{"title":"Cell Proliferation\/Cytotoxicity Detection Kits","description":null,"products":[{"product_id":"cell-counting-kit","title":"CCK-8 Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCCK8,CCK 8,Cell Counting Kit-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eCell Counting Kit 8, referred to as CCK-8 kit for short, is a highly sensitive, non-radioactive colorimetric detection method used to determine the number of living cells in cell proliferation or toxicity experiments.\u003c\/p\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eCCK-8 is an upgraded product of MTT. CCK-8 solution can be directly added to cell samples without pre-preparing various ingredients. It has rapid detection and very low toxicity. CCK-8 is based on the water-soluble tetrazolium salt WST-8. The working principle is: WST-8 can be reduced by dehydrogenase in mitochondria to produce orange-yellow formazan dye in the presence of electron coupling reagent. The formazan dye is able to dissolve in tissue culture medium in proportion to the number of viable cells. Through colorimetry, the number of viable cells can be dynamically quantified, so as to detect cell proliferation or drug toxicity. This kit is usually used for cycle and apoptosis detection of adherent or suspended cells. If it is used in tissue, the tissue needs to be digested into a single-cell state, and then it can be tested.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is restricted to scientific purposes only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is effective for one year when stored at 4 ℃ in the dark, and for two years when stored at-20 ℃ in the dark.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500T","offer_id":43115013406795,"sku":"abs50003-500T","price":107.0,"currency_code":"USD","in_stock":true},{"title":"5000T","offer_id":41683118751819,"sku":"abs50003-5000T","price":714.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mtt-cell-proliferation","title":"MTT Cell Proliferation and Cytotoxicity Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Place the cell plate in the sterile operating table.\u003cbr\u003e2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg\/ml MTT solution. It can be used after preparation, or stored directly at-20 ℃ in the dark from light, or stored at-20 ℃ in the dark from light after appropriately packaging as needed.]\u003cbr\u003e3. Put the cell plate back into the incubator and continue to incubate for 3-4 hours. When doing comparative experiments, the incubation time should be consistent.\u003cbr\u003e4. After incubation, take out the cell plate from the incubator and dissolve it according to the following method to produce MTT formazan crystals.\u003cbr\u003e(1) If it is an adherent cell, discard the culture medium. Add the same amount of Formazan Solvent as the initial volume. The volume of dissolved fluid may vary per well, but the total volume must be consistent for comparison.\u003cbr\u003e(2) If the removal of non-adherent cells or culture medium will cause the loss of MTT formazan, add the same amount of lysate as the initial volume directly to the culture medium.\u003cbr\u003e(3) Readings must be completed within 1 hour after addition of Formazan Solvent.\u003cbr\u003e5. Gentle shaking in a shaker can enhance dissolution. In some cases, particularly when the cell density is high, repeated pipetting is required to completely dissolve the MTT formazan crystals.\u003cbr\u003e6. Spectrophotometer to measure OD570 and reference wavelength OD690.\u003cbr\u003e7. The 96-well plate can be detected on a microplate reader equipped with a suitable filter.\u003cbr\u003e8. Other types of multi-well plates need to be spectrophotometrically detected with a cuvette or plate reader of appropriate size.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMTT Cell Proliferation and Cytotoxicity Assay Kit,MTT kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThe MTT assay, also known as MTT colorimetry, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline Formazan and deposit it in cells, while dead cells do not have this function. Within a certain range of cell numbers, the amount of MTT crystals formed is directly proportional to the number of cells. The greater the absorbance, the stronger the cell activity, indicating that the drug toxicity is less. This method has been widely used in the activity detection of some bioactive factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination. It is characterized by high sensitivity and economy.\u003c\/p\u003e\n\u003cdiv\u003eComponents: MTT (25mg), MTT solvent (5ml), Formazan solution (55ml)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Since a 96-well plate is used for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since the circle around the 96-well plate is the easiest to evaporate, you can discard the circle around the plate and add PBS, water or culture medium instead; On the other hand, a 96-well plate can be placed close to a water source in the incubator to alleviate evaporation.\u003cbr\u003e2. If the test compound used can react with MTT, centrifuge first and then discard the culture medium. Carefully flush it with PBS for 2 to 3 times, and then add the culture medium containing MTT.\u003cbr\u003e3. The production of Formazan is not only proportional to the number of living cells, but also affected by the action time. Therefore, when there are many samples, the measurement results may change with time.\u003cbr\u003e4. This product contains carcinogens, avoid contact with skin and eyes, please operate carefully. For your safety and health, please operate in a lab coat and disposable gloves.\u003cbr\u003e5. This product is only used for scientific research and cannot be used clinically or diagnostically.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2 ~ 8 ℃, protected from light, valid for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500T","offer_id":41518128595019,"sku":"abs50010-500T","price":100.