{"title":"Magnetic-Bead Cell Separation","description":null,"products":[{"product_id":"starter-magsep-separator-l-s0d3001","title":"Starter MagSep Separator (L)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807340619,"sku":"S0D3001-1pc","price":870.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4e98b27a03a64378afbc1724cdc8bfff.png?v=1788080444"},{"product_id":"starter-magsep-separator-4l-s0d3002","title":"Starter MagSep Separator (4L)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807373387,"sku":"S0D3002-1pc","price":1875.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2c519df4aea041ef82c29d7e92c66909.png?v=1788080446"},{"product_id":"starter-magsep-separator-s-s0d3003","title":"Starter MagSep Separator (S)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15–35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSeparators and columns are sensitive to heat and aggressive solvents, such as acetone. Do not autoclave separators or the MultiStand. Separators and the MultiStand can be cleaned with a soft cleansing tissue and a mild detergent, and disinfected using 70% ethanol. Do not drop Separators. Do not use if damage is visible.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807406155,"sku":"S0D3003-1pc","price":360.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4b0750454b1b417f8a24dadfc209425c.png?v=1788080447"},{"product_id":"starter-magsep-separator-8s-s0d3004","title":"Starter MagSep Separator (8S)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15–35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807438923,"sku":"S0D3004-1pc","price":1425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6b48891127ef403ca1660786f66c3588.png?v=1788080449"},{"product_id":"starter-magsep-separator-l-kit-s0d3006","title":"Starter MagSep Separator (L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" src=\"data:image\/png;base64,iVBORw0KGgoAAAANSUhEUgAAA9sAAABVCAYAAABZ2\/fpAAAgAElEQVR4Ae2dz6stV5mG808IjgM2PQrajpQLjoJIFEEJISMFceYgdBy00mqgmwxEUAeKiiBNYwbOlFzIqDuN4yAEh8Fh4jzOT\/Pu5Dn3Pd9dq2rtffau2lXnLThZVau+X+tZ31r1rXtukmdubm5u3n\/\/\/fyEQXIgOZAcSA4kB5IDyYHkQHIgOZAcSA4kB86UA8\/ooJ0rBPZIILm9x1nNmEIgBOYIZO+bI5T3IRACIRACIXB5Avoe57B9ec7xsBKBFJwrgY\/bEAiBVQlk71sVf5yHQAiEQAiEwIFADttJhF0TSMG56+nN4EIgBDoEsvd1wKQ7BEIgBEIgBBYkkMP2grDjankCKTiXZx6PIRAC6xPI3rf+HCSCEAiBEAiBEMhhOzmwawIpOHc9vRlcCIRAh0D2vg6YdIdACIRACITAggRy2F4QdlwtTyAF5\/LM4zEEQmB9Atn71p+DRBACIRACIRACOWwnB3ZNIAXnrqc3gwuBEOgQyN7XAZPuEAiBEAiBEFiQQA7bC8KOq+UJpOBcnnk8hkAIrE8ge9\/6c5AIQiAEQiAEQiCH7eTArgmk4Nz19GZwIRACHQLZ+zpg0h0CIRACIRACCxLIYXtB2HG1PIEUnMszj8cQCIH1CWTvW38OEkEIhEAIhEAI5LCdHNg1gRScu57eDC4EQqBDIHtfB0y6QyAEQiAEQmBBAjlsLwg7rpYnkIJzeebxGAIhsD6B7H3rz0EiCIEQCIEQCIHJw\/Zf3nnn5rlPPXvz7Cc\/cfvz9S9\/6eYfH374FLnHf\/rjrYx0pOtXyxZ2f\/6TH7vo7f13vv2tSZsSnPM7KoNT+ezFg0za7RDoFZytfGzltuR++6tf3nvA57LjgTCG1npDTmtV49Ja01o590UMrGW1LY7n9uv2riEGj+c+95fIE49He5v2OF1w6+WF3j\/\/6PM3f\/\/gAzeR+40Q6O19hJ9vHSTShkAIhEAIhMDlCHQP2xxiayGmD\/TnPv3cnQJMMl7w12eF3yvsVMjJHgUgQ61+WjZrX32WrdpXn\/GHrA4LOWw7lW3ftwpO5UDr8Flzjty8bz6cy06dCdbUVM66jMZ9zmuU4zl9VlvXEEON6dTnS+UJ8dTDM7kxlRd+OMdO2m0QaO19RM66ue\/ehr20IRACIRACIRACbQLdw7YOHvUALBO1IOQ3Z\/WjXfWnCrv6rj7Lb\/VTnxme+x2RQU\/x69AydXBBNu12CLQKTs8RH0nN7frsssfcn8tO9ck6+eIXHnV\/m6y8fvTZzxz+MGzqUFVtjzyPchyxdarMNcRwauxV71J5Ij+tvZD8mcoLYpqSqePI83UQaO19iizfuuuYn0QRAiEQAiHwMAg0D9sUZipk565ewaYPuv8GvCeHffma+uunNaaePfc7IiP\/KiT1m\/m3Hr95iFk2cu2DQC04ax71Rskhgz+A8dxUrtJf\/3AGPZf5l3\/+p1t5t+NFr+z4gaZlx98rbvL79dd+dMhbPfulsX7jpRdvfvebXz912Pb46hiw4TJay7LDmh7liC21I+PFB3yn1uKaMTibym9q7np66DDuqTxxJui53ZonYq\/c0Nx5jpA\/LXmfN9nWT65tEah7n6LXXE9968inkXVY13NyZFv5kWhDIARCIASWIdA8bMs1H9K5QqxVxElfehTmep4r7OTP5evw9d7\/qvqI3xEZ90OhIV+59kGgVXCO5nYrH1RQ+kFIea4DEusEHc9l+jyvWvms\/EYGHbdTZ4Q19Yc3fn+ICV3kFJNi\/fPbb985bM+NQfqScd+M0\/vkz8eO31Y7Ol63x\/jquNz+GjHM8evN3aiej1c6dd87Nk\/ESzY9b9UHX3LXufq93vu8+7vcXy+B1t5HtOSo55re0T+3Duv+gJ76c4VACIRACIRACDwh0D1sS4RCVh9e\/\/g+UX\/yJ+Uq3PxSgdYqEnuFXa+g8xhct9rHt\/f7Pe\/V9vopGGoB4rq53xaBXsHpedXL7ZoPPCt\/uOgjZ3j2opO+KsMztvTMoQYdt4McrR+WpFsPU9JVv8thd2oMLo8vtbJHfPTLPvvDKEfXxR5x1fE6E\/Rqu2QMxDnFDxkfC30jehqPrt48OBPsuq\/Kp\/c3AHr2q\/6oXNXL87oEenufoiJvyDUipb\/mUyvnPJfRTxsCIRACIRACIXCXwORhG1GKNYpq\/xDrg+uHanRq\/1zBJnkKb2x4iz6+q31kvd\/vea+210+hUQsQ1839tghMFZwayVRuj+SDckXrgpxp6dS+Xv4px7UG1FadFnXWhOy5rmSlr\/+StPpdrmWnjkHPrbUoP63+OY7HjFeyftVx+bt6PzWXl4yh8huZO8U+pzcVs\/ZcvR\/x1ZOZywv49vR5n\/Y6CUztfb05pX9qHfby8jopJKoQCIEQCIEQWJfA0GHbQ6RA49DbK4b1QfbCHL36Ecd2r8DnvVrJcLAf8Tsi4\/YpNOQn1z4ITBWcdYTkKLndygflL3\/opPbFr7xwyHNypqVT+6oNt0d+V50aq56JV\/Y4aBK7+vhNt8tJr\/qvY+itRen5mm7F5HF5LD5Gv6\/jlQ+\/emvYZVr3jPkSMczx683dsXo93oxN73u+nElPxu24fL3v6Ve5PF8Xgam9rzen9Cu3\/PJ12MtLl899CIRACIRACITARwSOPmxLTQVsr5AHbC3Y5wo72aQwxkZt9ZGnOO\/Zc78jMu6DQoODk7\/L\/TYJTBWcrRF5btd8IJ88P6pMfZaP2ud53IqhpdOSIx4KYy+CNQ7idDnuedfypXetQ7Xbb8Xjfc7xmPEyFmyN6CJb20vEMMKvzrfiOkWvN3bZ0vyobfmqHHoyxFSZj+pXuTxfF4Gpva+XE\/TXnPBc9PvrGnGiCYEQCIEQCIHrI9A8bM8VYSpi9aOL36h58a5+l9HzlM36Th9z\/farfvD9EDDid0TmMIiP\/0GhUcfiMrnfFoFacNZcq6PxvK35oHysh1DskTNVR\/ZrHzo1vz2WquPvuK920NF\/SZi\/Qi5ZlxsZg+Q5zOFLrdgwfrfpMtw7xzlZ6RC79PwSV\/5gz\/t1P2f3EjGM8GMs5IRiPUWvNz7ZZR5avion9sHKtme\/6o\/KVb08r0ug7n0eTS9v6K+54usw+eAkcx8CIRACIRAC0wSah22p6GPLb5HdhD60tRBXIemy9Vn6vQ906+NOcehFtmzqAF4L2Dm\/NZb67GMjFvfh73O\/PQKtgnM0t8lD8qHmMO+VlxSnrRxCDjuiqHvPXfXJxjGHqBoPNhSPrx2X83vJE5uPATvEomfWn\/eNcpT+6HgVh3zpqrEeOss\/lo6hxtTi18qBET1seZ7U8WEHmZavgujwKHnPCXViC94tPfXpvc97Ty7910WgtfcRYS9v6J9bh75XySa5W3MMf2lDIARCIARC4KES6B62BYQCWx9efnofUxVzyNRDhGxR2CHjLYWjTwIfb5drFYVzfmVzREZyFBqteDy23G+HQK\/gHM1tcofDBs\/kpez4gaiXQ+hhRwTpw5avrZ4dJ8+a8nXBuGSbq8pVv3UM6GlcxKa4+X\/vKjYu\/CGn1seBnNrq1+UY7\/e\/++rhYIc9H5vb8vulY6jjqPwYi89Ba\/xVz2Wm8sSZ9Hw5H90rB2RTLRd5AWtvPXblgX5ybYtAb+\/TKHp5Q\/\/IOqzrIDmyrfxItCEQAiEQAssQmDxsLxNCvITA5QhMFZyX87pPyyqu\/YB8zlFS5Psh75z2R2xdQwwjcZ4iwx9eHssXJn7AP8V\/dJYncMrex3wfmyfLjy4eQyAEQiAEQmAbBHLY3sY8JcoTCZxScJ7oajdqOljpt5x+wLp0EX5p+yOTcw0xjMR5qox+k61\/l1\/jHL106MpvLEdpXZfcKXvf3tfAdc1QogmBEAiBEHgIBHLYfgiz\/IDHeErB+YBx3Q6dA7f\/1WI\/fN8KnunmGor8a4jhTDi7Zo45PJ9yOO86zovFCZyy9z2ENbD4RMRhCIRACITAgyZwe9jWTX7CIDmQHEgOJAeSA8mB5EByIDmQHEgOJAeSA+fJgWcEMlcI7JFAcnuPs5oxhUAIzBHI3jdHKO9DIARCIARC4PIE9D3OYfvynONhJQIpOFcCH7chEAKrEsjetyr+OA+BEAiBEAiBA4EctpMIuyaQgnPX05vBhUAIdAhk7+uASXcIhEAIhEAILEggh+0FYcfV8gRScC7PPB5DIATWJ5C9b\/05SAQhEAIhEAIhkMN2cmDXBFJw7np6M7gQCIEOgex9HTDpDoEQCIEQCIEFCeSwvSDsuFqeQArO5ZnHYwiEwPoEsvetPweJIARCIARCIARy2E4O7JpACs5dT28GFwIh0CGQva8DJt0hEAIhEAIhsCCBHLYXhB1XyxNIwbk883gMgRBYn0D2vvXnIBGEQAiEQAiEQA7byYFdE0jBuevpzeBCIAQ6BLL3dcCkOwRCIARCIAQWJJDD9oKw42p5Aik4l2cejyEQAusTyN63\/hwkghAIgRAIgRDIYTs5sGsCKTh3Pb0ZXAiEQIdA9r4OmHSHQAiEQAiEwIIEctheEHZcLU8gBefyzOMxBEJgfQLZ+9afg0QQAiEQAiEQApOH7b+8887Nc5969ubZT37i9ufrX\/7SzT8+\/PApco\/\/9MdbGelI16+WLez+\/Cc\/dtHb++98+1uTNiU453dE5u8ffHDzuU8\/d+uLuOQ\/17YJ1IKTPGzlKCNVfivPlQfKr7mL\/CGP5eO3v\/rlQa2+wxZxkGtqW2vLbaF7iVbjnGJyCZ+xGQIhcDkCde+rnvR9Y8+q7\/IcAiEQAiEQAiFwHgLdwzaH2HrY0AdaB1MdIrhqoV6fJcfhotrjMFIPttVPy2btq8\/yW\/vqM7E9\/+jzd8bE2NJum0AtOMlDHW57habLKF\/mLnJY9vy+pyebrYN8zfkRWz0fx\/a31sWxNiIfAiFwPQTq3ueRsQf19kCXzX0IhEAIhEAIhMDpBLqHbRX+9QAsN\/UAwG8B60e76nOAaR1e6rv6LL\/VT30GgfsdkZGeYmr9VhGbabdLoBac5NYXv\/CoO+fK5Uef\/czhN72tfK00fE34fZXj2XOUPrVVtz677LnvNc78ZvvcVGMvBNYjUPc+ItH+xt+oqd9tZNKGQAiEQAiEQAich0DzsM0htXXYrm45vNRDiT7i\/hvwnhz25GvqwFtj6tlzvyMy8t87\/BBb2u0SqAUnOfH6az865Kee\/VKefeOlF29+95tf3x62e4de+l9\/7YcHW7QUssrnv7333uEdRW3NY\/ft99h2W9LVpXylXy229Q69P7zx+8N6cjlfo25D65TxVh4eU+5DIAS2Q6DufYqcP1R76\/Gbd\/YlRsX+of3A\/9Uq32OQVZ\/vL9pTcoVACIRACIRACNwl0DxsS4QPqRfod1U\/elJxro9yLdKld8xhW\/5cvvrSe\/\/N24jfERkOP998+aWDfYoH+cu1fQK14FROKI84jNZ5Vt7qkPznt98++rAtWxSr2K3PIqp3yrO5tdXSVUHrfyglG24LHe\/DJ+tHNnytYYP325\/1jCAEQqDufU6EfYJ9inf0+\/7BnumydQ9BLwduSKYNgRAIgRAIgY8IdA\/bes2hQB9e\/\/g6PBXqrSK99vPBVn\/rUr8fAJDxGFy32kfe+\/2e92q9nyLBDzDE6sWF6+d+OwRqwcncKgc0vz7vGpWKRfW7HDlS84F+fqOt9\/QhW58hp\/esq97aqro8K3Yu+qq\/WvQi94uf\/fT2DxGwoVbyrXXsMrkPgRDYDoG693nk7AfsG7yjv+4fkuP7jIzvQ+inDYEQCIEQCIEQuEtg8rCNKL\/95XDgH2I\/uCKvtvb74cXluJc8H3P6vEUf39U+st7v97xX2+t3GS8uvD\/32yJQC07ySDmge+WcWl0qIvUfytOzy1Fc9grTUw7bUJxaWz2\/6KpVTFqXxNbToV\/rp7XORtaE+819CITAdROoe59Hy37AvsE7+rUf+OV7ZfYKJ5P7EAiBEAiBEJgmMHTYdhMcQjj0+kfY5fRB9qIevfoRR0cffZen31vJ8Nu3Eb8jMm7f71NQOI3t3teC0\/OQgy65rDnnN90uRwHaK0zvc9h2svgknpZfxcgfeql98SsvHNYNsbV05IP+l7\/21eY6S777TOQ+BLZPoO59PiL2A\/YN3tGv\/cAv\/5bq3dy32nVzHwIhEAIhEAIPmcDRh23B0mGgdShxkPqI+weZg0T9iKMjmxwy6KutHwh69tzviEz1wbP7oi\/t9gjUgrPmhBeOyj+KT5ejAOUdFOg\/12Fbdn1tYR+\/xMSz5KtMfa6xyr6vS94n3yGRNgT2QaDufT6quX1C+4Ffvj\/4vcvkPgRCIARCIARC4GkCzcM2RX394KKugl0\/uvjtoB8A1O8yep6yWd\/Jr35rV\/3LBweFEb8jMtX3YVAf\/\/VcfNGXdnsEasFZ55uiU\/\/1Xf4KuUbpcsjUHEfmmMM2OjW3IevrpvqVTs1J7BFb1cEu\/d979ZXmv7Mtff7WCDppQyAEtkug7n0+EvYD9g3e0c\/3nX7Jzf0BO7JpQyAEQiAEQiAEnhBoHrb1Wh\/bVvGt4l4Fv1ouHQJctj5LjkNBPWS0Pu4ckvm4S196\/u+m0jfnt8ZSn\/Hlhxh8qc21bQK14GzloXJdueX5VuUk4zlC3krPD9vkE0UscjyL5ujaqrZqTLxXDLKpq+Wv9isWHwv57mvpYCz\/CIEQ2CyBuvf5QOb2Ce0pfP\/qviM7dT9kL\/I91P3lPgRCIARCIAQeKoHuYVtAKML14eWn9zFVAY9Mq2jng42Mt34QYSL4eLscH39k1M75HZEZ9eV+c78NArXgJA89l8hzz8MqV3NEOc7\/q9YP255vOtD+9d13Dwdbty0ZfHp+t9YW+c3hmGf0ZMcP73NFNHG4HdnO\/2d7G\/mcKENglEDd+1xvbp\/4\/ndfPexbvs+4vu59D5Ecf+BX5fIcAiEQAiEQAg+ZwORh+yGDydj3QWCq4NzHCDOKEAiBEHiawCl7X+8Q\/rT19IRACIRACIRACIwQyGF7hFJkNkvglIJzs4NN4CEQAiHwMYFT9r4ctpM+IRACIRACIXBeAjlsn5dnrF0ZgVMKzisbQsIJgRAIgaMJnLL35bB9NOYohEAIhEAIhMAkgdvDtm7yEwbJgeRAciA5kBxIDiQHkgPJgeRAciA5kBw4Tw48I5C5QmCPBJLbe5zVjCkEQmCOQPa+OUJ5HwIhEAIhEAKXJ6DvcQ7bl+ccDysRSMG5Evi4DYEQWJVA9r5V8cd5CIRACIRACBwI5LCdRNg1gRScu57eDC4EQqBDIHtfB0y6QyAEQiAEQmBBAjlsLwg7rpYnkIJzeebxGAIhsD6B7H3rz0EiCIEQCIEQCIEctpMDuyaQgnPX05vBhUAIdAhk7+uASXcIhEAIhEAILEggh+0FYcfV8gRScC7PPB5DIATWJ5C9b\/05SAQhEAIhEAIhkMN2cmDXBFJw7np6M7gQCIEOgex9HTDpDoEQCIEQCIEFCeSwvSDsuFqeQArO5ZnHYwiEwPoEsvetPweJIARCIARCIARy2E4O7JpACs5dT28GFwIh0CGQva8DJt0hEAIhEAIhsCCBHLYXhB1XyxNIwbk883gMgRBYn0D2vvXnIBGEQAiEQAiEQA7byYFdE0jBuevpzeBCIAQ6BLL3dcCkOwRCIARCIAQWJJDD9oKw42p5Aik4l2cejyEQAusTyN63\/hwkghAIgRAIgRCYPGz\/5Z13bp771LM3z37yE7c\/X\/\/yl27+8eGHT5F7\/Kc\/3spIR7p+tWxh9+c\/+bGL3t5\/59vfmrQpwTm\/t8Zubm5kr+cLuREZZNNeP4FWwUkuKnce0qVx\/\/ZXvzwM+e8ffHDzuU8\/N7sezsUHf6x5tXNrseUbO6fotuylLwT2SqC19+nbrW\/41DpkjbmM7vVt7F2n7qnoua9ejdHzfS392V+vZSbOE4fqg1YtO2qddZRv1c3hPEDtMcqvylWe9bnKX8vzJeO8b45eC6OHEEf3sM0hth5I9MFVka4E4qoTXp8lx0e12iMR64e8+mnZrH31mfjU6p0+6FMb34iM28z99RNoFZy9XLz+0ZweIetsKv9Ptz6tKZ9ae1pfXMRzbGGN3hrjIPa0IbAFAnXv46Dt3+\/WN0\/74\/OPPn\/nGz833lP2VHz7viA\/9ds\/5\/sa3q+5L2V\/vUwGKC9z2L4\/20utjUvZvf+I71q4ZJz3zdG7kebpkgS6h2198OoBWIHUxOEDXovfqj\/1Ma7v6rP8Vj\/1GUjVr\/r5GE0dtkdk8JF2OwRqwanIW\/m1nRGdFmldt6dZOV5rijXv6t4x5WWtcUzFlHchcI0E6t6n9aaDtlq\/tP7qAfzYPwRjLdeDs\/up961vtWS2uMbXinmKO++yv9bMG3u+70FmrZwYG91yUpficCm75yZzyTjvm6PnHmvs9Qk0D9scZFuH7WqKDb1+ZOsHvCeHPfma+sDXmHr2ql+S8a3Hb3b\/2uyIDHGm3RaBWnAq+l7uTI0MHf66oxen6Cn3eN\/7Te7vfvPrQx4i1yqEtBZ4r9Zl2LhdhrXnfa6HDja1zv723nvN9VDH0PL9hzd+\/9RfRSUGWNBOrWut6f\/73\/9B9LYdiUEyjMtjlBH6X3\/th4cxVuaKCRmYYIP+qTGO5MLtYHITAisRaO19rVC0dn0\/0\/rQzzEXa6K3D1Rb9Xte37ee677gvli3da2zrt2exsa6931SMthxGfx4n+uhg83sr8vvr7258Tmd2tMl5za0HpRLvd9sM+dT+YaMctDvPRfp51t1Soy+dmV7ZJ34WJXfrF9yuNr0mFv3PX3Gh12v8Y+N843\/\/q87NQu2fY1P2axxoz81h+g4L1\/7vFdbfUtHF35acbb6YMW+4z48jl6O1jhadtxm7pch0DxsyzUTNjdRWmSadLV+Sc8XLIuxZ0\/+XN5tEY9vfKN+sdNKeN7Rjsggm3YbBFoF51wu1pG15LXp1Q9HzU89s5mSW9pIWQPYRUZ+q13Jug526loZ1cMXdnjG97FjkJ5suB78KKjdB+96rcbhtiojj9vv3R79FDBuT7GIZ+3jGV1nLtuMUcWQZJlDvavsPZbch8BaBFp7XysWz1\/W7DdffumQ51oH+plbw6xTXxctX97HWhzRYf3Jjy78EVdr3VYZ6flY9Zz99aP\/Lk\/lqGeY8u4A3g4P17K\/js5pb09XnsiGf1PJC74LjJ0WNm6z5hsyx7B0e\/IlXWLoxehxu7z0ezG5DjK+DitTxt1q5\/SdA\/qnxFnt1Oc5m\/imRd+ZMxbZ4qosyI3Ky5liW7rcY7PGKT+1rxeH+yAO8mPUDuNKuyyB7mGbiVMi8uPJRZjq88nu9ZM8LRvSUb8nEnaUhC3\/o36xUxOefm9HZFw+99dPoFVwzuViHVUv15Dr5Y1yl5xGRpuvXy0ZXyPoSU4Xz26HvhG9aofnHpNWfO7bY3L\/3o+PwwAm\/nFMDLLJuKt9+ikGPV58uA59ih9d1\/Gx\/OcPf9Dc7yaGlVchsAqB1t5XA1HOe7FJ\/vsfJLI+fM1UO8jUPaDK1WfZ5PvucbgcMVX\/es7+Ov4fuezNUYuj+nrc6b+G\/ZVYPO\/oI1947u3pv\/jZTw97uttQ\/km+VdvqXc\/mOVm6n6kY6xpg3KyhVkzOQuPujRMbU+2cPqyIqz5jey7OqufPfo89tW7T+3WPjrOoOsh4btBXx+My7svlpVP3OX\/veh57b+16jo7Ycfu5X5bA5GGbUPjTbj6Knpy9hVb7e8mCD8mzadDnLfr4rvaR7fX3EhE9tSMyLp\/76yfQKjjJJeXKyEVeKP\/ZYF2vl3Pyo5xWi43q02XcJvdszvjFDs\/I1XZOr9qZGoM+xHpfdfB5bD96tT02hjm\/FIPOCh2fB+\/j3nUUJ\/3Y7OVCHVOeQ2AtAq29z2PRGlAe8031d\/Ve62Hk++zrqtqYej6lxvC9k\/VZ\/btMy\/\/cPtnSUd+cHvGwjyiu1oFG8WV\/Pe\/+Ojc3zClzpPxv5XZvzqSP7lS+IaN4\/B7\/bofvCvmCDHovf+2r3RiJvRevrwHsuR\/6Tv2mzenzHp\/3jRM7bnfEJkxp0Z+aQ2S9lX9n1fONDn6++IVHd\/R439P3eZMM84yeWtf1e5dxO96f+2UJDB22PSQ+DnygexNZkwM99bcuJXArmVxWMnywRv2iT8KzUOn3dkTG5XN\/\/QRaBedcLrZGVYtBbbbkslo9t37IV3ILHXzUPK62XvzKC4d1Qd5ih2fsHKtX7Ui\/tf6cVdXBd68fZjVW9Gp7bAw9v\/S3ChjeyReX93FfY\/Z+xuXz7fawmzYE1iTQ2vuIR\/mt\/OU7Tn+vVX6zl7VkfJ9ovT+mD1vEJt++1vyemFifdR1mf31CXmyO2eNh2tsLr2V\/rfkx+s1kfFMHWfLrCcWP7tCdyjdkxM\/v3Rb9LZaS4\/1UjMxp5TC1Tuqc3vebNqXPGPB5apzVjj+P2HTuznZqDiVXbdf80nvmoPpwP8ql11\/70VP7aLXfmjexa\/mQLjk6YqcVX\/qWIXD0YVth6SPIXzPjw6iJ9qsmR08OHdnk40pfbT2xevaqX2z4wqSvtiMyVSfP102gVXD2cmd0JHxYfJPjvmeD3KrrpJXTfJRkCz366rNkGA8yI3rVjsfhY5BtbfJqqw5yvX69970CeW\/9\/bEx9PzS3ypgeOfz4H3cO0vF2+uvueBjy30IrEmgtfcpHq05FXQ1x6di7a1NdNiDfF3x7pR2ZF9wu6zP6t\/jJkYfN3r01Wf5OEWv2vE4PG7Zzv56+v56ytzAnzlSrs0dZNChRXcq35BRbvk9NtTS3\/pW+fvRw\/ZoHUK+eyzc3\/ebVvUZIz57awH\/Pm50Wn1ud8Sm23d7U3M4kl9zvj1O2HB+Uhxz+sjM5eiIncogz8sRaB62SbCahISlzUk\/ukgeXxTqdxk9T9ms7+RXxUD1Lx8k3KjfQ5C2qdU4ea\/WF4X35367BFoFZ823U0ZHrihHR+whz7rBp\/KRjVe2yJpaV4oAAAR7SURBVG\/eY5u8xQ7PkjtFr9rBz9SaqzrE2OvX+55df8dYerJ6Dxf35ffE4nZbBQw6Pk7v456YsNvr13veuU300obAWgRae5\/yeuqgPbIGW+Pp6bVk1Tcn7\/XDnKzssQazvz4hDjf2Mp7rPrXl\/VVj4dvAyBkn4yY3eEaO\/u+9+srht4MtLr3DK7pT+YaM\/Po9\/tUSa+tbpffoKUaNU\/J+yT\/jx1Ydh8tjr7JwGd0jN2Wr6viz63OPz1PjrHb8ecSmx+djnJrDkfya8+1xym+Vr881zpYOMmJKjo7YQS\/t8gSah22FoQRkEj0sTWhd9EpIl63P0u8lAonoCc9BmkOI9GWzFgnVT332uPHDgvd33I\/IIJt2GwRaBWcvF3sjauWV8oiPnPR808OOfwjJLf9DpBpHfWYdSIf1gR3P4xE9bKHXslPXPHandDTWli0YwMbH7Tq+xtV\/bAzO2O3KX6uAIVbNKZf3cc+Yq0yNT+8l67mATtoQWJNA3fvqem7Fxj7h+ay1Utdv1cW2r6sqU59ba0kyslVrDK0xrzEk52ufdetx1pjqM2OVjmzpwo6v\/xE9bKHXslPHi90pnV5Mh2A\/\/of0fdyus\/f9FYbkHfMwN6fOSPz008r5mnNwZ36de40FGebX89X9y0brW+UyslH1WZcet+RqzK5XY5IP2ak6lQfjbrVz+swJHGTjlDhr7PV5zmaNHf2pOaxzylg8v2TXGesZOa2\/1v9mtcqPxC4Zn2vm3+duxE7lkOdlCHQP23LPZCqx+KmbN2FqkpHxyec9SYuMt9KtF8nqcoqnXnN+kWdhtXwdI4Ns2m0QqAWnoj42F6VT10Irxz0Xlbe+Vsi\/73\/31cOGSV7XnK429F4bM\/6wU\/N4Tk9jQEYb9l\/fffcQx4gdZrrnu9ePntoW8+obeeJsMaq+6j4hTm89fvMwtlYBg75z9z7ua2zeP5ILjCVtCKxFoO59dV2xvtR6EVfXlN77emmNp7W+sV\/XkuvXtSQd3zddtsbvcqzP7K9ParUe98rR5xaO6NZcuMb9tTWekW9mHavb0XoY+f9sT+VbtX8sS+V+taFxsa6I0deudHwcdT1Ve6yvug6pN3gvm77e6Kcd0VcsHuuxcdbY67NimbJJrLToT81hy6bG6vmFvepbMrrwo\/dc9CGj\/qrf4u0yzH9rrsiROv\/4T7s8gcnD9vLhxGMInJdALTjPa33cGpurb7jj2pEMgRAIgeMIXMved1zUp0lnfz2NW7ROI3At+aZ6onUoO21UfS39QcF\/\/ODfD7+x7Utt6821zOG2qCXaUwnksH0quehtgsC1FJzZ2DeRLgkyBHZD4Fr2viWAZn9dgnJ8QGDpfOM3x2q5loxBf3Pl3\/71FVzvol2S3y6AZRD3IpDD9r3wRfnaCVxLwZmN\/dozJfGFwL4IXMvetwTV7K9LUI4PCKyRbxy4\/a8I++Gb2C7R6jfoS\/m6RPwtm2vMYSuO9D0MAreHbd3kJwySA8mB5EByIDmQHEgOJAeSA8mB5EByIDlwnhz4f4oFM4pb+8xlAAAAAElFTkSuQmCC\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807504459,"sku":"S0D3006-1pc","price":1040.