancer organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human prostate cancer organoid subculture buffer to resuspend and transfer 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL Organoid medium for human prostate cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147001419,"sku":"abs9742-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147034187,"sku":"abs9742-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-ovary-organoid-culture-abs9755","title":"Mouse ovary Organoid  Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 Normal ovarian tissue from mice after sampling must be in  2 - 8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer #9731  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 The tissue was placed in a petri dish, washed three times with primary culture buffer, and then cut into approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3 mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid of normal ovarian primary tissue in mice for tissue use #abs9522 digestion, 37℃ oscillation digestion 15-20 min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, digestion was stopped by adding three times the volume of primary culture buffer. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, primary culture buffer was added and resuspended for centrifugation. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25-30 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and mouse normal ovarian organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of mouse normal ovary organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520  digestion 2-3min Add normal mouse ovarian organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of mouse normal ovary organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse normal ovarian organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of mouse normal ovary organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution. #abs9519 Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Mouse normal ovarian organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of normal mouse ovarian organoid subculture buffer for resuspension and transfer. 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse normal ovarian organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147066955,"sku":"abs9755-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147099723,"sku":"abs9755-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-small-intestine-organoid-culture-abs9764","title":"Mouse(balb\/C) Small Intestine Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal small intestinal tissue in mice must be 2-8°C Wash, prepare several petri dishes, and add  4℃ pre-cooled Balb\/C Mouse normal small intestine organoid tissue primary culture buffer #abs9731 Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Place the tissue in a petri dish and utilize... Balb\/C After washing the primary culture buffer of normal small intestinal organoid tissue in mice three times, remove impurities, and then use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of mouse small intestine tissue was removed, and the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Primary tissue digestive fluid of normal small intestine tissue in mice #abs9644 digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 Take a small amount of liquid and observe it under a microscope. If more crypts are observed under the microscope, add three times the volume. Balb\/C Mouse normal small intestine organoid tissue primary culture buffer terminated digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Move back to remove the supernatant and add Balb\/C Mouse normal small intestine organoid tissue primary culture buffer was resuspended and centrifuged. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C Mice were cultured in normal small intestine organoid medium (restored to room temperature). \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount. Balb\/C Resuspension and transfer of organoid subculture buffer from normal small intestine of mice 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520 digestion 2-3min , add Balb\/C Mouse normal small intestine organoid subculture buffer was terminated for digestion and centrifuged. 5min Discard the mixture and add an appropriate amount. Balb\/C Resuspension and transfer of organoid subculture buffer from normal small intestine of mice 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C Mouse normal small intestine organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount. Balb\/C The mouse normal small intestine organoid subculture buffer was resuspended again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mlBalb\/C Mouse normal small intestine organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add appropriate amount Balb\/C Resuspension and transfer of organoid subculture buffer from normal small intestine of mice 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ulBalb\/C Mouse normal small intestine organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Small Intestine Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147132491,"sku":"abs9764-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147165259,"sku":"abs9764-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-colon-organoid-culture-abs9765","title":"Mouse(balb\/C) Colon Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal colon tissue in mice must be 2-8°C Wash, prepare several petri dishes, and add  4℃ pre-cooled Balb\/C Mouse normal colon organoid tissue primary culture buffer #abs9731 Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Place the tissue in a petri dish and utilize... Balb\/C After washing the primary culture buffer of normal colonic organoid tissue in mice three times, remove impurities, and then use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of mouse colon tissue was removed, and the tissue was cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Primary tissue digestive fluid of normal colon tissue in mice #abs9644 digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 Take a small amount of liquid and observe it under a microscope. If more crypts are observed under the microscope, add three times the volume. Balb\/C Mouse normal colon organoid tissue primary culture buffer terminated digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Move back to remove the supernatant and add Balb\/C Mouse normal colon organoid tissue primary culture buffer was resuspended and centrifuged. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C Mice were cultured in normal colon organoid medium (restored to room temperature). \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount. Balb\/C Resuspension and transfer of normal colon organoid subculture buffer in mice 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520 digestion 2-3min , add Balb\/C Digestion was terminated in mouse normal colon organoid subculture buffer and centrifuged. 5min Discard the mixture and add an appropriate amount. Balb\/C Resuspension and transfer of normal colon organoid subculture buffer in mice 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C Mouse normal colon organoid medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount. Balb\/C The mouse normal colon organoid subculture buffer was resuspended again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mlBalb\/C Mouse normal colon organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add appropriate amount Balb\/C Resuspension and transfer of normal colon organoid subculture buffer in mice 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ulBalb\/C Mouse normal colon organoid medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Colon Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147198027,"sku":"abs9765-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147230795,"sku":"abs9765-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"pig-lung-organoid-culture-abs9766","title":"Pig Lung Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer of normal porcine lung organoid tissue  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9731\u003c\/strong\u003e\u003c\/span\u003e Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with normal porcine lung organoid tissue primary culture buffer, and then the tissue is cut into approximately [volume missing] using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal pig lung primary tissue for tissue use  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9522\u003c\/strong\u003e\u003c\/span\u003e  digestion, 37℃ oscillation digestion 20-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of primary culture buffer for normal porcine lung organoid tissue. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and the primary culture buffer of porcine normal lung organoid tissue was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9495\u003c\/strong\u003e\u003c\/span\u003e  Re-hang the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and porcine normal lung organoid culture medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9730\u003c\/strong\u003e\u003c\/span\u003e placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of porcine normal lung organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9520\u003c\/strong\u003e\u003c\/span\u003e  digestion 2-3min Add normal porcine lung organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of porcine normal lung organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Porcine normal lung organoid culture medium. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of porcine normal lung organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  \u003cspan style=\"color: #e03e2d;\"\u003e\u003cstrong\u003eabs9519\u003c\/strong\u003e\u003c\/span\u003e Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Porcine normal lung organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of porcine normal lung organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Porcine normal lung organoid culture medium. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePig Lung Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147296331,"sku":"abs9766-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147329099,"sku":"abs9766-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fetal-rat-small-intestine-organoid-culture-abs9767","title":"Fetal Rat Small Intestine Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestine tissue of rat fetuses after sampling must be in 2-8°C Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer for normal small intestine organoids in rat fetuses #abs9731 Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 The tissue was placed in a petri dish, washed three times with primary culture buffer for normal rat fetal small intestinal organoid tissue, impurities were removed, the intestinal mucosa of the fetal rat small intestinal tissue was removed with ophthalmic scissors or a scalpel, and the tissue was cut into a volume of approximately...  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 Digestive solution of primary tissue of normal small intestine tissue of rat fetus for tissue use #abs9644 digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer for normal small intestinal organoid tissues of rat fetuses was added to terminate digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then the supernatant was removed, and the primary culture buffer of normal small intestine organoid tissue of rat fetus was added to resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and normal small intestine organoid culture medium of rat fetuses was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of normal small intestine organoid subculture buffer for rat fetuses, and resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520 digestion 2-3min Add subculture buffer for organoids from the normal small intestine of rats and fetuses to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of normal small intestine organoid subculture buffer for rat fetuses, and resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid culture medium in the normal small intestine of the rat fetus. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of rat fetal rat normal small intestine organoid subculture buffer for resuspension, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Rat fetal rat normal small intestine organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer for normal small intestine of rat fetuses for resuspension and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid culture medium in the normal small intestine of the rat fetus. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Small Intestine Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147394635,"sku":"abs9767-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147427403,"sku":"abs9767-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fetal-rat-colon-organoid-culture-abs9768","title":"Fetal Rat Colon Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 After sampling, normal colonic tissue from rat fetuses must be in 2-8°C Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer for normal colonic organoids in rat fetuses #abs9731 Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 The tissue was placed in a petri dish, washed three times with primary culture buffer for normal rat fetal colon organoid tissue, impurities were removed, the intestinal mucosa of the fetal rat colon tissue was removed with ophthalmic scissors or a scalpel, and the tissue was cut into a volume of approximately...  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 Digestive solution of primary tissue of normal colon tissue of rat fetus for tissue use #abs9644 digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer for normal colonic organoid tissues of rat fetuses was added to terminate the digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then the supernatant was removed, and the primary culture buffer of normal colon organoid tissue of rat fetus was added to resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and normal colon organoid culture medium of rat fetuses was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer #abs9730 placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of rat fetal rat normal colon organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution. #abs9520 digestion 2-3min Add rat fetal rat normal colon organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of rat fetal rat normal colon organoid subculture buffer, resuspend and transfer. 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Organoid culture medium for the normal colon of rat fetuses. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of rat fetal rat normal colon organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml Rat fetal rat normal colon organoid subculture buffer 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer for normal colon of rat fetuses for resuspension and transfer 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Organoid culture medium for the normal colon of rat fetuses. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Colon Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147460171,"sku":"abs9768-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147492939,"sku":"abs9768-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rat-small-intestine-organoid-culture-abs9769","title":"Rat Small Intestine Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestinal tissue of rats after sampling must be in 2-8°C Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer for normal rat small intestine organoid tissue ( abs9731 ) Standby (add bispecific antibodies and gentamicin). \u003cbr\u003e （ 2 The tissue was placed in a petri dish, washed three times with primary culture buffer for normal rat small intestinal organoid tissue, and impurities were removed. The intestinal mucosa of the rat small intestinal tissue was then removed using ophthalmic scissors or a scalpel, and the tissue was cut into a volume of approximately...  