{"title":"Flow Cytometry","description":null,"products":[{"product_id":"human-fcr-blocking-reagent-mouse-anti-human-cd1632-human-antibody-against-cd64","title":"Human FcR Blocking Reagent (Mouse anti-human CD16\/32 \u0026 human antibody against CD64)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAnti-human CD16\/CD32\/CD64\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFc receptor blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS pH7.4, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFc receptor blocking\u003c\/td\u003e\n\u003ctd\u003e3μg (1μl\/test) per million cells in 100μl volume\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eFcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":41677708165195,"sku":"S0F0002-50T","price":120.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41677708197963,"sku":"S0F0002-100T","price":200.0,"currency_code":"USD","in_stock":true},{"title":"200T","offer_id":41677708230731,"sku":"S0F0002-200T","price":285.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":43083536629835,"sku":"S0F0002-500T","price":583.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e7b9019745ce4834a8b62ad4b5727f77_4036dd9d-c551-45e4-977e-353bd32d2d74.jpg?v=1789502571"},{"product_id":"human-fcr-blocking-reagent","title":"Human FcR Blocking Reagent","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFc receptor blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein G\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e5 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS pH7.4, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFCM\u003c\/td\u003e\n\u003ctd\u003e20μl per 10^7 cells in 1ml volume\u003c\/td\u003e\n\u003ctd\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eFcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining. Human FcR Blocking Reagent is compatible with flow cytometric staining with anti-human CD16, CD32, and CD64 antibodies. \u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":41677739688011,"sku":"S0F0008-50T","price":120.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41677739720779,"sku":"S0F0008-100T","price":200.0,"currency_code":"USD","in_stock":true},{"title":"200T","offer_id":41677739753547,"sku":"S0F0008-200T","price":285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8a97979265c94738a612e86ff120f553_f559431b-5b66-4c7d-961e-d1d0627ded9d.jpg?v=1787954675"},{"product_id":"human-fcr-blocking-reagent-human-antibodies-against-cd163264","title":"Human FcR Blocking Reagent (human antibodies against CD16\/32\/64)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAnti-human CD16\/CD32\/CD64，Fc Blocker, FcγR blocker, Fc inhibitor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFc receptor blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePredicted Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS pH7.4, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFc receptor blocking\u003c\/td\u003e\n\u003ctd\u003e3μg (1μl\/test) per million cells in 100μl volume\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eFcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003eIf the customer uses the detection antibody with human species after the blocking step, please make sure to use the labeled primary antibody. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":41677820559435,"sku":"S0F0012-50T","price":120.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41677820592203,"sku":"S0F0012-100T","price":200.0,"currency_code":"USD","in_stock":true},{"title":"200T","offer_id":41677820624971,"sku":"S0F0012-200T","price":285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/fca98d9fdf0745c7b279f3e893cdca6d_4d13ae0f-9ce4-421a-a4d4-be2e92cef4d7.jpg?v=1789578072"},{"product_id":"starter-magsep-separator-l-s0d3001","title":"Starter MagSep Separator (L)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807340619,"sku":"S0D3001-1pc","price":870.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4e98b27a03a64378afbc1724cdc8bfff.png?v=1788080444"},{"product_id":"starter-magsep-separator-4l-s0d3002","title":"Starter MagSep Separator (4L)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807373387,"sku":"S0D3002-1pc","price":1875.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2c519df4aea041ef82c29d7e92c66909.png?v=1788080446"},{"product_id":"starter-magsep-separator-s-s0d3003","title":"Starter MagSep Separator (S)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15–35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSeparators and columns are sensitive to heat and aggressive solvents, such as acetone. Do not autoclave separators or the MultiStand. Separators and the MultiStand can be cleaned with a soft cleansing tissue and a mild detergent, and disinfected using 70% ethanol. Do not drop Separators. Do not use if damage is visible.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807406155,"sku":"S0D3003-1pc","price":360.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4b0750454b1b417f8a24dadfc209425c.png?v=1788080447"},{"product_id":"starter-magsep-separator-8s-s0d3004","title":"Starter MagSep Separator (8S)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15–35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807438923,"sku":"S0D3004-1pc","price":1425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6b48891127ef403ca1660786f66c3588.png?v=1788080449"},{"product_id":"multistand-s0d3005","title":"MultiStand","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore MultiStands dry at 15–35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":41887807471691,"sku":"S0D3005-1pc","price":225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/00dcf52dfa2e445ca97b44de7c0f3958.png?v=1788080452"},{"product_id":"starter-magsep-separator-l-kit-s0d3006","title":"Starter MagSep Separator (L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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MagSep Separator (4L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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MagSep Separator (S) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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MagSep Separator (8S) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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MagSep Separator (S\u0026L) kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore separators dry at 15-35 °C. Do not store separators and MultiStand under a corrosive atmosphere, for example, in a chemical hood. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Separation system is developed to separate human and animal cells. Moreover it can be used for the separation of plant protoplasts, bacteria, cell organelles, and other bioparticles. The material to be separated, for example, cells, is first magnetically labeled with superparamagnetic NanoBeads. After magnetic labeling, cells are passed through a column which is placed in the strong permanent magnet of the separator. The ferromagnetic spheres in the column amplify the magnetic field by 10,000-fold, thus inducing a high gradient. Unlabeled cells pass through while magnetically labeled cell are retained within the column. After removal of the column from the magnetic field, the retained fraction can be eluted. Both fractions, labeled and non-labeled, are completely recovered.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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Separation Columns","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1x108 magnetically labeled cells in 2x109 total cells.Recommended sample size for leukocytes: 105 - 108 labeled cells in 107 - 2x109 total cells.Note: Column capacity may decrease when separating cells larger than lymphocytes.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore columns dry at +10 to +35 °C.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe column Technology is based on the use of NanoBeads, Columns, and Separators. Separation have been developed for the gentle isolation of NanoBead-labeled cells. As NanoBeads are extremely small, superparamagnetic particles, a high-gradient magnetic field is required to retain the labeled cells. Separation contains an optimized matrix to generate this strong magnetic field when placed in a permanent magnet, such as the Starter MagSep Separator (L) or Starter MagSep Separator (4L).\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e25 L Separation Columns and plungers, sterile packed\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25 Columns","offer_id":43088254959691,"sku":"S0D3011-25 Columns","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/16c6c6d3220643789910924c9a51ad63.jpg?v=1788084190"},{"product_id":"cd4-nanobeads-mouse-ruo-s0k0003","title":"CD4 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads conjugated with an anti-mouse CD4 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD4⁺ cells are magnetically labeled using CD4 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD4⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD4⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD4⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD4⁺ cells must be subjected to a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD4 antigen is expressed on most thymocytes and mature helper T cells, certain NKT cell subsets, and a small fraction of dendritic cell subsets. In CD4\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ehelper T cells, CD4 acts as a co-molecule to assist the TCR heterodimer in recognizing MHC class II\/peptide complexes. CD4 antigen is present on approximately 90% of thymocytes, 25% of splenic cells, and 55% of lymph node cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517841995,"sku":"S0K0003-1ml","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":42301372727371,"sku":"S0K0003-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ebe3156a992c4fb586128e55fa1492fb.jpg?v=1788094853"},{"product_id":"starter-magsep-separation-buffer-s0d3018","title":"Starter MagSep Separation  Buffer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:12.0pt;color:#404040\"\u003eStarter MagSep Separation Buffer (pH 7.2) contains\u003cbr\u003ephosphate buffered saline (PBS), bovine serum\u003cbr\u003ealbumin (BSA), EDTA, and azide.