{"title":"Organoid Culture Kit","description":null,"products":[{"product_id":"human-liver-organoid-culture-medium-kit","title":"Human Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human liver primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal liver organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal liver organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal liver organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Human normal liver organoid culture medium is a culture medium that promotes the formation and amplification of human normal liver organoids in vitro. This product is a sterile liquid mixture system containing amino acids, vitamins, organic and inorganic compounds, and growth factors essential for maintaining the growth of the target cells. The unique formulation of this medium can provide a suitable nutritional environment required by cells to promote the in vitro growth and expansion of normal human liver organoids. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 72.89%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e human normal liver organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e digestive fluid of human normal liver primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303119947,"sku":"abs9544-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ed1e9373-6caf-4e5f-bbb5-7f517693a8dc.jpg?v=1789743690"},{"product_id":"human-lung-organoid-culture-medium-kit","title":"Human Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human lung primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal lung organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal lung organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal lung organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303152715,"sku":"abs9546-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_7fd4d3e2-dbac-4768-bfe4-92858607270e.jpg?v=1789743694"},{"product_id":"human-pancreatic-organoid-culture-medium-kit","title":"Human Pancreatic Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary pancreatic tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal pancreatic organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal pancreatic organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal pancreatic organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal pancreatic organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal pancreatic primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303218251,"sku":"abs9547-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_c6f31fb2-49c1-4fa3-bfb1-dfe45eed441f.jpg?v=1789743698"},{"product_id":"mouse-intestinal-cancer-organoid-culture-medium-kit","title":"Mouse Intestinal Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse intestinal cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse intestinal cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse intestinal carcinoma organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse intestinal carcinoma organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse intestinal carcinoma organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse intestinal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303251019,"sku":"abs9548-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_9a291bc7-6d8e-4dff-84d7-311b08ac03ca.jpg?v=1789743700"},{"product_id":"mouse-gastric-cancer-organoid-culture-medium-kit","title":"Mouse Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse gastric cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse gastric cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse gastric cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse gastric cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse gastric cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse gastric cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303316555,"sku":"abs9549-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_8e817024-79e2-4e34-af72-6cbe24b5367b.jpg?v=1789743704"},{"product_id":"mouse-lung-cancer-organoid-culture-medium-kit","title":"Mouse Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse lung cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse lung cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL mouse lung cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL mouse lung cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 95%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse lung cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of mouse lung cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303349323,"sku":"abs9550-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_895d6cae-fb59-4b9b-96ae-391a9298ab9e.jpg?v=1789743706"},{"product_id":"mouse-breast-cancer-organoid-culture-medium-kit","title":"Mouse Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse breast cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse breast cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse breast cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse breast cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse breast cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse breast cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303414859,"sku":"abs9551-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e30b76f8-9149-4703-b339-a1cb12d8704c.jpg?v=1789743710"},{"product_id":"mouse-hepatocarcinoma-organoid-culture-medium-kit","title":"Mouse Hepatocarcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse liver cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of mouse liver cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse liver cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse liver cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse liver cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse liver cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303447627,"sku":"abs9552-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_4ef46df1-e4a1-488e-b156-a173aed77e75.jpg?v=1789743712"},{"product_id":"mouse-pancreatic-cancer-organoid-culture-medium-kit","title":"Mouse Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of pancreatic cancer in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse pancreatic cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse pancreatic cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse pancreatic cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse pancreatic cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse pancreatic cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303513163,"sku":"abs9553-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_429fbac4-fb30-47eb-8e0a-3235bffc5f8b.jpg?v=1789743717"},{"product_id":"bovine-mammary-glands-organoid-culture-medium-kit","title":"Bovine Mammary Glands Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary mammary tissue of dairy cows for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, adding dairy cow mammary gland organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul dairy cow mammary gland organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul dairy cow mammary gland organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e dairy cow mammary gland organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive juice of primary mammary tissue of dairy cow  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303873611,"sku":"abs9591-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/58322a37c6ab4b6eabc91acf0566c768.png?v=1789743739"},{"product_id":"mouse-gastric-organoid-culture-medium-kit","title":"Mouse Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary gastric tissue in mice for tissue use  C  digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal gastric organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL mouse normal gastric organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL mouse normal gastric organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Gastric Organoid Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse normal gastric organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal gastric primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303906379,"sku":"abs9643-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_107e2501-eee8-4b2c-9789-fadb19d9b1e3.jpg?v=1789743742"},{"product_id":"human-gliomatosis-cerebri-organoid-culture-medium-kit","title":"Human Gliomatosis Cerebri Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human glioma primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human glioma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human glioma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human glioma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human glioma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human glioma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303971915,"sku":"abs9739-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_39026509-37ed-4427-b68a-9d3375f896e6.jpg?v=1789743752"},{"product_id":"human-prostate-cancer-organoid-culture-medium-kit","title":"Human Prostate Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human prostate cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25 For μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human prostate cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human prostate cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human prostate cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human prostate cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human prostate cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304004683,"sku":"abs9743-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_12ef97d9-1d70-4d2a-8081-66f05b5e6272.jpg?v=1789743757"},{"product_id":"mouse-ovary-organoid-culture-kit","title":"Mouse ovary Organoid  Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 Normal ovarian tissue from mice after sampling must be in  2 - 8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3 mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid of normal ovarian primary tissue in mice for tissue use C digestion, 37℃ oscillation digestion 15-20 min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer B Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer B Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25-30 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal ovary organoid medium A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL mouse normal ovarian organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL mouse normal ovarian organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 64.8752%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse normal ovarian organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal ovarian primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304070219,"sku":"abs9754-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_f411da63-773c-42ce-8568-0fbe6988f7d0.jpg?v=1789743756"},{"product_id":"lung-cancer-like-organ-culture-medium-kit","title":"Human Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original :\u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human lung cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human lung cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human lung cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human lung cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Kit Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 157px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 10px; text-align: center;\"\u003e human lung cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 10px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e digestive fluid of primary tissue of human lung cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e organoid primary digestive juice  D\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8963%; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199930955,"sku":"abs9443-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92.jpg?v=1789743643"},{"product_id":"colorectal-cancer-organ-culture-medium-kit","title":"Human Colorectal cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human colorectal cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human colorectal cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human colorectal cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  human colorectal cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 80%; height: 154px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e human colorectal cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e Digestive fluid of primary tissue of human colorectal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 18px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 10px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199963723,"sku":"abs9445-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e413b8087fd94ae896ce7306dedca286.png?v=1789743648"},{"product_id":"breast-cancer-like-organ-culture-medium-kit","title":"Human Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human breast cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human breast cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human breast cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  human breast cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Product Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human breast cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human breast cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199996491,"sku":"abs9446-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8b75981794ca4051af93267e3e17a4d2.jpg?v=1789743649"},{"product_id":"pancreatic-cancer-organ-like-culture-medium-kit","title":"Human Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human pancreatic cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human pancreatic cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human pancreatic cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human pancreatic cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human pancreatic cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human pancreatic cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200029259,"sku":"abs9447-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_8475c546-8e74-4949-9862-f39c6d6870aa.jpg?v=1789743650"},{"product_id":"endometrial-cancer-like-organ-culture-medium-kit","title":"Human Endometrial carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human endometrial cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human endometrial carcinoma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human endometrial carcinoma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human endometrial carcinoma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human endometrial carcinoma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human endometrial carcinoma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200062027,"sku":"abs9448-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_78aca818-b84a-4e4b-a962-c080d84ce27e.jpg?v=1789743659"},{"product_id":"gastric-cancer-like-organ-culture-medium-kit","title":"Human Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissues of human gastric cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of human gastric cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human