{"title":"ELISA Kits","description":null,"products":[{"product_id":"human-il-13-elisa-kit","title":"Human IL-13 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 6000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (6000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240123\/5ecb483ff0cd4b7ca728e700208ac65d.png\" alt=\"\" width=\"431\" height=\"251\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240123\/b5326ad2fe63456a8b20d1c60a815389.jpg\" alt=\"\" width=\"304\" height=\"237\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of human IL-13 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated by high-purity recombinant human IL-13 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with high concentrations of human IL-13, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant human IL-13 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IL-13. Interference with human IL-13 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IL-13 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 70px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 29px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 29px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eIL-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 12px;\"\u003eIL-4R\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 12px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u0026lt; td style = \"width: 45.9864%; height:22px; \"\u0026gt; Add the correct reagent exactly according to the instruction steps\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not replaced during sample addition, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eHemolysis, storage for too long, incomplete agglutination, contamination by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2.324pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-13 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-13 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eALRHMGC116789, BHR1interleukin-13, IL13, IL-13, IL-13 MGC116788, interleukin 13, MGC116786, NC30, P600, human interleukin 13 ELISA kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-13 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-13 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-13 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-13 (interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 inhibits the production of pro-inflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class conversion to IgE, and upregulates CD23\/Fc epsilon RII. IL-13 signaling is transmitted through a receptor complex containing IL-13Rα1 and IL-4Rα1. This complex also functions as a type 2 IL-4 receptor. IL-13 Rα2 binds to IL-13 and prevents IL-13 from signaling through the IL-13 Rα1\/IL-4 Rα complex. IL-13-bound IL-13R2 can directly promote the development of tumor cell invasiveness and tissue fibrosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e93.75pg\/mL-6000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293304856651,"sku":"abs510016-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f62152aefbc14ef29936eca0d76e6b88.jpg?v=1792753314"},{"product_id":"human-il-15-elisa-kit","title":"Human IL-15 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 1000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (1000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/053566c600264a86867ff3858e3dfb0c.jpg\" alt=\"\" width=\"311\" height=\"245\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of human IL-15 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated by high-purity recombinant human IL-15 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with high concentrations of human IL-15, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant human IL-15 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IL-15. Interference with human IL-15 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IL-15 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 47.5817%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-15R\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u0026lt; tr style = \"height: 22 px; \"\u0026gt;2Low application temperature and short timeIf the temperature is low, extend the incubation time and color development time\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-15 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-15 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 15 detection kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 384px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-15 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-15 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-15 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 32px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 32px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 32px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-15 (Interleukin15) is a cytokine that induces or enhances the differentiation, maintenance or activation of a variety of T cell subsets, including NK, NKT, Th17, Treg, and CD8 + memory cells. It can also induce dendritic cell differentiation and inflammatory activation, exhibit anti-tumor activity, and inhibit lipid deposition in adipocytes. IL-15 interacts with complexes of IL-15Ralpha and IL-2Rbeta on the cell surface and with common γ chains on adjacent cells. This transposition mechanism causes cells to respond to IL-15 even if they do not express IL-15R. Ligation of membrane-associated IL-15\/IL-15Ralpha complexes also induces reverse signaling, which promotes activation of IL-15\/IL-15Ralpha-expressing cells. The activity of free IL-15 is limited by its relationship to soluble IL-15R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293304922187,"sku":"abs510018-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0011d97b22f2489284e86b1b129d8420.jpg?v=1792753317"},{"product_id":"human-il-22-elisa-kit","title":"Human IL-22 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.15pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSam\u003cspan style=\"color: #000000;\"\u003eple \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e\u003c\/span\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e  1.8 pg \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   31.3 - 2000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e 82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/927fa19e7bf84c67a1176eee1603f24c.jpg\" alt=\"\" width=\"600\" height=\"466\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: IL-22\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIl-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305020491,"sku":"abs510020-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d0382f3ac6b044e694c0420f34232043.jpg?v=1792753325"},{"product_id":"human-il-27-elisa-kit","title":"Human IL-27 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.26pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human IL-27 monoclonal antibody is coated on the microplate. IL-27 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-27 multi antibody was added to combine with IL-27 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human IL-27 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e156 - 10000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/fc4d82c317684056bf12df44a5288b3f.jpg\" alt=\"\" width=\"300\" height=\"236\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIL-27 is composed of ebi3 (associated with the P40 subunit of IL-12 and IL-23) and p28 (associated with the p35 chain of IL-12)Composed of heterodimeric cytokines. IL-27 is expressed by monocytes, endothelial cells, and dendritic cells through a receptor complex consisting of IL-27 R alpha\/wsx-1\/tccr and gp130. IL-27 induces IL-12 receptor expression on naive cd4+ T cells, sensitizing them to subsequent IL-12-induced Th1 development. It also acts as an anti-inflammatory cytokine by limiting the activity of T and NKT cells.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305118795,"sku":"abs510021-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/435ba4b405a54c968b342e5b6898e40d.jpg?v=1792753327"},{"product_id":"human-fgf-acidic-elisa-kit","title":"Human FGF Acidic ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human FGF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.19 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e125 - 8000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e96-113%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/bdf12b13825c4695804398cb0c23b9f7.jpg\" alt=\"\" width=\"300\" height=\"232\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFibroblast growth factor acidic (FGF acidic), also known as FGF-1, endothelial cell growth factor (ECGF) and heparin binding growth factor-1 (HBGF-1) are non glycosylated 17-18 kDa polypeptides secreted by a variety of cell types. This molecule is synthesized as an amino acid without obvious signal peptide sequence(AA) protein. Therefore, this ruled out the possibility that it was secreted through the canonical er\/ Golgi pathway. However, it seems to have a unique secretion pathway including heat shock protein, phosphatidylserine, synaptotagmin-1. The result of acidic secretion of FGF is that extracellular disulfide bonds connect homodimers, and its covalent bonds may be destroyed by reducing agents to release bioactive FGF acidity. Unlike FGF-2, acidic FGFs are known to have a 5'alternate start site. The acidic FGF of a 60 AA splice variant has been reported to be a full-length molecule consisting of the first 57 amino acids plus three additional C-terminal amino acids. In experiments, it can bind FGF receptors, but will not activate them. Acidic FGF has a nuclear localization sequence consisting of aa residues 21-27. Acidic FGF exhibits 95% AA properties for mice and rats and 92% AA properties for cattle. Cells known to express acidic FGFs include mammary epithelial cells and neurons (motor and sensory), skeletal muscle and smooth muscle cells, renal proximal tubular cells, endothelial cells, macrophages, keratinocytes, fibroblasts. The FGF family has five receptors (FGF R1-R5). The first four receptors are transmembrane tyrosine kinase receptors, and FGF R5 is a member of IgSF without tyrosine kinase domain. Notably, acidic FGFs have been reported to bind receptors 1 to 4, but not FGF R5, with FGFs. Acidic FGFs can activate tyrosine kinase binding or use the signal pathway of ligand receptor internalization to reach organelles and nuclei in cells. Receptor activation is associated with concurrent FGF heparin interactions. Heparin can bind with acidic FGF to inhibit or activate the receptor. When activated, heparin appears to be a central component connecting the two fgf\/fgf r complexes. This is an approximation of two FGF r molecules to initiate receptor dimerization and signal transduction. Acidic FGFs also bind heparin on the cell surface, with internalization but no cell activation. Acidic FGF is famous for its mitotic activity on endothelial cells. Activation and internalization of membrane receptors are necessary for complete mitosis. Other cells that proliferate in response to acidic FGF include fibrocytes, muscle cells, hepatocytes, mammary epithelium and fibroblasts.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305348171,"sku":"abs510022-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ec205912bf894053b075e538838c66b2.jpg?v=1792753331"},{"product_id":"human-fgf-basic-elisa-kit","title":"Human FGF basic ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ebasic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human FGF basic antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the FGF basic present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cstrong\u003eSample \u003c\/strong\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e\u003c\/span\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e   0.089-0.740 pg \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e   94-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/cf9fd7d18f4943b5b6fe4466e6a5b998.jpg\" alt=\"\" width=\"600\" height=\"466\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: FGF acidic\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFGF basic, also known as FGF-2(fibroblast growth factor-2) or HBGF-2(heparin-binding growth factor-2), is the most intensively studied of the 22 mitogenic proteins in the FGF family.FGF family members share 35-60% amino acids (aa), but only acidic and alkaline FGF lack signal peptides,which are secreted by another pathway. The 18 kDa FGF basic subtype can be found in the cytoplasm and nucleus and is also secretory.It may be present in storage tanks of heparin sulphate proteoglycans (HSPG) inside or on the cell surface.Transcription from alternate initiation sites produces a 21-23 kDA form, found only in the nucleus.\u003c\/p\u003e\n\u003cp\u003eHuman FGF basic sequences at 18 kDa had aa homology of 97% and 99%, respectively, with mouse\/rat and cattle\/sheep FGF basic sequences. However, the destruction of basic FGF genes in mice resulted in relatively mild cardiovascular, skeletal, and neurophenotypes, suggesting that other members of the FGF family compensated for this.Transgenic basal overexpression mainly affects bone development and mineralization.\u003c\/p\u003e\n\u003cp\u003eFour FGF tyrosine kinase receptors (FGF R) and their splicing variants showed differential binding of FGF.FGF basic preferentially binds FGF R1c and 2C with picoluminal affinity.FGF basic also has a number of other binding partners that can fine-tune FGF basic activity depending on their location and number. These include heparin, integrin V 3, soluble FGF R1, FGF binding protein, free gangliosides, platelet-reactive protein, pentammeric protein 3, fibrinogen, 2-macroglobulin, platelet-derived growth factor and platelet factor-4, all of which bind in nanomolar affinity. These molecules can act as co-receptors or adhesion chaperones of the cells, bait or reservoir in the extracellular matrix, while scavengers or chaperones can act as free proteins. FGF basic binding to cell surface HSPG is particularly critical and is necessary for binding, dimerization, and activation of FGF R.\u003c\/p\u003e\n\u003cp\u003eFGF basic regulates normal processes such as angiogenesis, wound healing, tissue repair, learning and memory, and embryonic development and differentiation of the heart, bone, and brain.It is upregitated by inflammatory mediators such as TNF-α, IL-1β, IL-2, PDGF and nitric oxide.Many human tumors express basic FGF, which may be related to tumor angiogenesis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305413707,"sku":"abs510023-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5de707cd016445b29ccbbb3a0eddb86b.jpg?v=1792753334"},{"product_id":"human-g-csf-elisa-kit","title":"Human G-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.46pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human G-CSF monoclonal antibody is coated on the microplate. The G-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human G-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plate, standard, anti human G-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.3 - 2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e96-103\u003cspan style=\"font size: 13.3333px;\"\u003e%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/c02ab025b24a485688e2b368c9fd0d77.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation, and activation of hematopoietic cells in the neutrophil lineage. Mature human G-CSF is a 178 amino acid (AA)Of O-glycosylated proteins, containing two intrachain disulfide bonds. In humans, alternative splicing generates a second isoform with a 3-amino acid deletion. Mouse and human G-CSF share 76% amino acid sequence identity, and the two proteins show species cross reactivity. G-CSF is produced by activated monocytes and macrophages, fibroblasts, endothelial cells, astrocytes, neurons, and bone marrow stromal cells. In addition, various tumor cells constitutively express G-CSF.\u003c\/p\u003e\n\u003cp\u003eHuman G-CSF receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to the haematopoietin receptor superfamily. The mature protein consists of a 603 amino acid extracellular domain (ECD), a 23 amino acid transmembrane fragment, and a 186 amino acid cytoplasmic domain. ECD contains an N-terminal Ig like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Alternative splicing of human G-CSF r generates other isoforms, including potentially soluble forms of the receptor. The ECDs of mouse and human G-CSF r share 63% amino acid sequence identity. G-CSF r forms a complex with the ligand in a 2:2 ratio. It is expressed on monocytes, neutrophils, megakaryocytes, platelets, bone marrow progenitor cells, trophoblasts and placentas, endothelial cells, and various tumor cell types.\u003c\/p\u003e\n\u003cp\u003eG-CSF is an important regulator of granulopoiesis in vivo, and mutations in G-CSF R are associated with congenital neutropenia. G-CSF can support the growth of multilineage hematopoietic progenitor cells and mobilize them from the bone marrow into the blood. G-CSF enhances the functional capacity of mature neutrophils and supports their survival by limiting the apoptosis rate. G-CSF also enhanced m-csf-induced monocyte generation caused by hematopoietic progenitor cells and stimulated the proliferation of peripheral Th2 inducing dendritic cells. It promotes the development of T cell immune tolerance and tissue recovery after myocardial infarction and cerebral ischemia. The human G-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure human G-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human G-CSF expressed in E. coli and antibodies against the recombinant protein. Recombinant human G-CSF has been shown to be accurately quantified. The results obtained using natural human G-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of natural human G-CSF.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305446475,"sku":"abs510024-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/53129f1fa0a2431eb93b0dba1a41200b.jpg?v=1792753336"},{"product_id":"human-gm-csf-elisa-kit","title":"Human GM-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.44pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti human GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human GM-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740  Pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6 - 1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85-104%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/8aec979acf4a4811bc45e0baf90da1e7.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte macrophage colony stimulating factor (GM-CSF), also known as csf-2, is a common β chain (β c)The polytrophic 30 kDa member of the cytokine family also includes IL-3 and IL-5. GM-CSF adopts \u0026amp;alpha with two intrachain disulfide bonds-Helical configuration. It is secreted by a variety of activated immune, mesenchymal, and epithelial cell types and circulates in the form of variable glycosylated monomers. During inflammation, it is upregulated in a variety of cell types, including encephalitic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF has 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The high affinity receptor of GM-CSF is bound by a 50 kDa ligand α Subunit (gm-csfrα)And 120 kDa signal transduction β C composition. According to reports, the stoichiometry of functional GM-CSF receptor is GM-CSF, gm-csfrα And β The 2:2:2 complex of C. It is worth noting that β The C subunit is shared by the receptor complex of IL-3 and IL-5, and IL-5 may pass through gm-csfrα And β C signaling. GM-CSF can also utilize syndecan-2 as a co receptor (14). GM-CSF has many functions. It induces the production of monocytes, neutrophils, and eosinophils from CD34 + stem cell precursors. It can synergize with IL-4 or Flt-3 ligands to induce the development and maintenance of bone marrow and dermal dendritic cells. It also functions as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. Human GM-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure the levels of human GM-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human GM-CSF expressed in E. coli and antibodies against recombinant factors. Recombinant human GM-CSF has been shown to be accurately quantified. The results obtained using natural human GM-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of human GM-CSF.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305512011,"sku":"abs510025-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1ea541b3b7644137bfae58497aac572e.jpg?v=1792753339"},{"product_id":"human-il-4-elisa-kit","title":"Human IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241209\/fecf5a9262c248099d03b1e0b94e202e.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241209\/c5ff990e35494e709c6775531d4da833.jpg\" alt=\"\" width=\"428\" height=\"338\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of Human IL-4 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated with high purity recombinant Human IL-4 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with a high concentration of Human IL-4, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant Human IL-4 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-4. Interference with Human IL-4 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant Human IL-4 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u0026lt; td style = \"width: 33.32%; height: 10 px; \"width =\" 33.3200% \"\u0026gt; IL-4Rα\u003ctable style=\"border-collapse: collapse; width: 65.6055%; height: 185px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 31px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eOther recombinant proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003eCommon γ Chain\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003eCommon γ Chain\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003ebovine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003ecanine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003egp130\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003ecotton rat IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-2 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eequine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003efeline IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eporcine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003eIL-4 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003erabbit IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003erat IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003erhesus macaque IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-6R\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα1\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα2\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα1\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα2\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003cbr\u003e\u0026lt; tdstyle = \"width: 41.4459%; height: 22px;\" \u0026gt; Substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) contaminated or exposed to metal ions or oxidants\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-4 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-4 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. The reaction was stopped by adding a stop solution, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4, IL-4B_cell stimulatory factor 1, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, pitrakinra\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 362px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-4 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-4 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-4 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-4 is a secreted protein of 129 amino acids. IL-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils. By acting on multiple types of cells, IL-4 has multiple immune response regulatory functions. IL-4 is involved in several B cell activation processes. It is also a co-stimulator of DNA synthesis. Induction of MHC class II molecule expression on dormant B cells. IL-4 can enhance the secretion and cell surface expression of IgE and IgG1. It also regulates the expression of low-affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes. IL-4 positively regulates IL31RA expression in macrophages.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3 pg\/mL- 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305643083,"sku":"abs510017-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4df3fb8d3d94727abfe362cf915ff42.jpg?v=1792753359"},{"product_id":"human-porcine-insulin-elisa-kit","title":"Human \/ Porcine Insulin ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti- Human \/ Porcine Insulin antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the Human \/ Porcine Insulin present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eS\u003cspan style=\"color: #000000;\"\u003eample \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e   2.15 pmol \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1000 pmol\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e   85.0-108.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/c36b0514d4ff44bc8a1eaf4cf421cf28.png\" alt=\"\" width=\"345\" height=\"273\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: Insulin\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInsulin and IGF are members of the insulin molecule family. IGF-I and II are structurally homologous to proinsulin and can be thought of as an exaggerated inverted G shape. The G part of the chain is inchain disulfide bonded. Single-stranded proinsulin peptide is composed of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589) and 21 amino acid residues of A chain (aa 90110).The removal of C peptide by proteolysis results in the formation of mature insulin, which is the heterodimers formed by disulfide bonds between A and B chains. Circulating C peptide levels are elevated in hyperinsulinemia, obesity and type 2 diabetes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical hints: \u003c\/strong\u003eFor research use only, not for in vitro diagnosis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305774155,"sku":"abs510030-96T","price":450.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-il-2-elisa-kit","title":"Human IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1.  Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2.  High precision liquid dispenser and disposable tip \u003cbr\u003e3.  Distilled or deionized water \u003cbr\u003e4.  Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2.  Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand  \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 1000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 1000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240117\/dbd403ac84e44c85925cd9baa5910f7e.png\" alt=\"\" width=\"489\" height=\"277\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Prepare all required reagents and standards; \u003cbr\u003e2.  Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3.  Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4.  Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5.  Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6.  Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7.  Repeat th 5 Step washing operation; \u003cbr\u003e8.  Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9.  Repeat th 5 Step washing operation; \u003cbr\u003e10.  Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11.  Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12.  After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13.  Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240117\/20605d9cc3dd4d47a44c576150ba3234.jpg\" alt=\"\" width=\"357\" height=\"278\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Recovery: Different levels of human were spiked in cell culture medium samples IL-2 The recovery rate was determined. The recoveries range from 102-104% , the average recovery was in 103% 。 \u003cbr\u003e2.  Sensitivity: Human IL-2 The lowest measurable dose ( MDD ) is generally less than 3.24pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e3.  Correction: This ELISA High purity recombinant human expressed by Escherichia coli IL-2 Corrected by protein. \u003cbr\u003e4.  Linearity: 4 Different samples were spiked with high concentrations of human IL-2 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 61.9296%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003e Dilution factor \u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003e Recovery ( % ） \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 10px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 10px;\"\u003e83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e5.  Specificity: This ELISA Method can detect natural and recombinant human IL-2 Egg whites. The following factors were mixed with diluent ( 1× ) formulated into  50ng\/mL Concentration to detect human IL-2 Cross-reactivity of. Will 50ng\/mL Interference factors incorporated into the intermediate range of recombinant human IL-2 In the reference substance, to detect the effects of human IL-2 Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 132px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e Recombinant human protein \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e Recombinant mouse protein \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2sRα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rβ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Improper storage of kits; Mixing reagents of different kits \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Purchase new kits and pay attention to storage conditions; Not mixable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Low application temperature and short time \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e If the temperature is low, extend the incubation time and color development time \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Wrong addition or missed addition of reagents \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Add the correct reagent strictly according to the instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e The container used to prepare the solution is not clean or there is a problem with the water \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Use clean containers and qualified distilled water \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e In the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Follow the instructions strictly \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Heterogeneity of reagent temperature \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e All reagents should be equilibrated at room temperature 30 minute \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003e Detecting antibodies and \/ Or HRP Concentration too low \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003e Refer to the instructions, do not dilute at will \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2.  Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Less washing times, insufficient \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Wash as per instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Substrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Use clean containers and qualified distilled water during preparation; Store protected from light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Incubation temperature is high and \/ Or too long \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Controlling the temperature and time of incubation and final enzymatic reaction \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e The gun tip was not changed during sample loading, resulting in cross-contamination \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Change the gun head for each sample \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Cross contamination of nearby holes \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Vertical clapper, use suitable clapper paper to avoid paper scraps in the hole \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Presence of endogenous interfering substances in sample \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Estimate possible infectious substances and perform corresponding treatment \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003e Samples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003e Avoid hemolysis, contamination, long storage and other phenomena \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 5. Experimental flow chart \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.713pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-2 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ealdesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF, Human interleukin 2 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-2 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-2 Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is an α-helix polypeptide with a molecular weight of 15-18kDa with different glycosylations, which belongs to the common γ-chain (γc) cytokine family members. It exists as a monomer with a very short half-life (\u0026lt; 30min). The precursor of human IL-2 has 153 amino acids, including a 20 amino acid signal sequence, and a 133 amino acid mature polypeptide. IL-2 mature protein contains an O-type glycosylation site at threonine position 3 and three cysteines, of which the intra-chain disulfide bond formed by two cysteines is essential for IL-2 activity. The mature human IL-2 amino acid sequence shares 73%, 66%, 78%, and 97% homology to IL-2 in dog, rat, cat, and macaque, respectively. Although human IL-2 has only 60% amino acid homology with highly polymorphic mouse IL-2, human IL-2 is also biologically active on mouse cells. Cells reported to secrete IL-2 include γδ T cells, activated conventional CD4 + and CD8 + T cells, neurons, microglia, hematopoietic stem cells.\u003cbr\u003eThe IL-2 receptor (IL-2R) consists of three subunits, a 55 kDa CD25\/IL-2Rα chain, a 70 kDa CD122\/IL-2Rβ chain, and a 65 kDa CD132\/γc chain. IL-2 first binds to CD25, and the two-subunit complex formed recruits CD122 and CD132, forming a signal complex with four subunits. In addition to forming a complex with IL-2, CD122\/IL-2Rβ can also form a four-subunit signaling complex with IL-15. CD132\/\u003cs\u003e \u003cen\u003e Also acts as a receptor for IL-4, IL-7, IL-9, IL-15 and IL-21 signaling.\u003cbr\u003eIn vitro studies have shown that IL-2 plays an important role in T cell activation and expansion. In vivo, IL-2 is key to the development, maintenance, and function of regulatory T cells (Tregs), and regulatory T cells can provide protection against autoimmune diseases. In addition, IL-2 can also promote autoimmune inflammation in its target organs by regulating the expression of T cell trafficking genes and the production of Th2 cytokines. In CD8 + T cell subsets, IL-2 is a necessary key to obtaining an optimal first-order response and differentiation into terminal effector cells. IL-2 also promotes the development and activation of CD8 + T cells into memory cells.\u003c\/en\u003e\u003c\/s\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305905227,"sku":"abs510001-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/28e08912821d4ab9b9b3c6a9b4306b07.jpg?v=1792753416"},{"product_id":"human-il-6-elisa-kit","title":"Human IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter using 10 wells, 10 uL of the detection antibody at 100 times the working concentration was taken and volume to 1 mL using diluted buffer (1 ×) to obtain 1 mL of the detection antibody at 1 × working concentration.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 600pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (600 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/072d4b0366654b2d907a0b473832bae3.png\" alt=\"\" width=\"432\" height=\"248\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 570nm or 630nm as the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/86a1a6e09102494cb831b1787e3b6b89.jpg\" alt=\"\" width=\"320\" height=\"252\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human IL-6 were spiked into cell culture medium samples and the recovery rate was determined. The recoveries ranged from 93 to 101%, with an average recovery of 98%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human IL-6 is generally less than 1.56 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Linearity: 4 different samples were spiked with a high concentration of Human IL-6, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eRecovery (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant Human IL-6 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-6. Interference with Human IL-6 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant Human IL-6 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.4573%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px; width: 24.0666%;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003esgp130\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6 sR\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-2 Rγ\u003c\/td\u003e\u0026lt; td style = \"width: 24.0666%; height: 22px;\" \u0026gt;\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eIL-6 sR\/sgp130\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e5. Experimental flow chart\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.56pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-6 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell stimulatory factor-2, B-cell differentiation factor, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFN-beta-2, IL6, IL-6, IL-6B-cell stimulatory factor 2, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A, Human interleukin 6 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-6 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-6 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-6 (IL-6) is a multifunctional cytokine with α-helix structure, 22-28kDa phosphorylation and varying degrees of glycosylation. It plays an important role in the acute phase of disease response, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. Mature human IL-6 has 183 amino acids and 41% homology to mouse and rat IL-6. Alternative splicing within IL-6 produces a variety of isomers, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8 + T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endotheliin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult glial cells, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B cells, and islet beta cells. IL-6 production is usually controlled by glucocorticoids, catecholamines, and secondary sex steroids, and is generally associated with cellular activation. IL-6 in normal human blood is in the range of 1 pg\/mL, slightly increased during menstrual period, severely increased in the middle and late stages of some cancers, and significantly increased after major surgery.\u003cbr\u003eIL-6 elicits cellular signaling through a cell surface receptor, which is a heterodimeric complex consisting of a ligand-binding subunit (IL-6 receptor) and a signal-transferring subunit gp130. Binding of IL-6 to the IL-6 receptor triggers binding of the IL-6 receptor to gp130 and dimerization of gp130. gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptors are produced by alternative splicing and protease cleavage. Through trans-signaling mechanisms, soluble IL-6 and IL-6 receptor complexes can elicit responses in cells that lack IL-6 receptors on their surface but express gp130. The expression of IL-6 receptor is mainly restricted to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Since the gp130 molecule is very widely expressed, trans-signaling enables a wider range of cell types to respond to IL-6. The soluble gp130 spliceosome prevents trans-signaling of IL-6\/IL-6R, but not signaling by other cytokines using the gp130 molecule as a co-receptor.\u003cbr\u003eTogether with tumor necrosis factor α (TNFα) and IL-1, the acute inflammatory response caused by IL-6 plays an almost unique role in fever and acute inflammatory response of the liver. It also plays an important role in the transformation of acute inflammation to acquired immunity or chronic inflammatory diseases. IL-6 dysregulation can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving trans-signaling of IL-6. In the presence of transforming growth factor TGF-β, IL-6 plays an important role in the differentiation of naïve T cells into Th17 inflammatory cells. IL-6 regulates bone resorption and is a major contributor to inflammatory joint damage in rheumatoid arthritis by promoting the activity of Th17 inflammatory cells. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects, such as skeletal muscle secretion of IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes and induces B cells to mature into plasma cells and immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation-related carcinogenesis, such as colitis-related cancers.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent (1 ×) in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e9.38pg\/mL-600pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305937995,"sku":"abs510003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5bdc318eaa694e30afae84b8c6a70e2f.jpg?v=1792753418"},{"product_id":"human-vegf-elisa-kit","title":"Human VEGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, at a speed of 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240816\/e408a92bc74747eba4853a26c8b4c79f.jpg\" alt=\"\" width=\"373\" height=\"295\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human VEGF were spiked into cell culture medium samples and the recovery rate was determined. The recoveries ranged from 113 to 135%, with an average recovery of 121%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human VEGF is generally 7.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human VEGF protein expressed by sf-21 insect cells.\u003cbr\u003e\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of Human VEGF, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 极速赛车开奖记录\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Sensitivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;7.8pg\/mL\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Species Reactivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Human\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human VEGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference corrected wavelength of 540 nm or 570 nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Synonym\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGF, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Please use within the expiration date of the kit\u0026lt;\/strong\u0026gt;\u0026lt;br \/\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman VEGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman VEGF Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman VEGF detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor, is an effective regulator of angiogenesis and angiogenesis in fetus and adults. Vascular endothelial growth factor belongs to the PDGF family, a family of proteins characterized by the presence of eight conserved cystine residues at the structure of the cystine knot and the dimer bound by antiparallel disulfide bonds. After alternative cleavage and sequence length of amino acids, humans express different isoforms, including: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189 and VEGF206, among others. Among them, VEGF165 is the highest expression isoform, followed by VEGF121 and VEGF189. With the exception of VEGF121, the other isoforms contain a basic heparin-binding region and do not diffuse freely. Human VEGF165 has 88% homology to the corresponding protein amino acid sequences of mouse and rat. VEGF is expressed in various cells and tissues, including skeletal muscle cells and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34 + stem cells, endothelial cells, fibroblasts, vascular smooth muscle cells, and the like. The expression of VEGF is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatin M and tumor necrosis factor alpha. The expression level of VEGF is also different during development and in adults.\u003cbr\u003eThe dimer of VEGF binds to two related tyrosine kinase receptors, namely VEGFR1 (also called Flt-1) and VEGFR2 (Flk-1\/KDR). VEGF can induce both homodimerization and autophosphorylation of the latter. These receptors have seven extracellular immunoglobulin domains and one intracellular separate tyrosine receptor domain. Both vascular endothelial cells and some other non-endothelial cells express VEGF receptors. Although VEGF has the highest affinity with VEGFR1, VEGFR2 is the main factor regulating angiogenesis of VEGF. VEGF165 also binds to the semaporin receptor, Neuropilin-1, thereby promoting complex formation with VEGFR2.\u003cbr\u003eVEGF is known for its involvement in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, and thus regulates the density and volume of blood vessels. But it doesn't work on the pattern of vascularization. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemokine. Postpartum, VEGF maintains the integrity of vascular endothelial cells and is an effective mitogen for large\/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and the female reproductive cycle. In diseased tissues, VEGF promotes vascular permeability. Therefore, VEGF is involved in the metastatic process of tumors through extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control tumor angiogenesis induced by VEGF. The level of VEGF circulating in the body is related to the extent of autoimmune diseases (such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, etc.).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306265675,"sku":"abs510008-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f6bfd547c6e04af9bee254629f4aebbd.jpg?v=1792753468"},{"product_id":"human-egf-elisa-kit","title":"Human EGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentrated dilution buffer in the kit is 10 × mother liquor, and before use, it needs to be diluted to 1 × working liquid with distilled water.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e⑤ Color development solution: According to 100uL per well, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The chromogenic solution removed is for the same day only.\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 250pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (250 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241112\/1e4f67a74dfc43139d3536d892d574fa.png\" alt=\"\" width=\"438\" height=\"260\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241112\/5d55d533c80e4a5aa2127bf869fac4cb.jpg\" alt=\"\" width=\"459\" height=\"357\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human EGF were spiked into cell culture medium samples and the recovery rate was determined. The recovery range is 96-103%, and the average recovery is 99%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human EGF is generally 0.089-0.740 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human EGF protein expressed by sf-21 insect cells.\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of Human EGF, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e100-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e102-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e103-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e100-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant Human EGF protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human EGF. Interference with Human EGF was detected by incorporating 50 ng\/mL of interfering factor into the mid-range recombinant Human EGF control. No significant cross-reactivity or interference was observed. Cross-reacted approximately 2.3% with recombinant Human Pro-EGF.\u003cbr\u003e\u0026lt; tdstyle = \"width: 24.0691%; text-align: center; height: 22px;\" \u0026gt; EGF R\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 54px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eEGF\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 10px;\"\u003eHB-EGF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.53pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human EGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the EGF present in the sample combines with the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman epidermal growth factor ELISA kit, beta-Urogastrone, EGF, epidermal growth factor (beta-Urogastrone), epidermal growth factor, HOMG4, pro-epidermal growth factor, URG, Urogastrone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman EGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman EGF Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman EGF detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe EGF (epidermal growth factor, also known as Urogastrone) precursor is a Group I member of the EGF family of growth factors with a molecular weight of 185kDa. Group I members are molecules that bind and activate the epidermal growth factor receptor (EGFR). EGF family members are synthesized as type I transmembrane (TM) proteins and are proteolyzed to a soluble form. Human epidermal growth factor (EGF) is a fragment in the form of a 1185 amino acid precursor molecule, containing 1010 amino acid extracellular region, 21 amino acid TM region and 153 amino acid cytoplasmic domain. The extracellular domain (ECD) of this precursor has three main structural modules. These include nine BLDLR repeats, one von Willebrand factor A region, and nine EGF-like repeats, and contain the 53 amino acid structure of the mature EGF molecule in the proximal membrane region (amino acids 971 to 1023 of the precursor form). This transmembrane 185kDa isoform (amino acids 21-1023) is found in most body fluids. This process also produces a large number of protein fragments of 40-100kDa, which may be proteolytic degradation products. The production process of 6kDa mature EGF isoforms is unknown. It may originate inside the cell or on the cell surface by hydrolysis of a soluble 160 kDa precursor or a 70 kDa molecular isoform produced after hydrolysis of this precursor by membrane-bound serinase on the cell surface.\u003cbr\u003eNotably, the mature form of EGF, the 185 kDa transmembrane protein, the circulating molecule that has been hydrolyzed but not yet processed, and the 160 kDa precursor molecule are all biologically active. These biological activities are mainly due to the embedding of EGF peptides in these precursor molecules. Modifications of other types of EGF do not have the activity to bind to EGF receptors. There are four possible splice bodies in the expression of EGF protein in the gene encoding EGF, but none of them affect the sequence of mature EGF. Two of these splicers occurred in the extracellular region sequence, deleting amino acids from positions 913 to 953 and amino acids from positions 314 to 355, respectively. The other two splicebodies involve the intracellular region of EGF, replacing amino acids from positions 1125 to 1207 with 12 amino acids and amino acids from positions 1136 to 1207 with 17 amino acids, respectively. The homology of mature human EGF to mouse, rat and pig EGF is 70%, 70% and 85%, respectively. Cells known to express EGF include platelets, cerebral neurons, astrocytes and cerebellar Purkinje cells, Bruner cells (duodenum) and submandibular gland cells, unstained ciliated epithelium, and anterior pituitary cells. EGF has many different physiological effects. At present, the widely recognized function of EGF is mainly based on the binding of EGF receptor-ligand. EGF receptors can also bind to other EGF family proteins, thereby forming heteromultimers and dimerizing with other EGF receptor family members, or associating with other transmembrane proteins such as PDGFR and HGF receptors. In either case, EGF can have an effect on fetal and adult tissues. In the fetus, EGF affects thymocyte growth and differentiation during the double-negative-double-positive stage. During neuronal formation, EGF also appears to stimulate the generation of glial neurons and promote their epithelialization. Finally, it inhibits the maturation of fat cells, thus increasing the preadipocyte count. In adults, EGF can play a role in the milk production process of mammary gland, and can also cause fibroblast mitosis, ECM dissociation and migration, and widely affect various effects related to growth factors.\u003cbr\u003eThe mechanism of activation of EGF receptors, epidermal growth factor receptors (also known as HER1 and ErbB1), is unclear. Current research believes that each EGF molecule and an EGF receptor molecule have two binding sites, which will force the receptor to undergo conformational changes and bind to the second EGF-EGFR complex. This dimer is the EGF receptor with actual function. ErbB2 can also form heterodimers with the EGF receptor, but cannot bind to EGF. This may be because ErbB2 naturally exists as a dimer, which masks its ligand binding site, so that the ligand cannot bind to it. Therefore, it needs to bind an already activated EGF-EGFR complex to form a functional EGF receptor. However, due to the intrinsic electrostatic repulsion, ErbB2 itself cannot form homodimers. EGF can also form heteromultimer with ErbB3: ErbB2 only at high concentrations. The importance of this process is still unknown. Alternatively spliced isoforms of EGFR are present in tumor cells and may be involved in tumorigenesis or sensitize cells to EGF receptor inhibitors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.9pg\/mL-250pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306331211,"sku":"abs510009-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/50373dd8d8f64163b53dfe88fcf8ade8.jpg?v=1792753472"},{"product_id":"human-mmp-9-elisa-kit","title":"Human MMP-9 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Color development solution: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The chromogenic solution removed is for the same day only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240823\/02588be0212b40e5a615fa82a09e78d5.jpg\" alt=\"\" width=\"347\" height=\"270\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels of Human MMP-9 were spiked into cell culture medium samples and the recovery rate was determined. The recoveries ranged from 85 to 104%, with an average recovery of 97%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human MMP-9 is generally 0.156 ng\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human MMP-9 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of Human MMP-9, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 极速赛车开奖直播官网\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Sensitivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;0.079ng\/mL\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Species Reactivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Human\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human MMP-9 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP-9 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Synonym\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Please use within the expiration date of the kit\u0026lt;\/strong\u0026gt;\u0026lt;br \/\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman MMP-9 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman MMP-9 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman MMP-9 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMatrix metalloproteinases (MMPs), also known as matrixins, are members of the zinc-calcium-dependent proteolytic enzyme family, which can break down the extracellular matrix (ECM) and participate in the processing of various molecules in different subcellular environments. MMPs play important functions in numerous physiological processes, such as embryonic development, morphogenesis, reproduction, and tissue remodeling. Also involved in inflammation as well as autoimmune diseases such as arthritis, cancer and cardiovascular diseases. The amount of newly synthesized MMPs is mainly regulated at the transcriptional level, and the proteolytic activity of already existing MMPs is controlled not only by the activity of the zymogen, but also by the inhibition of enzyme activity by endogenous inhibitors, such as α2-macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs).\u003cbr\u003eMMP-9 (also known as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, type V collagenase) is secreted as a proglycosylation enzyme. Activation of the zymogen involves proteolytic removal of the N-terminal precursor region, resulting in the formation of an active enzyme of 82 kDa. Active human MMP-9 has 72% and 74% amino acid sequence homology with mouse and rat MMP-9, respectively. In addition to the zinc binding site, the catalytic domain contains three consecutive fibronectin type II homologous units that bind to gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal heme-like domain. In vitro experiments, after treatment with 4-aminophenyl ethyl acetate (APMA), the enzymogen can not only produce active enzyme, but also produce a C-terminal truncated form with equivalent activity. MMP-9 can degrade components in ECM, especially with very high specificity for denatured collagen (gelatin). MMP-9 also cleaves collagen types III, IV, V, XI, as well as elastin, nestin-1, and vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g., IL-1β, CXCL8\/IL-8, CXCL7, CXCL4, CXCL1, LatentTGF-β, membrane-bound TNF-α, VEGF, and FGFbasic), beta-amyloid, substance P, myelin basic protein. This action may increase or decrease the biological activity of these soluble factors, and may also cause them to be released from association with the ECM. MMP-9 can also trigger signaling pathways through various membrane proteins or induce them to fall off the cell membrane to inhibit signaling pathways (E.g. CD44, E-cadherin, integrin, ICAM-1 and IL-2Rα).\u003cbr\u003eMMP-9 is produced by a variety of normal and transformed cells, including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial cells, and epithelial cells. It affects physiological and pathological angiogenesis and vascular remodeling. Activated neutrophils release the MMP-9 precursor and do not bind to TIMP-1, allowing pro-angiogenic FGF-2 to be released from the ECM. The complex of MMP-9 and TIMP-1 failed to induce the release of FGF-2. Neutrophil-derived MMP-9 can exacerbate the inflammatory response, inducing the release of additional neutrophil MMP-9 by the production of collagen-derived polypeptides. MMP-9 also plays an important role in bone formation and remodeling, methamphetamine-induced behavioral sensitization and response, regulation of neuronal synaptic remodeling, trophoblast invasion during implantation, and activation of serine protease inhibitor α1. MMP-9-mediated shedding of adhesion molecules also has a direct effect on tumor cell invasion.\u003cbr\u003eCirculating levels of MMP-9 are elevated in many inflammatory disorders, including the formation of intraluminal thrombus, atherosclerosis, Crohn's disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy. The ratio of MMP-9 to TIMP-1 was elevated in multiple sclerosis sera and cystic fibrous sputum but decreased in cytomegalovirus-infected sera. The level of free MMP-9 and the level of MMP-9 and lipocalin-2\/NGAL complex were elevated in the urine of patients with ovarian cancer and patients with uterine urethral tract infection, respectively.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306363979,"sku":"abs510010-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d30d0a4d685b4674a16036febbc8f9df.jpg?v=1792753474"},{"product_id":"human-pd-1-elisa-kit","title":"Human PD-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePD-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. You need to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e\n1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength)\u003cbr\u003e\n2. High precision liquid dispenser and disposable tip\u003cbr\u003e\n3. Distilled or deionized water\u003cbr\u003e\n4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\n5. 500mL Measuring cylinder\u003cbr\u003e\u003cbr\u003e\n\n\u003cstrong\u003e2. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\n\u003cstrong\u003e1 Sample collection and storage\u003c\/strong\u003e\u003cbr\u003e\n\u003cstrong\u003eCell culture supernatant\u003c\/strong\u003e: Particulate matter should be removed by centrifugation; Test the sample immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) Dilution.\u003cbr\u003e\n\u003cstrong\u003eSerum:\u003c\/strong\u003e Using serum separation tubes (SST) Collect samples and place samples at room temperature 30 Minutes.\u003cbr\u003e\nCentrifugation 15 Minutes, with a rotation speed of 1000 g。\u003cbr\u003e\nThe serum was removed immediately and tested immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) dilution.\u003cbr\u003e\n\u003cstrong\u003ePlasma:\u003c\/strong\u003e Using EDTA, heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000 g, and detect it immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) dilution.\u003cbr\u003e\n\u003cstrong\u003e2 Preparation work before testing\u003c\/strong\u003e\u003cbr\u003e\nPlease place all reagents and samples at room temperature before use and let them stand 15 Minutes.\u003cbr\u003e\nIt is recommended that all experimental samples and standards be repeatedly tested\u003cbr\u003e\n\u003cstrong\u003e1× Wash solution preparation:\u003c\/strong\u003e The concentrated wash solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Take 10ml Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared.\u003cbr\u003e\n\u003cstrong\u003e1× Buffer preparation for dilution:\u003c\/strong\u003e The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Take 3ml Buffer for concentration and dilution +27mL Distilled water to volume to 30mL。\u003cbr\u003e\nIn actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\n\u003cstrong\u003eAntibody detected:\u003c\/strong\u003e Centrifuge the dry powder to the bottom of the tube and use 110μL Buffer for dilution (1×) Dissolve and let stand at room temperature 5min Later obtained 100× Mother liquor;\u003cbr\u003e\nBefore use, dilute to 1× Working fluid.\u003cbr\u003e\nAccording to the amount per well 100μL Calculate the required volume.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Used 10 Hole, then take 10μL Of 100 Double working concentration of detection antibody, using dilution buffer (1×) Constant volume to 1mL, obtained 1ml Of 1× Detection antibody at working concentration\u003cbr\u003e\n\u003cstrong\u003eSA-HRP：\u003c\/strong\u003e SA-HRP For 40× Mother liquor, use dilution buffer before use (1×) Diluted and formulated 1× Working fluid, the required amount per hole is 100 μL。\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Used 10 Hole, then take 25μL Of 40× Mother liquor +975uL Buffer for dilution (1×) Constant volume to 1mL, obtained 1ml Of 1× Detection antibody at working concentration.\u003cbr\u003e\n\u003cstrong\u003eColor development solution:\u003c\/strong\u003e Per hole 100 μL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light;\u003cbr\u003e\nThe developer removed is for the same day use only.\u003cbr\u003e\n\u003cstrong\u003eStandard:\u003c\/strong\u003e Dilution buffer for lyophilized standards (1×) re-dissolve,\u003cbr\u003e\n\u003cstrong\u003eRe-dissolve Volume 1000μL\u003c\/strong\u003e, obtaining a concentration of 10000pg\/mL Standard mother liquor.\u003cbr\u003e\nGently shake at least 5 Minutes, it is fully dissolved.\u003cbr\u003e\nAdd to each dilution tube 300 μL \u003cstrong\u003eDilution Buffer （1×）\u003c\/strong\u003e。\u003cbr\u003e\nMake serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube.\u003cbr\u003e\nStandard mother liquor without dilution can be used as the highest point of standard curve (10000pg\/mL)，\u003cbr\u003e\n\u003cstrong\u003eDilution Buffer （1×）\u003c\/strong\u003e Can be used as a standard curve zero (0 pg\/mL）。\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20251224\/a8366755e6274cd0ac4efc73842d4a48.png\" alt=\"\" width=\"354\" height=\"202\"\u003e\u003cbr\u003e\n\u003cstrong\u003e3. Experimental operation steps\u003c\/strong\u003e\u003cbr\u003e\n1 Prepare all required reagents and standards;\u003cbr\u003e\n2 Take out the microplate from the sealed bag that has been balanced to room temperature.\u003cbr\u003e\nPlease put the unused slats back into the aluminum foil bag and re-seal;\u003cbr\u003e\n3 Adding to the microplate 300μL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper.\u003cbr\u003e\nPlease use it immediately and do not let the microplate dry;\u003cbr\u003e\n4 Add different concentration standard substances, experimental samples or quality control substances into corresponding wells, and each well 100μL。\u003cbr\u003e\nSeal the reaction wells with plate sealing tape and incubate at room temperature 2 hour;\u003cbr\u003e\n5 Suck off the liquid in the plate, and use a bottle washing machine, a multi-channel plate washer or an automatic plate washer to wash the plate.\u003cbr\u003e\nWashing solution per well 300μL Then the wash liquid in the plate is aspirated off.\u003cbr\u003e\nRepeat Operation 3 Times.\u003cbr\u003e\nTrying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results.\u003cbr\u003e\nAt the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\n6 Adding in each microwell 100μL Detect antibodies.\u003cbr\u003e\nSeal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour;\u003cbr\u003e\n7 Repeat the first 5 Step washing operation;\u003cbr\u003e\n8 Adding in each microwell 100μL SA-HRP, room temperature incubation 20 Minutes.\u003cbr\u003e\nBe careful to avoid light;\u003cbr\u003e\n9 Repeat the first 5 Step washing operation;\u003cbr\u003e\n10 Adding in each microwell 100 μL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light;\u003cbr\u003e\n11 Adding in each microwell 50μL Stop solution, the color of the solution in the well will change from blue to yellow.\u003cbr\u003e\nIf the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\n12 After adding the stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength.\u003cbr\u003e\nIf dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\n13 Calculation results: add the corrected absorbance values of each standard and sample (OD450-OD540\/OD570), average of repeated well readings and then subtract the average zero standard OD Value.\u003cbr\u003e\nUsing computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve.\u003cbr\u003e\nAnother way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis.\u003cbr\u003e\nThis process can generate a data fit that is sufficiently useful but less accurate.\u003cbr\u003e\nIf the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20251224\/ab9ea81638f747c8ade624dc1ec313f3.png\" alt=\"\" width=\"263\" height=\"184\"\u003e\u003cbr\u003e\n\u003cstrong\u003e4. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\n1 Recovery rate\u003cbr\u003e\nSpiked different levels of human in cell culture medium samples PD-1 The recovery rate was determined.\u003cbr\u003e\nThe recoveries range from 85.0-111.9%, the average recovery was in 100.9%。\u003cbr\u003e\nInspired human serum samples with different levels of human PD-1 The recovery rate was determined.\u003cbr\u003e\nThe recoveries range from 99.3-121.1%, the average recovery was in 111.0%。\u003cbr\u003e\n2 Sensitivity\u003cbr\u003e\nPerson PD-1 The lowest measurable dose (MDD） Generally less than 18.9 pg\/mL。\u003cbr\u003e\nThe lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve.\u003cbr\u003e\n3 Correction\u003cbr\u003e\nThis ELISA The kit NS0 Expressed high purity recombinant human PD-1 Corrected by protein.\u003cbr\u003e\n4 Linearity\u003cbr\u003e\n4 Different samples were spiked with high concentrations of human PD-1， Then using a diluent (1×） Dilute the sample to the detection range, Its linearity was determined.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.1035%; margin-left: auto; margin-right: auto; height: 168px;\" border=\"1\" width=\"99%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003eAverage \/ Expected value (%）\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003escope (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e101.1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e89.9 – 111.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e106.0\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e93.1 – 115.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e102.4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e89.0 – 118.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e104.3\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e88.3 – 118.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n5 Specificity,\u003cbr\u003e\nThis ELISA Method can detect natural and recombinant human PD-1 Egg whites.\u003cbr\u003e\nThe following factors were mixed with diluent (1×） Formulated into 100 ng\/mL Concentration to detect human PD-1 Cross-reactivity of.\u003cbr\u003e\nWill 100 ng\/mL Interference factors incorporated into the intermediate range of recombinant human PD-1 In the reference substance, To detect human PD-1 Of interference.\u003cbr\u003e\nNo significant cross-reactivity or interference was observed.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 82.2387%; height: 180px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eRecombinant porcine protein\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eB7-H1\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eCD28\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eCTLA-4\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eICOS\/ Fc Chimer\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003cstrong\u003e5. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\n1 Whiteboard (after color rendering is completed, no color appears)\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.1359%; height: 286px; margin-left: auto; margin-right: auto;\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n2, flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher)\u003cbr\u003e\n\u003ctable style=\"break-inside: avoid; border-collapse: collapse; margin-left: auto; margin-right: auto; height: 164px; width: 80.4555%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine (TMB) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003cstrong\u003e5. Experimental flow chart\u003c\/strong\u003e\u003cbr\u003e\n\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"221\" height=\"447\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human PD-1 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the wells of the enzyme label plate. After incubation, PD-1 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 90.8654%; margin-left: auto; margin-right: auto; height: 345px;\" border=\"1\" width=\"576\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e\u003cstrong\u003e form \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e\u003cstrong\u003e Shelf life of diluted or redissolved reagent after unpacking \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Block \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e The unused slats are placed back in the aluminum foil bag with desiccant and sealed, and placed in 2-8℃ Storage, 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1  Standard \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e2 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e After dissolution, calculate the dosage and divide it into packages, which can be used in -20℃ store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1  Detection antibodies \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e After the concentrated volume is dissolved, it can be in 2-8℃ storage 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e40 Double concentration at 2-8℃ Preservation; 1 Double working concentration is not recommended for storage \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e10× Buffer for concentration and dilution \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" rowspan=\"4\" width=\"144\"\u003e     After opening, 2-8℃ Storage, 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Chromogenic liquid \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Stop Solution \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e20× Concentrated Wash Buffer \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Sealing film \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e3 Sheets \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e Store at room temperature, to avoid contamination, cannot be reused \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eProgrammed death-1 receptor (PD-1), also known as CD279, is a type I transmembrane protein of the CD28 family of immunomodulatory receptors, other members of which include CD28, CTLA-4, ICOS, and BTLA. Its cytoplasmic tail contains two tyrosine residues that make up the tyrosine-based immunoreceptor inhibitory domain (ITIM) and switch domain (ITSM), which are important for mediating PD-1 signal transduction. As a monomeric receptor, PD-1 can interact with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) in a ratio of 1: 1. PD-1 is expressed in activated T cells, B cells, monocytes and dendritic cells, while PD-L1 is expressed in non-hematopoietic cells (such as lung endothelial cells and hepatocytes) in addition to the above cells. The combination of PD-L1 and PD-1 can induce co-inhibitory signals, promoting T cell inactivation, apoptosis, and functional failure. Therefore, PD-1 and PD-L1 interaction is a key regulator of immune response threshold and peripheral immune tolerance. Blocking the interaction of PD-1 and PD-L1 through antibody or gene regulation can accelerate tumor eradication, suggesting the potential of PD-1 as a target for cancer immunotherapy.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Only for scientific research, not for in vitro diagnosis;\u003cbr\u003e2. Please use it within the validity period of the kit;\u003cbr\u003e3. The components of different kits and kits with different batch numbers cannot be mixed;\u003cbr\u003e4. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except for the last step of dilution with diluent;\u003cbr\u003e5. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e6. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e\u003cbr\u003e\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe unopened kit is stored at 2-8 ℃ and has a validity period of one year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306560587,"sku":"abs510015-96T","price":317.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b8db319c21e54dc8942e538b86f8a0e3.jpg?v=1792753495"},{"product_id":"mouse-il-7-elisa-kit","title":"Mouse IL-7 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable tip \u003cbr\u003e3. Distilled or deionized water \u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes at a rotational speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Color development solution: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of chromogenic solution, and protect it from light; The chromogenic solution removed is for the same day only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 2000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 2000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5. The liquid in the plate is sucked off and the plate is washed using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6. Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7. Repeat th 5 Step washing operation; \u003cbr\u003e8. Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9. Repeat th 5 Step washing operation; \u003cbr\u003e10. Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Four-parameter logic using computer software (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241114\/e2e4d02a892946e48b8be03b5a091360.jpg\" alt=\"\" width=\"369\" height=\"286\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: Mouse IL-7 The lowest measurable dose ( MDD ) is generally less than 1.7pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e2. Correction: This ELISA High-purity recombinant kit expressed by Escherichia coli Mouse IL-7 Corrected by protein. \u003cbr\u003e3. Linearity: 6 High concentrations of different samples were incorporated into Mouse IL-7 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e4. Specificity: This ELISA Method can detect natural and recombinant Mouse IL-7 Egg whites. The following factors were mixed with diluent ( 1× ) configured to 50ng\/mL Concentration of to detect the correlation with Mouse IL-7 Cross-reactivity of. Will 50ng\/mL Of interfering factors incorporated into intermediate range recombination Mouse IL-7 In the reference substance, to detect the Mouse IL-7 Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eIL-7 Rα\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eIL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eTSLP\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eHemolysis, storage for too long, incomplete agglutination, contamination by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse IL-7 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-7 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL7, IL-7, IL-7interleukin-7, interleukin 7, Lymphopoietin-1, PBGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-7 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eMouse IL-7 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eMouse IL-7 Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-7 (Interleukin-7) is a cytokine that plays an important role in lymphocyte differentiation, proliferation and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestine. It signals through a receptor complex consisting of IL-7R alpha\/CD127 and Common γ Chain. γc is also a subunit of the IL-2,-4,-9,-15 and-21 receptors. IL-7 helps to maintain all primitive and memory T cells. Furthermore, IL-7 is required for T cell-dendritic cell interactions. In mice, IL-7 activation of IL-7Ralpha is essential for the development of both T cells and B cell lineages, whereas in humans T cells are not required, whereas B cell development is required.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311082571,"sku":"abs520020-96T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-il-2-elisa-kit","title":"Mouse IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\u003cbr\u003e5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/9297927b5c1b4d6dbdde6fd6fcaad53d.png\" alt=\"\" width=\"433\" height=\"245\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240201\/84b805a141c641e18449b8ec36ac948f.jpg\" alt=\"\" width=\"301\" height=\"235\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery rate: Different levels of Mouse IL-2 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 84 to 107%, with an average recovery of 95%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: Mouse IL - 2 the minimum detectable dose (MDD) is generally less than 3.5 pg\/mL. Minimum measurable value is according to the 20 standard curve zero absorbance value plus two times the standard deviation of average calculated corresponding to the concentration.\u003cbr\u003e\u003cbr\u003e3. Correction: the ELISA kit by e. coli expressed high purity recombinant Mouse IL - 2 protein correction.\u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse IL-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e96-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e97-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e99-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: Both native and recombinant Mouse IL-2 protein could be detected by this ELISA. The following factors were diluted (1×) Configure to a concentration of 50ng\/mL to detect cross-reactivity with Mouse IL-2. To detect interference with Mouse IL-2, 50ng\/mL of interfering factor was incorporated into the intermediate range of recombinant Mouse IL-2 controls. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e IL-9\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e IL-15\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rβ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车5码公式\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-2 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-2 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. \"Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor.\" Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25\/IL-2Rα+Treg cells. It is important to note that now IL - 2 is considered successful induction of CD8 + memory cells necessary for memory response. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. The two subunits can be combined with ligands, called IL - 2 r alpha and beta IL - 2 r. IL-2Rα is 55kDa and binds IL-2 with low affinity. IL - 2 beta for 75 kda, is part of the IL - 15 receptors, moderate affinity with IL - 2. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). Gamma c and IL - 4, 7, 9, 15-21 and Shared receptors. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311180875,"sku":"abs520002-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/83ff000cd49749aaa9f0572e7394cfe2.jpg?v=1792753496"},{"product_id":"mouse-il-4-elisa-kit","title":"Mouse IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240320\/990f0778e326410e9897ab9366ec1de1.jpg\" alt=\"\" width=\"428\" height=\"333\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3, kit parameter \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of mouse IL-4 were incorporated into the cell culture medium samples and the recovery rate was determined. Recovery range in 75-112%, the average recovery rate at 90%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-4 in mice was generally less than 2.3pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 4 protein in mice. \u003cbr\u003e4. Linear: four different samples with high levels of IL - 4 in mice, and then use thinner (1 \u0026amp; times) Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.2527%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e96-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e109\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-116\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1：16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e98-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse IL-4 protein. The following factors in diluent (1 \u0026amp; times) The concentration of the mixture 50 ng\/mL to detect and IL - 4 cross reaction in mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 4 reference substance in the restructuring of the mice, the interference of IL - 4 to test in mice. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 41.7154%; height: 82px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human proteins\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4R\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cstrong\u003e4, common problem resolution\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车开奖官网 is a government-run official platform that provides timely and accurate lottery results. Users can check the latest winning numbers, prize distributions, and historical data to stay informed. The website ensures fairness and transparency in all lottery operations, offering a trusted source for enthusiasts. Always play responsibly and within legal boundaries.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Sensitivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;2.75pg\/mL\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-4 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the well of microplate. After incubation, IL-4 present in the sample combined with solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-4 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-4 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-4 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-4 (IL-4), also known as B cell stimulating factor 1, is a monomeric cytokine of about 18-20 kda that has pleiotropic effects in immune responses. The precursor of mouse IL-4 is 140 amino acids, including a 20-amino acid signal peptide and a 120-amino acid mature chain. IL-4 contains three N-linked glycosylation potential sites, three intra-chain disulphide bonds, and its structure is a bundled tetra-α-spirochetes. Similar to human IL-4, mouse IL-4 has a small selective splicer. \"Unlike human IL-4, the expression of this spliceosome is thought to be low and of little significance.\" The amino acid sequence of mature mouse IL-4 shared 43% and 63% identity with human and rat IL-4, respectively. Studies have shown that the activities of human, mouse and rat IL-4 are species specific. IL - 4 by toward Th2 CD4 + T cells, basophils and mast cells and NKT cells, gamma delta T cells with eosinophilic cells expressed. \u003cbr\u003eIL - 4 has played a key role in type 2 immune reaction, can promote the Th2 differentiation and B cell secretory immunoglobulin G1 and immunoglobulin E subtypes. IL-4 causes Th2 differentiation by binding to type I or type II receptor complexes containing IL-4 receptor alpha subunits coupled to the γ chain or IL13Rα1, respectively. IL - 4 by cell surface receptors of different dimers, activate JAK family of protein complex amino acid kinase, cause its cytoplasm C phosphorylation. This triggers the recruitment and phosphorylation of STAT6. The phosphorylation and conformational changes of STAT6 lead to dimerization, nuclear translocation, binding and transcriptional activation of STAT6 target genes, including IL-4, IL-5, IL-13, and Th2-specific cytokines GATA3 and c-Maf. On the function, IL - 4 B cell proliferation, survival, and promote mice immune globulin class into IgG1 and IgE, promote the mast cells, eosinophils, and basophils start and chemokines, promote Th2 phenotype naive CD4 + cells, and promote the epithelial cell proliferation and activation. IL-4 is also an important cytokine in tumor immunology. In early experiments in mice, the researchers found that IL-4 had potent anti-tumor abilities. Mice reject tumors expressing IL-4 and develop long-term antitumor immunity. This may be due to the antiangiogenic effect of IL-4 or its ability to activate, select for CD8+T cells. \"Paradoxically, emerging evidence suggests that IL-4 is a tumor-promoting molecule, and therefore, IL-4 is a reverse-acting cytokine.\" \"A possible explanation is that IL-4 induces upregulation of antiapoptotic molecules in tumor cells and downregulation of cytolytic molecules in CD8+T cells.\" In addition, tumor-produced IL-4 is also thought to act on local tumor-associated macrophages (TAMs) to induce the secretion of cathepsins that promote cell migration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311246411,"sku":"abs520003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/87e652a2480d4f0b85b4ca6747b6bdf2.jpg?v=1792753498"},{"product_id":"mouse-il-6-elisa-kit","title":"Mouse IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e①1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240109\/9ef19a3c0d9b4bd496544968c6147c31.jpg\" alt=\"\" width=\"272\" height=\"212\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Mouse IL-6 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 70 to 114%, with an average recovery of 91%. \u003cbr\u003e\u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of Mouse IL-6 was generally less than 1.8pg\/mL. The lowest detectable value of mouse IL-6 was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with high purity recombinant Mouse IL-6 protein expressed in E. coli. \u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse IL-6, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003ctable style=\"border-collapse: collapse; width: 56.9582%; height: 209px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e109\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e102-112\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 12px;\"\u003e1：16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e117\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 12px;\"\u003e112-120\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA can detect native and recombinant Mouse IL-6 protein. The following factors in diluent mixture 50 ng\/mL (1 x) to detect the concentration and Mouse IL - 6 cross reaction. Interference with Mouse IL-6 was tested by incorporating 50ng\/mL of the interfering factor into the intermediate range of recombinant Mouse IL-6 controls. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 47.5817%; height: 32px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.49pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-6 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-6 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-6 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-6 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-6 (IL-6) is a multifunctional cytokine with α-helical structure, 22-28kD phosphorylation and different degrees of glycosylation. It plays an important role in the acute phase of disease response, inflammation, hematopoiesis, bone metabolism and cancer progression. Mature mouse IL-6 has 187 amino acids and is 42% and 85% identical to human and rat IL-6, respectively. Alternative splicing within IL-6 results in a variety of isoforms, some of which exhibit antagonistic properties. Expression of IL - 6 cells is known, \"These include CD8+T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, and neutrophils. , monocytes, eosinophils, colon epithelial cells, B1B cells and islet beta cells. The production of IL - 6 is usually in the sugar cortical hormone, catecholamine and under the control of secondary sexual steroids, generally associated with cell activation. The level of IL-6 in normal human blood is in the range of 1pg\/mL. It is slightly increased in the menstrual period, moderately increased in the middle and late stage of some cancers, and significantly increased after major surgery. \u003cbr\u003eIL-6 elicits cell signaling through a cell surface receptor, which is a heterodimeric complex consisting of a ligand-binding subunit (the IL-6 receptor) and a signal transduction subunit gp130. IL-6 binds to the IL-6 receptor, triggering the binding of IL-6 receptor to gp130 and the dimerization of gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble IL - 6 receptor is produced by alternative splicing and protease cutting. \"Through a trans-signaling mechanism, the combination of soluble IL-6 and IL-6 receptor triggers a response in cells that lack the IL-6 receptor on the surface but express gp130.\" Expression of the IL-6 receptor is largely restricted to hepatocytes, monocytes, lymphocytes, and resting lymphocytes, and since the gp130 molecule is expressed very broadly, trans-signaling enables a broader range of cell types to respond to IL-6. \"Soluble gp130 spliceosome prevented IL-6\/IL-6R trans-signaling, but not signaling by other cytokines using gp130 as a co-receptor.\" \u003cbr\u003eThe acute inflammatory response induced by IL-6, together with tumor necrosis factor-α (TNF-α) and IL-1, plays an almost unique role in fever and acute liver inflammation. It also plays an important role in the transformation from acute inflammation to acquired immunity or chronic inflammatory diseases. IL-6 dysregulation can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving IL-6 trans-signaling. In the presence of transforming growth factor TGF-β, IL-6 plays an important role in the differentiation of Th17 inflammatory cells from naive T cells. IL-6 regulates bone resorption by promoting the activity of Th17 inflammatory cells, which is the main factor causing inflammatory joint damage in rheumatoid arthritis. IL-6 is involved in the formation and destabilization of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects, such as secretion of IL-6 by skeletal muscle during physical exercise, promoting hematopoiesis as a growth factor for hematopoietic stem cells, inducing the maturation of B cells into plasma cells, and immortalization of multiple myeloma cells. Also promote IL - 6, but may not start the other inflammation associated with cancer, such as colitis associated cancer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311279179,"sku":"abs520004-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/95bc6c59708e4c14b8e6c76753199553.jpg?v=1792753504"},{"product_id":"mouse-vegf-elisa-kit","title":"Mouse VEGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL). \u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/3518f8f0e7ba44aba5337ea9784cf5c2.png\" alt=\"\" width=\"385\" height=\"218\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Ready for all the needed reagent and standard; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/51b42183e55442c5b538655dcd06491f.jpg\" alt=\"\" width=\"241\" height=\"187\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Mouse VEGF were incorporated into the cell culture medium samples and the recovery rate was determined. Recovery range in 82-110%, the average recovery rate at 94%. Sensitivity: \u003cbr\u003e\u003cbr\u003e2. The minimum measurable Mouse VEGF dose (MDD) is generally less than 1.8 pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with high purity recombinant Mouse VEGF protein expressed by Sf21. \u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse VEGF followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range. \u003cbr\u003e\u003cbr\u003e5. Specificity: both native and recombinant Mouse VEGF protein could be detected by this ELISA. The following factors in diluent (1 \u0026amp; times) Configure to a concentration of 50ng\/mL to detect cross-reactivity with mouse VEGF. Interference with mouse VEGF was detected by incorporating 50ng\/mL of the interfering factor into the intermediate range of recombinant mouse VEGF controls. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.9244%; height: 120px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF R2\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF115\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF-B167\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF165\/pIGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF-B186\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003eVEGF-D\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4, common problem resolution\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specificity of anti VEGF antibody in mice pre package is on the high affinity enzyme label plate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the VEGF present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse VEGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse VEGF standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse VEGF detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is A potent regulator of neovascularization and angiogenesis in fetuses and adults. Vascular endothelial growth factor is PDGF family, the family proteins there are eight characteristics of conservative and cystine nodes through a reverse parallel disulfide bond dimers (4). After selective splicing, VEGF spliceosome has different amino acid lengths. VEGF120, VEGF164, VEGF188 in mice; Human VEGF VEGF121, VEGF145, VEGF164, VEGF183, VEGF189 and VEGF206. VEGF165 was the most expressed and active isoform in human, followed by VEGF121 and VEGF89. The same may be true of mice. With the exception of VEGF120 and VEGF121, the other isoforms of VEGF contain the basic heparin-binding domain and do not diffuse freely. Mice VEGF164 and corresponding protein amino acid 97% homology of rats; The homology with human or porcine VEGF was 89%, with bovine VEGF 88%, and with cat, horse, and dog VEGF 90%. VEGF is expressed in a variety of cells and tissues, including skeletal muscle and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34+ stem cells, endothelial cells, cellulose cells, vascular smooth muscle cells, and so on. VEGF expression is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatinM and tumor necrosis factor-α. Expression of VEGF isoforms also varies during development and in adults. \"The dimer of \u003cbr\u003eVEGF binds to two related tyrosine kinase receptors, VEGFR1 (also called FLt-1) and VEGFR2 (Flk-1\/KDR).\" Binding of VEGF to its receptor induces homodimerization and autophosphorylation of the latter. These receptors possess seven extracellular immunoglobulin-like domains. Vascular endothelial cells and some non-endothelial cells have VEGF receptor expression. \"Although VEGF has the highest affinity for VEGFR1, VEGFR2 is the major factor regulating the angiogenic activity of VEGF.\" VEGF165 also binds to the semaphoring receptor and neuropilin-1, thereby promoting the complex formation with VEGFR2. \u003cbr\u003eVEGF is famous for its participation in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, thereby regulating the density and volume of blood vessels, but it does not play a role in the pattern of blood vessel formation. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemotactic factor. In the postpartum period, VEGF maintains the integrity of vascular endothelial cells and is a potent mitogen for large\/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and in the female reproductive cycle. In disease tissues, VEGF promote the permeability of blood vessels. Thus, VEGF is involved in the metastatic process of tumors by promoting extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control VEGF-induced tumor angiogenesis. The level of circulating VEGF correlates with the severity of autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311508555,"sku":"abs520008-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/705eaefdf45a44128d4c3dbd11f3772d.jpg?v=1792753520"},{"product_id":"porcine-il-6-elisa-kit","title":"Porcine IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.1pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti-porcine IL-6 antibody was pre coated on a high affinity microplate. Standards, samples to be tested and biotinylated detection antibodies are added into the wells of enzyme plate. After incubation, IL-6 in the samples combines with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003epre coated 96 well plate, standard, anti-porcine IL-6 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8  Pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e125 - 8000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e70-114%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191227\/10e0016ad35d4564a34397548ab2faa4.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin-6 (IL-6)Is a α Helical structure, 22-28kd phosphorylation and different degrees of glycosylation of multifunctional cytokines, which play an important role in the acute phase of disease reaction, inflammation, hematopoiesis, bone metabolism and cancer deterioration. Mature mouse IL-6 has 187 amino acids and has 42% and 85% homology with human and rat IL-6, respectively. The alternative splicing of IL-6 generates a variety of isoforms, some of which show antagonistic properties. Cells known to express IL-6 include cd8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cellsFetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islets beta; Cells. IL-6 production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids, and is generally associated with cell activation. IL-6 in the blood of normal people is in the range of 1 pg\/ml, which increases slightly during menstruation, moderately in the middle and late stages of some cancers, and significantly after major surgery.\u003cbr\u003e\u003cbr\u003eIL-6 triggers cell signals through cell surface receptors, which are heterodimeric complexes composed of ligand binding subunit (IL-6 receptor) and signal transduction subunit gp130. IL-6 binds to the IL-6 receptor, triggering the binding of IL-6 receptor to gp130 and the dimerization of gp130. Gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptor is produced by alternative splicing and protease cleavage. Through the trans signal transduction mechanism, the complex of soluble IL-6 and IL-6 receptor can trigger the response of cells lacking IL-6 receptor on the surface but expressing gp130. The expression of IL-6 receptor is mainly limited to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Due to the wide expression of gp130 molecule, trans signal transduction realizes the response of a wider range of cell types to IL-6. The soluble gp130 spliceosome prevented the trans signal transduction of il-6\/il-6r, but could not prevent the signal transduction of other cytokines using gp130 molecule as a co receptor.\u003cbr\u003e\u003cbr\u003eIL-6 and tumor necrosis factor alpha; (tnf-α)Together with IL-1, the acute inflammatory response caused by IL-1 plays an almost unique role in fever and liver acute inflammatory response. It also plays an important role in the transformation from acute inflammation to acquired immunity or chronic inflammatory diseases. Dysregulation of IL-6 can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis and sepsis, often involving the trans signal transduction of IL-6. In transforming growth factor TGF - β In the presence of IL-6, IL-6 plays an important role in the differentiation of naive T cell phase Th17 inflammatory cells. IL-6 regulates bone resorption and promotes the activity of Th17 inflammatory cells, which is the main factor causing inflammatory joint injury in rheumatoid arthritis. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects. For example, skeletal muscle secretes IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes hematopoiesis, induces B cells to mature into plasma cells, and the immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation related cancers, such as colitis related cancers.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313441867,"sku":"abs540003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/835d962b9d3648ca9f4cb34af1f7e82f.jpg?v=1792753355"},{"product_id":"rat-il-2-elisa-kit","title":"Rat IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL). \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"399\" height=\"224\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240313\/df8f669701d44968a318ad6bb3e01849.jpg\" alt=\"\" width=\"300\" height=\"234\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of rat IL-2 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 84 to 107%, with an average recovery of 95%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-2 in rats was generally less than 3.5pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Calibration: The ELISA kit was calibrated with recombinant rat IL-2 protein expressed in E. coli. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of rat IL-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e95-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e96-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e97-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e99-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect both native and recombinant rat IL-2 protein.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 7.04137%; height极速赛车稳赚技巧\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-rat IL-2 antibody was precoated on a high-affinity microplate. Enzyme label plate hole with standard substance, the sample under test and biotinylated antibodies detection, after incubation, IL - 2 that exist in the sample combined with solid phase antibody and detection antibody, immune complex. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eRat  IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eRat  IL-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eRat  IL-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. \"Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor.\" Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25\/IL-2Rα+Treg cells. Notably, IL-2 is now recognized as essential for successful induction of recall responses in CD8+ memory cells. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. Two of these subunits can bind ligands and are termed IL-2Rα and IL-2Rβ. IL-2Rα is 55kDa and binds IL-2 with low affinity. Il-1β, 75kDa, is also a component of the IL-15 receptor and binds IL-2 with moderate affinity. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). γc shares receptors with IL-4, -7, -9, -15 and -21. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313507403,"sku":"abs530005-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7965d16116ec4bd894b64ab2348f3c20.jpg?v=1792753310"},{"product_id":"rat-il-4-elisa-kit","title":"Rat IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.91pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-rat IL-4 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-4 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eS\u003cspan style=\"color: #000000;\"\u003eample \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e  2.3 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1,000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e 75-112%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/57eea580c2d3435db8e80b0ee9cfefe3.png\" alt=\"\" width=\"600\" height=\"405\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: IL-4\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIl-4 is a secretory protein of 129 amino acids.Il-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils.By acting on a variety of cell types, IL-4 regulates a variety of immune responses. Il-4 is involved in several B cell activation processes and induces class II MHC molecules to be expressed in dormant B cells.It is also a common irritant in DNA synthesis. Il-4 can enhance the secretion and cell surface expression of IgE and IgG1.It also regulates the expression of low affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes.Il-4 positively regulates the expression of IL31RA in macrophages.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical hints: \u003c\/strong\u003eFor research use only, not for in vitro diagnosis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313572939,"sku":"abs530006-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d94654b6a2174d47a3631488a6cbab93.jpg?v=1792753308"},{"product_id":"rat-il-6-elisa-kit","title":"Rat IL-6 ELISA  Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable tip \u003cbr\u003e3. Distilled or deionized water \u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 8000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 8000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240111\/b1361d91d1f94ed28d56f04effb5904e.png\" alt=\"\" width=\"434\" height=\"252\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5. Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6. Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7. Repeat th 5 Step washing operation; \u003cbr\u003e8. Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9. Repeat th 5 Step washing operation; \u003cbr\u003e10. Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Four-parameter logic using computer software (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240111\/e6f5ca75fa494b62b30c679050174f04.jpg\" alt=\"\" width=\"343\" height=\"267\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels were spiked into cell culture medium samples Rat IL-6 The recovery rate was determined. The recoveries range from 82-110% , the average recovery was in 94% 。 \u003cbr\u003e2. Sensitivity: Rat IL-6 The lowest measurable dose ( MDD ) is generally less than 1.8pg\/mL 。 The lowest measurable value is determined according to 20 \u003cbr\u003e3. Linearity: 4 High concentrations of different samples were incorporated into Rat IL-6 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e4. ELISA Method can detect natural and recombinant Rat IL-6 1× ) formulated into 50ng\/mL Concentration of to detect the correlation with Rat IL-6 Cross-reactivity of. Will 50ng\/mL Rat IL-6 In the reference substance, to detect the Rat IL-6 \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 69.8827%; height: 76px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant rat protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eCNTF\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant porcine protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. No color development after substrate addition (blank well)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible causes\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, extend the incubation and substrate development times.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eDuring plate washing, the soak time was too long, the number of wash cycles was excessive, or the wash force was too strong.\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eFollow the instructions for use; do not dilute arbitrarily.