{"title":"Cell Detection Kits","description":null,"products":[{"product_id":"annexin-v-fitc-apoptosis-assay-kit","title":"Annexin V-FITC\/PI Apoptosis Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating Instructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e(1) Centrifuge at low speed before use to avoid liquid accumulation in the tube cover and tube wall; \n(2) This reagent is only suitable for experiments, not for clinical testing; \n(3) PI (propidium iodide, propidium bromide) is toxic, can be absorbed through the skin, and has an irritating effect on the eyes. Wear gloves when using it; \n(4) Annexin V-FITC and PI are photosensitive substances, please pay attention to avoid light during operation. When handling and marking, do it in the dark as much as possible. During the incubation phase, the container is wrapped with aluminum foil or placed in a drawer. After the cells were labeled, they were observed with a microscope in a dark room; \n(5) The whole operation process should be as gentle as possible, do not blow the cells hard, and try to operate at 4 ℃ to avoid affecting the cell state. \n(6) In the last step of cell washing, please discard the supernatant as much as possible to avoid PBS residue, which may affect the experimental results. \n(7) In order to prevent fluorescence decay, flow cytometry detection should be performed within 1 hour. \n(8) If the PI staining time is too long, it may cause the detected apoptosis rate to be high. It is recommended to perform Annexin V-FITC staining first, and PI staining can be added at least 5 minutes before the machine. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-FITC apoptosis assay kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtocol\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdherent cells need to be digested with 0.25% trypsin. Note that over-digestion can damage cells. 2% BSA can be added during digestion to prevent overdigestion. If digested with trypsin containing EDTA, be careful that EDTA must be completely removed: wash with 1 × PBS or 1 × binding buffer before labeling to remove EDTA to prevent residual EDTA from chelating with Ca2 + and affecting the binding of Annexin V. \n(1) Dilute 10 × Binding Buffer into 1 × Binding Buffer with deionized water; \n(2) Cell collection: suspension cell collection: centrifugation for 5 minutes; Adherent cells: After digestion and collection with trypsin without EDTA (Note: the trypsin digestion time should not be too long, otherwise it will affect the binding of phosphatidylserine and Annexin V-FITC on the cell membrane), centrifuge at 2000 rpm at room temperature for 5 ~ 10 minutes to collect the cells; \n(3) Cell washing: Resuspend the cells once with pre-cooled 1 × PBS (4 ℃), centrifuge at 2000 rpm for 5-10 minutes, and discard the supernatant to wash the cells; \n(4) 300 μL of 1 × Binding Buffer suspension cells were added; \n(5) Annexin V-FITC labeling: Add 5μL of Annexin V-FITC and mix well, protect from light, and incubate at room temperature for 15 minutes; \n(6) PI labeling: Add 5μL of PI staining 5 minutes before loading the machine. \n(7) Before loading, add 200 μL of 1 × Binding Buffer. \nNote: The recommended number of cells per reaction system is 1 × 10 \u003csup\u003e4\u003c\/sup\u003eTo 1 × 10\u003csup\u003e6\u003c\/sup\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e In normal cells, phosphatidylserine is only distributed on the inner side of the cell membrane lipid bilayer. At the earliest stage of cell apoptosis, membrane phosphatidylserine ( PS ) from the inside to the outside of the lipid membrane, this change is earlier than cell shrinkage, chromatin condensation, DNA Apoptotic phenomena such as fragmentation and increased permeability of cell membrane. Annexin V It is a phospholipid-binding protein with high affinity for phosphatidylserine, so it can bind to the cell membrane of early apoptotic cells through the exposed phosphatidylserine on the outside of the cell. Hence Annexin V It is used as one of the sensitive indicators to detect early apoptosis of cells. Propidium iodide ( Propidium Iodide, PI ) is a nucleic acid dye ,  It cannot penetrate the intact cell membrane, but cells and dead cells in the middle and late stages of apoptosis due to the increase of cell membrane permeability, PI  Can penetrate the cell membrane and make the nucleus red. Thus will Annexin V  With PI  With matching, cells in different apoptotic stages can be distinguished. \u003cbr\u003e\u003cstrong\u003e Product composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 88px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.238%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.5246%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e25T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.9144%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e50T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.295%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.238%; text-align: center; height: 22px;\"\u003eAnnexinV-FITC\u003c\/td\u003e\n\u003ctd style=\"width: 18.5246%; text-align: center; height: 22px;\"\u003e125μL\u003c\/td\u003e\n\u003ctd style=\"width: 21.9144%; text-align: center; height: 22px;\"\u003e250μL\u003c\/td\u003e\n\u003ctd style=\"width: 19.295%; text-align: center; height: 22px;\"\u003e500μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.238%; text-align: center; height: 22px;\"\u003e10×Binding Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 18.5246%; text-align: center; height: 22px;\"\u003e1.5mL\u003c\/td\u003e\n\u003ctd style=\"width: 21.9144%; text-align: center; height: 22px;\"\u003e2.5mL\u003c\/td\u003e\n\u003ctd style=\"width: 19.295%; text-align: center; height: 22px;\"\u003e6mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.238%; text-align: center; height: 22px;\"\u003ePropidium Iodide\u003c\/td\u003e\n\u003ctd style=\"width: 18.5246%; text-align: center; height: 22px;\"\u003e125μL\u003c\/td\u003e\n\u003ctd style=\"width: 21.9144%; text-align: center; height: 22px;\"\u003e250μL\u003c\/td\u003e\n\u003ctd style=\"width: 19.295%; text-align: center; height: 22px;\"\u003e500μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 °C for 6 months; Avoid repeated freezing and thawing.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25T","offer_id":41683110821963,"sku":"abs50001-25T","price":143.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683110854731,"sku":"abs50001-50T","price":206.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521807687755,"sku":"abs50001-100T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-counting-kit","title":"CCK-8 Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCCK8,CCK 8,Cell Counting Kit-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eCell Counting Kit 8, referred to as CCK-8 kit for short, is a highly sensitive, non-radioactive colorimetric detection method used to determine the number of living cells in cell proliferation or toxicity experiments.\u003c\/p\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eCCK-8 is an upgraded product of MTT. CCK-8 solution can be directly added to cell samples without pre-preparing various ingredients. It has rapid detection and very low toxicity. CCK-8 is based on the water-soluble tetrazolium salt WST-8. The working principle is: WST-8 can be reduced by dehydrogenase in mitochondria to produce orange-yellow formazan dye in the presence of electron coupling reagent. The formazan dye is able to dissolve in tissue culture medium in proportion to the number of viable cells. Through colorimetry, the number of viable cells can be dynamically quantified, so as to detect cell proliferation or drug toxicity. This kit is usually used for cycle and apoptosis detection of adherent or suspended cells. If it is used in tissue, the tissue needs to be digested into a single-cell state, and then it can be tested.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is restricted to scientific purposes only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is effective for one year when stored at 4 ℃ in the dark, and for two years when stored at-20 ℃ in the dark.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500T","offer_id":43115013406795,"sku":"abs50003-500T","price":107.0,"currency_code":"USD","in_stock":true},{"title":"5000T","offer_id":41683118751819,"sku":"abs50003-5000T","price":714.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-cycle-assay-kit","title":"Cell Cycle Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSelf-provided consumables and equipment:\u003cbr\u003e1 ml pipette\u003cbr\u003e100-200 μl pipette\u003cbr\u003eRotary socket mixer\u003cbr\u003eFlow cytometry\u003cbr\u003ePBS\u003cbr\u003e75% ethanol\u003cbr\u003e\u003cbr\u003eApplicable experiment and operation process:\u003cbr\u003e1. Cell preparation Adherent cells: Discard the cell culture medium, digest with trypsin, prepare a single cell suspension, centrifuge at 1000 rpm for 5 minutes, and discard the supernatant. The pellet was resuspended with 1 ml of pre-cooled PBS. Centrifuge again at 1000 rpm for 5 minutes and discard the supernatant. Suspended cells: The cell suspension was collected, centrifuged at 1000 rpm for 5 minutes, and the supernatant was discarded. The pellet was resuspended with 1 ml of pre-cooled PBS. Centrifuge again at 1000 rpm for 5 minutes and discard the supernatant. Histiocytes: After cutting the tissue pieces into the smallest possible pieces with scissors, digested with 0.25% trypsin for 0.5-1 hour. Filtering through a 200-400 mesh screen to obtain a single cell suspension. Centrifuge at 1000 rpm for 5 minutes, discard the supernatant, and resuspend the pellet with 1 ml of pre-cooled PBS. Centrifuge again at 1000 rpm for 5 minutes and discard the supernatant. Note that for the last centrifugation, discard the supernatant and leave 50 μl of the supernatant, vortex and mix well.\u003cbr\u003e2. Cell fixation The cell pellet is gently mixed with 1 ml-20 °C pre-cooled 75% ethanol, and fixed at 4 °C for more than 2 hours or overnight. Then, centrifuge at 1000 rpm for 5 minutes, gently discard the supernatant, resuspend the pellet with 1 ml of pre-cooled PBS, centrifuge at 1000 rpm for 5 minutes, discard the supernatant.\u003cbr\u003e3. Staining PI staining working solution configuration: Add 25μl PI staining solution (solution A) and 10μl RNase A (solution B) to 0.5 ml staining buffer solution (solution C), mix well and set aside. Add 0.5 ml of the prepared PI staining working solution to each cell sample, and gently mix and resuspend the cells. Incubate at 37 ℃ for 30 minutes in the dark from light, and detect directly by flow cytometry (it is preferable to complete within 5 hours). The excitation wavelength was 488 nm and red fluorescence was detected. Note: Not more than 1 × 10 cells per test\u003csup\u003e6\u003c\/sup\u003eOne.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCell cycle and apoptosis detection kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThis product is based on Propidium iodide (PI) staining method to analyze cell cycle and apoptosis. Propidium iodide is a double-stranded DNA dye. Its intercalation in double-stranded DNA can produce fluorescence. The intensity of fluorescence is directly proportional to the amount of double-stranded DNA.\u003cbr\u003eIn the normal cell cycle, there is one set of chromosomes in G0 and G1 phases, two sets of chromosomes in G2 and M phases cells, and the S phase is in between. After PI staining, the fluorescence intensity of cells in different cell cycles is different. Assuming that the fluorescence intensity of cells in G0 and G1 phases is 1, then the fluorescence intensity of cells in G2 and M phases is 2, and the cells in S phase are between 1 and 2.\u003cbr\u003eDuring apoptosis, due to nucleus shrinkage and DNA fragmentation, DNA fragments will be lost from the perforated cell membrane during staining. When detected by flow cytometry, the fluorescence intensity is less than 1, that is, Sub-G1 peak or apoptotic cell peak.\u003cbr\u003eApoptosis can also be detected by observing changes in cell light scattering by flow cytometry. In the early stage of apoptosis, chromatin shrinks, cell density increases, and forward angle light dispersion decreases significantly. In the later stage of apoptosis, cells produce apoptotic bodies, and both forward angular light scattering and lateral angular light scattering are significantly reduced.\u003cbr\u003e\u003cbr\u003eProduct Components:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 47.6576%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.7955%; text-align: center;\"\u003eserial number\u003c\/td\u003e\n\u003ctd style=\"width: 19.0183%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 11.0599%; text-align: center;\"\u003e50T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.7955%; text-align: center;\"\u003eA\u003c\/td\u003e\n\u003ctd style=\"width: 19.0183%; text-align: center;\"\u003ePropidium Iodide (20 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 11.0599%; text-align: center;\"\u003e1.25 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.7955%; text-align: center;\"\u003eB\u003c\/td\u003e\n\u003ctd style=\"width: 19.0183%; text-align: center;\"\u003eRNase A (50 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 11.0599%; text-align: center;\"\u003e500 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.7955%; text-align: center;\"\u003eC\u003c\/td\u003e\n\u003ctd style=\"width: 19.0183%; text-align: center;\"\u003eStaining Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 11.0599%; text-align: center;\"\u003e25ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. PI is toxic. Pay attention to protection during operation, protect eyes, avoid inhalation, and wear disposable gloves.