{"title":"Mouse ELISA Kits","description":null,"products":[{"product_id":"mouse-il-7-elisa-kit","title":"Mouse IL-7 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable tip \u003cbr\u003e3. Distilled or deionized water \u003cbr\u003e4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes at a rotational speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2. Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Color development solution: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of chromogenic solution, and protect it from light; The chromogenic solution removed is for the same day only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 2000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 2000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240429\/63129bafba5843deb0701ba8358dde24.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2. Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3. Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4. Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5. The liquid in the plate is sucked off and the plate is washed using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6. Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7. Repeat th 5 Step washing operation; \u003cbr\u003e8. Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9. Repeat th 5 Step washing operation; \u003cbr\u003e10. Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11. Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12. After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13. Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Four-parameter logic using computer software (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241114\/e2e4d02a892946e48b8be03b5a091360.jpg\" alt=\"\" width=\"369\" height=\"286\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: Mouse IL-7 The lowest measurable dose ( MDD ) is generally less than 1.7pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e2. Correction: This ELISA High-purity recombinant kit expressed by Escherichia coli Mouse IL-7 Corrected by protein. \u003cbr\u003e3. Linearity: 6 High concentrations of different samples were incorporated into Mouse IL-7 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e4. Specificity: This ELISA Method can detect natural and recombinant Mouse IL-7 Egg whites. The following factors were mixed with diluent ( 1× ) configured to 50ng\/mL Concentration of to detect the correlation with Mouse IL-7 Cross-reactivity of. Will 50ng\/mL Of interfering factors incorporated into intermediate range recombination Mouse IL-7 In the reference substance, to detect the Mouse IL-7 Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eIL-7 Rα\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eIL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eTSLP\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eHemolysis, storage for too long, incomplete agglutination, contamination by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse IL-7 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-7 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL7, IL-7, IL-7interleukin-7, interleukin 7, Lymphopoietin-1, PBGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-7 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eMouse IL-7 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eMouse IL-7 Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-7 (Interleukin-7) is a cytokine that plays an important role in lymphocyte differentiation, proliferation and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestine. It signals through a receptor complex consisting of IL-7R alpha\/CD127 and Common γ Chain. γc is also a subunit of the IL-2,-4,-9,-15 and-21 receptors. IL-7 helps to maintain all primitive and memory T cells. Furthermore, IL-7 is required for T cell-dendritic cell interactions. In mice, IL-7 activation of IL-7Ralpha is essential for the development of both T cells and B cell lineages, whereas in humans T cells are not required, whereas B cell development is required.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311082571,"sku":"abs520020-96T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-il-2-elisa-kit","title":"Mouse IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\u003cbr\u003e5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/9297927b5c1b4d6dbdde6fd6fcaad53d.png\" alt=\"\" width=\"433\" height=\"245\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240201\/84b805a141c641e18449b8ec36ac948f.jpg\" alt=\"\" width=\"301\" height=\"235\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery rate: Different levels of Mouse IL-2 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 84 to 107%, with an average recovery of 95%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: Mouse IL - 2 the minimum detectable dose (MDD) is generally less than 3.5 pg\/mL. Minimum measurable value is according to the 20 standard curve zero absorbance value plus two times the standard deviation of average calculated corresponding to the concentration.\u003cbr\u003e\u003cbr\u003e3. Correction: the ELISA kit by e. coli expressed high purity recombinant Mouse IL - 2 protein correction.\u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse IL-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e96-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e97-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e99-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: Both native and recombinant Mouse IL-2 protein could be detected by this ELISA. The following factors were diluted (1×) Configure to a concentration of 50ng\/mL to detect cross-reactivity with Mouse IL-2. To detect interference with Mouse IL-2, 50ng\/mL of interfering factor was incorporated into the intermediate range of recombinant Mouse IL-2 controls. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e IL-9\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e IL-15\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rβ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车5码公式\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-2 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-2 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. \"Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor.\" Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25\/IL-2Rα+Treg cells. It is important to note that now IL - 2 is considered successful induction of CD8 + memory cells necessary for memory response. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. The two subunits can be combined with ligands, called IL - 2 r alpha and beta IL - 2 r. IL-2Rα is 55kDa and binds IL-2 with low affinity. IL - 2 beta for 75 kda, is part of the IL - 15 receptors, moderate affinity with IL - 2. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). Gamma c and IL - 4, 7, 9, 15-21 and Shared receptors. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311180875,"sku":"abs520002-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/83ff000cd49749aaa9f0572e7394cfe2.jpg?v=1792753496"},{"product_id":"mouse-il-4-elisa-kit","title":"Mouse IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240320\/990f0778e326410e9897ab9366ec1de1.jpg\" alt=\"\" width=\"428\" height=\"333\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3, kit parameter \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of mouse IL-4 were incorporated into the cell culture medium samples and the recovery rate was determined. Recovery range in 75-112%, the average recovery rate at 90%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-4 in mice was generally less than 2.3pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 4 protein in mice. \u003cbr\u003e4. Linear: four different samples with high levels of IL - 4 in mice, and then use thinner (1 \u0026amp; times) Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.2527%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e96-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e109\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-116\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1：16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e98-105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse IL-4 protein. The following factors in diluent (1 \u0026amp; times) The concentration of the mixture 50 ng\/mL to detect and IL - 4 cross reaction in mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 4 reference substance in the restructuring of the mice, the interference of IL - 4 to test in mice. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 41.7154%; height: 82px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human proteins\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-4R\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cstrong\u003e4, common problem resolution\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车开奖官网 is a government-run official platform that provides timely and accurate lottery results. Users can check the latest winning numbers, prize distributions, and historical data to stay informed. The website ensures fairness and transparency in all lottery operations, offering a trusted source for enthusiasts. Always play responsibly and within legal boundaries.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Sensitivity\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;2.75pg\/mL\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-4 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the well of microplate. After incubation, IL-4 present in the sample combined with solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-4 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-4 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-4 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-4 (IL-4), also known as B cell stimulating factor 1, is a monomeric cytokine of about 18-20 kda that has pleiotropic effects in immune responses. The precursor of mouse IL-4 is 140 amino acids, including a 20-amino acid signal peptide and a 120-amino acid mature chain. IL-4 contains three N-linked glycosylation potential sites, three intra-chain disulphide bonds, and its structure is a bundled tetra-α-spirochetes. Similar to human IL-4, mouse IL-4 has a small selective splicer. \"Unlike human IL-4, the expression of this spliceosome is thought to be low and of little significance.\" The amino acid sequence of mature mouse IL-4 shared 43% and 63% identity with human and rat IL-4, respectively. Studies have shown that the activities of human, mouse and rat IL-4 are species specific. IL - 4 by toward Th2 CD4 + T cells, basophils and mast cells and NKT cells, gamma delta T cells with eosinophilic cells expressed. \u003cbr\u003eIL - 4 has played a key role in type 2 immune reaction, can promote the Th2 differentiation and B cell secretory immunoglobulin G1 and immunoglobulin E subtypes. IL-4 causes Th2 differentiation by binding to type I or type II receptor complexes containing IL-4 receptor alpha subunits coupled to the γ chain or IL13Rα1, respectively. IL - 4 by cell surface receptors of different dimers, activate JAK family of protein complex amino acid kinase, cause its cytoplasm C phosphorylation. This triggers the recruitment and phosphorylation of STAT6. The phosphorylation and conformational changes of STAT6 lead to dimerization, nuclear translocation, binding and transcriptional activation of STAT6 target genes, including IL-4, IL-5, IL-13, and Th2-specific cytokines GATA3 and c-Maf. On the function, IL - 4 B cell proliferation, survival, and promote mice immune globulin class into IgG1 and IgE, promote the mast cells, eosinophils, and basophils start and chemokines, promote Th2 phenotype naive CD4 + cells, and promote the epithelial cell proliferation and activation. IL-4 is also an important cytokine in tumor immunology. In early experiments in mice, the researchers found that IL-4 had potent anti-tumor abilities. Mice reject tumors expressing IL-4 and develop long-term antitumor immunity. This may be due to the antiangiogenic effect of IL-4 or its ability to activate, select for CD8+T cells. \"Paradoxically, emerging evidence suggests that IL-4 is a tumor-promoting molecule, and therefore, IL-4 is a reverse-acting cytokine.