{"title":"IHC Detection Kit","description":null,"products":[{"product_id":"ihc-doublestain-kit","title":"IHC DoubleStain Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIHC DoubleStain Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePrinciple: the primary antibody recognizes the antigen, HRP-labeled secondary antibody recognizes the primary antibody, HRP catalytic intermediate TY permanently marks the antigen and realizes signal amplification, red chromogens specifically recognize the TY product (covalent binding), so as to realize the accumulation of red signal and complete the red color. DAB was used for routine immunohistochemical staining to form a brown substance. DAB is brown and red, forming a unique histochemical double staining color. The histochemical double staining signal can be neutrally sealed, which is different from the traditional red signal such as alkaline phosphatase AEC or AP-red (which cannot neutrally seal the slides). \u003cbr\u003e\u003cbr\u003eComponents: \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 58.801%; height: 257px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 37px;\"\u003e\n\u003ctd style=\"width: 62.9848%; text-align: center; height: 37px;\" colspan=\"2\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 37px;\"\u003eSpecification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 110px;\" rowspan=\"5\"\u003eRed chromogenic reagent\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eTY\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e8mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003ePB\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e8mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eAC\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e4mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eCU\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e600uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eRed\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 44px;\" rowspan=\"2\"\u003eDAB chromogenic solution\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eDAB chromogen（100×）\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e110uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003eDAB buffer\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e6mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 62.9848%; text-align: center; height: 22px;\" colspan=\"2\"\u003ePolymeric HRP secondary antibody\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e11mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 62.9848%; text-align: center; height: 22px;\" colspan=\"2\"\u003eAntibody diluent\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e11mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 62.9848%; text-align: center; height: 22px;\" colspan=\"2\"\u003e3% hydrogen peroxide\u003c\/td\u003e\n\u003ctd style=\"width: 31.4944%; text-align: center; height: 22px;\"\u003e11mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e(1) The color of DAB should not be too dark, too dark will affect the result of red color. \u003cbr\u003e2. The two antigens of double staining should be expressed in different positions (such as labeling different cells or different expression positions), which is not suitable for the co-localization of two antigens (different from the co-localization of fluorescence). \u003cbr\u003e3. The concentration of the primary antibody corresponding to the red chromogenic antigen should be appropriately increased, and the high-sensitivity polyantibody should be used as the histochemical secondary antibody. \u003cbr\u003e4. The first color can be red, the second color can be brown.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eRed color method: \u003c\/strong\u003eAfter the incubation of HRP secondary antibody, the reaction was added to the immediate TY for about 20min, and then the reaction was washed three times with PBS. After that, the red chromogenic solution (PB:CU: red chromogenic :AC=860:40:1:100) was added and the reaction lasted for more than 15min (\u003cstrong\u003eNote: \u003c\/strong\u003eThe above reagents were thawed and restored to liquid state before use, mixed and centrifuged if necessary. \u003cbr\u003e\u003cstrong\u003eSelf-prepared reagents: \u003c\/strong\u003ePBS, repair solution, hematoxylin, etc. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOperation steps: \u003c\/strong\u003e\u003cbr\u003e1. Paraffin sections were deparaffinized to water: successively put the sections into xylene Ⅰ for 15min — — Xylene Ⅱ15min— — Absolute ethanol Ⅰ5min— — Absolute ethanol Ⅱ5min— — 85% alcohol for 5min— — 75% alcohol for 5min— — Wash with distilled water. \u003cbr\u003e2. Antigen repair: tissue sections were placed in a repair box filled with EDTA antigen repair buffer (PH8.0) in a microwave oven for antigen repair, with a medium fire for 8 minutes to boiling, a fire stop for 8 minutes to keep warm, and then turn to a medium and low fire for 7 minutes. Other antigen repair methods can also be used, such as: 95° C water bath for 25min or high pressure etc. After natural cooling, the slide was placed in PBS (PH7.4) and shaken on a decolorization shaker for 3 times, 5min each time. \u003cbr\u003e3 To block endogenous peroxidase: The sections were placed in 3% hydrogen peroxide solution and incubated at room temperature in the dark for 25min. The slides were placed in PBS (PH7.4) and washed 3 times with shaking on a decolorization shaker for 5min each time. \u003cbr\u003e4. BSA\/ blocking solution: After the sections were slightly dried, a circle was drawn around the tissue with a histochemical pen (to prevent antibody from flowing away), and 3%BSA was dropped into the circle to evenly cover the tissue, and the slides were blocked at room temperature for 30min. \u003cbr\u003e5. Add primary antibody: gently shake off the blocking solution, drop on the section according to the recommended proportion of the primary antibody instruction of the first index primary antibody, the section is placed flat in the wet box 4° C. Incubate overnight. (Add a small amount of water in the wet box to prevent the antibody from evaporating) \u003cbr\u003e6 Add secondary antibody: The slides were washed 3 times by shaking in PBS (PH7.4) on a decolorizing shaker for 5min each time. After the sections were slightly dried, the secondary antibody (HRP labeled) of the corresponding species of the primary antibody was added to the ring to cover the tissue, and the tissue was incubated at room temperature for 50min. \u003cbr\u003e7. DAB color development: DAB and DAB buffer were prepared as working solution at a ratio of 1:100. The slide was shaken and washed 3 times in PBS (PH7.4) on a decolorizing shaker, 5min each time. After the slides were dried slightly, DAB working solution was added into the ring, and the color development time was controlled under the microscope. The positive color was brown yellow, and the color development was terminated by washing the slides with tap water. \u003cbr\u003e8. Antigen repair: the tissue sections were placed in the repair box filled with EDTA antigen repair buffer (PH8.0) in the microwave oven for the second antigen repair. Medium heat for 8min. During this process, excessive evaporation of buffer solution should be prevented and the slices should not be dried. Other antigen repair methods can also be used, such as: 95° C water bath for 25min or high pressure etc. After natural cooling, the slide was placed in PBS (PH7.4) and shaken on a decolorization shaker for 3 times, 5min each time. (Repair solution and repair conditions are determined according to the tissue) \u003cbr\u003e9. BSA\/ blocking solution: After the section is slightly dried, circle around the tissue with a histochemical pen (reinforcement to prevent antibody from flowing away), evenly cover the tissue with 3%BSA dripping in the circle, and block for 30min at room temperature. \u003cbr\u003e10. Add the primary antibody: gently shake off the blocking solution, drop on the section with PBS according to the recommended proportion of the primary antibody instruction \u003cstrong\u003esecond index \u003c\/strong\u003eprimary antibody, the section is placed flat in a dark wet box 4° C. Incubate overnight. (Add a small amount of water in the wet box to prevent the antibody from evaporating) \u003cbr\u003e11 Add secondary antibody: The slides were washed 3 times by shaking in PBS (PH7.4) on a decolorizing shaker for 5min each time. After the sections were slightly dried, the secondary antibody (HRP labeled) of the corresponding species of the primary antibody was added to the ring to cover the tissue, and the cells were incubated at room temperature for 50min. \u003cbr\u003e12. Red color: The slides were placed in PBS (PH7.4) and washed 3 times by shaking on a decolorization shaker, 5min each time. After the slides were slightly dried, the thawed TY reaction solution was added in the loop for 20min, and then the red chromogenic solution (PB:CU: red chromogenic :AC=860:40:1:100) was added for 15min. The color development time could be controlled under the microscope. The positive color was red, and the color development was terminated by rinsing the slides with tap water. \u003cbr\u003e13. Counterstain the nuclei with hematoxylin #abs9214. \u003cbr\u003e14. Dehydration and sealing: put the sections in 75% alcohol for 6min. — 85% alcohol 6min— — Anhydrous ethanol Ⅰ6min— — Anhydrous ethanol Ⅱ6min— — Dehydrated and transparent in xylene I for 5min. Take the slices out of xylene to dry slightly and seal the slices with neutral gum. \u003cbr\u003e15. Microscopic examination, image acquisition and analysis. \u003cbr\u003e16. Interpretation of immunohistochemical results in paraffin sections: the nuclei stained with hematoxylin were blue, the positive expression of DAB in the first indicator was brown yellow, and the positive expression of red in the second indicator was red or pink.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003estored at 4°C, Red chromogenic reagent - 20 ° C, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"50T","offer_id":41293314490443,"sku":"abs9792-50T","price":625.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6caa113dc9324a08a4a81e3cee1151ce.png?v=1789743799"},{"product_id":"anti-rabbit-and-mouse-hrp-dab-ihc-detection-kit-2-step","title":"Anti-Rabbit and Mouse HRP-DAB IHC detection kit (2-step)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs, Rb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAnti-Rabbit and Mouse HRP-DAB IHC detection kit (2-step) utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml(for50slides)","offer_id":41293357580363,"sku":"S0C2011-5ml(for50slides)","price":150.0,"currency_code":"USD","in_stock":true},{"title":"50ml(for500slides)","offer_id":43070816518219,"sku":"S0C2011-50ml(for500slides)","price":920.0,"currency_code":"USD","in_stock":true},{"title":"100ml(for1000slides)","offer_id":41293357613131,"sku":"S0C2011-100ml(for1000slides)","price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d647bd743b7d4c629dad6f00532e84f8.jpg?v=1787684593"},{"product_id":"four-color-multi-label-immunofluorescence-kit","title":"Absin 4-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al. Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 99%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 88.5502%;\" colspan=\"10\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50012 (Pika) Four Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\"\u003e\u003cp\u003e\u003cstrong\u003e  Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003eTSA650\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 650 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518128857163,"sku":"abs50012-20T","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518128889931,"sku":"abs50012-100T","price":776.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518128922699,"sku":"abs50012-50T","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/634116b0a06d43679cd2a5142bd4efea.jpg?v=1789725773"},{"product_id":"five-color-multi-label-immunofluorescence-kit","title":"Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1. Formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2. Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3. Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4. Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5. The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6. The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8. The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1. \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e (1)Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e (2)Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eIII. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1.dewaxing and hydration \u003cbr\u003e(1) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e(2) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e(3) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e(4) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(5) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2. Microwave repair antigen \u003cbr\u003e(1) The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e(2) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e(3) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e(4) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3. Quenching and sealing \u003cbr\u003e(1) Remove that residual lotion from the slide. \u003cbr\u003e(2) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(3) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4. Primary antibody incubation \u003cbr\u003e(1) Remove the blocking fluid from the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7. A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e(3) Repeat steps 4-6\u003cbr\u003e(4) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9. The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅣ. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1. Quenching, transparency, sealing \u003cbr\u003e(1) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e(2) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(3) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e(4) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(5) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2. Primary antibody incubation \u003cbr\u003e(1) Remove the closure on the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e(5) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e(6) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5. A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Repeat steps 2-4 。 \u003cbr\u003e(3) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7. The stained tissue slices were observed and analyzed under a fluorescence microscope.\u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1. Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2. In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003eMultiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003eVIII. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003efixed\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eEmbedding (frozen sample)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eAntigen repair (Paraffin Section)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSodium citrate antigen retrieval solution ( 50× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003eAntibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003eAntibody eluate ( mIHC Dedicated)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eCell permeability (Required for intracellular indicators)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eQuratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eQuenching endogenous peroxidase\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePeroxidase blocking solution ( H2O2 Method)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eclose (Serum from the same source of secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003ePrimary antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eHistochemical antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePrimary and secondary antibody diluents\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003eMulticolor kit (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eLung cancer Panel (containing primary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003ebuffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 96%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 79.3271%;\" colspan=\"11\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50013 (Pika) Five-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA700\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 700 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41938007162955,"sku":"abs50013-20T","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41938007195723,"sku":"abs50013-100T","price":1204.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107283019,"sku":"abs50013-50T","price":728.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5472455c9c1a4217a11c7b2d63eda209.jpg?v=1789725776"},{"product_id":"six-color-multi-labeled-immunofluorescence-staining-kit","title":"Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWGK Germany \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cdiv\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250820\/ef34141d61d1437aaa7ea11c1df1356c.jpg\" alt=\"\" width=\"694\" height=\"192\"\u003e\n\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 98%; height: 292px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\u003ctd style=\"width: 79.3271%; height: 54px;\" colspan=\"12\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50014 (pika) six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 76px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 76px; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 76px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 76px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 76px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e690\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 76px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 76px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 76px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 76px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 76px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 76px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 76px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e80mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5ml×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e16mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, pika universal type HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePerformance Indicator\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control). After the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative.\u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect 3 sections of the same tissue, and the staining results should be consistent. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518129840203,"sku":"abs50014-20T","price":411.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518129872971,"sku":"abs50014-100T","price":1570.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518129905739,"sku":"abs50014-50T","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f061b61c8e8949269030b311f7ece068.jpg?v=1789725777"},{"product_id":"sesven-color-multi-label-immunofluorescence-kit","title":"Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1. formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2. Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3. Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4. Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5. The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6. The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8. The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1. \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4. \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eIII. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1. dewaxing and hydration \u003cbr\u003e（ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e（ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e（ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e（ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e（ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2. Microwave repair antigen \u003cbr\u003e（ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e（ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e（ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e（ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3. Quenching and sealing \u003cbr\u003e（ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e（ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e（ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4. Primary antibody incubation \u003cbr\u003e（ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e（ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5. Secondary antibody incubation \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e（ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6. Fluorescence staining to amplify the signal \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e（ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7. A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e（ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e（ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e（ 3 ) Repeat steps 4-6\u003cbr\u003e（ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9. The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1. Quenching, transparency, sealing \u003cbr\u003e（ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e（ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e（ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e（ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e（ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2. Primary antibody incubation \u003cbr\u003e（ 1 ) Remove the closure on the slide. \u003cbr\u003e（ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3. Secondary antibody incubation \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e（ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e（ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4. Fluorescence staining to amplify the signal \u003cbr\u003e（ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e（ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e（ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e（ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e（ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e（ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5. A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e（ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e（ 2 ) Repeat steps 2-4 。 \u003cbr\u003e（ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7. The stained tissue slices were observed and analyzed under a fluorescence microscope.\u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1. Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2. In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003eMultiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003eVIII. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003efixed\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eEmbedding (frozen sample)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eAntigen repair (Paraffin Section)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSodium citrate antigen retrieval solution ( 50× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003eAntibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003eAntibody eluate ( mIHC Dedicated)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eCell permeability (Required for intracellular indicators)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eQuratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eQuenching endogenous peroxidase\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePeroxidase blocking solution ( H2O2 Method)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eclose (Serum from the same source of secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003ePrimary antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eHistochemical antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePrimary and secondary antibody diluent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003eMulticolor kit (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eLung cancer Panel (containing primary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003ebuffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 78.9156%;\" colspan=\"13\" width=\"1064\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50015 (Pika) Seven-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e480\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e690\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e100mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e10μLl\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 480 、 TSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, pika universal type HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2~8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107315787,"sku":"abs50015-20T","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521814962251,"sku":"abs50015-100T","price":2046.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41521814995019,"sku":"abs50015-50T","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ca676fb4c4454307b5f2ec6f10b7b7a0.jpg?v=1789725780"},{"product_id":"tsa480","title":"mIHC Dye (TSA480)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 480, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518141571147,"sku":"abs50075-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa520","title":"mIHC Dye (TSA520)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eI. Sample Requirements\u003cbr\u003e\u003c\/strong\u003e 1. Formalin-fixed paraffin blocks or slides; large tissue blocks or TMAs; paraffin seal must be intact.\u003cbr\u003e2. For slide samples, tissue must adhere tightly to the slide without folds; slides must be free of cracks, scratches or stains.\u003cbr\u003e3. Tissue must contain \u0026gt;1,000 cells.\u003cbr\u003e4. Only solid-tumor tissue should be embedded; necrotic tumor, fine-needle aspirates or cytospins will impair staining.\u003cbr\u003e5. Tissue must be fixed in 10 % neutral buffered formalin for 18–24 h.\u003cbr\u003e6. Cut 4-µm sections on adhesion slides; prepare slides within one week after fixation.\u003cbr\u003e7. Do NOT add any adhesives to the water bath; place tissue on the front centre of the slide. Remove water by standing the slide vertically on absorbent paper; tap off drops—never wipe.\u003cbr\u003e8. Dry slide on a 45 °C hot-plate for 30 min (air-dry ≥1 h if no hot-plate).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eII. Assay Procedure\u003cbr\u003e\u003c\/strong\u003e \u003cstrong\u003e1. Equipment required:\u003c\/strong\u003e pipettes, oven, microwave, IHC pen, retrieval jar, staining jars, timer, humid chamber, cover-slips, fume hood, wash bottle, fluorescence microscope, 100 mL \u0026amp; 1,000 mL cylinders, etc.