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_588a79a7-e287-4e9f-8156-ead7fb7f8ab4.png?v=1788519637"},{"product_id":"starter-magsep-separator-4l-kit-s0d3007","title":"Starter MagSep Separator (4L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" src=\"data:image\/png;base64,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\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807537227,"sku":"S0D3007-1pc","price":1995.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_50c7baf8-de2f-4440-a235-f5490542de38.png?v=1788519639"},{"product_id":"starter-magsep-separator-s-kit-s0d3008","title":"Starter MagSep Separator (S) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" src=\"data:image\/png;base64,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\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807569995,"sku":"S0D3008-1pc","price":556.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9c383302-ff3b-4f85-a60a-8a56f7951ba1.png?v=1788519639"},{"product_id":"starter-magsep-separator-8s-kit-s0d3009","title":"Starter MagSep Separator (8S) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" src=\"data:image\/png;base64,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\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807602763,"sku":"S0D3009-1pc","price":1568.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d81648b6-d92c-45fc-b0c4-117ce7920d2a.png?v=1788519639"},{"product_id":"starter-magsep-separator-sl-kit-s0d3010","title":"Starter MagSep Separator (S\u0026L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" src=\"data:image\/png;base64,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\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807635531,"sku":"S0D3010-1pc","price":1382.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_380968f8-29de-4f39-a4bd-ebc6b2885548.png?v=1788519635"},{"product_id":"cd4-nanobeads-mouse-ruo-s0k0003","title":"CD4 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads conjugated with an anti-mouse CD4 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD4⁺ cells are magnetically labeled using CD4 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD4⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD4⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD4⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD4⁺ cells must be subjected to a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD4 antigen is expressed on most thymocytes and mature helper T cells, certain NKT cell subsets, and a small fraction of dendritic cell subsets. In CD4\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ehelper T cells, CD4 acts as a co-molecule to assist the TCR heterodimer in recognizing MHC class II\/peptide complexes. CD4 antigen is present on approximately 90% of thymocytes, 25% of splenic cells, and 55% of lymph node cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517841995,"sku":"S0K0003-1ml","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":42301372727371,"sku":"S0K0003-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ebe3156a992c4fb586128e55fa1492fb.jpg?v=1788094853"},{"product_id":"cd14-nanobeads-humanruo-s0k0004","title":"CD14 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD14 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD14⁺ cells are magnetically labeled using CD14 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD14⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD14⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD14⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD14 Nanobeads are used for the positive selection or depletion of human monocytes and macrophages from umbilical cord blood or peripheral blood mononuclear cells (PBMCs), as well as from pleural effusions, ascitic fluid, synovial fluid, or various tissues such as the spleen and lymph nodes. The CD14 antigen is part of the LPS receptor complex. Because CD14 lacks a cytoplasmic domain, antibody binding to CD14 does not trigger signal transduction. CD14 is strongly expressed on most monocytes and macrophages and weakly expressed on neutrophils and some myeloid dendritic cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":43030517743691,"sku":"S0K0004-1mL","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42301380984907,"sku":"S0K0004-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/53dd2b10efc44282bb4ec12673057a19.jpg?v=1788112965"},{"product_id":"cd8-nanobeads-mouseruo-s0k0005","title":"CD8 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with anti-mouse CD8 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD8⁺ cells are magnetically labeled using CD8 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive fraction containing CD8⁺ cells must be further separated through a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eCD8 Nanobeads\u003c\/span\u003e\u003cspan\u003eThese beads are designed for positive selection or depletion of murine cytotoxic T cells from lymphoid organs, blood, or tissues. The magnetic beads react with the immunoglobulin-like domain of the CD8α chain. The CD8α chain is expressed as a heterodimer with CD8β on cytotoxic T cells that express either the T cell receptor (TCR) α\/β or TCR γ\/δ, as well as on most thymocytes. As a homodimer composed of two CD8α chains, the CD8 antigen is present on most NK cells, the majority of intraepithelial lymphocytes in the intestine, some activated CD4+CD8+ T cells, and on CD8+ T cells in thymus-deficient mice.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517612619,"sku":"S0K0005-1ml","price":533.33,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42301424074827,"sku":"S0K0005-2mL","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/79082a3bd2a0436d8e65a20921136259.jpg?v=1788130962"},{"product_id":"cd19-nanobeads-humanruo-s0k0006","title":"CD19 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD19 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD19⁺ cells are magnetically labeled using CD19 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD19⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after depletion of CD19⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD19⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD19⁺ cells should be subjected to a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD19 Nanobeads are designed to isolate human B cells based on the expression of the CD19 antigen. CD19 is a 95–120 kDa glycosylated transmembrane protein that plays a crucial role in the signal transduction pathways regulating B lymphocyte development, activation, and differentiation. CD19 is expressed on cells of the B-cell lineage, from the early committed pro-B cell stage through the pre-B cell stage, including most malignant B cells. Its expression is downregulated during differentiation into plasma cells. In addition, CD19 is also expressed on follicular dendritic cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":42301461790795,"sku":"S0K0006-1ml","price":285.0,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":42301461823563,"sku":"S0K0006-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bab27e7feca64df394d24dfb4d989dfa.jpg?v=1788141868"},{"product_id":"anti-f480-nanobeads-mouseruo-s0k0012","title":"Anti-F4\/80 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnets conjugated with an anti-mouse F4\/80 monoclonal antibody, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, allowing up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, allowing up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eUsing Anti-F4\/80 Nanobeads, mouse anti‑F4\/80\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells are subjected to magnetic labeling. Subsequently, the cell suspension is loaded into a separation column positioned within the sorter's magnetic field. The magnetically labeled F4\/80…\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells remain retained within the column, while unlabeled cells elute; this fraction constitutes the F4\/80-depleted fraction.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eThe negative fraction following cell sorting. After removing the sorting column from the magnetic field, the F4\/80‑positive cells that were magnetically captured…\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells can be eluted to obtain the positive‑selection fraction. If higher purity is required, it contains F4\/80.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eThe positively sorted cell fraction must be subjected to a second round of sorting on a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eF4\/80 is a member of the epidermal growth factor (EGF) transmembrane 7 (TM7) family, consisting of a seven-transmembrane molecule and a large extracellular domain containing multiple EGF-like modules. F4\/80 is considered one of the most specific cell surface markers of murine macrophages. Constitutive high expression of F4\/80 is observed on the surface of most tissue-resident macrophages, including splenic macrophages, microglia in the brain, Kupffer cells in the liver, and Langerhans cells in the skin. Furthermore, F4\/80 expression is also regulated according to the physiological state of the cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302112301131,"sku":"S0K0012-1mL","price":535.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302112333899,"sku":"S0K0012-2mL","price":920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7fd3e8b476d347b6a496a6ad4641ca34.jpg?v=1788174152"},{"product_id":"cd3-nanobeads-humanruo-s0k0011","title":"CD3 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD3 monoclonal antibody.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD3 Nanobeads are used to magnetically label human CD3⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD3⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD3⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD3⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD3⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD3 is expressed on the surface of all T cells and is associated with the T-cell receptor. In human peripheral blood lymphocytes, 70–80% are CD3+, and in thymocytes, 65–85% are CD3+. The antigenic epitope recognized by CD3 Nanobeads is located on the CD3ε chain.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302114299979,"sku":"S0K0011-1mL","price":285.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302114332747,"sku":"S0K0011-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e0d8a9b1868547838ef9f1a0046ebceb.jpg?v=1788177630"},{"product_id":"cd8-nanobeads-humanruo-s0k0010","title":"CD8 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD8 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD8⁺ cells are magnetically labeled using CD8 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD8⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD8 antigen forms a complex with the T-cell receptor and serves as a co-molecule on CD8+ cytotoxic T cells, assisting the TCR heterodimer in recognizing MHC class I–peptide complexes. The CD8 molecule consists of an α\/β heterodimer or an α\/α homodimer. It is highly expressed on cytotoxic T cells and weakly expressed on some NK cells. CD8 is present on most thymocytes and approximately one-third of peripheral blood T cells. CD8+ cytotoxic T cells play a crucial role in eliminating virus-infected cells and tumor cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":43030516990027,"sku":"S0K0010-1mL","price":300.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302114725963,"sku":"S0K0010-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/be2c1ea3e1e14ee287d90f97830f0c6a.jpg?v=1788177634"},{"product_id":"cd4-nanobeads-humanruo-s0k0013","title":"CD4 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD4 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD4⁺ cells are magnetically labeled using CD4 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD4⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD4⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD4⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD4⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD4 NanoBeads are designed to isolate human cells based on the expression of the CD4 antigen. CD4 is a 55 kDa protein that is highly expressed on helper T cells in the thymus and peripheral blood. In helper T cells, CD4 functions as a co-molecule, assisting the TCR heterodimer in recognizing MHC class II–peptide complexes. CD4 is also expressed on monocytes and dendritic cells, though at lower levels than in CD4+ helper T cells. When bound to certain chemokine receptors, CD4 serves as the primary high-affinity receptor for HIV.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":42302115217483,"sku":"S0K0013-1ml","price":300.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302115250251,"sku":"S0K0013-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6603e7615e5a4b1197d3ed41f7eeecf8.jpg?v=1788177637"},{"product_id":"cd3-nanobeads-kit-mouse-ruo-s0k0015","title":"CD3ε Nanobeads Kit, mouse (RUO)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eBiotinylated anti-mouse CD3ε monoclonal antibody and streptavidin-conjugated nanomagnetic beads, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: Suitable for a total cell count of 5×10⁸, allowing for up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: Suitable for a total cell count of 1×10⁹, allowing for up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eFirst, CD3ε+ cells are labeled with a CD3ε-biotin–conjugated primary antibody. Subsequently, the cells are magnetically labeled using biotin-conjugated nanomagnetic beads. The cell suspension is then placed in a separation column within a magnetic field. The CD3ε+ cells that have been labeled with the magnetic beads remain retained in the column, while the unlabeled cells flow through; thus, CD3ε+ cells are separated from the other cell populations.