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:12.0pt;color:#404040\"\u003eStore protected fromlight at +2 to +8 ℃. Once opened, store for max. 21 days. Do not freeze.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"500ml","offer_id":42301380001867,"sku":"S0D3018-500ml","price":50.0,"currency_code":"USD","in_stock":true},{"title":"250ml","offer_id":42301379969099,"sku":"S0D3018-250ml","price":30.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8dc566e6-88f3-447c-b2c2-68e117fddfa8.png?v=1789470031"},{"product_id":"cd14-nanobeads-humanruo-s0k0004","title":"CD14 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD14 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD14⁺ cells are magnetically labeled using CD14 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD14⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD14⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD14⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD14 Nanobeads are used for the positive selection or depletion of human monocytes and macrophages from umbilical cord blood or peripheral blood mononuclear cells (PBMCs), as well as from pleural effusions, ascitic fluid, synovial fluid, or various tissues such as the spleen and lymph nodes. The CD14 antigen is part of the LPS receptor complex. Because CD14 lacks a cytoplasmic domain, antibody binding to CD14 does not trigger signal transduction. CD14 is strongly expressed on most monocytes and macrophages and weakly expressed on neutrophils and some myeloid dendritic cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":43030517743691,"sku":"S0K0004-1mL","price":283.33,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42301380984907,"sku":"S0K0004-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/53dd2b10efc44282bb4ec12673057a19.jpg?v=1788112965"},{"product_id":"starter-magsep-separation-rack-s0d3019","title":"Starter MagSep Separation Rack","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore racks at +10 to +35 °C.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1pc","offer_id":42301381705803,"sku":"S0D3019-1pc","price":5.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/cc32b987-9459-4d94-81ba-6f08bee23b1a.png?v=1788112974"},{"product_id":"cd8-nanobeads-mouseruo-s0k0005","title":"CD8 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with anti-mouse CD8 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD8⁺ cells are magnetically labeled using CD8 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive fraction containing CD8⁺ cells must be further separated through a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#404040\"\u003eCD8 Nanobeads\u003c\/span\u003e\u003cspan\u003eThese beads are designed for positive selection or depletion of murine cytotoxic T cells from lymphoid organs, blood, or tissues. The magnetic beads react with the immunoglobulin-like domain of the CD8α chain. The CD8α chain is expressed as a heterodimer with CD8β on cytotoxic T cells that express either the T cell receptor (TCR) α\/β or TCR γ\/δ, as well as on most thymocytes. As a homodimer composed of two CD8α chains, the CD8 antigen is present on most NK cells, the majority of intraepithelial lymphocytes in the intestine, some activated CD4+CD8+ T cells, and on CD8+ T cells in thymus-deficient mice.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517612619,"sku":"S0K0005-1ml","price":533.33,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42301424074827,"sku":"S0K0005-2mL","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/79082a3bd2a0436d8e65a20921136259.jpg?v=1788130962"},{"product_id":"cd19-nanobeads-humanruo-s0k0006","title":"CD19 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD19 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD19⁺ cells are magnetically labeled using CD19 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD19⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after depletion of CD19⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD19⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD19⁺ cells should be subjected to a second separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD19 Nanobeads are designed to isolate human B cells based on the expression of the CD19 antigen. CD19 is a 95–120 kDa glycosylated transmembrane protein that plays a crucial role in the signal transduction pathways regulating B lymphocyte development, activation, and differentiation. CD19 is expressed on cells of the B-cell lineage, from the early committed pro-B cell stage through the pre-B cell stage, including most malignant B cells. Its expression is downregulated during differentiation into plasma cells. In addition, CD19 is also expressed on follicular dendritic cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":42301461790795,"sku":"S0K0006-1ml","price":285.0,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":42301461823563,"sku":"S0K0006-2ml","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/bab27e7feca64df394d24dfb4d989dfa.jpg?v=1788141868"},{"product_id":"human-th1th2-cytokine-kitflow-cytometry-multiplex-bead-assay-s0q1001","title":"Human Th1\/Th2 Cytokine Kit（Flow Cytometry Multiplex Bead Assay）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-2、IL-4、IL-5、IL-6、IL-10、TNF-α、IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8℃ as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: \u0026lt;5%；\u003cbr\u003eInter-assay: \u0026lt;10%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;2.0pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.5pg\/ml - 6000pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70% - 130%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Cytometric Bead Array (CBA) technology enables the capture of soluble analytes or analyte panels using beads with known sizes and fluorescence intensities, thereby allowing the detection of analytes via flow cytometry.Each capture bead in the CBA kit is conjugated with a specific antibody. The detection reagent provided with the kit is Streptavidin-Phycoerythrin, and the intensity of the fluorescent signal generated by this reagent is proportional to the amount of bound analyte.When the capture beads and detection reagent are incubated together with the test sample containing target analytes, sandwich complexes are formed. Detection of these complexes by flow cytometry allows for the identification of particles exhibiting the fluorescence characteristics of both the beads and the detection reagent.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":42301475618891,"sku":"S0Q1001-1x96T","price":2000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/50e738d54ef544de8f9f51b9ddde961b_a066b399-1103-4d7b-8006-9edfb75b449a.png?v=1789632011"},{"product_id":"non-human-primates-nhp-fcr-blocking-reagent-rhesus-cynomolgus-baboon-s0f0050","title":"Non human primates (NHP) FCR Blocking Reagent (Rhesus, Cynomolgus, Baboon)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFc receptor blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMk\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS pH7.4, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFc receptor blocking\u003c\/td\u003e\n\u003ctd\u003e3μg (1μl\/test) per million cells in 100μl volume\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eFcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":42302083465291,"sku":"S0F0050-50T","price":175.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42302083498059,"sku":"S0F0050-100T","price":300.0,"currency_code":"USD","in_stock":true},{"title":"200T","offer_id":42302083530827,"sku":"S0F0050-200T","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/356b2b0144764edc9b7b4a874784b172_883463a1-c456-4972-be1b-2ec242534228.jpg?v=1790564413"},{"product_id":"dead-cell-removal-set-ruo-s0k0009","title":"Dead Cell Removal Set, (RUO)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1.\u003c\/span\u003e\u003cspan\u003eDextran-coated iron oxide magnetic nanoparticles coupled with Annexin V His-tagged protein, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2.\u003c\/span\u003e\u003cspan\u003eBuffer specifically for the dead cell removal kit.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e0.5 mL magnetic beads: suitable for a total cell count of 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1×10⁹ and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThe Dead Cell Removal Set can identify a specific structure on the plasma membranes of apoptotic and dead cells. During dead cell removal, cells are first magnetically labeled with Dead Cell Removal Nanobeads, and then the cell suspension is passed through a separation column. The magnetically labeled dead cells are retained within the column, while the unlabeled live cells flow through it; thus, dead cells are removed from this cell fraction. After removing the separation column from the magnetic field, the magnetically retained dead cells can be eluted as the positively selected cell fraction. Using Dead Cell Removal Nanobeads, even early apoptotic cells with intact plasma membranes can be effectively removed.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eReducing flow cytometry sorting time by removing dead cellsRemoving dead cells from cell culturesImproving Immunocytochemical Analysis of Frozen CellsPreparation of a viable single-cell suspension from tissuesRemoving dead cells from a sperm sampleRemove dead cells before and after cell transfection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe Dead Cell Removal Set is a rapid and simple method for removing dead cells from cell cultures or tissue preparations. This reagent contains ready-to-use nanomagnetic beads and a binding buffer, which are used to magnetically label cellular debris, dead cells, and dying cells. By magnetic separation, the magnetically labeled material is removed, allowing pure live cells to be obtained in a short time.