gastric cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human gastric cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human gastric cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human gastric cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200094795,"sku":"abs9449-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_0f7ddc16-a4a4-4ed3-be2d-31e78d2fa151.jpg?v=1789743658"},{"product_id":"mouse-intestinal-organ-culture-medium-reagent","title":"Mouse Intestinal Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestine tissue of mice after sampling must be placed in a precooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and stem cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities. Use ophthalmic scissors or a scalpel to remove the intestinal mucosa of the small intestine tissue and cut it into a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of normal small intestine in mice for tissue use  C  digestion, 4℃ oscillation digestion 10min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal small intestine organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organ collection, matrix gel was added for resuspension, and each well... 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  mouse normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  mouse normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Small Intestine Organoid Media Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Kit Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 164px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e mouse normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of normal small intestine in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 10px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 10px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518201700427,"sku":"abs9514-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_2280aaa3-aa55-4c77-b748-5793fa585adc.jpg?v=1789743682"},{"product_id":"mouse-lung-organ-culture-medium-kit","title":"Mouse Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary lung tissue in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Mouse normal lung organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Mouse normal lung organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Lung Organoid Culture Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Mouse normal lung organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal mouse lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202257483,"sku":"abs9515-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e23f9f8e-42ef-42cd-aaf9-65b0d9e20d49.jpg?v=1789743684"},{"product_id":"mouse-liver-like-organ-culture-medium-kit","title":"Mouse Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary liver tissue in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal liver organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse normal liver organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL mouse normal liver organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Liver Organoid Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 72.89%; height: 176px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e mouse normal liver organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal liver primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202290251,"sku":"abs9516-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_d68df51a-8f19-44c4-b049-02a72b9dd915.jpg?v=1789743689"},{"product_id":"ovarian-cancer-organoid-culture-medium-kit","title":"Human Ovarian Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human ovarian cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human ovarian cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human ovarian cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human ovarian cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human ovarian cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human ovarian cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202486859,"sku":"abs9558-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_855faaf4-d2ac-4ed3-ae50-780cdfd0f16d.jpg?v=1789743725"},{"product_id":"pig-endometrial-like-organ-culture-medium-kit","title":"Pig Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Pig endometrial primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of porcine endometrial organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul porcine endometrial organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul porcine endometrial organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e porcine endometrial organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Porcine endometrial primary tissue digestive fluid  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202552395,"sku":"abs9583-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_37608130-63fc-46ff-b362-7a6777fc40b3.jpg?v=1789743724"},{"product_id":"mouse-endometrial-like-organ-culture-medium-kit","title":"Mouse Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Mouse endometrial primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse endometrial organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse endometrial organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse endometrial organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse endometrial organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse endometrial primary tissue digestive fluid  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202617931,"sku":"abs9585-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_a53132b8-a6f3-43fb-a638-4532ba37a20c.jpg?v=1789743725"},{"product_id":"gastric-organoid-culture-medium-kit","title":"Human Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary gastric tissue for tissue use  C  digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal gastric organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal gastric organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal gastric organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal gastric organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal gastric primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202650699,"sku":"abs9586-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_07e81514-b3ef-4e30-81b7-1dfa9f08c803.jpg?v=1789743737"},{"product_id":"nasopharyngeal-carcinoma-organoid-culture-medium-kit","title":"Human Nasopharyngeal Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissues of human nasopharyngeal carcinoma for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human nasopharyngeal carcinoma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human nasopharyngeal carcinoma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human nasopharyngeal carcinoma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human nasopharyngeal carcinoma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e digestive fluid of primary tissue of human nasopharyngeal carcinoma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202683467,"sku":"abs9589-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_0d2365dc-a096-4022-84d1-e0240da77bf0.jpg?v=1789743731"},{"product_id":"organotial-cervical-cancer-organoid-culture-medium-kit","title":"Human Cervical Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human cervical cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of human cervical cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human cervical cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human cervical cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human cervical cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human