\u003cbr\u003e\u003cstrong\u003e2. Reagents required:\u003c\/strong\u003e sterile water (abs9259), xylene, ethanol (100 %, 95 %, 70 %), 10 % neutral buffered formalin, antigen-retrieval buffer, primary antibodies, polymer HRP-secondary antibodies (abs20216, abs50213), blocking buffer, TBST, anti-fade mounting medium.\u003cbr\u003e\u003cstrong\u003e3. Reagent preparation:\u003cbr\u003e\u003c\/strong\u003e   1) Fluorophore dilution: 100× stock dyes are diluted 1:100 in amplification buffer (prepare fresh).\u003cbr\u003e\u003cstrong\u003e4. Imaging:\u003c\/strong\u003e fluorescence microscope or slide scanner; filter sets must match dye specifications below.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIII. Paraffin-Section Protocol\u003cbr\u003e\u003c\/strong\u003e 1. De-paraffinisation \u0026amp; re-hydration\u003cbr\u003ea) Fresh xylene 10 min ×3.\u003cbr\u003eb) Ethanol gradient: 100 % 5 min → 95 % 5 min → 70 % 2 min.\u003cbr\u003ec) Sterile water 1 min ×3.\u003cbr\u003ed) Optional: 10 % neutral formalin or 4 % PFA 10–30 min, then water 1 min ×3.\u003cbr\u003ee) Optional: permeabilisation 15 min, PBST 3 min ×3.\u003cbr\u003e2. Antigen retrieval (microwave)\u003cbr\u003ea) Place slides in retrieval buffer (1×) in microwave jar.\u003cbr\u003eb) High power to boil; maintain low boil 15 min (top up liquid).\u003cbr\u003ec) Cool to RT.\u003cbr\u003e3. Quench \u0026amp; block\u003cbr\u003ea) Remove liquid; circle tissue with IHC pen.\u003cbr\u003eb) 3 % H₂O₂ 10 min; PBST 3 min ×3.\u003cbr\u003ec) Blocking buffer 10–30 min RT with shaking; discard.\u003cbr\u003e4. Primary antibody\u003cbr\u003ea) Add diluted primary Ab to cover tissue.\u003cbr\u003eb) Incubate 1 h RT with shaking; optimise if needed.\u003cbr\u003ec) TBST 3 min ×2.\u003cbr\u003e5. Secondary antibody\u003cbr\u003ea) Polymer-HRP secondary (abs20216 or abs50213) 10 min RT.\u003cbr\u003eb) TBST 3 min ×2.\u003cbr\u003e6. Fluorescent TSA amplification\u003cbr\u003ea) Apply 100 µL 1× dye working solution 10 min RT with shaking.\u003cbr\u003eb) TBST 3 min ×3.\u003cbr\u003ec) Microwave retrieval; cool to RT.\u003cbr\u003ed) Sterile water 1 min → TBST 2 min.\u003cbr\u003e7. Next cycle (skip for single stain)\u003cbr\u003ea) Check signal under microscope (keep tissue wet).\u003cbr\u003eb) Block 10–30 min (no H₂O₂ quench).\u003cbr\u003ec) Repeat steps 4–6.\u003cbr\u003ed) After final cycle proceed to nuclear staining.\u003cbr\u003e8. Nuclear stain \u0026amp; mounting\u003cbr\u003ea) 1× DAPI 5 min RT.\u003cbr\u003eb) TBST 2 min ×3.\u003cbr\u003ec) Add anti-fade medium; cover-slip, avoid bubbles; seal edges with clear nail polish for long-term storage.\u003cbr\u003e9. Image \u0026amp; analyse.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Frozen-Section Protocol (requires antibody-elution buffer abs994)\u003cbr\u003e\u003c\/strong\u003e 1. Quench, permeabilise, block\u003cbr\u003ea) Equilibrate slide to RT; PBST 3 min ×3.\u003cbr\u003eb) Optional: 10 % neutral formalin or 4 % PFA 10–30 min; water 1 min ×3.\u003cbr\u003ec) Optional: permeabilisation 15 min; PBST 3 min ×3.\u003cbr\u003ed) 3 % H₂O₂ 10 min; PBST 3 min ×3.\u003cbr\u003ee) Blocking buffer 10–30 min RT; discard.\u003cbr\u003e2. Primary antibody – same as paraffin step 4.\u003cbr\u003e3. Secondary antibody – same as paraffin step 5.\u003cbr\u003e4. TSA amplification\u003cbr\u003ea) 1× dye working solution 10 min RT.\u003cbr\u003eb) TBST 3 min ×3.\u003cbr\u003ec) Antibody-elution buffer (abs994) 15–20 min 37 °C.\u003cbr\u003ed) Sterile water 1 min → TBST 2 min.\u003cbr\u003e5. Next cycle (skip for single stain)\u003cbr\u003ea) Check signal; keep wet.\u003cbr\u003eb) Block 10–30 min.\u003cbr\u003ec) Repeat steps 2–4.\u003cbr\u003e6. Nuclear stain \u0026amp; mounting – identical to paraffin step 8.\u003cbr\u003e7. Image \u0026amp; analyse.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eV. Result Interpretation\u003cbr\u003e\u003c\/strong\u003e 1. Any change in retrieval, incubation time or temperature may invalidate results.\u003cbr\u003e2. Each run must include positive tissue control and negative reagent control.\u003cbr\u003e3. If positive control fails, the entire batch is invalid.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eVI. Performance Characteristics\u003cbr\u003e\u003c\/strong\u003e 1. Accuracy: positive control tissue shows expected staining; negative control is blank.\u003cbr\u003e2. Within-batch precision: triplicate sections stained with the same kit lot must yield identical patterns.\u003cbr\u003eMultiplex fluorescent IHC demonstration video\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cbr\u003e\u003cstrong\u003eVII. Dye Spectral Data\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%;\" rowspan=\"2\" width=\"159\"\u003eDye\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%;\" colspan=\"2\" width=\"162\"\u003eWavelength (nm)\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%;\" rowspan=\"2\" width=\"183\"\u003eAnalogues\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px;\" rowspan=\"2\"\u003eDisplay Colour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003eEx\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003eEm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF594\/Opal620\/Texas-Red\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy5\/AF647\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF647\/Cy5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF680\/Cy5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003eVIII. Related Products\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto; height: 881px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003e\u003cstrong\u003eCategory\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"80\"\u003e\u003cstrong\u003eCat. No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eFixation\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4 % Paraformaldehyde (universal fixative)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eEmbedding (frozen)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eOCT Compound\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e110 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eAntigen retrieval (paraffin)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTris-EDTA retrieval buffer (10×, pH 9.0)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eCitrate retrieval buffer (50×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eAntibody elution (cells, frozen, bone)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eAntibody Elution Buffer (mIHC grade)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003ePermeabilisation\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTriton X-100\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eEndogenous peroxidase quench\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePeroxidase Block (H₂O₂ method)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTween-20\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL \/ 2.5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eBlocking (serum)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eGoat Serum\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eBSA\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003ePrimary antibodies\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eIHC-validated antibodies\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eAb diluent\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"18\" width=\"129\"\u003eMultiplex kits (TSA dyes, DAPI, mount, secondary, buffer)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (770 enhanced, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (770 enhanced, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (plus, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (plus, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (plus, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (plus, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e5-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e5-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e3-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e3-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e2-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e2-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eLung-cancer panels (include primaries)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eLung TME mIHC Panel I\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eLung TME mIHC Panel II\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"3\" width=\"129\"\u003eWash buffers\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs952\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTBST (10×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL ×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST (1×, pH 7.4)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBS (1×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003ePolymer-HRP secondary\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs20216\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eHigh-sensitivity Poly-HRP Goat anti-Rabbit IgG (H+L)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50213\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eHigh-sensitivity Poly-HRP Goat anti-Rabbit\/Mouse IgG (H+L)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 520, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518141603915,"sku":"abs50076-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa570","title":"mIHC Dye (TSA570)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 570, signal amplification reaction solution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003c\/p\u003e\n\u003cp\u003e2. This kit is restricted to professional use only.\u003c\/p\u003e\n\u003cp\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003c\/p\u003e\n\u003cp\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003c\/p\u003e\n\u003cp\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003c\/p\u003e\n\u003cp\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003c\/p\u003e\n\u003cp\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003c\/p\u003e\n\u003cp\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142226507,"sku":"abs50077-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa620","title":"mIHC Dye (TSA620)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 620, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142259275,"sku":"abs50078-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa700","title":"mIHC Dye (TSA700)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 700, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142292043,"sku":"abs50079-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa770","title":"mIHC Dye (TSA770) Two-step Method","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518143144011,"sku":"abs50080-100T","price":548.0,"currency_code":"USD","in_stock":true}]},{"product_id":"anti-infection","title":"Ready to use high-efficiency immunohistochemical secondary antibody kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Following the conventional Western blot operation, after the second antibody is incubated, during the last washing, according to the size of the membrane, mix 0.5ml of solution A and 0.5ml of solution B every 10 cm2 of the membrane, and prepare a luminescent detection working solution. 2. Use flat headed tweezers to remove the membrane, with the lower edge of the membrane gently touching the absorbent paper to remove excess liquid from the membrane. The protein side of the film should be facing upwards and placed on a clean cling film (some commercially available cling films may quench fluorescence when wrapping imprinted films, so high-quality cling films should be chosen). Transfer the prepared luminescent detection solution onto the protein film using a pipette, cover it evenly, and incubate at room temperature for 2 minutes. 3. Use flat headed tweezers to grip the protein film, gently touching the lower edge of the film with absorbent paper to remove excess liquid from the film. The protein side of the film is facing upwards and wrapped in a clean cling film. Gently push out the bubbles between them and fix them in the X-ray cassette. 4. Take an X-ray film in the darkroom and place it on the wrapped film. Close the cassette and expose it for 30 seconds to 1 minute. Immediately develop and fix, shorten or extend the exposure time of the next X-ray film based on its exposure intensity (for weak signals, the exposure time can be extended to several hours).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtocol\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. According to the routine Western blot operation, after the incubation of the secondary antibody and the last wash, according to the size of the membrane, 0.5ml solution A and 0.5ml solution B were mixed per 10 cm2 membrane to prepare the luminescence detection working solution. \u003cbr\u003e\n2 Remove the membrane with flat-tipped tweezer and gently touch the lower edge of the membrane with absorbent paper to remove excess liquid from the membrane. The protein side of the membrane is facing up and placed on clean plastic wrap (some commercial plastic wrap may quench fluorescence when wrapping the imprinted film, and high-quality plastic wrap should be selected). The prepared luminescent detection solution was transferred to the protein membrane with a pipette so that it was uniformly covered and incubated for 2 min at room temperature. \u003cbr\u003e\n3. Hold the protein membrane with a flat-headed tweezer, and the lower edge of the membrane gently touches the absorbent paper to remove excess liquid on the membrane. Membrane protein on the face, wrapped in a clean plastic wrap. Gently expel the air bubbles in between and fix them in the X-ray cassette.\n\u003cbr\u003e\n4. Take an X-ray film in the dark room and place it on the wrapped film, close the cassette, and expose it for 30 seconds to 1 minute. \"The image is developed immediately, and the exposure time of the next radiograph is shortened or extended depending on its exposure intensity (for weak signals, the exposure time can be extended to several hours).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003epackaging: \u003c\/strong\u003e5ml (can be used for 50 pieces)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eapplicable: \u003c\/strong\u003eprimary antibody is from mouse or rabbit\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003echromogenic substrate: \u003c\/strong\u003edab (benzidine)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ereagent composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" width=\"250\" cellspacing=\"1\" cellpadding=\"1\" align=\"left\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\" width=\"100\" height=\"20\"\u003e\u003cstrong\u003ecomponent number \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"200\"\u003e\u003cstrong\u003ecomponent name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"50\"\u003e\u003cstrong\u003especification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\" width=\"50\"\u003e\u003cstrong\u003equantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\" width=\"100\"\u003eA\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eHydrogen peroxide\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eB\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eSuper sealing fluid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eC\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003ePrimary antibody amplification agent\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eD\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eEnzyme labeled secondary antibody polymer\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eE\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eDab a liquid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center;\"\u003eF\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003eDAB B fluid\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e160ul\u003c\/td\u003e\n\u003ctd style=\"text align: Center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u0026amp;nbsp\u003cstrong\u003ereagents not provided but required: \u003c\/strong\u003eprimary antibody (from mouse or rabbit), PBS (phosphate buffer)\u003c\/p\u003e\n\u003cp\u003ethis kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003estorage and validity period \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8° C save. Each component can be stored for at least 18 months\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eoperation steps: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003enote: all steps are operated at room temperature. Adding reagent can be titrated directly in a drop bottle or with a pipette. One drop =30-40ul\u003c\/p\u003e\n\u003cp\u003e1  Dewaxed and hydrated tissue sections\u003c\/p\u003e\n\u003cp\u003e2  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e3  According to the special requirements of the applied primary antibody, the tissue sections were pretreated\u003c\/p\u003e\n\u003cp\u003e4  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e5  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eA solution \u003c\/span\u003e\u003c\/strong\u003ewas incubated for ten minutes to block endogenous peroxidase to reduce nonspecific background staining\u003c\/p\u003e\n\u003cp\u003e6  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e7  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eB solution \u003c\/span\u003e\u003c\/strong\u003ewas incubated for five minutes to reduce nonspecific staining. Note: this step should not exceed ten minutes; If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted\u003c\/p\u003e\n\u003cp\u003e8  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e9  Add primary antibody and incubate at room temperature or 37 ℃ for 20 min\u003c\/p\u003e\n\u003cp\u003e10  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e11  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eC solution \u003c\/span\u003e\u003c\/strong\u003eincubation for ten minutes\u003c\/p\u003e\n\u003cp\u003e12  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e13  Add 100ul \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eIncubate with D solution \u003c\/span\u003e\u003c\/strong\u003efor ten minutesNote: this liquid is sensitive to light, and pay attention to avoiding light\u003c\/p\u003e\n\u003cp\u003e14  Wash 2-3 times with PBS for 5 minutes each\u003c\/p\u003e\n\u003cp\u003e15  Prepare fresh substrate solution: add 30ul of \u003cstrong\u003e\u003cspan style=\"color: #e03e2d;\"\u003eF\u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #ff0000;\"\u003e\u003cstrong\u003e liquid \u003c\/strong\u003e\u003c\/span\u003eand 1ml of \u003cstrong\u003e\u003cspan style=\"color: #ff0000;\"\u003eE solution \u003c\/span\u003e\u003c\/strong\u003emix well to prepare a fresh substrate solution (the substrate solution can be stored for 2 weeks)\u003c\/p\u003e\n\u003cp\u003e16  Add 100ul of fresh substrate solution and incubate for five minutes\u003c\/p\u003e\n\u003cp\u003e17  Deionized water flushing\u003c\/p\u003e\n\u003cp\u003e18  Counterstain, seal\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLiquid A; liquid B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":41518202519627,"sku":"abs957-5mL","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ea3ea837c23f4f29a11218e1abb7f547.png?v=1789729234"},{"product_id":"stripping-buffer","title":"Antibody Eluent (for mIHC)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eReady-to-use, no dilution required. Antibody elution: Preheat the antibody eluate at 37 °C, add a small amount of antibody eluate dropwise to cover the sample, and place it at room temperature for 3 to 5 minutes; Discard the eluate, add a sufficient amount of eluate dropwise again to cover the sample (the amount of eluate should be slightly bulged on the film, generally 150 ~ 300μL), 37 °C for 10 ~ 40min (generally 20min is enough); PBS wash 3 times (fixative can be added to briefly fix again).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. The effect of antibody elution depends on the thickness of the section, elution temperature and time, and the type of primary antibody (elution difficulty: structural membrane protein and cytoskeletal protein \u0026gt; cytoplasmic protein \u0026gt; nuclear protein). 4-8 μm slices can usually be treated in a wet box at 37 °C for 10-40 minutes.\u003cbr\u003e2. Some antibodies, such as E-cadherin and Tuj1, bind to the antigen very strongly, and the elution temperature can be increased to 50 °C, or the elution time can be extended to 60min.\u003cbr\u003e3. If there is a problem with the elution of some antibodies, you can reduce the dilution ratio of the primary antibody, or put these antibodies in the last round of TSA labeling.\u003cbr\u003e4. Appropriate protective measures should be taken to avoid contact between the reagent and the skin and eyes. If you accidentally come into contact with sensitive areas, rinse immediately with plenty of water.\u003cbr\u003e5. The activity of reagents beyond the validity period may be reduced, so please use them within the validity period of the reagent.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2 ~ 8 ℃, the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe antibody eluate is used for paraffin sections, frozen sections, bone tissue sections, cell crawling sections and other samples, and the antibody elution during TSA staining.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"30mL","offer_id":41518207828043,"sku":"abs994-30mL","price":150.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hs-ihc-kit-mnr","title":"Ready to use immunohistochemical secondary antibody kit (anti rabbit\u0026mouse)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003epackaging: \u003c\/strong\u003e5ml (can be used for 50 pieces) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eapplicable: \u003c\/strong\u003eprimary antibody is from mouse or rabbit \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003echromogenic substrate: \u003c\/strong\u003edab (benzidine) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ereagent composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" width=\"250\" cellspacing=\"1\" cellpadding=\"1\" align=\"left\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"Text align: Center; width: 127.222px;\" width=\"200\"\u003e\u003cstrong\u003ecomponent name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\" width=\"50\"\u003e\u003cstrong\u003especification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\" width=\"50\"\u003e\u003cstrong\u003equantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eEndogenous peroxidase blocker \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHRP enzyme labeled anti mouse \u0026amp; Rabbit secondary antibody polymer \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB substrate (20×)\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e0.25ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB diluent \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHematoxylin \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u0026amp;nbsp\u003cstrong\u003ereagents not provided but required: \u003c\/strong\u003eprimary antibody (from mouse or rabbit), 5% goat serum (abs933), pbst\u003c\/p\u003e\n\u003cp\u003ethis kit is the latest generation of non biotin detection system of immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003estorage and validity period \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8° C save. Each component can be stored for at least 12 months\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eoperation steps: \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003enote: all the steps are operated at room temperature. The reagent can be directly titrated in a drop bottle or with a pipette. One drop =30-40ul\u003cbr\u003e\u003cbr\u003e1Dewaxed and hydrated tissue sections \u003cbr\u003e\u003cbr\u003e2Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e3According to the special requirements of the applied primary antibody, the tissue sections were pretreated (antigen retrieval) \u003cbr\u003e\u003cbr\u003e4Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e5Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eendogenous peroxidase blocking agent \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 10 min to block endogenous peroxidase to reduce nonspecific background staining \u003cbr\u003e\u003cbr\u003e6Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e7Add 100ul of 5% to 10% normal goat serum and incubate for 20 minutes at room temperature. If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted \u003cbr\u003e\u003cbr\u003e8Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e9Add primary antibody and incubate at room temperature or 37 ℃ for 30 min \u003cbr\u003e\u003cbr\u003e10Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e11Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eHRP enzyme labeled anti mouse \u0026amp; Rabbit secondary antibody polymer \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 30 min   Note: this liquid is sensitive to light, and keep away from light \u003cbr\u003e\u003cbr\u003e12Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e13Use freshly prepared \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003edab chromogenic solution \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003e(\u003c\/span\u003e\u003cspan style=\"color: \\000000;\"\u003eDilute with \u003c\/span\u003e\u003cstrong\u003edab \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003eDilution \u003cstrong\u003e\u003cspan style=\"color: \\e03e2d;\"\u003eDAB substrate \u003c\/span\u003e\u003c\/strong\u003e, ready to use) \u003c\/span\u003e\u003c\/span\u003e, incubate at room temperature for 5 minutes (the time can be adjusted according to the color development), and rinse the slide with tap water \u003cbr\u003e\u003cbr\u003e14Add 100ul\u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003ehematoxylin \u003c\/strong\u003e\u003c\/span\u003e, incubate for 3 minutes (the time can be appropriately adjusted according to the color development), and rinse with tap water to return to blue \u003cbr\u003e\u003cbr\u003e15Dehydrated, transparent, sealed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":41518208090187,"sku":"abs996-5mL","price":250.