\u003cbr\u003e2. PI has quenching phenomenon, so avoid light during storage and use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at-20 ℃ in the dark from light, shelf life is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is used for the detection of cultured adherent cells or suspended cells, and can also be used for the detection of tissue cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"50T","offer_id":41518126694475,"sku":"abs50005-50T","price":100.0,"currency_code":"USD","in_stock":true}]},{"product_id":"annexin-v-egfp-pi-apoptosis-analysis-kit","title":"Annexin V-EGFP\/PI Apoptosis analysis kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Adjust the cell density to about 1 × 10\u003csup\u003e6\u003c\/sup\u003eCells\/ml. 2. Remove 0.2 ml of cell suspension from a cell culture flask (2 × 10\u003csup\u003e5\u003c\/sup\u003eCells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0.2 ml of pre-cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell pellet was gently resuspended with 0.2 ml of pre-cooled binding buffer (component C. Binding Buffer). 7. Add 5μl Annexin V-EGFP (component A) and 5μl PI dye solution (component B), and gently mix well. 8. Incubate at room temperature (18-24 °C) for 10-15 minutes in the dark. 9. Alternatively, replenish the volume to 0.4 ml with binding buffer or PBS. 10. Place the sample on ice and protect it from light, and test it as soon as possible. 11. Use flow cytometry or fluorescence microscope for detection and analysis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-EGFP\/PI Apoptosis Analysis Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAnnexin V-EGFP\/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis.\u003c\/p\u003e\n\u003cp\u003eThe detection principle is: in normal living cells, phosphotidylserine (PS) is located on the inside of the cell membrane, but in early apoptotic cells, PS flips from the inside of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V (Annexin-V) is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36KD, which can bind to PS with high affinity. It can bind to the cell membrane of early apoptotic cells through phosphatidylserine exposed on the outside of the cell.\u003c\/p\u003e\n\u003cdiv\u003eIn addition, Propidium Iodide (PI) is also provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye that cannot penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells to make the nucleus red. Therefore, when Annexin V is used in combination with PI, PI is excluded from viable cells (Annexin V-\/PI-) and early apoptotic cells (Annexin V +\/PI-), while late apoptotic cells and necrotic cells are stained double positive by EGFP and PI combination (Annexin V +\/PI +).\u003c\/div\u003e\n\u003cdiv\u003eProduct Components:\u003c\/div\u003e\n\u003cdiv\u003e\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"565\" height=\"112\" style=\"border-collapse:collapse;width:282.52pt;\"\u003e\u003ctbody\u003e\n\u003ctr height=\"28\" style=\"height:14.00pt;\"\u003e\n\u003ctd class=\"et3\" height=\"28\" width=\"82\" style=\"height:14.00pt;width:41.00pt;\"\u003eserial number\u003c\/td\u003e\n\u003ctd class=\"et4\" width=\"207\" style=\"width:103.50pt;\"\u003eName\u003c\/td\u003e\n\u003ctd class=\"et4\" width=\"145\" style=\"width:72.50pt;\"\u003e20T\u003c\/td\u003e\n\u003ctd class=\"et4\" width=\"131\" style=\"width:65.50pt;\"\u003e50T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr height=\"28\" style=\"height:14.00pt;\"\u003e\n\u003ctd class=\"et4\" height=\"28\" style=\"height:14.00pt;\"\u003eA\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003eAnnexin V-EGFP\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e100ul\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e250ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr height=\"28\" style=\"height:14.00pt;\"\u003e\n\u003ctd class=\"et3\" height=\"28\" style=\"height:14.00pt;\"\u003eB\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003ePI\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e100ul\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e250ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr height=\"28\" style=\"height:14.00pt;\"\u003e\n\u003ctd class=\"et4\" height=\"28\" style=\"height:14.00pt;\"\u003eC\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003eBinding Buffer\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e5ml\u003c\/td\u003e\n\u003ctd class=\"et4\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2-8 ℃ in the dark from light, the validity period is 6 months. Store at-20 ℃ in the dark from light, shelf life for 12 months or more, avoid repeated freezing and thawing.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683111018571,"sku":"abs50006-20T","price":143.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41521809424459,"sku":"abs50006-50T","price":206.0,"currency_code":"USD","in_stock":true}]},{"product_id":"annexin-v-pe-7-aad-apoptosis-analysis-kit","title":"Annexin V-PE\/7-AAD apoptosis analysis kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Instrument parameter adjustment\u003cbr\u003e1. Collect 1 × 10\u003csup\u003e6\u003c\/sup\u003e-3×10\u003csup\u003e6\u003c\/sup\u003eThe cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded.\u003cbr\u003e2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes.\u003cbr\u003e3. Wash by centrifugation with pre-cooled PBS and discard the supernatant.\u003cbr\u003e4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes.\u003cbr\u003e5. Add 5 uL of Annexin V-PE or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light.\u003cbr\u003e6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube\u003cbr\u003eCompensation of channels.\u003cbr\u003e\u003cstrong\u003eNote:\u003c\/strong\u003eCertain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase.\u003cbr\u003eThe adherent cells can be cultured on a suitable vessel, such as a sterile glass slide, and stained with suitably modified methods. However, this method has not been tested in our laboratory.\u003cbr\u003e\u003cbr\u003e2. Sample testing\u003cbr\u003e1. Induce apoptosis according to the experimental protocol.\u003cbr\u003e2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 10\u003csup\u003e5\u003c\/sup\u003eCells include cells in the culture supernatant. Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells.\u003cbr\u003e3. Add 5 uL Annexin V-PE and 10 uL 7-AAD per tube.\u003cbr\u003e4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes.\u003cbr\u003e5. Get on the computer for analysis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-PE\/7-AAD Apoptosis Analysis Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAnnexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V\u003cbr\u003eCan specifically bind to it, indicating that the cell is an apoptotic cell. This kit takes only 15 minutes to stain, which is quick and easy.\u003c\/p\u003e\n\u003cp\u003eKit Components:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 73.4507%; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.3148%; text-align: center;\" width=\"160\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 14.2415%; text-align: center;\" width=\"64\"\u003e25T\u003c\/td\u003e\n\u003ctd style=\"width: 12.6612%; text-align: center;\" width=\"68\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.3148%;\" width=\"160\"\u003eAnnexin V-PE\u003c\/td\u003e\n\u003ctd style=\"width: 14.2415%;\"\u003e150uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.6612%;\"\u003e500uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.3148%;\" width=\"160\"\u003e7-AAD\u003c\/td\u003e\n\u003ctd style=\"width: 14.2415%;\"\u003e300uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.6612%;\"\u003e1mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.3148%;\" width=\"160\"\u003e5 × Binding buffer\u003c\/td\u003e\n\u003ctd style=\"width: 14.2415%;\"\u003e5mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.6612%;\"\u003e15mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.3148%;\" width=\"160\"\u003eApoptosis Positive Control Solution\u003c\/td\u003e\n\u003ctd style=\"width: 14.2415%;\"\u003e5mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.6612%;\"\u003e5mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic.\u003cbr\u003e2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2-8 °C, do not freeze, shelf life 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25T","offer_id":41683111051339,"sku":"abs50007-25T","price":143.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521809522763,"sku":"abs50007-100T","price":254.0,"currency_code":"USD","in_stock":true}]},{"product_id":"annexin-v-apc-7-aad-apoptosis-analysis-kit","title":"Annexin V-APC\/7-AAD apoptosis analysis kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Instrument parameter adjustment\u003cbr\u003e1. Collect 1 × 10\u003csup\u003e6\u003c\/sup\u003e-3×10\u003csup\u003e6\u003c\/sup\u003eThe cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded.\u003cbr\u003e2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes.\u003cbr\u003e3. Wash by centrifugation with pre-cooled PBS and discard the supernatant.\u003cbr\u003e4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes.\u003cbr\u003e5. Add 5 uL of Annexin V-APC or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light.\u003cbr\u003e6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube\u003cbr\u003eCompensation of channels.\u003cbr\u003e\u003cstrong\u003eNote:\u003c\/strong\u003eCertain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase.\u003cbr\u003e\u003cbr\u003e2. Sample testing\u003cbr\u003e1. Induce apoptosis according to the experimental protocol.\u003cbr\u003e2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 10\u003csup\u003e5\u003c\/sup\u003eCells (including cells in the culture supernatant). Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells.\u003cbr\u003e3. Add 5 uL of Annexin V-APC and 10 uL of 7-AAD per tube.\u003cbr\u003e4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes.\u003cbr\u003e5. Get on the computer for analysis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-APC\/7-AAD Apoptosis Analysis Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAnnexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V\u003cbr\u003eCan specifically bind to it, indicating that the cell is an apoptotic cell. This kit takes only 15 minutes to stain, which is quick and easy.\u003c\/p\u003e\n\u003cp\u003eKit Components:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 73.4506%; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.5676%; text-align: center;\" width=\"160\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 12.8999%; text-align: center;\" width=\"64\"\u003e25T\u003c\/td\u003e\n\u003ctd style=\"width: 12.8829%; text-align: center;\" width=\"68\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.5676%;\" width=\"160\"\u003eAnnexin V-APC\u003c\/td\u003e\n\u003ctd style=\"width: 12.8999%;\"\u003e150uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.8829%;\"\u003e500uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.5676%;\" width=\"160\"\u003e7-AAD\u003c\/td\u003e\n\u003ctd style=\"width: 12.8999%;\"\u003e300uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.8829%;\"\u003e1mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.5676%;\" width=\"160\"\u003e5 × Binding buffer\u003c\/td\u003e\n\u003ctd style=\"width: 12.8999%;\"\u003e5mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.8829%;\"\u003e15mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.5676%;\" width=\"160\"\u003eApoptosis Positive Control Solution\u003c\/td\u003e\n\u003ctd style=\"width: 12.8999%;\"\u003e5mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.8829%;\"\u003e5mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic.\u003cbr\u003e2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2-8 °C, do not freeze, shelf life 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25T","offer_id":41683111084107,"sku":"abs50008-25T","price":143.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521810669643,"sku":"abs50008-100T","price":317.0,"currency_code":"USD","in_stock":true}]},{"product_id":"annexin-v-apc-pi-apoptosis-analysis-kit","title":"Annexin V-APC\/PI apoptosis analysis kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Self-prepared instruments and reagents:\u003c\/strong\u003e\u003cbr\u003e1. Reagent: PBS (abs962); EDTA-free trypsin digestion (abs47014937, abs47048223)\u003cbr\u003e2. Consumables and instruments: 1.5 mL centrifuge tube (sterile and enzyme-free) (abs7119), flow cytometer, fluorescence microscope, low-speed centrifuge, micropipette.\u003cbr\u003e\u003cstrong\u003e2. Operation steps:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eFor streaming operation detection\u003c\/strong\u003e\u003cbr\u003e1. Cell collection\u003cbr\u003e(1) Suspended cells: 300 × g centrifugal force, centrifugation for 5 min to collect;\u003cbr\u003e(2) Adherent cells: Adherent cells are collected by trypsin digestion without EDTA (Note: the trypsin digestion time should not be too long, otherwise it will easily cause false positives);\u003cbr\u003e2. Wash the cells twice with PBS (300 × g, centrifuge for 5 min) and collect 1-5 × 10\u003csup\u003e5\u003c\/sup\u003eCells;\u003cbr\u003e3. Add 500μL of Binding Buffer and gently blow to form a single cell suspension;\u003cbr\u003e4. Add 5μL Annexin V-APC and 5μL Propidium Iodide, and gently mix;\u003cbr\u003e5. React for 5-10 min at room temperature and in the dark from light;\u003cbr\u003e6. Please perform flow cytometer observation and detection within 1 hour.\u003cbr\u003e7. Flow cytometry analysis\u003cbr\u003e(1) Detection by flow cytometry, fluorescence channel (FL4) of Annexin V-APC (Ex = 633 nm, Em = 660 nm); PI red fluorescence (Ex = 488 nm, Em ≥ 630 nm) was detected by PE channels (FL2 or FL3).\u003cbr\u003e(2) Fluorescence compensation adjustment: Using induction-treated apoptotic cells as a control, fluorescence compensation adjustment was performed to remove spectral overlap and set the position of cross gates.\u003cbr\u003e\u003cstrong\u003eFor fluorescence microscopy detection\u003c\/strong\u003e\u003cbr\u003e1. Select a 24-well plate or a 48-well plate for cell culture and treatment;\u003cbr\u003e2. Before staining, use a multi-well plate centrifuge 300 × g for 5 min;\u003cbr\u003e3. Remove the cell culture medium and add PBS to wash once;\u003cbr\u003e4. According to the number of samples, calculate and prepare the staining working solution: in a 1.5 mL EP tube, add 500μL Binding Buffer, 5μL Annexin V-APC and 5μL Propidium Iodide, and mix well (take 500μL as an example);\u003cbr\u003e5. Add the staining working solution to the well plate (500μL per well for the 12-well plate, 200μL per well for the 24-well plate, 100μL per well for the 96-well plate), and incubate at room temperature in the dark for 20 minutes;\u003cbr\u003e6. Observe immediately under a fluorescence microscope.