cervical cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202978379,"sku":"abs9590-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_73c3d55a-1ff4-48ce-a6c4-da1a293ca610.jpg?v=1789743737"},{"product_id":"mouse-small-intestine-organoid-culture-kit","title":"Organotial Balb\/C小鼠正常小肠类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal small intestinal tissue in mice must be  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After cleaning three times, remove impurities and use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of the mouse small intestine tissue was removed and sheared to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Digestive fluid of primary tissue of normal small intestine in mice  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added. B Termination of digestion. \u003cbr\u003e （ 5 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C mouse normal small intestine organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C mouse normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Balb\/C mouse normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003eBalb\/C mouse normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003eBalb\/C Digestive fluid of primary tissue of normal small intestine in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204125259,"sku":"abs9757-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_21f6cbe3-2c00-4a36-b735-ec1fa1207947.jpg?v=1789743759"},{"product_id":"mouse-colon-organoid-culture-kit","title":"Organotial Balb\/C小鼠正常结肠类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal colon tissue in mice must be  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After cleaning three times, remove impurities and use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of the mouse colon tissue was removed and sheared to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Digestive fluid of primary tissue of normal colon in mice  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C mouse normal colon organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C mouse normal colon organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ulBalb\/C mouse normal colon organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBalb\/C mouse normal colon organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBalb\/C Digestive fluid of primary tissue of normal colon in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204158027,"sku":"abs9758-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_9285d95a-1a7d-4432-8f51-6bed9bb7ad04.jpg?v=1789743765"},{"product_id":"pig-lung-organoid-culture-kit","title":"Pig lung Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal pig lung primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 20-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of porcine normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Porcine normal lung organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Porcine normal lung organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePig lung Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Porcine normal lung organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of normal porcine lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204190795,"sku":"abs9759-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_52b7d571-c166-4854-a135-039e81ba9d34.jpg?v=1789743766"},{"product_id":"fetal-rat-small-intestine-organoid-culture-kit","title":"Fetal Rat Small Intestine Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestine tissue of rat fetuses after sampling must be in  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to remove the intestinal mucosa of the fetal rat small intestine tissue, shearing it into a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive solution of primary tissue of normal small intestine of rat fetus for tissue use  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of rat fetal normal small intestine organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Rat fetal rat normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Rat fetal rat normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Rat fetal rat normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of normal small intestine of rat fetus  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204223563,"sku":"abs9760-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_7fac4fd7-4f94-4740-bc22-17f471e432ed.jpg?v=1789743768"},{"product_id":"fetal-rat-colon-organoid-culture-kit","title":"Fetal Rat Colon Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 After sampling, normal colonic tissue from rat fetuses must be in  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to remove the intestinal mucosa of the fetal rat colon tissue and cut it into a volume of approximately...  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid of primary tissue of normal colon of rat fetus for tissue use C digestion, 4℃ oscillation digestion  20-25min  Or allow to stand and digest.  40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of rat fetal rat normal colon organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Rat fetal rat normal colon organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Rat fetal rat normal colon organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFetal Rat Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Rat fetal rat normal colon organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of normal colon of rat fetus  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204256331,"sku":"abs9761-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_667bdd16-a65e-4c5e-a916-ea0a6432e226.jpg?v=1789743773"},{"product_id":"rat-small-intestine-organoid-culture-kit","title":"Organotial大鼠正常小肠类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestinal tissue of rats after sampling must be in  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer.  B  After washing three times, impurities were removed, and the intestinal mucosa of the normal small intestine tissue of the rat was removed using ophthalmic scissors or a scalpel, shearing it to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid of primary tissue of normal small intestine in rats for tissue use  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of normal rat small intestine organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Rat normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Rat normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Rat normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of normal small intestine in rats  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206386251,"sku":"abs9762-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_d3a6451b-7134-40af-a8ed-fa2c93ebadd5.jpg?v=1789743776"},{"product_id":"rat-colon-organoid-culture-kit","title":"Organotial大鼠正常结肠类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 After sampling, normal rat colon tissue must be in  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to remove the intestinal mucosa of the normal colon tissue of the rat, shearing it to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid from primary tissue of normal colon in rats for tissue use  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of rat normal colon organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Rat normal colon organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Rat normal colon organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Rat normal colon organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of normal colon in rats  