{"title":"General ELISA Kits","description":null,"products":[{"product_id":"human-porcine-insulin-elisa-kit","title":"Human \/ Porcine Insulin ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti- Human \/ Porcine Insulin antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the Human \/ Porcine Insulin present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eS\u003cspan style=\"color: #000000;\"\u003eample \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e   2.15 pmol \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1000 pmol\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e   85.0-108.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/c36b0514d4ff44bc8a1eaf4cf421cf28.png\" alt=\"\" width=\"345\" height=\"273\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: Insulin\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInsulin and IGF are members of the insulin molecule family. IGF-I and II are structurally homologous to proinsulin and can be thought of as an exaggerated inverted G shape. The G part of the chain is inchain disulfide bonded. Single-stranded proinsulin peptide is composed of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589) and 21 amino acid residues of A chain (aa 90110).The removal of C peptide by proteolysis results in the formation of mature insulin, which is the heterodimers formed by disulfide bonds between A and B chains. Circulating C peptide levels are elevated in hyperinsulinemia, obesity and type 2 diabetes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical hints: \u003c\/strong\u003eFor research use only, not for in vitro diagnosis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305774155,"sku":"abs510030-96T","price":450.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-elisa-kits-general-elisa-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}