{"title":"Rat ELISA Kits","description":null,"products":[{"product_id":"rat-il-2-elisa-kit","title":"Rat IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL). \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"399\" height=\"224\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240313\/df8f669701d44968a318ad6bb3e01849.jpg\" alt=\"\" width=\"300\" height=\"234\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of rat IL-2 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 84 to 107%, with an average recovery of 95%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-2 in rats was generally less than 3.5pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Calibration: The ELISA kit was calibrated with recombinant rat IL-2 protein expressed in E. coli. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of rat IL-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e95-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e96-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e97-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e99-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect both native and recombinant rat IL-2 protein.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 7.04137%; height极速赛车稳赚技巧\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-rat IL-2 antibody was precoated on a high-affinity microplate. Enzyme label plate hole with standard substance, the sample under test and biotinylated antibodies detection, after incubation, IL - 2 that exist in the sample combined with solid phase antibody and detection antibody, immune complex. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eRat  IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eRat  IL-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eRat  IL-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. \"Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor.\" Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25\/IL-2Rα+Treg cells. Notably, IL-2 is now recognized as essential for successful induction of recall responses in CD8+ memory cells. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. Two of these subunits can bind ligands and are termed IL-2Rα and IL-2Rβ. IL-2Rα is 55kDa and binds IL-2 with low affinity. Il-1β, 75kDa, is also a component of the IL-15 receptor and binds IL-2 with moderate affinity. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). γc shares receptors with IL-4, -7, -9, -15 and -21. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313507403,"sku":"abs530005-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7965d16116ec4bd894b64ab2348f3c20.jpg?v=1792753310"},{"product_id":"rat-il-4-elisa-kit","title":"Rat IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.91pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-rat IL-4 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-4 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eS\u003cspan style=\"color: #000000;\"\u003eample \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e  2.3 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1,000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e 75-112%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/57eea580c2d3435db8e80b0ee9cfefe3.png\" alt=\"\" width=\"600\" height=\"405\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: IL-4\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIl-4 is a secretory protein of 129 amino acids.Il-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils.By acting on a variety of cell types, IL-4 regulates a variety of immune responses. Il-4 is involved in several B cell activation processes and induces class II MHC molecules to be expressed in dormant B cells.It is also a common irritant in DNA synthesis. Il-4 can enhance the secretion and cell surface expression of IgE and IgG1.It also regulates the expression of low affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes.Il-4 positively regulates the expression of IL31RA in macrophages.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical hints: \u003c\/strong\u003eFor research use only, not for in vitro diagnosis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313572939,"sku":"abs530006-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d94654b6a2174d47a3631488a6cbab93.jpg?v=1792753308"},{"product_id":"rat-il-6-elisa-kit","title":"Rat IL-6 ELISA  Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable tip \u003cbr\u003e3. Distilled or deionized water \u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 8000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 8000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240111\/b1361d91d1f94ed28d56f04effb5904e.png\" alt=\"\" width=\"434\" height=\"252\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5. Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6. Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7. Repeat th 5 Step washing operation; \u003cbr\u003e8. Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9. Repeat th 5 Step washing operation; \u003cbr\u003e10. Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Four-parameter logic using computer software (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240111\/e6f5ca75fa494b62b30c679050174f04.jpg\" alt=\"\" width=\"343\" height=\"267\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels were spiked into cell culture medium samples Rat IL-6 The recovery rate was determined. The recoveries range from 82-110% , the average recovery was in 94% 。 \u003cbr\u003e2. Sensitivity: Rat IL-6 The lowest measurable dose ( MDD ) is generally less than 1.8pg\/mL 。 The lowest measurable value is determined according to 20 \u003cbr\u003e3. Linearity: 4 High concentrations of different samples were incorporated into Rat IL-6 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e4. ELISA Method can detect natural and recombinant Rat IL-6 1× ) formulated into 50ng\/mL Concentration of to detect the correlation with Rat IL-6 Cross-reactivity of. Will 50ng\/mL Rat IL-6 In the reference substance, to detect the Rat IL-6 \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 69.8827%; height: 76px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant rat protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eCNTF\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant porcine protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. No color development after substrate addition (blank well)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible causes\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, extend the incubation and substrate development times.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eDuring plate washing, the soak time was too long, the number of wash cycles was excessive, or the wash force was too strong.