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eCD3ε is a transmembrane protein with a molecular weight of 20 kDa and serves as a subunit of the T-cell receptor (TCR) complex. Together with the γ and δ subunits of CD3, it plays a crucial role in the assembly and expression of the TCR complex. CD3 is expressed on the surface of thymocytes, mature T lymphocytes, and natural killer T cells (NKT cells). Using the CD3ε nanosphere kit, mouse T lymphocytes and NKT cells can be depleted from various tissues, including the spleen, thymus, and lymph nodes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302138810443,"sku":"S0K0015-1mL","price":335.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302138843211,"sku":"S0K0015-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ffa06f046bae49d681275dde28f550af.jpg?v=1788184921"},{"product_id":"mouse-neutrophil-isolation-kit-column-based-s0k1015","title":"Mouse Neutrophil Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Neutrophil Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate Mouse Neutrophil cells from mouse bone marrow. In this negative selection process, non‑Mouse Neutrophil cells are labeled with biotin‑conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody complexes, while the target Mouse Neutrophil cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled Mouse Neutrophil cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of Mouse Neutrophil cells after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNeutrophils in their native, unmanipulated state are isolated from mouse bone marrow and peripheral blood and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe specific phenotype of mouse neutrophils is CD11b+Ly6G+. They are the most abundant innate immune cells in mouse peripheral blood and bone marrow, serving as the body's first line of defense against bacterial and fungal infections. They play a central role in physiological and pathological processes such as inflammatory responses, immune regulation, tumor microenvironment remodeling, and tissue damage repair. The mouse neutrophil negative-selection kit is developed based on immunomagnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive-selection methods. By specifically depleting non-target cells, it yields unlabeled, native-state, highly purified neutrophils, perfectly preserving their natural activity and physiological functions, and is well suited for various precise cell functional assays, making it the mainstream research tool for isolating mouse neutrophils today.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the sorting buffer; it must be provided separately. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column \u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect mouse bone marrow or peripheral blood, prepare a single-cell suspension, and perform cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eMouse bone marrow and peripheral blood must undergo red blood cell lysis using Red Blood Cell Lysis Buffer (without fixative, 1X) (Cat: S0D9002); it is recommended to count cells after staining with AO\/PI;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1 × 10^7 cells in 100 μL of MagSep Separation Buffer;\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; retain a pre-sorting sample to determine the proportion of mouse neutrophils;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse Neutrophil Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gently pipette up and down 2–3 times to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at 2–8°C for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at 2–8°C for 15 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 minutes, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column selection magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells in 1 mL of MagSep Separation Buffer and load them into the separation column, collecting the target cells in a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer three times, collecting the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: 1. Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column has a maximum capacity of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42917951766603,"sku":"S0K1015-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5f8143674be5432db0535e4c6ec96ae8.jpg?v=1788858141"},{"product_id":"cd19-nanobeads-mouseruo-s0k0024","title":"CD19 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD19 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD19 Nanobeads, mouse are used to magnetically label CD19⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD19⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD19⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD19⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIsolate or deplete CD19+ cells from a single-cell suspension of mouse spleen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouse CD19 is encoded by the Cd19 gene and is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, serving as a classic marker of the B-cell lineage. This molecule begins to be expressed at the pro-B-cell stage in the bone marrow and remains on the surface of the vast majority of mature B cells, while its expression is downregulated in terminally differentiated plasma cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":42941098721355,"sku":"S0K0024-1 mL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":42941098754123,"sku":"S0K0024-2 mL","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b7442e19cfcf48c5b74a7c68b921413f.jpg?v=1788249817"},{"product_id":"streptavidin-nanobeads120-nm-s0k3012","title":"Streptavidin Nanobeads（120 nm）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStreptavidin-conjugated iron oxide nanomagnetic beads, in a PBS buffer system containing BSA and ProClin 300.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCells are stained with biotinylated primary antibodies or ligands, then magnetically labeled using Streptavidin Nanobeads (120 nm). The cell suspension is subsequently transferred to a polystyrene tube and placed within the magnetic field of a sorter. Under the influence of the magnetic field, the magnetically labeled cells adhere to the tube wall, while unlabeled cells remain in the supernatant. After collecting the supernatant, the magnetically retained cells can be eluted.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eStreptavidin Nanobeads (120 nm) are designed to isolate cells based on surface markers labeled with biotinylated antibodies or ligands.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include separation buffer; please prepare it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking Mouse T column-free negative selection as an example\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eTo achieve better sorting results and cell yield, the amount of magnetic beads needs to be optimized based on different antibody concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the mouse spleen, grind it on a 40 μm sterile filter to prepare a single-cell suspension; after red blood cell lysis, proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; set aside a pre-sorting sample to determine the initial Mouse T ratio;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle pipetting 2–3 times can be used to mix thoroughly.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin Nanobeads (120 nm).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 5 μL of Streptavidin Nanobeads (120 nm) to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, you can use a pipette to gently blow the mixture 2–3 times.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn this step, the magnetic beads may settle at the bottom. You can gently tap to mix them after 2.5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the ring-shaped magnetic stand to prevent the magnetic beads from settling excessively.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdjust the total volume to 2.5 mL (using a 5 mL flow cytometry tube).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the ring-shaped magnetic stand so that the magnetic beads adhere to the tube wall.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAllow adsorption for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic rack and pour the sample into a new collection tube to collect it.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The sorted cells can be centrifuged at 400g for 7 minutes for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor 0.1–1 × 10^8 cells (0.1–1 mL), it is recommended to use a 5 mL single-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) with a total adsorption volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":42955271831627,"sku":"S0K3012-1 mL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/55e666026c5a471bba187e03a1805262.jpg?v=1788235216"},{"product_id":"human-nk-cell-isolation-kit-column-based-s0k1009","title":"Human NK Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human NK Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-NK cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the non‑target cells are marked by the antibody‑bead complex, while the target NK cells remain unlabeled. Subsequently, the cell suspension is transferred to a separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells adhere to the column, whereas the unlabeled NK cells elute into a collection tube under gravity. The resulting supernatant constitutes the NK‑cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eHuman NK cells (Natural Killer Cells) are key effector cells of the innate immune system, accounting for 5%–20% of peripheral blood lymphocytes. They can directly kill virus-infected and tumor cells without prior antigen sensitization and secrete large amounts of cytokines to regulate immune responses, making them a critical focus in cancer immunology, anti-infectious immunity, and immunotherapy research. NK cells isolated using an NK cell negative selection kit are suitable for research scenarios where direct surface molecule labeling might interfere with downstream applications; the purified NK cells can be immediately used for flow cytometry, cell culture, or DNA\/RNA extraction, among other downstream assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include separation buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell Processing and Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation medium, or directly thaw cryopreserved PBMCs, and perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample volume; set aside a pre-sorting sample to determine the proportion of human NK cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e1×10^7\u003c\/span\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human NK cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix evenly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 20 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace it in the column-type magnetic separator,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. Cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42955273240651,"sku":"S0K1009-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3ee371b650664854b8522f42e8cf1684.jpg?v=1788188590"},{"product_id":"cd138-nanobeads-humanruo-s0k0016","title":"CD138 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles coupled with an anti-human CD138 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD138⁺ cells are magnetically labeled using CD138 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD138⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD138⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD138⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD138⁺ cells must be further separated through a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD138 NanoBeads are specifically developed for the isolation of plasma cells from human peripheral blood mononuclear cells, bone marrow, or lymphoid tissues. CD138, also known as syndecan-1, is expressed on the surface of both normal and malignant human plasma cells and serves as a universal marker for identifying plasma cells. CD138 is also expressed on the basolateral membrane of endothelial cells, embryonic mesenchymal cells, vascular smooth muscle cells, and nerve cells. In mice, in addition to its expression on plasma cells, CD138 is also found on B-cell precursor cells in the bone marrow.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 ml","offer_id":42955273535563,"sku":"S0K0016-1 ml","price":500.0,"currency_code":"USD","in_stock":true},{"title":"2 ml","offer_id":42955273568331,"sku":"S0K0016-2 ml","price":835.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b90e66208627470fb2f17ddf6b478464.jpg?v=1788188565"},{"product_id":"cd15-nanobeads-humanruo-s0k0025","title":"CD15 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD15 monoclonal antibody.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD15⁺ cells are magnetically labeled using CD15 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD15⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD15⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD15⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEosinophils and neutrophils can be isolated from or depleted from tissue samples, such as human peripheral blood or ovarian cancer tissues.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#404040\"\u003eCD15 Nanobeads, human are designed to positively select or deplete CD15-positive cells from human peripheral blood or tissue single-cell suspensions. The CD15 antigen is expressed on neutrophils, eosinophils, and myeloid lineage monoblast progenitor cells, but it is not expressed on basophils or lymphocytes. Furthermore, this antigen is also expressed in Hodgkin\/Reed-Sternberg cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":42983073906763,"sku":"S0K0025-1 mL","price":450.