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-APC\/PI Apoptosis Analysis Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIn normal cells, phosphatidylserine (PS) is only distributed on the inside of the lipid bilayer of the cell membrane, while in the early stage of apoptosis, phosphatidylserine (PS) in the cell membrane turns from the inside to the outside of the lipid membrane. Annexin V is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36 kD. It has a high affinity for phosphatidylserine, so it can bind to the cell membrane of early apoptotic cells through the exposed phosphatidylserine on the outside of the cell. Therefore, Annexin V is one of the sensitive indicators to detect early apoptosis. Annexin V was labeled with fluorescein APC, and the labeled Annexin V was used as a fluorescent probe to detect the occurrence of apoptosis by fluorescence microscope or flow cytometry.\u003cbr\u003ePropidium Iodide (PI) is a nucleic acid dye that cannot penetrate the intact cell membrane, but for cells in the middle and late stages of apoptosis and dead cells, PI can penetrate the cell membrane and make the nucleus red. Therefore, matching Annexin V-APC with PI can distinguish cells in different apoptotic stages.\u003cbr\u003eThis kit can be applied to the apoptosis detection of cultured cells (not recommended for the detection of tissue samples).\u003cbr\u003eProduct composition:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 80.3563%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.2656%; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 13.2911%; text-align: center;\"\u003e20T\u003c\/td\u003e\n\u003ctd style=\"width: 12.9085%; text-align: center;\"\u003e50T\u003c\/td\u003e\n\u003ctd style=\"width: 12.5258%; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003ctd style=\"width: 14.6941%; text-align: center;\"\u003eSave\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.2656%; text-align: center;\"\u003eAnnexin V-APC\u003c\/td\u003e\n\u003ctd style=\"width: 13.2911%; text-align: center;\"\u003e100uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.9085%; text-align: center;\"\u003e250uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.5258%; text-align: center;\"\u003e500uL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6941%; text-align: center;\"\u003e2-8 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.2656%; text-align: center;\"\u003ePropidium Iodide\u003c\/td\u003e\n\u003ctd style=\"width: 13.2911%; text-align: center;\"\u003e100uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.9085%; text-align: center;\"\u003e250uL\u003c\/td\u003e\n\u003ctd style=\"width: 12.5258%; text-align: center;\"\u003e500uL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6941%; text-align: center;\"\u003e2-8 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.2656%; text-align: center;\"\u003eBinding Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 13.2911%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.9085%; text-align: center;\"\u003e25mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.5258%; text-align: center;\"\u003e50mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6941%; text-align: center;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please centrifuge and collect the solution before taking the trace reagent.\u003cbr\u003e2. Annexin V-APC, Propidium Iodide (PI) is stored and used in the dark from light.\u003cbr\u003e3. Propidium Iodide (PI) is toxic, please wear gloves when operating.\u003cbr\u003e4. This kit is suitable for detecting living cells. When detected by flow cytometry, the number of cells should not be less than 1 × 10\u003csup\u003e5\u003c\/sup\u003eUnits\/mL, not recommended for testing tissue samples.\u003cbr\u003e5. Adherent cells need to be digested with trypsin without EDTA. If digested improperly, it may cause false positives, and using a cell scraper will cause cells to adhere and form clusters, which will affect the detection. Trypsinized cells can be stored in PBS containing 2% (w\/v) BSA to prevent further damage.\u003cbr\u003e6. The fluorescence may be quenched after cell fixation. Please do not fix the sample.\u003cbr\u003e7. Due to different types of detected cells, types of apoptosis inducers, detection instruments used, and different setting parameters, it is recommended that cells treated with apoptosis induction should be used as controls for each detection to adjust fluorescence compensation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKeep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25T","offer_id":43115016650827,"sku":"abs50009-25T","price":317.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43115016683595,"sku":"abs50009-100T","price":452.0,"currency_code":"USD","in_stock":true},{"title":"20T","offer_id":41683118522443,"sku":"abs50009-20T","price":151.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mtt-cell-proliferation","title":"MTT Cell Proliferation and Cytotoxicity Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Place the cell plate in the sterile operating table.\u003cbr\u003e2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg\/ml MTT solution. It can be used after preparation, or stored directly at-20 ℃ in the dark from light, or stored at-20 ℃ in the dark from light after appropriately packaging as needed.]\u003cbr\u003e3. Put the cell plate back into the incubator and continue to incubate for 3-4 hours. When doing comparative experiments, the incubation time should be consistent.\u003cbr\u003e4. After incubation, take out the cell plate from the incubator and dissolve it according to the following method to produce MTT formazan crystals.\u003cbr\u003e(1) If it is an adherent cell, discard the culture medium. Add the same amount of Formazan Solvent as the initial volume. The volume of dissolved fluid may vary per well, but the total volume must be consistent for comparison.\u003cbr\u003e(2) If the removal of non-adherent cells or culture medium will cause the loss of MTT formazan, add the same amount of lysate as the initial volume directly to the culture medium.\u003cbr\u003e(3) Readings must be completed within 1 hour after addition of Formazan Solvent.\u003cbr\u003e5. Gentle shaking in a shaker can enhance dissolution. In some cases, particularly when the cell density is high, repeated pipetting is required to completely dissolve the MTT formazan crystals.\u003cbr\u003e6. Spectrophotometer to measure OD570 and reference wavelength OD690.\u003cbr\u003e7. The 96-well plate can be detected on a microplate reader equipped with a suitable filter.\u003cbr\u003e8. Other types of multi-well plates need to be spectrophotometrically detected with a cuvette or plate reader of appropriate size.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMTT Cell Proliferation and Cytotoxicity Assay Kit,MTT kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThe MTT assay, also known as MTT colorimetry, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline Formazan and deposit it in cells, while dead cells do not have this function. Within a certain range of cell numbers, the amount of MTT crystals formed is directly proportional to the number of cells. The greater the absorbance, the stronger the cell activity, indicating that the drug toxicity is less. This method has been widely used in the activity detection of some bioactive factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination. It is characterized by high sensitivity and economy.\u003c\/p\u003e\n\u003cdiv\u003eComponents: MTT (25mg), MTT solvent (5ml), Formazan solution (55ml)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Since a 96-well plate is used for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since the circle around the 96-well plate is the easiest to evaporate, you can discard the circle around the plate and add PBS, water or culture medium instead; On the other hand, a 96-well plate can be placed close to a water source in the incubator to alleviate evaporation.\u003cbr\u003e2. If the test compound used can react with MTT, centrifuge first and then discard the culture medium. Carefully flush it with PBS for 2 to 3 times, and then add the culture medium containing MTT.\u003cbr\u003e3. The production of Formazan is not only proportional to the number of living cells, but also affected by the action time. Therefore, when there are many samples, the measurement results may change with time.\u003cbr\u003e4. This product contains carcinogens, avoid contact with skin and eyes, please operate carefully. For your safety and health, please operate in a lab coat and disposable gloves.\u003cbr\u003e5. This product is only used for scientific research and cannot be used clinically or diagnostically.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2 ~ 8 ℃, protected from light, valid for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500T","offer_id":41518128595019,"sku":"abs50010-500T","price":100.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-proliferation-assay-kit","title":"MTS Cell Proliferation Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental operation:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. MTS working fluid preparation:\u003c\/strong\u003e\u003cbr\u003eMix MTS Solution and PMS Solution at a ratio of 20: 1 to obtain the MTS working solution. If the prepared MTS working solution needs to be stored, it should be stored at-20 ℃ in the dark from light. At this time, it is recommended to dispense appropriate amounts to avoid repeated freezing and thawing.\u003cbr\u003eNote: Both MTS Solution and PMS Solution are easy to decompose when exposed to light, so they need to be protected from light during preparation and use (can be wrapped in tin foil). If you want to store this kit at 4 °C for frequent use, the MTS working solution should be prepared before each test. The prepared unused MTS working solution needs to be stored at-20 °C protected from light.\u003cbr\u003e\u003cstrong\u003e2. Cell culture:\u003c\/strong\u003e\u003cbr\u003eThe cells are seeded in a suitable container such as a well plate. For 96-well plates, it is recommended to add 100uL of medium per well, and it is recommended to inoculate 5-100 × 10 per well\u003csup\u003e3\u003c\/sup\u003eCells. The cells of the experimental group can be treated with the drug of interest for a period of time according to the experimental design, at which time it is necessary to set up a blank group (containing medium only) and a control group (containing medium, cells and drug solvent).\u003cbr\u003eNote: The specific number of cells seeded per well can be adjusted according to the specific cell type.\u003cbr\u003e\u003cstrong\u003e3. MTS incubation:\u003c\/strong\u003e\u003cbr\u003e20 uL of MTS working solution was added to each well and then incubated in a cell incubator for 1-4 h.\u003cbr\u003eNote: The MTS incubation time can be adjusted for specific experiments.\u003cbr\u003e\u003cstrong\u003e4. Detection:\u003c\/strong\u003e\u003cbr\u003eThe absorbance (A) was measured at 490 nm with a microplate reader. If a 490 nm filter is not available, optionally use a 450-540 nm filter.\u003cbr\u003e\u003cstrong\u003e5. Data analysis:\u003cbr\u003e\u003c\/strong\u003eThe cell survival rate is calculated as follows\u003cbr\u003eCell viability (%) = [(A\u003csub\u003eExperimental group\u003c\/sub\u003e-A\u003csub\u003eBlank group\u003c\/sub\u003e)\/(A\u003csub\u003eControl group\u003c\/sub\u003e-A\u003csub\u003eBlank group\u003c\/sub\u003e)] ×100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMTS Cell Proliferation Assay Kit (MTS Cell Proliferation Assay Kit) is a common kit for detecting cell proliferation and toxicity. MTS is a novel tetrazolium salt, which can be reduced by succinate dehydrogenase in mitochondria of living cells to the water-soluble product Formazan. Formazan is specifically absorbed at 490 nm, so cell proliferation can be reflected by measuring the absorbance at 490 nm. The faster the cell proliferation, the higher the absorbance value.\u003cbr\u003eCompared with the common MTT kit, this kit does not need to remove the original culture medium, nor does it need to use another solvent to dissolve formazan, which is more convenient to use, and can avoid experimental errors caused by taking away part of formazan when removing the culture medium. This kit is very flexible to use, and incubation can be continued after detection to obtain better color development results. In addition, the kit has the advantages of wide linear range, high sensitivity and safe use.\u003cbr\u003eProduct Components:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 52.5641%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e500T\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e2500T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003eMTS Solution\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003ePMS Solution\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e2.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. When the cells are cultured for a long time, the edge wells of the 96-well plate are prone to liquid evaporation. It is recommended to discard the circle around the 96-well plate and add sterile water, culture medium or PBS instead; At the same time, the 96-well plate can be placed in the incubator close to the water source.\u003cbr\u003e2. MTS Solution and PMS Solutin will solidify at low temperatures. Before use, please return to room temperature or 37 °C water bath for about 10 minutes until completely melted before use.\u003cbr\u003e3. MTS Solution and PMS Solution are sensitive to light. If stored at 4 °C, the color of the solution may change slightly. This change may increase the background absorbance value, but it will not affect the final experimental results.