\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eFollow the instructions for use; do not dilute arbitrarily.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height极 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine (TMB)\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eFailing to change the pipette tip between samples, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 极 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eIdentify the suspected infectious agent and apply the appropriate handling procedures\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat IL-6 antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell stimulation factor-2, B-cell differentiation factor, BSF2, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, IL-6B-cell stimulation factor 2, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A, Rat interleukin 6 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px; text-align: center;\"\u003eRat IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px; text-align: center;\"\u003eRat IL-6 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px; text-align: center;\"\u003eRat IL-6 Detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px; text-align: center;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px; text-align: center;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px; text-align: center;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px; text-align: center;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px; text-align: center;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px; text-align: center;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Interleukin -6  IL-6 ) is a system with α A spiral structure, 22-28kDa It plays an important role in the acute phase of disease, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. A mature person IL-6 have 183 Amino acids, with mice and rats IL-6 have 41% Homology of. IL-6 Internal alternative splicing results in a variety of isomers, some of which exhibit antagonistic properties. Known expression IL-6 The cells comprise CD8+T Cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical nerve cells, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B  β Cells. IL-6 The production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids and is generally associated with cellular activation. In normal human blood IL-6 In 1pg\/mL Within the range, there is a slight increase in menstrual period, a moderate increase in some cancers, and a significant increase after surgery. \u003cbr\u003eIL-6 Cellular signaling is initiated by a cell surface heterodimeric receptor complex consisting of a ligand-binding subunit \t （ IL-6 Receptor) and a signal transduction subunit gp130 Composition. IL-6 Binding to IL-6  IL-6 Receptor with gp130 Combination of and gp130 Dimerization of. gp130 Also CLC  CNTF 、 CT-1 、 IL-11  IL-27 、 LIF And OSM  IL-6 Receptors are produced by alternative splicing and proteolysis. Through a trans-signaling mechanism, soluble IL-6  IL-6 Receptor complexes can trigger surface deficiency IL-6  gp130 Response of cells. IL-6  gp130 Molecular expression is very broad, and trans-signaling enables a wider range of cell type pairs IL-6 Response of. Soluble gp130 The spliceosome prevents IL-6\/IL-6R Trans-signal transduction, but does not prevent other cytokine utilization gp130 Signal transduction of molecules as co-receptors. \u003cbr\u003eIL-6 With tumor necrosis factor ( TNF ) and IL-1 The acute inflammatory reaction caused by it plays an almost unique role in fever and acute inflammatory reaction of the liver, and it also plays an important role in the transformation of acute inflammation into acquired immunity or chronic inflammatory diseases. IL-6 Disorders contribute to chronic inflammation in cases such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving IL-6  TGF- Under the conditions of existence, IL-6 In the naive type T Cells to Th17 It plays an important role in the process of inflammatory cell differentiation. IL-6 Modulates bone resorption, and by promoting Th17 The active component of inflammatory cells is the main factor causing inflammatory joint damage in rheumatoid arthritis I 。 L-6 Contributes to the formation and instability of atherosclerotic plaque. but IL-6 It also has anti-inflammatory effects, such as skeletal muscle secretion during physical exercise IL-6 。 It acts as a hematopoietic stem cell growth factor to promote hematopoiesis, induction B The cells mature into plasma cells, immortalized multiple myeloma cells. IL-6 It also promotes but initiates the development of other inflammation-related cancers, such as enteritis-related cancers. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, store at 2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e125pg\/mL-8000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313704011,"sku":"abs530003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a58cf7bed63649579d16b1ae2c1666cd.jpg?v=1792756807"},{"product_id":"rat-il-1f2-elisa-kit","title":"Rat IL-1β\/IL-1F2 ELISA  Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength)\u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable tip\u003cbr\u003e\u003cbr\u003e3. Distilled or deionized water\u003cbr\u003e\u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\u003cbr\u003e5. 500mL\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1×\u003cbr\u003e\u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1×\u003cbr\u003e\u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution.\u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ）\u003cbr\u003e\u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL\u003cbr\u003e\u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration.\u003cbr\u003e\u003cbr\u003e④SA-HRP SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1×\u003cbr\u003e\u003cbr\u003e⑤ Color development solution: per well 100uL\u003cbr\u003e\u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× 1000uL , obtaining a concentration of 4000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× 4000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"436\" height=\"245\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all necessary reagents and standards.\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal;\u003cbr\u003e\u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e\u003cbr\u003e5. Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3\u003cbr\u003e\u003cbr\u003e6. Within each well added 100uL 2 Hour;\u003cbr\u003e\u003cbr\u003e7. Repeat th 5 Step washing operation;\u003cbr\u003e\u003cbr\u003e8. Within each well added 100uLSA-HRP 20 Minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat th 5 Step washing operation;\u003cbr\u003e\u003cbr\u003e10. Within each well added 100uL 5-30 Minutes, pay attention to avoiding light;\u003cbr\u003e\u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240724\/79cd22cb0cf74cb7bbcf331e91abae93.jpg\" alt=\"\" width=\"436\" height=\"245\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels were spiked into cell culture medium samples Rat IL-1β\/IL-1F2 The recovery rate was determined. The recoveries range from 82-110% , the average recovery was in 94% 。\u003cbr\u003e\u003cbr\u003e2. Sensitivity: Rat IL-1β\/IL-1F2 The lowest measurable dose ( MDD ) is generally less than 1.8pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve.\u003cbr\u003e\u003cbr\u003e3. Linearity: 4 High concentrations of different samples were incorporated into Rat IL-1β\/IL-1F2 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA Method can detect natural and recombinant Rat IL-1β\/IL-1F2 Egg whites. The following factors were mixed with diluent ( 1× ) configured to 50ng\/mL Concentration to be detected with rats IL-1β Cross-reactivity of. Will 50ng\/mL Interference factors incorporated into the intermediate range of recombinant rats IL-1β In the control article, to detect the effect on rats IL-1β Of interference. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.5346%; height: 143px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant rat protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RI\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RAcP\/Fc Chimera\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 11px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 11px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 11px;\"\u003eIL-1Rrp2\/Fc Chimera\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (no color appears after color rendering is complete)\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher)\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat IL-1beta\/IL-1F2 antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IL-1beta\/IL-1F2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ecatabolin, IL1 beta, IL-1 beta, IL-1, IL1B, IL-1b, IL1-beta, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1 beta, Rat interleukin 1β Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use within the expiration date of the kit \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 281px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px; text-align: center;\"\u003eRat IL-1β\/IL-1F2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 44px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px; text-align: center;\"\u003eRat IL-1β\/IL-1F2 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 16px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px; text-align: center;\"\u003eRat IL-1β\/IL-1F2 Detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 16px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 11px; text-align: center;\"\u003e40× SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 11px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 11px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px; text-align: center;\"\u003e10× dilution buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 115px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px; text-align: center;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 24px; text-align: center;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 24px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px; text-align: center;\"\u003e20× wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px; text-align: center;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 35px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Interleukin -1 （ IL-1 ) Protein family members include classical IL-1 And IL-1 。 IL-1 Receptor antagonists ( IL-1RA ）、 IL-18 、 IL-33 、 IL-1F5-F10 、 IL-1 And IL-1 Have the same cell surface binding receptors and form the same biological function. Unstimulated cells in healthy people do not produce IL-1 The only exceptions are skin keratinocytes, some epithelial cells, and some cells of the central nervous system. But in response to inflammatory agents, infection, or microbial endotoxin, IL-1 Expression in macrophages and other types of cells increased dramatically. IL-1 In immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, GH\/IGF-1 Plays an important role in the physiological process of. Multiple diseases and IL-1 Dysregulated expression or delayed expression associated, including diseases related to sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury and senile degeneration. \u003cbr\u003e IL-1 And IL-1 Are structurally related polypeptides, at the amino acid level about 25% Homology of. Both are first synthesized into 31kDa Precursor, then cleaved to approximately 17.5kDa Of the mature protein. By Caspase-1\/ICE Right IL-1 Cleavage of precursors is a critical step in the inflammatory response. Despite IL-1 And IL-1 Neither contain a typical hydrophobic signal peptide, but there is evidence that they can be secreted extracellularly through non-classical pathways. Unprocessed IL-1 Parts may be displayed on the cell membrane and may retain biological activity. With IL-1 The precursors are different, IL-1 Precursors vs. mature IL-1 Compared to having little or no biological activity. IL-1 Precursors vs. mature IL-1 All transported extracellularly. \u003cbr\u003e IL-1 And IL-1 Via immunoglobulin superfamily receptors and IL-1RA Combine to exert its effect. 80kDa Of I Type transmembrane receptor ( IL-1RI ) expressed in a variety of cells, including T Cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes. 68kDa Of II Type transmembrane receptor ( IL-1RII ) in B Cells, neutrophils and bone marrow cells. IL-1RI And IL-1RII The extracellular region of 28% Homology of. But there are significant differences in other areas: II The intracellular domain of type receptor is only 29 Amino acids, and I The intracellular domain of type receptor is 213 Amino acids. IL-1RII It seems to be right IL-1 The signal does not respond, its function is to decoy the receptor and thus weaken IL-1 The role of. IL-1 Receptor ligand protein ( IL-1RAcP ) with IL-1RI The interaction is IL-1RI Necessary for signaling. IL-1RA Is a secreted molecule, is IL-1 Competitive inhibitors. Soluble IL-1RI And IL-1RII It is present in human plasma, joint fluid, and conditioned media of several cell lines. Moreover, vaccinia virus encodes similar to soluble IL-1RII Of IL-1 Binding proteins. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, store at 2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518155333707,"sku":"abs530002-96T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rat-tnf-a-elisa-kit","title":"Rat TNF-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable tip \u003cbr\u003e\u003cbr\u003e3. Distilled or deionized water \u003cbr\u003e\u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e\u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e\u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e\u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e\u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e\u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e\u003cbr\u003e⑤ Color developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The color developer removed is for use on the same day only. \u003cbr\u003e\u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 4000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 4000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231227\/e3099c13fee94dfb81d170f42fac0d25.png\" alt=\"\" width=\"434\" height=\"244\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e\u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e\u003cbr\u003e5. The liquid in the plate is sucked off and the plate is washed using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e\u003cbr\u003e7. Repeat th 5 Step washing operation; \u003cbr\u003e\u003cbr\u003e8. Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat th 5 Step washing operation; \u003cbr\u003e\u003cbr\u003e10. Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e\u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231227\/1f6b993a5cce4887a6f874f656940c91.jpg\" alt=\"\" width=\"339\" height=\"264\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery: Different levels were spiked into cell culture medium samples Rat TNF-α The recovery rate was determined. The recoveries range from 75-112% , the average recovery was in 90% 。 \u003cbr\u003e\u003cbr\u003e2. Sensitivity: Rat TNF-α The lowest measurable dose ( MDD ) is generally less than 7.8pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e\u003cbr\u003e3. Correction: This ELISA High-purity recombinant kit expressed by Escherichia coli Rat TNF-α Corrected by protein. \u003cbr\u003e\u003cbr\u003e4. Linearity: 4 High concentrations of different samples were incorporated into Rat TNF-α , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity.\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eAverage \/ Expected value ( % ）\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange ( % ）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e96-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e109\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-116\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e98-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA Method can detect natural and recombinant Rat TNF-α Egg whites. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eNumber\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height极速赛车7码公式\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Species Reactivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Rat\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat TNF-α antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt; Please use it within the validity period of the kit (new and old products are shipped randomly) \u0026lt;\/strong\u0026gt;\u0026lt;br \/\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eRat TNF-α Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eRat TNF-α Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eRat TNF-α Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototype ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-α is also implicated in a number of pathological conditions, including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmune diseases, and cancer. Rat TNF-alpha is a 26kDa type II transmembrane protein, which consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane fragment, and a 156 aa extracellular domain (ECD). In ECD, rat TNF-alpha has 95% amino acid sequence identity with mouse and 73%-79% amino acid sequence identity with bovine, canine, cotton rat, horse, cat, human, macaque, and pig TNF-alpha. It is produced by a variety of immune cells, epithelial cells, endothelial cells and tumor cells. TNF-α assembles intracellularly to form non-covalently linked homotrimers expressed on the cell surface. Cell surface TNF-α can not only induce lysis of tumor cells and virus-infected cells, but also produce its own downstream cell signaling after ligation through soluble TNFRI. TACE\/ADAM17 releases membrane-bound TNF-α to release a bioactive cytokine, which is a 55 kDa soluble trimer of the extracellular domain of TNF-α. TNF-α binds to the ubiquitous 55-60 kDa TNFRI and the hematopoietic cell-restricted 78-80 kDa TNFRII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-α with comparable affinity and can promote NFκB activation. However, only TNF RI contains a cytoplasmic death domain, which triggers the activation of apoptosis. Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-α.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent (1 ×) in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518155857995,"sku":"abs530007-96T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rat-ifn","title":"Rat IFN-γ ELISA KIT","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti rat \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003eThe monoclonal antibody was coated on the microplate, and \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e was added; Polyclonal antibody, and \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e bound to the microplate; Binding, free components are washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plates, standards, anti rat IFN - γ One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e15.6 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e39.1 - 2500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e89.0-118.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃  span\u0026gt;\u003c\/p\u003e\n\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003cp\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20200827\/fc85552aa7ec48eca11523a6959f1648.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIFN-γ (interferon -γ)It is a typical proinflammatory cytokine produced by a variety of immune cells under inflammatory conditions, especially by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and immunoglobulin class switching in B cells. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ Acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. Ifn-γ Dimers pass through two ifn-γ R1 and two ifn-γ The receptor complex of the R2 subunit signals.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":43115011309643,"sku":"abs530004-96T","price":450.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-elisa-kits-rat-elisa-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}