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hs-ihc-kit-m","title":"Ready to use immunohistochemical secondary antibody kit (anti mouse)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003epackaging: \u003c\/strong\u003e5ml (can be used for 50 pieces) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eapplicable: \u003c\/strong\u003eprimary antibody is from mouse \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003echromogenic substrate: \u003c\/strong\u003edab (benzidine) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ereagent composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" width=\"250\" cellspacing=\"1\" cellpadding=\"1\" align=\"left\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"Text align: Center; width: 127.222px;\" width=\"200\"\u003e\u003cstrong\u003ecomponent name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\" width=\"50\"\u003e\u003cstrong\u003especification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\" width=\"50\"\u003e\u003cstrong\u003equantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eEndogenous peroxidase blocker \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHRP enzyme labeled anti mouse secondary antibody polymer \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB substrate (20×)\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e0.25ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB diluent \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHematoxylin \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u0026amp;nbsp\u003cstrong\u003ereagents not provided but required: \u003c\/strong\u003eprimary antibody (from mouse), 5% goat serum (abs933), pbst\u003c\/p\u003e\n\u003cp\u003ethis kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003estorage and validity period \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8° C save. Each component can be stored for at least 12 months\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eoperation steps: \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003enote: all the steps are operated at room temperature. The reagent can be directly titrated in a drop bottle or with a pipette. One drop =30-40ul\u003cbr\u003e\u003cbr\u003e1Dewaxed and hydrated tissue sections \u003cbr\u003e\u003cbr\u003e2Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e3According to the special requirements of the applied primary antibody, the tissue sections were pretreated (antigen retrieval) \u003cbr\u003e\u003cbr\u003e4Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e5Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eendogenous peroxidase blocking agent \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 10 min to block endogenous peroxidase to reduce nonspecific background staining \u003cbr\u003e\u003cbr\u003e6Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e7Add 100ul of 5% to 10% normal goat serum and incubate for 20 minutes at room temperature. If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted \u003cbr\u003e\u003cbr\u003e8Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e9Add primary antibody and incubate at room temperature or 37 ℃ for 30 min \u003cbr\u003e\u003cbr\u003e10Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e11Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eHRP enzyme labeled anti mouse \u0026amp; Rabbit secondary antibody polymer \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 30 min   Note: this liquid is sensitive to light, and keep away from light \u003cbr\u003e\u003cbr\u003e12Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e13Use freshly prepared \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003edab chromogenic solution \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003e(\u003c\/span\u003e\u003cspan style=\"color: \\000000;\"\u003eDilute with \u003c\/span\u003e\u003cstrong\u003edab \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003eDilution \u003cstrong\u003e\u003cspan style=\"color: \\e03e2d;\"\u003eDAB substrate \u003c\/span\u003e\u003c\/strong\u003e, ready to use) \u003c\/span\u003e\u003c\/span\u003e, incubate at room temperature for 5 minutes (the time can be adjusted according to the color development), and rinse the slide with tap water \u003cbr\u003e\u003cbr\u003e14Add 100ul\u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003ehematoxylin \u003c\/strong\u003e\u003c\/span\u003e, incubate for 3 minutes (the time can be appropriately adjusted according to the color development), and rinse with tap water to return to blue \u003cbr\u003e\u003cbr\u003e15Dehydrated, transparent, sealed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":41518208122955,"sku":"abs997-5mL","price":250.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hs-ihc-kit-r","title":"Ready to use immunohistochemical secondary antibody kit (anti rabbit)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003epackaging: \u003c\/strong\u003e5ml (can be used for 50 pieces) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eapplicable: \u003c\/strong\u003eprimary antibody is from rabbit \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003echromogenic substrate: \u003c\/strong\u003edab (benzidine) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ereagent composition: \u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" width=\"250\" cellspacing=\"1\" cellpadding=\"1\" align=\"left\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\" width=\"200\"\u003e\u003cstrong\u003ecomponent name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\" width=\"50\"\u003e\u003cstrong\u003especification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\" width=\"50\"\u003e\u003cstrong\u003equantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eEndogenous peroxidase blocker \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHRP enzyme labeled anti rabbit secondary antibody polymer \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB substrate (20×)\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e0.25ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eDAB diluent \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text align: Center; width: 127.222px;\"\u003eHematoxylin \u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 45.7407px;\"\u003e5ml\u003c\/td\u003e\n\u003ctd style=\"text align: Center; width: 35.3704px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u0026amp;nbsp\u003cstrong\u003ereagents not provided but required: \u003c\/strong\u003eprimary antibody (from rabbit), 5% goat serum (abs933), pbst\u003c\/p\u003e\n\u003cp\u003ethis kit is the latest generation of non biotin detection system for immunohistochemical secondary antibody based on polymer technology, with stronger signal and simpler operation. Compared with the traditional immunohistochemical secondary antibody kit, it has three characteristics: first, due to the uniqueness of the polymer, the polymer molecule formed by the direct combination of enzyme molecules and secondary antibody IgG molecules is highly sensitive, effectively reducing the amount of primary antibody used, and the primary antibody reagent can be diluted 2-4 times; Second, because the use of biotin is avoided, the non-specific background color development caused by biotin can be avoided, and a clearer color development and cleaner background can be obtained; Third, rapid and shorter reagent incubation time\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003estorage and validity period \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-8° C save. Each component can be stored for at least 12 months\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eoperation steps: \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003enote: all the steps are operated at room temperature. The reagent can be directly titrated in a drop bottle or with a pipette. One drop =30-40ul\u003cbr\u003e\u003cbr\u003e1Dewaxed and hydrated tissue sections \u003cbr\u003e\u003cbr\u003e2Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e3According to the special requirements of the applied primary antibody, the tissue sections were pretreated (antigen retrieval) \u003cbr\u003e\u003cbr\u003e4Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e5Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eendogenous peroxidase blocking agent \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 10 min to block endogenous peroxidase to reduce nonspecific background staining \u003cbr\u003e\u003cbr\u003e6Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e7Add 100ul of 5% to 10% normal goat serum and incubate for 20 minutes at room temperature. If the primary antibody is diluted in a buffer containing 5% to 10% normal goat serum, this step can be omitted \u003cbr\u003e\u003cbr\u003e8Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e9Add primary antibody and incubate at room temperature or 37 ℃ for 30 min \u003cbr\u003e\u003cbr\u003e10Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e11Add 100ul \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003eHRP enzyme labeled anti mouse \u0026amp; Rabbit secondary antibody polymer \u003c\/strong\u003e\u003c\/span\u003ewas incubated for 30 min   Note: this liquid is sensitive to light, and keep away from light \u003cbr\u003e\u003cbr\u003e12Wash 2-3 times in PBST for 5 minutes \u003cbr\u003e\u003cbr\u003e13Use freshly prepared \u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003edab chromogenic solution \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003e(\u003c\/span\u003e\u003cspan style=\"color: \\000000;\"\u003eDilute with \u003c\/span\u003e\u003cstrong\u003edab \u003c\/strong\u003e\u003cspan style=\"color: \\000000;\"\u003eDilution \u003cstrong\u003e\u003cspan style=\"color: \\e03e2d;\"\u003eDAB substrate \u003c\/span\u003e\u003c\/strong\u003e, ready to use) \u003c\/span\u003e\u003c\/span\u003e, incubate at room temperature for 5 minutes (the time can be adjusted according to the color development), and rinse the slide with tap water \u003cbr\u003e\u003cbr\u003e14Add 100ul\u003cspan style=\"color: \\ff0000;\"\u003e\u003cstrong\u003ehematoxylin \u003c\/strong\u003e\u003c\/span\u003e, incubate for 3 minutes (the time can be appropriately adjusted according to the color development), and rinse with tap water to return to blue \u003cbr\u003e\u003cbr\u003e15Dehydrated, transparent, sealed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":41518208155723,"sku":"abs998-5mL","price":250.0,"currency_code":"USD","in_stock":true}]},{"product_id":"anti-rabbit-and-mouse-hrpdab-ihc-detection-kit","title":"Anti-Rabbit and Mouse HRP\u0026DAB IHC detection kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eClone Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003epAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs, Rb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, 2 to 8 °C as supplied\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIHC-P\u003c\/td\u003e\n\u003ctd\u003eN\/A\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAnti-Rabbit and Mouse HRP-DAB IHC detection kit utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround.\u003cbr\u003ePrior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add OneStep polymer HRP Goat anti-Rabbit and Mouse IgG (H+L) (specific for IHC) and incubate for 15-30 minutes. Polymer HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml (for 50 slides)","offer_id":41518294859851,"sku":"S0C1001-5ml (for 50 slides)","price":170.0,"currency_code":"USD","in_stock":true},{"title":"50ml (for500 slides)","offer_id":41518294892619,"sku":"S0C1001-50ml (for500 slides)","price":1085.0,"currency_code":"USD","in_stock":true},{"title":"100ml (for1000 slides)","offer_id":41518294925387,"sku":"S0C1001-100ml (for1000 slides)","price":1585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a4d411c433be43199d80029af7ddd9c0.jpg?v=1787569336"},{"product_id":"antibody-diluent","title":"Antibody Diluent","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3 months from date of receipt \/ reconstitution, 4 °C as supplied\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eThis antibody Diluent is supplied as a working solution. It is for use in immunohistochemical assays. This diluent can improve IHC signal and background but not compatible with all antibody products.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25ml","offer_id":41518505820235,"sku":"S0D0001-25ml","price":10.0,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":41518505853003,"sku":"S0D0001-100ml","price":30.0,"currency_code":"USD","in_stock":true},{"title":"1L","offer_id":41518505885771,"sku":"S0D0001-1L","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c96f4a7b-7cc1-40fe-aa3b-dbdded2694a1_3420478c-225e-4ce8-931f-f950fb89494f.png?v=1789498887"},{"product_id":"normal-rabbit-serum","title":"Normal Rabbit Serum","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P, Blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied;\u003cbr\u003e2 to 8 °C for 1 week under working solution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is used for the blocking of non-specific antibody binding in tissue and cell staining.According to experimental requirements, use PBS (pH7.4) solution for 10 times dilution.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"10ml","offer_id":41518722089035,"sku":"S0D0003-10ml","price":10.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e48b677e-ff2a-4d40-8008-62b04c218909.png?v=1789581800"},{"product_id":"dab-reagent","title":"DAB Reagent","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at 2−8 °C and protected from light. Do not freeze. The expiration date is indicated on the vial label.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to polybenzimidazole, which forms fibers that are renowned for their chemical and thermal stability. Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml","offer_id":41677637058635,"sku":"S0C2008-5ml","price":250.0,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":41677637091403,"sku":"S0C2008-50ml","price":1670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/58301bce-100c-4720-9887-32857e16dc54_f67076c7-ad30-4602-b680-ce9ffe2362ce.jpg?v=1789592525"},{"product_id":"normal-goat-serum","title":"Normal Goat Serum","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P, ICC, IF, Blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied;\u003cbr\u003e2 to 8 °C for 1 week under working solution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is used for the blocking of non-specific antibody binding in tissue and cell staining.According to experimental requirements, use PBS (pH7.4) solution for 10 times dilution.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"10ml","offer_id":41677669335115,"sku":"S0D0002-10ml","price":20.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2d3f50b535294672ac9f6a308ad61ba6.jpg?v=1789581822"},{"product_id":"anti-rabbit-hrp-dab-ihc-detection-kit","title":"Anti-Rabbit HRP-DAB IHC detection kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRabbit IgG (H+L)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat pAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eImmunogen Affinity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIHC-P\u003c\/td\u003e\n\u003ctd\u003eReady-to-use\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAnti-Rabbit HRP-DAB IHC detection kit provides on step detection protocol, short incubation time and faster turnaround.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml (for50slides)","offer_id":41677717635147,"sku":"S0C2031-5ml (for50slides)","price":85.0,"currency_code":"USD","in_stock":true},{"title":"100ml(for1000slides)","offer_id":43084149260363,"sku":"S0C2031-100ml(for1000slides)","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b56c6a84-a82b-4adb-815b-7cf5f5e76c40_fd22b11f-237c-436d-9379-eb9ba9a86b7b.jpg?v=1787947340"},{"product_id":"multiple-fluorescence-immunohistochemical-staining-kit","title":"Absin 10-Color IHC Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWGK Germany \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements: \u003c\/strong\u003e\u003cbr\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 4μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 2. Inspection method: \u003c\/strong\u003e\u003cbr\u003e1 Required instruments and equipment: pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e2 Reagents required: sterilized deionized water, xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, HRP labeled secondary antibodies, TBST (Item No.: \u003cstrong\u003eabs952\u003c\/strong\u003e ) etc. \u003cbr\u003e3 Reagent preparation: \u003cbr\u003e （ 1 ) Dilution of fluorescent dyes: all dyes were 100× Mother liquor, using chromogenic buffer 1:100 Dilute and prepare the dye working solution (prepare as it is used); TSA470SN (nuclear dyes) Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e （ 2 ) Fix \/ Preparation of rapid removal two-in-one working solution: 50× The concentrate was diluted with double distilled water to 1X Working fluid (powder is 16g+92mL ddH\u003csub\u003e2\u003c\/sub\u003eO ） \u003cbr\u003e4 Detection equipment: fluorescence microscope or fluorescence full-film scanning equipment, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Paraffin section operation steps \u003c\/strong\u003e ： \u003cbr\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， TBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 ) The dewaxed and hydrated glass slides were placed in a repair cup, and the glass slides were repaired with \/ Quick removal 2-in-1 working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen and add dropwise 3% Hydrogen peroxide covers the sample area and incubates 10min ， TBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing liquid on the slide, add the blocking liquid dropwise, cover the sample area, moisturize and shake at room temperature 10-30min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (It is necessary to optimize and adjust for different antibodies). \u003cbr\u003e （ 4 ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 10min 。 \u003cbr\u003e （ 4 ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 100μL (Use signal amplifier to press 1:100 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) Microwave repair, room temperature naturally cooled to room temperature. \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×TBST buffer Immersion tablet 2min 。 \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 9 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Seal: Remove that residual washing liquid on the slide, drop the sealing liquid, cover the sample area, moisturize and shake at room temperature 10-30min The blocking solution was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） —  （ 3 Nucleation and sealing were performed after completion. \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×TSA470SN Working solution onto the sample, immerse the sample area, and incubate at room temperature 5min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections: \u003c\/strong\u003e\u003cbr\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen and add dropwise 3% Hydrogen peroxide covers the sample area and incubates 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing liquid on the slide, add the blocking liquid dropwise, cover the sample area, moisturize and shake at room temperature 10-30min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (It is necessary to optimize and adjust for different antibodies). \u003cbr\u003e （ 4 ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 10min 。 \u003cbr\u003e （ 4 ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Components A Dye working solution 100μL (Use signal amplifier to press 1:100 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e5 、 . A new round of dyeing (single dyeing can be directly performed on the first 9 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Seal: Remove that residual washing liquid on the slide, drop the sealing liquid, cover the sample area, moisturize and shake at room temperature 10-30min The blocking solution was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） - （ 3 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×TSA470SN Working solution onto the sample, immerse the sample area, and incubate at room temperature 5min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 、   The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 5. Cell climbing operation steps: \u003c\/strong\u003e\u003cbr\u003e1 Sample preparation: Prepare cell samples (cell climbing slides, cell smears, etc.). It is recommended to directly use chamber slides for cell culture to facilitate subsequent detection. Completion system   After preparation, use PBS Simple rinse ( 2×2min ）。 \u003cbr\u003e2 Cell fixation: with 4% Paraformaldehyde fixation at room temperature 10-20min ， PBS washing 3×5min 。 \u003cbr\u003e3 Quenching endogenous peroxidase: dropwise addition 3% H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e , incubated at room temperature 10-30min ， PBS washing 3×5min 。 \u003cbr\u003e4 Cell permeability (this step is omitted for membrane indicators): dropwise addition containing 1-0.25% Triton X-100 of PBS Solution, treated at room temperature 10min ， PBS washing 3×5min 。 \u003cbr\u003e5 Blocking: dropwise adding blocking solution and incubating at room temperature 30min  。 \u003cbr\u003e6 Primary antibody incubation: discard the blocking solution, drop-wise add the diluted primary antibody working solution, and place it in a light-proof wet box 4°C Incubate overnight or 37℃ 1-2h (A small amount of water can be added to the wet box to prevent   antibody incubation process dry sheet), PBS Rinse 3×5min 。 \u003cbr\u003e7 Secondary antibody incubation: drop addition of the species corresponding to the primary antibody HRP The tissue was covered with secondary antibody and incubated at room temperature in the dark 20-50min ， PBS Rinse 3×5min  。 \u003cbr\u003e8 Dye incubation: dropwise addition of ready-prepared 1×TSA Dye working solution (using signal amplifier 1:100 Dilution), reaction 1-10min ， PBS Rinse 3×5min 。 \u003cbr\u003e9 Antibody elution: antibody eluate 37℃ Preheat, shake off PBS After that, the eluent is dropwise added to infiltrate the entire climbing piece, and the elution time can be controlled at 15-30min (Difficulty of elution:   structural membrane proteins and cytoskeletal proteins\u0026gt; cytoplasmic proteins\u0026gt; nuclear proteins), PBS Rinse 3×5min 。 \u003cbr\u003e10 Repeating the step of performing [5→9] , until all indicator staining is completed. \u003cbr\u003e11 dropwise addition 1×TSA470SN Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min ， PBS Rinse 3×5min 。 \u003cbr\u003e12 Add antifluorescence quenching sealing agent dropwise, and seal the tablet with a coverslip to avoid air bubbles. \u003cbr\u003e\u003cbr\u003e13 , scanning imaging, data analysis. \u003cbr\u003e\u003cstrong\u003e  VI. Description of results: \u003c\/strong\u003e\u003cbr\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VII. Product performance indicators: \u003c\/strong\u003e\u003cbr\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cdiv\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eExperiment Reagent Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv\u003e1. Reagents required: DMSO （ abs9184 ), sterilized deionized water ( abs9259 ), xylene, ethanol ( 100% 、 95% 、 70% ）、 TBST （ abs952 ) or PBST （ abs9340 ), fluorescent encapsulant ( abs9236 ）。 \u003c\/div\u003e\r\n\u003cdiv\u003e2. Detection equipment: fluorescence microscope or fluorescence full film scanning equipment, PPD Excitation and emission filters suitable for the series of fluorescent dyes shall comply with the recommendations in the table below. \u003c\/div\u003e\r\n\u003ctable width=\"386\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\" align=\"left\"\u003e\r\n    \u003ccolgroup\u003e\n\u003ccol style=\"mso-width-source:userset;mso-width-alt:4224;width:99pt\" width=\"132\"\u003e  \u003ccol style=\"mso-width-source:userset;mso-width-alt:4064;\n width:95pt\" width=\"127\" span=\"2\"\u003e  \u003c\/colgroup\u003e\r\n    \u003ctbody\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd rowspan=\"2\" class=\"xl74\" style=\"height: 28.5pt; width: 99pt; text-align: center;\" width=\"132\" height=\"38\"\u003e Fluorescent dye name \u003c\/td\u003e\r\n            \u003ctd colspan=\"2\" class=\"xl74\" style=\"border-left: none; width: 190pt; text-align: center;\" width=\"254\"\u003e wavelength \u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd class=\"xl74\" style=\"height: 14.25pt; border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\" height=\"19\"\u003e motivate  Excitation\u003c\/td\u003e\r\n            \u003ctd class=\"xl74\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e launch    Emission\u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd class=\"xl75\" style=\"height: 14.25pt; border-top: none; width: 99pt; text-align: center;\" width=\"132\" height=\"19\"\u003eDAPI\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e360\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e461\u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd class=\"xl75\" style=\"height: 14.25pt; border-top: none; width: 99pt; text-align: center;\" width=\"132\" height=\"19\"\u003ePPD 520\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e490\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e520\u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd class=\"xl75\" style=\"height: 14.25pt; border-top: none; width: 99pt; text-align: center;\" width=\"132\" height=\"19\"\u003ePPD 570\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e550\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e570\u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n        \u003ctr style=\"height:14.25pt\" height=\"19\"\u003e\r\n            \u003ctd class=\"xl75\" style=\"height: 14.25pt; border-top: none; width: 99pt; text-align: center;\" width=\"132\" height=\"19\"\u003ePPD 650\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e640\u003c\/td\u003e\r\n            \u003ctd class=\"xl75\" style=\"border-top: none; border-left: none; width: 95pt; text-align: center;\" width=\"127\"\u003e660\u003c\/td\u003e\r\n        \u003c\/tr\u003e\r\n    \u003c\/tbody\u003e\r\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases.\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cbr\u003e\u003cstrong\u003eSchematic:\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20251209\/11068fc1976341569640de40cb03a5b3.jpg\" alt=\"\" width=\"720\" height=\"200\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 81.3306%; height: 375px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e\u003cstrong\u003e50T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Quantity \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e preservation \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 23px; text-align: center;\"\u003eTSA440N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 23px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 23px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 23px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA520N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA670N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA570N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA620N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA650N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA700N dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA540S dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003eTSA660S dye \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e Nuclear dye 470SN\u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃ Avoid light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e Rapid response buffer \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e45mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e45mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e Primary antibody diluent \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e50mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e50mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e Fix \/ Rapid removal 2-in-1 buffer \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e16g\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e16g\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e2 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e3% Hydrogen peroxide \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e 2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e blocking fluid \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e 2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.1593%; height: 22px; text-align: center;\"\u003e Crystal encapsulant \u003c\/td\u003e\n\u003ctd style=\"width: 10.6082%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.6431%; height: 22px; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.1154%; height: 22px; text-align: center;\"\u003e1 bottle \u003c\/td\u003e\n\u003ctd style=\"width: 18.9962%; height: 22px; text-align: center;\"\u003e 2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for the scientific research of immunohistochemical staining of tissues, paraffin sections, TMA chips, frozen sections and cell climbing slices. It can not be used for in vitro clinical diagnosis or human test.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e2-8℃, protected from light, valid for 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePerformance Indicator\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control). After the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative.\u003c\/p\u003e\n\u003cp\u003e2. Intra-batch repeatability: Take the same batch of kits and detect 3 sections of the same tissue, and the staining results should be consistent.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683107250251,"sku":"abs50018-100T","price":7540.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":42917894946891,"sku":"abs50018-50T","price":5421.0,"currency_code":"USD","in_stock":true}]},{"product_id":"four-color-multi-label-immunofluorescence-kit-abs50028","title":"Absin 4-Color IHC Kit(Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cdiv\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB chromogenic method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce activated fluorescent substrate. The activated substrate can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody is washed off by thermal repair, and then the primary antibody is replaced with another fluorescein substrate for a second round of incubation, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250819\/cc4cb40548eb4fbf92eec65a6cbbc1d3.jpg\" alt=\"\" width=\"723\" height=\"200\"\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 96%; height: 315px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\u003ctd style=\"height: 54px; width: 86.0823%;\" colspan=\"10\" width=\"838\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50028 (Rabbit) Four Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 99px;\"\u003e\n\u003ctd style=\"height: 99px; width: 8.53441%; text-align: center;\" width=\"141\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 6.5991%; text-align: center;\" width=\"82\"\u003e\u003cp\u003eTSA\u003cbr\u003e520\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 7.40555%; text-align: center;\" width=\"78\"\u003e\u003cp\u003eTSA\u003cbr\u003e570\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 7.59723%; text-align: center;\" width=\"71\"\u003e\u003cp\u003eTSA\u003cbr\u003e650\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 5.85584%; text-align: center;\" width=\"71\"\u003e\u003cp\u003eDAPI 100x\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 9.73571%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e Signal amplifier \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 12.6354%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 10.0203%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e Peroxidase blocking solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 6.92578%; text-align: center;\" width=\"68\"\u003e\u003cp\u003e Antibody eluate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 99px; width: 10.773%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e Anti-fluorescence quenching sealing agent \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"height: 54px; width: 8.53441%; text-align: center;\" width=\"141\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.5991%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.40555%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.59723%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 5.85584%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 9.73571%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 12.6354%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.0203%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.92578%; text-align: center;\" width=\"68\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.773%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"height: 54px; width: 8.53441%; text-align: center;\" width=\"141\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.5991%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.40555%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.59723%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 5.85584%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 9.73571%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 12.6354%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.0203%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.92578%; text-align: center;\" width=\"68\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.773%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"height: 54px; width: 8.53441%; text-align: center;\" width=\"141\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.5991%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.40555%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 7.59723%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 5.85584%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 9.73571%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 12.6354%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.0203%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 6.92578%; text-align: center;\" width=\"68\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 54px; width: 10.773%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 650 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107348555,"sku":"abs50028-20T","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683107381323,"sku":"abs50028-100T","price":776.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107414091,"sku":"abs50028-50T","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/19873200829c4a9e928b0f122df86b73.jpg?v=1789740023"},{"product_id":"five-color-multi-label-immunofluorescence-kit-abs50029","title":"Absin 5-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cdiv\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB chromogenic method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce activated fluorescent substrate. The activated substrate can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody is washed off by thermal repair, and then the primary antibody is replaced with another fluorescein substrate for a second round of incubation, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250819\/d605ba76942e4b0589dfc51a457ecdb6.jpg\" alt=\"\" width=\"683\" height=\"189\"\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 87.3031%;\" colspan=\"11\" width=\"909\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50029 (Rabbit) Five Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.69095%; text-align: center;\" width=\"140\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.40148%; text-align: center;\" width=\"83\"\u003e\u003cp\u003eTSA\u003cbr\u003e520\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.69598%; text-align: center;\" width=\"78\"\u003e\u003cp\u003eTSA\u003cbr\u003e570\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.71637%; text-align: center;\" width=\"71\"\u003e\u003cp\u003eTSA\u003cbr\u003e620\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81845%; text-align: center;\" width=\"71\"\u003e\u003cp\u003eTSA\u003cbr\u003e700\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51962%; text-align: center;\" width=\"71\"\u003e\u003cp\u003eDAPI 100x\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.29705%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e Signal amplifier \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 13.9361%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3604%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e Peroxidase blocking solution \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.93783%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e Antibody eluate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9289%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e Anti-fluorescence quenching sealing agent \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.69095%; text-align: center;\" width=\"140\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.40148%; text-align: center;\" width=\"83\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.69598%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.71637%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81845%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51962%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.29705%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 13.9361%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3604%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.93783%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9289%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.69095%; text-align: center;\" width=\"140\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.40148%; text-align: center;\" width=\"83\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.69598%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.71637%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81845%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51962%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.29705%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 13.9361%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3604%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.93783%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9289%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.69095%; text-align: center;\" width=\"140\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.40148%; text-align: center;\" width=\"83\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.69598%; text-align: center;\" width=\"78\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.71637%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81845%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51962%; text-align: center;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.29705%; text-align: center;\" width=\"85\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 13.9361%; text-align: center;\" width=\"86\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3604%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.93783%; text-align: center;\" width=\"69\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9289%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 700 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2~8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107446859,"sku":"abs50029-20T","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683107479627,"sku":"abs50029-100T","price":1204.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107512395,"sku":"abs50029-50T","price":728.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a78cc7614eb541ac9f7ab3b1e1e64b69.jpg?v=1789740026"},{"product_id":"six-color-multi-labeled-immunofluorescence-staining-kit-abs50030","title":"Absin 6-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250820\/6f5251dacaac4ed781d4923069b2ae4e.jpg\" alt=\"\" width=\"676\" height=\"187\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 94.9999%;\" colspan=\"12\" width=\"100%\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50030 (Rabbit) Six Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.16058%; text-align: center;\" width=\"14%\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.43151%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.54179%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.13542%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.26684%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e690\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81011%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.40553%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51344%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3672%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.2647%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.12872%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9741%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.16058%; text-align: center;\" width=\"14%\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.43151%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.54179%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.13542%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.26684%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81011%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.40553%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51344%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3672%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.2647%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.12872%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e80mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9741%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.16058%; text-align: center;\" width=\"14%\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.43151%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.54179%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.13542%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.26684%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81011%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.40553%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51344%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3672%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.2647%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.12872%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9741%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.16058%; text-align: center;\" width=\"14%\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.43151%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.54179%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.13542%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.26684%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.81011%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.40553%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.51344%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 16.3672%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.2647%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.12872%; text-align: center;\" width=\"7%\"\u003e\u003cp\u003e16mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.9741%; text-align: center;\" width=\"8%\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2~8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107545163,"sku":"abs50030-20T","price":411.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683107577931,"sku":"abs50030-100T","price":1570.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107610699,"sku":"abs50030-50T","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/186f47b96a114498b8f6db9ca03c21ee.jpg?v=1789740016"},{"product_id":"sesven-color-multi-label-immunofluorescence-kit-abs50031","title":"Absin 7-Color IHC Kit(Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 99%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 85.7702%;\" colspan=\"13\" width=\"1064\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50031 (Rabbit) Seven Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.06315%;\" width=\"140\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.30697%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e480\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.62455%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.41421%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.29453%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.42622%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e690\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18032%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.49518%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.41907%;\" width=\"65\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.5703%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.71509%;\" width=\"70\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.20781%;\" width=\"89\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.05283%;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.06315%;\" width=\"140\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.30697%;\" width=\"82\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.62455%;\" width=\"82\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.41421%;\" width=\"78\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.29453%;\" width=\"71\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.42622%;\" width=\"71\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18032%;\" width=\"71\"\u003e\u003cp\u003e50ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.49518%;\" width=\"71\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.41907%;\" width=\"65\"\u003e\u003cp\u003e60ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.5703%;\" width=\"85\"\u003e\u003cp\u003e60ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.71509%;\" width=\"70\"\u003e\u003cp\u003e20ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.20781%;\" width=\"89\"\u003e\u003cp\u003e100ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.05283%;\" width=\"84\"\u003e\u003cp\u003e5ml*2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.06315%;\" width=\"140\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.30697%;\" width=\"82\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.62455%;\" width=\"82\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.41421%;\" width=\"78\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.29453%;\" width=\"71\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.42622%;\" width=\"71\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18032%;\" width=\"71\"\u003e\u003cp\u003e25ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.49518%;\" width=\"71\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.41907%;\" width=\"65\"\u003e\u003cp\u003e30ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.5703%;\" width=\"85\"\u003e\u003cp\u003e30ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.71509%;\" width=\"70\"\u003e\u003cp\u003e10ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.20781%;\" width=\"89\"\u003e\u003cp\u003e50ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.05283%;\" width=\"84\"\u003e\u003cp\u003e5ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.06315%;\" width=\"140\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.30697%;\" width=\"82\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.62455%;\" width=\"82\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.41421%;\" width=\"78\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.29453%;\" width=\"71\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.42622%;\" width=\"71\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18032%;\" width=\"71\"\u003e\u003cp\u003e10ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.49518%;\" width=\"71\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.41907%;\" width=\"65\"\u003e\u003cp\u003e12ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.5703%;\" width=\"85\"\u003e\u003cp\u003e12ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.71509%;\" width=\"70\"\u003e\u003cp\u003e4ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.20781%;\" width=\"89\"\u003e\u003cp\u003e20ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.05283%;\" width=\"84\"\u003e\u003cp\u003e5ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 480 、 TSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2~8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107643467,"sku":"abs50031-20T","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683107676235,"sku":"abs50031-100T","price":2046.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107709003,"sku":"abs50031-50T","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a097cb1445254c849cb3ceabee0639fe.jpg?v=1790665214"},{"product_id":"multiplex-fluorescence-immunohistochemistry-staining-kit-lung-cancer-tumor-microenvironment-i","title":"Lung Cancer Tumor Microenvironment mIHC Detection Kit (I)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cbr\u003e\u003cstrong\u003eFrozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003ca href=\"https:\/\/www.absin.net\/stripping-buffer\/abs994.html\"\u003eabs994\u003c\/a\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003ePDL1 rabbit monoclonal antibody, PD1 rabbit monoclonal antibody, Ki67 rabbit monoclonal antibody, SOX2 rabbit monoclonal antibody, GST-π rabbit monoclonal antibody, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching tablet, DAPI.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683109249099,"sku":"abs50083-20T","price":998.0,"currency_code":"USD","in_stock":true}]},{"product_id":"multiplex-fluorescence-immunohistochemistry-staining-kit-lung-cancer-tumor-microenvironment-ii","title":"Lung Cancer Tumor Microenvironment mIHC Detection Kit (II)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cbr\u003e\u003cstrong\u003eFrozen sections and cell slides must be used together with the purchase of antibody eluate (item number:\u003ca href=\"https:\/\/www.absin.net\/stripping-buffer\/abs994.html\"\u003eabs994\u003c\/a\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003ePDL1 rabbit monoclonal antibody, PD1 rabbit monoclonal antibody, Ki67 rabbit monoclonal antibody, c-MET rabbit monoclonal antibody, MCM7 rabbit monoclonal antibody, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching tablet, DAPI.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683109281867,"sku":"abs50084-20T","price":998.0,"currency_code":"USD","in_stock":true}]},{"product_id":"three-color-multiplex-fluorescent-immunohistochemical-staining-kit-anti-rabbit-secondary-antibody","title":"Absin 3-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n3. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 201470 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250821\/c45dd0d105f44f8ea0872255247f16e9.jpg\" alt=\"\" width=\"723\" height=\"200\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 88px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"height: 66px; text-align: center; width: 84.0122%;\" colspan=\"9\"\u003e\u003cstrong\u003eabs50088 (Rabbit) Tricolor Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"text-align: center; width: 8.65384%;\"\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.55967%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.47955%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.797%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.18305%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.3774%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.1484%; text-align: center;\"\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.93992%; text-align: center;\"\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.8734%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.65384%; height: 22px; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003ctd style=\"width: 6.55967%; height: 22px; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 6.47955%; height: 22px; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 8.797%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 7.18305%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 17.3774%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.1484%; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 6.93992%; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.8734%; height: 22px; text-align: center;\"\u003e5mL×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683109707851,"sku":"abs50088-100T","price":617.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ae372bf725d243298328f3da5689ac8e.jpg?v=1789747209"},{"product_id":"three-color-multiplex-fluorescent-immunohistochemical-staining-kit-pika-universal-secondary-antibody","title":"Absin 3-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9.\u003cbr\u003e2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \u003cbr\u003e3. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 201470 (1): 46-58. \u003cbr\u003e4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250821\/e5b9f978c5f745b79f8b621050d365f4.jpg\" alt=\"\" width=\"723\" height=\"200\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 144px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"97%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"height: 66px; text-align: center; width: 84.3779%;\" colspan=\"9\"\u003e\u003cstrong\u003eabs50089 (Pika) Tricolor Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"text-align: center; width: 8.41346%;\"\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.18756%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.46198%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.54527%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.13467%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 15.6027%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85187%; text-align: center;\"\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.97032%; text-align: center;\"\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.21%; height: 44px; text-align: center;\"\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.41346%; height: 22px; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003ctd style=\"width: 6.18756%; height: 22px; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 6.46198%; height: 22px; text-align: center;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 9.54527%; height: 22px; text-align: center;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 8.13467%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 15.6027%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85187%; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 8.97032%; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 11.21%; height: 22px; text-align: center;\"\u003e5mL×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e TSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 Signal amplification reaction solution, anti-pika HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not thorough, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683109740619,"sku":"abs50089-100T","price":617.0,"currency_code":"USD","in_stock":true}]},{"product_id":"two-color-multiplex-fluorescent-immunohistochemistry-staining-kit","title":"Absin 2-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] EdHarlow, DavidLane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n3. Stack, E.C., et al., Multiplexedimmunohistochemistry, imaging, and quantitation: a view, withanassessmentofTyramidesignaIamplification, multispectraIimagingandmultiplexanalysis.Methods, 201470 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 4um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100ml measuring cylinder 1000ml Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( abs9259 ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, TBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003cstrong\u003e\u003cstrong\u003e ) Dilution of fluorescent dyes: \u003c\/strong\u003e\u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003cstrong\u003e ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-rabbit HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003ea ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003eb ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003ec ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003ed ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003ee ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003ea The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003eb ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003ec ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003ed ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003ea ) Remove that residual lotion from the slide. \u003cbr\u003eb ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003ec ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003ea ) Remove the blocking fluid from the slide. \u003cbr\u003eb ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003ec ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies), or 4℃ Incubate overnight. \u003cbr\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003ea ) Remove any remaining lotion from the slide. \u003cbr\u003eb ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003ec ) Moisturizing incubation at room temperature 10min 。 \u003cbr\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003ea ) Remove any remaining lotion from the slide. \u003cbr\u003eb ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003ec ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003ed ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003ee ) Microwave repair, room temperature naturally cooled to room temperature. \u003cbr\u003ef ) Sterilized water washing tablets 1 times, 1×TBST buffer Immersion tablet 2min 。 \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003ea ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003cbr\u003eb ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20-30min The blocking solution was removed. (No Quenching Step Required) \u003cbr\u003ec ) Repeat steps 4-6\u003cbr\u003ed ) Multiple rounds of staining repeat steps 7 ， a ） — c ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 5min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003c\/strong\u003e\u003cp\u003e1 Quenching, transparency, sealing \u003c\/p\u003e\n\u003cp\u003ea ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003c\/p\u003e\n\u003cp\u003eb ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003c\/p\u003e\n\u003cp\u003ec ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003c\/p\u003e\n\u003cp\u003ed ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003ee ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003c\/p\u003e\n\u003cp\u003e2 Primary antibody incubation \u003c\/p\u003e\n\u003cp\u003ea ) Remove the closure on the slide. \u003c\/p\u003e\n\u003cp\u003eb ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies), or 4℃ Incubate overnight. \u003c\/p\u003e\n\u003cp\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003e3 Secondary antibody incubation \u003c\/p\u003e\n\u003cp\u003ea ) Remove any remaining lotion from the slide. \u003c\/p\u003e\n\u003cp\u003eb ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Moisturizing incubation at room temperature 10min 。 \u003c\/p\u003e\n\u003cp\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003c\/p\u003e\n\u003cp\u003e4 Fluorescence staining to amplify the signal \u003c\/p\u003e\n\u003cp\u003ea ) Remove any remaining lotion from the slide. \u003c\/p\u003e\n\u003cp\u003eb ) drop-on slide with pipette 1× Dye working solution 50-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Room temperature moisturizing shaking incubation 10min 。 \u003c\/p\u003e\n\u003cp\u003ed ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003ee ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 20-30min 。 \u003c\/p\u003e\n\u003cp\u003ef ) Sterilized water washing tablets 1 times, 1×TBST buffer Immersion tablet 2min 。 \u003c\/p\u003e\n\u003cp\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003c\/p\u003e\n\u003cp\u003ea ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003c\/p\u003e\n\u003cp\u003eb ) Repeat steps 2-4 。 \u003c\/p\u003e\n\u003cp\u003ec ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003c\/p\u003e\n\u003cp\u003e6 Nucleation and sealing \u003c\/p\u003e\n\u003cp\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003c\/p\u003e\n\u003cp\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e V. Description of results: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003c\/p\u003e\n\u003cp\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003c\/p\u003e\n\u003cp\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e VI. Product performance indicators: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003c\/p\u003e\n\u003cp\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%;\" rowspan=\"2\" width=\"159\"\u003e Dye Name \u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%;\" colspan=\"2\" width=\"162\"\u003e wavelength \u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%;\" rowspan=\"2\" width=\"183\"\u003e Similar fluorescent dyes \u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px;\" rowspan=\"2\"\u003e Recommended Color Settings \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e Excitation wavelength \u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e Emission wavelength \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250821\/5e16e0cb21d542ab95552d6435ed7130.jpg\" alt=\"\" width=\"723\" height=\"200\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95.0238%; margin-left: auto; margin-right: auto; height: 72px;\" border=\"1\" width=\"899\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"height: 32px; width: 81.5569%; text-align: center;\" colspan=\"7\" width=\"66\"\u003e\u003cstrong\u003eabs50086 (Rabbit) Two-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 8.27009%; height: 30px;\"\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.89521%; height: 30px;\" width=\"66\"\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.88931%; height: 30px;\" width=\"66\"\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.3462%; height: 30px;\" width=\"66\"\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.5269%; height: 30px;\" width=\"75\"\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 13.1755%;\"\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.4537%; height: 30px;\" width=\"77\"\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.27009%; height: 10px;\"\u003e100T\u003c\/td\u003e\n\u003ctd style=\"width: 7.89521%; height: 10px;\" width=\"66\"\u003e50ul\u003c\/td\u003e\n\u003ctd style=\"width: 9.88931%; height: 10px;\" width=\"66\"\u003e100ul\u003c\/td\u003e\n\u003ctd style=\"width: 12.3462%; height: 10px;\"\u003e10ml\u003c\/td\u003e\n\u003ctd style=\"width: 21.5269%; height: 10px;\"\u003e10ml\u003c\/td\u003e\n\u003ctd style=\"width: 13.1755%;\"\u003e20ml\u003c\/td\u003e\n\u003ctd style=\"width: 8.4537%; height: 10px;\" width=\"77\"\u003e5ml*2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 570 Signal amplification reaction solution, anti-rabbit HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683109773387,"sku":"abs50086-100T","price":379.0,"currency_code":"USD","in_stock":true}]},{"product_id":"two-color-multiplex-fluorescent-immunohistochemical-staining-kit-pika-universal-secondary-antibody","title":"Absin 2-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n3. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 201470 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 4um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100ml measuring cylinder 1000ml Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( abs9259 ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, TBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003cstrong\u003e\u003cstrong\u003e ) Dilution of fluorescent dyes: \u003c\/strong\u003e\u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003cstrong\u003e ) Use of secondary antibodies: \u003c\/strong\u003e The kit is anti-pika HRP Label the secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003ea ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003eb ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003ec ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003ed ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003ee ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003ea The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003eb ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003ec ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003ed ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003ea ) Remove that residual lotion from the slide. \u003cbr\u003eb ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003ec ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003ea ) Remove the blocking fluid from the slide. \u003cbr\u003eb ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003ec ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies), or 4℃ Incubate overnight. \u003cbr\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003ea ) Remove any remaining lotion from the slide. \u003cbr\u003eb ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003ec ) Moisturizing incubation at room temperature 10min 。 \u003cbr\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003ea ) Remove any remaining lotion from the slide. \u003cbr\u003eb ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003ec ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003ed ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003ee ) Microwave repair, room temperature naturally cooled to room temperature. \u003cbr\u003ef ) Sterilized water washing tablets 1 times, 1×TBST buffer Immersion tablet 2min 。 \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003ea ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003cbr\u003eb ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20-30min The blocking solution was removed. (No Quenching Step Required) \u003cbr\u003ec ) Repeat steps 4-6\u003cbr\u003ed ) Multiple rounds of staining repeat steps 7 ， a ） — c ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 5min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003c\/strong\u003e\u003cp\u003e1 Quenching, transparency, sealing \u003c\/p\u003e\n\u003cp\u003ea ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003c\/p\u003e\n\u003cp\u003eb ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003c\/p\u003e\n\u003cp\u003ec ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003c\/p\u003e\n\u003cp\u003ed ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003ee ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003c\/p\u003e\n\u003cp\u003e2 Primary antibody incubation \u003c\/p\u003e\n\u003cp\u003ea ) Remove the closure on the slide. \u003c\/p\u003e\n\u003cp\u003eb ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies), or 4℃ Incubate overnight. \u003c\/p\u003e\n\u003cp\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003e3 Secondary antibody incubation \u003c\/p\u003e\n\u003cp\u003ea ) Remove any remaining lotion from the slide. \u003c\/p\u003e\n\u003cp\u003eb ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Moisturizing incubation at room temperature 10min 。 \u003c\/p\u003e\n\u003cp\u003ed ) with 1×TBST buffer Immersion slide 3min , repeat 1 Twice. \u003c\/p\u003e\n\u003cp\u003e4 Fluorescence staining to amplify the signal \u003c\/p\u003e\n\u003cp\u003ea ) Remove any remaining lotion from the slide. \u003c\/p\u003e\n\u003cp\u003eb ) drop-on slide with pipette 1× Dye working solution 50-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003c\/p\u003e\n\u003cp\u003ec ) Room temperature moisturizing shaking incubation 10min 。 \u003c\/p\u003e\n\u003cp\u003ed ） 1×TBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003c\/p\u003e\n\u003cp\u003ee ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 20-30min 。 \u003c\/p\u003e\n\u003cp\u003ef ) Sterilized water washing tablets 1 times, 1×TBST buffer Immersion tablet 2min 。 \u003c\/p\u003e\n\u003cp\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003c\/p\u003e\n\u003cp\u003ea ) After each round of staining, fluorescence microscope can be used to confirm the staining. TBST Cover the sample to prevent dry sheets. \u003c\/p\u003e\n\u003cp\u003eb ) Repeat steps 2-4 。 \u003c\/p\u003e\n\u003cp\u003ec ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003c\/p\u003e\n\u003cp\u003e6 Nucleation and sealing \u003c\/p\u003e\n\u003cp\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×TBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003c\/p\u003e\n\u003cp\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e V. Description of results: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003c\/p\u003e\n\u003cp\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003c\/p\u003e\n\u003cp\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e VI. Product performance indicators: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003c\/p\u003e\n\u003cp\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%;\" rowspan=\"2\" width=\"159\"\u003e Dye Name \u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%;\" colspan=\"2\" width=\"162\"\u003e wavelength \u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%;\" rowspan=\"2\" width=\"183\"\u003e Similar fluorescent dyes \u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px;\" rowspan=\"2\"\u003e Recommended Color Settings \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e Excitation wavelength \u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e Emission wavelength \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250821\/af5208b6c4794dffb3ebe105413a8736.jpg\" alt=\"\" width=\"723\" height=\"200\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 120px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"height: 66px; width: 80.5371%; text-align: center;\" colspan=\"7\" width=\"66\"\u003e\u003cstrong\u003eabs50087 (Pika) Two-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 8.63743%; text-align: center;\"\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.58789%; height: 44px; text-align: center;\" width=\"66\"\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.83563%; height: 44px; text-align: center;\" width=\"66\"\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3314%; height: 44px; text-align: center;\" width=\"66\"\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.4897%; height: 44px; text-align: center;\" width=\"75\"\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.2967%; text-align: center;\"\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.35835%; height: 44px; text-align: center;\" width=\"77\"\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 8.63743%; height: 10px; text-align: center;\"\u003e100T\u003c\/td\u003e\n\u003ctd style=\"width: 7.58789%; height: 10px; text-align: center;\" width=\"66\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 8.83563%; height: 10px; text-align: center;\" width=\"66\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 11.3314%; height: 10px; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.4897%; height: 10px; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 11.2967%; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.35835%; height: 10px; text-align: center;\" width=\"77\"\u003e5mL×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 570 Signal amplification reaction solution, anti-pika HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683109806155,"sku":"abs50087-100T","price":379.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mihc-dye-tsa540","title":"mIHC Dye (TSA540)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong\u003eI. Sample Requirements\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col start=\"1\"\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eSpecimen Types:\u003c\/strong\u003e Formalin-fixed paraffin-embedded (FFPE) tissue blocks or slides, bulk tissue sections, or tissue microarray (TMA). The paraffin seal must be intact without significant damage.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eSlide-mounted Samples:\u003c\/strong\u003e Tissue must adhere firmly to the slide surface without wrinkles. Slides must be free of cracks, scratches, or contamination.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eMinimum Cellularity:\u003c\/strong\u003e Tissue specimens should contain \u0026gt;1,000 cells.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eTissue Quality:\u003c\/strong\u003e FFPE blocks must contain solid tumor tissue. Necrotic tumor tissue, fine needle aspiration (FNA) biopsies, and cytospin preparations may compromise staining quality.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eFixation:\u003c\/strong\u003e Tissues should be fixed in 10% neutral buffered formalin (NBF) for 18–24 hours.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eSectioning:\u003c\/strong\u003e Cut sections at 3–5 µm thickness using positively charged (adhesive) slides. Slide preparation within one week post-fixation is recommended.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong class=\"\"\u003eSection Mounting:\u003c\/strong\u003e Do not add any adhesives during the water bath flotation step. Place the specimen in the center of the slide's positive side. Remove excess water by vertically placing the slide on absorbent paper; gently tap to remove droplets. Do not wipe the slide with paper.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eDrying:\u003c\/strong\u003e Place slides on a 45°C hot plate for 30 minutes (air-drying for ≥1 hour is acceptable).\u003c\/div\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong\u003eII. Assay Method\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e1. Required Equipment\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eMicropipettes, constant-temperature drying oven, microwave oven, immunohistochemistry (IHC) hydrophobic barrier pen, antigen retrieval container, staining jars, timer, humidified incubation chamber, coverslips, fume hood, wash bottles, fluorescence microscope, 100 mL and 1,000 mL graduated cylinders, etc.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e2. Required Reagents\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eSterile deionized water (abs9259), xylene, ethanol (100%, 95%, 70%), 10% neutral buffered formalin, antigen retrieval solution, primary antibodies, polymer HRP-conjugated secondary antibodies (abs20216, abs50213), blocking solution, PBST, anti-fade mounting medium, etc.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e3. Reagent Preparation\u003c\/strong\u003e\u003c\/h4\u003e\n\u003col start=\"1\"\u003e\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eFluorophore Dilution:\u003c\/strong\u003e The fluorophore is supplied as a 200× stock solution. Dilute 1:200 in signal amplification reaction buffer to prepare the 1× working solution (prepare fresh immediately before use).\u003c\/div\u003e\u003c\/li\u003e\u003c\/ol\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e4. Detection Equipment\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eFluorescence microscope or whole-slide fluorescence scanning system. Excitation and emission filter sets for TSA monochromatic fluorophores should conform to the specifications listed in Table 7.\u003c\/div\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong class=\"\"\u003eIII. Protocol for FFPE Tissue Sections\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e1. Deparaffinization and Rehydration\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Immerse slides in fresh xylene for 10 min, repeat 3 times.\u003cbr\u003e(2) Gradient ethanol rehydration: 100% ethanol for 5 min; 95% ethanol for 5 min; 70% ethanol for 2 min.\u003cbr\u003e(3) Rinse in sterile water for 1 min, repeat 3 times.\u003cbr\u003e(4) Optional: Immerse in 10% NBF or 4% paraformaldehyde (PFA) for 10–30 min, followed by sterile water rinse for 1 min, repeat 3 times (perform based on sample quality).\u003cbr\u003e(5) Optional: Apply membrane permeabilization reagent for 15 min, wash in PBST for 3 min, repeat 3 times (generally not required).\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e2. Microwave Antigen Retrieval\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Place deparaffinized slides in an antigen retrieval container and submerge in 1× antigen retrieval working solution.\u003cbr\u003e(2) Heat in a microwave at high power until boiling.\u003cbr\u003e(3) Maintain at low power for 15 min (replenish buffer as needed to prevent tissue desiccation).\u003cbr\u003e(4) Remove and allow to cool naturally to room temperature.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e3. Quenching and Blocking\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove residual wash buffer from slides.\u003cbr\u003e(2) Encircle the specimen area with an IHC hydrophobic barrier pen. Apply peroxidase blocking solution to cover the specimen area, incubate for 10 min, then wash in PBST for 3 min, repeat 3 times.\u003cbr\u003e(3) Remove residual wash buffer. Apply blocking solution to cover the specimen area, incubate at room temperature for 60 min, then remove blocking solution.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e4. Primary Antibody Incubation\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove blocking solution from slides.\u003cbr\u003e(2) Apply diluted primary antibody solution to cover the specimen area using a micropipette.\u003cbr\u003e(3) Incubate in a humidified chamber with gentle agitation at room temperature for 1 h (optimize for individual antibodies) or overnight at 4°C.\u003cbr\u003e(4) Wash in 1× PBST for 3 min, repeat 3 times.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e5. Secondary Antibody Incubation\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove residual wash buffer from slides.\u003cbr\u003e(2) Apply polymer HRP-conjugated secondary antibody working solution to cover the specimen area.\u003cbr\u003e(3) Incubate in a humidified chamber at room temperature for 30 min.\u003cbr\u003e(4) Wash in 1× PBST for 3 min, repeat 3 times.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e6. Fluorescent Signal Amplification\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove residual wash buffer from slides.\u003cbr\u003e(2) Apply 50–100 µL of 1× fluorophore working solution (diluted 1:200 in signal amplification buffer) to cover the specimen area.\u003cbr\u003e(3) Incubate in a humidified chamber with gentle agitation at room temperature for 10–15 min.\u003cbr\u003e(4) Wash in 1× PBST at room temperature for 3 min, repeat 3 times.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e7. Subsequent Rounds of Staining (Proceed directly to Step 8 for single staining)\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) After each round, staining quality may be verified under a fluorescence microscope. Ensure slides are kept moist with PBST to prevent desiccation.\u003cbr\u003e(2) \u003cstrong\u003eElution:\u003c\/strong\u003e Remove residual wash buffer. Apply antibody elution buffer to cover the specimen area, incubate at 37°C for 20–30 min, then remove elution buffer (no additional quenching step required).\u003cbr\u003e(3) Repeat Steps 4–6.\u003cbr\u003e(4) For multiplex staining, repeat Step 7(1)–(3), then proceed to nuclear counterstaining and mounting.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e8. Nuclear Counterstaining and Mounting\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eApply 1× DAPI working solution to cover the specimen area, incubate at room temperature for 10 min. Wash in 1× PBST 3 times, 2 min each. Apply anti-fade mounting medium and cover with a coverslip, avoiding air bubbles. For long-term storage, seal the edges of the coverslip with clear nail polish.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e9. Image Acquisition and Analysis\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eObserve and analyze stained tissue sections under a fluorescence microscope.\u003c\/div\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong class=\"\"\u003eIV. Protocol for Frozen Tissue Sections (Requires Antibody Elution Buffer abs994)\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e1. Quenching, Permeabilization, and Blocking\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove frozen slides from the freezer, equilibrate to room temperature, wash in PBST for 3 min, repeat 3 times.\u003cbr\u003e(2) Optional: Immerse in 10% NBF or 4% PFA for 10–30 min, followed by sterile water rinse for 1 min, repeat 3 times (perform based on sample quality).\u003cbr\u003e(3) Optional: Apply membrane permeabilization reagent for 15 min, wash in PBST for 3 min, repeat 3 times (generally not required).\u003cbr\u003e(4) Encircle the specimen area with an IHC hydrophobic barrier pen. Apply peroxidase blocking solution to cover the specimen area, incubate for 10 min, then wash in PBST for 3 min, repeat 3 times.\u003cbr\u003e(5) Remove residual wash buffer. Apply blocking solution to cover the specimen area, incubate at room temperature for 60 min, then remove blocking solution.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e2. Primary Antibody Incubation\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove blocking solution from slides.\u003cbr\u003e(2) Apply diluted primary antibody solution to cover the specimen area using a micropipette.\u003cbr\u003e(3) Incubate in a humidified chamber with gentle agitation at room temperature for 1 h (optimize for individual antibodies) or overnight at 4°C.