\" \"A possible explanation is that IL-4 induces upregulation of antiapoptotic molecules in tumor cells and downregulation of cytolytic molecules in CD8+T cells.\" In addition, tumor-produced IL-4 is also thought to act on local tumor-associated macrophages (TAMs) to induce the secretion of cathepsins that promote cell migration.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311246411,"sku":"abs520003-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/87e652a2480d4f0b85b4ca6747b6bdf2.jpg?v=1792753498"},{"product_id":"mouse-il-6-elisa-kit","title":"Mouse IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e①1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240109\/9ef19a3c0d9b4bd496544968c6147c31.jpg\" alt=\"\" width=\"272\" height=\"212\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Mouse IL-6 were incorporated into the cell culture medium samples and the recovery rate was determined. Recoveries ranged from 70 to 114%, with an average recovery of 91%. \u003cbr\u003e\u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of Mouse IL-6 was generally less than 1.8pg\/mL. The lowest detectable value of mouse IL-6 was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with high purity recombinant Mouse IL-6 protein expressed in E. coli. \u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse IL-6, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003ctable style=\"border-collapse: collapse; width: 56.9582%; height: 209px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1：8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e109\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e102-112\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 12px;\"\u003e1：16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%;\"\u003e117\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 12px;\"\u003e112-120\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA can detect native and recombinant Mouse IL-6 protein. The following factors in diluent mixture 50 ng\/mL (1 x) to detect the concentration and Mouse IL - 6 cross reaction. Interference with Mouse IL-6 was tested by incorporating 50ng\/mL of the interfering factor into the intermediate range of recombinant Mouse IL-6 controls. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 47.5817%; height: 32px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.49pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-6 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-6 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-6 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-6 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-6 (IL-6) is a multifunctional cytokine with α-helical structure, 22-28kD phosphorylation and different degrees of glycosylation. It plays an important role in the acute phase of disease response, inflammation, hematopoiesis, bone metabolism and cancer progression. Mature mouse IL-6 has 187 amino acids and is 42% and 85% identical to human and rat IL-6, respectively. Alternative splicing within IL-6 results in a variety of isoforms, some of which exhibit antagonistic properties. Expression of IL - 6 cells is known, \"These include CD8+T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, and neutrophils. , monocytes, eosinophils, colon epithelial cells, B1B cells and islet beta cells. The production of IL - 6 is usually in the sugar cortical hormone, catecholamine and under the control of secondary sexual steroids, generally associated with cell activation. The level of IL-6 in normal human blood is in the range of 1pg\/mL. It is slightly increased in the menstrual period, moderately increased in the middle and late stage of some cancers, and significantly increased after major surgery. \u003cbr\u003eIL-6 elicits cell signaling through a cell surface receptor, which is a heterodimeric complex consisting of a ligand-binding subunit (the IL-6 receptor) and a signal transduction subunit gp130. IL-6 binds to the IL-6 receptor, triggering the binding of IL-6 receptor to gp130 and the dimerization of gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble IL - 6 receptor is produced by alternative splicing and protease cutting. \"Through a trans-signaling mechanism, the combination of soluble IL-6 and IL-6 receptor triggers a response in cells that lack the IL-6 receptor on the surface but express gp130.\" Expression of the IL-6 receptor is largely restricted to hepatocytes, monocytes, lymphocytes, and resting lymphocytes, and since the gp130 molecule is expressed very broadly, trans-signaling enables a broader range of cell types to respond to IL-6. \"Soluble gp130 spliceosome prevented IL-6\/IL-6R trans-signaling, but not signaling by other cytokines using gp130 as a co-receptor.\" \u003cbr\u003eThe acute inflammatory response induced by IL-6, together with tumor necrosis factor-α (TNF-α) and IL-1, plays an almost unique role in fever and acute liver inflammation. It also plays an important role in the transformation from acute inflammation to acquired immunity or chronic inflammatory diseases. IL-6 dysregulation can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving IL-6 trans-signaling. In the presence of transforming growth factor TGF-β, IL-6 plays an important role in the differentiation of Th17 inflammatory cells from naive T cells. IL-6 regulates bone resorption by promoting the activity of Th17 inflammatory cells, which is the main factor causing inflammatory joint damage in rheumatoid arthritis. IL-6 is involved in the formation and destabilization of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects, such as secretion of IL-6 by skeletal muscle during physical exercise, promoting hematopoiesis as a growth factor for hematopoietic stem cells, inducing the maturation of B cells into plasma cells, and immortalization of multiple myeloma cells. Also promote IL - 6, but may not start the other inflammation associated with cancer, such as colitis associated cancer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311279179,"sku":"abs520004-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/95bc6c59708e4c14b8e6c76753199553.jpg?v=1792753504"},{"product_id":"mouse-vegf-elisa-kit","title":"Mouse VEGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL). \u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/3518f8f0e7ba44aba5337ea9784cf5c2.png\" alt=\"\" width=\"385\" height=\"218\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Ready for all the needed reagent and standard; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/51b42183e55442c5b538655dcd06491f.jpg\" alt=\"\" width=\"241\" height=\"187\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Mouse VEGF were incorporated into the cell culture medium samples and the recovery rate was determined. Recovery range in 82-110%, the average recovery rate at 94%. Sensitivity: \u003cbr\u003e\u003cbr\u003e2. The minimum measurable Mouse VEGF dose (MDD) is generally less than 1.8 pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with high purity recombinant Mouse VEGF protein expressed by Sf21. \u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of Mouse VEGF followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range. \u003cbr\u003e\u003cbr\u003e5. Specificity: both native and recombinant Mouse VEGF protein could be detected by this ELISA. The following factors in diluent (1 \u0026amp; times) Configure to a concentration of 50ng\/mL to detect cross-reactivity with mouse VEGF. Interference with mouse VEGF was detected by incorporating 50ng\/mL of the interfering factor into the intermediate range of recombinant mouse VEGF controls. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.9244%; height: 120px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF R2\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF115\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF-B167\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF165\/pIGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eVEGF-B186\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003eVEGF-D\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4, common problem resolution\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specificity of anti VEGF antibody in mice pre package is on the high affinity enzyme label plate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the VEGF present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse VEGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse VEGF standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse VEGF detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is A potent regulator of neovascularization and angiogenesis in fetuses and adults. Vascular endothelial growth factor is PDGF family, the family proteins there are eight characteristics of conservative and cystine nodes through a reverse parallel disulfide bond dimers (4). After selective splicing, VEGF spliceosome has different amino acid lengths. VEGF120, VEGF164, VEGF188 in mice; Human VEGF VEGF121, VEGF145, VEGF164, VEGF183, VEGF189 and VEGF206. VEGF165 was the most expressed and active isoform in human, followed by VEGF121 and VEGF89. The same may be true of mice. With the exception of VEGF120 and VEGF121, the other isoforms of VEGF contain the basic heparin-binding domain and do not diffuse freely. Mice VEGF164 and corresponding protein amino acid 97% homology of rats; The homology with human or porcine VEGF was 89%, with bovine VEGF 88%, and with cat, horse, and dog VEGF 90%. VEGF is expressed in a variety of cells and tissues, including skeletal muscle and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34+ stem cells, endothelial cells, cellulose cells, vascular smooth muscle cells, and so on. VEGF expression is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatinM and tumor necrosis factor-α. Expression of VEGF isoforms also varies during development and in adults. \"The dimer of \u003cbr\u003eVEGF binds to two related tyrosine kinase receptors, VEGFR1 (also called FLt-1) and VEGFR2 (Flk-1\/KDR).\" Binding of VEGF to its receptor induces homodimerization and autophosphorylation of the latter. These receptors possess seven extracellular immunoglobulin-like domains. Vascular endothelial cells and some non-endothelial cells have VEGF receptor expression. \"Although VEGF has the highest affinity for VEGFR1, VEGFR2 is the major factor regulating the angiogenic activity of VEGF.\" VEGF165 also binds to the semaphoring receptor and neuropilin-1, thereby promoting the complex formation with VEGFR2. \u003cbr\u003eVEGF is famous for its participation in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, thereby regulating the density and volume of blood vessels, but it does not play a role in the pattern of blood vessel formation. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemotactic factor. In the postpartum period, VEGF maintains the integrity of vascular endothelial cells and is a potent mitogen for large\/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and in the female reproductive cycle. In disease tissues, VEGF promote the permeability of blood vessels. Thus, VEGF is involved in the metastatic process of tumors by promoting extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control VEGF-induced tumor angiogenesis. The level of circulating VEGF correlates with the severity of autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293311508555,"sku":"abs520008-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/705eaefdf45a44128d4c3dbd11f3772d.jpg?v=1792753520"},{"product_id":"mouse-il-1-elisa-kit","title":"Mouse IL-1β ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e\n1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\n2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\n3. Distilled water or deionized water\u003cbr\u003e\n4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\n5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\n\n\u003cstrong\u003eOne, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\n1. Sample collection and storage\u003cbr\u003e\n① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\n2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e)\u003cbr\u003e\n1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\n②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\n③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\n④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\n⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\n⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 1000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (1000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\n\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/9297927b5c1b4d6dbdde6fd6fcaad53d.png\" alt=\"\" width=\"433\" height=\"245\"\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n1. Ready for all the needed reagent and standard;\u003cbr\u003e\n2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\n3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\n4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\n5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\n6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\n7. Repeat the plate washing operation of step 5;\u003cbr\u003e\n8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\n9. Repeat step 5 to wash the plate;\u003cbr\u003e\n10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\n11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\n12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\n13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240104\/6748f65fcdda465ba54e1c682648f699.jpg\" alt=\"\" width=\"312\" height=\"242\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n1. Recovery: Different levels of Mouse IL-1\u0026amp;beta were incorporated into the cell culture medium samples; And measure its recovery. The recovery ranges from 69 to 102%, with an average recovery of 77%.\u003cbr\u003e\n2. Sensitivity: Mouse IL-1β The minimum detectable dose (MDD) is generally less than 3.8 pg\/mL. The lowest detectable dose was calculated as the corresponding concentration based on the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\n3. Calibration: The ELISA kit was purified recombinant Mouse IL-1\u0026amp;beta expressed in E. coli. Corrected by protein.\u003cbr\u003e\n4. Linearity: four different samples were mixed with high concentrations of Mouse IL-1β , and then with diluent (1×) Measure linearity by dilating the sample to within the detection range.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e103\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e101-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e103-110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e105-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n5. Specificity: The ELISA could detect native and recombinant Mouse IL-1\u0026amp;beta. Protein. Combine the following factors with diluent (1×) Configure to a concentration of 50ng\/mL to detect with Mouse IL-1β Cross reaction with mouse IL-1 \u0026amp; beta. 50ng\/mL of interfering factor was incorporated into the intermediate range of recombinant Mouse IL-1β To detect the effect on Mouse IL-1β The interference. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; text-align: center; height: 22px;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1Ra\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIL-1RI\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003eIL-1RII\/Fc Chimera\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height极客 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too many, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\n\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 极客 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.8pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-1β antibody was precoated on a high-affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-1β present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-1β Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-1β standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-1β detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe interleukin-1 (IL-1) protein family contains the classical members IL-1α, Il-1β and IL-1ra, as well as IL-18, IL-33 and IL-1F5-10. IL-1α and IL-1β bind to the same cell surface receptor and share biological functions. \"With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, unstimulated cells from healthy mice do not produce IL-1.\" \"However, in response to inflammatory agents, infections, or microbial endotoxins, IL-1 is produced in large quantities by macrophages and a variety of other cells.\" IL-1β plays a key role in the physiological processes of immune response and inflammation, bone remodeling, fever, glucose metabolism, growth hormone \/IGF-1. \"Abnormal or sustained production of IL-1 has been associated with a range of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and aging-related diseases.\" \u003cbr\u003eIL-1α and IL-1β have structurally related peptides that share approximately 25% identity at the amino acid level. They were synthesized as the size of 31 kDa precursor, and then cracking into mature protein, molecular weight is about 17.5 kDa. Caspase 1 \/ ICEI cracking IL - 1 beta precursor is a key step in the inflammatory response. Neither IL-1α nor IL-1β contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through nonclassical pathways. Part of raw IL - 1 alpha can appear on the cell membrane, and may have biological activities. Unlike precursor IL - 1 alpha, beta IL - 1 precursor compared with mature body almost do not have biological activities. Both unprocessed and mature IL-1β can be exported from the cell. \u003cbr\u003eIL - 1 alpha and beta IL - 1 with IL - 1 ra combination immunoglobulin superfamily receptors play a role. \"Cells expressing the 80-kda transmembrane type I receptor (IL-1RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid lining cells, chondrocytes, and hepatocytes.\" \"Cells expressing the 68kDa transmembrane type II receptor (IL-1RII) include B cells, neutrophils, and bone marrow cells.\" \"The two IL-1 receptors share approximately 28% identity in their extracellular domains, but a significant difference is found in their intracellular domains: the type II receptor has an intracellular domain of only 29 amino acids, whereas the type I receptor has an intracellular domain of 217 amino acids.\" IL-1RII does not produce a signal for IL-1 and may act as a decoy receptor to attenuate IL-1 function. IL-1 receptor accessory protein (IL-1RAcP) is associated with IL-1RI and required for IL-1RI signal transduction. IL-1ra is a secretory molecule that acts as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII can be detected in human plasma, joint synovial fluid, and conditioned media of a variety of human cell lines. In addition, IL-1binding proteins similar to soluble IL-1RII are capable of being encoded by cowpox and vaccinia virus.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1\u0026amp;times) is used. \"In addition to dilution, cell culture medium may be used for other intermediate dilutions.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518150975563,"sku":"abs520001-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7ac31d984f114771b7dbeb565f671ef6.jpg?v=1792753492"},{"product_id":"mouse-il-elisa-kit","title":"Mouse IL-10 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e18.5pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse il-10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-10present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse il-10detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.2 - 2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e95-103%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMouse interleukin -10 (IL-10) also known as cytokine synthesis inhibitor (CSIF). It is IL-10 \u0026amp; Alpha-a member of the helical cytokine family, which also includes il-19, il-12, il-22, IL-24 , and il-26\/ak155. Many activated hematopoietic stem cells, hepatic stellate cells, keratinocytes, and placental cytotrophoblasts secrete il-10. Human IL-10 is active in mouse cells, but mouse IL-10 does not act on human cells. The amino acid homology of mature mouse IL-10 and rat IL-10 is 85%, and that of cattle, dogs, horses, cats, humans, sheep, and pigs is 70~77%. Mature mouse IL-10 is a noncovalently bound homodimer with a molecular weight of 36 kDa containing two intrachain disulfide bonds.\u003c\/p\u003e\n\u003cp\u003eIL-10 by the II type cytokine receptor IL-10 r\u0026amp; Alphaand IL-10 \u0026amp; amp; Betathe heteromeric receptor complex that is composed of exerts its biological activityIL-10 r\u0026amp; Alphais a 110 kDa transmembrane glycoprotein expressed in lymphocytes, natural killer cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while 75 kDa il-10r\u0026amp; Beta\u003c\/p\u003e\n\u003cp\u003eIL-10 is involved in immune regulation of inhibitory and activating effectsIL-10 as an anti-inflammatory cytokine, promotes the formation of M2 macrophages by inhibiting the activation and expansion of Th1 cells and Th17 cells. Immunosuppression regulates the expression of T cells and B cells, which play an important role in Treg cell proliferation (19) . In the tumor microenvironment, IL-10 inhibition regulates the proliferation of T cells and myeloid derived suppressor cellsIL-10 induces the accumulation of intratumoral material and activates cd8+ T cellsIL-10 plays an important protective role in limiting tissue damage in joint inflammation and promoting muscle regrowth after injury, but also contributes to persistent viral infection. IL-10 is elevated in Sjogren's syndrome (saliva) and primary central nervous system lymphoma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152220747,"sku":"abs520005-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/773aa4393f904fd0b8ef1159a663e229.jpg?v=1792753513"},{"product_id":"mouse-il-p70-elisa-kit","title":"Mouse IL-12 p70 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 2500pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (2500pg\/mL), dilute with buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cbr\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/9fc40f0430674440a0dca6993aaabcf5.png\" alt=\"\" width=\"555\" height=\"286\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/31104240a4094db3bbb14da2f3b0bdec.jpg\" alt=\"\" width=\"249\" height=\"194\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided by \u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery: Different levels of mouse IL-12 p70 were incorporated into the cell culture medium samples, and the recovery rate was determined. Recoveries ranged from 83 to 107%, with an average recovery of 93%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of murine IL-12 p70 was generally less than 2.24pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 12 p70 protein in mice. