{"title":"IHC Detection Kits","description":null,"products":[{"product_id":"anti-infection","title":"Ready to use high-efficiency immunohistochemical secondary antibody kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Following the conventional Western blot operation, after the second antibody is incubated, during the last washing, according to the size of the membrane, mix 0.5ml of solution A and 0.5ml of solution B every 10 cm2 of the membrane, and prepare a luminescent detection working solution. 2. Use flat headed tweezers to remove the membrane, with the lower edge of the membrane gently touching the absorbent paper to remove excess liquid from the membrane. The protein side of the film should be facing upwards and placed on a clean cling film (some commercially available cling films may quench fluorescence when wrapping imprinted films, so high-quality cling films should be chosen). Transfer the prepared luminescent detection solution onto the protein film using a pipette, cover it evenly, and incubate at room temperature for 2 minutes. 3. Use flat headed tweezers to grip the protein film, gently touching the lower edge of the film with absorbent paper to remove excess liquid from the film. The protein side of the film is facing upwards and wrapped in a clean cling film. Gently push out the bubbles between them and fix them in the X-ray cassette. 4. Take an X-ray film in the darkroom and place it on the wrapped film. Close the cassette and expose it for 30 seconds to 1 minute. Immediately develop and fix, shorten or extend the exposure time of the next X-ray film based on its exposure intensity (for weak signals, the exposure time can be extended to several hours).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtocol\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. According to the routine Western blot operation, after the incubation of the secondary antibody and the last wash, according to the size of the membrane, 0.5ml solution A and 0.5ml solution B were mixed per 10 cm2 membrane to prepare the luminescence detection working solution. \u003cbr\u003e\n2 Remove the membrane with flat-tipped tweezer and gently touch the lower edge of the membrane with absorbent paper to remove excess liquid from the membrane. The protein side of the membrane is facing up and placed on clean plastic wrap (some commercial plastic wrap may quench fluorescence when wrapping the imprinted film, and high-quality plastic wrap should be selected). The prepared luminescent detection solution was transferred to the protein membrane with a pipette so that it was uniformly covered and incubated for 2 min at room temperature. \u003cbr\u003e\n3. Hold the protein membrane with a flat-headed tweezer, and the lower edge of the membrane gently touches the absorbent paper to remove excess liquid on the membrane. Membrane protein on the face, wrapped in a clean plastic wrap. Gently expel the air bubbles in between and fix them in the X-ray cassette.\n\u003cbr\u003e\n4. Take an X-ray film in the dark room and place it on the wrapped film, close the cassette, and expose it for 30 seconds to 1 minute. \"The image is developed immediately, and the exposure time of the next radiograph is shortened or extended depending on its exposure intensity (for weak signals, the exposure time can be extended to several hours).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003epackaging: \u003c\/strong\u003e5ml (can be used for 50 pieces)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eapplicable: \u003c\/strong\u003eprimary antibody is from mouse or rabbit\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003echromogenic substrate: \u003c\/strong\u003edab (benzidine)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ereagent composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" width=\"250\" cellspacing=\"1\" cellpadding=\"1\" align=\"left\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\" width=\"100\" height=\"20\"\u003e\u003cstrong\u003ecomponent number \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"200\"\u003e\u003cstrong\u003ecomponent name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"50\"\u003e\u003cstrong\u003especification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"50\"\u003e\u003cstrong\u003equantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\" width=\"100\"\u003eA\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eHydrogen peroxide\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eB\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eSuper sealing fluid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eC\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003ePrimary antibody amplification agent\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eD\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eEnzyme labeled secondary antibody polymer\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eE\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eDab a liquid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eF\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eDAB B fluid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e160ul\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u0026amp;nbsp\u003cstrong\u003ereagents not provided but required: \u003c\/strong\u003eprimary antibody (from mouse or rabbit), PBS (phosphate buffer)\u003c\/p\u003e\n\u003cp\u003ethis kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003estorage and validity period \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8° C save. Each component can be stored for at least 18 months\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eoperation steps: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003enote: all steps are operated at room temperature. Adding reagent can be titrated directly in a drop bottle or with a pipette. One drop =30-40ul\u003c\/p\u003e\n\u003cp\u003e1  Dewaxed and hydrated tissue sections\u003c\/p\u003e\n\u003cp\u003e2  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e3  According to the special requirements of the applied primary antibody, the tissue sections were pretreated\u003c\/p\u003e\n\u003cp\u003e4  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e5  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eA solution \u003c\/span\u003e\u003c\/strong\u003ewas incubated for ten minutes to block endogenous peroxidase to reduce nonspecific background staining\u003c\/p\u003e\n\u003cp\u003e6  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e7  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eB solution \u003c\/span\u003e\u003c\/strong\u003ewas incubated for five minutes to reduce nonspecific staining. Note: this step should not exceed ten minutes; If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted\u003c\/p\u003e\n\u003cp\u003e8  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e9  Add primary antibody and incubate at room temperature or 37 ℃ for 20 min\u003c\/p\u003e\n\u003cp\u003e10  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e11  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eC solution \u003c\/span\u003e\u003c\/strong\u003eincubation for ten minutes\u003c\/p\u003e\n\u003cp\u003e12  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e13  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eIncubate with D solution \u003c\/span\u003e\u003c\/strong\u003efor ten minutesNote: this liquid is sensitive to light, and pay attention to avoiding light\u003c\/p\u003e\n\u003cp\u003e14  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e15  Prepare fresh substrate solution: add 30ul of \u003cstrong\u003e\u003cspan style=\"color: #e03e2d;\"\u003eF\u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #ff0000;\"\u003e\u003cstrong\u003e liquid \u003c\/strong\u003e\u003c\/span\u003eand 1ml of \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eE solution \u003c\/span\u003e\u003c\/strong\u003emix well to prepare a fresh substrate solution (the substrate solution can be stored for 2 weeks)\u003c\/p\u003e\n\u003cp\u003e16  Add 100ul of fresh substrate solution and incubate for five minutes\u003c\/p\u003e\n\u003cp\u003e17  Deionized water flushing\u003c\/p\u003e\n\u003cp\u003e18  Counterstain, seal\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLiquid A; liquid B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":41518202519627,"sku":"abs957-5mL","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ea3ea837c23f4f29a11218e1abb7f547.png?v=1792756840"}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-ihc-detection-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}