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eUsing S\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSeparation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage \/ Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCount the total number of cells\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400×g, 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend every 1×10⁷ cells in 90 μL of 1× Binding Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe 20× Binding Buffer must be diluted only with sterile double-distilled water to make 1× Binding Buffer, then add 1% BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Dead Cell Removal NanoBeads per 1×10⁷ cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The binding of Dead Cell Removal NanoBeads requires Ca²⁺. The presence of the ion chelator EDTA will affect binding.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL per 1×10⁷ cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix thoroughly and incubate at 20–25℃ for 15 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e(Optional) If necessary, add 1× binding buffer to the cell suspension to bring the volume to at least 500 μL for separation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the S-type separation column in the magnetic field of the corresponding magnetic separator, and rinse the column with 500 μL of buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLoad the cell suspension onto the separation column and collect the flow-through (live cells).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect live cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the column with buffer three times, 500 μL each time. Add the next wash only after the liquid in the column has completely drained, and combine all the effluents into the negative fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect live cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRemove the separation column from the magnetic field and place it over a collection tube.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 1 mL of buffer to the column, then quickly and firmly push down with the accompanying plunger to elute the magnetically labeled positive cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor higher purity, use a new column and repeat the separation once.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the labeled cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eWith L\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSeparation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage \/ Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCount the total number of cells\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after AO\/PI staining\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400×g, 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 90 μL of 1× Binding Buffer per 1×10⁷ cells to resuspend them\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe 20× Binding Buffer must be diluted with sterile double-distilled water to 1× Binding Buffer before adding 1% BSA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Dead Cell Removal NanoBeads per 1×10⁷ cells\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe binding of Dead Cell Removal NanoBeads requires Ca²⁺. The presence of the ion chelator EDTA will affect the binding\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL per 1×10⁷ cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix thoroughly and incubate at 20–25℃ for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e(Optional) If necessary, add 1× binding buffer to the cell suspension to bring the volume to at least 500 μL for separation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the L-shaped sorting column in the magnetic field of the corresponding magnetic separator, and rinse the column with 3 mL of buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the sorting column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLoad the cell suspension onto the sorting column and collect the flow-through (live cells).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect live cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the column with buffer three times, 3 mL each time. Add the next wash only after the liquid in the column has completely drained. Combine all the effluent as the negative fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect live cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRemove the sorting column from the magnetic field and place it over the collection tube.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 5 mL of buffer to the column, then quickly and firmly push down with the accompanying plunger to elute the magnetically labeled positive cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor higher purity, use a new column and repeat the sorting once.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the labeled cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302111744075,"sku":"S0K0009-1mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8b911a950f0d4dcc9c70ecb7f7e6986b_e702c542-1f37-4e68-96b9-c3e9d59f81e9.png?v=1788778834"},{"product_id":"anti-f480-nanobeads-mouseruo-s0k0012","title":"Anti-F4\/80 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnets conjugated with an anti-mouse F4\/80 monoclonal antibody, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, allowing up to 200 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, allowing up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eUsing Anti-F4\/80 Nanobeads, mouse anti‑F4\/80\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells are subjected to magnetic labeling. Subsequently, the cell suspension is loaded into a separation column positioned within the sorter's magnetic field. The magnetically labeled F4\/80…\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells remain retained within the column, while unlabeled cells elute; this fraction constitutes the F4\/80-depleted fraction.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eThe negative fraction following cell sorting. After removing the sorting column from the magnetic field, the F4\/80‑positive cells that were magnetically captured…\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCells can be eluted to obtain the positive‑selection fraction. If higher purity is required, it contains F4\/80.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eThe positively sorted cell fraction must be subjected to a second round of sorting on a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eF4\/80 is a member of the epidermal growth factor (EGF) transmembrane 7 (TM7) family, consisting of a seven-transmembrane molecule and a large extracellular domain containing multiple EGF-like modules. F4\/80 is considered one of the most specific cell surface markers of murine macrophages. Constitutive high expression of F4\/80 is observed on the surface of most tissue-resident macrophages, including splenic macrophages, microglia in the brain, Kupffer cells in the liver, and Langerhans cells in the skin. Furthermore, F4\/80 expression is also regulated according to the physiological state of the cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302112301131,"sku":"S0K0012-1mL","price":535.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302112333899,"sku":"S0K0012-2mL","price":920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7fd3e8b476d347b6a496a6ad4641ca34.jpg?v=1788174152"},{"product_id":"cd3-nanobeads-humanruo-s0k0011","title":"CD3 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD3 monoclonal antibody.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 sorting runs\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD3 Nanobeads are used to magnetically label human CD3⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD3⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD3⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD3⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD3⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD3 is expressed on the surface of all T cells and is associated with the T-cell receptor. In human peripheral blood lymphocytes, 70–80% are CD3+, and in thymocytes, 65–85% are CD3+. The antigenic epitope recognized by CD3 Nanobeads is located on the CD3ε chain.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302114299979,"sku":"S0K0011-1mL","price":285.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302114332747,"sku":"S0K0011-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e0d8a9b1868547838ef9f1a0046ebceb.jpg?v=1788177630"},{"product_id":"cd8-nanobeads-humanruo-s0k0010","title":"CD8 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD8 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD8⁺ cells are magnetically labeled using CD8 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD8⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD8 antigen forms a complex with the T-cell receptor and serves as a co-molecule on CD8+ cytotoxic T cells, assisting the TCR heterodimer in recognizing MHC class I–peptide complexes. The CD8 molecule consists of an α\/β heterodimer or an α\/α homodimer. It is highly expressed on cytotoxic T cells and weakly expressed on some NK cells. CD8 is present on most thymocytes and approximately one-third of peripheral blood T cells. CD8+ cytotoxic T cells play a crucial role in eliminating virus-infected cells and tumor cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":43030516990027,"sku":"S0K0010-1mL","price":300.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302114725963,"sku":"S0K0010-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/be2c1ea3e1e14ee287d90f97830f0c6a.jpg?v=1788177634"},{"product_id":"cd4-nanobeads-humanruo-s0k0013","title":"CD4 Nanobeads, human（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD4 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003etotal cells, and can perform up to 50 separations\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD4⁺ cells are magnetically labeled using CD4 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD4⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD4⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD4⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD4⁺ cells must be further separated using a second column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD4 NanoBeads are designed to isolate human cells based on the expression of the CD4 antigen. CD4 is a 55 kDa protein that is highly expressed on helper T cells in the thymus and peripheral blood. In helper T cells, CD4 functions as a co-molecule, assisting the TCR heterodimer in recognizing MHC class II–peptide complexes. CD4 is also expressed on monocytes and dendritic cells, though at lower levels than in CD4+ helper T cells. When bound to certain chemokine receptors, CD4 serves as the primary high-affinity receptor for HIV.