{"title":"Enzyme","description":null,"products":[{"product_id":"enterokinase-bovine","title":"Enterokinase, Bovine","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBovine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003erBoEnterokinase; Enteropeptidase; ENTK; PRSS7,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e28 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Yong S Z, Yong C J, Yuan C X, et al. Secretory Expression, Purification and Characterization of Bovine Enterokinase Light Chain[J]. Journal of Nanjing University (Natural Sciences), 2004. \u003cbr\u003e2.Haidong, Tan, and, et al. Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli[J]. Protein Expression \u0026amp; Purification, 2007. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eEnterokinase (enteropeptidase, EC 3.4.4.8) occupies a key position in the utilization of dietary proteins. The enzyme initiates intraluminar digestion of proteins by the proteolytic conversion of trypsinogen to trypsin, which in turn activates the other pancreatic zymogens (Kunitz, 1939a,b; Hadorn et al., 1969). The proteolytic attack of enterokinase is directed exclusively toward the Lys6-Ile7 peptide bond of trypsinogen leaving all other lysine and arginine bonds in the molecule unaffected (Maroux et al., 1971). The resultant cleavage produces the simultaneous release of active trypsin and of the \u003cbr\u003eamino-terminal hexapeptide Val-(Asp)d-Lys (Rovery et al., 1953; Davie and Neurath, 1955). This unique specificity exhibited by enterokinase is of interest as it relates to both the molecular basis of substrate recognition and the control of the digestive process. \u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e5 U\/μL Enterokinase, Bovine in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eOptimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:\u003cbr\u003e1. Combine 500 ug of sample with reaction buffer\u003cbr\u003e* Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)\u003cbr\u003e2. Add 1 U of Enterokinase light chain\u003cbr\u003e3. Incubate at 25°C for 16 hours\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500U","offer_id":41518983577675,"sku":"UA070001-500U","price":185.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41518983610443,"sku":"UA070001-1000U","price":330.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/50f0701767d54bdf85e9ce93f33f23a9.png?v=1789380012"},{"product_id":"hyaluronidase-ph20spam1-human","title":"Hyaluronidase PH20\/SPAM1, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLeu36-Tyr482\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e60-70kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20mM PB, 150mM NaCl, pH7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· 6 months, -20 to -70 °C under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eHyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). There are six known types (hyaluronidase 1–4, PH-20, and HYALP1). Human hyaluronidase is present both in organs (testis, spleen, skin, eyes, liver, kidneys, uterus, and placenta) and body fluids (tears, blood, and semen). Testicular PH20 hyaluronidase is found on the surface of human sperm and inner acrosomal membrane and degrades hyaluronic acid in the ovum during fertilization. Hyaluronidase PH20 is known as sperm adhesion molecule 1 (SPAM1) and random hydrolysis of (1, 4)-linkages between N-acetyl- beta-D-glucosamine and D-glucuronate residues in hyaluronic acid (HA). PH20\/SPAM1 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. PH20\/SPAM1 protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":41293285064779,"sku":"UA070006-25μg","price":200.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41293285032011,"sku":"UA070006-100μg","price":750.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":41518894481483,"sku":"UA070006-500μg","price":2670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b681d67eff664f09b5bdad15afae5adc.png?v=1787648565"},{"product_id":"pngase-f","title":"PNGase F","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eElizabethkingia miricola\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePNGase F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePeptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e35.7kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% Glycerol, pH7.5\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.  Frank Maley and Robert B. Trimble and Anthony L. Tarentino and Thomas H. Plummer Jr. Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases[J]. Analytical Biochemistry, 1989.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003ePeptide: N-glycosidase F (PNGase F) is an asparagine amidase produced by Flavobacterium meningosept-icum that serves as a useful tool in the research on protein N-glycosylation. Recombinant expression in E.coli. The cleavage site of PNGase F is the amide bond between N-acetylglucosamine (GlcNAc) and aspartate residues on the medial side of the glycoprotein, and converts aspartyl to aspartic acid on the enzymolysis protein. This product is often used for complete deglycosylation of antibodies and their associated proteins.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins. The kit includes recombinant Peptide N-Glycosidase F(PNGase F) enzyme, which cleaves N-glycan chains at the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides, and a 10X reaction buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 484.595px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 262.863px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eComponents                         \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 220.991px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eAmount\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003ePNGase F*                            \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e100000U\/mL\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eReaction Buffer (10×)           \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e200mM Tris, PH 7.5  25°C\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eDenaturing Buffer(10×)        \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e5% SDS、400 mM DTT\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003eNP-40(10×)                         \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 595.532px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:11.0000pt\"\u003e\u003cfont\u003e10% NP-40 in MilliQ-H2O\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e*\u003c\/span\u003e One unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 10 μ\u003cspan\u003eg of denatured RNase B in 1 hour at 37\u003c\/span\u003e°\u003cspan\u003eC in a total reaction volume of 10 \u003c\/span\u003eμl\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"text-align: inherit; font-family: var(--body-font-family); color: rgb(33, 37, 41); font-weight: bold; font-size: 10pt;\"\u003eI. \u003c\/span\u003e\u003cb style=\"text-align: inherit; color: var(--body-color); font-family: var(--body-font-family); font-size: var(--body-font-size);\"\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003eDenaturing Reaction Conditions:\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e1. Combine 1-20 µg of glycoprotein, 1 µl of Denaturing Buffer (10X) and H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO (if necessary) to make a 10 µl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e2. Denaturation is terminated by heating to 100\u003cfont\u003e℃\u003c\/font\u003e for 10-20min and cooling to room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e3. Make a total reaction volume of 20 µl by adding 2 µl Reaction Buffer (10×), 2 µl 10% NP-40 and 6 µl H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e4. Add 1 µl PNGase F, mix gently.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e5. Enzymatic digestion at 37\u003cfont\u003e℃\u003c\/font\u003e for 1h \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e6. Analyze by the method of SDS-PAGE\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003eII. \u003c\/span\u003e\u003cb\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003eNon-Denaturing Reaction Conditions:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e1. Combine 1-20 µg of glycoprotein, 2 µl of Reaction Buffer (10×) and H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO (if necessary) to make a 20 µl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e2. Add 2-5 µl PNGase F, mix gently.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e3. Enzymatic digestion at 37°C for 4 - 24 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e4. Analyze by the method of SDS-PAGE\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.The target protein should be in a solution compatible with Rapid PNGase F activity. Avoid buffers containing SDS, as it inhibits PNGase F. Common stabilizing reagents such as Tween, Triton X-100, NP-40, octyl glucoside and non-detergent sulfobetaine, as well as traces of organic solvents, can prevent optimal rapid deglycosylation. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.N-linked glycans containing core α1-3 Fucose are not cleaved by PNGase F.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.For deglycosylation of native glycoprotiens, increased incubation time and increased amount of enzyme may be needed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Optimize reaction conditions for each substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.If a larger amount of glycoprotein is used, scale up reaction volumes accordingly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6.Although this product has been optimized for the rapid removal of N-glycans from antibodies, it can be utilized with various other glycoproteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of carbohydrate from 10μg denatured RNaseB at 37℃ for 1 hour in a 10μL reaction system.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"15000U","offer_id":41293285261387,"sku":"UA070014-15000U","price":170.0,"currency_code":"USD","in_stock":true},{"title":"75000U","offer_id":41293285294155,"sku":"UA070014-75000U","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9caf6fcdfc1e4c93862de4077d6ff810.jpg?v=1789113765"},{"product_id":"ides-protease","title":"IdeS Protease","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e IdeS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e37.7kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePBS pH 6.6, 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eN.A\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; \u003cbr\u003e\u003cbr\u003e1 week, 2 to 8 °C under sterile conditions; \u003cbr\u003e\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e1.Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549.\u003c\/p\u003e\n\u003cp\u003e2.Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615. \u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eIdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcuspyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003ca\u003e1.Add the desired amount of 5mg IgG in digestion buffer or other compatible \u003c\/a\u003ebuffer*.\u003c\/p\u003e\n\u003cp\u003e2.Add IdeS Protease to the reaction system:\u003c\/p\u003e\n\u003cp\u003e•Add 1 unit of IdeS Protease per 1µg of IgG to be digested.\u003c\/p\u003e\n\u003cp\u003e•For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.\u003c\/p\u003e\n\u003cp\u003e3.Incubate sample at 37°C for 30–60 minutes\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"2000U","offer_id":41293285589067,"sku":"UA070027-2000U","price":275.0,"currency_code":"USD","in_stock":true},{"title":"5000U","offer_id":41293285621835,"sku":"UA070027-5000U","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1b39289c-a100-4d9e-a7a0-a67af2f90826.png?v=1787648589"},{"product_id":"idez-protease","title":"IdeZ Protease","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIgG endopeptidase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e36kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eN.A\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; \u003cbr\u003e\u003cbr\u003e1 week, 2 to 8 °C under sterile conditions; \u003cbr\u003e\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e1.Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26. \u003c\/p\u003e\n\u003cp\u003e2.Cheng-Wei H, Jie P, Yu-Qing O, et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J]. Chinese Journal of Preventive Veterinary Medicine, 2018.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eIdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Addthe desired amount of 5mg IgG in digestion buffer or other compatible buffer*.\u003c\/p\u003e\n\u003cp\u003e2. AddIdeZ Protease to the reaction system:\u003c\/p\u003e\n\u003cp\u003e• Add 1 unit of IdeZ Protease per 1µg of IgG to be digested.\u003c\/p\u003e\n\u003cp\u003e• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µgof IgG.\u003c\/p\u003e\n\u003cp\u003e3. Incubate sample at 37°C for 30–60 minutes\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4000U","offer_id":41293285687371,"sku":"UA070028-4000U","price":385.0,"currency_code":"USD","in_stock":true},{"title":"40000U","offer_id":41293285654603,"sku":"UA070028-40000U","price":2135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4b2bcb0-3a32-4947-b6fe-2e4fb05a052c.png?v=1787648571"},{"product_id":"ides-protease-tag-free","title":"IdeS Protease (Tag Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIdeS、IdeS Protease、Immunoglubulin-degrading enzyme\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e38 kDa (\u003cfont\u003eR\u003c\/font\u003eeducing)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePBS pH 6.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e·\u003c\/font\u003e12 months from date of receipt, -20 to -70 °C as supplied. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e· It can be stored at 4-8℃ for one week after dissolution, under sterile conditions\u003c\/font\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e· \u003c\/font\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003ca\u003e[1]Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterizationof the streptococcal endopeptidase, IdeS, reveals that it is a cysteineprotease with strict specificity for IgG cleavage due to exositebinding.[J].Biochemistry, 2004, 43(49):15540-15549.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e[2] Pawel-Rammingen V, U. IdeS, a novelstreptococcal cysteine proteinase with unique specificity for immunoglobulinG[J]. Embo Journal, 2014, 21(7):1607-1615. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eIdeS Protease is a cysteine hydrolase secretedby the human pathogen Streptococcus pyogenes. This product use E. colirecombinant expression, high purity, has good enzyme digestion activity, canspecifically identify IgG, and perform enzyme digestion at specific sites inthe hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2fragments and Fc fragments, which can identify human and other animal IgG, suchas mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can beused for structural characterization analysis of antibodies and fusion proteindrugs\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003ca\u003e\u003cspan style=\"font-size:10.5pt\"\u003ePBS pH 6.6\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003ca\u003e1. Add the desired amount of 5mg IgG in digestionbuffer or other compatible\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003ebuffer\u003cspan style=\"font-size:10.5pt\"\u003e*\u003c\/span\u003e.\u003c\/p\u003e\n\u003cp\u003e2. Add IdeS Protease(Tag Free) tothe reaction system:\u003c\/p\u003e\n\u003cp\u003e•Add 1 unit of IdeS Protease(Tag Free) per 1µg of IgG to be digested.\u003c\/p\u003e\n\u003cp\u003e•For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.\u003c\/p\u003e\n\u003cp\u003e3. Incubate sample at 37°C for30–60 minutes\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"2000U","offer_id":41293285752907,"sku":"UA070032-2000U","price":310.0,"currency_code":"USD","in_stock":true},{"title":"5000U","offer_id":41293285785675,"sku":"UA070032-5000U","price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/08e376b2-bbaa-4fe1-9c76-e51800e936a8.png?v=1787630581"},{"product_id":"murine-rnase-inhibitor","title":"Murine RNase Inhibitor","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRibonuclease inhibitor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e50 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Wanhua, Guo, and et al.  High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners[J]. Protein Expression \u0026amp; Purification, 2011.\u003cbr\u003e[2] Chen, C. Z. , and  R. Shapiro . \"Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A. \" Proceedings of the National Academy of Sciences 94.5(1997):p. 1761-1766.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eMurine RNase inhibitor is expressed in soluble form in E. coli and capable of inhibiting a wide range of RNases (RNase A, B, C). Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatible with various commercial reverse transcriptase’s and various DNA polymerases. Compared with human RNase inhibitor, murine RNase inhibitor does not contain two cysteines that are very sensitive to oxidation, so it has higher antioxidant activity and is more suitable for high DTT sensitive experiments (such as qPCR).\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 40 U\/µL Murine RNase Inhibitor, 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAdd inhibitor to achieve final concentration of 1 U\/μL in your reaction.\u003cbr\u003eDuring assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"3KU","offer_id":41293286047819,"sku":"UA070037-3KU","price":35.0,"currency_code":"USD","in_stock":true},{"title":"15KU","offer_id":41293286015051,"sku":"UA070037-15KU","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/93cf2531136240dd831284e3f68289c7.png?v=1787623336"},{"product_id":"pngase-fglycerol-free","title":"PNGase F(Glycerol-free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eElizabethkingia miricola\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePeptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, Peptide N-Glycosidase F, PNGase F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e36kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e[1] FrankMaley and Robert B. Trimble and Anthony L. Tarentino and Thomas H. Plummer Jr. Characterizationof glycoproteins and their associated oligosaccharides through the use ofendoglycosidases[J]. Analytical Biochemistry, 1989.. \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e[2] B, Ling Hua A, et al. Highly efficient productionof peptides: N -glycosidase F for N -glycomics analysis[J]. Protein Expressionand Purification, 2014, 97(5):17-22. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003ePeptide:N-glycosidase F (PNGase F) is an asparagine amidase produced by Flavobacteriummeningosept-icum that serves as a useful tool in the research on proteinN-glycosylation. The cleavage site of PNGase F is the amide bond betweenN-acetylglucosamine (GlcNAc) and aspartate residues on the medial side of theglycoprotein, and converts aspartyl to aspartic acid on the enzymolysisprotein. This productoverexpressed in \u003ci\u003eE.coli\u003c\/i\u003eand used for complete deglycosylation of antibodies and their associatedproteins\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eStorageSolution\u003cspan style=\"color:black\"\u003e :\u003c\/span\u003e 40U\/ul PNGaseF、\u003cspan style=\"color:black\"\u003e20 mM Tris-HCl、50 mM NaCl, 5 mM EDTA(pH 7.5 @ 25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e10*NP-40:\u003c\/span\u003e 10% NP-40 inMilliQ-H2O\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e10*Denaturing Buffer: 5% SDS、400 mM DTT\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e10*Reaction Buffer: 500mMTris-HCl (pH 7.5 @ 25°C)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003e1. \u003c\/span\u003e\u003cb\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003eDenaturing Reaction Conditions:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e1. Combine 1-20 µg of glycoprotein, 1 µl of Denaturing Buffer (10X) and H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO (if necessary) to make a 10 µl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e2. Denaturation is terminated by heating to 100\u003cfont\u003e℃\u003c\/font\u003e for 10-20min and cooling to room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e3. Make a total reaction volume of 20 µl by adding 2 µl Reaction Buffer (10×), 2 µl 10% NP-40 and 6 µl H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e4. Add 1 µl PNGase F, mix gently.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e5. Enzymatic digestion at 37\u003cfont\u003e℃\u003c\/font\u003e for 1h \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e6. Analyze by the method of SDS-PAGE\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003e2. \u003c\/span\u003e\u003cb\u003e\u003cspan style=\"color:rgb(33,37,41);font-weight:bold;font-size:10.0000pt\"\u003eNon-Denaturing Reaction Conditions:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e1. Combine 1-20 µg of glycoprotein, 2 µl of Reaction Buffer (10×) and H\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:9.0000pt\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003eO (if necessary) to make a 20 µl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e2. Add 2-5 µl PNGase F, mix gently.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e3. Enzymatic digestion at 37°C for 4 - 24 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.0000pt\"\u003e4. Analyze by the method of SDS-PAGE\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of carbohydrate from 10μg denatured RNaseB at 37℃ for 1 hour in a 10μL reaction system.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"15KU","offer_id":41293286178891,"sku":"UA070041-15KU","price":170.0,"currency_code":"USD","in_stock":true},{"title":"75KU","offer_id":41293286211659,"sku":"UA070041-75KU","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/dc4915e7-ce9d-451e-bf87-62ee3ca25abd.jpg?v=1787623306"},{"product_id":"t4-dna-ligase","title":"T4 DNA Ligase Ⅱ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA Ligase、Polydeoxyribonucleotide synthase [ATP]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e81 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; SEC-HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol（pH 7.4 @ 25°C）\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 1 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Williamson A, Pedersen H. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida. Protein Expr Purif. 2014 May; 97:29-36. \u003cbr\u003e[2] Liu X, Huang A, Luo D, Liu H, Han H, Xu Y, Liang P. Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli. Protein Expr Purif. 2015 May;109:79-84. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eT4 DNA ligase is a type of DNA ligase. DNA ligase catalyzes the formation of phosphodiester bonds at single-stranded DNA breaks in double-stranded DNA in vivo. DNA ligase has important biological functions in organisms. In DNA repair and recombination, DNA ligase plays a role in connecting gaps. In the process of DNA replication, the synthesis of the lagging strand is discontinuous, and DNA ligase connects the discontinuous DNA strand into a continuous DNA strand. T4 DNA Ligase Ⅱ is a recombinant protein fusion of T4 DNA Ligase and adenylate kinase, The purified T4 DNA Ligase Ⅱ not only is fully active for DNA ligation, but also can use ADP in addition to ATP as energy source since adenylate kinase converts ADP to ATP and AMP. It does not contain DNA endonuclease, exonuclease and phosphatase, and does not contain RNA enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 400U\/μl T4 DNA Ligase、10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol (pH 7.4 @ 25°C)10*Reaction Buffer: 500 mM Tris-HCl、100 mM MgCl2、10 mM ATP、100 mM DTT (pH 7.5 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. Set up the following reaction in a microcentrifuge tube on ice. Add the following components in sequence. Note that the table shows a ligation using a molar ratio of 1:5 vector to insert for the indicated DNA sizes.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume 20μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10* T4 DNA Ligase Buffer \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVector DNA (such as \u003cfont\u003ep\u003c\/font\u003eET-28a 5369\u003cfont\u003ebp\u003c\/font\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e50ng \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eInsert DNA (530bp)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e25ng \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNuclease-free water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eUp to 20 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eT4 DNA Ligase Ⅱ\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e2. Gently mix the reaction through the up and down pipette, and inhale the liquid briefly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e3. For sticky ends, incubate at 16°C overnight or at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e4. For blunt ends or single base overhangs, incubate overnight at 16°C or room temperature for 2 hours (alternatively, high concentrations of T4DNA ligase can be used for 10 minutes of ligations).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e5. Chill on ice and transform 1-5 μl of the reaction into competent cells.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1.ATP is an essential cofactor in the reaction. This is in contrast to E. coli DNA Ligase, which requires NAD as a cofactor. \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2.If T4 DNA Ligase is to be diluted, it is recommended that it be diluted with 50% glycerol in storage buffer and stored at -20°C. 3. Room temperature ligation\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme required to ligate 50% of the HindIII-digested λ DNA fragments [DNA 5´-end concentration of 0.12 µM (300 μg\/ml)] within 30 minutes at 16°C in a 20 µl reaction system and in 1X T4 DNA Ligase Reaction Buffer.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"20KU","offer_id":41293286309963,"sku":"UA070048-20KU","price":35.0,"currency_code":"USD","in_stock":true},{"title":"100KU","offer_id":41293286277195,"sku":"UA070048-100KU","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/537263e9e286478d8999571b8409c78f.png?v=1787688107"},{"product_id":"rnase-r","title":"RNase R","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRibonuclease R、RNase R\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e94 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v\/v) Glycerol, 0.1% (w\/v) Triton X-100 (pH7.5 @25℃)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 1 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Cheng, Z.-F. Purification and Characterization of the Escherichia coli Exoribonuclease RNase R COMPARISON WITH RNase II[J]. Journal of Biological Chemistry, 2002, 277(24):21624.  \u003cbr\u003e[2] Cheng Z F, Deutscher M P. An Important Role for RNase R in mRNA Decay[J].Molecular Cell, 2005, 17(2):313-318.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eRibonuclease R (RNase R) is a Mg2+ dependent 3'→5' exonuclease derived from Escherichia coli. RNase R can digest all linear RNA. However, it cannot digest ring-shaped RNA, lasso structure or double-stranded RNA molecules with 3 'end protruding ends less than 7 nucleotides, tRNA and 5SRNA with complex secondary structure. RNase R is commonly used in gene expression and variable shear studies and can digest linear RNA to enrich circular RNA or lasso structured RNA. This product does not contain DNase, other RNA endonuclease and exonuclease activities.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 20U\/μl RNase R, 50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v\/v) Glycerol, 0.1% (w\/v) Triton X-100 (pH7.5 @25℃)10*Reaction Buffer: 200mM Tris-HCl, 1M KCl, 1mM MgCl2 (pH8.0 @25℃)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSet up the following reaction in a microcentrifuge tube on ice. Add the following components in sequence. 2）Reaction at 37℃ for 10 min-30 min\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. The activity of RNase R requires 0.1-1.0 mM Mg2+; \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. With the increase of substrate RNA, digestion time and enzyme amount can be appropriately prolonged\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit converts 1μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37℃ in 20mM Tris-HCl (pH8.0), 100mM KCl and 0.1mM MgCl2\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41293286473803,"sku":"UA070049-250U","price":70.0,"currency_code":"USD","in_stock":true},{"title":"2500U","offer_id":41293286441035,"sku":"UA070049-2500U","price":435.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b4099439dc094179a9425a389b64883b.png?v=1787688106"},{"product_id":"endo-s2","title":"Endo-glycosidase S2","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEndo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e93 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl、50 mM NaCl、pH 7.5 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Sjögren Jonathan, et al. \"EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans.\" Glycobiology 10:1053-1063.\u003cbr\u003e[2] Jonathan Sjögren, et al. \"EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein. \" Biochemical Journal 455.Pt 1(2013).\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eEndo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eStorage Solution : 200U\/ul Endo S2 、20 mM Tris-HCl, 50 mM NaCl. (pH 7.5 @ 25°C) \u003c\/p\u003e\n\u003cp\u003e10*Reaction Buffer： 500 mM sodium acetate (pH 6.0 @ 25°C)\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume. \u003c\/p\u003e\n\u003cp\u003e2. Add 1 µl EndoS2. \u003c\/p\u003e\n\u003cp\u003e3. Incubate reaction at 37°C for 1 hour\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"6KU","offer_id":41293286539339,"sku":"UA070055-6KU","price":480.0,"currency_code":"USD","in_stock":true},{"title":"30KU","offer_id":41293286506571,"sku":"UA070055-30KU","price":1920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5a08778a09244156ab4a5fd6b738098d.png?v=1787688221"},{"product_id":"bst-dna-polymerase-full-length","title":"Bst DNA Polymerase, Full length","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA polymerase I\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e100kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Genetic Analysis: Biomolecular Engineering, 1996, 12(5-6):185-195.\u003cbr\u003e2. BioTechniques, 1991, 11(1):76-8, 80, 82-7.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eBst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity. It can be used to isothermal DNA amplification and Primer extension.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C\u003cbr\u003e10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、100 mM KCl、20mM MgSO4、0.1% Tween® 20、pH 8.8@25°C\u003cbr\u003eMagnesium Sulfate (MgSO4) Solution：100mM MgSO4\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003eIncubate the following reaction at 65°C for 30–60 minutes\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 484.653px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 263.921px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eComponent\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 219.991px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10X Isothermal Amplification Buffer II\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1X (contains 2 mM MgSO4)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eMgSO4 (100 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e6 mM (8 mM total)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edNTP Mix (10 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.4 mM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFIP\/BIP Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.6 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eF3\/B3 Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.2 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eLoopF\/B Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.4 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003ci\u003e\u003cspan style=\"font-style:italic;font-size:10.5000pt\"\u003eBst\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e  DNA Polymerase (8,000 U\/ml)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e320 U\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDNA or RNA Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u0026gt; 10 copies or more\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNuclease-free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eto 25 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTotal Reaction Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eBst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eReaction temperatures above 70°C are not recommended.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eCannot be used for thermal cycle sequencing or PCR.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500U","offer_id":41293286735947,"sku":"UA070064-500U","price":85.0,"currency_code":"USD","in_stock":true},{"title":"5000U","offer_id":41293286703179,"sku":"UA070064-5000U","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/664351b332b4483d9f9d201d7f43a9ce.jpg?v=1789110082"},{"product_id":"bst-dna-polymerase-large-fragment","title":"Bst DNA Polymerase, Large fragment","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA polymerase I\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e67kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTags \u0026amp; Cleavage sites\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Nucleic Acids Research, 2000, 28(12):E63.\u003cbr\u003e2. Chemical Communications, 2014, 50(28):3747-3749.\u003cbr\u003e3. Current Protocols in Molecular Biology, 2014(Suppl.105):15.14.1-15.14.14.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eBst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the\u003cbr\u003e5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. It can be use to isothermal amplification (LAMP), DNA sequencing through high GC regions and Rapid Sequencing from nanogram amounts of DNA template.