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 Primary tissue digestive fluid for tissue use in normal rat small intestinal tissue ( abs9644 Digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer for normal rat small intestine organoid tissue was added to terminate the digestion. \u003cbr\u003e （ 5 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and the primary culture buffer of normal rat small intestine organoid tissue was added for resuspension and centrifugation. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of normal rat small intestine organoid medium (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( abs9730 ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of rat normal small intestine organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( abs9520 Digestion 2-3min Add normal rat small intestine organoid subculture buffer to terminate digestion and centrifuge. 5min The mixture was discarded, and an appropriate amount of rat normal small intestine organoid subculture buffer was added for resuspension and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium in the normal small intestine of the rat. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of rat normal small intestine organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Rat normal small intestine organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of rat normal small intestine organoid subculture buffer for resuspension and transfer. 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium in the normal small intestine of the rat. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Small Intestine Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147525707,"sku":"abs9769-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147558475,"sku":"abs9769-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rat-colon-organoid-culture-abs9770","title":"Rat Colon Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original  \u003c\/strong\u003e\u003cbr\u003e （ 1 After sampling, normal rat colon tissue must be in 2-8°C Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer for normal rat colon organoid tissue ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Standby (add bispecific antibodies and gentamicin). \u003cbr\u003e （ 2 The tissue was placed in a petri dish, washed three times with primary culture buffer for normal rat colon organoid tissue, and impurities were removed. The intestinal mucosa of the rat colon tissue was then removed using ophthalmic scissors or a scalpel, and the tissue was cut into a volume of approximately...  10-30mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 3 Primary tissue digestive fluid for tissue use in normal rat colon tissue ( \u003cstrong\u003eabs9644\u003c\/strong\u003e Digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer for normal rat colon organoid tissue was added to terminate the digestion. \u003cbr\u003e （ 5 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and the primary culture buffer of normal rat colon organoid tissue was added for resuspension and centrifugation. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 double tissue volume matrix glue #abs9495 Re-hang the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of rat normal colon organoid medium (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e) placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of rat normal colon organoid subculture buffer, resuspend and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add normal rat colon organoid subculture buffer to terminate digestion and centrifuge. 5min The mixture was discarded, and an appropriate amount of rat normal colon organoid subculture buffer was added for resuspension and transfer. 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Organoid medium for normal colon in rats. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of rat normal colon organoid subculture buffer to resuspend again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution, gently blow and resuspend, and 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL Rat normal colon organoid subculture buffer 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of rat normal colon organoid subculture buffer for resuspension and transfer. 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Organoid medium for normal colon in rats. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Colon Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147591243,"sku":"abs9770-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147624011,"sku":"abs9770-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bladder-cancer-organoid-culture-abs9780","title":"Bladder Cancer Organoid Culture","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue was placed in a pre-cooled ( 2-8°C The tissue preservation solution was quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled human bladder cancer organoid tissue primary culture buffer ( \u003cstrong\u003eabs9731\u003c\/strong\u003e ) Backup. \u003cbr\u003e （ 3 The sample bottle is sterilized, the tissue is placed in a petri dish, washed three times with human bladder cancer organoid tissue primary culture buffer, and then the tissue is cut into a volume of approximately [size missing] using ophthalmic scissors or scalpels.  1-3mm\u003csup\u003e3\u003c\/sup\u003e Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human bladder cancer tissue for tissue use ( \u003cstrong\u003eabs9522\u003c\/strong\u003e Digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, digestion was terminated by adding three times the volume of human bladder cancer organoid tissue primary culture buffer. \u003cbr\u003e （ 6 ) Use 100μm  The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min The supernatant was then removed, and human bladder cancer organoid tissue primary culture buffer was added to resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization was performed, and human bladder cancer organoid medium was added (restored to room temperature) for culture. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL  4℃ Organoid subculture buffer ( \u003cstrong\u003eabs9730\u003c\/strong\u003e ) Place 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant, add an appropriate amount of human bladder cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestive fluid ( \u003cstrong\u003eabs9520\u003c\/strong\u003e Digestion 2-3min Add human bladder cancer organoid subculture buffer to terminate digestion and centrifuge. 5min Discard the mixture, add an appropriate amount of human bladder cancer organoid subculture buffer, resuspend and transfer. 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  Organoid medium for human bladder cancer. \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discarding the supernatant, adding an appropriate amount of human bladder cancer organoid subculture buffer and resuspending again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution ( \u003cstrong\u003eabs9519\u003c\/strong\u003e ), gently blowing and resuspending, to 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  Human bladder cancer organoid subculture buffer 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of human bladder cancer organoid subculture buffer to resuspend and transfer 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  Organoid medium for human bladder cancer. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBladder Cancer Organoid Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224147755083,"sku":"abs9780-100mL","price":476.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224147787851,"sku":"abs9780-500mL","price":1587.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/organoid-culture-medium.oembed?page=6","provider":"AntBio","version":"1.0","type":"link"}