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":42983073939531,"sku":"S0K0025-2 mL","price":735.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ec308c2d27c8475f85d0b5c80521a285.jpg?v=1788256845"},{"product_id":"cd66b-nanobeads-humanruo-s0k0023","title":"CD66b Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD66b monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD66b Nanobeads are used to magnetically label human CD66b⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD66b⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD66b⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD66b⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIsolate or deplete CD66b+ cells from human whole blood (following red blood cell lysis).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eCD66b is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular weight of 95–100 kDa, also known as CD67, CGM6, and NCA-95. This molecule is expressed on the surface of neutrophils and eosinophils but is not expressed on basophils or lymphocytes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030515581003,"sku":"S0K0023-1 mL","price":450.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":43030515613771,"sku":"S0K0023-2 mL","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/67961ed5aef74661a1368090255c75bb.jpg?v=1788235356"},{"product_id":"streptavidin-nanobeads300-nm-s0k3011","title":"Streptavidin Nanobeads（300 nm）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStreptavidin-conjugated iron oxide nanomagnetic beads, in a PBS buffer system containing BSA and ProClin 300.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCells are stained with biotinylated primary antibodies or ligands, then magnetically labeled using Streptavidin Nanobeads (300 nm). The cell suspension is subsequently transferred to a polystyrene tube and placed within the magnetic field of a sorter. Under the influence of the magnetic field, the magnetically labeled target cells adhere to the tube wall, while the unlabeled non-target cells remain in the supernatant. After collecting the supernatant, the magnetically retained cells can be eluted as the target population.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eStreptavidin Nanobeads (300 nm) are designed to isolate cells based on surface markers labeled with biotinylated antibodies or ligands.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking Mouse T column-free negative selection as an example\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. To achieve better sorting results and cell yield, the amount of magnetic beads needs to be optimized based on different antibody concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. For samples with a positive rate below 15%, it is recommended to try Streptavidin Nanobeads (120 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the mouse spleen, grind it on a 40 μm sterile filter mesh to prepare a single-cell suspension of splenic cells; after red blood cell lysis, proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer;\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; retain a pre-sorting sample for initial Mouse T cell ratio testing.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle pipetting up and down 2–3 times can be used to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin Nanobeads (300 nm).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Streptavidin Nanobeads (300 nm) to the sample.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL per 100 μL.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, the mixture can be gently pipetted up and down 2–3 times to homogenize;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom. You can gently tap the tube to mix them after 2.5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling excessively;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdjust the total volume to 2.5 mL (in a 5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdjust the total volume to 7.5 mL (in a 15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand to allow the magnetic beads to adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic rack (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorption using a multi-well magnetic rack (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic rack and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The sorted cells can be centrifuged at 400g for 7 minutes for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: 1.\u003c\/span\u003e\u003cspan\u003e0.1-1 × 10^8 cells (0.1-1 mL): it is recommended to use a 5 mL single-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) to capture a total volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2-5 × 10^8 cells (2-5 mL): it is recommended to use a 15 mL multi-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) to capture a total volume of 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eDead Cell Removal Set, (RUO)\u003c\/span\u003e\u003cspan\u003e#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030515777611,"sku":"S0K3011-1 mL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/106d3ec175fc4c78974314e45892befc.jpg?v=1788210043"},{"product_id":"human-nave-cd4-t-cell-isolation-kit-column-based-s0k1014","title":"Human Naïve CD4 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Naïve CD4 T Cell Biotin-Antibody Cocktail, PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads, PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection approach to isolate Human Naïve CD4 T cells from human peripheral blood mononuclear cells (PBMCs). In this negative-selection process, non-Human Naïve CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody‑bead complex, while the target Human Naïve CD4 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled Human Naïve CD4 T cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of Human Naïve CD4 T cells after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their unmanipulated, label-free state are isolated from human PBMCs and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe immunophenotype of human naïve CD4 T cells is CD3⁺CD4⁺CD45RA⁺CD45RO⁻. They constitute the core precursor population of helper T cells in the human adaptive immune system, responsible for initial antigen recognition and directed differentiation, and are also a key cell subset for maintaining immune homeostasis, mediating specific responses, and establishing immunological memory. The human naïve CD4 T-cell negative-selection kit is developed based on immuno-magnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive-selection methods. By specifically depleting non-target cells—such as CD8 T cells, NK cells, B cells, monocytes, dendritic cells, granulocytes, and memory T cells—it yields unlabeled, native-state, highly purified naïve CD4 T cells, perfectly preserving their initial quiescent state, surface molecule integrity, and multipotential differentiation capacity. This makes them suitable for various functional assays, including in vitro activation and proliferation, subtype-directed differentiation induction, and cytokine secretion analysis, and they have become the mainstream research tool for isolating human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please provide it separately. The corresponding Staret product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eFor example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePeripheral blood mononuclear cells (PBMCs) are isolated from peripheral blood by density-gradient centrifugation using lymphocyte separation medium, or cryopreserved PBMCs are directly thawed and resuspended, followed by cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; PBMCs can be pelleted by centrifugation at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; a pre-sorting sample is retained for assessing the proportion of human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Human Naïve CD4 T Cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIf you want a higher cell yield, after antibody incubation, add twice the volume of MagSep Separation Buffer, centrifuge at 400 g for 7 min, then carefully discard the supernatant. Resuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells, and proceed to the next steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 8 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 8 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to wash the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: This step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: 1. Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can label a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column can label a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030515810379,"sku":"S0K1014-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e4b99aedf25946b9b3973f91ebefd2bc.png?v=1788210041"},{"product_id":"human-b-cell-isolation-kit-column-based-s0k1013","title":"Human B Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human B Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate B cells from human peripheral blood mononuclear cells (PBMCs). In this negative-selection process, non-B cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells become bound to the magnetic beads via the antibody‑antigen complex, while the target B cells remain unlabeled. The cell suspension is subsequently loaded onto a separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled B cells elute by gravity into a collection tube. The resulting supernatant constitutes the B-cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eHuman B lymphocytes are the core effectors of adaptive humoral immune responses, performing critical functions such as antigen recognition, processing and presentation, antibody secretion, and maintenance of immunological memory. They play an irreplaceable role in defending against extracellular pathogen infections and regulating the magnitude of immune responses. The CD19 molecule is the most representative lineage-specific marker on the B-cell surface; it belongs to the immunoglobulin superfamily and is stably expressed from the early pre-B cell stage through to mature plasma cells. CD19 collaborates with the B-cell receptor to regulate B-cell activation, proliferation, and differentiation, serving as a crucial regulatory molecule for B-cell development and functional maintenance. Consequently, it is widely used as a specific surface antigen of human B cells. In immunological research, obtaining B cells that are highly pure, highly active, and have intact, native surface molecules is an essential prerequisite for ensuring the reliability of experimental results. This negative-selection kit is based on the principle of negative sorting, employing a biotin‑labeled antibody panel to specifically label non‑B cells. The identified targets include various interfering cell populations, such as T cells, NK cells, monocytes, macrophages, platelets, and erythroid cells. Streptavidin‑coated nanomagnetic beads are then used to efficiently remove non‑target cells. Throughout the entire sorting process, no antibodies bind to any antigens on the B‑cell surface, thereby maximizing the preservation of the cells' original morphology, the integrity of their surface markers, and their biological functional activity, providing high‑quality cellular material for downstream applications such as gene expression analysis, in vitro culture and expansion, and functional characterization.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; it must be provided separately. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample volume; set aside a pre-sorting sample to determine the proportion of human B cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human B cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace it in the column selection magnet,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eadd 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes: 1.\u003c\/span\u003e\u003cspan\u003ePay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ethe L Separation Column's maximum labeled cell count is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003eDead Cell Removal Set, (RUO)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e#S0K0009\u003c\/span\u003e\u003cspan\u003e);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030515974219,"sku":"S0K1013-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/56a866ae170a41b38b2486e016305ce3.png?v=1788210027"},{"product_id":"human-pan-monocyte-cell-isolation-kit-column-based-s0k1012","title":"Human Pan Monocyte Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Pan Monocyte Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.5 mL Human FcR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-Pan monocytes (CD14+CD16+) are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the unwanted cells are marked by the antibody–bead complex, while the target Pan monocytes (CD14+CD16+) remain unlabeled. The cell suspension is subsequently transferred to a separation column; under the influence of a magnetic field, the magnetically labeled non-target cells adhere to the column, whereas the unlabeled Pan monocytes (CD14+CD16+) elute into a collection tube under gravity. The resulting supernatant represents the enriched fraction of Pan monocytes (CD14+CD16+) after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eMonocytes are an important myeloid cell subset of the innate immune system, capable of differentiating into macrophages and dendritic cells. They participate in physiological and pathological processes such as inflammatory responses, antigen presentation, immune regulation, and tissue repair, making them a commonly used research subject for investigating immunological mechanisms, conducting cellular function experiments, and establishing inflammation models. Based on differences in the expression of CD14 and CD16 molecules on their surface, human monocytes can be classified into classical, intermediate, and non-classical subpopulations. CD14+CD16− monocytes constitute a key functional subtype, extensively involved in inflammatory responses, antigen presentation, immune regulation, and pathogen clearance, and thus hold significant research value in studies related to infection, autoimmune diseases, and tumor immunity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThis reagent employs immunomagnetic bead-based cell sorting to specifically remove contaminating cells such as lymphocytes, granulocytes, and erythrocytes, thereby efficiently enriching human CD14+CD16+ positive pan‑monocytes. The sorting process is gentle, avoiding cell activation and preserving surface markers, thus fully maintaining the cells' native biological characteristics and physiological activity. The isolated cells exhibit high purity and stable viability, making them suitable for applications including cell subtype identification, polarization induction, cytokine secretion assays, in vitro functional validation, and investigations into disease mechanisms. They are well‑suited for basic immunological research and preclinical experimental settings.