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C\u003cbr\u003e10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、20mM MgSO4、100 mM KCl、1% Tween® 20、pH 8.8@25°C\u003cbr\u003eMagnesium Sulfate (MgSO4) Solution：100mM MgSO4\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003eIncubate the following reaction at 65°C for 30–60 minutes\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 527.662px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 273.921px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eComponent\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 253.009px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10X Isothermal Amplification Buffer II\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1X (contains 2 mM MgSO4)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eMgSO4 (100 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e6 mM (8 mM total)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edNTP Mix (10 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.4 mM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFIP\/BIP Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.6 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eF3\/B3 Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.2 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eLoopF\/B Primers (25X)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.4 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003ci\u003e\u003cspan style=\"font-style:italic;font-size:10.5000pt\"\u003eBst\u003c\/span\u003e\u003c\/i\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e  DNA Polymerase (8,000 U\/ml)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e320 U\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDNA or RNA Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u0026gt; 10 copies or more\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNuclease-free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eto 25 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTotal Reaction Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1.Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2.100 µg\/ml BSA or 0.1%Triton X-100 is required for long term storage.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3.Reaction temperatures above 70°C are not recommended.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e4.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"800U","offer_id":41293286834251,"sku":"UA070065-800U","price":40.0,"currency_code":"USD","in_stock":true},{"title":"8000U","offer_id":41293286801483,"sku":"UA070065-8000U","price":335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/19bcad011577439bba82304dbae6fff4.jpg?v=1787911384"},{"product_id":"endo-h","title":"Endo H","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptomyces picatus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEndo-beta-N-acetylglucosaminidase H,Endoglycosidase H,Endo H\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e30kD (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e[1] Wang F , Wang X , Yu X ,et al. High-LevelExpression of Endo-β-N-AcetylglucosaminidaseH from Streptomyces plicatus in Pichia pastoris and Its Application for theDeglycosylation of Glycoproteins[J].Plos One, 2015, 10.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003c\/span\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e[2] Maley F, Trimble R B,Tarentino A L,et al. Characterization of glycoproteins and their associatedoligosaccharides through the use of endoglycosidases.[J]. Analytical Biochemistry,1989,180(2):195-204.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003eEndo H is a recombinant glycosidase cloned fromStreptomyces plicatus and overexpressed in\u003ci\u003e E.coli\u003c\/i\u003e. It cleaves thechitobiose core of high-mannose oligosaccharides and a limited number of hybridoligosaccharides from asparagine-linked glycoproteins, but not complex,oligosaccharides from glycoproteins.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eStorageSolution: 500U\/μL EndoH、20 mM Tris-HCl、50 mM NaCl、5 mM EDTA \u003c\/span\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e(pH 7.5@ 25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e10*Denaturing Buffer: 5% SDS、400 mM DTT\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003c\/span\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e10*Reaction Buffer:\u003c\/span\u003e\u003cspan style=\"font-size: 14px;\"\u003e \u003c\/span\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e500 mM sodiumacetate(pH 6 @ 25°C)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e1.Combine 1-20 μg of glycoprotein, 1 μl of 10*Denaturing Buffer and H20(if necessary) to make a 10 μl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e2.Denature glycoprotein by heating raection at 100°Cfor 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e3.Make a total reaction volume of 20 μlby adding 2 μl of \u003c\/span\u003e\u003cspan style=\"color: black; font-size: 14px;\"\u003e10*ReactionBuffer\u003c\/span\u003e\u003cspan style=\"font-size: 14px;\"\u003e, H20and 1-5 μl Endo H.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e4. Incubate reaction at 37°C for 1 hour.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10KU","offer_id":41518781759563,"sku":"UA070040-10KU","price":60.0,"currency_code":"USD","in_stock":true},{"title":"50KU","offer_id":41518781792331,"sku":"UA070040-50KU","price":225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/88b17f8e-0f51-4b83-a631-bdcd8a4dee69.png?v=1787623314"},{"product_id":"endo-s","title":"Endo S","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEndo-beta-N-acetylglucosaminidase EndoS, Endoglycosidase S, EndoS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e109  kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e[1]Collin,M.EndoS, a novel secreted protein from Streptococcus pyogenes withendoglycosidase activity on human IgG[J].Embo Journal, 2014,20(12):3046-3055. \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e[2] Beatriz,Trastoy,Joseph,et al.Liquid–liquiddiffusion crystallization improves the X-ray diffraction of EndoS, anendo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity onhuman IgG[J].Acta Crystallographica, 2013.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eAnenzyme expressed by Streptococcus pyogenes capable of releasing theterminal sialic acid residues from glycoproteins such as immunoglobulins.Specifically catalyzes the hydrolysis of the beta-1,4 linkage betweenthe first two N-acetylglucosamine residues of the complex-type N-linked glycanlocated on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 orIGHG4), thereby preventing interaction between IgGs and Fc receptors andability to activate the complement pathway. Endo S is an endoglycosidasespecific for cleaving the N-linked glycans from the chitobiose core of theheavy chain of native IgG.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eStorageSolution\u003cspan style=\"color:black\"\u003e :\u003c\/span\u003e 200U\/ul Endo S、\u003cspan style=\"color:black\"\u003e20mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. (pH 7.5 @ 25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e10*Reaction Buffer：50mM CaCl2、500 mM sodium acetate (pH 5.5 @ 25°C)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1.Combine 100 µg of native IgG, 1 µl of \u003cspan style=\"color:black\"\u003e10*Reaction Buffer\u003c\/span\u003e and H20 (if necessary) to make a 10 µl totalreaction volume.\u003c\/p\u003e\n\u003cp\u003e2.Add 1 µl Endo S.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e3. Incubate reaction at 37°C for 1 hour\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 5μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10µl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"6KU","offer_id":41518781988939,"sku":"UA070039-6KU","price":225.0,"currency_code":"USD","in_stock":true},{"title":"30KU","offer_id":41518782021707,"sku":"UA070039-30KU","price":675.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/50bf3a6a-4632-4057-863d-2a24752d386a.png?v=1787623323"},{"product_id":"pyrophosphatase-inorganic","title":"Pyrophosphatase, Inorganic","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eYeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eInorganic pyrophosphatase、PPase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.1U\/μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e33.5 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e[1] Lahti R , Pitk RantaT , Valve E ,et al. Cloning and characterization of the geneencoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal ofBacteriology, 1988, 170(12):5901-7\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e[2] Satoh T , Watanabe M ,Nogi S I ,et al. Molecular Cloning, Expression, and Site-DirectedMutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journalof Biochemistry, 1998, 124(1):79-88.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003ePyrophosphatase, Inorganic is a yeast derivedinorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is anenzyme that catalyzes the conversion of one molecule of pyrophosphate to twomolecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis toform phosphates. In molecular biology, it can be used to increase theproduction of RNA in vitro transcription reactions.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStorage Solution: 0.1U\/μl\u003cspan style=\"color:black\"\u003e Pyrophosphatase, Inorganic\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e、\u003c\/span\u003e\u003cspan style=\"font-size:10.5pt\"\u003e 20mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50%Glycerol \u003cspan style=\"color:black\"\u003e(pH 8.0 @ 25°C)\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eUse 1-3 units per mlin a high yield in vitro RNA synthesis reaction \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM PPi).\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10U","offer_id":41518782578763,"sku":"UA070038-10U","price":40.0,"currency_code":"USD","in_stock":true},{"title":"50U","offer_id":41518782611531,"sku":"UA070038-50U","price":80.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/65facccf-40f2-4510-a036-d41e372495fb.png?v=1787623336"},{"product_id":"dnase","title":"DNase Ⅰ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBovine Pancreatic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNASE,Deoxyribonuclease-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e72kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u0026amp;HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag, MBP Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e10 mM Tris-HCl, 2 mM CaCl2 ,50% Glycerol，(pH 7.6, 25°C)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e[1] Vanecko S, Laskowski M. Studies of theSpecificity of Deoxyribonuclease I[J]. Journal of Biological Chemistry, 1961,236(236):3312-6.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:black\"\u003e[2] Kienzle N, Young D, Zehntner S, et al. DNaseItreatment is a prerequisite for the amplification of cDNA from episomal-basedgenes[J]. Biotechniques, 1996, 20(4):612-6.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt;color:black\"\u003e[3] Michael,R, Green, etal. Human β-globin pre-mRNA synthesized in vitro is accurately spliced inxenopus oocyte nuclei[J].Cell, 1983, 32(3):681-694.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eDNase I(Deoxyribonuclease I), can digest single or double-stranded DNA to produce monodeoxynucleotides or single or double-stranded oligo deoxynucleotides, itsoptimal working pH range is 7-8. DNase I activity is dependent on Ca2+and can be activated by other bivalent metal ions such as Mg2+, Mn2+,Zn2+, etc. In the presence of Mg2+, the enzyme canrandomly recognize and cut any site on any strand of DNA. In the presence of Mn2+,two strands of DNA can be cut at the same site to form sticky ends with flatends or 1-2 nucleotides protruding.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eStorageSolution:  2 U\/ul DnaseⅠ、10mM Tris-Hcl、2mM  CaCl2、50%Glycerol (pH7.6, 25℃)\u003c\/p\u003e\n\u003cp\u003e10*ReactionBuffer: 100mM Tris-Hcl、25mM  MgCl2、5mM  CaCl2 (pH7.6, 25℃)\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eThis step is suitablefor linearization of 1 μg DNA (≥100 nt) and can be scaled up according toexperimental needs.\u003c\/p\u003e\n\u003cp\u003e1）Add the following components insequence\u003c\/p\u003e\n\u003ctable class=\"table table-bordered o_table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan style=\"font-size:10.5pt\"\u003eComponents\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eVolume\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003ePlasmid DNA\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e1μg DNA\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e10*Reaction Buffer\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e2μl\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eDnaseⅠ (2U\/μl)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e1μl\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eRNase-free ddH2O\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eUp to 50\u003cspan style=\"font-size:10.5pt\"\u003eμl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e2）Incubate at 37°C 1 h.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1.\tEDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation 2.\tPlease avoid repeated freeze-thaw cycles\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1KU","offer_id":41518782644299,"sku":"UA070036-1KU","price":80.0,"currency_code":"USD","in_stock":true},{"title":"5KU","offer_id":41518782677067,"sku":"UA070036-5KU","price":305.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b0fc9134-ed0d-4f24-9da1-c4142ebe1dc9.png?v=1787623339"},{"product_id":"bsa","title":"BsaⅠ Restriction Enzyme","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBacillus stearothermophilus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBsaI restriction endonuclease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e64.9kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e10 mM Tris-HCl、300 mM NaCl、1 mM DTT、0.1 mM EDTA、500 µg\/ml BSA、50% Glycerol （pH 7.4 @ 25°C）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eStore at -25 ~ -15℃for 2 years\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e[1]Zhu Z, Samuelson J C, Zhou J, et al.Engineering Strand-specific DNA NickingEnzymes from the Type IIS Restriction Endonucleases BsaI, BsmBI, andBsmAI[J].Journal of Molecular Biology, 2004,337(3):573-583. \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e[2] Lee J H, Won H J, Oh E S,etal. Golden GateCloning-Compatible DNA Replicon\/2A-Mediated Polycistronic Vectors forPlants[J]. Frontiers in Plant Science, 2020, 11.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eBsaIis a Type IIs restriction enzyme that can recognize non-palindromic sequencesand cut outside of the recognition sequence. It is commonly used for GoldenGate assembly and enzymatic cleavage of plasmids to prepare linear DNAfragments with poly (A\/T\/G\/C) endings and obtain specific sticky ends.\u003c\/p\u003e\n\u003cp\u003eRecognitionsite:\u003c\/p\u003e\n\u003cp\u003e5'-GGTCTC(N)1↓-3'\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e3'-CCAGAG(N)5↑-5'\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eStorageSolution: 20 U\/μl BsaⅠ、10 mM Tris-HCl、300 mM NaCl、1 mM DTT、0.1 mM EDTA、500 µg\/ml BSA、50% Glycerol（pH 7.4 @ 25°C）\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e10*Reaction Buffer：500 mM Potassium Acetate、200 mM Tris-acetate、100 mM Magnesium Acetate、1mg\/ml BSA（pH 7.4 @ 25°C）\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eThisstep is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled upaccording to experimental needs.\u003c\/p\u003e\n\u003cp\u003e1）Add the following components insequence\u003c\/p\u003e\n\u003ctable style=\"width: 384.986px;\" class=\"table table-bordered o_table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 195.097px;\"\u003e\u003cspan style=\"font-size:10.5pt\"\u003eComponents\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 189.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eVolume\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003ePlasmid DNA\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e1μg DNA\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e10*Reaction Buffer\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e5μl\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eBsaⅠ (20 U\/μl)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e1μl\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eRNase-free ddH2O\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 433.889px;\"\u003e\u003cp\u003eUp to 50\u003cspan style=\"font-size:10.5pt\"\u003eμl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e2）Incubate at 37°C 1h\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e3）DNA linearization iscomplete, and subsequent experiments can be performed.