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":42302115217483,"sku":"S0K0013-1ml","price":300.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302115250251,"sku":"S0K0013-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6603e7615e5a4b1197d3ed41f7eeecf8.jpg?v=1788177637"},{"product_id":"fixable-viability-dye-452-s0b88801","title":"Fixable Viability Dye 452","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 452 is excited by the Violet laser (with an excitation maximum of 405 nm) and has fluorescence emission maximum at 452 nm, Fixable Viability Dye 452 has similar emission to Brilliant Violet 421™. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 452 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, Each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 452 Stock Solution for each 0.5~1 ml of cell suspension\/PBS (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 452, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Human PBMCs (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302121279563,"sku":"S0B88801-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b2a8612df79240b796c4e85779ec6ee3.jpg?v=1788177839"},{"product_id":"fixable-viability-dye-545-s0b88802","title":"Fixable Viability Dye 545","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 545 is excited by the Violet laser (with an excitation maximum of 405 nm) and has fluorescence emission maximum at 545 nm, Fixable Viability Dye 545 has similar emission to Brilliant Violet 480™, Amcyan, V500.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 545 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 545 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 545, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302121836619,"sku":"S0B88802-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3830abfb226b4b1da53b16be46264aa2.jpg?v=1788177844"},{"product_id":"fixable-viability-dye-583-s0b88803","title":"Fixable Viability Dye 583","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 583 is excited by the Yellow-Green laser (with an excitation maximum of 568 nm) and has fluorescence emission maximum at 583 nm, Fixable Viability Dye 583 has similar emission to PE, PI. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 583 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 583 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 583, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302122295371,"sku":"S0B88803-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3f1834ec54ad4a15970b2317712415ba.jpg?v=1788177846"},{"product_id":"fixable-viability-dye-515-s0b88804","title":"Fixable Viability Dye 515","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 515 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 515, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302122786891,"sku":"S0B88804-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8e4fc8f41efe481eb162a06d151a6f40.jpg?v=1788177850"},{"product_id":"fixable-viability-dye-777-s0b88805","title":"Fixable Viability Dye 777","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 777 is excited by the Red laser (with an excitation maximum of 750 nm) and has fluorescence emission maximum at 777 nm, Fixable Viability Dye 777 has similar emission to APC-Cy7. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 777 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 777 Stock Solution for each 1~2 ml of cell suspension\/PBS (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 777, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Human PBMCs (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302123212875,"sku":"S0B88805-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/90283b9ab4a44624be00043cc1151e0d.jpg?v=1788177856"},{"product_id":"fixable-viability-dye-662-s0b88806","title":"Fixable Viability Dye 662","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 662 is excited by the Red laser (with an excitation maximum of 640 nm) and has fluorescence emission maximum at 662 nm, Fixable Viability Dye 662 has similar emission to APC. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 662 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 662 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 662, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302123606091,"sku":"S0B88806-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b2da507a8981407d991009121277023c.jpg?v=1788177858"},{"product_id":"fixable-viability-dye-448-s0b88807","title":"Fixable Viability Dye 448","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 448 is excited by the UV laser (with an excitation maximum of 350 nm) and has fluorescence emission maximum at 448 nm, Fixable Viability Dye 448 has similar emission to DAPI. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 448 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 448 Stock Solution for each 0.5~1 ml of cell suspension (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 448, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Human PBMC (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302124195915,"sku":"S0B88807-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/be7063d57eac4215b14e89081e27badc.jpg?v=1788177862"},{"product_id":"fixable-viability-dye-740-s0b88808","title":"Fixable Viability Dye 740","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 740 is excited by the Red laser (with an excitation maximum of 615 nm) and has fluorescence emission maximum at 740 nm, Fixable Viability Dye 740 has similar emission to Alexa Fluor® 700.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 740 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 740 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 740, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302124621899,"sku":"S0B88808-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/67a3399fe8eb471c92737d42ff0f32d5.jpg?v=1788177865"},{"product_id":"fixable-viability-dye-575-s0b88809","title":"Fixable Viability Dye 575","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 575 is excited by the Violet laser (with an excitation maximum of 405 nm) and has fluorescence emission maximum at 600 nm, Fixable Viability Dye 575 has similar emission to Brilliant Violet 605™. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 575 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 575 Stock Solution for each 1~2 ml of cell suspension\/PBS (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 575, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to BALB\/c mouse bone marrow Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302125211723,"sku":"S0B88809-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d5061239ccd84484aacfe314b2c78356.jpg?v=1788177868"},{"product_id":"fixable-viability-dye-620-s0b88810","title":"Fixable Viability Dye 620","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 620 is excited by the Yellow laser (with an excitation maximum of 561 nm) and has fluorescence emission maximum at 600 nm, Fixable Viability Dye 620 has similar emission to PE-CF594, PI. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 620 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 620 Stock Solution for each 1~2 ml of cell suspension\/PBS (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 620, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302125604939,"sku":"S0B88810-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/14020c7bcd704b58b4323b903ec2f904.jpg?v=1788177871"},{"product_id":"fixable-viability-dye-700-s0b88811","title":"Fixable Viability Dye 700","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 700 is excited by the Red laser (with an excitation maximum of 631 nm) and has fluorescence emission maximum at 700 nm, Fixable Viability Dye 700 has similar emission to Alexa Fluor® 700.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 700 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.Wash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 1 µl of Fixable Viability Dye 700 Stock Solution for each 0.5~1 ml of cell suspension\/PBS (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Resuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 700, Incubate the mixture for 10-15 minutes at room temperature protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Resuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302126096459,"sku":"S0B88811-150μg","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f18f81c2932048819ad89d21ccfd35b8.jpg?v=1788177875"},{"product_id":"mouse-nave-cd4-t-cell-isolation-kit-column-free-s0k0014","title":"Mouse Naïve CD4 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Naïve CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-target cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. The non-target cells are marked by the antibody‑bead complex, while the target\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the sorter's magnetic field. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecells remain in the supernatant. This supernatant constitutes the\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecell-enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecell fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their native, unmanipulated state are sorted from a single-cell suspension of fresh mouse spleen and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eMouse naive CD4⁺ T cells (initial CD4-positive T lymphocytes) are mature CD4⁺ T cells that have not yet encountered specific antigens and have not undergone activation, proliferation, or differentiation. By flow cytometry, their canonical phenotype is defined as CD4⁺CD44⁻CD62L⁺. CD62L (L-selectin) is a key receptor for lymph node homing, highly expressed on quiescent naive T cells; upon cell activation or exposure to mechanical or temperature stress, it is rapidly cleaved and shed by the ADAM17 metalloprotease. CD44 is an adhesion molecule characteristic of activated and memory T cells, and is barely expressed on resting naive CD4⁺ T cells. Based on these features, naive T cells (CD44⁻CD62L⁺), central memory T cells (CD44⁺CD62L⁺), and effector memory T cells (CD44⁺CD62L⁻) can be precisely distinguished. Cell purity is a critical prerequisite for functional assays involving naive CD4⁺ T cells. This kit employs immunomagnetic negative selection: biotinylated antibodies specifically label non-target cells, including B cells, CD8⁺ T cells, myeloid cells, and activated\/memory T cells, which are then selectively removed using streptavidin-coated magnetic beads. Throughout the process, the target naive CD4⁺ T cells remain unlabeled and untouched, thereby maximizing preservation of their quiescent phenotype, viability, and differentiation potential. This approach efficiently yields high-purity, highly viable, unactivated \"untouched\" naive CD4⁺ T cells, suitable for various experimental applications such as in vitro polarization, cell activation assays, and studies of immune mechanisms.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px;font-weight: bold\"\u003eNote\u003c\/span\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003eThis kit does not include sorting buffer; please provide it separately. The corresponding Stater product number is:\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\"\u003eS0D3018\u003c\/a\u003e.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThe spleen was harvested from mice, passed through a 40-μm sterile filter to prepare a single-cell suspension, and subjected to red blood cell lysis using Red Blood Cell Lysis Buffer (without fixative, 1X) (Cat: S0D9002) prior to cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThroughout the grinding, filtration, and washing steps, use pre-cooled reagents and handle samples gently; avoid vigorous vortexing. It is recommended to count cells after staining with AO\/PI. For splenic cells, centrifuge at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eUsing pre-cooled 100 μL\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eResuspend to 1 × 10⁷\u003c\/span\u003e\u003cspan\u003ecell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust the reagents proportionally based on the sample volume; retain a pre-sorting sample to test the Naïve CD4 ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10⁷\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse Naïve CD4 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gently pipette up and down 2–3 times to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at 2–8°C for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake the Streptavidin beads for 5–30 seconds to mix thoroughly.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Streptavidin beads to the sample.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at 2–8°C for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, the mixture can be gently pipetted 2-3 times to mix thoroughly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn this step, the magnetic beads may settle at the bottom. You can gently tap the tube to mix them after 2.5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2-8℃ for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd pre-cooled\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eMagSep Separation Buffer\u003c\/span\u003e\u003cspan\u003eto the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling heavily at the bottom.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdjust the total volume to 2.5 mL (for a 5 mL flow cytometry tube).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdjust the total volume to 7.5 mL (for a 15 mL centrifuge tube).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand to allow the magnetic beads to adhere to the tube wall.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSingle-well magnetic rack adsorption (3 min).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMulti-well magnetic rack adsorption (5 min).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic rack and pour the sample into a new collection tube to collect the sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The sorted cells can be centrifuged at 400g for 7 minutes for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan\u003e0.1-1 × 10⁸\u003c\/span\u003e\u003cspan\u003eCells (0.1–1 mL) are recommended to be used with a 5 mL single-hole magnetic stirrer bar.\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) The total adsorption volume is 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2-5 ×10⁸\u003c\/span\u003e\u003cspan\u003eFor cells (2–5 mL), it is recommended to use a 15 mL multi-position magnetic stirrer.\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) The total adsorption volume is 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. Throughout the entire process, carefully control the temperature: keep the sorting buffer pre-cooled at all times, and proceed immediately with subsequent experiments after sorting. For flow cytometry, perform staining at 2–8°C in the dark, avoiding prolonged exposure to room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42302133895243,"sku":"S0K0014-100T","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/42d2357bc7eb431983d554c62cdf8f84_d8f11dcc-25b3-44cc-8710-cf65d355a472.jpg?v=1789880442"},{"product_id":"cd3-nanobeads-kit-mouse-ruo-s0k0015","title":"CD3ε Nanobeads Kit, mouse (RUO)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eBiotinylated anti-mouse CD3ε monoclonal antibody and streptavidin-conjugated nanomagnetic beads, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: Suitable for a total cell count of 5×10⁸, allowing for up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: Suitable for a total cell count of 1×10⁹, allowing for up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eFirst, CD3ε+ cells are labeled with a CD3ε-biotin–conjugated primary antibody. Subsequently, the cells are magnetically labeled using biotin-conjugated nanomagnetic beads. The cell suspension is then placed in a separation column within a magnetic field. The CD3ε+ cells that have been labeled with the magnetic beads remain retained in the column, while the unlabeled cells flow through; thus, CD3ε+ cells are separated from the other cell populations.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eCD3ε is a transmembrane protein with a molecular weight of 20 kDa and serves as a subunit of the T-cell receptor (TCR) complex. Together with the γ and δ subunits of CD3, it plays a crucial role in the assembly and expression of the TCR complex. CD3 is expressed on the surface of thymocytes, mature T lymphocytes, and natural killer T cells (NKT cells). Using the CD3ε nanosphere kit, mouse T lymphocytes and NKT cells can be depleted from various tissues, including the spleen, thymus, and lymph nodes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1mL","offer_id":42302138810443,"sku":"S0K0015-1mL","price":335.0,"currency_code":"USD","in_stock":true},{"title":"2mL","offer_id":42302138843211,"sku":"S0K0015-2mL","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ffa06f046bae49d681275dde28f550af.jpg?v=1788184921"},{"product_id":"mouse-fcr-blocking-reagent-s0b0599","title":"Mouse FcR Blocking Reagent","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eanti-mouse CD16\/CD32\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eLocation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMembrane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eClone Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2.4G2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eIsotype\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIgG2b,κ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eICC, FCM, Fc receptor blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.5mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS pH7.4, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFc receptor blocking\u003c\/td\u003e\n\u003ctd\u003e1.0ug (2μl\/test) per 10^6 cells in 100ul volume\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis antibody recognizes the same epitope as clone 2.4G2.All of the Fcγ receptors (FcγR) belong to the immunoglobulin superfamily and are the most important Fc receptors for inducing phagocytosis of opsonized (marked) microbes. This family includes several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which differ in their antibody affinities due to their different molecular structure.CD16\/CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42332802318411,"sku":"S0B0599-100T","price":65.0,"currency_code":"USD","in_stock":true},{"title":"200T","offer_id":42332802351179,"sku":"S0B0599-200T","price":85.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332802383947,"sku":"S0B0599-500T","price":190.0,"currency_code":"USD","in_stock":true},{"title":"25T","offer_id":43070816616523,"sku":"S0B0599-25T","price":30.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43070816649291,"sku":"S0B0599-50T","price":45.0,"currency_code":"USD","in_stock":true},{"title":"1000T","offer_id":42332802416715,"sku":"S0B0599-1000T","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b0c23adf31c848f69f9b7997782b5e88_120302a4-b051-457a-b789-2dc1176b04e2.jpg?v=1789585349"},{"product_id":"human-nave-cd4-t-cell-isolation-kit-column-free-s0k3014","title":"Human Naïve CD4 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Naïve CD4 T Cell Biotin-Antibody Cocktail, PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads, PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-human naïve CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells are marked by the antibody‑bead complex, while the target human naïve CD4 T cells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the magnetic field of the sorter. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled human naïve CD4 T cells remain in the supernatant. This supernatant constitutes the enriched fraction of human naïve CD4 T cells after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their unmanipulated, label-free state are isolated from human PBMCs and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe immunophenotype of human naïve CD4 T cells is CD3⁺CD4⁺CD45RA⁺CD45RO⁻. They constitute the core precursor population of helper T cells in the human adaptive immune system, responsible for initial antigen recognition and directed differentiation, and are also a key cell subset for maintaining immune homeostasis, mediating specific responses, and establishing immunological memory. The human naïve CD4 T-cell negative-selection kit is developed based on immuno‑magnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive selection. By specifically depleting non‑target cells—such as CD8 T cells, NK cells, B cells, monocytes, dendritic cells, granulocytes, and memory T cells—it yields unlabeled, native‑state, highly purified naïve CD4 T cells, perfectly preserving their initial quiescent state, surface molecule integrity, and multipotential differentiation capacity. This makes them well suited for various functional assays, including in vitro activation and proliferation, subtype‑directed differentiation induction, and cytokine secretion analysis, and they have become the mainstream research tool for isolating human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the sorting buffer; you will need to provide it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to sample volume; set aside a pre-sorting sample to determine the proportion of Human Naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10^7\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human Naïve CD4 T Cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin beads evenly;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 15 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 8 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor mixing in this step, you can use a pipette to gently blow the mixture 2–3 times;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can remix them again after about 2.5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 8 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling excessively;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 2.5 mL (in a 5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 7.5 mL (in a 15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic stand (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic stand (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe sorted cells can be centrifuged at 400 g for 7 minutes, for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan\u003e0.1-1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells (0.1-1 mL) are recommended to use a 5 mL single-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) with a total adsorption volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2-5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eFor cells (2–5 mL), it is recommended to use a 15 mL multi-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) The total adsorption volume is 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42917951438923,"sku":"S0K3014-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/779afb131f6942579be3b4556e5c4b8e.png?v=1788246176"},{"product_id":"mouse-neutrophil-isolation-kit-column-based-s0k1015","title":"Mouse Neutrophil Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Neutrophil Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection method to isolate Mouse Neutrophil cells from mouse bone marrow. In this negative selection process, non‑Mouse Neutrophil cells are labeled with biotin‑conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody complexes, while the target Mouse Neutrophil cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled Mouse Neutrophil cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of Mouse Neutrophil cells after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNeutrophils in their native, unmanipulated state are isolated from mouse bone marrow and peripheral blood and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe specific phenotype of mouse neutrophils is CD11b+Ly6G+. They are the most abundant innate immune cells in mouse peripheral blood and bone marrow, serving as the body's first line of defense against bacterial and fungal infections. They play a central role in physiological and pathological processes such as inflammatory responses, immune regulation, tumor microenvironment remodeling, and tissue damage repair. The mouse neutrophil negative-selection kit is developed based on immunomagnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive-selection methods. By specifically depleting non-target cells, it yields unlabeled, native-state, highly purified neutrophils, perfectly preserving their natural activity and physiological functions, and is well suited for various precise cell functional assays, making it the mainstream research tool for isolating mouse neutrophils today.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the sorting buffer; it must be provided separately. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column \u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect mouse bone marrow or peripheral blood, prepare a single-cell suspension, and perform cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eMouse bone marrow and peripheral blood must undergo red blood cell lysis using Red Blood Cell Lysis Buffer (without fixative, 1X) (Cat: S0D9002); it is recommended to count cells after staining with AO\/PI;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1 × 10^7 cells in 100 μL of MagSep Separation Buffer;\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; retain a pre-sorting sample to determine the proportion of mouse neutrophils;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse Neutrophil Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gently pipette up and down 2–3 times to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at 2–8°C for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at 2–8°C for 15 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at 2–8°C for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer equal to ten times the cell volume to wash the cells, centrifuge at 400g for 7 minutes, discard the supernatant, and resuspend to the original volume;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge at 400g for 7 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column selection magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells in 1 mL of MagSep Separation Buffer and load them into the separation column, collecting the target cells in a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer three times, collecting the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: 1. Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column has a maximum capacity of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42917951766603,"sku":"S0K1015-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5f8143674be5432db0535e4c6ec96ae8.jpg?v=1788858141"},{"product_id":"mouse-neutrophil-isolation-kit-column-free-s0k3015","title":"Mouse Neutrophil Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Neutrophil Biotin-Antibody Cocktail, PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads, PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non‑Mouse Neutrophil cells are labeled with biotin‑conjugated antibodies, and the cells are then incubated with streptavidin‑coated nanomagnetic beads. As a result, the non‑target cells become bound to the magnetic beads via the antibody complexes, while the target Mouse Neutrophil cells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the magnetic field of the sorter. Under the influence of the magnetic field, the magnetically labeled non‑target cells adhere to the tube wall, whereas the unlabeled Mouse Neutrophil cells remain in the supernatant. This supernatant constitutes the enriched fraction of Mouse Neutrophils after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNeutrophils in their native, unmanipulated state are isolated from mouse bone marrow and peripheral blood and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouse neutrophils (Mouse Neutrophil)'s specific phenotype isCD11b+Ly6G+Neutrophils are the most abundant innate immune cells in both peripheral blood and bone marrow, serving as the body's first line of defense against bacterial and fungal infections. They play a central role in a variety of physiological and pathological processes, including inflammatory responses, immune regulation, tumor microenvironment remodeling, and tissue repair. The mouse neutrophil negative-selection kit is developed based on immunomagnetic‑bead‑mediated negative selection, overcoming the labeling limitations inherent in positive-selection approaches. By specifically depleting non‑target cells, it yields unlabeled, native‑state, highly purified neutrophils that retain their intrinsic viability and physiological functions, making it ideally suited for a wide range of precise cell‑function assays and the current gold standard for neutrophil isolation in mice.