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; you will need to provide it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking the L Separation Column as an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"8\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation medium, or directly thaw cryopreserved PBMC cells, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to test the proportion of Human Pan Monocyte cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 5 μL of Human FcR to the sample and mix gently;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Human Pan Monocyte cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle pipetting to mix 2–3 times is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells. After centrifuging at 400g for 7 minutes, discard the supernatant and resuspend the cells to the original volume.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to wash the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column has a maximum capacity of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516006987,"sku":"S0K1012-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/65ca4b823fa14664b1b6a73c2efc62a5.jpg?v=1788210025"},{"product_id":"human-cd8-t-cell-isolation-kit-column-based-s0k1011","title":"Human CD8 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human CD8 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-CD8 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the non‑target cells are marked by the antibody‑bead complex, while the target CD8 T cells remain unlabeled. Subsequently, the cell suspension is loaded onto a separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled CD8 T cells elute into a collection tube under gravity. The resulting supernatant constitutes the CD8 T cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman CD8 T lymphocytes are the core effector cells of adaptive cellular immunity in the human body, playing a crucial role in host anti-infection immunity, tumor immune surveillance, and immune homeostasis regulation. Due to their precise antigen specificity, potent cytotoxic function, and immunological memory characteristics, human CD8 T cells are central research subjects in fields such as immunological mechanism studies, anti-infection vaccine development, tumor immunotherapy (e.g., CAR-T cell therapy), and immune function assessment, possessing significant scientific research and clinical application value. This kit employs a column-based negative selection approach: non-CD8 T cells are labeled with biotin-conjugated antibodies, and magnetic bead sorting is used to remove non-target cells, thereby enriching highly pure, high‑activity CD8 T cells. This method avoids the potential impact of direct labeling of CD8 T cells on cellular function and is suitable for subsequent experimental studies, including cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; you need to prepare it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\n\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample volume; set aside a pre-sorting sample to measure the proportion of human CD8 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e1×10^7\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human CD8 T cell Biotin Antibody Cocktail to the sample and gently mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column-based magnetic separator, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516039755,"sku":"S0K1011-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/af31b4675238440196faae33e06abc7e.jpg?v=1788210022"},{"product_id":"mouse-pan-b-cell-isolation-kit-column-based-s0k1010","title":"Mouse Pan B Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Pan B Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate Pan B cells from mouse spleens. In this negative selection process, non-B cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody‑antigen complex, while the target B cells remain unlabeled. The cell suspension is subsequently loaded onto a separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled B cells elute by gravity into a collection tube. The resulting supernatant represents the B‑cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHigh-purity mouse pan-B cells, including conventional B-2 B cells, B-1 B cells, and plasma cells, can be isolated from splenic single-cell suspensions and used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouse Pan B cells (pan-B cells) refer to the entire population of B cells in mice, encompassing all lineages, developmental stages, and functional subtypes. Pan B cells include all B-lineage cells, from early bone marrow progenitor B cells and transitional B cells to peripheral mature follicular B cells (FO B), marginal zone B cells (MZ B), B1 innate-like B cells, memory B cells, and even plasma cell precursors. Their defining characteristic is the expression of broad B-lineage hallmark molecules; the classic markers for mouse Pan B cells are CD19 and B220 (CD45R), with CD19 serving as a lineage-specific, invariant marker that remains expressed throughout development and activation. In immunological research, obtaining highly pure, highly active Pan B cells is essential for conducting functional analyses and signaling pathway studies. Unlike conventional mouse B-cell isolation kits, Pan B-cell isolation fully preserves CD43-positive B-cell subsets, including activated B cells, plasma cells, and CD5-positive B-1a cells, thereby comprehensively covering B-cell populations across different developmental stages and functional phenotypes, preventing the loss of critical B-cell subsets and more accurately reflecting the true composition of B cells in vivo. This negative-selection kit operates on the principle of negative sorting, using a biotin-labeled antibody cocktail (excluding anti-CD19 antibodies) to specifically label non-B cells (such as T cells, NK cells, monocytes, and red blood cells), followed by efficient removal of non-target cells via streptavidin-coated magnetic nanoparticles. Throughout the process, no antigens on the B-cell surface are contacted, maximizing the preservation of cellular morphology, surface marker integrity, and physiological functional activity, thus providing high-quality cell material for downstream experiments.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px;font-weight: bold\"\u003eNote\u003c\/span\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003eThis kit does not include sorting buffer; please provide it separately. The corresponding Stater product number is:\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\"\u003eS0D3018\u003c\/a\u003e.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eFor example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThe spleen was harvested from mice, passed through a 40-μm sterile filter for homogenization to prepare a single-cell suspension, and subjected to red blood cell lysis prior to cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eUse 100 μL.\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eResuspend to 1 × 10⁷\u003c\/span\u003e\u003cspan\u003ecell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; retain a pre-sorting sample for determining the initial B-cell proportion.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 × 10⁷\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Mouse Pan B cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, the mixture can be gently pipetted 2-3 times to ensure uniformity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix the Streptavidin Beads thoroughly, then add 10 μL of Streptavidin Beads to the sample.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the L Separation Column into the column separation magnet and add 3 mL.\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eto rinse the separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell separation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, use\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer.\u003c\/span\u003e\u003cspan\u003eComplete; cells that drip down due to gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003e, and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unmarked target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes: 1.\u003c\/span\u003e\u003cspan\u003ePay attention to the maximum capacity of the separation column; the S Separation Column can handle a maximum of 1×10⁷ labeled cells.\u003c\/span\u003e\u003cspan\u003eThe L Separation Column can handle a maximum of 1×10⁸ labeled cells.\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the viability of the sample is low, it is recommended to use a dead cell removal kit to remove dead cells (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eDead Cell Removal Set, (RUO)\u003c\/span\u003e\u003cspan\u003e#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516072523,"sku":"S0K1010-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9b276debfccb447c8766878c7fef54ff_9fca9ecd-113d-4133-aae3-3ec637b8aefa.jpg?v=1789880439"},{"product_id":"cd11c-nanobeads-kit-mouse-ruo-s0k0022","title":"CD11c Nanobeads Kit, mouse (RUO)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnetic beads conjugated with an anti-mouse CD11c monoclonal antibody, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003enine\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eTotal cell count: up to 100 sorting runs can be performed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, enabling up to 200 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD11c⁺ cells are magnetically labeled using CD11c Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD11c⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after depletion of CD11c⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD11c⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIsolate or deplete CD11c+ cells from a single-cell suspension of mouse spleen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eCD11c Nanobeads are specifically designed for the isolation of CD11c-positive cells (such as dendritic cells, DCs) from single-cell suspensions derived from murine lymphoid and non-lymphoid tissues. Unlike human cells, all known subsets of dendritic cells in mice express CD11c, and certain tissue-resident macrophage subsets also carry this marker. By using these CD11c magnetic beads, you can replace the traditionally laborious and complex steps of dendritic cell isolation with a simple and rapid positive-selection protocol.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030516105291,"sku":"S0K0022-1 mL","price":500.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":43030516138059,"sku":"S0K0022-2 mL","price":833.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a04d746cd85a41c2a49e366ebfcfee88.png?v=1788210019"},{"product_id":"cd45r-nanobeads-mouseruo-s0k0021","title":"CD45R Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads conjugated with an anti-mouse CD45R monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eMethod\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD45R Nanobeads, mouse\u003c\/span\u003e\u003cspan\u003eMagnetic labeling of CD45R-positive cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45R cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD45R cells. After removing the separation column from the magnetic field, the magnetically captured CD45R-positive cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD45R cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eCD45R Nanobeads, mouse\u003c\/span\u003e\u003cspan\u003eDesigned for positive selection or depletion of B cells from lymphoid tissues, peripheral blood, or bone marrow. The CD45R antigen is expressed throughout the entire development of B lymphocytes, starting from the early pro-B cell stage, and is downregulated upon terminal differentiation into plasma cells. CD45R is commonly used as a pan-B cell marker. It is also expressed on a small subset of dendritic cells (plasmacytoid dendritic cells).\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030516170827,"sku":"S0K0021-1 mL","price":500.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":43030516203595,"sku":"S0K0021-2 mL","price":833.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/694e65c3bd8b4f1a8b2aca8417869f82.jpg?v=1788210017"},{"product_id":"t-cell-activation-nanobeadshumanruo-s0k0020","title":"T Cell Activation Nanobeads，Human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanobeads with surface-coupled anti-human CD3 and anti-human CD28 specific antibodies;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e1 mL Human T Cell Activation Nanobeads: can activate 2 × 10⁸ T cells;\u003cbr\u003e0.5 mL Human T Cell Activation Nanobeads: can activate 1 × 10⁸ T cells.