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1. star activity may result from a glycerol concentration of \u0026gt;5%; 2. Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1KU","offer_id":41518788837451,"sku":"UA070035-1KU","price":30.0,"currency_code":"USD","in_stock":true},{"title":"20KU","offer_id":41518788870219,"sku":"UA070035-20KU","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0eeb3efc-a532-4ec1-a60a-7c4d304f8da9.png?v=1789113663"},{"product_id":"idez-protease-tag-free","title":"IdeZ Protease (Tag Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus equi subsp. Zooepidemicus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIdeZ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e36kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTris-HC, pH7.5.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; \u003cbr\u003e\u003cbr\u003e1 week, 2 to 8 °C under sterile conditions; \u003cbr\u003e\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e[1] Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZProtease (Tag Free) in Glycan Profiling of Therapeutic Antibodies[J].Glycobiology, 2016(12):26.\u003c\/p\u003e\n\u003cp\u003e[2]Cheng-Wei H, JieP, Yu-Qing O ,et al. Characteristics of IgG degradation enzymeIdeZ in Streptococcus equi ssp. zooepidimicus[J].Chinese Journal of PreventiveVeterinary Medicine, 2018..\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eIdeZ Protease secreted by the Streptococcus equisubspecies zooepidemicus. This product is an engineered recombinant proteaseover expressed in Escherichia coli. It can specifically identify IgG, andperform enzyme digestion at specific sites in the hinge region of the antibody,and produce F(ab')2 fragments and Fc fragments, which can identify human and otheranimal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG,etc, It can be used for structural characterization analysis of antibodies andfusion protein drugs.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003ca\u003e\u003cspan style=\"font-size:10.5pt\"\u003e20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Add the desired amount of 5mg IgG indigestion buffer or other compatible buffer*.\u003c\/p\u003e\n\u003cp\u003e2. Add IdeZ Protease (Tag Free)-1 to thereaction system:\u003c\/p\u003e\n\u003cp\u003e• Add 1 unit of IdeZ Protease (Tag Free)-1 per1µg of IgG to be digested.\u003c\/p\u003e\n\u003cp\u003e• For example, add 5µl (200 units) ofreconstituted IdeZ to digest 200µg of IgG.\u003c\/p\u003e\n\u003cp\u003e3. Incubate sample at 37°C for 30–60minutes\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4000U","offer_id":41518792245323,"sku":"UA070033-4000U","price":385.0,"currency_code":"USD","in_stock":true},{"title":"40000U","offer_id":41518792278091,"sku":"UA070033-40000U","price":2135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9a541234-fd1e-41f6-bbfa-8854f4a446e5.png?v=1787630574"},{"product_id":"ultranuclease","title":"UltraNuclease","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerratia marcescens\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSMNE,Nuclease,UltraNuclease,Benzonase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003eAsp22-Asn266\u003c\/p\u003e\n\u003cp\u003eDTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e27.7kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e1.Nestle M, Roberts W K. An Extracellular Nuclease from Serratia marcescens I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME[J]. Journal of Biological Chemistry, 1969, 244.\u003c\/p\u003e\n\u003cp\u003e2.Kim W Y, Lee H S, Suh S J, et al. Purification and Cellular Localization of Extracellular Nuclease of Serratia marcescens Expressed in Escherichia coli[J]. Korean Journal of Microbiology, 1994, 32(2):147-154.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eMulti Nuclease all-around nuclease, also called broad-spectrum nucleic acid enzyme, is a kind of comes from Serratia Marcescens restriction endonuclease. It is capable of degradation of all forms of DNA and RNA (double-stranded, single-stranded, linear, circular or superhelical forms) under a very wide range of conditions (6Murea, 0.1M GuanidineHCl, 0.4%TritonX100, 0.1%SDS, 1mM EDTA, 1mM PMSF). The formation of 3-5 oligonucleotide residues containing 5 '-phosphate terminus is widely used to remove nucleic acids from biological products. The expression and purification of this product in Escherichia coli(E.coli) through genetic engineering can not only reduce the viscosity of cell supernatant and cell lysate in scientific research, but also improve the efficiency of protein purification and functional research. It can also be used in virus purification, vaccine production, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing the host residual nucleic acid to the peak (pg) level to improve the efficacy and safety of biological products. And can effectively prevent human peripheral blood monocyte (PBMC) clumping in cell therapy and vaccine research.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable width=\"690\" style=\"height: 100.417px;\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 33.4722px;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"491\"\u003e\u003cp\u003e\u003cspan\u003eAmount\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33.4722px;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003e\u003cspan\u003eUltraNuclease *\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"491\"\u003e\u003cp\u003e\u003cspan\u003e250U\/μL\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 33.4722px;\"\u003e\n\u003ctd width=\"198\"\u003e\u003cp\u003e\u003cspan\u003eBuffer Formulation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"491\"\u003e\u003cp\u003e\u003cspan\u003e10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e*\u003c\/span\u003e One unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1.Sample preparation:\u003c\/p\u003e\n\u003cp\u003eAdherent cells: Remove the medium, clean the cells with PBS, and remove the supernatant.\u003c\/p\u003e\n\u003cp\u003eSuspension cells: Cells were collected by centrifugation, cleaned with PBS, centrifuged at 6,000rpm for 10min, and precipitates were collected.\u003c\/p\u003e\n\u003cp\u003eEscherichia coli: The bacteria were collected by centrifugation, cleaned once with PBS, centrifuged at 8,000rpm for 5min, and precipitates were collected.\u003c\/p\u003e\n\u003cp\u003e2.Sample treatment:\u003c\/p\u003e\n\u003cp\u003eThe collected cell precipitates are cleaved according to the ratio of mass (g) to volume (mL) to 1: (10~20). Cells can also be cleaved mechanically or chemically on ice or at room temperature (1g cells are about 109).\u003c\/p\u003e\n\u003cp\u003e3.Enzyme addition:\u003c\/p\u003e\n\u003cp\u003ethe proportion of 1g cell precipitation digested by 250Units is required. You can also choose the addition plan according to the recommended dosage in the table above, increase the amount of enzyme within a certain range, and reduce the digestion time accordingly.\u003c\/p\u003e\n\u003cp\u003e4.Supernatant acquisition:\u003c\/p\u003e\n\u003cp\u003eThe supernatant of cell lysis solution was obtained by centrifugation at 12,000rpm for 30min, and then subsequent related experiments were conducted.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eConditional parameter\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eOptimum condition\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eApplicable condition\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eMg2+\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e1-2mM\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e1-10mM\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003ePH\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e8.0\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e6-10\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eTemperature\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e37℃\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-42℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eDTT\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-100mM\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e\u0026gt;0mM\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eβ-Me\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-100mM\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e＞0mM\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003eMonovalent cation\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-20mM\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-150mM\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003ephosphate anion\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-10mM\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"33%\"\u003e\u003cp\u003e0-100mM\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eOne unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25KU","offer_id":41518873215051,"sku":"UA070013-25KU","price":70.0,"currency_code":"USD","in_stock":true},{"title":"50KU","offer_id":41518873247819,"sku":"UA070013-50KU","price":115.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/792d05f7e54b406bbc04e348df3331ff.jpg?v=1787644975"},{"product_id":"luciferase","title":"Luciferase Ⅰ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePhotinus pyralis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eN.A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e62kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e25 mM Tris-acetate (pH7.8),1 mM EDTA, 1 mM DTT, 50% glycerol, 0.2 M ammonium sulfate\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eN.A\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81.\u003c\/p\u003e\n\u003cp\u003e 2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eLuciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. The Luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, wild-luciferase activity is inhibited by sodium chloride, Therefore, the amino acid mutation we performed improved the inhibition of sodium chloride and was eventually named Luciferase Ⅰ.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41518899757131,"sku":"UA070029-1mg","price":85.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41518899789899,"sku":"UA070029-5mg","price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/7929640a-b14d-4afa-a13c-8d52cbfd4640.png?v=1787648646"},{"product_id":"hrv-3c-protease","title":"HRV 3C Protease","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtease 3C、PreScission Protease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e21 kD (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v\/v) glycerol，（pH 8.0 @ 25℃）\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e[1] Knott J A , Orr D C , Montgomery D S ,et al.The expression and purification of human rhinovirus protease 3C.[J].European Journal of Biochemistry, 1989, 182(3):547-55.\u003c\/p\u003e\n\u003cp\u003e[2] Xu H , Wang Q , Zhang Z ,et al.A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease[J].Enzyme and Microbial Technology, 2019, 123:15-20.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eRecombinant human rhinovirus(HRV)3C protease is widely used in the purification and productions of proteins due to its good stability，high specificity，and enzymatic activity．3C protease is one of the non—structural proteins of HRV，and plays important roles in the life cycle of HRV．This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 1U\/μl 3C Protease50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v\/v) glycerol（pH 8.0 @ 25℃）10*Reaction Buffer: 500mM Tris-HCl，1.5M NaCl，10mM EDTA，10mM DTT，（pH 7.5@ 25℃）\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e1. Combine 100 μg of fusion protein, 10 μl of 10*Reaction Bufferr and H20 (if necessary) to make a 100 μl total reaction volume.2. Add 1 µl HRV 3C Protease3. Incubate reaction at 5°C for 16 hour.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 90% of the GST label from 100 µg of fusion protein at 5°C for16 hour.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100U","offer_id":41519844589643,"sku":"UA070044-100U","price":35.0,"currency_code":"USD","in_stock":true},{"title":"500U","offer_id":41519844622411,"sku":"UA070044-500U","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/83c0720c96a84a99a58fd7c494c865f9.png?v=1787670038"},{"product_id":"recombinant-sumo-protease-yeast","title":"Recombinant SUMO Protease, Yeast","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eYeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e27 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE and RP-HPLC\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM PB, pH7.4, 200mM NaCl, 0.1% NP-40, 0.5mM DTT, 50%(v\/v) Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· 6 months, -20 to -70 °C under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSaccharomyces cerevisiae-derived Small ubiquitin-like modifier (SUMO, Smt3) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a Saccharomyces cerevisiae-derived SUMO-specific protease(Ulp1) is then used to remove SUMO tag from these proteins in a ‘scarless’ manner. SUMO Protease cleaves in a highly specific manner, recognizing the tertiary structure of the SUMO tag, rather than an amino acid sequence, and hydrolyzes the peptide bond in the x-Gly-Gly-x sequence after the Gly-Gly bond at the C-terminus of the SUMO tag. The SUMO Protease cleavage proteins over wide ranges of temperature (4℃-30℃), ionic strengths(0-400 mM NaCl) and pH(7.0-9.0), and easily removed from the cleavage reaction by Immobilized Metal Affinity chromatography (IMAC).\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. SUMO Protease;\u003c\/p\u003e\n\u003cp\u003e2. 10X SUMO Protease Buffer + Salt: 500 mM PB, pH 7.4,2% Igepal (NP-40), 1.5 M NaCl, 10 mM DTT;\u003c\/p\u003e\n\u003cp\u003e3. 10X SUMO Protease Buffer – Salt: 500 mM PB, pH 7.4,2% Igepal (NP-40), 10 mM DTT;\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1.Add the following to a microcentrifuge tube:\u003c\/p\u003e\n\u003ctable style=\"width: 338.871px;\" class=\"table table-bordered o_table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 257.76px;\"\u003e\u003cp\u003eFusion Protein\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 80.0173px;\"\u003e\u003cp\u003e20μg\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e10X SUMO Protease Buffer +\/– Salt\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003e5μl\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003eSUMO \u003cspan style=\"font-size:10.5pt\"\u003eProtease \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003e10U\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eddH2O\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 718.125px;\"\u003e\u003cp\u003eTo 50μl\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e2. Mix and incubate at 30°C, Remove 5μl aliquots at1, 2, 4, and 6 hours.\u003c\/p\u003e\n\u003cp\u003e3. Analyze by SDS-PAGE. \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eKeep the concentration of Imidazole less then 150mM, or the activity ofthe SUMO Protease can be adversely affected.\u003c\/li\u003e\n\u003cli\u003eFor most fusion proteins, SUMO Protease functionsoptimally in a reaction mixture containing 150 mM NaCl; however, conditions maybe optimized by varying the NaCl concentration from 100 mM to 300 mM. Rememberto take into account the contribution of salt from the enzyme and from yoursubstrate. When setting up your cleavage reaction, use the appropriate 10X SUMOProtease Buffer +\/- Salt.\u003c\/li\u003e\n\u003cli\u003eResearchers need to optimize their specificreaction conditions. As an initial suggestion, 20 units of SUMO protease can beused per 40μg of target protein for 1 hour at 30 °C, or overnight at 2–8 °C.The cleavage efficiency can then be estimated by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of SUMO Protease cleaves ≥85% of 2 μg control substrate in 1 h at 30°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1kU","offer_id":41518967816267,"sku":"UA070004-1kU","price":140.0,"currency_code":"USD","in_stock":true},{"title":"5kU","offer_id":41518967849035,"sku":"UA070004-5kU","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/02717a82-6b9d-4aae-816b-7969fc156d95.png?v=1787659341"},{"product_id":"recombinant-tobacco-etch-virus-protease-rtev","title":"Recombinant Tobacco Etch Virus Protease (rTEV)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTobacco Etch Virus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003erTEV,TEV,RTEV\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e25U\/μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e28 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞98% by SDS-PAGE \u0026amp; RP-HPLC\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM EDTA, 5mM DTT, 50%(v\/v) Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· 6 months, -20 to -70 °C under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins. TEV protease has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln-Gly\/Ser [ENLYFQ(G\/S)] and cleavage occurs between the Gln and Gly\/Ser residues, The most commonly used sequence is ENLYFQG. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni\u003csup\u003e2+\u003c\/sup\u003e affinity resin. Recombinant Tobacco Etch Virus Protease (rTEV) has (NIa) protease catalytic domain which corresponds to a molecular weight of 28 kDa. It is unique with high specificity and is active at low temperature. rTEV Protease has a 6*His-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003col\u003e\n\u003cli\u003eTEV Protease;\u003c\/li\u003e\n\u003cli\u003e10X TEV Protease Buffer + Salt: 500mM Tris-HCl, pH8.0, 500mM NaCl, 5mMEDTA, 10mM DTT;\u003c\/li\u003e\n\u003cli\u003e10X TEV Protease Buffer – Salt: 500mM Tris-HCl, pH8.0, 5mM EDTA, 10mM DTT;\u003c\/li\u003e\n\u003c\/ol\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e1. Add the following to a microcentrifuge tube:\u003c\/p\u003e\n\u003ctable style=\"width: 391.858px;\" class=\"table table-bordered o_table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 269.76px;\"\u003e\u003cp\u003eFusion Protein\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 120.986px;\"\u003e\u003cp\u003e15\u003cspan style=\"font-size:10.5pt\"\u003eμg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e10X rTEV Protease Buffer +\/– Salt\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e5μl\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003erTEV Protease \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003e5U\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eddH2O\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 448.958px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eTo 50μl\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e2. Mix and incubate at 30°C, Remove 10 μL aliquotsat 1, 2, 4, and 6 hours.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3. Analyze by SDS-PAGE. \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eGuidelines for Cleavage\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor most fusion proteins, TEV Protease functionsoptimally in a reaction mixture without NaCl; however, conditions may beoptimized by varying the NaCl concentration from 0 mM to 500 mM. Remember totake into account the contribution of salt from the enzyme and from yoursubstrate. When setting up your cleavage reaction, use the appropriate 10X rTEVProtease Buffer +\/- Salt.\u003c\/li\u003e\n\u003cli\u003eResearchersneed to optimize their specific reaction conditions. As an initial suggestion, 10units of rTEV protease can be used per 30μg of target protein for 1 hour at 30°C, or overnight at 2–8 °C. The cleavage efficiency can then be estimated bySDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of TEV protease cleaves ≥85% of 3 μg of control substrate in 1 hour at pH 8.0 \u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1kU","offer_id":41518968701003,"sku":"UA070003-1kU","price":55.0,"currency_code":"USD","in_stock":true},{"title":"10kU","offer_id":41518968733771,"sku":"UA070003-10kU","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e87dd502-e076-423b-a2a8-8bdc2e08ad9e.png?v=1789376623"},{"product_id":"proteinase-k","title":"Proteinase K","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProteinase K\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePichiaPastoris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e29 kD\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 1 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19. \u003cbr\u003e[2] Xu C , Battig A , Schartel B ,et al. Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide)[J].Biomacromolecules, 2022, 23(11):4841-4850.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eProteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAdd the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg\/mL\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as amount of enzyme required to hydrolyze casein to produce 1µmoL L-tyrosine per minute under defined conditions.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10mg","offer_id":41519851372619,"sku":"UA070053-10mg","price":85.0,"currency_code":"USD","in_stock":true},{"title":"50mg","offer_id":41519851405387,"sku":"UA070053-50mg","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a7a8cfd3-4854-48bb-bd41-b043f7f94fee.png?v=1787688215"},{"product_id":"m-bovin-galt1y289l","title":"M-bovin-GalT1(Y289L)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eβ-1,4-galactosyltransferase Gal-T1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eTBS buffer filtered by 0.22μm filter membrane\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -80℃ for 6 months; Store at -20℃ for one month. Avoid repeated freeze-thaw.\u003cbr\u003eAfter opening the lid, store at -80℃ for 3 months.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003ePermissive bovine beta-1,4 galactosyltransferase mutant, utilizing UDP-GalNAz-Biotin, catalyzes the first enzymatic step of O-GlcNAc glycosylation modification by linking GalNAz to N-acetylglucosamine (GlcNAc). Mammalian expression system.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"200μg","offer_id":41519869034571,"sku":"UA070081-200μg","price":500.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41519869067339,"sku":"UA070081-1mg","price":1170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_24444e33-b7c6-41cd-8be5-8801d82d5e62.png?v=1789113659"},{"product_id":"recombinant-trypsin-ls","title":"Recombinant Trypsin LS","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e≥0.025 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;1.0 EU\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eDPBS solution (including 1.1mM EDTA)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cfont class=\"text-o-color-5\"\u003eThe product should be stored at 2-8 ° C for at least 12 months.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cfont class=\"text-o-color-5\"\u003eAvoid repeated freeze-thaw\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eThis product is a trypsin analogue derived from microorganisms, the recognition site is lysine or arginine, and the cutting site is the carboxyl end of arginine or arginine residues. It does not contain any animal-derived ingredients, has no animal-derived virus contamination (such as swine influenza virus, porcine parvovirus, etc.), lacks chymotrypsin activity naturally, and is a substitute for pig or bovine trypsin. This product has mild action on digestion cells and is stable and easy to store.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eInstructions for Use (for reference only)\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e1. Preheat the recombinant trypsin LS and complete medium at 37℃ before use.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e2. Discard the supernatant of the culture medium, wash the cells with an appropriate amount of DPBS, and discard the DPBS.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e3. Add an appropriate amount of Recombinant Porcine Trypsin LS (0.5 ml\/10 cm2) to the cells and incubate at 37°C until the cells detach.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e4. Add 5-10 ml of preheated complete culture medium, gently mix the cells, and transfer the cell suspension to a sterile 15 ml centrifuge tube. Centrifuge at 100g for 5-10 minutes.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e5. Discard the supernatant, and add an appropriate amount of complete culture medium for cultivation.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100ml","offer_id":41519869329483,"sku":"UA070087-100ml","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500ml","offer_id":41519869362251,"sku":"UA070087-500ml","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/28b21fb7-3295-4d12-a241-8ac022e8cebf.png?v=1787969017"},{"product_id":"luciferase-ua070030","title":"Luciferase Ⅱ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePhotinus pyralis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e61.7kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris (pH7.4), 1 mM EDTA, 1 mM DTT, 50% glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81.\u003c\/p\u003e\n\u003cp\u003e2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eLuciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. In our work, the luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, some biological applications are limited by the low stability of the luciferase, so we combined amino acid mutations to enhance the enzyme’s thermostability and was eventually named Luciferase Ⅱ.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41677959790667,"sku":"UA070030-1mg","price":85.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41677959823435,"sku":"UA070030-5mg","price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0542a456-925d-4491-bba8-def52521b96c.png?v=1787648640"},{"product_id":"rnase-h","title":"RNase H","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRibonuclease HI、RNase HI、Ribonuclease H、RNase H\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e19 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg\/ml BSA50% Glycerol（pH 7.4 @ 25°C）\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e [1] Chan S H , Whipple J M , Dai N ,et al.RNase H-based analysis of synthetic mRNA 5' cap incorporation[J].RNA (New York, N.Y.), 2022, 28(8):1144-1155.\u003c\/p\u003e\n\u003cp\u003e[2] Ilina T V , Brosenitsch T , Sluis-Cremer N ,et al.Retroviral RNase H: Structure, mechanism, and inhibition[J].  2021. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eEndonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA).\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 5U\/μl RNase H、50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg\/ml BSA 50% Glycerol（pH 7.4 @ 25°C）10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100mM DTT(pH 8.3 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSet-up a typical reaction as follows1）Add the following components in sequence2）Incubate at 37°C for 20 minutesThis reaction can be scaled up according to experimental needs.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1、Both metal ion chelating agent and sulfhydryl sealer can inhibit RNase H activity2、Heating at 65℃ can be inactivated for 10min\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme required to hydrolyze 1 nmol nucleotide from a 20 pmol fluorescent-labeled 50 bp RNA-DNA hybridization chain at 37℃ for 20 minutes in a 50 µl reaction system.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41677990330443,"sku":"UA070045-250U","price":70.0,"currency_code":"USD","in_stock":true},{"title":"1250U","offer_id":41677990363211,"sku":"UA070045-1250U","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b4bb8fcefbb14d47a9090883fb287038.png?v=1787673692"},{"product_id":"rnase","title":"RNase Ⅲ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRibonuclease 3,Ribonuclease III,RNase III\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e70 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol（pH 8 @ 25°C）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Nicholson AW. Ribonuclease III mechanisms of double-stranded RNA cleavage. Wiley Interdiscip Rev RNA. 2014 Jan-Feb;5(1):31-48. [2] Wu CX, Xu XJ, Zheng K, Liu F, Yang XD, Chen CF, Chen HC, Liu ZF. Characterization of ribonuclease III from Brucella. Gene. 2016 Apr 1;579(2):183-92. [3] Sun, Weimei et al. Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis. Nucleic Acids Research 33 (2005): 807 - 815.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe processing of double-stranded(ds) RNA by RNase III family members is an essential step in the maturation and decay of coding and noncoding RNAs, including miRNAs and siRNAs. RNase III family members share a unique fold (RNase III domain) that can dimerize to form a structure that binds dsRNA and cleaves phosphodiesters on each strand, providing the characteristic 2 nt, 3'-overhang product ends. RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 2U\/μl RNase Ⅲ、500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol（pH 8 @ 25°C）10*Reaction Buffer: 500 mM Tris-HCl、1 mM DTT 50 mM NaCl (pH 7.5 @ 25°C)10*EDTA: 500 mM10*MnCl2: 200 mM\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSet-up a typical reaction as follows1）Add the following components in sequence2）Incubate at 37°C for 20 minutesThis reaction can be scaled up according to experimental needs.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme required to digest 1 μg of dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 50 μl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"200U","offer_id":41677990395979,"sku":"UA070046-200U","price":65.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41677990428747,"sku":"UA070046-1000U","price":265.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/23399359ab4b4fdf8f30692ae84a030c.png?v=1787673695"},{"product_id":"t4-dna-ligase-ua070047","title":"T4 DNA Ligase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA Ligase、Polydeoxyribonucleotide synthase [ATP]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e57 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; RP-HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol（pH 7.4 @ 25°C）\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 1 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Williamson A, Pedersen H. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida. Protein Expr Purif. 2014 May; 97:29-36. \u003cbr\u003e[2] Liu X, Huang A, Luo D, Liu H, Han H, Xu Y, Liang P. Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli. Protein Expr Purif. 2015 May;109:79-84. Epub 2015 Feb 17. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eT4 DNA ligase is a type of DNA ligase. DNA ligase catalyzes the formation of phosphodiester bonds at single-stranded DNA breaks in double-stranded DNA in vivo. DNA ligase has important biological functions in organisms. In DNA repair and recombination, DNA ligase plays a role in connecting gaps. In the process of DNA replication, the synthesis of the lagging strand is discontinuous, and DNA ligase connects the discontinuous DNA strand into a continuous DNA strand. It does not contain DNA endonuclease, exonuclease and phosphatase, and does not contain RNA enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 400U\/μl T4 DNA Ligase、10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol (pH 7.4 @ 25°C)10*Reaction Buffer: 500 mM Tris-HCl、100 mM MgCl2、10 mM ATP、100 mM DTT (pH 7.5 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1、Set up the following reaction in a microcentrifuge tube on ice. Add the following components in sequence. Note that the table shows a ligation using\u003cspan style=\"font-size:10.5000pt\"\u003e1. a molar ratio of 1:5 vector to insert for the indicated DNA sizes.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume 20μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10* T4 DNA Ligase Buffer \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVector DNA (such as \u003cfont\u003ep\u003c\/font\u003eET-28a 5369\u003cfont\u003ebp\u003c\/font\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e50ng \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eInsert DNA (530bp)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e25ng \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNuclease-free water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eUp to 20 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eT4 DNA Ligase \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e2. Gently mix the reaction through the up and down pipette, and inhale the liquid briefly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e3. For sticky ends, incubate at 16°C overnight or at room temperature for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e4. For blunt ends or single base overhangs, incubate overnight at 16°C or room temperature for 2 hours (alternatively, high concentrations of T4DNA ligase can be used for 10 minutes of ligations).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cspan style=\"color:rgb(0,0,0);font-size:10.5000pt\"\u003e5. Chill on ice and transform 1-5 μl of the reaction into competent cells.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. ATP is an essential cofactor in the reaction. This is in contrast to E. coli DNA Ligase, which requires NAD as a cofactor. \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. If T4 DNA Ligase is to be diluted, it is recommended that it be diluted with 50% glycerol in storage buffer and stored at -20°C. 3. Room temperature ligation\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is the amount of enzyme required to ligate 50% of the HindIII-digested λ DNA fragments [DNA 5´-end concentration of 0.12 µM (300 μg\/ml)] within 30 minutes at 16°C in a 20 µl reaction system and in 1X T4 DNA Ligase Reaction Buffer.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"20KU","offer_id":41677998882891,"sku":"UA070047-20KU","price":35.0,"currency_code":"USD","in_stock":true},{"title":"100KU","offer_id":41677998915659,"sku":"UA070047-100KU","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/257d429b154a4de0aa317f037f9aad55.png?v=1787688111"},{"product_id":"proteinase-ktag-free","title":"Proteinase K(Tag free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProteinase K\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePichiaPastoris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e72 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 1 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19. \u003cbr\u003e[2] Xu C , Battig A , Schartel B ,et al.Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide)[J].Biomacromolecules, 2022, 23(11):4841-4850.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eProteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAdd the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg\/mL\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as amount of enzyme required to hydrolyze casein to produce 1µmoL L-tyrosine per minute under defined conditions.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10mg","offer_id":41677999243339,"sku":"UA070054-10mg","price":85.0,"currency_code":"USD","in_stock":true},{"title":"50mg","offer_id":41677999276107,"sku":"UA070054-50mg","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4de83be0f2c40ef83380d57951afda8.png?v=1787688217"},{"product_id":"dna-polymerase-i-large-klenow-fragment","title":"DNA Polymerase I Large (Klenow) Fragment","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eKlenow Fragment、DNA Polymerase I Large (Klenow) Fragment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e70kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Zhao, Guojie , et al. \"Realizing directional cloning using sticky ends produced by 3′-5′ exonuclease of Klenow fragment.\" Journal of Biosciences 38.5(2013):857-866.\u003cbr\u003e[2] Olsen, Tivoli J. , et al. \"Electronic Measurements of Single-Molecule Processing by DNA Polymerase I (Klenow Fragment).\" Journal of the American Chemical Society 135.21(2013):7855-7860.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe Klenow Fragment, is a large fragment of E.coli. DNA polymerase I. It retains the 3'→5' exonuclease activity of DNA polymerase I, but lacks the 5'→3' exonuclease activity of the intact DNA polymerase I. The 3'→5' exonuclease activity of Klenow Fragment ensures accurate proofreading when synthesizing DNA.  It is used to fill in the 5'overhang ends of double-stranded DNA; and double-stranded DNA 3'overhang flattening (also called trimming). It can also be used for the synthesis of the second strand of cDNA or the synthesis of the second strand of site-specific mutation reaction.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 5 U\/ul Klenow Fragment、25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C 10*Reaction Buffer: 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT(pH 7.9 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e1. DNA should be dissolved in 1*Reaction Buffer or T4 DNA Ligase Reaction buffer and supplemented with 33 μM each dNTP.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e2. Add 1 unit of DNA Polymerase I Large (Klenow) Fragment per microgram DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e3. Incubate for 15 minutes at 25°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e4. Stop reaction by adding EDTA to a final concentration of 10 mM and heating for 20 minutes at 75°C.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. Due to the 3´→5´ exonuclease activity of the enzyme, increasing the reaction temperature, adding too much enzyme, not adding dNTP or too long reaction time will lead to the formation of the dented end. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2. Please avoid repeated freeze-thaw cycles\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble substances at 37 ° C for 30 minutes.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"200U","offer_id":41678000422987,"sku":"UA070056-200U","price":95.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678000455755,"sku":"UA070056-1000U","price":335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/99c07c1d16af4460a02b9fcabda69c84.png?v=1787695209"},{"product_id":"ultra-nuclease-tag-free","title":"Ultra Nuclease (Tag free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSerratia marcescens\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNuclease, Ultra Nuclease, Benzonase、Endonuclease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e27kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Nestle M, Roberts W K. An Extracellular Nuclease from Serratia marcescens I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME[J]. Journal of Biological Chemistry, 1969, 244.\u003cbr\u003e2. Kim W Y, Lee H S, Suh S J, et al. Purification and Cellular Localization of Extracellular Nuclease of Serratia marcescens Expressed in Escherichia coli[J]. Korean Journal of Microbiology, 1994, 32(2):147-154.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eMulti Nuclease also called broad-spectrum nucleic acid enzyme, is a kind of comes from Serratia Marcescens restriction endonuclease. It is capable of degradation of all forms of DNA and RNA (double-stranded, single-stranded, linear, circular or super helical forms) under a very wide range of conditions (6Murea, 0.1M Guanidine, 0.4%TritonX100, 0.1%SDS, 1mM EDTA, 1mM PMSF). The expression and purification of this product in Escherichia coli(E.coli) through genetic engineering can not only reduce the viscosity of cell supernatant and cell lysate in scientific research, but also improve the efficiency of protein purification and functional research. It can also be used in virus purification, vaccine production, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing the host residual nucleic acid to the peak (pg) level to improve the efficacy and safety of biological products. And can effectively prevent human peripheral blood monocyte (PBMC) clumping in cell therapy and vaccine research.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: ≥250U\/μl Ultra Nuclease (Tag free)、10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol \u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. Sample preparation:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eAdherent cells: Remove the medium, clean the cells with PBS, and remove the supernatant.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSuspension cells: Cells were collected by centrifugation, cleaned with PBS, centrifuged at 6,000rpm for 10min, and precipitates were collected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eEscherichia coli: The bacteria were collected by centrifugation, cleaned once with PBS, centrifuged at 8,000rpm for 5min, and precipitates were collected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2. Sample treatment: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eThe collected cell precipitates are cleaved according to the ratio of mass (g) to volume (mL) to 1: (10~20). Cells can also be cleaved mechanically or chemically on ice or at room temperature (1g cells are about 109).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3. Enzyme addition: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003ethe proportion of 1g cell precipitation digested by 250Units is required. You can also choose the addition plan according to the recommended dosage in the table above, increase the amount of enzyme within a certain range, and reduce the digestion time accordingly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4. Supernatant acquisition: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eThe supernatant of cell lysis solution was obtained by centrifugation at 12,000rpm for 30min, and then subsequent related experiments were conducted.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eRecommended reaction condition:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eConditional parameter\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eOptimum condition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eApplicable condition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eMg2+\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1-2mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1-\u003cfont\u003e10\u003c\/font\u003emM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003ePH\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e8.0\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e6-10\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTemperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e37℃\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-42℃\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDTT\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0-100mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u0026gt;0mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eβ-\u003c\/font\u003eM\u003cfont\u003ee\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-100mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e＞\u003cfont\u003e0mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eMonovalent cation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-20mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-150mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003ephosphate anion\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-10mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0-100mM\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25KU","offer_id":41678000488523,"sku":"UA070057-25KU","price":70.0,"currency_code":"USD","in_stock":true},{"title":"50KU","offer_id":41678000521291,"sku":"UA070057-50KU","price":115.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/df684e5bf1034b708d95c4c64d51feb5.jpg?v=1787695211"},{"product_id":"endoproteinase-glu-c","title":"Endoproteinase Glu-C, Mass Spectrometry Grade","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStaphylococcus aureus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGlutamyl endopeptidase、V8 protease、Endoproteinase Glu-C、V8 proteinase、V8、Glu-C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e25kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eTo get the most use out the enzyme, resuspend the enzyme in 500 μl H2O and aliquot 50 ul each in 10 tubes.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Yabuta M , Ochi N , Ohsuye K .Hyperproduction of a recombinant fusion protein of Staphylococcus aureus V8 protease in Escherichia coli and its processing by OmpT protease to release an active V8 protease derivative.[J].Appl Microbiol Biotechnol, 1995, 44(1-2):118-125.\u003cbr\u003e[2] Prasad L , Leduc Y , Hayakawa K ,et al. The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus[J].Acta Crystallographica Section D Biological Crystallography, 2004.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStaphylococcus aureus V8 protease (Endoproteinase Glu-C) belongs to the serine protease family and can specifically hydrolyze carboxy-terminal peptide bonds of glutamic acid (Glu) or aspartate (Asp) residues. Its specificity is directly affected by the composition of the buffer, recognizing and cutting Glu carboxy-terminal peptide bonds in NH4HCO3 of pH7.8 and CH3COONH4 of pH4.0, recognizing and cutting Glu or Asp carboxy-terminal peptide bonds in phosphate buffers of pH7.8，but the hydrolysis rate of Glu was higher than that of Asp. Glu-C has been reported to be active in 0.2% SDS (sodium lauryl sulfate) and 4.0M urea.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSupplied in dry format from Tris-HCl and sodium chloride buffer\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eFor the digestion of peptides or proteins, the ratio of enzyme to  substrate between is 1:20 and 1:100 (w\/w) for recommended. Dissolve the peptide or protein and was enzymolysis in 100 mM NH4HCO3, pH 7.8 or 100 mM Tris-HCl, pH 7.8, the recommended incubation time is 2-18 hours at 37°C, depending on the temperature and the ratio of enzyme to substrate.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eRepeated freeze\/thawing is not recommended.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eUsing 2-Ph-leu-Glu-4-Na as substrate, the amount of enzyme required to produce 1μmol 4-Nitroaniline in 1 minute at pH7.8, 25℃ was 1unit.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"50μg","offer_id":41678002618443,"sku":"UA070059-50μg","price":130.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/73612da055224dcd858617b9d49fb1c8.png?v=1787902060"},{"product_id":"recombinant-carboxypeptidase-bbovine","title":"Recombinant Carboxypeptidase B，Bovine","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBovine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCarboxypeptidase B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePichiaPastoris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e33 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Tris-HCl 、100mM NaCl\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eCarboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg \/ml.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e·24 month, 2 to 8 °C under sterile conditions \u003cbr\u003e. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.\u003cbr\u003e[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eCarboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe recommended amount of Carboxypeptidase B is 1:50~1:1000 (w\/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eThe amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41678002847819,"sku":"UA070058-1mg","price":125.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":43082311729227,"sku":"UA070058-5mg","price":230.0,"currency_code":"USD","in_stock":true},{"title":"10mg","offer_id":41678002880587,"sku":"UA070058-10mg","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f0d3813b6bba4df19780597c3347ce2d.png?v=1787904083"},{"product_id":"phi29-dna-polymerase","title":"phi29 DNA Polymerase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ephi29 DNA Polymerase、DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e68kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e≥95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e100 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.5% Tween® 20、50% Glycerol、0.5% NP40、pH 7.4 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Tadas P, Gediminas A , Rasa S ,et al. In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self-replication technique[J].Protein Engineering Design \u0026amp; Selection, 2016(12):617-628.\u003cbr\u003e2. Margarita S, Isabel H, Redrejo-Rodríguez Modesto,et al. DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication[J].Frontiers in Molecular Bioences, 2016, 3:37. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003ephi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 (Φ29) (1). On the one hand, the enzyme has excellent strand replacement and sustained synthesis capabilities, enabling the unstranding and replication of complex DNA structures and isothermal DNA polymerization reactions in vitro that do not depend on thermal cycling. On the other hand, the enzyme possesses 3´→5´ nucleic acid exonuclease proofreading activity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e10U\/μl phi29 DNA Polymerase、100 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.5% Tween® 20、50% Glycerol、0.5% NP40、pH 7.4 @ 25°C\u003cbr\u003eReaction buffer: 50 mM Tris-HCl、10 mM MgCl2、10 mM (NH4)2SO4、4 mM DTT、(pH 7.5 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e1. Prepare the reaction system on the ice bath with reference to the table below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 529.662px;\" class=\"table table-bordered\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 255.921px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 113.007px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 160.009px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e10X Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e2 \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003edNTP (2.5 mM each)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e25 uM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eRandom Hexamer Primers (100μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e5\u003c\/font\u003e mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eTemplate DNA (≥1 ng)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003ex\u003c\/font\u003e \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eNuclease-free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e(15-\u003cfont\u003ex\u003c\/font\u003e) \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003eTotal Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.222px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e9 \u003cfont\u003eul\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 382.234px;\"\u003e\u003cp\u003e\u003cspan style=\"color:rgb(33,37,41);font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 12px;\"\u003e*phi29 DNA Polymerase has a strong 3'→5' exonuclease activity. If the amplification effect is not good, it is recommended to reduce the amount of enzyme in the reaction system to 0.5μl or 0.25μl.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e2. Predegeneration of template DNA: The reaction system was incubated in a PCR apparatus at 95 ℃ for 5min, and quickly placed in an ice bath for 2min or longer.