{"title":"mIHC Kits","description":null,"products":[{"product_id":"four-color-multi-label-immunofluorescence-kit","title":"Absin 4-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al. Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 99%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 88.5502%;\" colspan=\"10\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50012 (Pika) Four Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\"\u003e\u003cp\u003e\u003cstrong\u003e  Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003eTSA650\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 650 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518128857163,"sku":"abs50012-20T","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518128889931,"sku":"abs50012-100T","price":776.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518128922699,"sku":"abs50012-50T","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/de9450a6a62443b2919419a57252c9a3.png?v=1792753430"},{"product_id":"five-color-multi-label-immunofluorescence-kit","title":"Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1. Formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2. Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3. Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4. Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5. The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6. The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8. The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1. \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e (1)Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e (2)Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eIII. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1.dewaxing and hydration \u003cbr\u003e(1) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e(2) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e(3) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e(4) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(5) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2. Microwave repair antigen \u003cbr\u003e(1) The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e(2) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e(3) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e(4) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3. Quenching and sealing \u003cbr\u003e(1) Remove that residual lotion from the slide. \u003cbr\u003e(2) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(3) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4. Primary antibody incubation \u003cbr\u003e(1) Remove the blocking fluid from the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7. A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e(3) Repeat steps 4-6\u003cbr\u003e(4) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9. The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅣ. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1. Quenching, transparency, sealing \u003cbr\u003e(1) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e(2) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(3) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e(4) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(5) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2. Primary antibody incubation \u003cbr\u003e(1) Remove the closure on the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e(5) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e(6) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5. A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Repeat steps 2-4 。 \u003cbr\u003e(3) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7. The stained tissue slices were observed and analyzed under a fluorescence microscope.\u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1. Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2. In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003eMultiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003eVIII. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003efixed\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eEmbedding (frozen sample)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eAntigen repair (Paraffin Section)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSodium citrate antigen retrieval solution ( 50× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003eAntibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003eAntibody eluate ( mIHC Dedicated)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eCell permeability (Required for intracellular indicators)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eQuratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eQuenching endogenous peroxidase\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePeroxidase blocking solution ( H2O2 Method)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eclose (Serum from the same source of secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003ePrimary antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eHistochemical antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePrimary and secondary antibody diluents\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003eMulticolor kit (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eLung cancer Panel (containing primary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003ebuffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 96%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 79.3271%;\" colspan=\"11\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50013 (Pika) Five-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA700\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 700 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41938007162955,"sku":"abs50013-20T","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41938007195723,"sku":"abs50013-100T","price":1204.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107283019,"sku":"abs50013-50T","price":728.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c4bec0d23c8d490abe0ec540b77e61cc.png?v=1792753434"},{"product_id":"six-color-multi-labeled-immunofluorescence-staining-kit","title":"Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWGK Germany \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cdiv\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250820\/ef34141d61d1437aaa7ea11c1df1356c.jpg\" alt=\"\" width=\"694\" height=\"192\"\u003e\n\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 98%; height: 292px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\u003ctd style=\"width: 79.3271%; height: 54px;\" colspan=\"12\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50014 (pika) six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 76px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 76px; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 76px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 76px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 76px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e690\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 76px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 76px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 76px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 76px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 76px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 76px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 76px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e80mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5ml×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e16mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, pika universal type HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePerformance Indicator\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control). After the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative.\u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect 3 sections of the same tissue, and the staining results should be consistent. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518129840203,"sku":"abs50014-20T","price":411.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518129872971,"sku":"abs50014-100T","price":1570.