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;1EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePrecautions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAll operations throughout the entire process must be performed in a sterile environment;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eFor initial activation, ensure that the reagent continuously interacts with the cells for 2–3 days; removing the reagent too early will result in insufficient T-cell activation and reduced proliferative capacity;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eT cells can undergo viral transduction experiments two days after activation; the optimal transduction titer and timing should be determined independently through experimentation;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eResidual EDTA in the cell system significantly inhibits T-cell activation stimulation; it must be thoroughly removed before starting the experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2. Sample Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eCD4 Nanobeads, human (Cat. No. S0K0013), CD8 Nanobeads, human (Cat. No. S0K0005), and CD3 Nanobeads, human (Cat. No. S0K0011) can be used to isolate and enrich T cells from PBMCs. Refer to the corresponding product manuals for specific procedures.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003ePeripheral blood samples from humans need to be separated by density gradient centrifugation to obtain PBMCs, which are then used for subsequent T-cell activation experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e3. Cell Seeding Density and Reagent Usage Standards\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eThis experimental protocol optimally recommends using a working dilution of 1:200; the best seeding density for sorted T cells is 1×10⁶ cells\/cm²; the optimal seeding density for PBMCs is 2×10⁶ cells\/cm².\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eOptimal Culture Medium Formulation Table for Each Type of Culture Plate\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eCulture Plate Model\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eGrowth Surface Area\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTotal Culture Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eT-Cell Inoculum Quantity\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eNanobeads Addition Amount\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eCell Culture Medium Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e96-well plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.31 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.3 × 10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e199 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e48-well plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 × 10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e995 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e24-well plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 × 10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1990 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e12-well plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e20 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3980 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6-well plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4975 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNotes: When the cell count is less than 10⁶, still add reagents in fixed volumes as per the table; when scaling up the cell culture, proportionally increase the total volumes of reagents and culture medium.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e4. Standard activation procedure for a 48-well plate\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eUse AO\/PI to count cells and determine the required cell amount;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eResuspend the cells in 995 μL of complete T-cell culture medium;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAdd 5 μL\u003c\/span\u003e\u003cspan\u003eT Cell Activation Nanobeads\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eand mix gently and thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePlace in a 37℃, 5% CO₂ incubator and let it sit for 48 hours;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAfter the initial activation, perform centrifugation and media exchange:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e① Centrifuge at 300×g for 10 min;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e② Slowly and completely aspirate the supernatant from the upper layer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e③ Add fresh T cell complete culture medium at a concentration of 1×10⁶\/ml;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e④ Incubate in a 37℃, 5% CO₂ incubator to continue culturing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e5. Cell expansion and culture procedure\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eEvery 2 days, equally divide the cell suspension into new wells or flasks, and replenish with fresh culture medium;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eMaintain standard culture conditions at 37℃ and 5% CO₂;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eBy day 12 of culture, the cells have expanded to sufficient numbers and can be used for various downstream experiments;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eDepending on the cells' growth status and experimental design requirements, the expansion culture period can be extended up to 16 days at most;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eIf long-term expansion culture is needed, T Cell Activation Nanobeads can be added again after day 12 of culture.\u003c\/span\u003e\u003cspan\u003eT Cell Activation Nanobeads\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePerform secondary activation stimulation.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"0.5 mL","offer_id":43030516236363,"sku":"S0K0020-0.5 mL","price":300.0,"currency_code":"USD","in_stock":true},{"title":"1 mL","offer_id":43030516269131,"sku":"S0K0020-1 mL","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a3b06f5569264f0ea9099f1ed448d80d.jpg?v=1788210016"},{"product_id":"cd56-nanobeads-humanruo-s0k0019","title":"CD56 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD56 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD56⁺ cells are magnetically labeled using CD56 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD56⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD56⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD56⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eCD56 Nanobeads, human\u003c\/span\u003e\u003cspan\u003eIt is designed to isolate human cells based on the expression of the CD56 antigen. CD56 is predominantly expressed on the surface of all human NK cells, and its density on the cell membrane increases upon activation. This antigen is also present in a distinct population of CD3-positive cells.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eOn T cells (mediating MHC‑unrestricted cytotoxicity), as well as on myoblasts, certain neural tissues, and tumors.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030516301899,"sku":"S0K0019-1 mL","price":416.67,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":43030516334667,"sku":"S0K0019-2 mL","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5d0aab332eda45429e52799ea19ec8ad.jpg?v=1788210013"},{"product_id":"anti-biotin-nanobeads-s0k0018","title":"Anti-biotin Nanobeads","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with mouse anti-biotin monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStain the cells with biotinylated primary antibodies or ligands. Subsequently, magnetically label the cells using Anti-biotin Nanobeads. Then, load the cell suspension onto a separation column placed within the separator's magnetic field. The magnetically labeled cells are retained inside the column, while the unlabeled cells pass through. After removing the column from the magnetic field, the cells that were retained magnetically can be eluted as the positively selected cell fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eAnti-biotin Nanobead\u003c\/span\u003e\u003cspan\u003eis designed for the indirect magnetic labeling and separation of cells or other biological materials that have been labeled with biotinylated primary antibodies, ligands, or biotinylated antibody mixtures. The biotinylated molecules are recognized by anti-biotin monoclonal antibodies conjugated to the magnetic beads.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":43030516367435,"sku":"S0K0018-1 mL","price":416.67,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":43030516400203,"sku":"S0K0018-2 mL","price":666.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/295000b135004245b70cfad94284dea3_ee84f36b-c7fa-44fd-a1b6-e58cabb65085.jpg?v=1789462824"},{"product_id":"cd49b-nanobeads-mouseruo-s0k0017","title":"CD49b Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD49b monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, allowing up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, allowing up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eAdopt\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD49b Nanobeads, mouse\u003c\/span\u003e\u003cspan\u003efor magnetic labeling of CD49b cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD49b cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD49b cells. After removing the separation column from the magnetic field, the magnetically captured CD49 cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD49b cells should be further separated using a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD49b Nanobeads, mouse are designed to isolate mouse NK cells from single-cell suspensions of lymphoid and non-lymphoid tissues, peripheral blood, or body fluids. CD49b is expressed on NK cells and a small subset of T cells (CD4⁺CD3⁺TCRαβ⁺). Compared with other NK cell markers such as NK1.1, CD49b exhibits lower strain specificity in mice and is expressed in most commonly used inbred mouse strains.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":43030516432971,"sku":"S0K0017-1mL","price":500.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":43030516465739,"sku":"S0K0017-2mL","price":833.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/30db32e0f8b14cf88edf765e92dda4dd.jpg?v=1788199370"},{"product_id":"mouse-t-cell-isolation-kit-column-based-s0k1008","title":"Mouse T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.5mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate T cells from mouse spleens. In this negative selection process, non-T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody‑antigen complex, while the target T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled T cells elute by gravity into the collection tube. The resulting supernatant represents the T‑cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse T lymphocytes are the most central effector cell population in the murine adaptive immune system, serving as a key force in defending the body against pathogen invasion, eliminating abnormal cells, and maintaining immune homeostasis. The CD3 molecule is the most characteristic transmembrane glycoprotein complex on the surface of mouse T cells, composed of four polypeptide chains—γ, δ, ε, and ζ—joined by noncovalent bonds. Together with the T-cell receptor (TCR), they form the TCR–CD3 complex. Although the CD3 molecule does not directly recognize antigens, its primary function is to transmit the activation signals generated by TCR–antigen interactions into the cell, thereby initiating T-cell activation, proliferation, and differentiation. It is an essential prerequisite for T-cell maturation, activation, and functional execution; consequently, the CD3 molecule is often used as a specific surface marker for identifying T cells. In scientific research, obtaining highly pure, high‑activity mouse T cells is a prerequisite for conducting T‑cell‑related studies. This negative selection kit employs the principle of negative sorting: biotin‑conjugated antibodies specifically label non‑CD3⁺ T cells (such as B cells, macrophages, and granulocytes), which are then bound and removed using streptavidin‑coated nanomagnetic beads. Throughout the process, there is no binding to T‑cell surface antigens, allowing for maximum preservation of their native morphology, integrity of surface markers, and functional activity, thus providing reliable cellular material for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include separation buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell Processing and Labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake the mouse spleen, grind it on a 40 μm sterile filter to prepare a single-cell suspension of the spleen, perform red blood cell lysis, and then count the cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to determine the initial T-cell ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix it evenly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 5 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003ein the column separation magnet, then add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes: 1.\u003c\/span\u003e\u003cspan\u003ePay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ethe L Separation Column's maximum labeled cell count is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003eDead Cell Removal Set, (RUO)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e#S0K0009\u003c\/span\u003e\u003cspan\u003e);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516760651,"sku":"S0K1008-100T","price":766.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a16fe67efe544cf187dbd5b7eae15cfd.jpg?v=1788184939"},{"product_id":"mouse-cd8-t-cell-isolation-kit-column-based-s0k1007","title":"Mouse CD8 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse CD8 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate CD8 T cells from mouse spleens. In this negative selection process, non-CD8 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells become bound to the beads via the antibody‑bead complex, while the target CD8 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled CD8 T cells elute by gravity into the collection tube. The resulting supernatant represents the CD8 T cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse CD8⁺ T cells are the core cytotoxic effector cells of the murine adaptive immune system. They primarily mature in the thymus and are widely distributed throughout peripheral immune organs and tissues. These cells serve as a critical defense against intracellular pathogens (such as viruses and intracellular bacteria) and the clearance of abnormal cells (such as tumor cells). They also participate in the formation of immunological memory and the regulation of immune responses, making them of great significance in immunology research and experimental applications. After isolation, the cells remain unmarked by surface antibodies and retain their native state, enabling subsequent in vitro activation, differentiation, and functional studies.