\u003cbr\u003e(4) Wash in 1× PBST for 3 min, repeat 3 times.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e3. Secondary Antibody Incubation\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove residual wash buffer from slides.\u003cbr\u003e(2) Apply HRP-conjugated secondary antibody working solution to cover the specimen area.\u003cbr\u003e(3) Incubate in a humidified chamber at room temperature for 30 min.\u003cbr\u003e(4) Wash in 1× PBST for 3 min, repeat 3 times.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e4. Fluorescent Signal Amplification\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) Remove residual wash buffer from slides.\u003cbr\u003e(2) Apply 50–100 µL of 1× fluorophore working solution (diluted 1:200 in signal amplification buffer) to cover the specimen area.\u003cbr\u003e(3) Incubate in a humidified chamber with gentle agitation at room temperature for 10 min.\u003cbr\u003e(4) Wash in 1× PBST at room temperature for 3 min, repeat 3 times.\u003cbr\u003e(5) Apply antibody elution buffer (abs994), incubate at 37°C for 15–20 min.\u003cbr\u003e(6) Rinse in sterile water once, then wash in 1× PBST for 2 min.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong class=\"\"\u003e5. Subsequent Rounds of Staining (Proceed directly to Step 6 for single staining)\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003e(1) After each round, staining quality may be verified under a fluorescence microscope. Ensure slides are kept moist with PBST to prevent desiccation.\u003cbr\u003e(2) Repeat Steps 2–4.\u003cbr\u003e(3) For multiplex staining, repeat Step 5, then proceed to nuclear counterstaining and mounting.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e6. Nuclear Counterstaining and Mounting\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eApply 1× DAPI working solution to cover the specimen area, incubate at room temperature for 10 min. Wash in 1× PBST 3 times, 2 min each. Apply anti-fade mounting medium and cover with a coverslip, avoiding air bubbles. For long-term storage, seal the edges of the coverslip with clear nail polish.\u003c\/div\u003e\n\u003ch4 class=\"\"\u003e\u003cstrong\u003e7. Image Acquisition and Analysis\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cdiv class=\"paragraph\"\u003eObserve and analyze stained tissue sections under a fluorescence microscope.\u003c\/div\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong class=\"\"\u003eV. Interpretation of Results\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col start=\"1\"\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003eAlterations in microwave antigen retrieval conditions, incubation time, or temperature may lead to erroneous results.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003eEach staining run must include both tissue positive controls and reagent negative controls.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003eIf the tissue positive control fails to demonstrate positive staining, the results for the entire batch of samples should be deemed invalid.\u003c\/div\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong\u003eVI. Product Performance Specifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col start=\"1\"\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong\u003eConformance:\u003c\/strong\u003e Using conformance tissue sections (including positive tissue controls and negative reagent controls), the corresponding immunohistochemical assay must yield positive staining in positive controls and negative staining in negative controls.\u003c\/div\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cdiv class=\"paragraph\"\u003e\n\u003cstrong class=\"\"\u003eIntra-batch Reproducibility:\u003c\/strong\u003e Using the same batch of reagents, three consecutive sections from the same tissue block must produce consistent staining results.\u003c\/div\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong\u003eVII. Fluorophore Information\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"table markdown-table\" data-v-46d91a25=\"\" data-v-b3358103=\"\"\u003e\u003cdiv class=\"table-container\" data-v-46d91a25=\"\"\u003e\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\" data-v-46d91a25=\"\"\u003e\n\u003cthead data-v-46d91a25=\"\"\u003e\u003ctr data-v-46d91a25=\"\"\u003e\n\u003cth align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eDye Name\u003c\/span\u003e\u003c\/th\u003e\n\u003cth align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eExcitation Wavelength (nm)\u003c\/span\u003e\u003c\/th\u003e\n\u003cth align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eEmission Wavelength (nm)\u003c\/span\u003e\u003c\/th\u003e\n\u003cth align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eSimilar Fluorophores\u003c\/span\u003e\u003c\/th\u003e\n\u003cth class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eRecommended Color Setting\u003c\/span\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody data-v-46d91a25=\"\"\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eDAPI\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e360\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e461\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eDAPI\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eBlue\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 480\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e450\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e480\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eOpal 480 \/ Aqua\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eCyan\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 520\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e490\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e520\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eFITC \/ AF488 \/ Opal 520\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eGreen\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 540\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e520\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e540\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eAF514 \/ Opal 540\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eYellow-Green\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 570\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e550\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e570\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eCy3 \/ AF555 \/ Opal 570\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eYellow\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 620\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e590\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e620\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eAF594 \/ Opal 620 \/ Texas Red\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eRed\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 650\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e630\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e650\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eCy5 \/ AF610 \/ Opal 650\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eFar-Red\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 690\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e640\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e670\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eAF647 \/ Cy5 \/ Opal 670\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNear-Infrared\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 700\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e680\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e702\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eAF680 \/ Cy5.5 \/ Opal 690\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eNear-Infrared\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eTSA Monochromatic Fluorophore 770\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e740\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003e780\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"left\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eCy7 \/ Opal 780\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd class=\"\" align=\"center\" data-v-46d91a25=\"\"\u003e\u003cspan style=\"font-size: 14px;\"\u003eInfrared\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003ch3 class=\"\"\u003e\u003cstrong\u003eVIII. Recommended Companion Products\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"table markdown-table\" data-v-46d91a25=\"\" data-v-b3358103=\"\"\u003e\u003cdiv class=\"table-container\" data-v-46d91a25=\"\"\u003e\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\" data-v-46d91a25=\"\"\u003e\n\u003cthead data-v-46d91a25=\"\"\u003e\u003ctr data-v-46d91a25=\"\"\u003e\n\u003cth style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eProduct Name\u003c\/th\u003e\n\u003cth class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody data-v-46d91a25=\"\"\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eFixation\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9179\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e4% Paraformaldehyde (Universal Tissue Fixative)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eEmbedding (Frozen Samples)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9756\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eOCT Embedding Medium\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e110 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eAntigen Retrieval (FFPE)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9342\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eTris-EDTA Antigen Retrieval Buffer (10×, pH 9.0)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eAntigen Retrieval (FFPE)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9248\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eCitrate Antigen Retrieval Buffer (50×)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eAntibody Elution (for samples unsuitable for heat-mediated elution, e.g., cells, frozen tissue, bone tissue)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs994\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eAntibody Elution Buffer (mIHC Grade)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eCell Permeabilization (required for intracellular targets)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9149\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eTriton X-100\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eEndogenous Peroxidase Quenching\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9333\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePeroxidase Blocking Solution (H₂O₂ Method)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eEndogenous Peroxidase Quenching\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9152\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eTween-20\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL \/ 2.5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eBlocking (serum matched to secondary antibody host)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs933\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eGoat Serum\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003eBlocking\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9157\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eBovine Serum Albumin (BSA)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePrimary Antibodies\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs1\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eIHC Primary Antibodies\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePrimary Antibodies\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9299\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePrimary\/Secondary Antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" rowspan=\"18\" align=\"left\" data-v-46d91a25=\"\"\u003eMulticolor Kits (contain TSA fluorophores, DAPI, mounting medium, secondary antibodies, signal amplification buffer)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50165\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (770 Enhanced) (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50166\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (770 Enhanced) (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50015\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50037\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (Plus) (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50031\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50038\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e7-Color mIHC Staining Kit (Plus) (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50014\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e6-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50049\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e6-Color mIHC Staining Kit (Plus) (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50030\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e6-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50048\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e6-Color mIHC Staining Kit (Plus) (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50013\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e5-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50029\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e5-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50012\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e4-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50028\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e4-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T \/ 50T \/ 100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50089\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e3-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50088\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e3-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50087\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e2-Color mIHC Staining Kit (Universal Anti-Mouse\/Rabbit Secondary)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50086\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003e2-Color mIHC Staining Kit (Anti-Rabbit Secondary)\u003c\/td\u003e\n\u003ctd style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" rowspan=\"2\" align=\"left\" data-v-46d91a25=\"\"\u003eLung Cancer Panel (includes primary antibodies)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50083\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eLung Cancer Tumor Microenvironment mIHC Detection Kit (I)\u003c\/td\u003e\n\u003ctd style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50084\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eLung Cancer Tumor Microenvironment mIHC Detection Kit (II)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" rowspan=\"3\" align=\"left\" data-v-46d91a25=\"\"\u003eBuffers\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9341\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePBST (10×, pH 7.4)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs9340\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePBST (1×, pH 7.4)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs962\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003ePBS Buffer (1×)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd class=\"\" style=\"width: 47.7603%;\" rowspan=\"2\" align=\"left\" data-v-46d91a25=\"\"\u003ePolymer HRP Secondary Antibodies\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs20216\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eHigh-Sensitivity Polymer HRP-Goat Anti-Rabbit IgG (H+L)\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-v-46d91a25=\"\"\u003e\n\u003ctd style=\"width: 7.32686%;\" align=\"left\" data-v-46d91a25=\"\"\u003eabs50213\u003c\/td\u003e\n\u003ctd class=\"\" style=\"width: 36.4534%;\" align=\"left\" data-v-46d91a25=\"\"\u003eHigh-Sensitivity Polymer HRP-Goat Anti-Rabbit\/Mouse IgG (H+L)\u003c\/td\u003e\n\u003ctd style=\"width: 8.41233%;\" align=\"center\" data-v-46d91a25=\"\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"paragraph\"\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv class=\"paragraph\"\u003eThe tissue microenvironment comprises a complex array of cellular constituents, whose phenotypes, functional states, abundances, and spatial distributions hold profound biological significance and clinical relevance. These parameters can be visualized in situ through antibody-based staining methodologies. While conventional immunohistochemistry (IHC) represents a standard approach for investigating tissue morphology and in situ protein expression, traditional single-marker IHC is inherently limited in its ability to elucidate the cellular composition, activation states, and intercellular relationships within complex tissue microenvironments—information that is critical for accurate disease diagnosis and therapeutic decision-making.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"paragraph\"\u003e\u003cstrong\u003eTyramide Signal Amplification (TSA) Technology\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"paragraph\"\u003eThe principle of TSA is analogous to conventional DAB chromogenic IHC. TSA utilizes horseradish peroxidase (HRP)-conjugated secondary antibodies to catalyze the conversion of added fluorophore-conjugated tyramide substrates into highly reactive intermediates. These activated tyramide radicals form stable covalent bonds with tyrosine residues present on target antigens, resulting in the permanent deposition of fluorescent signals directly at the epitope site. Following signal development, heat-mediated epitope retrieval (HIER) is employed to strip non-covalently bound primary\/secondary antibody complexes, thereby enabling sequential rounds of incubation with different primary antibodies and corresponding fluorophore-conjugated tyramide substrates. This iterative stripping-and-staining workflow facilitates robust multiplexed immunofluorescence labeling, allowing for the comprehensive spatial profiling of multiple biomarkers within a single tissue section.\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 540, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":42373449318475,"sku":"abs50100-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"anti-rabbit-and-mouse-hrp-dab-ihc-detection-kit-2-step-s0c2034","title":"Anti-Rabbit and Mouse HRP-DAB IHC detection kit (2-step)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs, Rb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eAnti-Rabbit and Mouse HRP-DAB IHC detection kit (2-step) utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml","offer_id":42902028451915,"sku":"S0C2034-5ml","price":145.0,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":42908258992203,"sku":"S0C2034-50ml","price":850.0,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":42902028517451,"sku":"S0C2034-100ml","price":1450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bffbdc2edb59489f96013d2cbd5e09bc.jpg?v=1788242519"},{"product_id":"hyper-tsa-ultra-multiplex-ihc-kit-abs50269","title":"HyperTSA Ultra Multiplex IHC Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e 1. Detection sample \u003cbr\u003e\u003c\/strong\u003e1 biopsy or surgically removed samples of different species; \u003cbr\u003e2 Use after tissue isolation 10% Neutral formalin fixed, fixed time 8-48 h Best; \u003cbr\u003e3 Recommended tissue section thickness 3-4 μm Use an anti-fall slide. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 2. Operation mode \u003c\/strong\u003e\u003cbr\u003e Hand dyeing \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Required experimental equipment, consumables and reagents \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1 Experimental equipment: \u003c\/strong\u003e\u003cbr\u003e Incubator, microwave oven, centrifuge, vortex mixer \u003cbr\u003e\u003cstrong\u003e2 Experimental consumables: \u003c\/strong\u003e\u003cbr\u003e Dyeing cylinder, incubation wet box, drying plate, anti-slide slide, cover slide, washing bottle, measuring cylinder, pipette, immunohistochemistry hydrophobic pen, timer, EP Tube, pipette tip, blue cap vial \u003cbr\u003e\u003cstrong\u003e3 Experimental reagents: \u003c\/strong\u003e\u003cbr\u003e Sterilized deionized water, xylene, ethanol ( 100% 、 95% 、 75% ）、  10% Neutral formalin, anti-fluorescence quenching sealant, Tween -20 ， Triton\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Working fluid preparation \u003c\/strong\u003e\u003cbr\u003e1 Fluorescent dye working solution \u003cbr\u003e         The dye in the kit is 100X Mother liquor, placed at room temperature before use 10min , reuse HyperTSA Signal amplification solution according to 1:100 Dilute and use (configure within half an hour before use, do not configure in advance). \u003cbr\u003e2 、 HyperTSA HRP Enzyme-labeled anti-mouse \/ Rabbit IgG Polymer secondary antibody working solution (3x)\u003cbr\u003e         With buffer ( TBS 、 PBS 、 TBST 、 PBST Either can) will 3X Enzyme-labeled anti-mouse \/ Rabbit IgG The polymer secondary antibody concentrate is diluted and prepared as a secondary antibody working solution (prepared within half an hour before use, do not prepare in advance). \u003cbr\u003e3 、 HyperTSA DAPI Working fluid \u003cbr\u003e         As per drop DAPI Concentrate plus 500 μL Ratio of buffer, dropwise HyperTSA DAPI Concentrate solution into buffer solution, mix well to obtain DAPI Working fluid. \u003cbr\u003e4 Antigen recovery solution working solution \u003cbr\u003e         Use deionized water to 50X The concentrate was prepared according to 1:50 The dilution was configured as an antigen retrieval working solution. \u003cbr\u003e5 、 HyperTSA  Fluorescent Eluent Working Solution ( 5X ） \u003cbr\u003e         Use deionized water to 5X The concentrate dilution was configured as a fluorescent eluent working solution. \u003cbr\u003e6 、 TBST\u003cbr\u003e         In TBS Add in 0.1% Twain of -20 And 0.03% of Triton Configured as working fluid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e V. Experimental operation \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e Note: Before starting the experiment, please confirm that other equipment, consumables, reagents, etc. required for the experiment have been prepared. Carefully read the [Working Solution Preparation] section and prepare the working solution as required. \u003cbr\u003e\u003c\/strong\u003e1 The paraffin sections are placed in 60℃ Baking in incubator 60 min ； \u003cbr\u003e2 , dewaxing and hydration: xylene (10 min)→ Xylene (10 min) →  Xylene (10 min)→ Anhydrous ethanol (5 min×2 Secondary )→95% Ethanol (5 min×1)→75% Ethanol (5 min) (You can also choose environmentally friendly tissue transparent liquid, refer to its instructions for specific operation) ;\u003cbr\u003e3 Distilled water flushing 5 min×2 Secondary; \u003cbr\u003e4 Antigen remediation: immerse the slice in the antigen remediation solution working liquid for microwave remediation and preheat 5 min , high fire 2 min , low fire 15 min ； \u003cbr\u003e5 Natural cooling at room temperature; \u003cbr\u003e6 Washing: Use TBST washing 3 Secondary , 5 min\/ Secondary; \u003cbr\u003e\u003cstrong\u003e First round of staining \u003c\/strong\u003e\u003cbr\u003e7 Blocking: Blocking solution is blocked at room temperature 15 min ； \u003cbr\u003e8 Primary antibody incubation: dropwise addition of primary antibody working solution 100 μl ， 37℃ incubation 1 h (or 4℃ overnight incubation); \u003cbr\u003e9 Washing: TBST washing 3 times, 5 min\/ Secondary; \u003cbr\u003e10 Secondary antibody incubation: dropwise addition of secondary antibody 100 μl ， 37℃ incubation 10 min ； \u003cbr\u003e11 Washing: TBST washing 3 Secondary , 5 min\/ Secondary; \u003cbr\u003e12 Fluorescence color development: dropwise addition of fluorescent dye working solution 100 μl , room temperature 5 min;\u003cbr\u003e13 Washing: TBST washing 3 times, 5 min\/ Secondary; \u003cbr\u003e14 Microwave treatment: repeating the steps 4-6 ； \u003cbr\u003e15 Sequential antibody staining: the first antibody staining is completed, and each subsequent antibody needs to repeat the steps 7-14 Complete all antibody staining sequentially; \u003cbr\u003e16 dropwise addition DAPI Working fluid 100 μl Staining, room temperature 5 min ； \u003cbr\u003e17 Washing: distilled water washing 3 times, 5 min\/ Secondary; \u003cbr\u003e18 Dropwise adding antifluorescence quenching sealing agent 100 μl , coverslip seal scan. \u003cbr\u003e19 After the first round of staining image scanning is completed, the slide is soaked in buffer solution to remove the coverslip (the soaking time varies according to the length of the scanning time of the seal). \u003cbr\u003e\u003cstrong\u003e20 Elution step: preheating the fluorescence eluent working liquid in a microwave oven over high heat 3 min Immersing the sections in the fluorescent eluent working fluid, microwave repair, low fire 15 min ； \u003c\/strong\u003e\u003cbr\u003e21 Natural cooling at room temperature; \u003cbr\u003e22 Washing: Use TBST washing 3 Secondary , 5 min\/ Secondary; \u003cbr\u003e\u003cstrong\u003e Second round of staining \u003c\/strong\u003e\u003cbr\u003e23 Repeat 7-18 Step 2 completes the second round of image acquisition. If necessary, multiple rounds of subsequent staining should be completed in sequence. \u003cbr\u003e24 Perform single-round image analysis as needed or register multiple rounds of images using software for super-multi-target analysis. \u003cbr\u003e* All the conditions in the experimental procedure are recommended, and the specific experimental conditions are adjusted according to the actual situation. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHypermultiplex immunohistochemical staining kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHyperTSA washable ultra-multiplex immunohistochemical staining kit is a scientific research tool for tissue space in situ detection. It innovatively combines tyramine signal amplification (TSA) technology with elutable technology. After each round of multiplex staining and imaging, the covalently bound fluorescent dye is eluted by elutable technology for the next round of multiplex staining and imaging. Image registration of multiple rounds of staining data can realize in situ labeling of supermultiplex protein targets in one slice, breaking through the limitation of traditional TSA technology (7-9 targets), and realizing the detection of 10-50 protein markers. Through the cyclic workflow of \"staining-imaging-elution-staining\", it can not only save tissue samples, but also reveal the spatial interaction relationship of cell subsets in complex tissues, which provides strong technical support for cutting-edge fields such as tumor microenvironment research and neural circuit analysis.