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518129905739,"sku":"abs50014-50T","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d36ad5823f9f483da4497e5d7c690fa3.png?v=1792753437"},{"product_id":"sesven-color-multi-label-immunofluorescence-kit","title":"Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1. formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2. Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3. Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4. Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5. The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6. The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8. The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1. \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4. \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eIII. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1. dewaxing and hydration \u003cbr\u003e（ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e（ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e（ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e（ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e（ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2. Microwave repair antigen \u003cbr\u003e（ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e（ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e（ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e（ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3. Quenching and sealing \u003cbr\u003e（ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e（ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e（ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4. Primary antibody incubation \u003cbr\u003e（ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e（ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5. Secondary antibody incubation \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e（ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6. Fluorescence staining to amplify the signal \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e（ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7. A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e（ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e（ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e（ 3 ) Repeat steps 4-6\u003cbr\u003e（ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9. The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1. Quenching, transparency, sealing \u003cbr\u003e（ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e（ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e（ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e（ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e（ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2. Primary antibody incubation \u003cbr\u003e（ 1 ) Remove the closure on the slide. \u003cbr\u003e（ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3. Secondary antibody incubation \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e（ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4. Fluorescence staining to amplify the signal \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e（ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e（ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e（ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5. A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e（ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e（ 2 ) Repeat steps 2-4 。 \u003cbr\u003e（ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7. The stained tissue slices were observed and analyzed under a fluorescence microscope.\u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1. Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2. In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003eMultiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003eVIII. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003efixed\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eEmbedding (frozen sample)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eAntigen repair (Paraffin Section)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSodium citrate antigen retrieval solution ( 50× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003eAntibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003eAntibody eluate ( mIHC Dedicated)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eCell permeability (Required for intracellular indicators)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eQuratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eQuenching endogenous peroxidase\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePeroxidase blocking solution ( H2O2 Method)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eclose (Serum from the same source of secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003ePrimary antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eHistochemical antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePrimary and secondary antibody diluent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003eMulticolor kit (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eLung cancer Panel (containing primary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003ebuffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 78.9156%;\" colspan=\"13\" width=\"1064\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50015 (Pika) Seven-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e480\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e690\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e100mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e10μLl\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 480 、 TSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, pika universal type HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2~8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107315787,"sku":"abs50015-20T","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521814962251,"sku":"abs50015-100T","price":2046.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41521814995019,"sku":"abs50015-50T","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d4b127f4e0554a5daba97bbb65ce9238.png?v=1792753442"},{"product_id":"stripping-buffer","title":"Antibody Eluent (for mIHC)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eReady-to-use, no dilution required. Antibody elution: Preheat the antibody eluate at 37 °C, add a small amount of antibody eluate dropwise to cover the sample, and place it at room temperature for 3 to 5 minutes; Discard the eluate, add a sufficient amount of eluate dropwise again to cover the sample (the amount of eluate should be slightly bulged on the film, generally 150 ~ 300μL), 37 °C for 10 ~ 40min (generally 20min is enough); PBS wash 3 times (fixative can be added to briefly fix again).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. The effect of antibody elution depends on the thickness of the section, elution temperature and time, and the type of primary antibody (elution difficulty: structural membrane protein and cytoskeletal protein \u0026gt; cytoplasmic protein \u0026gt; nuclear protein). 4-8 μm slices can usually be treated in a wet box at 37 °C for 10-40 minutes.\u003cbr\u003e2. Some antibodies, such as E-cadherin and Tuj1, bind to the antigen very strongly, and the elution temperature can be increased to 50 °C, or the elution time can be extended to 60min.\u003cbr\u003e3. If there is a problem with the elution of some antibodies, you can reduce the dilution ratio of the primary antibody, or put these antibodies in the last round of TSA labeling.\u003cbr\u003e4. Appropriate protective measures should be taken to avoid contact between the reagent and the skin and eyes. If you accidentally come into contact with sensitive areas, rinse immediately with plenty of water.\u003cbr\u003e5. The activity of reagents beyond the validity period may be reduced, so please use them within the validity period of the reagent.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2 ~ 8 ℃, the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe antibody eluate is used for paraffin sections, frozen sections, bone tissue sections, cell crawling sections and other samples, and the antibody elution during TSA staining.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"30mL","offer_id":41518207828043,"sku":"abs994-30mL","price":150.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-mihc-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}