{"title":"Porcine ELISA Kits","description":null,"products":[{"product_id":"porcine-il-6-elisa-kit","title":"Porcine IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.1pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti-porcine IL-6 antibody was pre coated on a high affinity microplate. Standards, samples to be tested and biotinylated detection antibodies are added into the wells of enzyme plate. After incubation, IL-6 in the samples combines with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003epre coated 96 well plate, standard, anti-porcine IL-6 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8  Pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e125 - 8000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e70-114%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191227\/10e0016ad35d4564a34397548ab2faa4.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin-6 (IL-6)Is a α Helical structure, 22-28kd phosphorylation and different degrees of glycosylation of multifunctional cytokines, which play an important role in the acute phase of disease reaction, inflammation, hematopoiesis, bone metabolism and cancer deterioration. Mature mouse IL-6 has 187 amino acids and has 42% and 85% homology with human and rat IL-6, respectively. The alternative splicing of IL-6 generates a variety of isoforms, some of which show antagonistic properties. Cells known to express IL-6 include cd8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cellsFetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islets beta; Cells. IL-6 production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids, and is generally associated with cell activation. IL-6 in the blood of normal people is in the range of 1 pg\/ml, which increases slightly during menstruation, moderately in the middle and late stages of some cancers, and significantly after major surgery.\u003cbr\u003e\u003cbr\u003eIL-6 triggers cell signals through cell surface receptors, which are heterodimeric complexes composed of ligand binding subunit (IL-6 receptor) and signal transduction subunit gp130. IL-6 binds to the IL-6 receptor, triggering the binding of IL-6 receptor to gp130 and the dimerization of gp130. Gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptor is produced by alternative splicing and protease cleavage. Through the trans signal transduction mechanism, the complex of soluble IL-6 and IL-6 receptor can trigger the response of cells lacking IL-6 receptor on the surface but expressing gp130. The expression of IL-6 receptor is mainly limited to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Due to the wide expression of gp130 molecule, trans signal transduction realizes the response of a wider range of cell types to IL-6. The soluble gp130 spliceosome prevented the trans signal transduction of il-6\/il-6r, but could not prevent the signal transduction of other cytokines using gp130 molecule as a co receptor.\u003cbr\u003e\u003cbr\u003eIL-6 and tumor necrosis factor alpha; (tnf-α)Together with IL-1, the acute inflammatory response caused by IL-1 plays an almost unique role in fever and liver acute inflammatory response. It also plays an important role in the transformation from acute inflammation to acquired immunity or chronic inflammatory diseases. Dysregulation of IL-6 can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis and sepsis, often involving the trans signal transduction of IL-6. In transforming growth factor TGF - β In the presence of IL-6, IL-6 plays an important role in the differentiation of naive T cell phase Th17 inflammatory cells. IL-6 regulates bone resorption and promotes the activity of Th17 inflammatory cells, which is the main factor causing inflammatory joint injury in rheumatoid arthritis. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects. For example, skeletal muscle secretes IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes hematopoiesis, induces B cells to mature into plasma cells, and the immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation related cancers, such as colitis related cancers.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293313441867,"sku":"abs540003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/835d962b9d3648ca9f4cb34af1f7e82f.jpg?v=1792753355"},{"product_id":"porcine-ifn-gamma-elisa-kit","title":"Porcine IFN-γ ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.75pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. Specific anti porcine IFN - \u0026amp; gamma; The antibody was pre coated on a high affinity microplate. Add standards, samples to be tested and biotinylated detection antibodies into the wells of the enzyme plate. After incubation, ifn-\u0026amp;gamma in the samples; Combine with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003epre coated 96 well plate, standard, anti porcine IFN gamma detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e7.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range: \u003c\/strong\u003e62.5 - 4000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery range: \u003c\/strong\u003e90-116%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191227\/cf1ab8cff1334192861bfec46496e98b.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eγ interferon (also known as type II interferon)It is an important cytokine that can exercise immune regulatory function. It was discovered for its antiviral activityΓ Interferons play a key role in host defense through antiviral, antiproliferative and immunomodulatory functions. In many types of cells, γ Interferon can induce the production of cytokines and upregulate the expression of different membrane proteins, including type i\/ii major histocompatibility complex (MHC-I, MHC-II), Fc receptors, leukocyte adhesion molecules, B7 family antigensΓ Interferon can effectively activate macrophages and guide the synthesis, type transformation and secretion of B cell immunoglobulinΓ Interferon can also affect the development of T helper cell subtypes by inhibiting Th2 differentiation and stimulating the growth of Th1Γ Interferons play an important role in some inflammatory diseases such as autoimmunity and atherosclerosis.