\u003cbr\u003e4. If it cannot be detected in time, you can add 25uL of 10% SDS to each well after the incubation in Step 3 to terminate the reaction. The SDS-treated well plate can be placed\u003cbr\u003eStore in an incubator protected from light for no more than 18 hours.\u003cbr\u003e5. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore the kit at -20°C away from light and moisture, stable for 1 year. For frequent use, the kit can be stored at 4°C away from light and moisture, stable for 6 weeks.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1000T","offer_id":41518128627787,"sku":"abs50011-1000T","price":357.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":41683113115723,"sku":"abs50011-500T","price":164.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":41683113148491,"sku":"abs50011-2500T","price":598.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mitochondrial-membrane-potential-detection-kit","title":"Mitochondrial membrane potential detection kit (JC-1)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 、 JC-1 Preparation of dyeing working solution \u003cbr\u003e Six-well plate required per well JC-1 The amount of dyeing working solution is 1mL , of other culture vessels JC-1 The amount of staining working solution used is similar: for the cell suspension per 50 ～ 100 Ten thousand cell needs 0.5mLJC-1 Dyeing Working Fluid. Take appropriate amount JC-1 （ 200× ), according to each 50μLJC-1 （ 200× ) join 8mL Proportional dilution of ultrapure water JC-1 。 Violently shake to fully dissolve and mix well JC-1 。 Then add 2mLJC-1 Staining buffer ( 5× ), after mixing, it is JC-1 Dyeing Working Fluid. \u003c\/p\u003e\n\u003cp\u003e2 Settings of Positive Control: \u003cbr\u003e Put the kit provided CCCP （ 10mM ) Recommended as per 1∶1000 The proportion is added to the cell culture solution, and diluted to 10μM , treating cells 20 Minutes. Subsequently loading as follows JC-1 , the mitochondrial membrane potential was detected. For most cells, typically 10μMCCCP dispose 20 After minutes, the membrane potential of mitochondria will be completely lost, JC-1 After staining, it should show green fluorescence; And normal cells through JC-1 Red fluorescence should be shown after staining. For specific cells, CCCP The concentration and duration of action may vary, so you need to refer to relevant literature to decide. \u003c\/p\u003e\n\u003cp\u003e3 For suspension cells, \u003cbr\u003e Take 10 ～ 60 Thousand cells, resuspended in 0.5mL In the cell culture medium, the cell culture medium may contain serum and phenol red. \u003cbr\u003e join 0.5mLJC-1 Dye the working solution, reverse it several times and mix well. In cell incubator 37℃ incubation 20 Minutes. \u003cbr\u003e During the incubation, according to each 1mLJC-1 Staining buffer ( 5× ) join 4mL Proportion of distilled water, prepare an appropriate amount JC-1 Staining buffer ( 1× ) and placed in an ice bath. \u003cbr\u003e37℃ After the incubation is completed, 600g4℃ Centrifugation 3 ～ 4 Min, the cells were pelleted. Discard the supernatant and be careful not to aspirate the cells as much as possible. \u003cbr\u003e use JC-1 Staining buffer ( 1× ) washing 2 Time: Join 1mLJC-1 Staining buffer ( 1× ) resuspending cells, 600g4℃ Centrifugation 3 ～ 4 Minutes, the cells were pelleted and the supernatant was discarded. \u003cbr\u003e Re-join 1mLJC-1 Staining buffer ( 1× ) resuspending cells, 600g4℃ Centrifugation 3 ～ 4 Minutes, the cells were pelleted and the supernatant was discarded. Use an appropriate amount JC-1 Staining buffer ( 1× ) After resuspension, observe with a fluorescence microscope or laser confocal microscope, or detect with a fluorescence spectrophotometer or analyze with a flow cytometer. \u003c\/p\u003e\n\u003cp\u003e4 For adherent cells, \u003cbr\u003e Note: For adherent cells, if you want to use fluorescence spectrophotometer or flow cytometer detection, you can collect the cells first, and refer to the detection method of suspended cells after resuspension. \u003cbr\u003e For one well of a six-well plate, the culture medium is aspirated and, if necessary, can be used according to the specific experiment PBS Or other suitable solution wash that cells once and add 1mL Cell culture fluid. The cell culture medium may contain serum and phenol red. join 1mLJC-1 Dyeing working solution, mix thoroughly. In cell incubator 37℃ incubation 20 Minutes. \u003cbr\u003e During the incubation, according to each 1mLJC-1 Staining buffer ( 5× ) join 4mL Proportion of distilled water, prepare an appropriate amount JC-1 Staining buffer ( 1× ) and placed in an ice bath. \u003cbr\u003e37℃ After the incubation, the supernatant was aspirated and treated with JC-1 Staining buffer ( 1× ) washing 2 Times. \u003cbr\u003e join 2mL The cell culture medium may contain serum and phenol red. \u003cbr\u003e Observe under fluorescence microscope or laser confocal microscope. \u003c\/p\u003e\n\u003cp\u003e5 For purified mitochondria \u003cbr\u003e Put the prepared JC-1 Reuse of dyeing working solution JC-1 Staining buffer ( 1× ) dilution 5 Times. \u003cbr\u003e0.9mL5 Double diluted JC-1 Add to the dyeing working solution 0.1mL The total protein content was 10 ～ 100μg Purified mitochondria. Detection with a fluorescence spectrophotometer or a fluorescence microplate reader: After mixing, time scan with a fluorescence spectrophotometer directly ( timescan ), the excitation wavelength is 485nm , emission wavelength is 590nm 。 If a fluorescent microplate reader is used, the excitation wavelength cannot be set to 485nm When, you can 475 ～ 520nm The excitation wavelength is set within the range. In addition, you can also refer to the following steps 6 The wavelength setting in is performed for fluorescence detection. \u003cbr\u003e Observation by fluorescence microscope or laser confocal microscope: the method is the same as the following steps 6 。 \u003c\/p\u003e\n\u003cp\u003e6 , fluorescence observation and result analysis detection \u003cbr\u003eJC-1 The excitation light can be set to 490nm , the emitted light is set to 530nm ； Detection JC-1 Polymer, the excitation light may be set to 525nm , the emitted light is set to 590nm 。 Note that it is not necessary to set the excitation light and the emission light at the maximum excitation wavelength and the maximum emission wavelength when measuring fluorescence here. If observed using a fluorescence microscope, detect JC-1 When monomer, you can refer to the settings when observing other green fluorescence, such as observing GFP Or FITC Time setting; Detection JC-1 Polymer may be observed with reference to other red fluorescence such as propidium iodide or Cy3 When setting. The appearance of green fluorescence indicates that the mitochondrial membrane potential decreases, and the cell is probably in the early stage of apoptosis. The appearance of red fluorescence indicates that the mitochondrial membrane potential is relatively normal and the state of cells is also relatively normal. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv style=\"width: 100%; white-space: normal;\"\u003e    Mitochondrial membrane potential detection kit ( JC-1 ) is a method with JC-1 It is a fluorescent probe, which can quickly and sensitively detect the changes of membrane potential of cells, tissues or purified mitochondria, and can be used for early detection of apoptosis. \u003cbr\u003e   JC-1 Is a widely used method for detecting mitochondrial membrane potential △Ψm Ideal fluorescent probe. Cellular, tissue, or purified mitochondrial membrane potentials can be detected. When mitochondrial membrane potential is high, JC-1 Aggregate in the matrix of mitochondria, forming polymers that can   Producing red fluorescence; When mitochondrial membrane potential is low, JC-1 Cannot aggregate in the matrix of mitochondria, at which time JC-1 It is a monomer and can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization.  \u003cbr\u003e    The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. Pass JC-1 From red fluorescence   The transition to green fluorescence can easily detect the decrease in cell membrane potential, and can also be detected with JC-1 From red fluorescence to green   The transformation of color fluorescence is used as a detection index of early apoptosis.  \u003cbr\u003e   JC-1 The maximum excitation wavelength of the monomer is 515nm , the maximum emission wavelength is 529nm ； JC-1 The maximum of polymer   The excitation wavelength is 585nm , the maximum emission wavelength is 590nm 。 In actual observation, the conventional observation of red fluorescence and green fluorescence is used   Just setting it. \u003cbr\u003e    This kit provides CCCP As a positive control to induce a decrease in mitochondrial membrane potential.  \u003cbr\u003e    For samples in six-well plates, this kit can detect a total 100 Samples; For 12 The sample in the well, this kit can   To detect 200 Samples. \u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp class=\"15\" style=\"margin-bottom: 7.8000pt; margin-left: 21.0000pt; mso-para-margin-bottom: 0.5000gd; text-indent: -21.0000pt; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;\"\u003e Product composition :\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 65.3595%; height: 159px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49%; text-align: center;\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50.8%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e package \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49%;\"\u003e\u003cp\u003eJC-1(200×)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50.8%;\"\u003e\u003cp\u003e100ul\/ Tube, total  5  Tube \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49%;\"\u003e\u003cp\u003e Ultrapure water \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50.8%;\"\u003e\u003cp\u003e90mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49%;\"\u003e\u003cp\u003eJC-1  Staining buffer   (5×)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50.8%;\"\u003e\u003cp\u003e80mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49%;\"\u003e\u003cp\u003eCCCP(10mM)\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50.8%;\"\u003e\u003cp\u003e20ul\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cdiv align=\"center\"\u003e \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. JC-1 (200 ×) will solidify and stick to the bottom, wall or cover of the centrifuge tube at lower temperatures such as 4 °C and ice bath. It can be incubated in a water bath at 20 ~ 25 °C for a while until it is completely melted. Use later.\u003cbr\u003e2. JC-1 (200 ×) must be fully dissolved and mixed with the ultrapure water provided by the kit before JC-1 staining buffer (5 ×) can be added. Do not prepare JC-1 staining buffer (1 ×) first and then add JC-1 (200 ×), so JC-1 will be difficult to fully dissolve, which will seriously affect subsequent detection.\u003cbr\u003e3. When washing with JC-1 staining buffer (1 ×) after loading JC-1, keep the JC-1 staining buffer (1 ×) at about 4 ℃, and the washing effect at this time is better.\u003cbr\u003e4. After the JC-1 probe is loaded and washed, try to complete the follow-up detection within 30 minutes. Store in ice bath before testing.\u003cbr\u003e5. Do not prepare all JC-1 staining buffer (5 ×) into JC-1 staining buffer (1 ×). JC-1 staining buffer (5 ×) should be directly used during the use of this kit.\u003cbr\u003e6. If precipitate is found in JC-1 staining buffer (5 ×), it must be completely dissolved before it can be used. To promote dissolution, it can be heated at 37 °C.\u003cbr\u003e7. CCCP is a mitochondrial electron transport chain inhibitor, which is toxic. Please pay attention to careful protection.\u003cbr\u003e8. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore at 4 ℃, protected from light, shelf life for one year\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518130069579,"sku":"abs50016-100T","price":150.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mitochondrial-membrane-potential-assay-kit","title":"Mitochondrial Membrane Potential Detection Kit (jc-10)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Preparation of JC-10 dyeing working solution:\u003cbr\u003eThe amount of JC-10 staining working solution required for each well of the six-well plate is 1 mL, and the amount of JC-10 staining working solution for other culture vessels is similar; For the cell suspension, 0.5 mL of JC-10 staining working solution is required for every 500,000 to 1,000,000 cells. Take an appropriate amount of JC-10 (200 ×) and dilute JC-10 in the proportion of 8mL of ultrapure water per 50μL of JC-10 (200 ×). Thoroughly dissolve and mix JC-10 with vigorous shaking. Then add 2mL of JC-10 staining buffer (5 ×), and mix well to obtain JC-10 staining working solution.\u003cbr\u003e2. Setting of positive control:\u003cbr\u003eCCCP (10 mM) provided in the kit is recommended to be added to the cell culture medium at a ratio of 1: 1000, diluted to 10 μM, and treated the cells for 20 minutes. Subsequently, JC-10 was loaded according to the following method, and mitochondrial membrane potential was detected. For most cells, the membrane potential of mitochondria will usually be completely lost after 20 minutes of 10 μM CCCP treatment, and it should show green fluorescence after JC-10 staining; However, normal cells should show red fluorescence after staining with JC-10. For specific cells, the concentration and duration of CCCP may be different, and it is necessary to refer to relevant literature to decide.\u003cbr\u003e3. For suspension cells:\u003cbr\u003e(1) Take 100,000 to 600,000 cells and resuspend them in 0.5 mL cell culture medium. The cell culture medium can contain serum and phenol red.\u003cbr\u003e(2) Add 0.5 mL of JC-10 dyeing working solution, reverse it several times and mix well. Incubate in a cell incubator at 37 °C for 20 minutes.\u003cbr\u003e(3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath.\u003cbr\u003e(4) After the incubation at 37 °C, 600g was centrifuged at 4 °C for 3-4 minutes to precipitate the cells. Discard the supernatant and be careful not to aspirate the cells as much as possible.\u003cbr\u003e(5) Wash twice with JC-10 staining buffer (1 ×): add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600 g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant. Add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant.\u003cbr\u003e(6) After resuspending with an appropriate amount of JC-10 staining buffer (1 ×), observe with a fluorescence microscope or laser confocal microscope, or detect with a fluorescence spectrophotometer or analyze with a flow cytometer.\u003cbr\u003e4. For adherent cells:\u003cbr\u003eNote: For adherent cells, if you want to use fluorescence spectrophotometer or flow cytometer detection, you can collect the cells first, and refer to the detection method of suspended cells after resuspension.\u003cbr\u003e(1) For one well of a six-well plate, aspirate the culture medium, wash the cells once with PBS or other appropriate solution if necessary according to the specific experiment, and add 1 mL of cell culture medium. The cell culture medium may contain serum and phenol red.\u003cbr\u003e(2) Add 1mL of JC-10 dyeing working solution and mix well. Incubate in a cell incubator at 37 °C for 20 minutes.\u003cbr\u003e(3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath.\u003cbr\u003e(4) After the incubation at 37 °C, the supernatant was aspirated and washed twice with JC-10 staining buffer (1 ×).\u003cbr\u003e(5) 2 mL of cell culture medium is added, and the culture medium may contain serum and phenol red.\u003cbr\u003e(6) Observe under fluorescence microscope or laser confocal microscope.\u003cbr\u003e5. For purified mitochondria:\u003cbr\u003e(1) The prepared JC-10 staining working solution was diluted 5 times with JC-10 staining buffer (1 ×).\u003cbr\u003e(2) 0.9 mL of 5-fold diluted JC-10 staining working solution was added with 0.1 mL of purified mitochondria with a total protein amount of 10-100 μg.\u003cbr\u003e(3) Detection with a fluorescence spectrophotometer or a fluorescence microplate reader: After mixing, directly use a fluorescence spectrophotometer to time scan (time scan). The excitation wavelength is 485nm and the emission wavelength is 590nm. If a fluorescent microplate reader is used and the excitation wavelength cannot be set to 485 nm, the excitation wavelength can be set in the range of 475 to 520 nm. In addition, fluorescence detection can also be performed with reference to the wavelength setting in step 6 below.\u003cbr\u003e(4) Observation with fluorescence microscope or laser confocal microscope: The method is the same as step 6 below.\u003cbr\u003e6. Fluorescence observation and result analysis:\u003cbr\u003eWhen detecting JC-10 monomer, the excitation light can be set to 490nm and the emission light can be set to 530nm; When detecting the JC-10 polymer, the excitation light can be set to 525 nm and the emission light can be set to 590 nm. Note that it is not necessary to set the excitation light and the emission light at the maximum excitation wavelength and the maximum emission wavelength when measuring fluorescence here. For observation using a fluorescence microscope, when detecting JC-10 monomer, you can refer to the settings when observing other green fluorescence, such as the settings when observing GFP or FITC; When detecting JC-10 polymer, you can refer to the settings when observing other red fluorescence, such as propidium iodide or Cy3. The appearance of green fluorescence indicates that the mitochondrial membrane potential decreases, and the cell is probably in the early stage of apoptosis. The appearance of red fluorescence indicates that the mitochondrial membrane potential is relatively normal and the state of cells is also relatively normal.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003ePrecautions:\u003cbr\u003e1. JC-10 (200 ×) will solidify and stick to the bottom, wall or cover of the centrifuge tube at lower temperatures such as 4 °C and ice bath. It can be incubated in a water bath at 20 ~ 25 °C for a while until it is completely melted. After use.\u003cbr\u003e2. JC-10 (200 ×) must be fully dissolved and mixed with the ultrapure water provided by the kit before JC-10 staining buffer (5 ×) can be added. Do not prepare JC-10 staining buffer (1 ×) first and then add JC-10 (200 ×), as it will be difficult to fully dissolve JC-10, which will seriously affect subsequent detection.\u003cbr\u003e3. When washing with JC-10 staining buffer (1 ×) after loading JC-10, keep the JC-10 staining buffer (1 ×) at about 4 ℃, and the washing effect at this time is better.\u003cbr\u003e4. After the JC-10 probe is loaded and washed, try to complete the follow-up detection within 30 minutes. Store in ice bath before testing.\u003cbr\u003e5. Please do not prepare all JC-10 staining buffer (5 ×) into JC-10 staining buffer (1 ×). JC-10 staining buffer (5 ×) should be directly used during the use of this kit.\u003cbr\u003e6. If precipitate is found in JC-10 staining buffer (5 ×), it must be completely dissolved before use. To promote dissolution, it can be heated at 37 °C.\u003cbr\u003e7. CCCP is a mitochondrial electron transport chain inhibitor, which is toxic. Please pay attention to careful protection.\u003cbr\u003e8. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eMitochondrial membrane potential detection kit (JC-10) is a kit that uses JC-10 as a fluorescent probe to rapidly and sensitively detect the changes of mitochondrial membrane potential of cells, tissues or purified mitochondria. It can be used for early apoptosis detection.\u003cbr\u003eJC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ΔΨm. Cellular, tissue, or purified mitochondrial membrane potentials can be detected. When the mitochondrial membrane potential is high, JC-10 gathers in the matrix of mitochondria and forms a polymer, which can produce red fluorescence; When the mitochondrial membrane potential is low, JC-10 cannot gather in the mitochondrial matrix. At this time, JC-10 is a monomer and can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization.\u003cbr\u003eThe decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition from red fluorescence to green fluorescence of JC-10, and the transition from red fluorescence to green fluorescence of JC-10 can also be used as a detection index in the early stage of apoptosis.\u003cbr\u003eThe maximum excitation wavelength of JC-10 monomer is 515nm, and the maximum emission wavelength is 529nm; The JC-10 polymer has a maximum excitation wavelength of 585 nm and a maximum emission wavelength of 590 nm. In actual observation, conventional settings for observing red fluorescence and green fluorescence may be used.\u003cbr\u003eThis kit provides CCCP as a positive control for inducing a decrease in mitochondrial membrane potential. For samples in six-well plates, this kit can detect a total of 100 samples; For samples in 12 wells, this kit can detect a total of 200 samples.\u003cbr\u003e\u003cbr\u003eProduct Components:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 33.4482%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.8408%;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 11.6766%;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.8408%;\"\u003eJC-10 (200 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 11.6766%;\"\u003e100 μL\/tube, 5 tubes in total\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.8408%;\"\u003eUltrapure water\u003c\/td\u003e\n\u003ctd style=\"width: 11.6766%;\"\u003e90mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.8408%;\"\u003eJC-10 Staining Buffer (5 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 11.6766%;\"\u003e80mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.8408%;\"\u003eCCCP (10 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 11.6766%;\"\u003e20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDry and protected from light at-20 ℃, shelf life is 12 months. Ultrapure water and JC-10 staining buffer (5 ×) can also be stored at 4 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518130102347,"sku":"abs50017-100T","price":450.0,"currency_code":"USD","in_stock":true}]},{"product_id":"caspase-1","title":"Caspase 1 Activity Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Preparatory work\u003cbr\u003e1. After the lysate is dissolved, mix well and put on an ice bath for later use.\u003cbr\u003e2. After the detection buffer is dissolved, mix well and place it on an ice bath for later use.\u003cbr\u003e\u003cbr\u003e2. Determination of pNA standard curve\u003cbr\u003e1. Preparation of standard diluent: Prepare an appropriate amount of standard diluent according to the ratio of 0.1 ml of lysate per 0.9 ml of detection buffer.\u003cbr\u003e2. The pNA provided in the kit (10 mM) was diluted to 0, 10, 20, 50, 100 and 200 uM with a standard diluent as a standard.\u003cbr\u003e3. Take 100ul of each concentration and detect it with a microplate reader, or take an appropriate amount and detect it with a spectrophotometric detection cup with a capacity of no more than 100ul to determine A405.\u003cdiv\u003e4. A405 of each standard was subtracted from A405 of the blank control without pNA to calculate the actual absorbance due to pNA, and a standard curve of pNA concentration versus A405 was created. The pNA standard curve has a good linear relationship in the range of 0-20uM, as shown in the figure below.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240329\/3fd22d866bc94b129b0a094532b79d84.png\" alt=\"\" width=\"438\" height=\"324\"\u003eNote: The experimental data may vary due to different experimental conditions, testing instruments, etc. The data in the figure is for reference only.\u003cbr\u003e\u003cbr\u003e3. Sample collection\u003cbr\u003e1. Suspended cells: Centrifuge the control sample that did not induce apoptosis and the sample that induced apoptosis at 600g at 4 º C for 5 minutes to collect the cells, carefully aspirate the supernatant, and ensure that no cells are aspirated as much as possible, and wash once with PBS. After the supernatant was aspirated as before, the lysate was added at a ratio of 100 ul of lysate per 2 million cells, the pellet was resuspended, and lysed in an ice bath for 15 minutes. Go down to Step 4.\u003cbr\u003e2. Adherent cells: suck the cell culture medium for later use. The adherent cells were digested with trypsin and collected into a spare cell culture medium. Collect cells by centrifugation at 600 g at 4 º C for 5 minutes, carefully aspirate the supernatant while ensuring that no cells are aspirated as much as possible, and wash once with PBS. After the supernatant was aspirated as before, the lysate was added at a ratio of 100 ul of lysate per 2 million cells, the pellet was resuspended, and lysed in an ice bath for 15 minutes. Go down to Step 4.\u003cbr\u003e3. Tissue samples: Add lysate at a ratio of 100ul lysate per 3-10mg tissue, and homogenize with a glass homogenizer on an ice bath. The homogenate was then transferred to a 1.5 ml centrifuge tube and lysed in an ice bath for an additional 5 minutes.\u003cbr\u003e4. Centrifuge at 4 º C 16,000-20,000 g for 10-15 minutes.\u003cbr\u003e5. Transfer the supernatant to an ice bath precooled centrifuge tube.\u003cbr\u003e6. The enzyme activity of Caspase 1 was determined immediately or the sample was stored at − 80 ° C. At the same time, a small amount of sample can be taken to measure the protein concentration by Bradford method, and try to make the protein concentration reach 1-3mg\/ml, which is equivalent to at least 10-30ug of protein per 10ul of the sample to be tested. If there are fewer cells, the amount of cells can be appropriately increased.\u003cbr\u003e\u003cbr\u003e4. Detection of Caspase 1 enzyme activity\u003cbr\u003e1. Take out an appropriate amount of Ac-YVAD-pNA (2mM) and place it on an ice bath for later use.\u003cbr\u003e2. Set up the reaction system as follows:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 92.0261%; height: 125px; margin: 0px auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eBlank control\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; text-align: center; height: 22px;\"\u003eSample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003eAssay buffer\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e40ul\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e40ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003eSample to be measured\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e0ul\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003eLysate\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e50ul\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e0ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003eAc-YVAD-pNA (2 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e10ul\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 22px;\"\u003e10ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 15px;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 15px;\"\u003e100ul\u003c\/td\u003e\n\u003ctd style=\"width: 31.491%; height: 15px;\"\u003e100ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003eNote: When setting the reaction system, add the detection buffer first, then add the sample to be tested, and mix properly. Be careful to avoid bubbles during mixing. An additional 10 ul of Ac-YVAD-pNA (2 mM) was then added.\u003cbr\u003e\u003cbr\u003e3. Add Ac-YVAD-pNA (2mM) and mix well, taking care to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight.\u003cbr\u003e4. A405 of the sample minus A405 of the blank control is the absorbance produced by pNA catalyzed by Caspase 1 in the sample. The amount of pNA catalytically produced in the sample can be calculated by comparing it with the standard curve obtained in step 2.\u003cbr\u003e5. Refer to the definition of Caspase 1 enzyme activity unit from Chemicon: One unit is the amount of enzyme that will clear 1.0 nmol of the colorimetric substrate Ac-YVAD-pNA per hour at 37 º C under saturated substrate concentrations. That is, an enzyme activity unit is defined as the amount of caspase 1 enzyme that can cleave 1 nmol of Ac-YVAD-pNA to produce 1 nmol of pNA within one hour at 37 º C when the substrate is saturated. In this way, it is possible to calculate how many enzyme activity units of Caspase 1 are contained in the sample. Note: In the detection system of this kit, the initial concentration of the substrate is 0.2 mM, at which time the substrate is saturated. For many samples, the substrate is saturated within 2 hours of incubation at 37 º C; If the activity of caspase 1 enzyme in the sample is particularly high, the sample must be properly diluted with lysate before determination.