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e3. Isothermal amplification reaction: 1μl phi29 DNA Polymerase was added into the cooled reaction system and incubated at 30℃ for 2-16h. Incubation is usually sufficient for 2h, and if a larger amount of amplified product is desired, the incubation time can be extended to 16h. It is recommended to use a constant temperature water bath for the reaction. If using a hot cap PCR apparatus, adjust the hot cap temperature to 40 ° C to avoid enzyme inactivation.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e4. Termination reaction: incubation at 65℃ for 10 min.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px;\"\u003e5. Detection of amplification products: The amplification products were subjected to agarose gel electrophoresis to detect the amplification effect.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. The active reducing agent in the reaction buffer is critical for this enzyme. Although the reaction buffer supplied with the enzyme contains DTT, in order to ensure maximum activity, 4 mM DTT should be added when using buffers that have been stored for long periods of time or buffers that have been repeatedly freeze-thawed.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2.Reaction temperatures above 65°C are not recommended.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3.The enzyme does not have 5´→3´ nucleic acid exonuclease activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit refers to the amount of enzyme required to catalyze the incorporation of 0.5 pmol of dNTP into an acid-insoluble material in 10 minutes at 30 °C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41678029389899,"sku":"UA070068-250U","price":95.0,"currency_code":"USD","in_stock":true},{"title":"1250U","offer_id":41678029422667,"sku":"UA070068-1250U","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c2f47c40def34e07a1eb5e9850060966.png?v=1787922130"},{"product_id":"t4-dna-polymerase","title":"T4 DNA Polymerase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA-directed DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e105kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e100 mM K3PO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Capson TL, Peliska JA, Kaboord BF, Frey MW, Lively C, Dahlberg M, Benkovic SJ. Kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage T4. Biochemistry. 1992 Nov 17;31(45):10984-94. \u003cbr\u003e2. Tanguy Le Gac N, Delagoutte E, Germain M, Villani G. Inactivation of the 3'-5' exonuclease of the replicative T4 DNA polymerase allows translesion DNA synthesis at an abasic site. J Mol Biol. 2004 Mar 5;336(5):1023-34. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eT4 DNA polymerase catalyzes the synthesis of DNA in the 5´→3´ direction and requires the presence of template and primer. This enzyme possesses 3´→5´ exonuclease activity, which is much higher than that found in DNA polymerase I (E. coli). Unlike E. coli DNA polymerase I, T4 DNA polymerase lacks 5’ →3’ exonuclease activity. T4 DNA polymerase can be used to Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e3 U\/μl T4 DNA Polymerase, 100 mM K3PO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C\u003cbr\u003e10* Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 1000 µg\/ml Recombinant Albumin, (pH 7.9 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003e\u003cbr\u003eIncubate the following reaction at 12°C for 15 minutes\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 512.662px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 296.921px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eComponent\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 215.012px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10X Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edNTP Mix (10 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e100 mM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eT4 DNA Polymerase (3,000 U\/ml)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1000 U\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDNA \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 ug\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNuclease-free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eto 10 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 573.333px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTotal Reaction Volume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 573.345px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003eStop reaction by adding EDTA to a final concentration of 10 mM and heating to 75°C for 20 minutes\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eElevated temperatures, excessive amounts of enzyme, failure to supplement with dNTPs or long reaction times will result in recessed ends due to the 3´ → 5´ exonuclease activity of the enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"150U","offer_id":41678031781963,"sku":"UA070070-150U","price":130.0,"currency_code":"USD","in_stock":true},{"title":"750U","offer_id":41678031814731,"sku":"UA070070-750U","price":490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/723c1777a45a4a7dbbb5ed5f9d495b11.png?v=1787922242"},{"product_id":"m-mlv-h-reverse-transcriptase","title":"M-MLV (H-) Reverse Transcriptase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eM-MLV[H-] RT,M-MLV[H-] Reverse Transcriptase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e76 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA、0.01% Nonidet® P-40、50% Glycerol pH 7.5 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Blain, S. W. , and  S. P. Goff . \"Differential Effects of Moloney Murine Leukemia Virus Reverse Transcriptase Mutations on RNase H Activity in Mg and Mn.\" Journal of Biological Chemistry 271.3(1996):1448-54.\u003cbr\u003e[2] Narukawa, Yutaro , et al. \"Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region.\" Protein Engineering, Design and Selection (2021).\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eM-MLV (H-) Reverse Transcriptase is an RNA-dependent DNA polymerase with reduced RNase H activity. This enzyme can use RNA (when synthesizing cDNA) or single-stranded DNA as a template and initiate the synthesis of a complementary DNA strand from a primer. M-MLV(H-) reverse transcriptase has no 3´ → 5´ exonuclease activity. It can be used to synthesize first strand cDNA more efficient than the wild type M-MuLV。\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution : 100 U\/ul M-MLV(H-) Reverse Transcriptase、20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA、0.01% Nonidet® P-40、50% Glycerol pH 7.5 @ 25°C\u003cbr\u003e5*Reaction Buffer: 250 mM Tris-HCl、375 mM KCl、15 mM MgCl2、50 mM DTT (pH 8.3 at 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e1.\tGenomic DNA was removed from the extracted cell total RNA by DNase I.\u003cbr\u003e2.\tAfter incubating at 37 °C for 30 minutes, add 1µl 0.5 M EDTA (to the final concentration of 5mm) and inactivate at 75°C for 10 minutes.\u003cbr\u003e3.\tMix RNA sample, Random Primer and 1 ul M-MLV(H-) Reverse Transcriptase in a sterile RNase-free microfuge tube.\u003cbr\u003e4.\tIncubate the 20 μl cDNA synthesis reaction was incubated at 25℃ for 5 minutes and then at 42℃ for 1 hour.\u003cbr\u003e5.\tInactivate the enzyme at 65°C for 20 minutes. The cDNA product can be directly fed into the qPCR reaction or stored at -20°C.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10000U","offer_id":41678033322059,"sku":"UA070066-10000U","price":40.0,"currency_code":"USD","in_stock":true},{"title":"50000U","offer_id":41678033354827,"sku":"UA070066-50000U","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b8920fd955f447dbbd1d00d03f2d9e3a.png?v=1787925665"},{"product_id":"t4-rna-ligase-1","title":"T4 RNA Ligase 1","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eT4 RNA Ligase,T4 Rnl1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e69kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol pH 7.4 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] England, Thomas E , and  O. C. Uhlenbeck . \"3|[prime]|-Terminal labelling of RNA with T4 RNA ligase.\" Nature 275.5680(1978):560-1.\u003cbr\u003e[2] Tessier, Daniel C. ,  R. Brousseau , and  T. Vernet . \"Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.\" Analytical Biochemistry 158.1(1986):171-178.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eT4 RNA Ligase 1 is an ATP-dependent enzyme that catalyzes the formation of phosphodiester bonds between the 5'-P and 3'-OH ends of single-stranded RNA, single-stranded DNA, or single nucleotides, either intermolecular or intramolecular. It is suitable for the connection of single-stranded RNA molecules and the 5' end junction constructed by miRNA libraries in NGS. Labeled with RNA 3 'terminal; Cyclization of oligonucleotides; tRNA modification; In 5'-RACE (Rapid Amplification of cDNA Ends) assay, it is used for oligonucleotides to bind to single-stranded cDNA; and the introduction of unnatural amino acids into proteins.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 688.604px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 234.863px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eName\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 166.002px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 128.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003equantity\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 159.009px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eT4 RNA Ligase 1 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e-20 ℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e100 ul or 500 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 U\/µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10*Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e-20 ℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eAdenosine 5'-Triphosphate (ATP) \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e-20 ℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003ePEG8000 (RNase free)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e-20 ℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e50%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Set up a 20 μl reaction as follows:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 638.604px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.863px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 95.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 157.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 * Reaction Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2 ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSingle-stranded RNA with 5´P and 3´OH ends (200 ng-1 µg)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10-50 ng\u003cfont\u003e\/\u003c\/font\u003eul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e50% PEG8000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e4\u003c\/font\u003e ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1mM ATP\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e50 µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e40 μ\/ul Rnase inhibitor    \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0\u003c\/font\u003e.5ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 U\/µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 U\/µl T4 RNA Ligase 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003eul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.5 U\/µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDEPC-treated Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eUp to 20ul\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e2. Incubate at 37°C for 1-2 hours.\u003cbr\u003eFor longer oligos, overnight incubation at 16°C for 16h may improve yield.\u003cbr\u003e3. Incubate at 65°C for 15min to terminate the reaction.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1000U","offer_id":41678270398539,"sku":"UA070071-1000U","price":100.0,"currency_code":"USD","in_stock":true},{"title":"5000U","offer_id":41678270431307,"sku":"UA070071-5000U","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5ca98e270c4b429baf0ebc96d2a33618.png?v=1787929293"},{"product_id":"pfu-dna-polymerase-ua070072","title":"Pfu DNA Polymerase Ⅱ","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA polymerase、 DNA polymerase B、Pfu polymerase、Pol I\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e90 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Lin, T. C. , et al. \"Cloning and expression of T4 DNA polymerase.\" Proceedings of the National Academy of Sciences 84.20(1987):7000-7004.\u003cbr\u003e[2] Yan, Wang , et al. \"A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro.\" Nucleic Acids Research 3(2004):1197.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003ePfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution : 5 U\/ul Pfu DNA Polymerase Ⅱ, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C \u003cbr\u003e10* Reaction Buffer: 200 mM Tris-HCl (pH 8.8) , 20 mM MgSO4, 100 mM KCl, 100 mM (NH4) 2SO4, 1% Triton X-100, 1 mg\/ml nuclease-free BSA\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Set up a 50 μl PCR reaction system as follows:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 659.595px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 246.863px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 178px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 233.991px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10X Pfu Buffer (with Mg2+)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edNTP mix, 10mM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003eµl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e200µM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eupstream primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5–50pmo\u003cfont\u003el\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.1–1.0µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edownstream primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5–50pmo\u003cfont\u003el\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.1–1.0µM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDNA template   \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003evariable\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u0026lt;0.5µg\/50µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003ePfu DNA Polymerase (5U\/µl)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003evariable\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.25U\/50µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.014px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDEPC-treated Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eUp to 50µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.025px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e 2. Recommended thermal cycling conditions for Pfu DNA Polymerase-mediated PCR amplification as follows:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 697.604px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 203.863px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eStep\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 155.009px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTemperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 157.009px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTime\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 181.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNumber of Cycles\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eInitial Denaturation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e95°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1–2 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDenaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eAnnealing*\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eExtension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e95°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e42–65°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e72–74°C\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.5–1 minute\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e30 seconds\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2–4 minutes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e25–35 cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFinal Extension\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e72–74°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSoak\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eIndefinite\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 297.766px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e*The annealing temperature for a specific amplification reaction will depend upon the sequences of the two primers.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eNote: It is critical to withhold Pfu DNA Polymerase until after the addition of dNTPs; otherwise, the proofreading activity of the polymerase may degrade the primers,resulting in nonspecific amplification and reduced product yield. Assemble on ice.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41678270955595,"sku":"UA070072-250U","price":140.