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206419019,"sku":"abs9763-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_1053edfc-07dc-4383-96af-5dbbc5f20535.jpg?v=1789743786"},{"product_id":"esophagus-cancer-organoid-culture-kit","title":"Esophagus Cancer Organoid  Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human esophageal cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human esophageal cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human esophageal cancer organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human esophageal cancer organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Kit Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 176px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e human esophageal cancer organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human esophageal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.5424%; height: 22px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 19.7234%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206484555,"sku":"abs9773-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_73931175-8190-40d7-bdf6-489e3954a5f8.jpg?v=1789743784"},{"product_id":"bladder-cancer-organoid-culture-kit","title":"Organotial人膀胱癌类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human bladder cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human bladder cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human bladder cancer organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human bladder cancer organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBladder Cancer Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human bladder cancer organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human bladder cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206517323,"sku":"abs9778-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_167c6a35-7ba5-479f-a0ba-426048ae2803.jpg?v=1789743787"},{"product_id":"pig-hepar-organoid-culture-kit","title":"Organotial猪正常肝类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive solution of normal liver primary tissue of pigs for tissue use  C  digestion, 37℃ oscillation digestion 15-30min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of porcine normal liver organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL Porcine normal liver organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL Porcine normal liver organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePig Hepar Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Porcine normal liver organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e digestive fluid of normal liver primary tissue in pigs  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206550091,"sku":"abs9779-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_d5c9819e-5b3d-4908-b2e0-c932151166b3.jpg?v=1789743791"},{"product_id":"icc-organoid-culture-kit","title":"Organotial人肝胆管癌类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of human hepatocholangiocarcinoma for tissue use  C  digestion, 37℃ oscillation digestion 15-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of human hepatocholangiocarcinoma organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL human hepatocholangiocarcinoma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL human hepatocholangiocarcinoma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eICC Organoid  Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human hepatocholangiocarcinoma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e digestive fluid of primary tissue of human hepatocholangiocarcinoma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206779467,"sku":"abs9786-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_70140f02-51c6-4c33-8071-8d28874f4079.jpg?v=1789743791"},{"product_id":"esophagus-organoid-culture-kit","title":"Organotial人正常食管类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from human normal esophageal primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 20-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal esophageal organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL Human normal esophageal organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL Human normal esophageal organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEsophagus Organoid  Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Human normal esophageal organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal esophageal primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518206812235,"sku":"abs9787-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_9a65aee4-6792-4d11-9024-20afbe7c4fcf.jpg?v=1789743795"},{"product_id":"renal-cell-carcinoma-organoid-culture-kit","title":"Organotial人肾癌类器官培养试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 Organizational recommendations after data collection are as follows: 2-8°C Stored under conditions, quickly transported to a clean laboratory for tissue processing and cell separation, photographed and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human renal cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 15-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Re-hang, 300g enrichment centrifugation 5min Move back to Shangqing. \u003cbr\u003e （ 7 The cell pellet contained red blood cells. Discard the supernatant and add... 1-2mL erythrocyte lysate 1-2min Then, dilute to 10mL ， 300g centrifuge 5min 。 \u003cbr\u003e （ 8 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 9 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL-30uL Tissue matrix glue mixture for board laying ( \u003cstrong\u003e Note: \u003c\/strong\u003e Matrix adhesive maintained 0-4℃ (Next operation). \u003cbr\u003e （ 10 Place the prepared culture plate in... 37℃ Incubator 10-15min After the matrix gum sets, add... 500-750uL human renal cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10-15min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids: 300g centrifuge 5min Discard the supernatant and add 1mL organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number of organoids is large or the volume is large: 300g centrifuge 5min Discard the supernatant and add 1-2mL organoid passage digestive fluid  D  digestion 2-4min , add 3 Organoid subculture buffer with twice the volume of organoid digestive fluid  G  Termination of digestion, 300g centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Abandon the Shangqing and proceed with the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min 。 After the matrix gum sets, add to each well 500-750uL human renal cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 ) each 3 Hole addition 2mL organoid cryopreservation fluid  F Gently blow and mix well, then transfer to cell cryopreservation tubes. Each tube... 1mL 。 \u003cbr\u003e （ 5 After marking the information, performing program cooling, move to... -80°C In the refrigerator, 48h Then, it was transferred to liquid nitrogen for long-term storage. Or put in 4℃ refrigerator 40min Then, put in -20℃ in the refrigerator 2h , move to -80℃ In the refrigerator, 48h Afterwards, it was placed in a liquid nitrogen tank for storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube should be gently shaken to ensure that the cryopreservation solution is completely thawed in a short time. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend, move in 1.5mL centrifuge tube 300g centrifuge 5min They abandoned the Shangqing \u003cbr\u003e （ 5 ) Add per cryopreservation tube 100ul Matrix gel resuspension, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500-750uL human renal cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human renal cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human renal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518207762507,"sku":"abs9835-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ecc32432-91e5-4094-b72b-11efbadcc5bf.jpg?v=1789743800"},{"product_id":"carcinoma-of-the-larynx-organoid-culture-kit","title":"Carcinoma of the larynx  Organoid  Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e With 24 Aperture plate as an example \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e I. Sample Pre-Processing \u003c\/strong\u003e\u003cbr\u003e1.  Experimental materials: Primary culture buffer B （ 4 ℃ Pre-cooling), tissue preservation solution E Sampling tubes, tissue transport boxes, ice packs \u003cbr\u003e2.  Sample collection and transportation: The tissue should be in vitro. 30 min Place tissue preservation solution inside E , primary culture buffer B cleaning 3-5 Next, the blood on the tissue surface is washed clean and placed in tissue preservation solution. E In the sampling tube 4 ℃ Cryogenic storage and transportation ( 72 h inside). \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 2. Primary culture of organoids \u003c\/strong\u003e\u003cbr\u003e1.  Experimental Materials: \u003cbr\u003e （ 1 Materials required: Matrix glue (in advance) 24 h Put in 4 ℃ (Refrigerator melting), tweezers ( 10 cm ), pointed ophthalmic surgical scissors \/ Surgical blade, disposable 60 mm Petri dishes, 1.5 mL\/15 mL\/50 mL EP tube, 100 um a cell screen, 3 mL pasteurized straw \/1000 uL pipetting gun, 24 Well cell culture plate, metal ice box. \u003cbr\u003e （ 2 Kit Reagents: Primary Culture Buffer B （ 4 ℃ ), primary tissue digestive juice C （ 37 ℃ ), human laryngeal cancer organoid culture medium A (Room temperature or 37 ℃ ）。 \u003cbr\u003e2.  Organoid Construction: \u003cbr\u003e （ 1 The laryngeal cancer tissue after sampling is recommended to be...  2-8 ℃ Store and transport under these conditions, and quickly transfer to a clean laboratory for the experimental process of constructing human laryngeal cancer organoids. Organize photography and register detailed information. \u003cbr\u003e （ 2 After disinfecting the sampling tube, the tissue was removed from the ultraclean bench, placed in a petri dish, and primary culture buffer was added. B , use 3 mL pasteurized straw or 1000 uL Pipette gun blow cleaning, repeat cleaning operation 3 Number of times or more. \u003cbr\u003e （ 3 Use ophthalmic scissors or a surgical blade to remove tissue impurities, and transfer the forceps to... 1.5 mL EP In the tube, ophthalmic scissors are used to further mechanically dissociate the tissue into a volume of approximately  1-3 mm\u003csup\u003e3\u003c\/sup\u003e The tissue block was transferred to 15 mL EP In the tube, add 5 mL Digestive fluid of primary tissue of human laryngeal cancer C 37 ℃ oscillation digestion 15-25 min 。 Each digestive process 10 min The tissue digestion was observed under a microscope. A small amount of digestive juice was taken and observed under the microscope, and a larger amount was observed. 70 um The following cell clusters or individual cells are followed by the next step. \u003cstrong\u003e Note: \u003c\/strong\u003e If the amount of tissue is too small or the biopsy tissue is used 1 mL Digestive fluid of primary tissue of human laryngeal cancer C In 1.5 mL EP Tubular digestion. \u003cbr\u003e （ 4 Digestion is completed and the tissue passes 100 um The cell sieve with a pore size is filtered, the filtrate is collected, and added... 3 Double volume of primary culture buffer B  Rinsing terminates digestion. 300 g enrichment centrifugation 5 min Abandoning the supernatural; If the cell pellet contains red blood cells, add 1-2 mL erythrocyte lysate 1-2 min Then, dilute to 10 mL ， 300 g enrichment centrifugation 5 min They abandoned their superiors. \u003cstrong\u003e Note: \u003c\/strong\u003e Tumor organoid culture is performed directly when there is too little sedimentation or no red blood cells. \u003cbr\u003e （ 5 Observe the volume of cell pellets collected by centrifugation and add... 25 Double the volume of matrix glue is resuspended, forming 3D Cultivate the spatial structure to avoid bubbles during the resuspension process. The cell pellet volume is shown in the figure below. Add the cell pellet volume according to the figure. 200 uL 、 150 uL 、 100 uL 、 50 uL Matrix glue. \u003cdiv style=\"text-align: justify;\"\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/318431bcb65e4dc9a8be5eed4bcf2962.png\" alt=\"\" width=\"250\" height=\"127\"\u003e\u003cbr\u003e\u003cp style=\"text-align: justify;\"\u003e Example: 24 well cell culture plate according to 25 uL-30 uL\/ hole   Dispensing glue, \u003cstrong\u003e matrix rubber \u003c\/strong\u003e\u003cstrong\u003e whole course \u003c\/strong\u003e\u003cstrong\u003e Maintain 0-4\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003e℃ operation under conditions \u003c\/strong\u003e 。 cell culture plate placement 37 ℃ Incubator 10-15 min After the matrix gum has set, add to each well. 500-750 uL  human laryngeal cancer organoid culture medium A (in advance) 37 ℃ (Preheating) for cultivation. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e III. Organoid subculture \u003cbr\u003e\u003c\/strong\u003e1.  Experimental Materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e （ 1 Materials required: Matrix glue (in advance) 24 h Put in 4 ℃ (Refrigerator melting) 1.5 mL\/15 mL EP tube, 3 mL pasteurized straw \/1000 uL pipetting gun, 24 Well cell culture plate, metal ice box. \u003cbr\u003e （ 2 Kit Reagents: Organoid Subculture Buffer G （ 4 ℃ ), organoid passage digestive fluid D （ 37 ℃ ), human laryngeal cancer organoid culture medium A (Room temperature or 37 ℃ ）。 \u003cbr\u003e2.  Organoid Passage: \u003cbr\u003e （ 1 Select appropriate organoids for passage, generally after about a week of growth, under a microscope. 10X You can see more below 20 Organoids, or size 100-200 um Organoids. Aspirate off the medium and add an equal volume of organoid subculture buffer to each well G The pipette gently blows away the matrix glue, collecting it from... 15 mL EP In the tube, each 6-8 The hole is transferred to one EP tube, 4 ℃ standing 10-15 min 。 \u003cbr\u003e （ 2 Whether digestion and passage are necessary is determined based on the growth of the organoid. \u003cstrong\u003e Note: \u003c\/strong\u003e If there is little precipitation at the bottom of the tube after centrifugation, no cells are seen, and the matrix glue is not delaminated, it can be resuspended again to increase the centrifugal force and centrifuge again. \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003ea:  When the number of organoids is insufficient or the volume is small, 300 g centrifuge 5 min Abandon the Shangqing Dynasty \u003cbr\u003eb:  When the number of organoids is high or the volume is large, 300 g centrifuge 5 min Discard the supernatant, and you can choose digestive juice digestion or mechanical digestion. \u003cbr\u003e     Digestive juice digestion: Add 1-2 mL organoid passage digestive fluid D After the cell pellet is blown out, it is digested at room temperature. 2-4 min Blow every minute and observe under a microscope until digested (Figure). A-B It can be stopped when in the ) state. Add 3 Organoid subculture buffer with twice the volume of organoid digestive fluid G  Termination of digestion, 300 g centrifuge 5 min Abandon the Shangqing Dynasty \u003cbr\u003e    Mechanical digestion: Add 1 mL organoid subculture buffer G ， 1 mL pipetting gun blowing 20-30 Time, until the blowing and beating reach (picture). C-D It can be stopped and added when in the ) state. 