\u003cbr\u003e6. Use the Bradford method to detect the protein concentration in the sample to be tested (because the lysate contains a high concentration of DTT, it is not suitable to use the BCA method for protein concentration determination). In this way, the enzyme activity unit of Caspase 1 contained per unit weight of protein in a sample can be calculated.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003einterkeukin 1β conberting enzyme,ICE,caspase-1,caspase 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMammalian tissues, cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eCaspase 1 Activity Detection Kit is a kit for detecting Caspase 1 enzyme activity or purified Caspase 1 enzyme activity in cell or tissue lysates by spectrophotometry. Caspase is a family of proteases involved in the process of apoptosis and contains more than 10 members. Among them, Caspase-1 is the only Caspase that can cleave IL-1b and IL-18 precursors to produce active cytokines. Caspase-11 cleaves the 45 kD Caspase-1 precursor protein to produce 20 kD and 10 kD fragments, which can form heterodimers and further tetramers. This tetramer has protease activity. Caspase-1 regulates apoptosis by cleaving Bcl-XL, and regulates related cytokine-mediated immune inflammatory responses by cleaving cytokine precursors.\u003cbr\u003eThis kit is based on the fact that Caspase 1 can catalyze the substrate Ac-YVAD-pNA to produce yellow pNA (p-nitroaniline), so that the activity of Caspase 1 can be detected by measuring absorbance. pNA has strong absorption near 405nm. This kit can detect 20 samples (20T) or 100 samples (100T) in addition to the standard curve when detected with a microplate reader or a spectrophotometric detection cup with a capacity not exceeding 100ul.\u003cbr\u003e\u003cstrong\u003eProduct Components:\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 62.7886%; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.4969%; text-align: center;\"\u003eProduct composition\u003c\/td\u003e\n\u003ctd style=\"width: 14.453%; text-align: center;\"\u003e20T\u003c\/td\u003e\n\u003ctd style=\"width: 11.3903%; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.4969%; text-align: center;\"\u003eLysate Lysis buffer\u003c\/td\u003e\n\u003ctd style=\"width: 14.453%; text-align: center;\"\u003e8ml\u003c\/td\u003e\n\u003ctd style=\"width: 11.3903%; text-align: center;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.4969%; text-align: center;\"\u003eAssay buffer\u003c\/td\u003e\n\u003ctd style=\"width: 14.453%; text-align: center;\"\u003e8ml\u003c\/td\u003e\n\u003ctd style=\"width: 11.3903%; text-align: center;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.4969%; text-align: center;\"\u003eAc-YVAD-pNA (2 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 14.453%; text-align: center;\"\u003e200ul\u003c\/td\u003e\n\u003ctd style=\"width: 11.3903%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 31.4969%; text-align: center;\"\u003epNA (10 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 14.453%; text-align: center;\"\u003e200ul\u003c\/td\u003e\n\u003ctd style=\"width: 11.3903%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWhen stored at-20 °C, Ac-YVAD-pNA and pNA need to be stored in the dark.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518130135115,"sku":"abs50023-20T","price":117.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518130167883,"sku":"abs50023-100T","price":333.0,"currency_code":"USD","in_stock":true}]},{"product_id":"caspase-3-7","title":"Caspase 3 \/ 7 Activity Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating Instructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIn the process of using PBS, special care should be taken to avoid microbial contamination of the solution. PBS may produce precipitation at low temperatures. If precipitate is found, place in a 37 °C water bath until completely dissolved before use. For your safety and health, please operate in a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cspan style=\"font-size: 14pt;\"\u003eInstructions for Use:\u003c\/span\u003e\u003cbr\u003e1. Preparation:\u003c\/strong\u003e\u003cbr\u003eA. After the lysate is dissolved, mixed well and placed on an ice bath for later use.\u003cbr\u003eb. The test buffer is dissolved and mixed well and placed on an ice bath for later use.\u003cbr\u003e\u003cstrong\u003e2. Determination of pNA standard curve:\u003c\/strong\u003e\u003cbr\u003eA. Preparation of standard diluent: Prepare an appropriate amount of standard diluent according to the ratio of 0.1 ml of lysate per 0.9 ml of detection buffer.\u003cbr\u003eb. The pNA (10 mM) supplied in the kit was diluted to 0, 10, 20, 50, 100 and 200 μM with a standard diluent as a standard.\u003cbr\u003ec. Take 100μl of each concentration and detect it with a microplate reader, or take an appropriate amount and detect it with a spectrophotometric detection cup with a capacity not exceeding 100μl to determine A405.\u003cbr\u003ed. The actual absorbance due to pNA was calculated by subtracting the A405 of the blank control without pNA from the A405 of each standard, and a standard curve of pNA concentration versus A405 was created.\u003cbr\u003e\u003cstrong\u003e3. Collection of samples:\u003c\/strong\u003e\u003cbr\u003eA. For suspended cells: Centrifuge the control sample that did not induce apoptosis and the sample that induced apoptosis, 600 g at 4 º C for 5 minutes to collect the cells, carefully aspirate the supernatant, and ensure that no cells are aspirated as much as possible, and wash once with PBS. After the supernatant was aspirated as before, the lysate was added at a ratio of 100 microliters of lysate per 2 million cells, the pellet was resuspended, and lysed in an ice bath for 15 minutes. Go down to step 3d.\u003cbr\u003eb. For adherent cells: aspirate the cell culture medium for later use. The adherent cells were digested with trypsin and collected into a spare cell culture medium. Cells were collected by centrifugation at 600 g at 4 º C for 5 minutes, the supernatant was carefully aspirated, while ensuring that no cells were aspirated as much as possible, and PBS was washed once. After the supernatant was aspirated as before, the lysate was added at a ratio of 100 microliters of lysate per 2 million cells, the pellet was resuspended, and lysed in an ice bath for 15 minutes. Go down to step 3d.\u003cbr\u003ec. For tissue samples: Lysate was added at a ratio of 100 microliters of lysate per 3-10 mg of tissue and homogenized with a glass homogenizer on an ice bath. The homogenate was then transferred to a 1.5 ml centrifuge tube and lysed in an ice bath for an additional 5 minutes.\u003cbr\u003ed. Centrifuge at 4 º C 16,000-20,000 g for 10-15 minutes.\u003cbr\u003ee. The supernatant was transferred to an ice bath precooled centrifuge tube.\u003cbr\u003ef. Determine the enzyme activity of caspase 3\/7 immediately or save the sample at-80 º C. At the same time, a small amount of sample can be taken to measure the protein concentration by Bradford method, and try to make the protein concentration reach 1-3mg\/ml, which is equivalent to at least 10-30μg of protein per 10 microliters of sample to be tested. If there are fewer cells, the amount of cells can be appropriately increased.\u003cbr\u003e\u003cstrong\u003e4. Detection of Caspase 3\/7 enzyme activity:\u003c\/strong\u003e\u003cbr\u003eA. Remove an appropriate amount of Ac-DEVD-pNA (2 mM) and place it on an ice bath for later use.\u003cbr\u003eb. The reaction system was set up as follows:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 60.9187%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003eBlank control\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003eSample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eAssay buffer\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003e40 μl\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003e40 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eSample to be measured\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003e0 μl\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003e50 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eLysate\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003e50 μl\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003e0 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eAc-DEVD-pNA (2 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003e10 μl\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003e10 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.8354%; text-align: center;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 15.7597%; text-align: center;\"\u003e100 μl\u003c\/td\u003e\n\u003ctd style=\"width: 15.9566%; text-align: center;\"\u003e100 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003eNote: When setting the reaction system, add the detection buffer first, then add the sample to be tested, and mix properly. Be careful to avoid bubbles during mixing. An additional 10 μl of Ac-DEVD-pNA (2 mM) was then added.\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003ec. Add Ac-DEVD-pNA (2 mM) and mix well, taking care to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight.\u003cbr\u003ed. A405 of the sample minus A405 of the blank control is the absorbance generated by pNA catalyzed by caspase 3\/7 in the sample. The amount of pNA catalytically produced in the sample can be calculated by comparing it with the standard curve obtained in step 1.\u003cbr\u003ee. Refer to the definition of caspase 3\/7 enzyme activity units from Chemicon: One unit is the amount of enzyme that will clear 1.0 nmol of the colorimetric substrate Ac-DEVD-pNA per hour at 37 º C under saturated substrate concentrations. That is, an enzyme activity unit is defined as the amount of caspase 3\/7 enzyme that can cleave 1 nmol of Ac-DEVD-pNA to produce 1 nmol of pNA within one hour at 37 º C when the substrate is saturated. This allows to calculate how many enzyme activity units of caspase 3\/7 are contained in the sample. Note: In the detection system of this kit, the initial concentration of the substrate is 0.2 mM, at which time the substrate is saturated. For many samples, the substrate is saturated within 2 hours of incubation at 37 º C; If the activity of caspase 3\/7 enzyme in the sample is particularly high, the sample must be properly diluted with lysate before determination.\u003cbr\u003ef. Use the Bradford method to detect the protein concentration in the sample to be tested (because the lysate contains a high concentration of DTT, it is not suitable to use the BCA method for protein concentration determination). In this way, the enzyme activity unit of caspase 3\/7 contained per unit weight of protein in a sample can be calculated.\u003cstrong\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCPP32,Yama,apopain,caspase-3,caspase 3,Activated caspase-3\/7,Cleaved caspase-3\/7,caspase-3\/7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ecaspase 3\/7 Activity Assay Kit (caspase 3\/7 Activity Assay Kit) is a kit for detecting caspase 3\/7 enzyme activity or purified caspase 3\/7 enzyme activity in cell or tissue lysates by spectrophotometry.\u003cbr\u003eCaspase (Cysteine-requiring Aspartate Protease) is a family of proteases that play an important role in the process of apoptosis. caspase 3, also known as CPP32, Yama or apopain, sometimes written as caspase-3 or caspase 3, belongs to the CED-3 subfamily of the caspase family and is a key enzyme in the process of apoptosis. caspase 3 is the most studied caspase in mammalian cells. Both Caspase 3 and Caspase 7 can cleave the DEVD peptide sequence, so this kit can detect caspase3\/7.\u003cbr\u003eThis kit is based on the fact that caspase 3\/7 can catalyze the substrate Ac-DEVD-pNA to produce yellow pNA (p-nitroaniline), so that the activity of caspase 3\/7 can be detected by measuring absorbance. pNA has strong absorption near 405nm.\u003cbr\u003eThis kit can detect 20 samples (20T) or 100 samples (100T) in addition to the standard curve when detected with a microplate reader or a spectrophotometric detection cup with a capacity not exceeding 100μl.\u003cbr\u003e\u003cbr\u003eProduct Components:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 58.4211%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.5689%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 8.49085%; text-align: center;\"\u003e20T\u003c\/td\u003e\n\u003ctd style=\"width: 8.45254%; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.5689%; text-align: center;\"\u003eLysate Lysis buffer\u003c\/td\u003e\n\u003ctd style=\"width: 8.49085%; text-align: center;\"\u003e8ml\u003c\/td\u003e\n\u003ctd style=\"width: 8.45254%; text-align: center;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.5689%; text-align: center;\"\u003eAssay buffer\u003c\/td\u003e\n\u003ctd style=\"width: 8.49085%; text-align: center;\"\u003e8ml\u003c\/td\u003e\n\u003ctd style=\"width: 8.45254%; text-align: center;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.5689%; text-align: center;\"\u003eAc-DEVD-pNA (2 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 8.49085%; text-align: center;\"\u003e200ul\u003c\/td\u003e\n\u003ctd style=\"width: 8.45254%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.5689%; text-align: center;\"\u003epNA (10 mM)\u003c\/td\u003e\n\u003ctd style=\"width: 8.49085%; text-align: center;\"\u003e200ul\u003c\/td\u003e\n\u003ctd style=\"width: 8.45254%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWhen stored at-20 °C, Ac-DEVD-pNA and pNA need to be stored in the dark. Valid for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518131445835,"sku":"abs50025-20T","price":117.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518131478603,"sku":"abs50025-100T","price":333.