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of dyeing:\u003c\/strong\u003e\u003cbr\u003eHyperTSA ultra-multiplex immunohistochemical staining kit innovatively integrates tyramine signal amplification (TSA) technology and reversible staining technology, providing researchers with an efficient and flexible multi-target detection solution. The core of the kit contains four kinds of HyperTSA fluorescent dyes with excellent spectral characteristics. With special signal amplification solution and high-efficiency polymer secondary antibody, it can realize accurate co-localization analysis of more than 10 protein markers. Its complete working system also includes: tissue antigen retrieval solution (suitable for multiple sample pretreatment needs), multifunctional blocking\/primary antibody diluent (effective in reducing background interference), high-purity DAPI concentrate (nuclear localization labeling), and revolutionary fluorescent dye eluent (achieving gentle dye removal).\u003cbr\u003eIn a single round of experiments, conventional TSA staining method can be used for multiplex staining. After the staining is completed, TSA fluorescent dye can be eluted with fluorescent dye eluent, so that the next round of staining can be carried out. After multiple rounds of staining, the detection of super-multiple targets on the same slice is realized.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 95.0001%; height: 238px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e25T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e50T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA 520\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e30μL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e60μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e120μL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e494\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e525\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA 570\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e30μL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e60μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e120μL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e570\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA 620\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e30μL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e60μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e120μL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e588\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e616\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA 690\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e30μL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e60μL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e120μL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e676\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e694\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 18px; text-align: center;\"\u003eHyperTSA DAPI\u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 18px; text-align: center;\"\u003e2mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 18px; text-align: center;\"\u003e2mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 18px; text-align: center;\"\u003e2mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e358\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e461\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA  Primary antibody diluent \/ blocking fluid \u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e35mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e70mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e140mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA HRP Enzyme-labeled anti-mouse \/ Rabbit IgG Polymer secondary antibody \u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e6mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e11mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e22mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA  Signal amplifier \u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e18mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e35mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e70mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA  Antigen retrieval solution ( 50X ） \u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e200mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 38.1328%; height: 22px; text-align: center;\"\u003eHyperTSA  Fluorescent eluate ( 5X ） \u003c\/td\u003e\n\u003ctd style=\"width: 10.0173%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003ctd style=\"width: 9.85562%; height: 22px; text-align: center;\"\u003e500mL\u003c\/td\u003e\n\u003ctd style=\"width: 10.0456%; height: 22px; text-align: center;\"\u003e1000mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.0997%; text-align: center;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. PH value has an influence on dyeing. The slide should be clean and free of acid and alkali pollution, otherwise it will affect the dyeing effect.\u003cbr\u003e2. Imaging the tissue in time after staining. If you need to store it, please avoid light.\u003cbr\u003e3. Pay attention to gentle operation in the process of taking out the coverslip after each round of dyeing to reduce stripping.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHypermultiplex immunohistochemical staining for paraffin tissue sections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eLong-term storage at-20℃, 2-8℃ after opening, sealed and protected from light. Valid for 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25T","offer_id":42905663471691,"sku":"abs50269-25T","price":1246.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":42905663504459,"sku":"abs50269-50T","price":2111.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42905663537227,"sku":"abs50269-100T","price":3413.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/14cbb790603147468b0b90ce1e96b7a9.png?v=1789873231"},{"product_id":"anti-rabbit-and-mouse-hrp-dab-ihc-secondary-antibody-2-step-s0c2034-a","title":"Anti-Rabbit and Mouse HRP-DAB IHC Secondary Antibody (2-step)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIHC-P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs, Rb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eAnti-Rabbit and Mouse HRP-DAB IHC Secondary Antibody (2-step) utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"5ml","offer_id":42908261023819,"sku":"S0C2034-A-5ml","price":120.0,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":42908261056587,"sku":"S0C2034-A-50ml","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":42908261089355,"sku":"S0C2034-A-100ml","price":1250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/138790f08c9b42b88a92971c8887b86c_0c088867-a4e4-4b45-86a7-bb2d5e5a0b4d.jpg?v=1788246165"},{"product_id":"s0d0006","title":"Hematoxylin Stain Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, room temperature as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is mainly used for staining the nuclei of tissue cell sections.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100ml","offer_id":43031149445195,"sku":"S0D0006-100ml","price":35.0,"currency_code":"USD","in_stock":true},{"title":"500ml","offer_id":43031149477963,"sku":"S0D0006-500ml","price":135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d106994c-b563-4b69-96a5-6f735ddfd368.png?v=1788501790"},{"product_id":"reagents-of-the-ihc-detection-kit-s0c1001-a","title":"Reagents of the IHC detection kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50ml","offer_id":43085935607883,"sku":"S0C1001-A-50ml","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4b22f22f-b413-4147-a926-37d27b5ba6e6.png?v=1788033783"},{"product_id":"new-seven-color-multi-label-immunofluorescence-kit","title":"Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003c!-- All URLs have been updated from www.absin.cn to www.absin.net --\u003e \u003cstrong\u003eI. Specimen requirements\u003c\/strong\u003e\u003cbr\u003e1. Formalin-fixed paraffin blocks or slides, large tissue sections or TMAs; paraffin seal must be intact.\u003cbr\u003e2. For slide specimens, tissue must adhere tightly to the slide without folds; slides must be free of cracks, scratches or stains.\u003cbr\u003e3. Tissue must contain \u0026gt;1 000 cells.\u003cbr\u003e4. Blocks must contain solid tumour tissue; necrotic tumour, fine-needle aspirates or cytospins will impair staining.\u003cbr\u003e5. Tissue must be fixed in 10 % neutral-buffered formalin for 18–24 h.\u003cbr\u003e6. Cut 4 µm sections on adhesive slides; prepare slides within one week after fixation.\u003cbr\u003e7. Do NOT use any adhesives in the water-bath; place tissue on the front centre of the slide. Drain vertically on absorbent paper and gently tap off droplets—never wipe the slide.\u003cbr\u003e8. Dry slides on a 45 °C hot plate for 30 min (air-dry ≥1 h if no hot plate is used).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eII. Assay procedure\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1. Equipment required\u003c\/strong\u003e: pipettes, oven, microwave, IHC pen, antigen-retrieval vessel, staining jars, timer, humid incubation box, coverslips, fume hood, wash bottle, fluorescence microscope, 100 mL \u0026amp; 1 000 mL cylinders, etc.\u003cbr\u003e\u003cstrong\u003e2. Reagents required\u003c\/strong\u003e: sterile de-ionised water (abs9259), xylene, ethanol (100 %, 95 %, 70 %), 10 % neutral-buffered formalin, primary antibodies, blocking buffer, TBST, etc.\u003cbr\u003e\u003cstrong\u003e3. Reagent preparation\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1)\u003c\/strong\u003e Fluorophore dilution: 100× stock dyes are diluted 1:100 in signal-amplification buffer (prepare fresh). DAPI 100× stock is diluted 1:100 in sterile water.\u003cbr\u003e\u003cstrong\u003e2)\u003c\/strong\u003e Secondary antibody: the kit supplies a mouse\/rabbit universal secondary; verify species compatibility (goat host).\u003cbr\u003e\u003cstrong\u003e4. Imaging\u003c\/strong\u003e: fluorescence microscope or whole-slide scanner equipped with filter sets matching the excitation\/emission spectra listed in the dye table.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIII. Paraffin-section protocol\u003c\/strong\u003e\u003cbr\u003e1. Deparaffinisation \u0026amp; rehydration\u003cbr\u003ea) Fresh xylene 10 min ×3.\u003cbr\u003eb) Ethanol gradient: 100 % 5 min → 95 % 5 min → 70 % 2 min.\u003cbr\u003ec) Sterile water rinse 1 min ×3.\u003cbr\u003ed) Optional post-fixation: 10 % neutral formalin or 4 % PFA 10–30 min, water rinse 1 min ×3.\u003cbr\u003ee) Optional permeabilisation: permeabilisation reagent 15 min, PBST 3 min ×3.\u003cbr\u003e\u003cbr\u003e2. Heat-induced epitope retrieval (HIER)\u003cbr\u003ea) Immerse slides in 1× antigen-retrieval buffer in microwave vessel.\u003cbr\u003eb) Microwave at high power until boiling.\u003cbr\u003ec) Maintain low power 15 min (top up liquid to prevent drying).\u003cbr\u003ed) Cool to room temperature (RT).\u003cbr\u003e\u003cbr\u003e3. Quenching \u0026amp; blocking\u003cbr\u003ea) Remove excess buffer.\u003cbr\u003eb) Circle tissue with IHC pen; cover with 3 % H₂O₂ 10 min, PBST 3 min ×3.\u003cbr\u003ec) Block with blocking buffer 10–30 min at RT with shaking; discard.\u003cbr\u003e\u003cbr\u003e4. Primary antibody incubation\u003cbr\u003ea) Remove blocking buffer.\u003cbr\u003eb) Add diluted primary antibody to cover tissue.\u003cbr\u003ec) Incubate 1 h at RT with shaking (optimise if necessary).\u003cbr\u003ed) 1×TBST 3 min ×2.\u003cbr\u003e\u003cbr\u003e5. Secondary antibody incubation\u003cbr\u003ea) Remove wash buffer.\u003cbr\u003eb) Add HRP-conjugated secondary to cover tissue.\u003cbr\u003ec) Incubate 10 min at RT in humid box.\u003cbr\u003ed) 1×TBST 3 min ×2.\u003cbr\u003e\u003cbr\u003e6. Fluorescent signal amplification\u003cbr\u003ea) Remove wash buffer.\u003cbr\u003eb) Add 100 µL 1× TSA fluorophore working solution (1:100 dilution) to cover tissue.\u003cbr\u003ec) Incubate 10 min at RT with shaking.\u003cbr\u003ed) 1×TBST 3 min ×3.\u003cbr\u003ee) Microwave retrieval; cool to RT.\u003cbr\u003ef) Sterile water rinse, then 1×TBST 2 min.\u003cbr\u003e\u003cbr\u003e7. Next staining cycle (skip for single colour)\u003cbr\u003ea) Check staining under microscope; keep tissue wet with TBST.\u003cbr\u003eb) Block 10–30 min (no quenching needed).\u003cbr\u003ec) Repeat steps 4–6.\u003cbr\u003ed) After final cycle proceed to nuclear staining \u0026amp; mounting.\u003cbr\u003e\u003cbr\u003e8. Nuclear staining \u0026amp; mounting\u003cbr\u003eAdd 1×DAPI working solution 5 min at RT. 1×TBST 2 min ×3. Apply antifade mounting medium and coverslip; seal edges with clear nail polish for long-term storage.\u003cbr\u003e\u003cbr\u003e9. Imaging \u0026amp; analysis: visualise on fluorescence microscope.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Fresh-frozen-section protocol (requires antibody-stripping buffer \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\u003cbr\u003e1. Quenching, permeabilisation \u0026amp; blocking\u003cbr\u003ea) Equilibrate frozen slides to RT; PBST 3 min ×3.\u003cbr\u003eb) Optional fixation: 10 % neutral formalin or 4 % PFA 10–30 min, water rinse 1 min ×3.\u003cbr\u003ec) Optional permeabilisation: permeabilisation reagent 15 min, PBST 3 min ×3.\u003cbr\u003ed) Circle tissue; 3 % H₂O₂ 10 min, PBST 3 min ×3.\u003cbr\u003ee) Block 10–30 min; discard.\u003cbr\u003e\u003cbr\u003e2. Primary antibody: same as paraffin step 4.\u003cbr\u003e3. Secondary antibody: same as paraffin step 5.\u003cbr\u003e4. TSA amplification\u003cbr\u003ea–d) identical to paraffin step 6a–d.\u003cbr\u003ee) Add antibody-stripping buffer (abs994) 15–20 min at 37 °C.\u003cbr\u003ef) Water rinse, then 1×TBST 2 min.\u003cbr\u003e\u003cbr\u003e5. Next cycle (skip for single colour)\u003cbr\u003ea–d) same logic as paraffin step 7.\u003cbr\u003e6. Nuclear staining \u0026amp; mounting: identical to paraffin step 8.\u003cbr\u003e7. Imaging \u0026amp; analysis.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eV. Interpretation\u003c\/strong\u003e\u003cbr\u003e1. Any change in retrieval, incubation time or temperature can produce erroneous results.\u003cbr\u003e2. Each run must include a tissue-positive control and a reagent-negative control.\u003cbr\u003e3. If the positive control fails, the entire batch is invalid.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eVI. Performance characteristics\u003c\/strong\u003e\u003cbr\u003e1. Accuracy: positive control tissue must stain positively; negative control must remain unstained.\u003cbr\u003e2. Within-batch reproducibility: triplicate sections stained with the same kit lot must yield identical patterns.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eVII. Spectral data\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eFluorophore\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003ePeak wavelength (nm)\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003eSpectrally equivalent commercial dyes\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003eSuggested pseudo-colour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEx\u003c\/td\u003e\n\u003ctd\u003eEm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDAPI\u003c\/td\u003e\n\u003ctd\u003e360\u003c\/td\u003e\n\u003ctd\u003e461\u003c\/td\u003e\n\u003ctd\u003eDAPI\u003c\/td\u003e\n\u003ctd bgcolor=\"#272dd1\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-480\u003c\/td\u003e\n\u003ctd\u003e450\u003c\/td\u003e\n\u003ctd\u003e480\u003c\/td\u003e\n\u003ctd\u003eOpal 480 \/ Aqua\u003c\/td\u003e\n\u003ctd bgcolor=\"#0c5c0d\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-520\u003c\/td\u003e\n\u003ctd\u003e490\u003c\/td\u003e\n\u003ctd\u003e520\u003c\/td\u003e\n\u003ctd\u003eFITC \/ AF 488 \/ Opal 520\u003c\/td\u003e\n\u003ctd bgcolor=\"#2dc26b\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-540\u003c\/td\u003e\n\u003ctd\u003e520\u003c\/td\u003e\n\u003ctd\u003e540\u003c\/td\u003e\n\u003ctd\u003eAF 514 \/ Opal 540\u003c\/td\u003e\n\u003ctd bgcolor=\"#f2f930\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-570\u003c\/td\u003e\n\u003ctd\u003e550\u003c\/td\u003e\n\u003ctd\u003e570\u003c\/td\u003e\n\u003ctd\u003eCy3 \/ AF 555 \/ Opal 570\u003c\/td\u003e\n\u003ctd bgcolor=\"#f1c40f\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-620\u003c\/td\u003e\n\u003ctd\u003e590\u003c\/td\u003e\n\u003ctd\u003e620\u003c\/td\u003e\n\u003ctd\u003eAF 594 \/ Opal 620 \/ Texas Red\u003c\/td\u003e\n\u003ctd bgcolor=\"#df534b\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-650\u003c\/td\u003e\n\u003ctd\u003e630\u003c\/td\u003e\n\u003ctd\u003e650\u003c\/td\u003e\n\u003ctd\u003eCy5 \/ AF 610 \/ Opal 650\u003c\/td\u003e\n\u003ctd bgcolor=\"#a30707\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-690\u003c\/td\u003e\n\u003ctd\u003e640\u003c\/td\u003e\n\u003ctd\u003e670\u003c\/td\u003e\n\u003ctd\u003eAF 647 \/ Cy5 \/ Opal 670\u003c\/td\u003e\n\u003ctd bgcolor=\"#7e0036\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-700\u003c\/td\u003e\n\u003ctd\u003e680\u003c\/td\u003e\n\u003ctd\u003e702\u003c\/td\u003e\n\u003ctd\u003eAF 680 \/ Cy5.5 \/ Opal 690\u003c\/td\u003e\n\u003ctd bgcolor=\"#843fa1\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTSA-770\u003c\/td\u003e\n\u003ctd\u003e740\u003c\/td\u003e\n\u003ctd\u003e780\u003c\/td\u003e\n\u003ctd\u003eCy7 \/ Opal 780\u003c\/td\u003e\n\u003ctd bgcolor=\"#ced4d9\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eVIII. Associated reagents\u003c\/strong\u003e (available at www.absin.net)\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\"\u003eCategory\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eProduct\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003eSize\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\"\u003eFixation\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e4 % paraformaldehyde (universal fixative)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\"\u003eEmbedding (frozen)\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eOCT compound\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e110 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"2\"\u003eAntigen retrieval (paraffin)\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eTris-EDTA buffer (10×, pH 9.0)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eSodium citrate buffer (50×)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\"\u003eAntibody stripping\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003emIHC antibody stripping buffer\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\"\u003ePermeabilisation\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eTriton X-100\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"2\"\u003ePeroxidase quenching\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eH₂O₂ blocking solution\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eTween-20\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL \/ 2.5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"2\"\u003eBlocking\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"2\"\u003ePrimary antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eValidated IHC antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eAntibody diluent\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"18\"\u003eMultiplex kits\u003cbr\u003e(TSA dyes, DAPI, mounting medium, secondary, amplification buffer)\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit (770-enhanced, anti-rabbit HRP)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit (770-enhanced, mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit plus (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e7-colour mIHC kit plus (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e6-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e6-colour mIHC kit plus (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e6-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e6-colour mIHC kit plus (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e5-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e5-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e4-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e4-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20\/50\/100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e3-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e3-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e2-colour mIHC kit (mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003e2-colour mIHC kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"2\"\u003eLung-cancer panels\u003cbr\u003e(primary antibodies included)\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eLung TME panel I\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eLung TME panel II\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e20 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.4129%;\" rowspan=\"3\"\u003eBuffers\u003c\/td\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs952\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003eTBST (10×)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL ×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003ePBST (1×, pH 7.4)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.9351%;\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"width: 41.2515%;\"\u003ePBS (1×)\u003c\/td\u003e\n\u003ctd style=\"width: 19.4439%;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe tissue microenvironment harbors a complex cellular composition whose phenotype, state, abundance, and spatial distribution carry profound biological significance and clinical value. These characteristics can be visualized in situ by antibody-based staining. Immunohistochemistry (IHC) is a routine technique for interrogating tissue architecture and in-situ protein expression; however, conventional single-color IHC reveals only one marker at a time, making it insufficient to depict the intricate cellular constituents, states, and inter-cellular relationships within the microenvironment—information that is indispensable for disease diagnosis and therapy. \u003cbr\u003e\u003cbr\u003eTyramide signal amplification (TSA) principle: analogous to the DAB chromogenic method used in standard IHC, TSA employs an HRP-conjugated secondary antibody. Horseradish peroxidase catalyzes the added fluorogenic tyramide substrate, generating highly reactive fluorescent tyramide radicals that covalently bind to tyrosine residues on nearby proteins, stably depositing fluorophore at the antigen site. Subsequent heat-mediated stripping removes non-covalently bound antibodies, allowing sequential rounds of primary antibody incubation with a different fluorophore-conjugated tyramide substrate. Iteration of this cycle enables high-plex multiplexed labeling.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 110px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"90%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 3.75641%; height: 44px;\" rowspan=\"2\" width=\"66\"\u003ePack size\u003c\/td\u003e\n\u003ctd style=\"width: 61.2435%; height: 22px; text-align: center;\" colspan=\"11\" width=\"624\"\u003eabs50037 (mouse\/rabbit) 7-color multiplex fluorescent IHC kit (plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 3.4391%; height: 22px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 4.95833%; height: 22px;\" width=\"66\"\u003eTSA-480\u003c\/td\u003e\n\u003ctd style=\"width: 5.08334%;\"\u003eTSA-520\u003c\/td\u003e\n\u003ctd style=\"width: 4.6827%; height: 22px;\" width=\"66\"\u003eTSA-570\u003c\/td\u003e\n\u003ctd style=\"width: 5.00313%; height: 22px;\" width=\"66\"\u003eTSA-620\u003c\/td\u003e\n\u003ctd style=\"width: 5.26606%; height: 22px;\"\u003eTSA-690\u003c\/td\u003e\n\u003ctd style=\"width: 5.26597%; height: 22px;\"\u003eTSA-770\u003c\/td\u003e\n\u003ctd style=\"width: 7.11855%; height: 22px;\" width=\"75\"\u003eDAPI 100×\u003c\/td\u003e\n\u003ctd style=\"width: 6.59617%; height: 22px;\" width=\"77\"\u003eSignal amplification buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.15705%; height: 22px;\" width=\"86\"\u003ePoly-HRP-conjugated secondary antibody (goat, mouse\/rabbit universal)\u003c\/td\u003e\n\u003ctd style=\"width: 6.67309%; height: 22px;\" width=\"117\"\u003eAntifade mounting medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 3.75641%; height: 22px;\"\u003e100 reactions\u003c\/td\u003e\n\u003ctd style=\"width: 3.4391%; height: 66px;\" rowspan=\"3\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 4.95833%; height: 22px;\" width=\"66\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.08334%;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 4.6827%; height: 22px;\" width=\"66\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.00313%; height: 22px;\" width=\"66\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26606%; height: 22px;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26597%; height: 22px;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 7.11855%; height: 22px;\" width=\"75\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 6.59617%; height: 22px;\" width=\"77\"\u003e60 mL\u003c\/td\u003e\n\u003ctd style=\"width: 7.15705%; height: 22px;\" width=\"86\"\u003e60 mL\u003c\/td\u003e\n\u003ctd style=\"width: 6.67309%; height: 22px;\" width=\"117\"\u003e5 mL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 3.75641%; height: 22px;\"\u003e50 reactions\u003c\/td\u003e\n\u003ctd style=\"width: 4.95833%; height: 22px;\" width=\"66\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.08334%;\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 4.6827%; height: 22px;\" width=\"66\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.00313%; height: 22px;\" width=\"66\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26606%; height: 22px;\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26597%; height: 22px;\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 7.11855%; height: 22px;\" width=\"75\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 6.59617%; height: 22px;\" width=\"77\"\u003e30 mL\u003c\/td\u003e\n\u003ctd style=\"width: 7.15705%; height: 22px;\" width=\"86\"\u003e30 mL\u003c\/td\u003e\n\u003ctd style=\"width: 6.67309%; height: 22px;\" width=\"117\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 3.75641%; height: 22px;\"\u003e20 reactions\u003c\/td\u003e\n\u003ctd style=\"width: 4.95833%; height: 22px;\" width=\"66\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.08334%;\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 4.6827%; height: 22px;\" width=\"66\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.00313%; height: 22px;\" width=\"66\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26606%; height: 22px;\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 5.26597%; height: 22px;\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"width: 7.11855%; height: 22px;\" width=\"75\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 6.59617%; height: 22px;\" width=\"77\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"width: 7.15705%; height: 22px;\" width=\"86\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"width: 6.67309%; height: 22px;\" width=\"117\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003eKit components: TSA monomeric fluorophore 480, TSA monomeric fluorophore 520, TSA monomeric fluorophore 570, TSA monomeric fluorophore 620, TSA monomeric fluorophore 690, TSA monomeric fluorophore 770, signal amplification buffer, mouse\/rabbit universal HRP-conjugated secondary antibody, antifade mounting medium, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is intended \u003cem\u003eexclusively for immunohistochemistry\u003c\/em\u003e; any other application is prohibited.