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please prepare it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect mouse bone marrowor peripheral bloodand prepare a single-cell suspension,Perform cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eMouse peripheral blood requiresthe use ofred blood cell lysis bufferRed Blood Cell Lysis Buffer (without fixative, 1X)(cat:S0D9002) to perform red blood cell lysis;; It is recommended to stain cells withAO\/PIbefore counting;;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eUse100 μLMagSep Separation Bufferto resuspend1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; set aside a pre-sorting sample to determine the initialMouse Neutrophilratio; \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd10 μL MouseNeutrophilBiotin Antibody Cocktailto the sample, and gently mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle mixing can be achieved by pipetting up and down.2-3below;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the Cocktail with the cells.,2-8℃incubation10 min;2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-8℃ incubation10 min5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eVortexfor 5–30 secondsto mix wellStreptavidin beads;mix for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6add\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eto the sample;15 μL\/100 μL7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egently mix the magnetic beads and cells,\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-8℃incubate15min5minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gentle mixing can be achieved by gently pipetting up and down2-3times;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn this step, the magnetic beads may settle at the bottom; you can gently tap the tube twice to remix them;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2-8℃incubation15minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eaddMagSep Separation BufferAdd to the sample and immediately place it on the magnetic pole to prevent the magnetic beads from settling at the bottom in large quantities;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncrease the total volume to2.5 mL(5 mLflow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncrease the total volume to7.5 mL(15 mLcentrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic pole so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic rack (3min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic rack (5min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic rack and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote:The sorted cells can be centrifuged at400gfor7 min, for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan\u003e0.1-1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells(0.1-1mL) are recommended to use5 mLsingle-hole magnetic pole(\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e)the total adsorption volume is2.5mL; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2-5 ×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells(2-5mL) are recommended to use15 mLTandem magnetic poles(\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e)The total adsorption volume is7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2.If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42917951799371,"sku":"S0K3015-100T","price":920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8773c2e6276840909f963e39fe40db0b.jpg?v=1788246180"},{"product_id":"t-cell-activationexpansion-kit-mouseruo-s0k1016","title":"T Cell Activation\/Expansion Kit, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL Streptavidin NanoBeads, in a PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.4 mL CD3ε-Biotin, mouse (100 µg\/mL), in a PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.4 mL CD28-Biotin, mouse (100 µg\/mL), in a PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCan activate 2 × 10⁸ T cells;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003eThis kit is used for the in vitro activation and expansion of mouse T cells, with its core components consisting of streptavidin magnetic beads and biotin‑labeled mouse CD3ε\/CD28 antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter biotinylated CD3ε and CD28 antibodies are conjugated to the surface of streptavidin‑nanomagnetic beads, the beads can mimic antigen‑presenting cells, stimulating the activation of single‑cell suspensions from the spleen or lymph nodes, or purified resting T cells; on days 6–8 of culture, they can be restimulated to achieve large‑scale T‑cell expansion.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eThis product is intended for research use only and is not to be used for clinical diagnosis or treatment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouseTLymphocytes are the core effector cells of the adaptive immune system, mainly divided intoCD4+helperTcells andCD8+cytotoxicTcells, playing a key role in anti-infectious immunity, tumor immunity, immune regulation, and the development of autoimmune diseases. Under physiological conditions, naïveTcells in the mouse spleen and lymph nodes are in a quiescent, dormant state, barelyproliferating,expressing low levels of functional molecules, and thus unable to directly conduct in vitro functional experiments. Therefore, controlledTcell activation and large-scale expansion in vitro are core foundational technologies for studying murine immune mechanisms, validating cell functions, and constructing immune models; this reagentbox employsartificially simulated antigen-presenting cell technology, using biotinylatedCD3εandCD28antibody-conjugated anti-biotin magnetic beads to precisely replicateTcell activation's dual signals in vitro, achieving highly efficient, non-specific activation and expansion of polyclonalTcells, suitable for total mouseTcells,CD4T,CD8 TMass amplification of cells. Magneticbeads serve assolid-phase carriers, capable of simultaneously enriching large quantities ofCD3εandCD28antibodies, stably binding to the corresponding receptors on the surface ofTcells, continuously and evenly providing the first and second signals, thereby avoiding the instability inherent in natural antigen presentation and addressingprimary mouseTcell issues of poor in vitro activation and weak proliferation specific to certain species, significantly enhancing activation efficiency and expansion multiples.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.\u003c\/span\u003e \u003cspan style=\"font-weight:bold\"\u003eExperimental reagents\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.1\u003c\/span\u003e \u003cspan\u003eBuffer:pH 7.2 PBSbuffer, with the addition of2 mM EDTA,2–8℃Pre-cooled; do not use buffers containing calcium or magnesium ions\/Culture medium;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e \u003cspan\u003eCell culture medium: RPMI 1640, with the addition of10%fetal bovine serum (FBS)+IL-2(final concentration30–100 IU\/mL);Optional addition of50-100μMβ-mercaptoethanol, to maintain cell viability under rapid proliferation conditions;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e \u003cspan style=\"font-weight:bold\"\u003eMagnetic bead conjugation (conjugatingCD3ε\/CD28antibodies)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe magnetic beads contain no preservatives; all aliquoting operations must be performed under sterile conditions; it is recommended to conjugate500 μLof magnetic beads at a time, and after conjugation, the beads2–8℃can be stably stored for3months without washing away unbound antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e \u003cspan\u003eTake2 mLof a sterile centrifuge tube, and add100μLCD3ε-Biotin + 100μLCD28-Biotin, mix thoroughly by pipetting; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e \u003cspan\u003eadd300μL buffer (pH 7.2 PBScontaining2 mM EDTA) and mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3\u003c\/span\u003e \u003cspan\u003ethoroughly vortexto resuspend the magnetic beads, ensuring uniform dispersion of the beads; then pipette500μLStreptavidinNanoBeadsadd to the above antibody mixture, bringing the total volumeto 1 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4\u003c\/span\u003e \u003cspan\u003eplace the centrifuge tube on a rotator,at 2–8℃and incubate with gentle continuous rotationfor 1 hour; this results in coupledCD3ε\/CD28magnetic beads, which can be used directly afterward; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e3. Experimental steps\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAll operations must be performed in a sterile environment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.1\u003c\/span\u003e \u003cspan\u003eSample preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eResting mouse T cells derived from the spleenTCell activation and expansion, preparing single-cell suspensions from lymphoid organs (spleen, lymph nodes). Magnetic bead sorting can be used.TCell activation and expansion: positive selectionCD3\/CD4\/CD8 T cells:CD3ε Nanobeads Kit, mouse (RUO)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(Cat. No. S0K0015);CD4 Nanobeads,Mouse(RUO) (Cat. No. S0K0003);CD8 Nanobeads, Mouse(RUO)(Cat. No. S0K0005);negative selec-tionCD3\/ CD4\/CD8T cells::Mouse T Cell Isolation Kit(Cat. No. S0K1008\/S0K3006);Mouse CD4 T Cell Isolation Kit(Cat. No. S0K1005\/S0K3003);\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eMouse CD8 T Cell Isolation Kit (Cat. No. S0K1007\/S0K3004).