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tunel-apoptosis-detection-kit","title":"Biotin TUNEL Apoptosis Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Experimental materials (self-prepared)\u003c\/strong\u003e\u003cbr\u003ePBS buffer (1 ×, pH ~ 7.4)\u003cbr\u003e0.2% Triton X-100 (formulated in PBS)\u003cbr\u003eReagents related to paraffin section processing\u003cbr\u003e4% Paraformaldehyde (PBS formulated)\u003cbr\u003eImmunohistochemical pen\u003cbr\u003e0.3% H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e(Freshly formulated in PBS)\u003cbr\u003eNeutral resin\u003cbr\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003cbr\u003e\u003cstrong\u003e2. Experimental design\u003c\/strong\u003e\u003cbr\u003eA. Positive control (optional):\u003cbr\u003ePositive control slides were prepared by DNase I treatment. DNase I can digest single-stranded or double-stranded DNA to produce monodeoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides endonucleases, artificially causing apoptosis.  \u003cbr\u003eB. Negative control (optional):\u003cbr\u003eBiotin TUNEL Reaction Buffer without TdT Enzyme was used with ddH\u003csub\u003e2\u003c\/sub\u003eO replaces TdT Enzyme.  \u003cbr\u003eC. Experimental treatment group.  \u003cbr\u003eD. Experimental control group.  \u003cbr\u003e\u003cstrong\u003e3. Experimental steps\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. Sample preparation:\u003c\/strong\u003e \u003cbr\u003e(1) For adherent cells or cell smears\u003cbr\u003ea. PBS wash once.  \u003cbr\u003eNote: If you are worried that the cells of the cell smear will not stick firmly, you can dry the sample to make the cells stick firmly.  \u003cbr\u003eb. Fixation: Add an appropriate amount of 4% paraformaldehyde (prepared with PBS) and fix at room temperature for 30 minutes. PBS wash twice.  \u003cbr\u003ec. Permeability: Add an appropriate amount of 0.2% Triton X-100 (prepared in PBS) and permeate at room temperature for 20 minutes. PBS wash twice.  \u003cbr\u003ed. Blocking: add about 100 μL of 0.3% H per well\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003eSolution (freshly prepared with PBS), allowed to fully cover the cells, blocked at room temperature and protected from light for 30 min to inactivate the endogenous catalase in the cells, and then washed twice with PBS.  \u003cbr\u003ee. Go to step 2. TUNEL reaction.  \u003cbr\u003e(2) For suspended cells or cell suspensions\u003cbr\u003eA. Cells were collected (3-5 × 106 cells), centrifuged at 1000 rpm for 5 min, and washed twice with PBS.  \u003cbr\u003eb. Fixation: Add an appropriate amount of 4% paraformaldehyde (prepared with PBS) to fully resuspend the cells, and fix at 4 ℃ for 30 minutes. Centrifuge at 2000 rpm for 5 min and wash twice with PBS.  \u003cbr\u003ec. Permeability: Add an appropriate amount of 0.2% Triton X-100 (prepared in PBS) and permeate at room temperature for 20 minutes. Centrifuge at 2000 rpm for 5 min and wash twice with PBS.  \u003cbr\u003ed. Blocking: add about 100 μL of 0.3% H per well\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003eSolution (freshly prepared with PBS), resuspended cells were gently aspirated, blocked at room temperature and protected from light for 30 minutes to inactivate endogenous catalase in cells, and then washed twice with PBS.  \u003cbr\u003ee. Go to step 2. TUNEL reaction.  \u003cbr\u003e(3) Paraffin tissue section\u003cbr\u003eA. Dewaxing and hydration: Section samples are sequentially placed into xylene I (10 min) → xylene II (10 min) → 100% ethanol I (5 min) → 100% ethanol II (5 min) → 95% ethanol (5 min) → 90% ethanol (5 min) → 80% ethanol (5 min) → 70% ethanol (5 min) → ddH\u003csub\u003e2\u003c\/sub\u003eO Rinse for 5 minutes and rinse twice.  \u003cbr\u003eNote: Xylene is toxic and volatile, please do this in a fume hood.  \u003cbr\u003eb. Use filter paper to dry the liquid around the section sample, and circle the sample outline with an immunohistochemical pen to facilitate downstream transparency and marking.  \u003cbr\u003eNote: If the outline circle of immunohistochemical strokes is found to be destroyed in subsequent experimental operations, it is necessary to make up the drawing in time.  \u003cbr\u003ec. Permeability: Dilute 2 mg\/mL Proteinase K solution with PBS to a final concentration of 20 µ g\/mL at a ratio of 1: 100, add 100 µ L dropwise to each sample so that the solution covers the entire sample area and incubate at 20-37 ℃ for 20 min.  \u003cbr\u003eNote: Proteinase K can penetrate the cell membrane and nuclear membrane, so that the staining reagent in the subsequent step can fully enter the nucleus for reaction, and improve the labeling efficiency. Too long incubation time will increase the risk of tissue sections falling off the carrier slice in subsequent washing steps, and too short incubation time may cause insufficient permeability treatment and affect labeling efficiency. In order to obtain better results, the concentration, incubation time and temperature of Proteinase K need to be optimized according to different types of tissue samples.  \u003cbr\u003ed. Sections were rinsed twice with PBS for 5 min each time.  \u003cbr\u003eNote: Proteinase K must be cleaned during this step, otherwise it will seriously interfere with the subsequent labeling reaction.  \u003cbr\u003ee. Blocking: add an appropriate amount of 0.3% H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003eThe solution (freshly prepared in PBS) was incubated at room temperature for 30 min to inactivate the endogenous catalase in the sections.  \u003cbr\u003eF. Rinse the sections twice with PBS for 5 min each time, aspirate the excess liquid with filter paper, and place the processed sample in a wet box to keep it moist.  \u003cbr\u003eg. Go to step 2. TUNEL reaction.  \u003cbr\u003e(4) Frozen tissue section\u003cbr\u003eA. Fixation: Take out the frozen sections and warm them to room temperature. Add an appropriate amount of 4% paraformaldehyde (prepared with PBS) and fix it at room temperature for 30 minutes. PBS was rinsed twice for 10 min each time.  \u003cbr\u003eNote: If you are worried that formaldehyde will not be cleaned clean, it will affect the final dyeing effect. After formaldehyde fixation is completed, an appropriate amount of 2 mg\/mL glycine can be added to wash for 10 minutes to neutralize the residual fixative, and then wash with PBS.  \u003cbr\u003eb. Use filter paper to dry the liquid around the section sample, and circle the sample outline with an immunohistochemical pen to facilitate downstream transparency and marking.  \u003cbr\u003eNote: If the outline circle of immunohistochemical strokes is found to be destroyed in subsequent experimental operations, it is necessary to make up the drawing in time.  \u003cbr\u003ec. Permeability: Dilute 2 mg\/mL Proteinase K solution with PBS to a final concentration of 20 µ g\/mL at a ratio of 1: 100, add 100 µ L dropwise to each sample so that the solution covers the entire sample area and incubate at 20-37 ℃ for 20 min.  \u003cbr\u003eNote: Proteinase K can penetrate the cell membrane and nuclear membrane, so that the staining reagent in the subsequent step can fully enter the nucleus for reaction, and improve the labeling efficiency. Too long incubation time will increase the risk of tissue sections falling off the carrier slice in subsequent washing steps, and too short incubation time may cause insufficient permeability treatment and affect labeling efficiency. In order to obtain better results, the concentration, incubation time and temperature of Proteinase K need to be optimized according to different types of tissue samples.  \u003cbr\u003ed. Sections were rinsed twice with PBS for 5 min each time.  \u003cbr\u003eNote: Proteinase K must be cleaned during this step, otherwise it will seriously interfere with the subsequent labeling reaction.  \u003cbr\u003ee. Blocking: add an appropriate amount of 0.3% H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003eThe solution (freshly prepared in PBS) was incubated at room temperature for 30 min to inactivate the endogenous catalase in the sections.  \u003cbr\u003eF. Rinse the sections twice with PBS for 5 min each time, suck off the excess liquid with filter paper, and place the treated sections in a wet box to keep them moist.  \u003cbr\u003eg. Go to step 2. TUNEL reaction.  \u003cbr\u003e(5) Positive treatment (only positive control is subjected to this step, other samples are directly subjected to TUNEL reaction step)\u003cbr\u003eA. Using ddH at a ratio of 1: 10\u003csub\u003e2\u003c\/sub\u003e0 10 × DNase I Buffer was diluted to 1 × DNase I Buffer for later use.\u003cbr\u003eb. Add 100 µ L of 1 × DNase I Buffer dropwise to the processed sample covering the entire sample area and equilibrate at room temperature for 5 min.  \u003cbr\u003ec. DNase I (2 U\/μL) was diluted 1: 100 with 1 × DNase I Buffer to a final concentration of 20 U\/mL of the working solution.  \u003cbr\u003ed. Discard the Buffer, add 100 μL of DNase I working solution at a concentration of 20 U\/mL, and incubate at room temperature for 10 min.  \u003cbr\u003ee. Discard the DNase I working solution and wash twice with PBS.  \u003cbr\u003ef. Go to step 2. TUNEL reaction.  \u003cbr\u003e\u003cstrong\u003e2. TUNEL reaction\u003c\/strong\u003e\u003cbr\u003e(1) Prepare TUNEL reaction solution (ready for use):\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75.0329%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.707%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 16.3356%; text-align: center;\"\u003e1 sample\u003c\/td\u003e\n\u003ctd style=\"width: 15.6352%; text-align: center;\"\u003e5 samples\u003c\/td\u003e\n\u003ctd style=\"width: 15.9854%; text-align: center;\"\u003e10 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.707%; text-align: center;\"\u003eTdT enzyme\u003c\/td\u003e\n\u003ctd style=\"width: 16.3356%; text-align: center;\"\u003e1 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.6352%; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.9854%; text-align: center;\"\u003e10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.707%; text-align: center;\"\u003eBiotin TUNEL Reaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 16.3356%; text-align: center;\"\u003e49 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.6352%; text-align: center;\"\u003e2145 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.9854%; text-align: center;\"\u003e490 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.707%; text-align: center;\"\u003eTotal TUNEL reaction solution volume\u003c\/td\u003e\n\u003ctd style=\"width: 16.3356%; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.6352%; text-align: center;\"\u003e250 μL\u003c\/td\u003e\u0026lt; td style = \"width: 15.9854%; text-align: center; \"\u0026gt; 500 μL\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e(2) Add 50μL of TUNEL reaction solution to each sample so that the reaction solution evenly covers the sample. Incubate at 37 °C for 60 min.  \u003cbr\u003eNote: 50μL TUNEL reaction solution is suitable for smear, section or 96-well plate (other different well plates can appropriately adjust the volume of TUNEL reaction solution to cover cells).  \u003cbr\u003eIf the sample to be tested is a smear, section or in a 24-well plate, 12-well plate or 6-well plate, you can use an anti-evaporation film, or try to use a ziplock bag or other appropriate material to cut it into a round plastic sheet slightly smaller than the well. Add TUNEL reaction solution dropwise and cover it on the sample, which can prevent the evaporation of TUNEL reaction solution and make the TUNEL reaction solution evenly cover the sample.  \u003cbr\u003e(3) Discard the TUNEL reaction solution and wash it twice with PBS.  \u003cbr\u003e\u003cstrong\u003e3. Preparation of Streptavidin-HRP working solution and DAB color development solution\u003c\/strong\u003e\u003cbr\u003e(1) Preparation of Streptavidin-HRP working solution (ready to use):\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 69.7766%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.8099%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 17.1394%; text-align: center;\"\u003e1 sample\u003c\/td\u003e\n\u003ctd style=\"width: 17.7044%; text-align: center;\"\u003e5 samples\u003c\/td\u003e\n\u003ctd style=\"width: 16.0095%; text-align: center;\"\u003e10 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.8099%; text-align: center;\"\u003e\u003cp\u003eStreptavidin-HRP\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.1394%; text-align: center;\"\u003e1 ul\u003c\/td\u003e\n\u003ctd style=\"width: 17.7044%; text-align: center;\"\u003e5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.0095%; text-align: center;\"\u003e10 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.8099%; text-align: center;\"\u003e\u003cp\u003eStreptavidin-HRP dilution\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.1394%; text-align: center;\"\u003e49 ul\u003c\/td\u003e\n\u003ctd style=\"width: 17.7044%; text-align: center;\"\u003e245 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.0095%; text-align: center;\"\u003e490 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 41.8099%; text-align: center;\"\u003e\u003cp\u003eTotal Volume of Streptavidin-HRP Working Fluid\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.1394%; text-align: center;\"\u003e50 ul\u003c\/td\u003e\n\u003ctd style=\"width: 17.7044%; text-align: center;\"\u003e250 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.0095%; text-align: center;\"\u003e500 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e(2) Preparation of DAB chromogenic solution (ready for use):\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 69.251%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.4695%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 17.663%; text-align: center;\"\u003e1 sample\u003c\/td\u003e\n\u003ctd style=\"width: 16.1448%; text-align: center;\"\u003e5 samples\u003c\/td\u003e\n\u003ctd style=\"width: 15.3859%; text-align: center;\"\u003e10 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.4695%; text-align: center;\"\u003eDAB chromogenic solution A\u003c\/td\u003e\n\u003ctd style=\"width: 17.663%; text-align: center;\"\u003e5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.1448%; text-align: center;\"\u003e25 ul\u003c\/td\u003e\n\u003ctd style=\"width: 15.3859%; text-align: center;\"\u003e50 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.4695%; text-align: center;\"\u003eDAB chromogenic solution B\u003c\/td\u003e\n\u003ctd style=\"width: 17.663%; text-align: center;\"\u003e42.5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.1448%; text-align: center;\"\u003e212.5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 15.3859%; text-align: center;\"\u003e425 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.4695%; text-align: center;\"\u003eDAB chromogenic solution C\u003c\/td\u003e\n\u003ctd style=\"width: 17.663%; text-align: center;\"\u003e2.5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.1448%; text-align: center;\"\u003e12.5 ul\u003c\/td\u003e\n\u003ctd style=\"width: 15.3859%; text-align: center;\"\u003e25 ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.4695%; text-align: center;\"\u003eTotal volume of DAB chromogenic solution\u003c\/td\u003e\n\u003ctd style=\"width: 17.663%; text-align: center;\"\u003e50 ul\u003c\/td\u003e\n\u003ctd style=\"width: 16.1448%; text-align: center;\"\u003e250 ul\u003c\/td\u003e\n\u003ctd style=\"width: 15.3859%; text-align: center;\"\u003e500ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e4. Sample color development\u003cbr\u003e\u003c\/strong\u003e(1) Add 50 μL of Streptavidin-HRP working solution to each sample and incubate at 37 ℃ for 30 min.