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse IL-12 p70, followed by dilution (1×). Linearity was determined by dilating the samples to within the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 80.7041%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e99-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e98-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e104\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e95-112\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e93-107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e5. Specificity: the ELISA method to detect natural and restructuring IL - 12 p70 protein in mice. The following factors in diluent mixture 50 ng\/mL (1 x) to detect the concentration of IL - 12 p70 cross reaction with mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 12 p70 reference substance in the restructuring of the mice, to detect the interference of IL - 12 p70 in mice. No significant cross-reactivity or interference was observed. The recombinant rat IL-12 p70 containing 1.25ng\/mL had cross reactivity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 132px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p70\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p35\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p40 monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-12p40 monomer\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL - 12 p40 homologous dimers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit with double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti mouse antibody of IL - 12 p70 pre package is on the high affinity enzyme label plate. After incubation, the IL-12 p70 in the sample was combined with the solid phase antibody and the detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-12 p70 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-12 p70 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-12 p70 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-12 (IL-12, also known as NKSF), a 70-75 kda heterodimer glycoprotein, belongs to the IL-12 heterodimer cytokine family. IL - 12 by 35 kda (p35) and 40 kda (p40) of two subunits, the two subunits by disulfide bond is linked together, no amino acid sequence homology between each other. The mature p35 subunit is 196 amino acids long, containing seven cysteines and a potential N-linked glycosylation site. Mature mouse p35 shares 63% and 86% amino acid identity with human and rat, respectively. Mature mice p40 subunits long 313 amino acids, including 13 cysteine and five potential glycosylation sites of N - connection. Some studies have reported that the p40 sequence is polymorphic, but this IL-12 p70 kit does not recognize these allelic products. \u003cbr\u003emature mouse p40 has 72% and 93% amino acid identity with human and rat, respectively. Although p35 resembles a ligand for erythropoietin, p40 is more similar to the N-terminus of the erythropoietin receptor, with WSXWS domains, an immunoglobulin-like domain, and four conserved cysteines. This suggests that IL-12 may be a cytokine receptor mimetic, similar to the IL-6\/ soluble IL-6R complex. It is worth noting that the p40 can exist in the form of monomer or homologous dimers, but never found p35 monomer form. p40 is the larger of the two subunits of interleukin 23 (IL-23). Although IL-12 has long been considered a secretory molecule, membrane-bound IL-12 has also been found in human and mouse cells. Il-12-producing cells include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8+DC (mouse cells only), and non-germinal center (CD38-CD44+) B cells (human cells only). The high-affinity receptor for \u003cbr\u003emouse IL-12 is composed of at least two type I transmembrane glycoproteins, similar to members of the cytokine receptor superfamily. Beta 1 first subunits (R) of 100 kda, with Kd = 1 nm and IL - 12. This receptor is the primary binding site for the p40 subunit; The second subunit (Rβ2) is 130kDa and has no amino acid homology with the Rβ1 subunit. The receptor is an important part of signal transduction, as a disulfide bond connected p30 - p40 dimers attachment points. As mentioned above, mouse p40 exists as a monomeric, dimeric form, and binds either at p35 to form IL-12 or at p19 to form IL-23. Both homodimer p40 and IL-23 can bind IL-12R and act as antagonists that do not transmit signals. Alternatively, p40 homodimer can also bind to Rβ1 and activate microglia and macrophages. Functionally, IL-12 has been shown to enhance cytotoxicity and induce interferon-γ production by NK cells, T cells, and dendritic epidermal T cells. It has been reported that IL-12 can induce the production of interferon-γ by macrophages. Together with its IL-12 family members such as IL-23 and IL-27, IL-12 can promote the progression of CD4+ Th1-type immune responses. In response to infection, IL-27 is secreted first to promote the excess of TH0 to THR0\/1, followed by IL-12 secretion to generate Th1 effector cells. \"Production together with IL-18 and IL-12 transforms effector cells into Th1 memory cells, which are then activated by IL-23.\"\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eUnopened kits were stored at 2-8 ° C. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e39.06pg\/mL-2500pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152253515,"sku":"abs520006-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/194789392b3e421988c988138a86cbf3.jpg?v=1792753510"},{"product_id":"mouse-ifn-elisa-kit","title":"Mouse IFN-γ ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the samples immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 2000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (2000pg\/mL), and the dilution buffer (1×) Can be used as a standard curve zero (0 pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/3ca1b770c3c2458896bdd173379097de.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards; \u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution. \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/ba161fb276f04f058cee473d92d99563.jpg\" alt=\"\" width=\"310\" height=\"241\"\u003e\u003cstrong\u003e\u003cstrong\u003eNote: The standard curve data provided is for reference only. The sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e1. Recovery: Different levels of mouse IFN-\u0026amp;gamma were incorporated into the cell culture medium samples; And the recovery rate was determined. The recovery ranges from 82 to 110%, with an average recovery of 94%. \u003cbr\u003e\u003cbr\u003e2. Sensitivity: mouse IFN-γ The minimum detectable dose (MDD) of murine IFN - \u0026amp; gamma was generally less than 1.8pg\/mL. The lowest detectable value is the corresponding concentration calculated from the mean of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was prepared with highly purified recombinant mouse IFN-\u0026amp;gamma expressed in E. coli. Corrected by protein. \u003cbr\u003e\u003cbr\u003e4. Linearity: four different samples were mixed with high concentrations of mouse IFN-γ , and then with diluent (1×) Measure linearity by dilating the sample to within the detection range. \u003cbr\u003e\u003cbr\u003e5. Specificity: The ELISA could detect both native and recombinant mouse IFN-γ Protein. Combine the following factors with diluent (1×) Formulated to a concentration of 50ng\/mL to detect the difference with mouse IFN-γ The cross reaction. 50ng\/mL interfering factor was incorporated into the intermediate range of recombinant mouse IFN-γ To detect the effect of IFN-\u0026amp;gamma on mice; The interference of IFN - \u0026amp; gamma. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e4, common problem resolution\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit with double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse IFN-γ antibody was precoated on a high-affinity microplate. Enzyme label plate hole with standard substance, the sample under test and biotinylated antibodies detection, after incubation, samples of IFN - gamma combined with solid phase antibody and detection antibody, immune complex. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, adding chromogenic substrate, dark color. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IFN-γ Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IFN-γ standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IFN-γ detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterferon-γ (IFN-γ, also known as type II interferon) is an important immunomodulatory cytokine, which has been found to have antiviral activity. IFN-γ plays a key role in the host defense process through its antiviral, antiproliferative and immunomodulatory functions. \"In many cell types, IFN-γ induces cytokine production and upregulates the expression of a variety of membrane proteins, including types I and II major histocompatibility complex (MHC) antigens, Fc receptors, leukocyte adhesion molecules, and B7 family antigens.\" IFN-γ is a potent activator of macrophage response. It directs the synthesis, conversion, and secretion of immunoglobulin by B cells. IFN-γ affects the development of T helper cell phenotype by inhibiting the differentiation of Th2 cells and stimulating the development of Th1 cells. IFN-γ plays an important role in the progression of autoimmune diseases and inflammatory diseases such as atherosclerosis. \u003cbr\u003ebioactive IFN - gamma is made up of two non covalently linked with different degree of glycosylation of 20 to 25 kda homologous dimer composed of subunits. Mature mouse IFN-γ shared 86% amino acid sequence identity with rat IFN-γ, and 38-44% amino acid sequence identity with bovine, dog, cotton rat, horse, cat, human, pig and rhesus monkey IFN-γ. The IFN-γ dimers assemble before the transmembrane IFN-γRI(α subunit) and then bind to the transmembrane IFN-γRII(β subunit), thus forming an active receptor complex containing two α and β subunits. IFN-γRII in the receptor complex increases ligand affinity as well as the efficiency of signal transduction. Although the α chain is widely expressed in a variety of cell types, the receptor β chain is associated with the state of IFN-γ response and its expression is tightly regulated. \u003cbr\u003eUnder inflammatory conditions, IFN-γ can be produced by a variety of cell types, including dendritic epidermal \/γδT cells, keratinocytes, peripheral γδT cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, natural killer cells, CD4+T cells, and testicular spermatids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152351819,"sku":"abs520007-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6efb12a8026c42c0a9719bf933732ba2.jpg?v=1792753515"},{"product_id":"mouse-tnf-elisa-kit","title":"Mouse TNF-α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1.  Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2.  High precision liquid dispenser and disposable tip \u003cbr\u003e3.  Distilled or deionized water \u003cbr\u003e4.  Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e1.  Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e2.  Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand  \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 2000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 2000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/3ca1b770c3c2458896bdd173379097de.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e1.  Prepare all required reagents and standards; \u003cbr\u003e2.  Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3.  Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4.  Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5.  The liquid in the plate is sucked off and the plate is washed using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the intra-plate wash liquid is aspirated. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6.  Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7.  Repeat th 5 Step washing operation; \u003cbr\u003e8.  Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9.  Repeat th 5 Step washing operation; \u003cbr\u003e10.  Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11.  Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12.  After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13.  Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/c1c8507ef90b434c97bb474ed5498e92.jpg\" alt=\"\" width=\"381\" height=\"296\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e1.  Recovery: Different levels of mice were spiked in cell culture medium samples TNF-α The recovery rate was determined. The recoveries range from 88-107% , the average recovery was in 94% 。 \u003cbr\u003e2.  Sensitivity: Mouse TNF-α The lowest measurable dose ( MDD ) is generally less than 5.5pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e3.  Correction: This ELISA High purity recombinant mice expressed by Escherichia coli TNF-α Corrected by protein. \u003cbr\u003e4.  