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height极 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine (TMB)\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eFailing to change the pipette tip between samples, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 极 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eIdentify the suspected infectious agent and apply the appropriate handling procedures\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat IL-6 antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell stimulation factor-2, B-cell differentiation factor, BSF2, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, IL-6B-cell stimulation factor 2, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A, Rat interleukin 6 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px; text-align: center;\"\u003eRat IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px; text-align: center;\"\u003eRat IL-6 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px; text-align: center;\"\u003eRat IL-6 Detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px; text-align: center;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px; text-align: center;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px; text-align: center;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px; text-align: center;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px; text-align: center;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px; text-align: center;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Interleukin -6  IL-6 ) is a system with α A spiral structure, 22-28kDa It plays an important role in the acute phase of disease, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. A mature person IL-6 have 183 Amino acids, with mice and rats IL-6 have 41% Homology of. IL-6 Internal alternative splicing results in a variety of isomers, some of which exhibit antagonistic properties. Known expression IL-6 The cells comprise CD8+T Cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical nerve cells, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B  β Cells. IL-6 The production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids and is generally associated with cellular activation. In normal human blood IL-6 In 1pg\/mL Within the range, there is a slight increase in menstrual period, a moderate increase in some cancers, and a significant increase after surgery. \u003cbr\u003eIL-6 Cellular signaling is initiated by a cell surface heterodimeric receptor complex consisting of a ligand-binding subunit \t （ IL-6 Receptor) and a signal transduction subunit gp130 Composition. IL-6 Binding to IL-6  IL-6 Receptor with gp130 Combination of and gp130 Dimerization of. gp130 Also CLC  CNTF 、 CT-1 、 IL-11  IL-27 、 LIF And OSM  IL-6 Receptors are produced by alternative splicing and proteolysis. Through a trans-signaling mechanism, soluble IL-6  IL-6 Receptor complexes can trigger surface deficiency IL-6  gp130 Response of cells. IL-6  gp130 Molecular expression is very broad, and trans-signaling enables a wider range of cell type pairs IL-6 Response of. Soluble gp130 The spliceosome prevents IL-6\/IL-6R Trans-signal transduction, but does not prevent other cytokine utilization gp130 Signal transduction of molecules as co-receptors. \u003cbr\u003eIL-6 With tumor necrosis factor ( TNF ) and IL-1 The acute inflammatory reaction caused by it plays an almost unique role in fever and acute inflammatory reaction of the liver, and it also plays an important role in the transformation of acute inflammation into acquired immunity or chronic inflammatory diseases. IL-6 Disorders contribute to chronic inflammation in cases such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving IL-6  TGF- Under the conditions of existence, IL-6 In the naive type T Cells to Th17 It plays an important role in the process of inflammatory cell differentiation. IL-6 Modulates bone resorption, and by promoting Th17 The active component of inflammatory cells is the main factor causing inflammatory joint damage in rheumatoid arthritis I 。 L-6 Contributes to the formation and instability of atherosclerotic plaque. but IL-6 It also has anti-inflammatory effects, such as skeletal muscle secretion during physical exercise IL-6 。 It acts as a hematopoietic stem cell growth factor to promote hematopoiesis, induction B The cells mature into plasma cells, immortalized multiple myeloma cells. IL-6 It also promotes but initiates the development of other inflammation-related cancers, such as enteritis-related cancers. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, store at 2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e125pg\/mL-8000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313704011,"sku":"abs530003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a58cf7bed63649579d16b1ae2c1666cd.jpg?v=1792756807"},{"product_id":"human-il-1-elisa-kit","title":"Human IL-1β ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 250pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (250 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/866b0454d6af4c8c84cf41fb0a2de971.png\" alt=\"\" width=\"438\" height=\"260\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/5db783ade218491dbddda6039df89d31.jpg\" alt=\"\" width=\"329\" height=\"255\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human IL-1β were spiked into cell culture medium samples and the recovery rate was determined. The recoveries ranged from 85 to 107%, with an average recovery of 97%.\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human IL-1β is generally less than 1pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human IL-1β protein expressed by E. coli.\u003cbr\u003e4. Linearity: Five different samples were spiked with high concentrations of Human IL-1β, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e101-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e105-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant Human IL-1β protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-1β. Interference with Human IL-1β was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant Human IL-1β control. No significant cross-reactivity or interference was observed. Recombinant human IL-1β precursor (aa1-269) showed 3.8% cross-reactivity at a concentration of 125 pg\/mL and 6.3% cross-reactivity at a concentration of 62.5 pg\/mL of recombinant rat IL-1β.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1096%; height: 54px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u0026lt; td style = \"text-align: center; height: 22px; width:24.0666%; \"\u0026gt; Recombinant human protein\u003ctd style=\"text-align: center; width: 24.0666%; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant rat protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant porcine protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eIL-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e5. Experimental flow chart\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-1β antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-1β present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman IL-1β ELISA kit, catabolin, IL1 beta, IL-1 beta, IL-1, IL1B, IL-1b, IL1-beta, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1 beta, Human interleukin 1β Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 92.6054%; margin: 0px auto; break-inside: avoid; height: 391px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-1β Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-1β Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-1β detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 158px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 43px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 43px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 32px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 32px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 30px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 30px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 30px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMembers of the interleukin-1 (IL-1) protein family include classical IL-1α and IL-1β. IL-1 receptor antagonists (IL-1RA), IL-18, IL-33 and IL-1F5-F10, IL-1α and IL-1β have the same cell surface binding receptors and form the same biological function. Unstimulated cells of healthy humans do not produce IL-1, with the exception of skin keratinocytes, some epithelial cells, and some cells of the central nervous system. However, in response to inflammatory factors, infection, or microbial endotoxins, IL-1 expression in macrophages and other types of cells increases dramatically. IL-1β plays an important role in the physiological processes of immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, GH\/IGF-1. A variety of diseases are associated with dysregulated or delayed expression of IL-1, including diseases related to sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and senile degeneration.\u003cbr\u003eIL-1α and IL-1β are structurally related polypeptides with approximately 25% homology at the amino acid level. Both are first synthesized as a 31 kDa precursor and then cleaved to a mature protein of approximately 17.5 kDa. Precursor cleavage of IL-1β by Caspase-1\/ICE is a critical step in the inflammatory response. Although neither IL-1α nor IL-1β contain a typical hydrophobic signal peptide, there is evidence that they can be secreted extracellularly via non-classical pathways. The untreated IL-1α fraction may be displayed on the cell membrane and may retain biological activity. Unlike IL-1β precursors, IL-1β precursors have little or no biological activity compared to mature IL-1β. Both the IL-1β precursor and mature IL-1β are transported extracellularly.\u003cbr\u003eIL-1α and IL-1β exert their effects by binding to IL-1RA through immunoglobulin superfamily receptors. The 80 kDa type I transmembrane receptor (IL-1RI) is expressed in a variety of cells, including T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes. A 68 kDa type II transmembrane receptor (IL-1RII) is expressed in B cells, neutrophils, and myeloid cells. The extracellular regions of IL-1RI and IL-1RII share 28% homology. But other regions are significantly different: the intracellular domain of type II receptors is only 29 amino acids, while the intracellular domain of type I receptors is 213 amino acids. IL-1RII does not appear to respond to IL-1 signaling and functions as a decoy receptor thereby attenuating the effects of IL-1. The interaction of IL-1 receptor ligand protein (IL-1RAcP) with IL-1RI is required for IL-1RI signaling. IL-1RA is a secreted molecule and a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII are present in human plasma, joint fluid, and conditioned media of several cell lines. In addition, vaccinia virus encodes an IL-1 binding protein similar to soluble IL-1RII.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent (1 ×) in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.9pg\/mL-250pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518143766603,"sku":"abs510002-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ba19935caf91421990145656f5cb12d5.jpg?v=1792753457"},{"product_id":"human-il-8-cxcl8-elisa-kit","title":"Human IL-8\/CXCL8 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e⑤ Color development solution: According to 100uL per well, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The chromogenic solution removed is for the same day only.\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240719\/d9207aaf48b54205a6350f3f94bebee0.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240719\/e882243a86b64e2dbe603a4482b47a5b.jpg\" alt=\"\" width=\"423\" height=\"328\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels of human IL-8\/CXCL8 were spiked into cell culture medium samples, and the recovery rate was determined. The recoveries ranged from 96 to 118%, with an average recovery of 108%.\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of human IL-8\/CXCL8 is generally less than 7.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant human IL-8\/CXCL8 protein expressed by E. coli.\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of human IL-8\/CXCL8, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 61.9296%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.6531%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 27.5855%; text-align: center; height: 22px;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 15.7462%; text-align: center;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.6531%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 27.5855%; height: 22px;\"\u003e114\u003c\/td\u003e\n\u003ctd style=\"width: 15.7462%;\"\u003e105-125\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.6531%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 27.5855%; height: 22px;\"\u003e114\u003c\/td\u003e\n\u003ctd style=\"width: 15.7462%;\"\u003e105-122\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.6531%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 27.5855%; height: 22px;\"\u003e112\u003c\/td\u003e\n\u003ctd style=\"width: 15.7462%;\"\u003e104-124\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 19.6531%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 27.5855%; height: 10px;\"\u003e108\u003c\/td\u003e\n\u003ctd style=\"width: 15.7462%;\"\u003e102-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e5. Specificity: This ELISA method can detect natural and recombinant human IL-8\/CXCL8 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IL-8\/CXCL8. Interference with human IL-8\/CXCL8 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IL-8\/CXCL8 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 110px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height极 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eGRO α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eKC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eGRO β\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eGRO Gamma\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eI-309\u003c\/td\u003e\n\u003ctd style=\"width: 极48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIP-10\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eMCP-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eMCP-3\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"极width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-8 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-8 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP1, GCP-1TSG-1, IL8, IL-8, interleukin 8, K60, LAI, LECT, MDNCF, MDNCFb-ENAP, member 8, MONAPGCP1, NAP1, NAP-1NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-8\/CXCL8 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-8\/CXCL8 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-8\/CXCL8 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-8 (IL-8, also known as GCP-1, NAP-1, and CXCL8) is an 8-9kDa chemokine belonging to the α-type or CXC family that binds to heparin. So far, a total of 15 human CXC family proteins have been found, ranging in size from 8 to 12kDa. Most of them are located on human chromosome 4, all have a typical triple β-sheet layer\/an α-helix structure, and most of them show Glu-Leu-Arg tripeptide motif at the N-terminus. Human IL-8 was first synthesized as a 99 amino acid precursor containing a 20 amino acid signal sequence and a 79 amino acid mature region. It can be circulated in vivo as monomers, homodimers, and heterodimers formed with CXCL4\/PF4. IL-8 monomers are considered to be the most biologically active, whereas heterodimers may enhance the activity of PF4. At the amino acid level, mature human IL-8 has 65% and 70% homology to pigs and dogs, respectively. In rodents, the corresponding gene for IL-8 has not been found. A variety of IL-8 isoforms were generated by alternative splicing and differential proteolysis. Alternative splicing occurs at the C-terminus, where there is an 11 amino acid substitution (at aa # 92-99). Protease hydrolysis may be a cell-specific event that produces a truncation at the N-terminus of IL-8. For example, fibroblasts and vascular endothelial cells cleave amino acids 21 and 22 to form IL-8, while monocytes and lymphocytes cleave amino acids 21-25 to produce IL-8. These short IL-8s generally have higher biological activity, especially against the CXCR1IL-8 receptor. Approximately 15% of IL-8 is citrullinated at the precursor Arg27 position, which increases the half-life of IL-8 and promotes leukocytosis. Many types of cells secrete IL-8, including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II large alveolar cells, and endothelial cells.\u003cbr\u003eThere are two IL-8 receptors, both of which belong to G protein-coupled receptor proteins: CXCR1\/IL-8RA and CXCR2\/IL-8RB, and the amino acid sequences between them share 77% homology. CXCR1 has a molecular weight of 45-50 kDa and is almost entirely exclusive to IL-8; CXCR2 has a molecular weight of 35-40 kDa and is common to all CXC chemokines. CXCR1 and CXCR2, respectively, form constitutive homodimers, which appear to be their functional configuration. Heterodimers also form when expressed in the same cell; But when it binds to IL-8, it will be disintegrated. CXCR2 responds to low concentrations of IL-8 and is mainly related to chemotaxis and MMP-9 release. In contrast, CXCR1 responds to high concentrations of IL-8 and is associated with respiratory bursts and activation of phospholipase D2. Therefore, in neutrophils CXCR2 is thought to direct the migration of neutrophils to the site of inflammation and then elicit CXCR1-mediated antibacterial activity.\u003cbr\u003eThe best known function of IL-8 is its pro-inflammatory effect in immune cells. Essentially, IL-8 is secreted by a variety of cell types exposed to inflammatory stimulatory factors. For monocytes\/macrophages, microbial exposure causes the release of IL-8; Subsequently, CXCR2-mediated chemotaxis migrates neutrophils to the site of antigen attack, accompanied by the activation and initiation of antibacterial activity in the next step. IL-8 can enhance the effect of M-CSF in bone marrow, thereby causing the maturation and release of granulocytes. These two functions of IL-8 complement each other. It has been reported that IL-8 has angiogenic function on tumor-associated endothelial cells. At this time, tumor-derived IL-8 can activate CXCR1 and CXCR2 in vascular endothelial cells in a paracrine manner. Both CXCR1 and CXCR2 are associated with the PI3-K\/Akt and RasGTP signaling pathways and are involved in cell survival and proliferation. In addition, IL-8 positively regulates VEGFR2 and EGFR, which belong to receptor tyrosine kinases that mediate cell growth and migration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518143864907,"sku":"abs510004-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/cb0e6a5f418c4d44a5d8d90273f84607.jpg?v=1792753459"},{"product_id":"human-il-elisa-kit","title":"Human IL-10 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Color development solution: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The chromogenic solution removed is for the same day only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation results: Mean the corrected absorbance values (OD450-OD540 or OD570) and complex well readings for each standard and sample, and subtract the mean zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240816\/cdc6a213359045bda088ee0ddb22fdc9.jpg\" alt=\"\" width=\"384\" height=\"299\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels of human IL-10 were spiked into cell culture medium samples, and the recovery rate was determined. The recovery range is 88-110%, and the average recovery is 98%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human IL-10 is generally 3.9 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human IL-10 protein expressed by sf21.\u003cbr\u003e\u003cbr\u003e4. Linearity: 4 different samples were spiked with a high concentration of Human IL-10, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e98\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e92-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e96\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e86-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e94\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e84-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e90\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e82-98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003cbr\u003e\u0026lt; td style = \"width: 41.4371%; height: 22 px; \"\u0026gt; Improper storage of kits; mixed use of different kits\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2.73pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-10 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-10 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 10ELisa kit, CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL-10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-10 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman IL-10 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman IL-10 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 10 (IL-10), also known as cell growth factor synthesis inhibitor (CSIF), is a member of the IL-10α helix family, which also includes IL-19, IL-20, IL-22, IL-24, and IL-26\/ak155. IL-10 can be secreted by a variety of activated hematopoietic cells, hepatic stellate cells, keratinocytes, and placental trophoblast cells. Human IL-10 is active on mouse cells, while mouse IL-10 cannot act on human cells. Mature human IL-10 has 86% amino acid sequence homology with equine IL-10 and between 72-80% homology with bovine, canine, feline, guinea pig, mouse, sheep, pig, and rat IL-10. It contains two intra-chain disulfide bonds and is expressed as a non-covalently bound homodimer with a molecular weight of 36 kDa.\u003cbr\u003eIL-10 mediates its biological activity by a type II cytokine IL-10 receptor subunit alpha and beta heterodimer complex. The IL-10 receptor α subunit is a 110kDa transmembrane glycoprotein mainly expressed in lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, trophoblast cells and activated hepatic stellate cells, while the 75kDa transmembrane receptor β is widely expressed. The IL-10 dimer binds two IL-10Rα chains, triggering the addition of two IL-10Rβ chains. IL-10Rβ does not directly bind IL-10, but is required for signal transduction. IL-10Rβ also forms receptor complexes of IL-22, IL-26, IL-28, and IL-29 with IL-20Rα, IL-22Rα1, and IL-28Rα.\u003cbr\u003eThe immunomodulation involved in IL-10 includes inhibitory and stimulatory effects. It promotes M2 macrophages to exert anti-inflammatory effects by inhibiting the expansion and activation of Th1 cells and Th17 cells. The regulation of IL-10 expression by immunoregulatory T cells (Treg) and regulatory B cells is of great significance to Treg proliferation. However, in the tumor environment, IL-10 inhibits the proliferation of Treg and myeloid-derived suppressor cells. IL-10 induces accumulation and activation of CD8 T cells in tumors. IL-10 plays a protective role by limiting tissue damage in arthritis and promoting regeneration after muscle injury, but it may contribute to the persistence of viral infection. IL-10 levels are elevated in Sjogren's syndrome (saliva), primary central nervous system lymphoma (cerebrospinal fluid), ovarian cancer (serum and ascites). Its levels are reduced in the serum of patients with recurrent heart disease or pre-Zi Xian and in the semen of patients with male infertility.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518144979019,"sku":"abs510005-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4e9405679c2d4e7db249bb99ca7446eb.jpg?v=1792753461"},{"product_id":"human-tnf-elisa-kit","title":"Human TNF-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 1000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (1000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/f1687ac16b97408594e10501f750906e.jpg\" alt=\"\" width=\"466\" height=\"362\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of human TNF-α were spiked into cell culture medium samples, and the recovery rate was determined. The recovery range is 98-102%, and the average recovery is 100%.\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of human TNF-α is generally less than 0.68 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e3. Calibration: This ELISA kit was calibrated by high-precision recombinant human TNF-α protein expressed by E. coli.\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of human TNF-α, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 44.8366%; height: 100px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003eRecovery (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e1：1\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e87\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 极客时间; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e117\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 12px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 12px;\"\u003e104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant human TNF-α protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human TNF-α. Interference with human TNF-α was detected by incorporating 极客时间 ng\/mL of the interfering factor into the mid-range recombinant human TNF-α control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u0026lt; td style = \"width: 48.1332%; heightt: 22 px; \"\u0026gt; IL-1 β\u003ctable style=\"border-collapse: collapse; width: 47.5817%; height: 275px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-1ra\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-3\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-5极客时间;\u0026gt;\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-8\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-10\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-11\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 11px;\"\u003eTNF-β\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 11px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 极客时间;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u0026lt; tr style =\"height: 22px;\" \u0026gt;7Samples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tubeAvoid hemolysis, contamination, long storage and other phenomena\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.56pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAPC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 362px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman TNF-α Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman TNF-α Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman TNF-α detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE\/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa.\u003cbr\u003eTNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518145011787,"sku":"abs510006-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8de1367c5b3c4ec1996829ca550e10c1.jpg?v=1792753422"},{"product_id":"human-ifn-elisa-kit","title":"Human IFN-γ ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 600pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (600 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240329\/f4e5397eac094dc38495a48ca32f58c8.png\" alt=\"\" width=\"526\" height=\"302\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240329\/eb457e847cfd4cb4b8d847fb6eb26361.jpg\" alt=\"\" width=\"401\" height=\"312\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of human IFN-γ were spiked into cell culture medium samples, and the recovery rate was determined. The recoveries ranged from 90 to 116%, with an average recovery of 103%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of human IFN-γ is generally less than 7.