\u003cbr\u003eNote: 50μL Streptavidin-HRP working solution is suitable for smear, section or 96-well plate (other different well plates can appropriately adjust the volume of Streptavidin-HRP working solution to cover cells). To prevent evaporation of the Streptavidin-HRP working fluid, it is recommended to coat the samples with an anti-evaporation film.\u003cbr\u003e(2) Discard the Streptavidin-HRP working solution and wash it twice with PBS.\u003cbr\u003e(3) Add 50μL of DAB color development solution to each sample, incubate at room temperature for 5 minutes or control the staining time under a microscope according to the color development.\u003cbr\u003eNote: If the color development is very strong, the color development can be stopped in less than 5 minutes. If the color development is very weak, the color development time can be appropriately extended, or even the color development can be performed overnight.\u003cbr\u003e(4) Discard the DAB color solution and wash it twice with PBS.\u003cbr\u003e(5) (Optional) Add an appropriate amount of hematoxylin staining solution or methyl green staining solution for nucleus staining. The staining solution was discarded and washed twice with PBS.\u003cbr\u003e(6) (Optional) Slice sealing: Immerse the slices in pure water, 70% ethanol, 80% ethanol, 90% ethanol, 95% ethanol and absolute ethanol for 5 minutes in turn, and finally put the slice samples in a dyeing tank. Soak in fresh xylene, and transparent treatment twice for 5 minutes each time. After the dehydration was complete, the liquid around the sections was wiped away, 50 μL of neutral resin was added dropwise to each sample, a coverslip was covered, and the coverslip was gently tapped with the blunt end of the tweezers to remove air bubbles to complete the sealing.\u003cbr\u003e(7) Use filter paper to suck off excess liquid, add 100μL PBS to the sample area to keep the sample moist, and immediately analyze the sample under an optical microscope.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eExperiment Reagent Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003ePBS,Paraformaldehyde,Triton X-100,Methanol,H2O2,Hematoxylin (optional)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eWhen cells undergo apoptosis, some DNA endonucleases will be activated. These endonucleases will cut off the genomic DNA between nucleosomes and produce DNA fragments of 180 bp to 200 bp, which shows the specific Ladder Ladder map presented in agarose gel electrophoresis. When genomic DNA double-stranded or single-stranded breaks, a large number of sticky 3 '-OH terminals will occur, which can be combined with Biotin-dUTP under the catalysis of deoxyribonucleotide terminal transferase (TdT), so as to directly detect apoptotic cells through light microscopy. This method is called Terminal-deoxynucleotidyl transfer mediated nick end labeling (TUNEL). Since normal or proliferating cells have little DNA breakage, there is no 3 '-OH formation and little can be stained. Tunel method can stain complete single apoptotic nuclei or apoptotic bodies in situ, accurately reflect the typical biochemical and morphological characteristics of apoptosis, and can detect a very small number of apoptotic cells. Therefore, it is widely used in the research of apoptosis.  \u003cbr\u003eThe kit has a wide range of applications and can be used to detect the apoptosis of cells in frozen or paraffin sections, and can also detect the apoptosis of cultured adherent cells or suspended cells. It can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.  \u003cbr\u003e\u003cstrong\u003eComponent Information:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 73.3237%; height: 220px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e50T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eA\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eBiotin TUNEL Reaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e2 × 1. 25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eB\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eTdT Enzyme\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e50uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eC\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eStreptavidin-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e50uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eD\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eStreptavidin-HRP dilution\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e2 × 1. 25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eE\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eDAB chromogenic solution A\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e250uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eF\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eDAB chromogenic solution B\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e2 × 1. 25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eG\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eDAB chromogenic solution C\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e125uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eH\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eProteinase K (2 mg\/mL)\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e100uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eI\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; height: 22px; text-align: center;\"\u003eDNase I (2 U\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; height: 22px; text-align: center;\"\u003e13uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.30756%; text-align: center;\"\u003eJ\u003c\/td\u003e\n\u003ctd style=\"width: 35.956%; text-align: center;\"\u003e10 × DNase I Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 10.2887%; text-align: center;\"\u003e260uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. For your safety and health, please wear a lab coat and disposable gloves.\u003cbr\u003e2. Sodium azide has an inhibitory effect on HRP. Do not use reagents containing sodium azide in the experiment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product should be stored at-20 ℃ protected from light; Avoid repeated freezing and thawing. This product can be stored for up to 12 months under recommended conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"50T","offer_id":41683111215179,"sku":"abs50022-50T","price":508.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mycoplasma-stain-assay-kit","title":"Mycoplasma Stain Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Preparation of cell samples:\u003cbr\u003e(1) For adherent cells, culture the cells in a six-well plate or other multi-well plate or on a coverslip until 50%-80% full. Overfull cell culture can make it difficult to determine whether mycoplasma contamination is present or not.\u003cbr\u003e(2) For suspended cells, centrifuge to precipitate the cells, take a small amount of cells for cell smear, and fully dry them in the air.\u003cbr\u003e2. Hoechst staining solution was diluted 1:10 with PBS (1 volume of Hoechst staining solution was added to 9 volumes of PBS). The diluted Hoechst staining solution should be used within 24 hours.\u003cbr\u003e3. Add an appropriate amount of fixative to fix for 10 to 20 minutes. For one well of a six-well plate, 1 mL of fixative was added. Ensure the fixative adequately covers the sample and never wash the cells prior to fixation. For adherent cells, the culture medium needs to be removed before fixation.\u003cbr\u003e4. Remove the fixing solution and dry it in the air.\u003cbr\u003e5. Add an appropriate amount of Hoechst staining solution diluted 10 times to stain at room temperature for 10 ~ 30min. For one well of a six-well plate, 1 mL of staining solution was added. Ensure that the dyeing solution fully covers the sample, and keep it out of light when dyeing. It can be protected from light with aluminum foil paper.\u003cbr\u003e6. Remove the dyeing solution and dry it in the air.\u003cbr\u003e7. Add the anti-fluorescence quenching sealing solution provided in the kit dropwise, and observe the blue fluorescence under a fluorescence microscope after sealing. Fluorescence microscope observation requires 400 times or 1000 times magnification observation (oil mirror is required). For cell samples without mycoplasma contamination or other prokaryote contamination, only blue fluorescence of the nucleus was observed, and mitochondrial DNA was not stained.\u003cbr\u003eParticulate or filamentous blue fluorescence around the nucleus can be observed in cell samples contaminated with mycoplasma. A large amount of particulate or filamentous blue fluorescence can be observed in cells with heavy mycoplasma contamination.\u003cbr\u003eIn case of bacterial, yeast or mold contamination, although Hoechst staining will also be positive, bacterial, yeast or mold contamination can be observed under an optical microscope, but mycoplasma cannot be observed, so it can be preliminarily distinguished whether it is mycoplasma or other microorganisms.\u003cbr\u003eIf further identification is necessary, further detection can be carried out by inoculating mycoplasma culture and RT-PCR.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003esolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThe mycoplasma staining detection kit can be used for in situ staining detection of mycoplasma or other prokaryotes in cultured cells. It is mainly used to detect whether there is mycoplasma contamination in cultured cells.\u003cbr\u003eBacterial contamination, yeast contamination or mold contamination in cultured cells are all visible under the light microscope, but mycoplasma contamination is not visible under the light microscope and must be detected by specific detection methods.\u003cbr\u003eThere are many methods to detect mycoplasma contamination, including mycoplasma isolation and culture, mycoplasma-specific enzyme detection, RT-PCR detection and DNA fluorescence staining detection. The kit uses Hoechst staining to detect mycoplasma, which can quickly, effectively and highly sensitively detect mycoplasma contamination.\u003cbr\u003eIf mycoplasma contamination is found, it is recommended to replace non-contaminated cells for culture. The kit can detect at least 100 samples if used for six-well plate sample detection.\u003c\/p\u003e\n\u003cdiv\u003eComponents:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 50.9855%; height: 84px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 20.9628%; height: 21px; text-align: center;\"\u003eItem number\u003c\/td\u003e\n\u003ctd style=\"width: 47.3214%; height: 21px; text-align: center;\"\u003eProduct name\u003c\/td\u003e\n\u003ctd style=\"width: 26.3665%; height: 21px; text-align: center;\"\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 20.9628%; height: 21px; text-align: center;\"\u003eComponent A\u003c\/td\u003e\n\u003ctd style=\"width: 47.3214%; height: 21px; text-align: center;\"\u003eFixative solution\u003c\/td\u003e\n\u003ctd style=\"width: 26.3665%; height: 21px; text-align: center;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 20.9628%; height: 21px; text-align: center;\"\u003eComponent B\u003c\/td\u003e\n\u003ctd style=\"width: 47.3214%; height: 21px; text-align: center;\"\u003eHoechst\u003cspan class=\"font3\"\u003eDyeing solution\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.3665%; height: 21px; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 20.9628%; height: 21px; text-align: center;\"\u003eComponent C\u003c\/td\u003e\n\u003ctd style=\"width: 47.3214%; height: 21px; text-align: center;\"\u003eAnti-fluorescence quenching sealing solution\u003c\/td\u003e\n\u003ctd style=\"width: 26.3665%; height: 21px; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Hoechst\u003cspan class=\"font3\"\u003eThe dyeing solution is DMSO solution, which will solidify into a solid state at 4 °C. It can be melted in an oven at room temperature or 37 °C before use.\u003c\/span\u003e\u003cbr\u003e2. Hoechst dyeing reagent is harmful to human body, please pay attention to proper protection.\u003cbr\u003e3. The fixative contains glacial acetic acid and has a pungent smell. It should be fixed in the fume hood.\u003cbr\u003e4. Fluorescent dyes all have quenching problems. It is recommended to complete the test on the same day as possible after dyeing.\u003cbr\u003e5. Before detecting mycoplasma, it is best to use antibiotic-free culture medium for 2 to 3 generations, which makes it easier to detect mycoplasma, because some antibiotics can inhibit the growth of mycoplasma.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2 ~ 8 ℃ in the dark from light, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683113640011,"sku":"abs9252-100T","price":60.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-cell-detection-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}