Linearity: 4 High concentrations of mice were spiked into different samples TNF-α , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003e Dilution factor \u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003e Average \/ Expected value ( % ） \u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003e Range ( % ） \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e97\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e94-103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e99-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e97-114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 14px;\"\u003e104-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5.  Specificity: This ELISA Method can detect natural and recombinant mice TNF-α Egg whites. The following factors were mixed with diluent ( 1× ) formulated into  50ng\/mL Concentration to detect with mice TNF-α Cross-reactivity of. Will 50ng\/mL Interfering factors incorporated into the intermediate range of recombinant mice TNF-α In the control article, to detect the effect on mice TNF-α Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.3268%; height: 120px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003e Recombinant human protein \u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; text-align: center; height: 22px;\"\u003e Recombinant mouse protein \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eLymphotoxin α1\/β2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF RII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eLymphotoxin α2\/β1\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eTNF RI\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eTNF RI\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e1.  Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Improper storage of kits; Mixing reagents of different kits \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Purchase new kits and pay attention to storage conditions; Not mixable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Low incubation temperature and short time \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e If the temperature is low, extend the incubation time and color development time \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Wrong addition or missed addition of reagents \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Add the correct reagent strictly according to the instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e The container used to prepare the solution is not clean or there is a problem with the water \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Use clean containers and qualified distilled water \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e In the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Follow the instructions strictly \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Heterogeneity of reagent temperature \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e All reagents should be equilibrated at room temperature 30 minute \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003e Detecting antibodies and \/ Or HRP Concentration too low \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003e Refer to the instructions, do not dilute at will \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2.  Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Less washing times, insufficient \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Wash as per instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Substrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Use clean containers and qualified distilled water during preparation; Store protected from light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Incubation temperature is high and \/ Or too long \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Controlling the temperature and time of incubation and final enzymatic reaction \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e The gun tip was not changed during sample loading, resulting in cross-contamination \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Change the gun head for each sample \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Cross contamination of nearby holes \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Vertical clapper, use suitable clapper paper to avoid paper scraps in the hole \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Presence of endogenous interfering substances in sample \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Estimate possible infectious substances and perform corresponding treatment \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003e Samples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003e Avoid hemolysis, contamination, long storage and other phenomena \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 5. Experimental flow chart \u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.0pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-mouse TNF-α antibodies were pre-coated on high affinity enzyme labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAPC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNFA, TNF-A, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha, Mouse tumor necrosis factor alpha ELisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003e form \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003e Specifications \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003e Shelf life of diluted or redissolved reagent after unpacking \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse TNF-α Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 Block \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003e After the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse TNF-α Standard \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003e After dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse TNF-α Detection antibodies \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e After the concentrated volume is dissolved, it can be in 2-8°C store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 Vials \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10× Buffer for concentration and dilution \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003e After opening, it can be found in 2-8°C store 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003e Chromogenic liquid \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003e Stop Solution \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20× Concentrated Wash Buffer \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e Sealing film \u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 Sheets \u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003e Stored at room temperature, to avoid contamination, not reusable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototype ligand of tumor necrosis factor superfamily. It is a pleiotropic factor that plays a central role in inflammatory response, immune system development, apoptosis and lipid metabolism. TNF-α is also involved in many pathological processes, including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type II diabetes, septic shock, autoimmunity, and cancer. Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE\/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa.\u003cbr\u003eTNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518152974411,"sku":"abs520010-96T","price":317.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2daa7c3a973247cab17a0308debd0bd8.jpg?v=1792753523"},{"product_id":"il-13-elisa-kit","title":"Mouse IL-13 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength) \u003cbr\u003e2. High precision liquid dispenser and disposable suction head \u003cbr\u003e3. Distilled water or deionized water \u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute. \u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards \u003c\/strong\u003e\u003cstrong\u003edo double hole detection \u003c\/strong\u003e) \u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample: \u003c\/strong\u003eTake 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up. \u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample: \u003c\/strong\u003eTake 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made. \u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample: \u003c\/strong\u003e10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody. \u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample: \u003c\/strong\u003eused 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody. \u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day. \u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"434\" height=\"244\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Ready for all the needed reagent and standard; \u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it; \u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry; \u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours. \u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper; \u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature; \u003cbr\u003e7. Repeat the plate washing operation of step 5; \u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light; \u003cbr\u003e9. Repeat step 5 to wash the plate; \u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light; \u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly; \u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240313\/424653e603b94cd38f1c549a9c8f408c.jpg\" alt=\"\" width=\"278\" height=\"216\"\u003eNote: The standard curve data provided is for reference only. The sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery: Different levels of mouse IL-13 were incorporated into the cell culture medium samples, and the recovery rate was determined. The recovery ranged from 96 to 103%, with an average recovery of 99%. \u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of IL-13 in mice was generally less than 0.089-0.740pg\/mL. The lowest detectable value of IL-13 was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations. \u003cbr\u003e3. Correction: the ELISA kit by high purity recombinant e. coli expression correction by IL - 13 proteins in mice. \u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse IL-13, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e100-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e102-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e103-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e100-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse IL-13 protein. The following factors in diluent (1 x) configured to the concentration of 50 ng\/mL to detect cross reaction with IL - 13 in mice. To the interference of 50 ng\/mL factor added to the middle scope IL - 13 reference substance in the restructuring of the mice, the interference of IL - 13 to test in mice. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 108px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\" colspan=\"2\"\u003eRecombinant human proteins\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 20px; text-align: center;\" colspan=\"2\"\u003eRecombinant mouse proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eG-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIL-10R\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eLIF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIFN-γ\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eIL-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; height: 22px;\"\u003eMIP-1α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eTNF-α\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eMIP-1β\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%;\"\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eUsed to configure the solution container not clean, or there is something wrong with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of washing the plate, the soaking time is long, the number of washing the plate is too much, and the impact of washing the plate is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual operation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe temperature of the reagent was not uniform\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents were equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection of antibody and\/or HRP concentrations was too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eDo not dilute at will according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e2. Flower plate (blank, negative and positive controls were normal, but the OD value of sample Wells was significantly higher)\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eFewer washing, inadequate\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eWash according to instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe substrate 3,3',5,5' -tetramethylbenzidine (TMB) is contaminated or exposed to metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eThe use of clean containers and when making up qualified distilled water; Avoid light preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive incubation time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eControl incubation and the enzymatic reaction temperature and time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSample did not change when the spear head, cause cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eChange the tip of each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eNear the hole cross contamination\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eVertical clappers, using the appropriate legal pad, avoiding the hole into confetti\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eSamples are endogenous interfering substance\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePossible infectious agents were speculated and treated accordingly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eSample hemolysis, storage for too long, incomplete agglutination, contaminated by bacteria, blood vessels to add impact\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eAvoid hemolysis, contamination, too long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.65pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDouble antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-13 antibody was precoated on a high-affinity microplate. After incubation, the IL-13 present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove not combined with material, by adding horseradish peroxidase labeled chain mildew avidin (Streptavidin - HRP). After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse IL-13 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse IL-13 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse IL-13 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-13 (IL-13), a member of the chemokine family, plays a key role in the pathogenesis of allergy, cancer and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils and visceral smooth muscle cells. IL-13 inhibits the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE and upregulation of CD23\/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha1 and IL-4 R alpha. The compound is used as a type 2 receptor IL - 4. IL-13 ralpha2 binds IL-13 and prevents IL-13 from signaling through the IL-13R alpha1\/IL-4 Ralpha complex. Il-13-bound IL-13 R alpha2 can directly promote tumor cell invasion and tissue fibrosis development.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153203787,"sku":"abs520012-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/71872e1ce512478d800d1f2b4fde2c06.jpg?v=1792756817"},{"product_id":"mouse-cxcl10-elisa-kit","title":"Mouse CXCL10\/IP-10\/CRG-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and 540nm or 570nm correction wavelength)\u003cbr\u003e\u003cbr\u003e2. High precision liquid dispenser and disposable suction head\u003cbr\u003e\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e\u003cbr\u003e4. Washing bottle (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\u003cbr\u003e5. 500mL cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOne, preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particles should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended that the sample be divided according to the dosage and stored in the refrigerator at -20 ° C to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e② Serum: Samples were collected using a serum separation tube (SST) and samples were left at room temperature for 30 minutes. The samples were centrifuged at 1000g for 15 min. Serum was immediately removed and tested immediately. If the sample is not tested in time after collection, it is recommended to repack according to a single dosage and freeze in ≤ -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin, or citric acid as an anticoagulant, centrifuged at 1000g for 15 min within 30 min of collection, and tested immediately. If the samples are not detected in time after collection, it is recommended to separate the samples according to the single dosage and freeze them in \u0026amp;le. -20℃ refrigerator to avoid repeated freezing and thawing. Samples may need to be diluted (1×) Dilute.\u003cbr\u003e\u003cbr\u003e2 Reagent preparation (\u003cstrong\u003ePlace all reagents and samples at room temperature for\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eminutes before use. It is recommended that all experimental samples and standards\u003c\/strong\u003e\u003cstrong\u003edo double hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e1× Preparation of washing solution: concentrated washing solution in the kit is 20× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working liquid. \u003cstrong\u003eExample:\u003c\/strong\u003e Take 10mL concentrated washing solution +190mL distilled water to 200mL, the actual operation can be calculated first, then make up.\u003cbr\u003e\u003cbr\u003e②1× Dilution with buffer preparation: concentrate dilution in the kit with buffer 10× Mother liquor, diluted to 1\u0026amp;times with distilled water before use; Working solution. \u003cstrong\u003eexample:\u003c\/strong\u003e Take 3mL of concentrated dilution with buffer +27mL of distilled water to a constant volume of 30mL. In practical operation, the required dilution buffer can be calculated according to the dilution multiple of the sample, and then the preparation can be made.\u003cbr\u003e\u003cbr\u003e③ Detection of antibody: the dry powder was centrifuged to the bottom of the tube, and 110uL dilution buffer (1×) was used. Dissolve and let stand at room temperature for 5 minutes to obtain 100× Mother liquor; Dilute to 1\u0026amp;times before use; Working solution. Calculate the desired volume by using 100uL per well. \u003cstrong\u003eexample:\u003c\/strong\u003e 10 Wells were used, then take 10uL of 100 times the working concentration of the test antibody, using dilution buffer (1×) Constant volume to 1mL, get 1mL of 1× The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e④SA-HRP: SA-HRP is 40× Mother liquor, use dilution buffer before use (1×) Dilute to make 1× Working solution, 100uL required per well. \u003cstrong\u003eexample:\u003c\/strong\u003e used 10 holes, then take 25uL of 40× Mother liquor +975uL dilution buffer (1×) Constant volume to 1mL to obtain 1\u0026amp;times of 1mL; The working concentration of the detected antibody.\u003cbr\u003e\u003cbr\u003e⑤ Chromogenic agent: according to 100uL per well, calculate the amount needed for this test, take out the corresponding volume of chromogenic agent, avoid light; The removed chromogenic agent is only used on the same day.\u003cbr\u003e\u003cbr\u003e⑥ Standards: lyophilized standards with dilution buffer (1×) Redissolve, redissolve volume 1000uL, to obtain a concentration of 4000pg\/mL standard mother liquor. Gently shake for at least 5 minutes, it is fully dissolved. Add 300uL dilution buffer (1×) to each dilution tube. . The standard mother liquor is diluted according to the picture below, and each tube must be fully mixed before pipetting to the next tube. The standard mother liquor without dilution can be used as the highest point of the standard curve (4000pg\/mL), and the dilution buffer (1×) Can be used as the zero point of the standard curve (0pg\/mL).\u003cdiv\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/5a634dc406d84945801a7c8408680152.png\" alt=\"\" width=\"434\" height=\"244\"\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e2, operation steps \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2 Remove the microplate from the sealed bag that has been balanced to room temperature, and put the unused slat back into the aluminum foil bag and re-seal it;\u003cbr\u003e\u003cbr\u003e3. Add 300uL washing solution to the microplate, let it soak for 30 seconds, discard the washing solution and pat the microplate dry on absorbent paper, please use immediately do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentrations of standard, experimental samples or quality control into the corresponding Wells, 100uL for each well. The Wells were sealed with plate adhesive and incubated at room temperature for 2 hours.\u003cbr\u003e\u003cbr\u003e5 Suck the liquid out of the plate and wash the plate using a bottle washer, a multi-channel plate washer, or an automatic plate washer. Add 300uL of washing liquid to each well, and then suck the washing liquid out of the plate. Repeat 3 times. Every time you wash the plate, try to absorb the residual liquid to help you get a good test result. At the end of the last plate wash, please blot all the liquid in the plate or invert the plate and pat all the residual liquid in the absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100uL detection antibody to each microwell. Seal the reaction Wells with sealer tape and incubate for 2 hours at room temperature;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation of step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100 ULSA-HRP to each microwell and incubate for 20 minutes at room temperature. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat step 5 to wash the plate;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of color development solution to each microwell, incubate at room temperature for 5-30 minutes, pay attention to avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of termination solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution turns green or the color change is inconsistent, tap the microplate to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after the termination solution is added, the absorbance value at 450nm is measured using a microplate reader and 540nm or 570nm is set as the correction wavelength. If the dual wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13 Calculation results: The corrected absorbance values (OD450-OD540\/OD570), the compound reading were averaged for each standard and sample, and then the average zero standard OD value was subtracted. Standard curves were created by 4-parameter logic (4-PL) curve fitting using computer software. Alternatively, a curve can be generated by plotting the logarithm of the concentration of the standard against the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process produces an adequate but less accurate fit to the data. If the sample is diluted, the concentration should be multiplied by the dilution.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240116\/b1974362223e44e183d920c17ee108df.jpg\" alt=\"\" width=\"302\" height=\"235\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote: The standard curve data provided in\u003c\/strong\u003eare for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e1. Recovery: Different levels of mouse CXCL10\/IP-10\/CRG-2 were incorporated into the cell culture medium samples to determine the recovery rate. The recovery ranged from 90 to 116%, with an average recovery of 103%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The minimum detectable dose (MDD) of murine CXCL10\/IP-10\/CRG-2 was generally less than 7.8pg\/mL. The lowest detectable value was calculated as the corresponding concentration based on the average of the zero absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Calibration: The ELISA kit was calibrated with recombinant mouse CXCL10\/IP-10\/CRG-2 protein expressed in E. coli.