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include separation buffer; you need to prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake the mouse spleen, grind it on a 40 μm sterile filter mesh to prepare a single-cell suspension of the spleen, perform red blood cell lysis, and then count the cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to determine the initial CD8 ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse CD8 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, the mixture can be gently pipetted 2–3 times to homogenize;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 min, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: 1. Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in the S Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in the L Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the viability of the sample is low, it is recommended to use the Dead Cell Removal Kit (Dead Cell Removal Set, (RUO)#S0K0009) to remove dead cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516793419,"sku":"S0K1007-100T","price":766.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bb22e059113740ac8a149cb87d633647.jpg?v=1788184934"},{"product_id":"mouse-cd4-t-cell-isolation-kit-column-based-s0k1005","title":"Mouse CD4 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.75mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate CD4 T cells from mouse spleens. In this negative selection process, non-CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells become bound to the magnetic beads via the antibody‑bead complex, while the target CD4 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled CD4 T cells elute by gravity into the collection tube. The resulting supernatant represents the CD4 T cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse CD4 T lymphocytes are a core functional cell subset of the murine adaptive immune system. They originate from hematopoietic stem cells in the bone marrow, complete their development and maturation in the thymus, and then migrate to secondary lymphoid organs such as the spleen and lymph nodes. These cells specifically express the CD4 molecule on their surface and can recognize antigen peptides presented by antigen‑presenting cells (APCs) in complex with MHC class II molecules via the T‑cell receptor (TCR), thereby becoming activated and exerting immunoregulatory functions; they are often referred to as the \"command cells\" of the immune system. In scientific research, obtaining highly purified, high‑activity mouse CD4 T cells is essential for conducting related studies. This negative selection kit employs a negative sorting strategy: biotin‑conjugated antibodies label non‑CD4⁺ T cells, which are then bound by streptavidin‑coated magnetic nanoparticles and removed, keeping CD4 T cells untouched throughout the process. This approach maximizes the preservation of their native morphology and functional activity, providing reliable cellular material for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the sorting buffer; you need to prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake the mouse spleen, grind it on a 40 μm sterile filter mesh to prepare a single-cell suspension of the spleen, perform red blood cell lysis, and then count the cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to measure the initial CD4 ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse CD4 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 min, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 7.5 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7.5 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes: 1.\u003c\/span\u003e\u003cspan\u003ePay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells for the S Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells for the L Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the viability of the sample is low, it is recommended to use a dead cell removal kit to eliminate dead cells (\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eDead Cell Removal Set, (RUO)#S0K0009\u003c\/span\u003e\u003cspan\u003e);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516826187,"sku":"S0K1005-100T","price":766.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1b22da5c7aac428d8735f34a9790ec16.jpg?v=1788184935"},{"product_id":"human-t-cell-isolation-kit-column-based-s0k1004","title":"Human T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-coated nanomagnetic beads. As a result, the non-target cells are marked by the antibody‑bead complex, while the target T cells remain unlabeled. Subsequently, the cell suspension is transferred to a separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained in the column, whereas the unlabeled T cells elute into a collection tube under gravity. The resulting supernatant represents the T-cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman T cells are key immune cells in the immune system, playing a central role in immune responses, immune regulation, and defense against infection and tumors. This kit employs a column-based negative selection approach: non-T cells are labeled with biotin-conjugated antibodies, and unwanted cells are removed using magnetic bead separation, thereby enriching highly pure, high‑activity T cells. This method avoids the potential impact of direct labeling on T cell function and is suitable for downstream experimental studies such as cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; you need to prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to determine the proportion of human T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10^7\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace it in the column-type magnetic separator,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, elute the unlabeled target cells from the separation column to increase the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in an S Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in an L Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516858955,"sku":"S0K1004-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6965634395284954b1e21662bc0c2c78.jpg?v=1788184931"},{"product_id":"human-cd4-t-cell-isolation-kit-column-based-s0k1003","title":"Human CD4 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the non‑target cells become bound to the magnetic beads via the antibody complexes, while the target CD4 T cells remain unlabeled. Subsequently, the cell suspension is loaded onto a separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled CD4 T cells elute by gravity into a collection tube. The resulting supernatant represents the CD4 T cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman CD4 T cells are key immune cells in the immune system, playing a central role in immune responses, immune regulation, and defense against infection and tumors. This kit employs a column-based negative selection approach: non-CD4 T cells are labeled with biotin-conjugated antibodies, and unwanted cells are removed using magnetic bead separation, thereby enriching highly pure, high‑activity CD4 T cells. This method avoids the potential impact of direct labeling on CD4 T cell function and is suitable for downstream experimental studies such as cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\n\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell Processing and Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation medium, or directly thaw cryopreserved PBMCs, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample volume; set aside a pre-sorting sample to determine the proportion of human CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e1×10^7\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human CD4 T cell Biotin Antibody Cocktail to the sample and gently mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column-based magnetic separator, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume; the cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in an S Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe maximum number of labeled cells in an L Separation Column is\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516891723,"sku":"S0K1003-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/23181d18eccb446bb45c0abe4db59677.jpg?v=1788184929"},{"product_id":"mouse-nave-cd8-t-cell-isolation-kit-column-based-s0k1002","title":"Mouse Naïve CD8 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Naïve CD8 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection approach to isolate naïve CD8 T cells from mouse spleens. In this negative-selection protocol, non-naïve CD8 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells become bound to the magnetic beads via the antibody complexes, while the target naïve CD8 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled naïve CD8 T cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of naïve CD8 T cells after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse naïve CD8 T cells are cytotoxic T cells that have matured in the thymus but have not yet encountered specific antigens; they remain in a quiescent state. These cells primarily reside in peripheral lymph nodes and the spleen, where they rely on IL-7 signaling to maintain homeostatic survival. Upon activation by exogenous antigens and costimulatory signals, they undergo extensive clonal expansion and differentiate into effector CD8⁺ T cells (CTLs) with direct cytotoxic activity and long-lived memory CD8⁺ T cells. They serve as the pivotal initiating cells of the organism's adaptive immune responses against viruses and tumors. Using negative magnetic bead sorting to isolate and purify naïve CD8⁺ T cells from mouse splenic cell suspensions yields a purity exceeding 90%. The sorted cells bear no surface antibody labeling and retain their natural quiescent state, making them suitable for subsequent in vitro activation, differentiation, and functional studies.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include separation buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake the mouse spleen, grind it on a 40 μm sterile filter to prepare a single-cell suspension of the spleen, perform red blood cell lysis, and then count the cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to the sample volume; set aside a pre-sorting sample to determine the initial naïve CD8 ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse naïve CD8 T cell Biotin Antibody Cocktail to the sample and gently mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle pipetting to mix 2–3 times is recommended;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 min, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 20 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to wash the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes\u003c\/span\u003e\u003cspan\u003e: 1. Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can label a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column has a maximum labeling capacity of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516924491,"sku":"S0K1002-100T","price":833.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0abbd93463af4ce197f9822eb23d9614.jpg?v=1788184931"},{"product_id":"mouse-nave-cd4-t-cell-isolation-kit-column-based-s0k1001","title":"Mouse Naïve CD4 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Naïve CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Mouse CD44 Nanobeads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate naïve CD4 T cells from mouse spleens. In this negative selection process, non‑naïve CD4 T cells are labeled with biotin‑conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the non‑target cells become bound to the magnetic beads via the antibody complexes, while the target naïve CD4 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled naïve CD4 T cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of naïve CD4 T cells after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their unmanipulated, native state are sorted from a single-cell suspension of fresh mouse spleen and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eMouse naive CD4⁺ T cells (initial CD4-positive T lymphocytes) are mature CD4⁺ T cells that have not yet encountered specific antigens and have not undergone activation, proliferation, or differentiation. By flow cytometry, their canonical phenotype is defined as CD4⁺CD44⁻CD62L⁺. CD62L (L-selectin) is a key receptor for lymph node homing, highly expressed on quiescent naive T cells; upon cell activation or exposure to mechanical or thermal stress, it is rapidly cleaved and shed by the ADAM17 metalloprotease. CD44 is an adhesion molecule characteristic of activated and memory T cells, and is barely expressed on resting naive CD4⁺ T cells. Based on these features, naive T cells (CD44⁻CD62L⁺), central memory T cells (CD44⁺CD62L⁺), and effector memory T cells (CD44⁺CD62L⁻) can be precisely distinguished. Cell purity is a critical prerequisite for functional assays involving naive CD4⁺ T cells. This kit employs immunomagnetic bead-based negative selection: biotinylated antibodies specifically label non-target cells, including B cells, CD8⁺ T cells, myeloid cells, and activated\/memory T cells, which are then selectively removed using streptavidin-conjugated magnetic beads. Throughout the process, the target naive CD4⁺ T cells remain unlabeled and untouched, thereby maximizing preservation of their quiescent phenotype, viability, and differentiation potential. This approach efficiently yields high-purity, highly active, unactivated \"untouched\" naive CD4⁺ T cells, suitable for various experimental applications such as in vitro polarization, cell activation assays, and studies of immune mechanisms.