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: This ELISA kit was calibrated by high-purity recombinant human IFN-γ protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of human IFN-γ, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.2527%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 33.4826%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 32.5948%; text-align: center; height: 22px;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 30.189%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 33.4826%; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 32.5948%; height: 22px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 30.189%; height: 22px;\"\u003e96-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 33.4826%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 32.5948%; height: 22px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 30.189%; height: 22px;\"\u003e107-115\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 33.4826%; height: 22px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 32.5948%; height: 22px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 30.189%; height: 22px;\"\u003e104-121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 33.4826%; height: 10px;\"\u003e1：16\u003c\/td\u003e\n\u003ctd style=\"width: 32.5948%; height: 10px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 30.189%; height: 10px;\"\u003e91-114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant human IFN-γ protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IFN-γ. Interference with human IFN-γ was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IFN-γ control. No significant cross-reactivity or interference was observed. There was approximately 20% cross-reactivity with recombinant rhesus IFN-γ.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u0026lt; td style = \"width: 24.0666%; text-align: center; height: 22 px; \"\u0026gt; Other recombinant proteins\u003ctable style=\"border-collapse: collapse; width: 59.1078%; height: 101px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 24.0666%; height: 22px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eIFN-β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eBovine IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eIFN-γR1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eMouse IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eCanine IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 13px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 13px;\"\u003ePorcine IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车开奖直播历史记录\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Sensitivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;0.519pg\/ml\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Species Reactivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Human\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IFN-γ antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IFN-γ present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Synonym\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Human interferon gamma ELISA kit, IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Please use it within the validity period of the kit (new and old products are shipped randomly)\u0026lt;\/strong\u0026gt;\u0026lt;br \/\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IFN-γ Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IFN-γ Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IFN-γ detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGamma interferon (also known as type II interferon) is an important cytokine that can exercise immunomodulatory function. It was discovered for its antiviral activity. Interferon gamma plays a key role in host defense through antiviral, antiproliferative, and immunomodulatory functions. In many types of cells, gamma interferon induces cytokine production and upregulates the expression of different membrane proteins, including type I\/II major histocompatibility complex (MHC-I, MHC-II), Fc receptors, leukocyte adhesion molecules, B7 family antigens. Gamma interferon can effectively activate macrophages and guide the synthesis, type transformation and secretion of B cell immunoglobulin. Gamma interferon can also affect the development of T helper cell subtypes by inhibiting the differentiation of Th2 and stimulating the growth of Th1. Gamma interferon plays an important role in some inflammatory disease processes such as autoimmunity and atherosclerosis.\u003cbr\u003eBiologically active gamma interferon is a non-covalently bound dimer with a molecular weight of about 20-25KD and varying degrees of glycosylation. Mature human interferon gamma has close to 90% homology with the amino acid sequence of interferon gamma from rhesus monkey, 59-64% homology with interferon gamma from cattle, dog, horse, cat and pig, and 37-43% homology with interferon gamma from cotton rat, mouse and rat. The interferon dimer first binds to the transmembrane receptor IFN-γRI (α subunit), and then binds to IFN-γRII (β subunit) to form a functional complex containing two α subunits and two β subunits; Wherein the IFN-γRII in the complex receptor can increase the affinity of the ligand as well as the signal transduction efficiency. Although the γ subunit is constitutively expressed in many types of cells, the β subunit cell expression regulates the influence of the response state of the receptor interferon γ and is strictly regulated.\u003cbr\u003eInterferon gamma can be secreted by many cells under a series of inflammatory conditions, including dendritic epidermal\/γδ T cells, keratinocytes, peripheral blood γδ T cells, mast cells, neurons, CD8 + T cells, macrophages, B cells, neutrophils, natural killer cells, CD4 + T cells and testicular sperm cells, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e9.38pg\/mL-600pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518145601611,"sku":"abs510007-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0c0578e7597f4fe0afb173e2c24b740f.jpg?v=1792753465"},{"product_id":"human-il-12-il-23-p40-elisa-kit","title":"Human IL-12\/IL-23 p40 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.84pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman IL-12 ELISA kit, IL-23 p19\/IL-12 p40, IL23, IL-23, IL-23A, IL-23-A, IL-23p19, IL-23p19\/IL-12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T-cell activation, MGC79388, SGRF, SGRFIL-23 subunit alpha,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eDetection principle:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003eDouble antibody sandwich ELISA was used in this experiment. Anti-human IL-12\/IL-23p40 monoclonal antibody is coated on a microplate, and IL-12\/IL-23p40 in samples and standards will bind to the antibody immobilized on the plate, and the free components will be washed away; Horseradish peroxidase-labeled anti-human IL-12\/IL-23 p40 polyantibody was added and unbound antibody was washed away; Adding a substrate solution (chromogenic agent), the color of the solution is proportional to the bound target protein; A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eTest Type:\u003c\/strong\u003eDouble antibody sandwich method\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eForm:\u003c\/strong\u003ePre-coated 96-well plate\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eTest Sample Type:\u003c\/strong\u003eCell supernatant, serum, plasma\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eSample Load:\u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003eA copy of the pre-coated 96-well plate, standard, anti-human IL-12\/IL-23 p40 detection antibody, buffer for dilution, chromogenic solution (A, B), wash solution, stop solution, SA-HRP, plate sealing membrane, and instructions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e3.5 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e62.5-4,000 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eRecovery Range:\u003c\/strong\u003e89-114%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eSave Method:\u003c\/strong\u003e2-8℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eStandard curve plot\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191225\/56811129d2cf4a2faad83cd56e78e8c2.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003eIL-12, also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine mainly produced by antigen-presenting cells (monocytes\/macrophages, dendritic cells, and B lymphocytes). IL-12 has a variety of effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production, and Th1 subset development.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003eIL-12 is a 70 kDa (p70) heterodimeric glycoprotein linked by disulfide bonds, consisting of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subbases do not possess IL-12 activity in themselves, but homodimers of p40 have been shown to bind IL-12 receptors and are IL-12 antagonists.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003eThe genes of human p40 and p35 located on chromosomes 5 and 3, respectively, are independently regulated. The expression of p35 mRNA has been found to be almost ubiquitous, however, the p35 subunit was not detected in the culture supernatant of cells expressing p35 or p35 and p40 mRNA only. The p40 mRNA expression level was higher in cells expressing both p35 and p40 mRNA, and together with the heterodimeric IL-12 p70, a free p40 subunit unrelated to the p35 subunit was secreted. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist in monomeric form. The content of free p40 far exceeds p70 in the supernatants of various activated human monocytes, and the levels of p70 measured by bioassays are consistent with those measured using p70-specific immunoassays, suggesting that p40 monomers are not potent IL-12 antagonists. Currently, the physiological role of the free p40 subunit is unclear.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518146617419,"sku":"abs510011-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8518fb8cbf4545cea494fc26d70428cd.jpg?v=1792753313"},{"product_id":"human-il-p70-elisa-kit","title":"Human IL-12 p70 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Color development solution: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The removed color development solution is only for the same day use.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240830\/0556058372d94ffaab92c059da9d99dc.jpg\" alt=\"\" width=\"355\" height=\"276\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate:\u003cbr\u003e\u003cbr\u003eDifferent levels of Human IL-12 p70 were spiked into cell culture medium samples and their recovery was determined. The recoveries ranged from 116 to 119%, with an average recovery of 117.6%.\u003cbr\u003e\u003cbr\u003eDifferent levels of Human IL-12 p70 were spiked into human serum samples and their recovery was determined. The recovery range is 97.9-105.9%, and the average recovery is 101.3%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human IL-12 p70 is generally 0.7 ng\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human IL-12 p70 protein expressed by Sf21.\u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were spiked with high concentrations of Human IL-12 p70, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"page-break-inside: avoid; border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e91.8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e83.8-98.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e94.6\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e81.9-104.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 极客时间; text-align: center;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e100.9\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e86.6-111.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e106.9\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e86.3-117.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant Human IL-12 p70 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-12 p70. Interference with Human IL-12 p70 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u0026lt; td style = \"text-align: center; width: 50%; \"\u0026gt; Recombinant human protein\u003ctable style=\"page-break-inside: avoid; border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"text-align: center; width: 50%;\"\u003eRecombinant mouse protein\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eIL-12 p35\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eIL-12 p70\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eIL-12\/IL-23 p40\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eIL-23\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"page-break-inside: avoid; border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 极客时间;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water极客时间\u0026gt;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"page-break-inside: avoid; border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.7ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-12 p70 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-12 p70 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 12p70 detection kit, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NKSF1, p35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"page-break-inside: avoid; border-collapse: collapse; width: 97.5262%; margin: 0px auto; break-inside: avoid; height: 184px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-12 p70 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman IL-12 p70 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman IL-12 p70 Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 52px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin 12 p70 (IL-12), also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine consisting of a 40kDa (p40) subunit and a 35kDa (p35) subunit. Human p40 may circulate as a monomer, a homodimer, or a complex that binds to p35 to form IL-12 or binds to p19 to form IL-23.\u003cbr\u003eBoth homodimers p40 and IL-23 can bind to IL-12R as non-signal antagonists. Alternatively, p40 can also bind to Rβ1, activating microglia and macrophages. The IL-12 p40 subunit shares with another heterodimeric cytokine, IL-23, with similar and distinct biological activity from IL-12. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8 + DCs (mouse cells only), and non-germinal center (CD38-CD44 +) B cells (human cells only). Functional IL-12 was shown to enhance cytotoxic activity and induce interferon gamma (IFN-gamma) production within NK cells, T cells, and dendritic epidermal T cells. IL12 has also been reported to induce IFN-γ production by macrophages. IL-12, together with other members of the IL-12 family, IL-23 and IL-27, promotes the development of CD4+Th1 immune response. In response to infection, IL-27 is first released, promoting the transition from Th0 to Th0\/1. IL-12 is subsequently produced, resulting in Th1 effector cells. IL-12 combines with IL-18 to generate Th1 memory cells from effector cells, which are subsequently activated by IL-23. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518146846795,"sku":"abs510012-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ed09378f92cc48409cae2468320abd05.jpg?v=1792753478"},{"product_id":"human-pd-l1-b7-h1-elisa-kit","title":"Human PD-L1\/B7-H1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e小于11.5pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274 antigenMGC142294, CD274 molecule, CD274, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003eDetection principle:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cspan style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human\u003cspan lang=\"EN-US\"\u003ePD-L1\/B7-H1\u003c\/span\u003eAntibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, the\u003cspan lang=\"EN-US\"\u003ePD-L1\/B7-H1\u003c\/span\u003eBinds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin was added\u003cspan lang=\"EN-US\"\u003e(Streptavidin-HRP)\u003c\/span\u003e。 After washing, a chromogenic substrate is added to protect the color from light. Add a stop solution to stop the reaction, and at\u003cspan lang=\"EN-US\"\u003e450 nm\u003c\/span\u003ewavelength\u003cspan lang=\"EN-US\"\u003e(\u003c\/span\u003eReference correction wavelength\u003cspan lang=\"EN-US\"\u003e540nm\u003c\/span\u003eOr\u003cspan lang=\"EN-US\"\u003e570nm)\u003c\/span\u003eThe absorbance value was determined.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003eDouble antibody sandwich method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003ePrecision\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003eIntra-plate coefficient of variation: 3.4%-5.8%; Coefficient of variation between plates: 1.3%-4.9%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003eSerum, plasma, cell culture supernatant and other biological samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eTest Sample Volume\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e5.58 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eDetection range\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e0.23 ng\/mL-15ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eRecovery\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e81%-104%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eAverage recovery\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e0.85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003ePlate type\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e96-well plate, detachable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eSave\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003eThe kit was stored unopened at 4 ℃. Disassembled, the standard is stored at-20 ℃, and the others are stored at 4 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eConditions of transportation\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e4 ℃ Blue Ice Shipping\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.7939%;\" width=\"271\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 84.6637%;\" width=\"1559\"\u003e96-well polystyrene plate coated with anti-B7-H1 monoclonal antibody\u003cbr\u003e\u003cbr\u003eHuman B7-H1 lyophilized standard\u003cbr\u003e\u003cbr\u003eB7-H1 detection antibody\u003cbr\u003e\u003cbr\u003eStandard dilution\u003cbr\u003e\u003cbr\u003eHorseradish peroxidase-labeled streptavidin\u003cbr\u003e\u003cbr\u003eDetection buffer (10x)\u003cbr\u003e\u003cbr\u003eSubstrate (TMB)\u003cbr\u003e\u003cbr\u003eStop liquid\u003cbr\u003e\u003cbr\u003eLotion (20 ×)\u003cbr\u003e\u003cbr\u003eSealing film\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003eStandard curve plot\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250217\/1a4137320df440668f8600eba486f4c3.jpg\" alt=\"\" width=\"181\" height=\"180\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003eBackground:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003eProgrammed death factor ligand\u003cspan lang=\"EN-US\"\u003e1 (PD-L1)\u003c\/span\u003e, also known as\u003cspan lang=\"EN-US\"\u003eB7-H1\u003c\/span\u003eAnd\u003cspan lang=\"EN-US\"\u003eCD274\u003c\/span\u003e, is an approximately\u003cspan lang=\"EN-US\"\u003e65 kDa\u003c\/span\u003eTransmembrane glycoproteins, belonging to\u003cspan lang=\"EN-US\"\u003eB7\u003c\/span\u003eFamily of immunomodulatory molecules.\u003cspan lang=\"EN-US\"\u003ePD-L1\u003c\/span\u003eExpressed in immune cells activated by inflammation, including macrophages,\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCells and\u003cspan lang=\"EN-US\"\u003eB\u003c\/span\u003eCells, keratinocytes, endothelial cells and intestinal epithelial cells, as well as various cancer cells and melanoma.\u003cspan lang=\"EN-US\"\u003ePD-L1\u003c\/span\u003eCan be with\u003cspan lang=\"EN-US\"\u003ePD-1\u003c\/span\u003eAnd\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCells\u003cspan lang=\"EN-US\"\u003eB7-1\/CD80\u003c\/span\u003eBinding, inhibition\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eActivation and proliferation of cells, inducing activated\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eApoptosis. It works by promoting\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCell inactivation and regulation\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCell development, which plays a role in the process of immune tolerance.\u003cspan lang=\"EN-US\"\u003ePD-L1\u003c\/span\u003eBeneficial to the anti-inflammatory process\u003cspan lang=\"EN-US\"\u003eIL-10\u003c\/span\u003eAnd\u003cspan lang=\"EN-US\"\u003eIL-22\u003c\/span\u003eDevelopment of dendritic cells, inhibition of\u003cspan lang=\"EN-US\"\u003eTh17\u003c\/span\u003eDevelopment of cells. In cancer,\u003cspan lang=\"EN-US\"\u003ePD-L1\u003c\/span\u003eAble to resist\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCell-mediated lysis, enhanced the ability of epithelial cells to transition to mesenchyma, enhanced\u003cspan lang=\"EN-US\"\u003eTh22\u003c\/span\u003eThe tumorigenic function of cells. In plasma of cancer patients and cerebrospinal fluid of glioma patients, its soluble\u003cspan lang=\"EN-US\"\u003ePD-L1\u003c\/span\u003eIt will rise somewhat. It can be released by mature dendritic cells and retains binding\u003cspan lang=\"EN-US\"\u003ePD-1\u003c\/span\u003eAnd induction\u003cspan lang=\"EN-US\"\u003eT\u003c\/span\u003eCapacity of apoptosis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518146879563,"sku":"abs510014-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bd8f68400c4e442096933344f9fa3ec6.jpg?v=1792753485"},{"product_id":"human-il-17-il-17a-elisa-kit","title":"Human IL-17\/IL-17A ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.15pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human IL-17 monoclonal antibody is coated on the microplate. IL-17 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-17 multi antibody was added to combine with IL-17 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human IL-17 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6 - 1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cstrong\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/796a698f8b2e40808ea842c1cff91208.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe IL-17 family is composed of at least six pro-inflammatory cytokines, which share a conserved cysteine knot structure, but differentiate at the N terminus. IL-17 family members are glycoproteins secreted as dimers, which can induce the production of local cytokines and recruit granulocytes to the site of inflammation. IL-17 is composed of IL-17 and il-23-induced, mainly in activated cd4+ T cells, unlike Th1 or Th2 cells. IL-17F has the strongest homology with IL-17, but is only induced by IL-23 in activated monocytes. IL-17B binds to the IL-17B receptor, but not to the IL-17 receptor, and has the strongest homology with il-17d, which is expressed by resting cd4+ T cells and cd19+ B cells. Il-17e is mainly produced by Th2 cells and recruits eosinophils to lung tissue. The expression pattern of il-17c is very limited, but it has been detected in adult prostate and fetal kidney banks.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518148091979,"sku":"abs510019-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a1f5c2904ce841309111fbf4bd8fc2ca.jpg?v=1792753322"},{"product_id":"human-ccl2-mcp-1-elisa-kit","title":"Human CCL2\/MCP-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30.5pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti human ccl2\/mcp-1 capture antibody is coated on the microplate. After incubation, ccl2\/mcp-1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2\/mcp-1 detection antibody was added for incubation. After washing to remove unbound substances, streptavidin HRP was added for incubation. After washing, the chromogenic substrate was added to avoid light for color development. The solution color is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plates, standards, anti human   One copy of ccl2\/mcp-1 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e18.9  Pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6 - 1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85-104%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191227\/b50a847d1c4a4e2e8ad0070965c653d4.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCCL2\/MCP-1 is a chemokine that binds to the receptor CCR2 and induces chemoattraction of monocytes. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to inflammatory sites exacerbates atherosclerosisSeverity of sclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518149107787,"sku":"abs510026-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e927172b035c4bc8a8f55f87b14e1266.jpg?v=1792753344"},{"product_id":"human-cxcl10-ip-10-elisa-kit","title":"Human CXCL10\/IP-10 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e6. Microplate oscillator \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eI. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e(1) cell culture supernatant: particulate matter should by centrifugal removing; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 2000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. No dilution of standard mother liquor can be used as a standard curve peak (2000 pg\/mL), dilute with buffer (1 \u0026amp; times) Can be used as a standard curve zero (0 pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/3ca1b770c3c2458896bdd173379097de.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Ready for all the needed reagent and standard; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231221\/35143f092c344b43aaadd43e53411247.jpg\" alt=\"\" width=\"339\" height=\"264\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery: Different levels of Human IP-10 were incorporated into the cell culture medium samples, and the recovery rate was determined. Recoveries ranged from 78 to 114%, with an average recovery of 93%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of Human IP-10 is generally less than 3.5pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 10 standard curves plus two standard deviations. \u003cbr\u003e3. Calibration: The standard of this ELISA kit is a high-purity recombinant HumanIP-10 calibrated by Epixin. \u003cbr\u003e4. Linearity: Five different samples were mixed with high concentrations of Human IP-10, followed by dilution (1×) Linearity was determined by dilutes the samples to the detection range.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-116\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e98\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e86-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e95\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e89-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e101\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e89-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e5. Specificity: The ELISA can detect native and recombinant Human IP-10 protein. The following factors in diluent mixture 50 ng\/mL (1 x) to detect the concentration and the Human IP - 10 cross reaction. To the interference of 50 ng\/mL factor added to the middle range of recombinant Human IP - 10 reference substance, to detect interference with Human IP - 10. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.3268%; height: 296px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px; width: 48.1332%;\" colspan=\"2\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant rat proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eBLC\/BCA-1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eBLC\/BCA-1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eENA-78\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-18\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGCP-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGCP-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eNAP-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGROα\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eNGR1-β1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIFN-γ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGROβ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eMCP-1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eGROγ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eLymphotactin\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eTNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eI-TAC\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eMIG\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIFN-γ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eSDF-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-10\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eSDF-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-17A\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eTGF-β1\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIP-10\/CRG-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eKC\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-5\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eTYK-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eSDF-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eVEGF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height极= 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 极22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.7pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-human CXCL10\/IP-10 antibody was precoated on a high-affinity microplate. After incubation, CXCL10\/IP-10 in the sample was combined with the solid-phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman CXCL10\/IP-10 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman CXCL10\/IP-10 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman CXCL10\/IP-10 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChemokine ligand 10 (CXCL10), also known as interferon-inducible protein 10 (IP-10) or micro-inducible cytokine B10, is a small molecular weight cytokine belonging to the CXC chemokine family. Many types of cells can secrete IP-10 in response to IFN-γ, including monocytes, endothelial cells and fibroblasts. IP - 10 has a variety of functions, such as the monocyte\/macrophage, T cells, NK cells and dendritic cell chemotaxis, promote T cell adhesion to endothelial cells, antitumor activity and inhibition of bone marrow colony forming and angiogenesis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518150123595,"sku":"abs510027-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/afbdf97061fb4f9faf5c376ad2e43b19.jpg?v=1792753348"},{"product_id":"human-ccl3-mip-1-alpha-elisa-kit","title":"Human CCL3\/MIP-1α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.99pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human mip-1α The monoclonal antibody was coated on the microplate, and mip-1\u0026amp;alpha in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human mip-1\u0026amp;alpha was added; Polyclonal antibody, and mip-1\u0026amp;alpha bound on the microplate; Combine to form immune complexes, and the free components are washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plates, standards, anti human mip-1α One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e10 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81 - 500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85.0-111.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20230111\/ee20280ca98c469c8a17a9634b278cb0.png\" width=\"300\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMip-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily, which was initially purified from the conditioned medium of LPS stimulated murine macrophage cell lines. Mip-1\u0026amp; alpha; is highly effective on a variety of cells (including monocytes, T cells, B cells, and eosinophils)Plays a chemotactic role.\u003cbr\u003eTwo human mip-1α Genes are generated by duplication \/ mutation. They encode mip-1α Isoforms CCL3 \/ ld78a and CCL3L1 \/ ld78b, which share 94% amino acid sequence homology. Human CCL3 \/ ld78a is a single copy gene, while human CCL3L1 \/ ld78b gene copy number varies among populations. Human CCL3L1 \/ ld78b binds and signals through chemokine receptors CCR1, CCR5. Compared with CCL3 \/ ld78a, CCL3L1 \/ ld78b has higher binding affinity for CCR5, which is also a co receptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518150385739,"sku":"abs510029-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7cfd5a4857f249e7bf46c4660ee9de73.jpg?v=1792753363"},{"product_id":"human-osteoprotegerin-tnfrsf11b-elisa-kit","title":"Human Osteoprotegerin\/TNFRSF11B ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human OPG monoclonal antibody is coated on the microplate. OPG in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti human OPG multi antibody was added to combine with OPG bound on the microplate, and the free components were washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection sample type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human OPG detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e6.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e62.5 - 4000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85.0-108.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃ \u003c\/p\u003e\n\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003cp\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20200814\/0ed2a7cc1d0a485b8fe5cb018447a61c.jpg\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOsteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone mineral density. OPG is widely expressed and released by mesenchymal stem cells, fibroblasts, and endothelial cells in the form of homodimers. OPG has the decoy receptor function of trace \/ Rank l and trail. OPG is involved in regulating the expression of trace on the cell surface and its ability to stimulate osteoclast formation. Dysregulation of OPG \/ trance interaction leads to Paget's disease, osteoporosis, bone loss, and vascular calcification.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518150418507,"sku":"abs510031-96T","price":450.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-il-1-elisa-kit","title":"Mouse IL-1β ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e\n1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\n2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\n3. Distilled water or deionized water\u003cbr\u003e\n4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\n5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\n\n\u003cstrong\u003eOne, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\n1. Sample collection and storage\u003cbr\u003e\n① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e)\u003cbr\u003e\n1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\n②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\n③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\n④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\n⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\n⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\n\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/9297927b5c1b4d6dbdde6fd6fcaad53d.png\" alt=\"\" width=\"433\" height=\"245\"\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n1. Ready for all the needed reagent and standard;\u003cbr\u003e\n2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\n3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\n4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\n5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\n6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\n7. Repeat the plate washing operation of step 5;\u003cbr\u003e\n8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\n9. Repeat step 5 to wash the plate;\u003cbr\u003e\n10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\n11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\n12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\n13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/6748f65fcdda465ba54e1c682648f699.jpg\" alt=\"\" width=\"312\" height=\"242\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n1. Recovery: Different levels of Mouse IL-1\u0026amp;beta were incorporated into the cell culture medium samples; And measure its recovery. The recovery ranges from 69 to 102%, with an average recovery of 77%.\u003cbr\u003e\n2. Sensitivity: Mouse IL-1β The minimum detectable dose (MDD) is generally less than 3.8 pg\/mL. The lowest detectable dose was calculated as the corresponding concentration based on the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\n3. Calibration: The ELISA kit was purified recombinant Mouse IL-1\u0026amp;beta expressed in E. coli. Corrected by protein.\u003cbr\u003e\n4. Linearity: four different samples were mixed with high concentrations of Mouse IL-1β , and then with diluent (1×) Measure linearity by dilating the sample to within the detection range.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e101-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e105-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n5. Specificity: The ELISA could detect native and recombinant Mouse IL-1\u0026amp;beta. Protein. Combine the following factors with diluent (1×) Configure to a concentration of 50ng\/mL to detect with Mouse IL-1β Cross reaction with mouse IL-1 \u0026amp; beta. 50ng\/mL of interfering factor was incorporated into the intermediate range of recombinant Mouse IL-1β To detect the effect on Mouse IL-1β The interference. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1Ra\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RI\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003eIL-1RII\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height极客 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too many, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 极客 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-1β antibody was precoated on a high-affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-1β present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-1β Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-1β standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-1β detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe interleukin-1 (IL-1) protein family contains the classical members IL-1α, Il-1β and IL-1ra, as well as IL-18, IL-33 and IL-1F5-10. IL-1α and IL-1β bind to the same cell surface receptor and share biological functions. \"With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, unstimulated cells from healthy mice do not produce IL-1.\" \"However, in response to inflammatory agents, infections, or microbial endotoxins, IL-1 is produced in large quantities by macrophages and a variety of other cells.\" IL-1β plays a key role in the physiological processes of immune response and inflammation, bone remodeling, fever, glucose metabolism, growth hormone \/IGF-1. \"Abnormal or sustained production of IL-1 has been associated with a range of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and aging-related diseases.\" \u003cbr\u003eIL-1α and IL-1β have structurally related peptides that share approximately 25% identity at the amino acid level. They were synthesized as the size of 31 kDa precursor, and then cracking into mature protein, molecular weight is about 17.5 kDa. Caspase 1 \/ ICEI cracking IL - 1 beta precursor is a key step in the inflammatory response. Neither IL-1α nor IL-1β contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through nonclassical pathways. Part of raw IL - 1 alpha can appear on the cell membrane, and may have biological activities. Unlike precursor IL - 1 alpha, beta IL - 1 precursor compared with mature body almost do not have biological activities. Both unprocessed and mature IL-1β can be exported from the cell. \u003cbr\u003eIL - 1 alpha and beta IL - 1 with IL - 1 ra combination immunoglobulin superfamily receptors play a role. \"Cells expressing the 80-kda transmembrane type I receptor (IL-1RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid lining cells, chondrocytes, and hepatocytes.\" \"Cells expressing the 68kDa transmembrane type II receptor (IL-1RII) include B cells, neutrophils, and bone marrow cells.\" \"The two IL-1 receptors share approximately 28% identity in their extracellular domains, but a significant difference is found in their intracellular domains: the type II receptor has an intracellular domain of only 29 amino acids, whereas the type I receptor has an intracellular domain of 217 amino acids.\" IL-1RII does not produce a signal for IL-1 and may act as a decoy receptor to attenuate IL-1 function. IL-1 receptor accessory protein (IL-1RAcP) is associated with IL-1RI and required for IL-1RI signal transduction. IL-1ra is a secretory molecule that acts as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII can be detected in human plasma, joint synovial fluid, and conditioned media of a variety of human cell lines. In addition, IL-1binding proteins similar to soluble IL-1RII are capable of being encoded by cowpox and vaccinia virus.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518150975563,"sku":"abs520001-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7ac31d984f114771b7dbeb565f671ef6.jpg?v=1792753492"},{"product_id":"mouse-il-elisa-kit","title":"Mouse IL-10 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e18.5pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse il-10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-10present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse il-10detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.2 - 2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e95-103%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMouse interleukin -10 (IL-10) also known as cytokine synthesis inhibitor (CSIF). It is IL-10 \u0026amp; Alpha-a member of the helical cytokine family, which also includes il-19, il-12, il-22, IL-24 , and il-26\/ak155. Many activated hematopoietic stem cells, hepatic stellate cells, keratinocytes, and placental cytotrophoblasts secrete il-10. Human IL-10 is active in mouse cells, but mouse IL-10 does not act on human cells. The amino acid homology of mature mouse IL-10 and rat IL-10 is 85%, and that of cattle, dogs, horses, cats, humans, sheep, and pigs is 70~77%. Mature mouse IL-10 is a noncovalently bound homodimer with a molecular weight of 36 kDa containing two intrachain disulfide bonds.\u003c\/p\u003e\n\u003cp\u003eIL-10 by the II type cytokine receptor IL-10 r\u0026amp; Alphaand IL-10 \u0026amp; amp; Betathe heteromeric receptor complex that is composed of exerts its biological activityIL-10 r\u0026amp; Alphais a 110 kDa transmembrane glycoprotein expressed in lymphocytes, natural killer cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while 75 kDa il-10r\u0026amp; Beta\u003c\/p\u003e\n\u003cp\u003eIL-10 is involved in immune regulation of inhibitory and activating effectsIL-10 as an anti-inflammatory cytokine, promotes the formation of M2 macrophages by inhibiting the activation and expansion of Th1 cells and Th17 cells. Immunosuppression regulates the expression of T cells and B cells, which play an important role in Treg cell proliferation (19) . In the tumor microenvironment, IL-10 inhibition regulates the proliferation of T cells and myeloid derived suppressor cellsIL-10 induces the accumulation of intratumoral material and activates cd8+ T cellsIL-10 plays an important protective role in limiting tissue damage in joint inflammation and promoting muscle regrowth after injury, but also contributes to persistent viral infection. IL-10 is elevated in Sjogren's syndrome (saliva) and primary central nervous system lymphoma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152220747,"sku":"abs520005-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/773aa4393f904fd0b8ef1159a663e229.jpg?v=1792753513"},{"product_id":"mouse-il-p70-elisa-kit","title":"Mouse IL-12 p70 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 2500pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (2500pg\/mL), dilute with buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/9fc40f0430674440a0dca6993aaabcf5.png\" alt=\"\" width=\"555\" height=\"286\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/31104240a4094db3bbb14da2f3b0bdec.jpg\" alt=\"\" width=\"249\" height=\"194\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided by \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery: Different levels of mouse IL-12 p70 were incorporated into the cell culture medium samples, and the recovery rate was determined. Recoveries ranged from 83 to 107%, with an average recovery of 93%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of murine IL-12 p70 was generally less than 2.24pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 12 p70 protein in mice. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse IL-12 p70, followed by dilution (1×). Linearity was determined by dilating the samples to within the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 80.7041%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e99-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e104\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-112\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e93-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e5. Specificity: the ELISA method to detect natural and restructuring IL - 12 p70 protein in mice. The following factors in diluent mixture 50 ng\/mL (1 x) to detect the concentration of IL - 12 p70 cross reaction with mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 12 p70 reference substance in the restructuring of the mice, to detect the interference of IL - 12 p70 in mice. No significant cross-reactivity or interference was observed. The recombinant rat IL-12 p70 containing 1.25ng\/mL had cross reactivity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 132px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p70\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p35\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p40 monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p40 monomer\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL - 12 p40 homologous dimers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit with double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti mouse antibody of IL - 12 p70 pre package is on the high affinity enzyme label plate. After incubation, the IL-12 p70 in the sample was combined with the solid phase antibody and the detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-12 p70 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-12 p70 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-12 p70 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-12 (IL-12, also known as NKSF), a 70-75 kda heterodimer glycoprotein, belongs to the IL-12 heterodimer cytokine family. IL - 12 by 35 kda (p35) and 40 kda (p40) of two subunits, the two subunits by disulfide bond is linked together, no amino acid sequence homology between each other. The mature p35 subunit is 196 amino acids long, containing seven cysteines and a potential N-linked glycosylation site. Mature mouse p35 shares 63% and 86% amino acid identity with human and rat, respectively. Mature mice p40 subunits long 313 amino acids, including 13 cysteine and five potential glycosylation sites of N - connection. Some studies have reported that the p40 sequence is polymorphic, but this IL-12 p70 kit does not recognize these allelic products. \u003cbr\u003emature mouse p40 has 72% and 93% amino acid identity with human and rat, respectively. Although p35 resembles a ligand for erythropoietin, p40 is more similar to the N-terminus of the erythropoietin receptor, with WSXWS domains, an immunoglobulin-like domain, and four conserved cysteines. This suggests that IL-12 may be a cytokine receptor mimetic, similar to the IL-6\/ soluble IL-6R complex. It is worth noting that the p40 can exist in the form of monomer or homologous dimers, but never found p35 monomer form. p40 is the larger of the two subunits of interleukin 23 (IL-23). Although IL-12 has long been considered a secretory molecule, membrane-bound IL-12 has also been found in human and mouse cells. Il-12-producing cells include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8+DC (mouse cells only), and non-germinal center (CD38-CD44+) B cells (human cells only). The high-affinity receptor for \u003cbr\u003emouse IL-12 is composed of at least two type I transmembrane glycoproteins, similar to members of the cytokine receptor superfamily. Beta 1 first subunits (R) of 100 kda, with Kd = 1 nm and IL - 12. This receptor is the primary binding site for the p40 subunit; The second subunit (Rβ2) is 130kDa and has no amino acid homology with the Rβ1 subunit. The receptor is an important part of signal transduction, as a disulfide bond connected p30 - p40 dimers attachment points. As mentioned above, mouse p40 exists as a monomeric, dimeric form, and binds either at p35 to form IL-12 or at p19 to form IL-23. Both homodimer p40 and IL-23 can bind IL-12R and act as antagonists that do not transmit signals. Alternatively, p40 homodimer can also bind to Rβ1 and activate microglia and macrophages. Functionally, IL-12 has been shown to enhance cytotoxicity and induce interferon-γ production by NK cells, T cells, and dendritic epidermal T cells. It has been reported that IL-12 can induce the production of interferon-γ by macrophages. Together with its IL-12 family members such as IL-23 and IL-27, IL-12 can promote the progression of CD4+ Th1-type immune responses. In response to infection, IL-27 is secreted first to promote the excess of TH0 to THR0\/1, followed by IL-12 secretion to generate Th1 effector cells. \"Production together with IL-18 and IL-12 transforms effector cells into Th1 memory cells, which are then activated by IL-23.\"\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eUnopened kits were stored at 2-8 ° C. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e39.06pg\/mL-2500pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152253515,"sku":"abs520006-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/194789392b3e421988c988138a86cbf3.jpg?v=1792753510"},{"product_id":"mouse-ifn-elisa-kit","title":"Mouse IFN-γ ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 2000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (2000pg\/mL), and the dilution buffer (1×) Can be used as a standard curve zero (0 pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/3ca1b770c3c2458896bdd173379097de.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution. \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/ba161fb276f04f058cee473d92d99563.jpg\" alt=\"\" width=\"310\" height=\"241\"\u003e\u003cstrong\u003e\u003cstrong\u003eNote: The standard curve data provided is for reference only. The sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery: Different levels of mouse IFN-\u0026amp;gamma were incorporated into the cell culture medium samples; And the recovery rate was determined. The recovery ranges from 82 to 110%, with an average recovery of 94%. \u003cbr\u003e\u003cbr\u003e2. Sensitivity: mouse IFN-γ The minimum detectable dose (MDD) of murine IFN - \u0026amp; gamma was generally less than 1.8pg\/mL. The lowest detectable value is the corresponding concentration calculated from the mean of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was prepared with highly purified recombinant mouse IFN-\u0026amp;gamma expressed in E. coli. Corrected by protein. \u003cbr\u003e\u003cbr\u003e4. Linearity: four different samples were mixed with high concentrations of mouse IFN-γ , and then with diluent (1×) Measure linearity by dilating the sample to within the detection range. \u003cbr\u003e\u003cbr\u003e5. Specificity: The ELISA could detect both native and recombinant mouse IFN-γ Protein. Combine the following factors with diluent (1×) Formulated to a concentration of 50ng\/mL to detect the difference with mouse IFN-γ The cross reaction. 50ng\/mL interfering factor was incorporated into the intermediate range of recombinant mouse IFN-γ To detect the effect of IFN-\u0026amp;gamma on mice; The interference of IFN - \u0026amp; gamma. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e4, common problem resolution\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit with double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse IFN-γ antibody was precoated on a high-affinity microplate. Enzyme label plate hole with standard substance, the sample under test and biotinylated antibodies detection, after incubation, samples of IFN - gamma combined with solid phase antibody and detection antibody, immune complex. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IFN-γ Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IFN-γ standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IFN-γ detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterferon-γ (IFN-γ, also known as type II interferon) is an important immunomodulatory cytokine, which has been found to have antiviral activity. IFN-γ plays a key role in the host defense process through its antiviral, antiproliferative and immunomodulatory functions. \"In many cell types, IFN-γ induces cytokine production and upregulates the expression of a variety of membrane proteins, including types I and II major histocompatibility complex (MHC) antigens, Fc receptors, leukocyte adhesion molecules, and B7 family antigens.\" IFN-γ is a potent activator of macrophage response. It directs the synthesis, conversion, and secretion of immunoglobulin by B cells. IFN-γ affects the development of T helper cell phenotype by inhibiting the differentiation of Th2 cells and stimulating the development of Th1 cells. IFN-γ plays an important role in the progression of autoimmune diseases and inflammatory diseases such as atherosclerosis. \u003cbr\u003ebioactive IFN - gamma is made up of two non covalently linked with different degree of glycosylation of 20 to 25 kda homologous dimer composed of subunits. Mature mouse IFN-γ shared 86% amino acid sequence identity with rat IFN-γ, and 38-44% amino acid sequence identity with bovine, dog, cotton rat, horse, cat, human, pig and rhesus monkey IFN-γ. The IFN-γ dimers assemble before the transmembrane IFN-γRI(α subunit) and then bind to the transmembrane IFN-γRII(β subunit), thus forming an active receptor complex containing two α and β subunits. IFN-γRII in the receptor complex increases ligand affinity as well as the efficiency of signal transduction. Although the α chain is widely expressed in a variety of cell types, the receptor β chain is associated with the state of IFN-γ response and its expression is tightly regulated. \u003cbr\u003eUnder inflammatory conditions, IFN-γ can be produced by a variety of cell types, including dendritic epidermal \/γδT cells, keratinocytes, peripheral γδT cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, natural killer cells, CD4+T cells, and testicular spermatids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152351819,"sku":"abs520007-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6efb12a8026c42c0a9719bf933732ba2.jpg?v=1792753515"},{"product_id":"mouse-tnf-elisa-kit","title":"Mouse TNF-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1.  Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2.  High precision liquid dispenser and disposable tip \u003cbr\u003e3.  Distilled or deionized water \u003cbr\u003e4.  Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e1.  Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e2.  Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand  \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 2000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 2000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/3ca1b770c3c2458896bdd173379097de.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e1.  Prepare all required reagents and standards; \u003cbr\u003e2.  Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3.  Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4.  Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5.  The liquid in the plate is sucked off and the plate is washed using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the intra-plate wash liquid is aspirated. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6.  Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7.  Repeat th 5 Step washing operation; \u003cbr\u003e8.  Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9.  Repeat th 5 Step washing operation; \u003cbr\u003e10.  Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11.  Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12.  After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13.  Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/c1c8507ef90b434c97bb474ed5498e92.jpg\" alt=\"\" width=\"381\" height=\"296\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e1.  Recovery: Different levels of mice were spiked in cell culture medium samples TNF-α The recovery rate was determined. The recoveries range from 88-107% , the average recovery was in 94% 。 \u003cbr\u003e2.  Sensitivity: Mouse TNF-α The lowest measurable dose ( MDD ) is generally less than 5.5pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e3.  Correction: This ELISA High purity recombinant mice expressed by Escherichia coli TNF-α Corrected by protein. \u003cbr\u003e4.  Linearity: 4 High concentrations of mice were spiked into different samples TNF-α , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003e Dilution factor \u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003e Average \/ Expected value ( % ） \u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003e Range ( % ） \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e97\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e94-103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e99-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e97-114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e104-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5.  Specificity: This ELISA Method can detect natural and recombinant mice TNF-α Egg whites. The following factors were mixed with diluent ( 1× ) formulated into  50ng\/mL Concentration to detect with mice TNF-α Cross-reactivity of. Will 50ng\/mL Interfering factors incorporated into the intermediate range of recombinant mice TNF-α In the control article, to detect the effect on mice TNF-α Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.3268%; height: 120px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003e Recombinant human protein \u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003e Recombinant mouse protein \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eLymphotoxin α1\/β2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF RII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eLymphotoxin α2\/β1\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF RI\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eTNF RI\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e1.  Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Improper storage of kits; Mixing reagents of different kits \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Purchase new kits and pay attention to storage conditions; Not mixable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Low incubation temperature and short time \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e If the temperature is low, extend the incubation time and color development time \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Wrong addition or missed addition of reagents \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Add the correct reagent strictly according to the instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e The container used to prepare the solution is not clean or there is a problem with the water \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Use clean containers and qualified distilled water \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e In the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Follow the instructions strictly \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Heterogeneity of reagent temperature \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e All reagents should be equilibrated at room temperature 30 minute \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003e Detecting antibodies and \/ Or HRP Concentration too low \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003e Refer to the instructions, do not dilute at will \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2.  Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Less washing times, insufficient \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Wash as per instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Substrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Use clean containers and qualified distilled water during preparation; Store protected from light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Incubation temperature is high and \/ Or too long \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Controlling the temperature and time of incubation and final enzymatic reaction \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e The gun tip was not changed during sample loading, resulting in cross-contamination \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Change the gun head for each sample \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Cross contamination of nearby holes \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Vertical clapper, use suitable clapper paper to avoid paper scraps in the hole \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Presence of endogenous interfering substances in sample \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Estimate possible infectious substances and perform corresponding treatment \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003e Samples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003e Avoid hemolysis, contamination, long storage and other phenomena \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 5. Experimental flow chart \u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.0pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse TNF-α antibodies were pre-coated on high affinity enzyme labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAPC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNFA, TNF-A, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha, Mouse tumor necrosis factor alpha ELisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003e form \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003e Specifications \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003e Shelf life of diluted or redissolved reagent after unpacking \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse TNF-α Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 Block \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003e After the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse TNF-α Standard \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003e After dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse TNF-α Detection antibodies \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e After the concentrated volume is dissolved, it can be in 2-8°C store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10× Buffer for concentration and dilution \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003e After opening, it can be found in 2-8°C store 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003e Chromogenic liquid \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003e Stop Solution \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20× Concentrated Wash Buffer \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e Sealing film \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 Sheets \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003e Stored at room temperature, to avoid contamination, not reusable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototype ligand of tumor necrosis factor superfamily. It is a pleiotropic factor that plays a central role in inflammatory response, immune system development, apoptosis and lipid metabolism. TNF-α is also involved in many pathological processes, including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type II diabetes, septic shock, autoimmunity, and cancer. Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE\/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa.\u003cbr\u003eTNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152974411,"sku":"abs520010-96T","price":317.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2daa7c3a973247cab17a0308debd0bd8.jpg?v=1792753523"},{"product_id":"il-13-elisa-kit","title":"Mouse IL-13 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"434\" height=\"244\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Ready for all the needed reagent and standard; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240313\/424653e603b94cd38f1c549a9c8f408c.jpg\" alt=\"\" width=\"278\" height=\"216\"\u003eNote: The standard curve data provided is for reference only. The sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery: Different levels of mouse IL-13 were incorporated into the cell culture medium samples, and the recovery rate was determined. The recovery ranged from 96 to 103%, with an average recovery of 99%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-13 in mice was generally less than 0.089-0.740pg\/mL. The lowest detectable value of IL-13 was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 13 proteins in mice. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse IL-13, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e100-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e102-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e103-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e100-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse IL-13 protein. The following factors in diluent (1 x) configured to the concentration of 50 ng\/mL to detect cross reaction with IL - 13 in mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 13 reference substance in the restructuring of the mice, the interference of IL - 13 to test in mice. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 108px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\" colspan=\"2\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 20px; text-align: center;\" colspan=\"2\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eG-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIL-10R\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eLIF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIFN-γ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eMIP-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eMIP-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.65pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-13 antibody was precoated on a high-affinity microplate. After incubation, the IL-13 present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-13 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-13 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-13 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-13 (IL-13), a member of the chemokine family, plays a key role in the pathogenesis of allergy, cancer and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils and visceral smooth muscle cells. IL-13 inhibits the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE and upregulation of CD23\/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha1 and IL-4 R alpha. The compound is used as a type 2 receptor IL - 4. IL-13 ralpha2 binds IL-13 and prevents IL-13 from signaling through the IL-13R alpha1\/IL-4 Ralpha complex. Il-13-bound IL-13 R alpha2 can directly promote tumor cell invasion and tissue fibrosis development.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153203787,"sku":"abs520012-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/71872e1ce512478d800d1f2b4fde2c06.jpg?v=1792756817"},{"product_id":"mouse-cxcl10-elisa-kit","title":"Mouse CXCL10\/IP-10\/CRG-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\u003cbr\u003e5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards\u003c\/strong\u003e\u003cstrong\u003edo double hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample:\u003c\/strong\u003e Take 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample:\u003c\/strong\u003e Take 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample:\u003c\/strong\u003e 10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample:\u003c\/strong\u003e used 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"434\" height=\"244\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/b1974362223e44e183d920c17ee108df.jpg\" alt=\"\" width=\"302\" height=\"235\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in\u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery: Different levels of mouse CXCL10\/IP-10\/CRG-2 were incorporated into the cell culture medium samples to determine the recovery rate. The recovery ranged from 90 to 116%, with an average recovery of 103%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of murine CXCL10\/IP-10\/CRG-2 was generally less than 7.8pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with recombinant mouse CXCL10\/IP-10\/CRG-2 protein expressed in E. coli.\u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse CXCL10\/IP-10\/CRG-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e96-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e107-115\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e104-121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e91-114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse CXCL10\/IP-10\/CRG-2 proteins. The following factors were diluted (1×) A concentration of 50ng\/mL was formulated to detect cross-reactivity with mouse CXCL10\/IP-10\/CRG-2. To the interference of 50 ng\/mL factor added to the middle range of restructuring the mice CXCL10 \/ IP - 10 \/ CRG - 2 reference substance, to test in mice CXCL10 \/ IP - 10 \/ CRG - 2. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 108px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 20px; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 20px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eI-TAC\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIP-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eLIX\/GCP-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eMIG\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003ePF-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车开奖直播历史记录\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse CXCL10\/IP-10\/CRG-2 antibodies were precoated on high-affinity microplate. After incubation, CXCL10\/IP-10\/CRG-2 present in the sample combined with the solid-phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChemokine (C - X - C motif) ligand 10 (CXCL10) belongs to CXC chemokines family a small cytokines, also known as 10 - kda interferon gamma induced proteins (beta IP10 or IP - 10). In response to IFN-α, CXCL10 is secreted by several cell types. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 is thought to have a variety of effects, such as chemoattractant to monocytes and T cells, promotion of T cell adhesion to endothelial cells, anti-tumor activity and inhibitory effects on bone marrow colony formation and angiogenesis. IP-10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. CRG-2, the mouse homolog of human IP-10, shares approximately 67% amino acid sequence identity with human IP-10.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153236555,"sku":"abs520013-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8ce06aa8ec8147989f36bf994ebc6a2d.jpg?v=1792756819"},{"product_id":"ccl5-elisa-kit","title":"Mouse CCL5\/RANTES ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.2pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse ccl5\/rantes antibody was pre coated on a high affinity microplate. Standards, samples to be tested and biotinylated detection antibodies are added into the wells of enzyme plate. After incubation, mouse ccl5\/rantes present in the samples are combined with solid-phase antibodies and detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse ccl5\/rantesdetection antibody, dilution buffer, chromogenic solution (a, b), washing liquid, stop liquidSa-hrp, sealing film and instruction manual.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.3-2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003cstrong\u003e96-103%\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520014.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520014.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eChemotactic cytokineChemotaxis to leukocytes is an important initial step in the process of inflammation, and it is also an important aspect of the body's innate immune function in defense and clearance of foreign bodies such as invading pathogens. RANTES, which regulates the expression and secretion of normal T cells, is a member of the CC subfamily of chemotactic cytokines, also known as CCL5. The protein has a wide range of sources, most of which are expressed in the inflammatory factor IL-1 \u0026amp; amp; Beta; Or tnf-\u0026amp; Alpha; CCL5 can be released under the stimulation of. Generally, CCL5 is mainly secreted by cd8+t cells, epithelial cells, fibroblasts and platelets.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eCCL5, like other chemotactic cytokines, can bind to its specific receptors in monomeric or multimeric form. CCL5 has a dimeric structural basis, forming multimers at high concentrations, and often exists as monomers after endocytosis. Glycosaminoglycans (GAGs) on the cell surface can lead to the multimerization of CCL5. Multimerized CCL5 can not only activate inflammatory cells, prolong and aggravate inflammatory responses, but also regulate cell growth and differentiation. When CCL5 is monomeric or dimeric or its concentration is nanomolar, its receptors are CCR1, CCR3, CCR4 and CCR5, which have chemotactic activity, can enhance cytotoxicity and inhibit HIV replication. The multimeric form or micromolar level of CCL5 mainly leads to the activation of T cells, monocytes and macrophages by binding with gags on the cell surface, and enhances HIV replication.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eCCL5 has chemotactic effects on a variety of leukocytes: 1) monocytes; 2) Chemotaxis of unstimulated cd4+cd45ro+ memory T cells and activated cd4+ and cd4+t cells; 3) Chemotaxis eosinophils and stimulates their degranulation and respiratory burst; 4) It is a strong chemotactic agent for basophils, but its effect on stimulating histamine release is weak; 5) Pretreatment of T cells can increase their ability to adhere to endothelial cells stimulated by IL-1. These properties make CCL5 play an important role in immune regulatory function and autoimmune disorders and inflammatory processes.\u003c\/p\u003e\n\u003cp\u003eAs a chemokine, CCL5 not only participates in inflammatory response and mediates leukocyte migration and infiltration, but also regulates cell growth and differentiation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153465931,"sku":"abs520014-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3f05d7bbeea24687af9d513ce3983998.jpg?v=1792756822"},{"product_id":"gm-csf-elisa-kit","title":"Mouse GM-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.75pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti mouse GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, Serum\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspgm-csfdetection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003eto be improved\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81-500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e84-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreservation Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520015.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520015.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGM-CSF (granulocyte macrophage colony stimulating factor)Induce the development of monocytes, neutrophils, eosinophils, myeloid dendritic cells and dermal dendritic cells. It also acts as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. GM-CSF signaling is mediated through the receptor complex, which is composed of GM-CSF R \u0026amp; amp; Alpha; And beta C subunit, syndecan-2 as a potential co receptor. The beta C subunit is shared by the receptor complex of IL-3 and IL-5.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153498699,"sku":"abs520015-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2365d8c6008445ea9dec2abffd0e6a52.jpg?v=1792756825"},{"product_id":"ccl2-elisa-kit","title":"Mouse CCL2\/JE\/MCP-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.9pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti mouse ccl2\/je\/mcp-1 monoclonal antibody is coated on the microplate. Ccl2\/je\/mcp-1 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse ccl2\/je\/mcp-1 polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eTest Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eTest Sample Type: \u0026lt;\u003c\/strong\u003ecell supernatant, Serum, plasma\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSample loading: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003ePrecoated 96well plates, standards, anti mouse  ccl2\/je\/mcp-1one copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003eSee the instructions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e3.91-250 pg\/ml\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e84-110%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSaving Method: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e2-8\u003c\/span\u003e℃\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520016.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003cbr\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCCL2\/je\/mcp-1 is a chemokine that binds to the receptor CCR2 and induces monocyte chemotaxis. It induces the activation of monocytes, NK cells, lymphocytes and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to the site of inflammation contributes to atherosclerosis, polydactylySclerosis and the severity of allergic asthma disease. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153531467,"sku":"abs520016-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/29bdc8f2b3e7439086b968e7807a5eec.jpg?v=1792756826"},{"product_id":"il-8-elisa-kit","title":"Mouse CXCL1\/KC (IL-8) ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.09pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti mouse cxcl1\/kc monoclonal antibody is coated on the microplate. Cxcl1\/kc in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse cxcl1\/kc polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspcxcl1\/kca copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003esee the instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e84-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520017.jpg\" width=\"\" alt=\"\" height=\" 190 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520017.jpg\" alt=\"\" width=\"240\" height=\"190\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCXCL1 (GRO alpha) is a member of the CXC chemokine family. CXCL1 contains an ELR motif and preferentially attracts neutrophils. Human CXCL1 is post translationally modified and divided into three isoforms, known as CXCL1 (4-73), CXCL1 (5-73), and CXCL1 (6-73). These isoforms are produced by proteolytic cleavage after secretion by peripheral blood mononuclear cells, and their in vitro activities are higher than those of the full-length protein. The other two CXC proteins cxcl2 (or gro beta) and cxcl3 (or gro\u0026amp; gamma;)It has 90% and 86% amino acid sequence homology with CXCL1, respectively. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes, and osteocytes. In addition, CXCL1 mRNA has been detected in breast fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth regulatory gene that is constitutively overexpressed in tumorigenic cells. As a result, elevated levels of CXCL1 can be found in several types of tumors and cancers, including lung cancer and melanoma.\u003cbr\u003eCXCL1 is a member of the CXC family of chemokines. Chemokines play a role in normal and pathological processes, including anaphylaxis, angiogenesis, inflammation, tumor growth, and metastasis. Mouse cxcl2 and cxcl3 share 67% and 60% sequence homology with mouse CXCL1, respectively. In addition, rat CXCL1 has 89% homology with mouse CXCL1.\u003c\/p\u003e\n\u003cp\u003eHomologues of IL-8 have long been identified in other species, such as sheep (Yoshimura and Johnson, 1993), rabbits (Yoshimura and yuhki, 1991), pigs (Goodman et al., 1992) and dogs (Ishikawa et al., 1993), but so far, homologues of IL-8 protein in mice and rats have not been clearly defined. At present, MIP-2 (ccl8) and KC (CXCL1) are widely believed to be the functional homologs of IL-8 protein in mice\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518154186827,"sku":"abs520017-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/32fdd8681a204baea89aea34a6c6bf3c.jpg?v=1792756829"},{"product_id":"cxcl2-elisa-kit","title":"Mouse CXCL2\/MIP-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse cxcl2\/mip-2 antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, cxcl2\/mip-2 in the sample is combined with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspcxcl2\/mip-2one copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003esee the instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003cstrong\u003e96-103%\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520018.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520018.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eChemokine ligand 2 (cxcl2) is a small cytokine belonging to the CXC chemokine family, also known as macrophage inflammatory protein 2-\u0026amp; alpha; (mip2 alpha), growth regulatory protein \u0026amp; amp; beta; (GRO beta) and gro oncogene - 2 (gro-2), cxcl2 \/ gro beta, also known as MIP-2 in mice and cinc-3 in rats, is a member of the CXC chemokine family. Cxcl2 has 90% homology with CXCL1 in amino acid sequence. This chemokine is secreted by monocytes and macrophages and has a chemotactic effect on polymorphonuclear leukocytes and hematopoietic stem cells. The gene for cxcl2 is located on human chromosome 4 in other CXC chemokine clusters. Cxcl2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.\u003cbr\u003eThe length of human cxcl2 \/ gro beta is 107 amino acids (AA) and the estimated molecular weight is 11 kDa. The mouse and rat orthologs share 70% and 71% amino acid sequence identity with the human protein, respectively. The N-terminal amino acids 1-4 of cxcl2 \/ gro beta can be post translationally cleaved, thereby enhancing hematopoietic biological activity. Cxcl2 \/ gro beta is produced by a variety of cell types, including monocytes and macrophages at the site of inflammation, and has chemotactic effects on granulocytes, including neutrophils.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518154612811,"sku":"abs520018-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/dcb38131440f4c16a555538287a6cff6.jpg?v=1792756832"}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-elisa-kits.oembed?page=4","provider":"AntBio","version":"1.0","type":"link"}