\u003cbr\u003e\u003cbr\u003e4. Linearity: Four different samples were mixed with high concentrations of mouse CXCL10\/IP-10\/CRG-2, followed by dilution (1×). Linearity was determined by dilutes the samples to the detection range.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 102px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution ratio\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eMean value\/expected value (%)\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e96-109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e107-115\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 23px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 23px;\"\u003e111\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 23px;\"\u003e104-121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 10px;\"\u003e106\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 10px;\"\u003e91-114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e5. Specificity: This ELISA can detect native and recombinant mouse CXCL10\/IP-10\/CRG-2 proteins. The following factors were diluted (1×) A concentration of 50ng\/mL was formulated to detect cross-reactivity with mouse CXCL10\/IP-10\/CRG-2. To the interference of 50 ng\/mL factor added to the middle range of restructuring the mice CXCL10 \/ IP - 10 \/ CRG - 2 reference substance, to test in mice CXCL10 \/ IP - 10 \/ CRG - 2. No significant cross-reaction or interference was observed.\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 78.4876%; height: 108px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 20px; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 20px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eI-TAC\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eIP-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eLIX\/GCP-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003eMIG\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003ePF-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e\u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e4, common problem resolution\u003cbr\u003e1. The white board (no color), after the completion of color\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eNO.\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003eCause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eKit stored improperly; A mixture of different kit reagent\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eBuy a new kit, pay attention to the storage conditions; Do not mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eEndow low temperature, short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is too low, the incubation time is prolonged and the color development time is prolonged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or omission of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIn strict accordance with the manual steps to add the correct reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height极速赛车开奖直播历史记录\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Theory \u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse CXCL10\/IP-10\/CRG-2 antibodies were precoated on high-affinity microplate. After incubation, CXCL10\/IP-10\/CRG-2 present in the sample combined with the solid-phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values.\u0026lt;\/td\u0026gt;\u0026lt;\/tr\u0026gt;\u0026lt;tr\u0026gt;\u0026lt;td\u0026gt;\u0026lt;strong\u0026gt;Composition\u0026lt;\/strong\u0026gt;\u0026lt;\/td\u0026gt;\n\u0026lt;td\u0026gt;\u0026lt;table style=\" border-collapse: collapse width: margin: auto page-break-inside: avoid height: border=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eOnce opened, diluted or heavy soluble reagent expiration date\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 standard substance\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eDissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eMouse CXCL10\/IP-10\/CRG-2 detect antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eEnrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 x condense dilute with buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, can be in 2-8 ° C stored for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eColor-substrate solution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 x inspissation washing buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eRoom temperature storage, to avoid contamination, do not repeat use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChemokine (C - X - C motif) ligand 10 (CXCL10) belongs to CXC chemokines family a small cytokines, also known as 10 - kda interferon gamma induced proteins (beta IP10 or IP - 10). In response to IFN-α, CXCL10 is secreted by several cell types. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 is thought to have a variety of effects, such as chemoattractant to monocytes and T cells, promotion of T cell adhesion to endothelial cells, anti-tumor activity and inhibitory effects on bone marrow colony formation and angiogenesis. IP-10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. CRG-2, the mouse homolog of human IP-10, shares approximately 67% amino acid sequence identity with human IP-10.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period. \u003cbr\u003e2. Components of different kits and different batch kits should not be mixed. \u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. \"If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used.\" \u003cbr\u003e4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. \u003cbr\u003e5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. \u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, 2-8° C Storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e62.5pg\/mL-4000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153236555,"sku":"abs520013-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8ce06aa8ec8147989f36bf994ebc6a2d.jpg?v=1792756819"},{"product_id":"ccl5-elisa-kit","title":"Mouse CCL5\/RANTES ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.2pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse ccl5\/rantes antibody was pre coated on a high affinity microplate. Standards, samples to be tested and biotinylated detection antibodies are added into the wells of enzyme plate. After incubation, mouse ccl5\/rantes present in the samples are combined with solid-phase antibodies and detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse ccl5\/rantesdetection antibody, dilution buffer, chromogenic solution (a, b), washing liquid, stop liquidSa-hrp, sealing film and instruction manual.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.3-2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003cstrong\u003e96-103%\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520014.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520014.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eChemotactic cytokineChemotaxis to leukocytes is an important initial step in the process of inflammation, and it is also an important aspect of the body's innate immune function in defense and clearance of foreign bodies such as invading pathogens. RANTES, which regulates the expression and secretion of normal T cells, is a member of the CC subfamily of chemotactic cytokines, also known as CCL5. The protein has a wide range of sources, most of which are expressed in the inflammatory factor IL-1 \u0026amp; amp; Beta; Or tnf-\u0026amp; Alpha; CCL5 can be released under the stimulation of. Generally, CCL5 is mainly secreted by cd8+t cells, epithelial cells, fibroblasts and platelets.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eCCL5, like other chemotactic cytokines, can bind to its specific receptors in monomeric or multimeric form. CCL5 has a dimeric structural basis, forming multimers at high concentrations, and often exists as monomers after endocytosis. Glycosaminoglycans (GAGs) on the cell surface can lead to the multimerization of CCL5. Multimerized CCL5 can not only activate inflammatory cells, prolong and aggravate inflammatory responses, but also regulate cell growth and differentiation. When CCL5 is monomeric or dimeric or its concentration is nanomolar, its receptors are CCR1, CCR3, CCR4 and CCR5, which have chemotactic activity, can enhance cytotoxicity and inhibit HIV replication. The multimeric form or micromolar level of CCL5 mainly leads to the activation of T cells, monocytes and macrophages by binding with gags on the cell surface, and enhances HIV replication.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eCCL5 has chemotactic effects on a variety of leukocytes: 1) monocytes; 2) Chemotaxis of unstimulated cd4+cd45ro+ memory T cells and activated cd4+ and cd4+t cells; 3) Chemotaxis eosinophils and stimulates their degranulation and respiratory burst; 4) It is a strong chemotactic agent for basophils, but its effect on stimulating histamine release is weak; 5) Pretreatment of T cells can increase their ability to adhere to endothelial cells stimulated by IL-1. These properties make CCL5 play an important role in immune regulatory function and autoimmune disorders and inflammatory processes.\u003c\/p\u003e\n\u003cp\u003eAs a chemokine, CCL5 not only participates in inflammatory response and mediates leukocyte migration and infiltration, but also regulates cell growth and differentiation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153465931,"sku":"abs520014-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3f05d7bbeea24687af9d513ce3983998.jpg?v=1792756822"},{"product_id":"gm-csf-elisa-kit","title":"Mouse GM-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.75pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti mouse GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, Serum\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspgm-csfdetection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003eto be improved\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81-500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e84-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreservation Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520015.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520015.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGM-CSF (granulocyte macrophage colony stimulating factor)Induce the development of monocytes, neutrophils, eosinophils, myeloid dendritic cells and dermal dendritic cells. It also acts as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. GM-CSF signaling is mediated through the receptor complex, which is composed of GM-CSF R \u0026amp; amp; Alpha; And beta C subunit, syndecan-2 as a potential co receptor. The beta C subunit is shared by the receptor complex of IL-3 and IL-5.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153498699,"sku":"abs520015-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2365d8c6008445ea9dec2abffd0e6a52.jpg?v=1792756825"},{"product_id":"ccl2-elisa-kit","title":"Mouse CCL2\/JE\/MCP-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.9pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti mouse ccl2\/je\/mcp-1 monoclonal antibody is coated on the microplate. Ccl2\/je\/mcp-1 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse ccl2\/je\/mcp-1 polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eTest Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eTest Sample Type: \u0026lt;\u003c\/strong\u003ecell supernatant, Serum, plasma\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSample loading: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003ePrecoated 96well plates, standards, anti mouse  ccl2\/je\/mcp-1one copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003eSee the instructions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e3.91-250 pg\/ml\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e84-110%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eSaving Method: \u003c\/strong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e2-8\u003c\/span\u003e℃\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cstrong\u003e\u003cspan lang=\"EN-US\" style=\"font size: 10pt; font family: Microsoft YaHei, sans serif;\"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520016.