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px;font-weight: bold\"\u003eNote\u003c\/span\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003eThis kit does not include sorting buffer; please provide it separately. The corresponding Stater product number is:\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\"\u003eS0D3018\u003c\/a\u003e.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eFor example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"8\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThe spleen was harvested from mice, passed through a 40-μm sterile filter to prepare a single-cell suspension, and subjected to red blood cell lysis using Red Blood Cell Lysis Buffer (without fixative, 1X) (Cat: S0D9002) prior to cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThroughout the grinding, filtration, and washing steps, use pre-cooled reagents and handle samples gently; avoid vigorous vortexing. It is recommended to count cells after staining with AO\/PI. For splenic cells, centrifuge at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eUsing 100 μL of pre-cooled\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eResuspend to 1 × 10⁷\u003c\/span\u003e\u003cspan\u003ecell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; retain a pre-sorting sample for testing the naïve CD4 percentage.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 × 10⁷\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Mouse Naive CD4 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, the mixture can be gently mixed by pipetting up and down 2–3 times.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies and cells in the cocktail at 2–8°C for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 10 min.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 times the cell volume of pre-cooled\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eWash the cells, centrifuge at 400g for 7 minutes, discard the supernatant, and resuspend to the original volume.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix the Streptavidin Beads thoroughly, then add 10 μL of Streptavidin Beads to the sample.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix the Mouse CD44 Nanobeads well, then add 10 μL of Mouse CD44 Nanobeads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at 2–8°C for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column-based magnetic separator, install the L Separation Column and add 3 mL\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eto rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 1 mL; if insufficient, use\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eto make up the volume; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003ethree times to collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes and sorts the unlabeled target cells, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNotes:\u003c\/span\u003e\u003cspan\u003e1. Pay attention to the maximum capacity of the separation column: the S Separation Column can handle a maximum of 1×10⁷ labeled cells;\u003c\/span\u003e\u003cspan\u003ethe L Separation Column can handle a maximum of 1×10⁸ labeled cells.\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. Throughout the entire process, carefully control the temperature. Keep the sorting buffer pre-cooled at all times, and proceed with subsequent experiments immediately after sorting. For flow cytometry, perform staining in the dark at 2–8°C to avoid prolonged exposure at room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516957259,"sku":"S0K1001-100T","price":833.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/98d17fc3af7c42059a272ad843cca162_4641bcec-7e16-4a51-9e4a-48e681a0002e.jpg?v=1789880441"},{"product_id":"streptavidin-nanobeads-s0k0008","title":"Streptavidin Nanobeads","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStreptavidin-conjugated dextran-coated iron oxide magnetic nanoparticles\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.5 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStain the cells with biotinylated primary antibodies or ligands. Subsequently, magnetically label the cells using Streptavidin Nanobeads. Then, load the cell suspension onto a separation column placed within the separator's magnetic field. The magnetically labeled cells are retained inside the column, while the unlabeled cells pass through it. After removing the column from the magnetic field, the magnetically retained cells can be eluted as the positively selected cell fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eStreptavidin Nanobeads\u003c\/span\u003e\u003cspan style=\"color:#0F1115\"\u003eDesigned to isolate cells based on surface markers labeled with biotinylated antibodies or ligands.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"0.5ml","offer_id":43030517252171,"sku":"S0K0008-0.5ml","price":183.33,"currency_code":"USD","in_stock":true},{"title":"1mL","offer_id":43030517284939,"sku":"S0K0008-1mL","price":283.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a10d3270546f4bd28b4adb8d9dea86a2.png?v=1788293001"},{"product_id":"cd11b-nanobeads-human-and-mouseruo-s0k0007","title":"CD11b NanoBeads, human and mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnetic beads conjugated with anti-human and anti-mouse CD11b monoclonal antibodies, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e1. For human whole blood or mouse bone marrow cells: Add 20 μL of nanobeads per 10⁷ cells, allowing for up to 100 separations. 2. For human PBMCs: Add 6 μL of nanobeads per 10⁷ cells, allowing for up to 333 separations. 3. For mouse splenocytes: Add 2 μL of nanobeads per 10⁷ cells, allowing for up to 1,000 separations.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eAdopt\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD11b NanoBeads, human and mouse\u003c\/span\u003e\u003cspan\u003eMagnetic labeling of CD11b⁺ cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD11b⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD11b⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD11b⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD11b NanoBeads, human and mouse are designed to isolate human and murine cells based on the expression of the CD11b antigen. In humans, CD11b is highly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes. In mice, the CD11b antigen is expressed on monocytes\/macrophages, with lower levels observed on granulocytes, NK cells, CD5+ B1 cells, and certain subsets of dendritic cells. This CD11b antibody (Mac-1 α chain; integrin αM chain) binds to the 170 kDa αM subunit within the CD11b\/CD18 heterodimer (Mac-1, αMβ2 integrin). It functions as a receptor for complement components (C3bi), fibrinogen, or coagulation factor X.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517383243,"sku":"S0K0007-1ml","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":43030517416011,"sku":"S0K0007-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/090e7d07900c4e018316021794bf0593.jpg?v=1788145206"},{"product_id":"cd45-nanobeads-mouseruo-s0k0002","title":"CD45 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD45 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD45⁺ cells are magnetically labeled using CD45 Nanobeads for mice. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD45⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD45⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive fraction containing CD45⁺ cells must be further separated through a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eCD45 Nanobeads, mouse\u003c\/span\u003e\u003cspan\u003eUsed for positive selection or depletion of leukocytes from both lymphoid and non-lymphoid tissues. The CD45 antigen is expressed on all hematopoietically derived cells, except for red blood cells and platelets.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517940299,"sku":"S0K0002-1ml","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":43030517973067,"sku":"S0K0002-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c7b02ee2-5f67-4165-abe6-b235658cc5e9.jpg?v=1788094852"},{"product_id":"cd45-nanobeads-humanruo-s0k0001","title":"CD45 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD45 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD45⁺ cells are magnetically labeled using CD45 Nanobeads for human samples. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD45⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD45⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD45⁺ cells must be subjected to a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD45 Nanobeads are designed to positively select or deplete leukocytes from peripheral blood, red blood cell or platelet preparations, lymphoid tissues, tumor tissues, or non-hematopoietic tissues. The CD45 antigen is expressed on all cells of hematopoietic origin, except for red blood cells, platelets, and their precursor cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030518104139,"sku":"S0K0001-1ml","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":43030518136907,"sku":"S0K0001-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/60e937db-83f8-4a09-91f3-ceebce636a35.png?v=1788094850"},{"product_id":"human-neutrophil-isolation-kit-column-based-s0k1017","title":"Human Neutrophil Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Neutrophil Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate human neutrophils from peripheral blood. In this negative selection process, non-human neutrophils are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody‑antigen complex, while the target human neutrophils remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled human neutrophils elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of human neutrophils after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLabel-free, native neutrophils can be isolated from peripheral blood of healthy donors and used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eHuman neutrophils are the most abundant innate immune myeloid cells in peripheral blood, with a core phenotype of CD16+CD66b+. They constitute the body's first line of defense against bacterial and fungal infections and are extensively involved in key physiological and pathological processes, including inflammatory responses, immune regulation, tissue damage repair, autoimmune diseases, and the modulation of the tumor microenvironment. The isolation of high-quality, highly active, and highly pure primary neutrophils is a fundamental prerequisite for conducting cell function validation, studying inflammatory mechanisms, screening drugs, and advancing basic research in immunology.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis kit is developed based on immunomagnetic bead–based negative selection technology. It employs biotinylated antibodies to specifically label and deplete non-target cells from peripheral blood, including T cells, B cells, NK cells, monocytes, basophils, and red blood cells, without labeling or otherwise disturbing the target neutrophils throughout the process. The isolated cells can be directly used in a variety of downstream research applications, such as flow cytometry, cell activation and functional assays, inflammatory cytokine secretion analysis, and in vitro drug intervention experiments, providing a stable and reliable cell separation solution for studies of immune and inflammatory mechanisms.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eWith\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperation Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell Processing and Labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect fresh human peripheral blood, and use Red Blood Cell Lysis Buffer (without fixative, 1X) (Cat: S0D9002) to lyse red blood cells; after preparing a single-cell suspension, proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining them with AO\/PI.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer (Cat: S0D3018).\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample size; set aside a pre-sorting sample to measure the proportion of human neutrophils.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10^7\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human Neutrophil Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at 2–8℃ for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8℃ for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 minutes, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, incubate at 2–8℃ for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8℃ for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to wash the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 1 mL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer three times, collecting the target cells each time;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can handle a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column has a maximum capacity of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43045198987339,"sku":"S0K1017-100T","price":1335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0cd53983d0f1448789fe7b894662239a_97251162-aa3d-419d-8bfd-95f699e24a41.jpg?v=1788858240"},{"product_id":"starter-easyiso-separator-s0d3022","title":"Starter EasyIso Separator","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eUp to 2.5 x 108 cells\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at +10 to +35 °C.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStarter EasyIso Separator is designed for cell separation procedures using EasyIso reagents when processing up to 2.5 x 108 cells. It generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with magnetic EasyIso particles without the use of columns.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1Unit","offer_id":43092413939787,"sku":"S0D3022-1Unit","price":1250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/fb421acd-72c7-4949-bb60-4a54a2c1a27c_8f7d91fb-96d3-4593-95a0-bfaca327e5e6.png?v=1789444850"}],"url":"https:\/\/www.antbioinc.com\/collections\/magnetic-bead-cell-separation.oembed?page=5","provider":"AntBio","version":"1.0","type":"link"}