\u003cbr\u003e2. Operation must be performed \u003cem\u003eonly by qualified laboratory personnel\u003c\/em\u003e.\u003cbr\u003e3. Appropriate personal protective equipment must be worn to \u003cem\u003eprevent contact with skin and eyes\u003c\/em\u003e.\u003cbr\u003e4. Reagents may lose activity after the stated expiry date; \u003cem\u003edo not use beyond expiration\u003c\/em\u003e.\u003cbr\u003e5. Mixing any staining component of this kit with products from other manufacturers may lead to \u003cem\u003eunpredictable staining artefacts\u003c\/em\u003e.\u003cbr\u003e6. Incomplete de-paraffinisation can \u003cem\u003ecompromise staining quality\u003c\/em\u003e.\u003cbr\u003e\u003col\u003e\n\u003cli\u003eTo avoid false-negative or false-positive results, \u003cem\u003ealways run a positive tissue control and a negative reagent control in parallel\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003eAll waste generated during use must be disposed of in accordance with the \u003cem\u003eRegulations on the Administration of Medical Waste\u003c\/em\u003e.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43212772245579,"sku":"abs50037-20T","price":710.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43212772278347,"sku":"abs50037-100T","price":2592.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43212772311115,"sku":"abs50037-50T","price":1664.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/daa6bc86b2864242995acdf6b079fd52.jpg?v=1789740038"},{"product_id":"new-seven-color-multi-label-immunofluorescence-kit-abs50038","title":"Absin 7-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. 2. Use of secondary antibody: The kit is an anti-rabbit secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50037) for universal secondary antibody to pika and rabbit.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 480, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 690, TSA monochromatic fluorescent dye 770, signal amplification reaction solution, anti-rabbit HRP labeled secondary antibody, anti-fluorescence quenching sealing tablet, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43212772343883,"sku":"abs50038-20T","price":710.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43212772376651,"sku":"abs50038-100T","price":2592.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43212772409419,"sku":"abs50038-50T","price":1664.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bec39440ab6643f5919d35b1a5adb149_b3347863-8fbc-43c8-9c73-c54d3381f4b7.jpg?v=1789740040"},{"product_id":"six-color-multi-labeled-immunofluorescence-staining-kit-abs50048","title":"Absin 6-Color mlHC Kit(plus) (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3\n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20\n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto’s thyroiditis. Nature Communications. 2022 Feb 9\n4. Dabbs David J. 诊断免疫组织化学(M). 北京：北京大学医学出版社，2008.9.\n5. 美】Ed Harlow, David Lane. 抗体技术指南(M). 北京：科学出版社，2002:79-80,105.\n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58.\n7. 钱帮国. 焦磊. 多标记免疫荧光染色及多普成像技术在组织学研究中的应用. 中国组织化学与细胞化学杂志, 2017 (4): 373-382.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1. For TSA dyes, please use signal amplification reaction solution diluted at 1:100 to prepare the dye working solution (currently used and prepared); DAPI prepared the working solution by diluting it with sterile water at a ratio of 1:100\u003cbr\u003e2 Use of secondary antibody: The reagent kit is anti rabbit secondary antibody, please verify whether the species of the primary antibody match before the experiment. Pika universal secondary antibody, please refer to (# abs50049)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cell composition in tissue microenvironment. The phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. With the help of antibody staining, it can be presented in situ. Immunohistochemical staining is a common technology to study tissue morphology and in situ protein expression. Conventional IHC detection can only show a single index, which is difficult to show the cell composition, state and relationship in the complex tissue microenvironment, and this information is essential for the diagnosis and treatment of disease\u003cbr\u003etyramine signal amplification technology principle: similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP labeled secondary antibody. HRP catalyzes the addition of the fluorescein substrate of the system to produce activated fluorescent substrate. The activated substrate can covalently bind with tyrosine on the antigen to make the stable covalently bound fluorescein on the sample. After that, the non covalently bound antibody is washed away by the thermal repair method, and the second round of incubation is carried out after changing a primary antibody, and then changing another fluorescein substrate. In this way, multiple labeling can be realized\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This reagent kit is only used for immunohistochemistry and is not intended for any other purpose\u003cbr\u003e2 This reagent kit is only for professional use\u003cbr\u003e3 Appropriate protective measures should be taken to avoid contact between reagents and skin and eyes\u003cbr\u003e4 Reagents that exceed their expiration date may have reduced activity, therefore they should not be used\u003cbr\u003e5 If the staining components of this reagent kit are mixed with products from other companies, abnormal situations may occur during the staining process\u003cbr\u003e6 Incomplete dewaxing can easily affect the dyeing effect\u003cbr\u003e7 In order to prevent possible false negative and false positive results, both positive and negative controls should be conducted simultaneously during the experiment\u003cbr\u003e8 All waste generated from the use of this reagent kit should be disposed of in accordance with the Medical Waste Management Regulations.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes need to be protected from light, Store at 2-8 ℃, 1 year from receipt\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUsed for immunohistochemical staining of tissues, paraffin sections, and TMA chips.Can also be used for frozen sections and cell slides with abs994 Antibody eluent (mIHC only)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43212772606027,"sku":"abs50048-20T","price":653.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43212772638795,"sku":"abs50048-100T","price":2385.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43212772671563,"sku":"abs50048-50T","price":1533.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/61d401b455c44481a35a940d454e168e.jpg?v=1789740051"},{"product_id":"absin-6-color-ihc-kit","title":"Absin 6-Color IHC Kit (plus) (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. For TSA dye, please use the signal amplification reaction solution to dilute it at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: This kit is matched with a universal secondary antibody for pika and rabbits, which is not suitable for mouse tissues. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50048) for mouse tissue samples.\u003c\/p\u003e\n\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 690, TSA monochromatic fluorescent dye 770, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43212772737099,"sku":"abs50049-20T","price":653.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43212772769867,"sku":"abs50049-100T","price":2385.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43212772802635,"sku":"abs50049-50T","price":1533.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c85e732c927e4d89ab59ab248568e08e.jpg?v=1789740053"},{"product_id":"four-color-multi-label-immunofluorescence-kit-abs50168","title":"Absin 4-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: The kit is matched with anti-rabbit secondary antibody. Please verify whether the species of primary antibody matches before the experiment. Please refer to (# abs50012) for universal secondary antibody to pika and rabbit.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, anti-rabbit HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43213662715979,"sku":"abs50168-20T","price":533.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43213662748747,"sku":"abs50168-100T","price":2025.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43213662781515,"sku":"abs50168-50T","price":1231.0,"currency_code":"USD","in_stock":true}]},{"product_id":"four-color-multi-label-immunofluorescence-kit-abs50167","title":"Absin 4-Color IHC Kit B (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al. Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: This kit is matched with a universal secondary antibody for pika and rabbits, which is not suitable for mouse tissues. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50168) for mouse tissue samples.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43213663043659,"sku":"abs50167-20T","price":533.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43213663076427,"sku":"abs50167-100T","price":2025.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43213663109195,"sku":"abs50167-50T","price":1231.0,"currency_code":"USD","in_stock":true}]},{"product_id":"new-seven-color-multi-label-immunofluorescence-kit-abs50166","title":"Absin 7-Color IHC Kit (Anti-Rabbit\u0026mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] EdHarlow, DavidLane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n3. Stack, E.C. etaI., Multiplexedimmunohistochemistry, imaging, andquantitation: areview, \nwithanassessmentofTyramidesignaIamplification, multispectraIimagingandmultiplexanalysis.Methods, 201470 \n（1）:46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Histochemistry and Cytochemistry \nJournal, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eⅠ. Sample requirements\u003c\/strong\u003e\u003cbr\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded with solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30 minutes (the natural air-drying time of the glass slide is not less than 1h).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅡ. Inspection method\u003c\/strong\u003e\u003cbr\u003e1. Required instruments and equipment: pipette, constant temperature drying oven, microwave oven, immunohistochemical pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, bottle washing, fluorescence microscope, measuring cylinder 100ml, measuring cylinder 1000ml, etc.\u003cbr\u003e2. Required reagents: sterilized deionized water, xylene, ethanol (100%, 95%, 70%), 10% neutral formalin, antigen repair primary antibody, blocking solution, TBST (item number: abs952), antibody diluent (item number: abs9299), etc.\u003cbr\u003e3. Reagent preparation:\u003cbr\u003e(1) Dilution of fluorescent dyes: all dyes (including TSA monochromatic fluorescent dye 770 component A) are 100 × mother liquor, and the signal amplification reaction solution is diluted at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e(2) Dilution of TSA770 dye component B: Component B is 100 × mother liquor, and dilute 1 × PBS or antibody diluent at 1: 100 to prepare TSA770 dye component B working solution (now prepared).\u003cbr\u003e4. Detection equipment: fluorescence microscope or fluorescent whole-film scanning equipment. The excitation and emission filters applicable to TSA monochromatic fluorescent dyes should comply with the recommendations in the table.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅢ. Paraffin sectioning operation steps\u003c\/strong\u003e\u003cbr\u003e1. Dewaxing and Hydration\u003cbr\u003e(a) Fresh xylene dip tablets for 10 min, repeated 3 times.\u003cbr\u003e(b) Gradient ethanol impregnation tablets: 100% for 5 min; 95% 5 min; 70% 2 min.\u003cbr\u003e(c) Wash the tablet with sterilized water for 1 min and repeat it three times.\u003cbr\u003e(d) 10% neutral formalin or 4% paraformaldehyde dip tablets for 10-30min, sterilized water wash tablets for 1min, and repeat 3 times. (Optional, depending on sample quality)\u003cbr\u003e(e) Dropwise add film breaking agent to permeate for 15 min, soak with TBST for 3 min, and repeat for 3 times. (Optional, generally not necessary)\u003cbr\u003e2. Microwave repair antigen\u003cbr\u003e(a) Place the dewaxed and hydrated glass slide in a repair cup and immerse it with antigen repair solution 1 × working solution.\u003cbr\u003e(b) Place the repair cup in a microwave oven and bring it to a high boil.\u003cbr\u003e(c) Maintain low fire for 15 minutes (pay attention to fluid rehydration to prevent excessive evaporation from causing dry tablets).\u003cbr\u003e(d) Remove room temperature and naturally cool to room temperature.\u003cbr\u003e3. Quenching and Blocking\u003cbr\u003e(a) Remove that residual wash liquid on the slide.\u003cbr\u003e(b) Circle the sample area on the glass slide with a histochemical pen, add 3% hydrogen peroxide dropwise to cover the sample area, incubate for 10 minutes, immerse in TBST for 3 minutes, and repeat 3 times.\u003cbr\u003e(c) Remove that residual lotion liquid on the glass slide, add blocking liquid dropwise, cover the sample area, moisturize at room temperature and shake for 10-30min, and remove the blocking liquid.\u003cbr\u003e4. Primary antibody incubation\u003cbr\u003e(a) Remove the blocking fluid from the slide.\u003cbr\u003e(b) Submerge the sample area by adding the diluted primary antibody solution dropwise with a pipette.\u003cbr\u003e(c) Moisturizing and shaking incubation at room temperature for 1h (optimization and adjustment need to be made for different antibodies).\u003cbr\u003e(d) The slides were dipped with 1 × TBSTbuffer for 3 min and repeated once.\u003cbr\u003e5. Secondary antibody incubation\u003cbr\u003e(a) Remove the residual wash solution on the slide.\u003cbr\u003e(b) Directly add HRP secondary antibody working solution dropwise to immerse the sample area.\u003cbr\u003e(c) Incubate at room temperature with moisture for 10 min.\u003cbr\u003e(d) The slides were dipped with 1 × TBSTbuffer for 3 min and repeated once.\u003cbr\u003e6. Fluorescence staining amplifies signal\u003cbr\u003e(a) Remove the residual wash solution on the slide.\u003cbr\u003e(b) Immerse the sample area by adding 1 × 100 ul of dye working solution dropwise on the glass slide with a pipette (diluted 1: 100 with signal amplification solution).\u003cbr\u003e(c) Incubate at room temperature with moisture shaking for 10 min.\u003cbr\u003e(d) 1 × TBSTbuffer dip slides and dip them at room temperature for 3 min. Repeat 3 times.\u003cbr\u003e(e) Microwave repair, natural cooling at room temperature to room temperature.\u003cbr\u003e(f) Wash the tablets once with sterilized water and dip the tablets in 1 × TBSTbuffer for 2 minutes.\u003cbr\u003e7. A new round of dyeing (single dyeing can be carried out directly to step 9)\u003cbr\u003e(a) After each round of staining, a fluorescence microscope can be used to confirm the staining. Pay attention to covering the sample with TBST to prevent drying.\u003cbr\u003e(b) Blocking: Remove the residual lotion solution on the glass slide, add the blocking solution dropwise, cover the sample area, moisturize at room temperature and shake for 10-30min, and remove the blocking solution. (No quenching step required)\u003cbr\u003e(c) Repeat steps 4-6\u003cbr\u003e(d) Repeat step 7 for multiple rounds of dyeing, and after completion of a)-c), dyeing nuclei and sealing are carried out. (Last round staining 770 fluorescent dye component A)\u003cbr\u003e8. TSA770 Dye Component B\u003cbr\u003eAdd 50-100ul 1 × TSA770 component B working solution dropwise, immerse the sample area, and incubate at room temperature for 10-30min (the staining time should be judged according to the staining results). The slides were dipped 3 times with 1 × TBST for 3 minutes each time.\u003cbr\u003e9. Dyeing and sealing\u003cbr\u003eAdd 1 × DAPI working solution dropwise to the sample, immerse the sample area, and incubate at room temperature for 5 minutes. The slides were dipped 3 times with 1 × TBST for 2 minutes each time. Then add anti-fluorescence quenching sealing tablets dropwise, and seal the tablets with coverslips to avoid bubbles. Please seal the edges of coverslips with transparent nail polish for long-term storage.\u003cbr\u003e10. Read the film, observe and analyze the stained tissue slice under a fluorescence microscope.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅣ. Frozen section operation steps (antibody eluate abs994 is required)\u003c\/strong\u003e\u003cbr\u003e1. Quenching, permeation, sealing\u003cbr\u003e(a) Remove the frozen slides from the refrigerator, return to room temperature, soak in PBST for 3 min, and repeat 3 times\u003cbr\u003e(b) 10% neutral formalin or 4% paraformaldehyde dipping tablets for 10-30min, washing tablets with sterilized water for 1min, repeating 3 times. (Optional, depending on sample quality)\u003cbr\u003e(c) Dropwise add membrane breaking agent for permeation for 15 min, soak PBST for 3 min, and repeat 3 times. (Optional, generally not necessary)\u003cbr\u003e(d) Circle the sample area on the glass slide with a histochemical pen, add 3% hydrogen peroxide dropwise to cover the sample area, incubate for 10 minutes, soak with PBST for 3 minutes, and repeat 3 times.\u003cbr\u003e(e) Remove that residual lotion liquid on the glass slide, add blocking liquid dropwise, cover the sample area, moisturize at room temperature and shake for 10-30min, and remove the blocking liquid.\u003cbr\u003e2. Primary antibody incubation\u003cbr\u003e(a) Remove the closure on the slide.\u003cbr\u003e(b) Submerge the sample area by adding the diluted primary antibody solution dropwise with a pipette.\u003cbr\u003e(c) Moisturizing and shaking incubation at room temperature for 1h (optimization and adjustment need to be made for different antibodies).\u003cbr\u003e(d) The slides were dipped with 1 × TBSTbuffer for 3 min and repeated once.\u003cbr\u003e3. Secondary antibody incubation\u003cbr\u003e(a) Remove the residual wash solution on the slide.\u003cbr\u003e(b) Directly add HRP secondary antibody working solution dropwise to immerse the sample area.\u003cbr\u003e(c) Incubate at room temperature with moisture for 10 min.\u003cbr\u003e(d) The slides were dipped with 1 × TBSTbuffer for 3 min and repeated once.\u003cbr\u003e4. Fluorescence staining amplification signal\u003cbr\u003e(a) Remove the residual wash solution on the slide.\u003cbr\u003e(b) Immerse the sample area by adding 1 × 100 ul of component A dye working solution dropwise on the glass slide with a pipette (diluted 1: 100 with signal amplification solution).\u003cbr\u003e(c) Incubate at room temperature with moisture shaking for 10 min.\u003cbr\u003e(d) 1 × TBSTbuffer dip slides and dip them at room temperature for 3 min. Repeat 3 times.\u003cbr\u003e5. A new round of dyeing (single dyeing can be carried out directly to step 9)\u003cbr\u003e(a) After each round of staining, a fluorescence microscope can be used to confirm the staining. Pay attention to covering the sample with TBST to prevent drying.\u003cbr\u003e(b) Blocking: Remove the residual lotion solution on the glass slide, add the blocking solution dropwise, cover the sample area, moisturize at room temperature and shake for 10-30min, and remove the blocking solution. (No quenching step) c) Repeat steps 4-6\u003cbr\u003e(d) Repeat step 7 for multiple rounds of dyeing, and after completion of a)-c), dyeing nuclei and sealing are carried out. (Last round staining 770 fluorescent dye component A)\u003cbr\u003e6. TSA770 Dye Component B\u003cbr\u003eAdd 50-100ul 1 × TSA770 component B working solution dropwise, immerse the sample area, and incubate at room temperature for 10-30min (the staining time should be judged according to the staining results). The slides were dipped 3 times with 1 × TBST for 3 minutes each time.\u003cbr\u003e7. Dyeing and sealing\u003cbr\u003eAdd 1 × DAPI working solution dropwise to the sample, immerse the sample area, and incubate at room temperature for 5 minutes. The slides were dipped 3 times with 1 × TBST for 2 minutes each time.\u003cbr\u003eThen add anti-fluorescence quenching sealing tablets dropwise, and seal the tablets with coverslips to avoid bubbles. Please seal the edges of coverslips with transparent nail polish for long-term storage.\u003cbr\u003e8. Read the film, observe and analyze the stained tissue slice under a fluorescence microscope.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅤ. Description of results\u003c\/strong\u003e\u003cbr\u003e1. The changes of microwave repair antigen, incubation time and temperature may all lead to wrong results.\u003cbr\u003e2. In each staining process, tissue positive control and reagent negative control experiments must be carried out at the same time.\u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test results of this batch of samples should be judged to be invalid.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅥ. Product performance indicators\u003c\/strong\u003e\u003cbr\u003e1. Conformity: Take the conforming tissue slice (including positive tissue slice and negative reagent control), and after the corresponding immunohistochemical test, the positive control staining result is positive, and the negative control staining result is negative.\u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect 3 sections of the same tissue. The staining results should be consistent.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cdiv\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250509\/81df88a510874f06b1257dd75d1f5978.png\" alt=\"\" width=\"701\" height=\"200\"\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eKit Components\u003cbr\u003eTSA monochromatic fluorescent dye 480, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 690, TSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution, anti-pika universal HRP labeled secondary antibody, anti-fluorescence quenching sealing tablet, DAPI\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and not for other purposes.\u003cbr\u003e2. This kit is limited to professional use.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact between the reagent and the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43213663371339,"sku":"abs50166-20T","price":737.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43213663404107,"sku":"abs50166-100T","price":2649.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43213663436875,"sku":"abs50166-50T","price":1698.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bec39440ab6643f5919d35b1a5adb149_2e9da04b-badd-4d84-aade-8cb9d15396c8.jpg?v=1789743885"},{"product_id":"new-seven-color-multi-label-immunofluorescence-kit-abs50165","title":"Absin 7-Color IHC Kit (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 \nXin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \nQian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 \n4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \nEd Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: The kit is an anti-rabbit secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50166) for universal secondary antibody to pika and rabbit.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 480, TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 690, TSA monochromatic fluorescent dye 770 component A, TSA monochromatic fluorescent dye 770 component B, signal amplification reaction solution, anti-rabbit HRP labeled secondary antibody, anti-fluorescence quenching sealing tablet, DAPI.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":43213663764555,"sku":"abs50165-20T","price":737.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43213663797323,"sku":"abs50165-100T","price":2649.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43213663830091,"sku":"abs50165-50T","price":1698.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bec39440ab6643f5919d35b1a5adb149_a98d1799-52ff-4820-a479-d000ff39ddc8.jpg?v=1789743884"},{"product_id":"three-color-multiplex-fluorescent-immunohistochemical-staining-kit-b-pika-universal-secondary-antibody","title":"Absin 3-Color IHC Kit B (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9.\u003cbr\u003e2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n\u003cbr\u003e3. Stack, E.C., etc., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with anassessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n\u003cbr\u003e4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dilution of fluorescent dye: the dye is 100 × mother liquor, and the signal amplification reaction solution is diluted at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: The kit is a universal secondary antibody for pika and rabbits. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003ca href=\"https:\/\/www.absin.cn\/stripping-buffer\/abs994.html\"\u003eabs994\u003c\/a\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 650, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":43213664125003,"sku":"abs50104-100T","price":720.0,"currency_code":"USD","in_stock":true}]},{"product_id":"three-color-multiplex-fluorescent-immunohistochemical-staining-kit-b-anti-rabbit-secondary-antibody","title":"Absin 3-Color IHC Kit B (Anti-Rabbit Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. DabbsDavidJ. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9.\u003cbr\u003e2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \n\u003cbr\u003e3. Stack, E.C., etc., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with anassessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n\u003cbr\u003e4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dilution of fluorescent dye: the dye is 100 × mother liquor, and the signal amplification reaction solution is diluted at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100.\u003cbr\u003e2. Use of secondary antibody: The kit is an anti-rabbit secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment.\u003cbr\u003e\u003cstrong\u003e3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:\u003ca href=\"https:\/\/www.absin.cn\/stripping-buffer\/abs994.html\"\u003eabs994\u003c\/a\u003e)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 650, signal amplification reaction solution, anti-rabbit HRP labeled secondary antibody, anti-fluorescence quenching sealing tablet, DAPI\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003cbr\u003e2. This kit is restricted to professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003cbr\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":43213664354379,"sku":"abs50103-100T","price":720.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.antbioinc.com\/collections\/ihc-detection-kit.oembed?page=5","provider":"AntBio","version":"1.0","type":"link"}