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003cbr\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCCL2\/je\/mcp-1 is a chemokine that binds to the receptor CCR2 and induces monocyte chemotaxis. It induces the activation of monocytes, NK cells, lymphocytes and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to the site of inflammation contributes to atherosclerosis, polydactylySclerosis and the severity of allergic asthma disease. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518153531467,"sku":"abs520016-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/29bdc8f2b3e7439086b968e7807a5eec.jpg?v=1792756826"},{"product_id":"il-8-elisa-kit","title":"Mouse CXCL1\/KC (IL-8) ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.09pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti mouse cxcl1\/kc monoclonal antibody is coated on the microplate. Cxcl1\/kc in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse cxcl1\/kc polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspcxcl1\/kca copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003esee the instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e84-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520017.jpg\" width=\"\" alt=\"\" height=\" 190 \"\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520017.jpg\" alt=\"\" width=\"240\" height=\"190\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCXCL1 (GRO alpha) is a member of the CXC chemokine family. CXCL1 contains an ELR motif and preferentially attracts neutrophils. Human CXCL1 is post translationally modified and divided into three isoforms, known as CXCL1 (4-73), CXCL1 (5-73), and CXCL1 (6-73). These isoforms are produced by proteolytic cleavage after secretion by peripheral blood mononuclear cells, and their in vitro activities are higher than those of the full-length protein. The other two CXC proteins cxcl2 (or gro beta) and cxcl3 (or gro\u0026amp; gamma;)It has 90% and 86% amino acid sequence homology with CXCL1, respectively. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes, and osteocytes. In addition, CXCL1 mRNA has been detected in breast fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth regulatory gene that is constitutively overexpressed in tumorigenic cells. As a result, elevated levels of CXCL1 can be found in several types of tumors and cancers, including lung cancer and melanoma.\u003cbr\u003eCXCL1 is a member of the CXC family of chemokines. Chemokines play a role in normal and pathological processes, including anaphylaxis, angiogenesis, inflammation, tumor growth, and metastasis. Mouse cxcl2 and cxcl3 share 67% and 60% sequence homology with mouse CXCL1, respectively. In addition, rat CXCL1 has 89% homology with mouse CXCL1.\u003c\/p\u003e\n\u003cp\u003eHomologues of IL-8 have long been identified in other species, such as sheep (Yoshimura and Johnson, 1993), rabbits (Yoshimura and yuhki, 1991), pigs (Goodman et al., 1992) and dogs (Ishikawa et al., 1993), but so far, homologues of IL-8 protein in mice and rats have not been clearly defined. At present, MIP-2 (ccl8) and KC (CXCL1) are widely believed to be the functional homologs of IL-8 protein in mice\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518154186827,"sku":"abs520017-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/32fdd8681a204baea89aea34a6c6bf3c.jpg?v=1792756829"},{"product_id":"cxcl2-elisa-kit","title":"Mouse CXCL2\/MIP-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse cxcl2\/mip-2 antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, cxcl2\/mip-2 in the sample is combined with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse \u0026amp;nbspcxcl2\/mip-2one copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003esee the instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003cstrong\u003e96-103%\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan lang=\"EN-US\" style=\"font-size: 10pt; font-family: 微软雅黑, sans-serif;\"\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520018.jpg\" alt=\"\" width=\"240\" height=\"187\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cimg src=\"%E2%80%9Chttps:\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/images\/upload\/Image\/520018.jpg\" width=\"\" alt=\"\" height=\" 187 \"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eChemokine ligand 2 (cxcl2) is a small cytokine belonging to the CXC chemokine family, also known as macrophage inflammatory protein 2-\u0026amp; alpha; (mip2 alpha), growth regulatory protein \u0026amp; amp; beta; (GRO beta) and gro oncogene - 2 (gro-2), cxcl2 \/ gro beta, also known as MIP-2 in mice and cinc-3 in rats, is a member of the CXC chemokine family. Cxcl2 has 90% homology with CXCL1 in amino acid sequence. This chemokine is secreted by monocytes and macrophages and has a chemotactic effect on polymorphonuclear leukocytes and hematopoietic stem cells. The gene for cxcl2 is located on human chromosome 4 in other CXC chemokine clusters. Cxcl2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.\u003cbr\u003eThe length of human cxcl2 \/ gro beta is 107 amino acids (AA) and the estimated molecular weight is 11 kDa. The mouse and rat orthologs share 70% and 71% amino acid sequence identity with the human protein, respectively. The N-terminal amino acids 1-4 of cxcl2 \/ gro beta can be post translationally cleaved, thereby enhancing hematopoietic biological activity. Cxcl2 \/ gro beta is produced by a variety of cell types, including monocytes and macrophages at the site of inflammation, and has chemotactic effects on granulocytes, including neutrophils.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518154612811,"sku":"abs520018-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/dcb38131440f4c16a555538287a6cff6.jpg?v=1792756832"},{"product_id":"mouse-ccl3-mip-1-alpha-elisa-kit","title":"Mouse CCL3\/MIP-1α ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti mouse mip-1α The monoclonal antibody was coated on the microplate, and mip-1\u0026amp;alpha in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse mip-1\u0026amp;alpha was added; Polyclonal antibody, and mip-1\u0026amp;alpha bound on the microplate; Combine to form immune complexes, and the free components are washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plates, standards, anti mouse mip-1α One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.5 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81 - 500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85.0-111.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003cp\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20230111\/193dc8378f924ba19b5cb85977c07efe.png\" width=\"300\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily, which was initially purified from the conditioned medium of LPS stimulated murine macrophage cell lines. Mip-1\u0026amp; alpha; is highly effective on a variety of cells (including monocytes, T cells, B cells, and eosinophils)Plays a chemotactic role.\u003cbr\u003etwo human mip-1α Genes are generated by duplication \/ mutation. They encode mip-1α Isoforms CCL3 \/ ld78a and CCL3L1 \/ ld78b, which share 94% amino acid sequence homology. Human CCL3 \/ ld78a is a single copy gene, while human CCL3L1 \/ ld78b gene copy number varies among populations. Human CCL3L1 \/ ld78b binds and signals through chemokine receptors CCR1, CCR5. Compared with CCL3 \/ ld78a, CCL3L1 \/ ld78b has higher binding affinity for CCR5, which is also a co receptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518154645579,"sku":"abs520019-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9fcaa20da5dc423a919d235a4bcefe35.jpg?v=1792753367"},{"product_id":"mouse-il-elisa-kit-abs520009","title":"Mouse IL-17 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.88pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific mouse il-17antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-17present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96orifice plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96well plates, standards, anti mouse il-17detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range: \u003c\/strong\u003e15.6 - 1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery range: \u003c\/strong\u003e82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMouse interleukin 17(il-17, also known as il-17a, or ctla-8), is a 21 kDa glycosylated polypeptide of varying degrees, belonging to the il-17 family. Cytokines of this family all contain a fold of a cysteine knot. People initially isolated il-17from activated hybridoma cells fused by cytotoxic cells in mice and t lymphocytes in rats. The mouse il-17 precursor consists of 158 amino acids, including a 25 amino acid signal peptide and a 133 amino acid mature peptide of 15kda . Both human and mouse il-17 have a conserved n-terminal glycosylation site, which is about 5kdaIL-17A can form a homodimer of 35-38kda or a heterodimer of 45-48 with il-17f . Mature mouse il-17a has 61%and 89%amino acid homology with human and rat il-17a , respectively. Although rodent and human mature IL-17A sequences share only a certain degree of homology, human IL-17 is also active on cells from mice and rats. The cells secreting il-17 include cd4+ Th17 T cells, Paneth cells, gr1+cd11b+ myelosuppressor cells, cd27-\u0026amp; Gamma\u0026amp;Amp; Delta; T cells, cd1+ nk11-ink T cells are the same as cd3-cd4+ LTI.\u003c\/p\u003e\n\u003cp\u003eThe high affinity receptor of mouse il-17 has been reported. It is a heteromultimer of il-17 RA and il-17rc , with a composition ratio of 2:1il-17 RA is a i type transmembrane glycoprotein of 130 kDa and has no similarity to il-17, tumor necrosis factor or immunoglobulin receptor superfamily membersil-17 RC is a itype transmembrane protein, about 90-95 kda, and the amino acid sequence homology with il-17 RA is only 30%. Both receptors are necessary for the activity of il-17a and il-17 a: f . When the ligand binds to il-17ra , the two receptors subsequently form functional binding.\u003c\/p\u003e\n\u003cp\u003eIL-17 is mainly involved in neutrophil recruitment and survival. Its production was first thought to be the result of antigen stimulation of dendritic cells, leading to the secretion of il-23 . In the case of non t cell receptor dependence, il-23 will stimulate t cells to secrete il-17. The secreted il-17 in bone marrow stimulates stromal and fibroblasts to express g-csf and membrane stem cell factor, thereby increasing the differentiation and formation of PMNs il-17 plays a complementary role by blocking the apoptosis of neutrophils and promoting the circulation of pmn in vivo. In tissues, il-17 can promote the extravasation of neutrophils mainly through its effects on macrophages and endothelial cells. In macrophages, il-17 can induce tnf-\u0026amp; Alpha, il-1\u0026amp; Betaand il-6 productiontnf-\u0026amp; Alphaand il-1\u0026amp; Betareacts on endothelial cells and promotes the secretion of g-csf, which is enhanced by il-17 . By inducing the release of cxc chemokines and nitric oxide from endothelial cells, il-17 plays a role in promoting pmn influx pooling, thereby improving vascular permeabilitythe effect of IL-17 is not limited to neutrophils, and at the joint site, il-17 can upregulate the expression of rankl in osteoclasts. This promotes the formation of osteoclasts and subsequent bone resorption.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41678624522315,"sku":"abs520009-96T","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2818824578a24f0cac04e15209719073.jpg?v=1792753522"}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-elisa-kits-mouse-elisa-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}