10 mL passage buffer, 300 g centrifuge 5 min Abandon the Shangqing Dynasty \u003c\/p\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240411\/76fb16898e414dc1a8ef51ee427fc05b.png\" alt=\"\" width=\"387\" height=\"233\"\u003e\u003cbr\u003e （ 3 Observe the volume of organoid pellets collected by centrifugation. If there is very little pellet, it can be reserved. 1 Dispense the supernatant with twice the precipitation volume; a large amount of precipitated supernatant can be absorbed. Add... 25 The organoids were resuspended in an amount of matrix gel twice the organoid pellet volume. Example: 24 well cell culture plate according to 25 uL-30 uL\/ hole   Dispensing glue, \u003cstrong\u003e The matrix adhesive is maintained throughout the entire process. 0-4 ℃ operation under conditions \u003c\/strong\u003e 。 cell culture plate placement 37 ℃ Incubator 10-15 min After the matrix gum has set, add to each well. 500-750 uL  human laryngeal cancer organoid culture medium A (in advance) 37 ℃ (Preheating) for cultivation. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e IV. Cryopreservation of organoids \u003c\/strong\u003e\u003cbr\u003e1.  Experimental Materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e （ 1 Materials required: 15 mL EP Tubes, cell cryopreservation tubes, programmed cooling cassettes, and pipetting guns. \u003cbr\u003e （ 2 Kit Reagents: Organoid Subculture Buffer G （ 4 ℃ ), organoid cryopreservation solution F 。 \u003cbr\u003e2.  Cryopreservation of organoids: \u003cbr\u003e Organoids that are temporarily unused should be frozen and stored in a low-temperature environment. \u003cbr\u003e （ 1 Aspirate off the culture medium, and add an equal volume of organoid subculture buffer to each well. G The pipette gently blows away the matrix glue, collecting it from... 15 mLEP In the tube, each 6-8 The hole is transferred to one EP tube, 4 ℃ standing 10-15 min 。 300 g centrifuge 5 min Discard the supernatant and add it every three holes. 2 mL organoid cryopreservation fluid F Gently blow and mix well, then transfer to cell cryopreservation tubes. Each tube... 1 mL 。 \u003cbr\u003e （ 2 Mark the information, place it in the program cooling box, and move it to... -80 ℃ In the refrigerator, 48 h Afterwards, it was placed in a liquid nitrogen tank for storage. Or put in 4 ℃ refrigerator 40 min Then, put in -20 ℃ in the refrigerator 2 h , move to -80 ℃ In the refrigerator, 48 h Afterwards, it was placed in a liquid nitrogen tank for storage. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e V. Organoid resuscitation culture \u003c\/strong\u003e\u003cbr\u003e1.  Experimental Materials: \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e （ 1 Materials required: Matrix glue (in advance) 24 h Put in 4 ℃ (Refrigerator melting) 15 mL EP Pipe, water bath, pipette gun, 24 Well cell culture plate, ice box. \u003cbr\u003e （ 2 Kit Reagents: Organoid Subculture Buffer G （ 4 ℃ ), human laryngeal cancer organoid culture medium A 。 \u003cbr\u003e2.  Organoid resuscitation culture: \u003cbr\u003e （ 1 The frozen organoids were removed from the cryogenic environment and quickly placed in... 37 ℃ The cryopreservation tube needs to be gently shaken during the water bath thawing process to ensure that the cryopreservation liquid is completely thawed in a short time. Rapid transfer of thawed organoids to 15 mLEP Gently blow on the tube using a pipette. 6-8 next time, 300 g  centrifuge 5 min Abandon the Shangqing; Add an appropriate amount of passage buffer G Resuspend, move in 1.5 mL EP tube 300 g centrifuge 5 min They abandoned the Shangqing \u003cbr\u003e （ 2 ) Add per cryopreservation tube 100 uL The matrix glue is resuspended, 24 well cell culture plate according to 25 uL-30 uL\/ hole   Dispensing adhesive, the matrix adhesive is maintained throughout 0-4 ℃ Operate under these conditions. cell culture plate placement 37 ℃ Incubator 10-15 min After the matrix gum has set, add to each well. 500-750 uL  human laryngeal cancer organoid culture medium A (in advance) 37 ℃ (Preheating) for cultivation. \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human laryngeal cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human laryngeal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Human laryngeal cancer organoid culture medium A can be stored at 4℃ for 3 months. After receiving the goods, it is recommended to use it within 1 month. If not used for a long time, it is recommended to store it at -20℃ to avoid repeated freezing and thawing more than twice.\u003cstrong\u003e\u003cstrong\u003e。\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e2. Tissue preservation solution E and human laryngeal cancer primary tissue digestive solution C contain nutrients that maintain cell activity. In order to maintain the activity of the reagent nutrients, it is recommended to store them at -20℃ without long-term use to avoid repeated freezing and thawing more than twice.\u003cbr\u003e3. Thaw the matrix glue overnight at 2–8℃ ambient conditions. When using matrix glue, keep it in an ice box to prevent premature setting. The matrix gel formed a gel within 20 min at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518207795275,"sku":"abs9858-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ace31fd8-02b6-4d84-a4dd-9b47e55f71c5.jpg?v=1789743803"},{"product_id":"pyrophosphatase-inorganic","title":"Pyrophosphatase, Inorganic","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eYeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eInorganic pyrophosphatase、PPase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.1U\/μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e33.5 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e[1] Lahti R , Pitk RantaT , Valve E ,et al. Cloning and characterization of the geneencoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal ofBacteriology, 1988, 170(12):5901-7\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e[2] Satoh T , Watanabe M ,Nogi S I ,et al. Molecular Cloning, Expression, and Site-DirectedMutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journalof Biochemistry, 1998, 124(1):79-88.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003ePyrophosphatase, Inorganic is a yeast derivedinorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is anenzyme that catalyzes the conversion of one molecule of pyrophosphate to twomolecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis toform phosphates. In molecular biology, it can be used to increase theproduction of RNA in vitro transcription reactions.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStorage Solution: 0.1U\/μl\u003cspan style=\"color:black\"\u003e Pyrophosphatase, Inorganic\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e、\u003c\/span\u003e\u003cspan style=\"font-size:10.5pt\"\u003e 20mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50%Glycerol \u003cspan style=\"color:black\"\u003e(pH 8.0 @ 25°C)\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eUse 1-3 units per mlin a high yield in vitro RNA synthesis reaction \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM PPi).\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10U","offer_id":41518782578763,"sku":"UA070038-10U","price":40.0,"currency_code":"USD","in_stock":true},{"title":"50U","offer_id":41518782611531,"sku":"UA070038-50U","price":80.