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-proliferation-assay-kit","title":"MTS Cell Proliferation Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental operation:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. MTS working fluid preparation:\u003c\/strong\u003e\u003cbr\u003eMix MTS Solution and PMS Solution at a ratio of 20: 1 to obtain the MTS working solution. If the prepared MTS working solution needs to be stored, it should be stored at-20 ℃ in the dark from light. At this time, it is recommended to dispense appropriate amounts to avoid repeated freezing and thawing.\u003cbr\u003eNote: Both MTS Solution and PMS Solution are easy to decompose when exposed to light, so they need to be protected from light during preparation and use (can be wrapped in tin foil). If you want to store this kit at 4 °C for frequent use, the MTS working solution should be prepared before each test. The prepared unused MTS working solution needs to be stored at-20 °C protected from light.\u003cbr\u003e\u003cstrong\u003e2. Cell culture:\u003c\/strong\u003e\u003cbr\u003eThe cells are seeded in a suitable container such as a well plate. For 96-well plates, it is recommended to add 100uL of medium per well, and it is recommended to inoculate 5-100 × 10 per well\u003csup\u003e3\u003c\/sup\u003eCells. The cells of the experimental group can be treated with the drug of interest for a period of time according to the experimental design, at which time it is necessary to set up a blank group (containing medium only) and a control group (containing medium, cells and drug solvent).\u003cbr\u003eNote: The specific number of cells seeded per well can be adjusted according to the specific cell type.\u003cbr\u003e\u003cstrong\u003e3. MTS incubation:\u003c\/strong\u003e\u003cbr\u003e20 uL of MTS working solution was added to each well and then incubated in a cell incubator for 1-4 h.\u003cbr\u003eNote: The MTS incubation time can be adjusted for specific experiments.\u003cbr\u003e\u003cstrong\u003e4. Detection:\u003c\/strong\u003e\u003cbr\u003eThe absorbance (A) was measured at 490 nm with a microplate reader. If a 490 nm filter is not available, optionally use a 450-540 nm filter.\u003cbr\u003e\u003cstrong\u003e5. Data analysis:\u003cbr\u003e\u003c\/strong\u003eThe cell survival rate is calculated as follows\u003cbr\u003eCell viability (%) = [(A\u003csub\u003eExperimental group\u003c\/sub\u003e-A\u003csub\u003eBlank group\u003c\/sub\u003e)\/(A\u003csub\u003eControl group\u003c\/sub\u003e-A\u003csub\u003eBlank group\u003c\/sub\u003e)] ×100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMTS Cell Proliferation Assay Kit (MTS Cell Proliferation Assay Kit) is a common kit for detecting cell proliferation and toxicity. MTS is a novel tetrazolium salt, which can be reduced by succinate dehydrogenase in mitochondria of living cells to the water-soluble product Formazan. Formazan is specifically absorbed at 490 nm, so cell proliferation can be reflected by measuring the absorbance at 490 nm. The faster the cell proliferation, the higher the absorbance value.\u003cbr\u003eCompared with the common MTT kit, this kit does not need to remove the original culture medium, nor does it need to use another solvent to dissolve formazan, which is more convenient to use, and can avoid experimental errors caused by taking away part of formazan when removing the culture medium. This kit is very flexible to use, and incubation can be continued after detection to obtain better color development results. In addition, the kit has the advantages of wide linear range, high sensitivity and safe use.\u003cbr\u003eProduct Components:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 52.5641%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e500T\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e2500T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003eMTS Solution\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.1426%; text-align: center;\"\u003ePMS Solution\u003c\/td\u003e\n\u003ctd style=\"width: 12.4759%; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6838%; text-align: center;\"\u003e2.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. When the cells are cultured for a long time, the edge wells of the 96-well plate are prone to liquid evaporation. It is recommended to discard the circle around the 96-well plate and add sterile water, culture medium or PBS instead; At the same time, the 96-well plate can be placed in the incubator close to the water source.\u003cbr\u003e2. MTS Solution and PMS Solutin will solidify at low temperatures. Before use, please return to room temperature or 37 °C water bath for about 10 minutes until completely melted before use.\u003cbr\u003e3. MTS Solution and PMS Solution are sensitive to light. If stored at 4 °C, the color of the solution may change slightly. This change may increase the background absorbance value, but it will not affect the final experimental results.\u003cbr\u003e4. If it cannot be detected in time, you can add 25uL of 10% SDS to each well after the incubation in Step 3 to terminate the reaction. The SDS-treated well plate can be placed\u003cbr\u003eStore in an incubator protected from light for no more than 18 hours.\u003cbr\u003e5. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore the kit at -20°C away from light and moisture, stable for 1 year. For frequent use, the kit can be stored at 4°C away from light and moisture, stable for 6 weeks.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1000T","offer_id":41518128627787,"sku":"abs50011-1000T","price":357.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":41683113115723,"sku":"abs50011-500T","price":164.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":41683113148491,"sku":"abs50011-2500T","price":598.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/kits-cell-detection-kits-cell-proliferationcytotoxicity-detection-kits.oembed","provider":"AntBio","version":"1.0","type":"link"}