\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRefer to the corresponding product manual for specific operations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2TCell activation and expansion procedures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe basic system in this protocol is suitable for1×10⁶cellsafter sortingTcellscorresponding to20μLof coupled magnetic beads; when the cell quantity doubles, all reagent volumes are scaled up accordingly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e3.2.1\u003c\/span\u003e \u003cspan style=\"color:#000000\"\u003eTake the sorted cells at a concentration of1×10⁶\/mLand resuspend them infresh complete culture medium (RPMI1640 + 10% FBS + 30–100 IU\/mL IL-2)in,take1mLof cellsinto a centrifuge tube,thoroughly mix the coupled magnetic beads, then add20μLof the coupledmagnetic beads,centrifuge at400 gfor7 min;(negative control: use uncoupled blank magnetic beads instead ofcoupledmagnetic beads;)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.2\u003c\/span\u003e \u003cspan\u003eAfter centrifugation, discard the supernatant and resuspend the pellet in1 mLFresh complete medium (RPMI1640 + 10% FBS + 30–100 IU\/mL IL-2),Add the cells to the corresponding plates for culture. To ensure optimal stimulation and proliferation, it is recommended to use a standard seeding density:1×10⁶cells\/cm²;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eOptimal Culture System Ratio Table for Each Culture Plate\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eCulture Plate Model\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eGrowth Base Area\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTotal CultureVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTCell Seeding Quantity\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eAmount of Coupled Magnetic Beads\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e96Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.31 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.25×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e48Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e24Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e12Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e80μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.3\u003c\/span\u003e \u003cspan\u003ePlace the culture plate ina CO₂incubator for cultivation, observe cell status daily, and replenish the medium promptly when it becomes insufficient;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.4\u003c\/span\u003e \u003cspan\u003eAfter culturing for2days, gently disperse cell clumps; at this step, samples can be taken for flow cytometry analysis.mouse CD25, mouse CD69to assess cell activation status.;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.5\u003c\/span\u003e \u003cspan\u003ePassage and expansion: every2days, subculture at a ratio of1:2or1:4proportion, add fresh culture medium, and if proliferation is too rapid, shorten the passage interval.;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.6\u003c\/span\u003e \u003cspan\u003eSecondary stimulation: after activating the culture for6–8days,Tcells enter a quiescentstate,if continued expansion is needed, magnetic beads must be added again to restimulate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3\u003c\/span\u003e \u003cspan\u003eMagnetic bead removal procedure\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIf downstream operations require magnetic bead sorting, replacement of stimulating antigens, or stimulants, the magnetic beads must be removed in advance:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.1\u003c\/span\u003e \u003cspan\u003eCollect the cells and transfer them to5\/15\/50 mLCentrifuge the tube and wash once with buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.2\u003c\/span\u003e \u003cspan\u003eResuspend the cells in buffer, at a maximum concentration2×10⁷cells\/mL, and pipette thoroughly to mix evenly;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.3\u003c\/span\u003e \u003cspan\u003eFix the centrifuge tube on the magnetic separator and let it stand for adsorption10 min;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e3.3.4\u003c\/span\u003e \u003cspan\u003eTransfer the cells to a new centrifuge tube, collect the bead-free cells, and proceed with subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"200T","offer_id":42920108884043,"sku":"S0K1016-200T","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e3cd042cbb2b4a40b4f93ff200030650.jpg?v=1788249620"},{"product_id":"monkey-th1th2inflammation-7-plex-kit-flow-cytometry-multiplex-bead-assay-s0q1002","title":"Monkey Th1\/Th2\/Inflammation 7-Plex Kit (Flow Cytometry Multiplex Bead Assay)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-2、IL-4、IL-5、IL-6、IL-10、IFN-γ、MCP-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: ＜10%；\u003cbr\u003eInter-assay: ＜15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerum; Plasma; Cell culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-10＜90 pg\/mL, others＜2.50 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70% - 130%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMonkey\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe Cytometric Bead Array (CBA) technology enables the capture of soluble analytes or analyte panels using beads with known sizes and fluorescence intensities, thereby allowing the detection of analytes via flow cytometry.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach capture bead in the CBA kit is conjugated with a specific antibody. The detection reagent provided with the kit is Streptavidin-Phycoerythrin, and the intensity of the fluorescent signal generated by this reagent is proportional to the amount of bound analyte.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eWhen the capture beads and detection reagent are incubated together with the test sample containing target analytes, sandwich complexes are formed. Detection of these complexes by flow cytometry allows for the identification of particles exhibiting the fluorescence characteristics of both the beads and the detection reagent.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":42927555838027,"sku":"S0Q1002-1x96T","price":2000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/919621f7b82e44c099c74b405591899a.jpg?v=1789560009"},{"product_id":"cd19-nanobeads-mouseruo-s0k0024","title":"CD19 Nanobeads, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD19 monoclonal antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for a total cell count of 5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 50 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for a total cell count of 1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eand can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCD19 Nanobeads, mouse are used to magnetically label CD19⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD19⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD19⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD19⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIsolate or deplete CD19+ cells from a single-cell suspension of mouse spleen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouse CD19 is encoded by the Cd19 gene and is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, serving as a classic marker of the B-cell lineage. This molecule begins to be expressed at the pro-B-cell stage in the bone marrow and remains on the surface of the vast majority of mature B cells, while its expression is downregulated in terminally differentiated plasma cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":42941098721355,"sku":"S0K0024-1 mL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":42941098754123,"sku":"S0K0024-2 mL","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b7442e19cfcf48c5b74a7c68b921413f.jpg?v=1788249817"},{"product_id":"human-il-2-single-plex-kit-flow-cytometry-bead-assay-s0q3001","title":"Human IL-2 Single Plex Kit (Flow Cytometry Bead Assay)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: 3%；\u003cbr\u003eInter-assay: 5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerum; Plasma; Cell culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.11 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1.28 pg\/mL-5250 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePlasma: 110%\u003cbr\u003eSerum: 101%\u003cbr\u003eCell culture supernatant: 117%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eInterleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against \"self-antigens\", which could result in \"autoimmunity\". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":42955075125323,"sku":"S0Q3001-1x96T","price":335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9e49acec619a46768f5f0fecc56a2543.jpg?v=1789556556"},{"product_id":"human-il-4-single-plex-kit-flow-cytometry-bead-assay-s0q3047","title":"Human IL-4 Single Plex Kit (Flow Cytometry Bead Assay)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: 3%；\u003cbr\u003eInter-assay: 5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerum; Plasma; Cell culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.1 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.43 pg\/mL-3500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePlasma: 110%\u003cbr\u003eSerum: 106%\u003cbr\u003eCell culture supernatant: 122%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eInterleukin 4 (IL-4) was first identified as a helper T cell product with the capacity to co-stimulate B cell growth in vitro. IL-4 also can rescue B-cells from apoptosis, enhancing their survival, and is responsible for immunoglobulin isotype switching to IgG1 and IgE. The effect of IL-4 signaling is mediated through the IL-4 receptor alpha chain (IL-4Rα). Upon binding to its ligand, IL-4Rα dimerizes either with the common gamma chain (γc) to produce the type-1 signaling complex located mainly on hematopoietic cells, or with the IL-13 receptor alpha 1 (IL-13Rα1) to produce the type-2 complex, which is expressed also on non-hematopoietic cells. The type-1 signaling complex is critical for Th2-skewing of T cells and the development of alternatively activated macrophages (AAMΦs), while the type-2 complex plays a role in non-hematopoietic responses to IL-4 and IL-13.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":42955075256395,"sku":"S0Q3047-1x96T","price":335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/83d982c52dd548cdbbe481e79e2621e4.jpg?v=1789556557"},{"product_id":"human-il-5-single-plex-kit-flow-cytometry-bead-assay-s0q3009","title":"Human IL-5 Single Plex Kit (Flow Cytometry Bead Assay)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: 3%；\u003cbr\u003eInter-assay: 5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerum; Plasma; Cell culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.34 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1.07 pg\/mL-8750 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePlasma: 108%\u003cbr\u003eSerum: 94%\u003cbr\u003eCell culture supernatant: 113%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eIL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":42955075387467,"sku":"S0Q3009-1x96T","price":335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e77edc2af2c64afc82fd1169e64bf3fa.jpg?v=1789556559"}],"url":"https:\/\/www.antbioinc.com\/collections\/flow-cytometry.oembed?page=10","provider":"AntBio","version":"1.0","type":"link"}