\u003cbr\u003e\u003cbr\u003ebioactive gamma; Interferon is a non covalently bound dimer, with a molecular weight of about 20-25kd and varying degrees of glycosylation. Mature people γ Interferon and gamma of rhesus monkey; The amino acid sequence homology of interferon is close to 90%, which is similar to gamma of cattle, dogs, horses, cats and pigs; Interferon has 59-64% homology with gamma of cotton mouse, mouse and rat; Interferon homology is 37-43%. Interferon dimers first interact with the transmembrane receptor IFN - γ RI (α subunit) binds to ifn-γ RII (β subunit) combines to form a α Subunit and two β A functional complex of subunits; Ifn-\u0026amp;gamma in the complex receptor; RII can increase the affinity of ligands as well as signal transduction efficiency. Although γ Subunit is constitutively expressed in many types of cells, but β Gamma, a regulatory receptor expressed by subunit cells; The effect of interferon response status and is strictly regulated\u003cbr\u003e\u003cbr\u003eγ Interferon can be secreted by many cells under a series of inflammatory conditions, including dendritic epidermis \/ gammaΔ T cells, keratinocytes, peripheral blood gammaΔ T cells, mast cells, neurons, cd8+t cells, macrophages, B cells, neutrophils, natural killer cells, cd4+t cells and testicular sperm cells, etc\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518155923531,"sku":"abs540001-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/fce4f8d1d93b4f58b2bb9946aec9a54b.jpg?v=1792753349"},{"product_id":"porcine-il-1-beta-elisa-kit","title":"Porcine IL-1β ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.52pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. Specific anti porcine IL-1-beta; The antibody was pre coated on a high affinity microplate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of the enzyme plate. After incubation, the il-1\u0026amp;beta in the sample; Combine with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plate, standard, anti porcine il-1β One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e3.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e62.5 - 4000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e69-102%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191227\/0b5bc82ee99e49b5b6d7a6186d188ded.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 1 (IL-1)The protein family contains the canonical members il-1\u0026amp;alphaIl-1β And IL-1ra, as well as IL-18, IL-33, and il-1f5-10. Il-1α And il-1β It binds to the same cell surface receptors and shares biological functions. In addition to skin keratinocytes, some epithelial cells, and some cells of the central nervous system, unstimulated cells in healthy mice do not produce IL-1. However, as a response to inflammatory substances, infection or microbial endotoxin, macrophages and many other cells will produce IL-1 in large quantities. In the physiological process of immune response and inflammatory response, bone remodeling, fever, glucose metabolism, growth hormone \/ IGF-1, il-1β Plays a key role. Under a series of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury and aging related diseases, IL-1 has been accompanied by abnormal or continuous production.\u003cbr\u003e\u003cbr\u003eIL-1α And il-1β It has structurally related polypeptides with approximately 25% homology at the amino acid level. Both of them were synthesized as 31 kDa sized precursors and then cleaved into mature proteins with a molecular weight of approximately 17.5 kDa. Caspase-1 \/ ICeI cleaves il-1β Precursors are a key step in the inflammatory response. Il-1α And il-1β None of them contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through non canonical pathways. A portion of unprocessed il-1α It can appear on the cell membrane and may have biological activity. With il-1α Different precursors, il-1β The precursors have little biological activity compared with the mature ones. Unprocessed and mature il-1β Can be exported from cells.\u003cbr\u003e\u003cbr\u003eIL-1α And il-1β Functions through immunoglobulin superfamily receptors that bind to IL-1ra. Cells expressing 80kDa transmembrane type I receptor (IL-1 RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid inner layer cells, chondrocytes, and hepatocytes. Cells expressing 68kDa transmembrane type II receptor (IL-1 RII) include B cells, neutrophils, and bone marrow cells. The extracellular domains of these two IL-1 receptors share about 28% homology, but the significant difference is reflected in their intracellular domains: the intracellular domain of type II receptor has only 29 amino acids, while the intracellular domain of type I receptor has 217 amino acids. IL-1 RII does not produce signal for IL-1, and may act as a decoy receptor to weaken IL-1 function. IL-1 receptor accessory protein (IL-1 RACP) is associated with IL-1 RI and is required for IL-1RI signal transduction. IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1 RII can be detected in human plasma, synovial fluid and conditioned medium of various human cell lines. In addition, IL-1 binding protein similar to soluble IL-1 RII is able to be encoded by vaccinia and vaccinia virus.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518155989067,"sku":"abs540002-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/91fd464e47844a6a9a5a10e60f3541d7.jpg?v=1792753354"}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-elisa-kits-porcine-elisa-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}