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/65facccf-40f2-4510-a036-d41e372495fb.png?v=1787623336"},{"product_id":"lung-organoids-cytokine-set-human","title":"Lung organoids Cytokine Set, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePulmonary organoid cytokine kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 792.41px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 202.669px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eContain\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 199.007px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eReference dosage\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 134.002px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eS size\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 129.009px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eM size\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 127.005px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eL size\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eActivin A,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e100ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e10ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e50ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e100ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eFGF- basic,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e50ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e5ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e25ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e50ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eFGF-4,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e250ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e25ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e125ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e250ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eNoggin,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e200ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e20ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e100ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e200ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eWnt Surrogate,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e100ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e10ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e50ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e100ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 238.553px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003eEGF,Human\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:12.0000pt\"\u003e\u003cfont\u003e20ng\/ml\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e5ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.576px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e10ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 238.588px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e20ug\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519874965579,"sku":"UA090014-S","price":630.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519874998347,"sku":"UA090014-M","price":1790.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519875031115,"sku":"UA090014-L","price":3145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_54160b7e-0b76-48aa-9964-14f2b1722a7d.png?v=1789113656"},{"product_id":"mammary-organoids-cytokine-set-human","title":"Mammary organoids Cytokine Set, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBreast organoid cytokine kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eS-10μg\u003cbr\u003eM-50μg\u003cbr\u003eL-100μg\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519875063883,"sku":"UA090025-S","price":880.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519875096651,"sku":"UA090025-M","price":2245.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519875129419,"sku":"UA090025-L","price":3790.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_b8def03a-daa2-4028-bf9a-b26a531b68c5.png?v=1789113653"},{"product_id":"small-intestinal-and-colonic-organoids-cytokine-set-human","title":"Small intestinal and colonic organoids Cytokine Set, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSmall Intestine and Colon Organoid Cytokine Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eS-10μg\u003cbr\u003eM-50μg\u003cbr\u003eL-100μg\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519875260491,"sku":"UA090029-S","price":350.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519875293259,"sku":"UA090029-M","price":905.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519875326027,"sku":"UA090029-L","price":1495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c8effe1f-40fc-429a-8f1c-6ac3396e4407.png?v=1789113652"},{"product_id":"retina-organoids-cytokine-set-human","title":"Retina organoids Cytokine Set, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRetinal Organoid Cytokine Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eS-10μg\u003cbr\u003eM-50μg\u003cbr\u003eL-100μg\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519875358795,"sku":"UA090028-S","price":245.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519875391563,"sku":"UA090028-M","price":700.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519875424331,"sku":"UA090028-L","price":1145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_02b68a1a-e01e-4fc2-83e0-a086d07c5a4b.png?v=1789113649"},{"product_id":"prostate-organoids-cytokine-set-human","title":"Prostate organoids Cytokine Set, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProstate organoid cytokine kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eS-10μg\u003cbr\u003eM-50μg\u003cbr\u003eL-100μg\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519875457099,"sku":"UA090027-S","price":530.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519875489867,"sku":"UA090027-M","price":1265.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519875522635,"sku":"UA090027-L","price":2185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1f0e8d6d-72b9-4439-a924-004198e16ab2.png?v=1789113646"},{"product_id":"organogel-s","title":"Standard OrganoGel with Phenol red","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSee the operating manual for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe basement membrane is a layer of matrix membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of the matrix glue are: laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, the matrix glue also contains various growth factors, such as epidermal growth factor, nerve growth factor (Vukicevic et al. 1992).\u003cbr\u003e\u003cstrong\u003eProduct characteristics:\u003c\/strong\u003e\u003cbr\u003eThis product is liquid at 4°C, but gels when heated to 37°C. After the matrix gum has set, it is placed back at 4° C. overnight and the matrix gum can be liquefied again.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emouse tumor basement membrane components\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis matrix begins to solidify at temperatures above 10°C, so it is best to handle the matrix on ice. During organoid passage, in order to avoid enzymes affecting the organoids, the matrix gel can be slowly blown with 4°C pre-cooled basal medium to release the organoids from the matrix gel.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is recommended that users repackage the product according to a single dose after the first thaw and store it in a refrigerator at -80°C. It has a shelf life of 2 years.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is suitable for two-dimensional and three-dimensional culture, cell invasion, cell migration experiments, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1.5mL×4","offer_id":42969159172171,"sku":"abs9490-1.5mL×4","price":189.0,"currency_code":"USD","in_stock":true},{"title":"1.5mL×8","offer_id":41683173146699,"sku":"abs9490-1.5mL×8","price":378.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/57358c4c78f349f9832c2195f5695e3c.jpg?v=1789743661"}],"url":"https:\/\/www.antbioinc.com\/collections\/organoid-culture-kit.oembed?page=7","provider":"AntBio","version":"1.0","type":"link"}