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678270988363,"sku":"UA070072-1000U","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0c50fb9ae1c346019c2742450c04331c.png?v=1787929405"},{"product_id":"recombinant-trypsin-digestion-solution","title":"Recombinant trypsin digestion solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePIG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTrypsin(pig); porcine pancreas; Proenzyme; Parenzymol; Pseudotrypsin ;Tryptar; Trypure; \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.15mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e24 kD\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.9% sodium chloride, pH 7.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 month, 2 to 8 °C under sterile conditions \u003cbr\u003eStore at -20℃ and avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Charles M , Rovery M , Guidoni A ,et al.Trypsinogen and trypsin of pig[J].Biochimica et Biophysica Acta, 1963, 69:115-129.\u003cbr\u003e[2] Keryn,Dallas,Johnson,et al.A functional comparison of ovine and porcine trypsins[J].Comparative Biochemistry \u0026amp; Physiology Part B Biochemistry \u0026amp; Molecular Biology, 2002.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eTrypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. The stringent specificity of trypsin is essential for protein identification, and it has become the gold standard for protein digestion to peptides for shotgun proteomics. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl- L-arginine methyl ester (TAME), tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide (BAPNA), L-lysyl-p-nitroanilide, and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone), PMSF (phenylmethanesulfonyl fluoride), benzamidine, soybean trypsin inhibitor, and ovomucoid.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e1x, 0.15 g Recombinant porcine trypsin and 0.02 g EDTA per liter of 0.9% sodium chloride\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eTrypsin Solution may be used to remove adherent cells from a culture surface.\u003cbr\u003e1. Remove medium from culture vessel by aspiration and wash the monolayer with PBS to remove all traces of serum. Remove salt solution by aspiration.\u003cbr\u003e2. Dispense enough Trypsin Solution into culture vessel(s) to completely cover the monolayer of cells and place in RT or 37℃ incubator for 2-3 minutes.\u003cbr\u003e3. Remove the Trypsin Solution by aspiration and return closed culture vessel(s) to incubator. The coated cells are allowed to incubate until cells detach from the surface. Progress can be checked by examination with an inverted microscope.\u003cbr\u003e4.When trypsinization process is complete the cells will be in suspension and appear rounded.\u003cbr\u003e5. It is advisable to add serum or medium containing serum to the cell suspension as soon as possible to inhibit further tryptic activity which may damage cells.\u003cbr\u003e6. Cells can be resuspended by gently pipetting the cell suspension to break up the clumps. Further dilution can be made, if required, for cell counts and\/or subculturing.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eThe time required to remove cells from the culture surface is dependent on cell type, population density, serum concentration in the growth medium, potency of trypsin, and time since last subculture. Trypsin can cause cellular damage, thus time of exposure should be kept to a minimum.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eIn a 3.2ml reaction system using BAEE as substrate, the UV absorption at 253nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100mL","offer_id":41678273478731,"sku":"UA070069-100mL","price":70.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":41678273511499,"sku":"UA070069-500mL","price":230.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5530d188a99943719dae1fc144b48e71.png?v=1787950832"},{"product_id":"kex2","title":"Kex2","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eKexin,Protease KEX2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePichia pastoris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e75 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20mM NaAC-HAC，2mM Ca2+，ph5.2 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Kasai N .Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease[J].Journal of Biological Chemistry, 1992, 267(1):45-63.\u003cbr\u003e2. Matsuo T H . Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces, cerevisiae[J].Biochemical and Biophysical Research Communications, 1989.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eKex2 protease, a precursor processing protease derived from yeast, is a calcium-dependent serine protease that specifically recognizes and cleaves the carboxy-terminal peptide bond of the bi-alkaline amino acids Arg-Arg, Lys-Arg, and Pro-Arg. Unlike trypsin, Kex2 does not recognize and cleave the carboxy-terminal peptide bond of a single basic amino acid, arginine or lysine. In yeast, Kex2 protease is responsible for the processing of killer toxin and α-factor precursors. Kex2 protease activity is not inhibited by conventional serine protease inhibitors such as peptidyl peptidase, PMSF and TPCK. The recombinant Kex2 protease is produced by expression of Saccharomyces cerevisiae and has the same enzyme specificity as the natural Saccharomyces cerevisiae Kex2 enzyme. The optimal pH for action is pH 9.0, and the pH for stable storage is pH 5.0-6.0.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eKex2 lyophilized\u003cbr\u003eDissolved buffer: 20mmol\/L NaAc-HAc, 2mmol\/L Ca2+, pH 5.0~5.5\u003cbr\u003eReaction buffer:50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eKex2 should be reconstituted by the addition of 20–100 μl of Dissolved buffer. Typical reaction conditions are as follows:\u003cbr\u003eCombine 500 ug of sample with reaction buffer *（Recommended 50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C))）\u003cbr\u003eAdd 5μL(5μg)of Kex2，Incubate at 25°C for 3 hours\u003cbr\u003eOptimal incubation times and enzyme concentrations must be determined empirically for a particular substrate Recommended ratio: protease: fusion protein amount ratio 1:20~1:1000\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1.Reaction buffer: pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+;\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2.If the lyophilized powder cannot be used immediately after dissolution, it is recommended to dissolve the lyophilized powder with 20mM (pH5.2) NaAc-HAc, 2mM Ca2+ buffer;\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3.The final concentration of the enzyme after dissolution will be stored at -20℃ for storage after dispensing as needed;\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e4.The optimal reaction pH for Kex2 is pH 9.0, and the optimal pH for storage is 5.0-6.0.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit refers to the amount of enzyme required to catalyze the conversion of 1 µmol of BOC-Gln-Arg-Arg-Arg-PNA to the product per minute at 25°C, pH 8.0.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100µg","offer_id":41678289403979,"sku":"UA070082-100µg","price":125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1f2cadb49bf244fbb5c4a4fce082c8ed.png?v=1787968982"},{"product_id":"pfu-dna-polymerase-ua070083","title":"Pfu DNA Polymerase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePyrococcus furiosus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDNA polymerase、 DNA polymerase B、Pfu polymerase、Pol I\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e90 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Picard Véronique, et al. \"A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase.\" Nucleic Acids Research 22.13(1994):2587-91.\u003cbr\u003e[2] Yan, Wang , et al. \"A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro.\" Nucleic Acids Research 3(2004):1197.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003ePfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution : 5 U\/ul Pfu DNA Polymerase, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C \u003cbr\u003e10* Reaction Buffer: 200 mM Tris-HCl (pH 8.8) , 20 mM MgSO4, 100 mM KCl, 100 mM (NH4) 2SO4, 1% Triton X-100, 1 mg\/ml nuclease-free BSA\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eNote: 1. It is critical to withhold Pfu DNA Polymerase until after the addition of dNTPs; otherwise, the proofreading activity of the polymerase may degrade the primers, resulting in nonspecific amplification and reduced product yield.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e 2. Assemble on ice.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41678289469515,"sku":"UA070083-250U","price":140.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678289502283,"sku":"UA070083-1000U","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e61b89182b134eb689f07dd99ad7b5d0.png?v=1787968984"},{"product_id":"recombinant-enterokinase-high-specific-activity","title":"Recombinant Enterokinase (High specific activity)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003erBoEnterokinase,Enteropeptidase,ENTK,PRSS7,hEK\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e26 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Tris-HCl, 200 mM NaCl, 2mM CaCl2, 50% Glycerol, pH 7.4 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Melicherová, Kristína,Krahulec, Ján,?afránek, Martin,et al.Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris[J].Appl Microbiol Biotechnol, 2017, 101(5):1927-1934.\u003cbr\u003e2. Gasparian M E , Ostapchenko V G , Schulga A A ,et al.Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli[J].Protein Expression \u0026amp; Purification, 2003, 31(1):133-139.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eEnterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. This product is a high-purity, high-specific activity enterokinase prepared using a recombinant E.coli. It boasts a broad range of applicability (operating temperature: 4-45°C; pH range: 4.5-9.5) and retains partial activity in the presence of various detergents and denaturants.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e5 U\/μl hEK, 20mM Tris-HCl, 200 mM NaCl, 2mM CaCl2, 50% Glycerol, pH 7.4 @ 25°C \u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eOptimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:\u003cbr\u003e1、Combine 500 ug of sample with reaction buffer *（Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)）\u003cbr\u003e2、Add 1 U of Enterokinase，\u003cbr\u003e3、Incubate at 25°C for 16 hours\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e   Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500U","offer_id":41678289633355,"sku":"UA070084-500U","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678289666123,"sku":"UA070084-1000U","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/107591f3b0d74d9c99a6ce36385e11e2.png?v=1787968986"},{"product_id":"mrna-cap-2-o-methyltransferase","title":"mRNA Cap 2'-O-Methyltransferase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emRNA Cap 2'-O-Methyltransferase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e40 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol (pH 8.0 @ 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Decombe A , El Kazzi P , Decroly E .Interplay of RNA 2'-O-methylations with viral replication[J].Current Opinion in Virology, 2023.\u003cbr\u003e[2] Pastore B , Hertz H , Price I ,et al. pre-piRNA trimming and 2'-O-methylation protect piRNAs from 3' tailing and degradation in C.elegans.[J].Cell reports, 2021, 36(9):109640.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe mRNA Cap 2´-O-methyltransferase is a recombinant protein derived from vaccinia virus, it can add a methyl group to the 2'-O at the 5' end of the RNA immediately adjacent to the first nucleotide of the cap structure. This enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped Cap 0 to Cap 1. Cap 1 structure can enhance the translation efficiency of mRNA and reduce the immunogenicity of mRNA structure itself, so it helps to improve the expression level of encoded protein after mRNA transfection. \u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 50 U\/μl mRNA Cap 2'-O-Methyltransferase in 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol (pH 8.0 @ 25°C)\u003cbr\u003e10* Capping Buffer: 500 mM Tris-HCl, 50mM KCl, 10 mM MgCl2, 10mM DTT (pH 8 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Combine uncapped RNA and nuclease-free water in a final volume of 14.0 μl. \u003cbr\u003e2. Heat at 65°C for 5 minutes.\u003cbr\u003e3. Place tube on ice for 5 minutes.\u003cbr\u003e4. Add the following components in the order specified:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 654.34px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 417.6px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 236.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDenatured uncapped RNA (from above)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e14.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10* Capping Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eGTP (10 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSAM (4 mM, dilute 32 mM stock to 4 mM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVaccinia Capping Enzyme (10 U\/μl)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003emRNA Cap 2´-O-Methyltransferase (50 U\/μl)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.0 μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eNote: Use of RNase Inhibitor is recommended to enhance stability of RNA in the reaction. Add 0.5 μl of RNase Inhibitor during reaction set up. Subtract the additional volume from the amount of H2O used in the reaction.\u003cbr\u003e5. Incubate at 37°C for 60 minutes (For RNA less than 200 nt long increase incubation time to 2 hours).\u003cbr\u003e6. Proceed with purification of the RNA (if required) for downstream applications.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. The capping reaction efficiency is affected by the structure of the RNA 5' end, so it is recommended to open the advanced structure of the RNA 5' end by thermal denaturation (heating at 65°C for 5 min, placing on ice for 5 min). Denaturation conditions can be adjusted according to the structural complexity of the 5' end of RNA. If the 5' end has no advanced structure, this step can be omitted.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. The capping reaction can generally be completed within 60 min. If the RNA 5' end structure is complex or the RNA length is short (≤ 200 nt), the reaction time can be extended to 120 min.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3. SAM is unstable at pH 7-8, 37°C and needs to be freshly configured before the reaction starts. To avoid SAM degradation, the working solution needs to be stored on ice.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e4. RNase inhibitors can be added to the reaction system to prevent RNase contamination, and the recommended concentration is 1-2 U\/μl.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e5. The Cap1 capped RNA of this product can add a Poly(A) sequence at the 3' end through Poly(A) polymerase to form a complete mRNA, which can be used for subsequent transfection experiments or in vitro translation experiments.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"2KU","offer_id":41678298316875,"sku":"UA070092-2KU","price":95.0,"currency_code":"USD","in_stock":true},{"title":"10KU","offer_id":41678298349643,"sku":"UA070092-10KU","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c7cffae8a270424882ac32095beeb4ff.png?v=1787979806"},{"product_id":"pngase-f-ii","title":"PNGase F II","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePeptide-N-glycosidase,Peptide-N-glycosidase F \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1.0 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e62kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% Glycerol, (pH 7.5 @ 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Sun G , Yu X , Bao C ,et al. Identification and characterization of a novel prokaryotic peptide: N-glycosidase from Elizabethkingia meningoseptica.[J].Journal of Biological Chemistry, 2015, 290.\u003cbr\u003e[2] Wang T ,Cai, Zhi P ,et al. Discovery and characterization of a novel extremely acidic bacterial N-glycanase with combined advantages of PNGase F and A.[J].Bioscience Reports, 2014.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eN-glycoprotein deglycosylase (peptide∶N-glycanase, abbreviated PNGase), widely distributed in prokaryotes and eukaryotes, hydrolyzes asparagine (Asn)-linked oligosaccharides on polypeptides and releases intact oligosaccharide chains. Prokaryotic PNGases are found only in Elisabethella meningitidis septica, and there are two PNGases in total, named PNGase F and PNGase F-II. PNGase F-II, while having the function of PNGase F, hydrolyzes and releases intact glycan chains containing α-1,3-core fucoidan glycosylated N-glycoproteins of plant and insect origin, and functions in the same way as PNGase A.      \u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 5U\/μL PNGase FII in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% Glycerol (pH 7.5 @ 25°C) \u003cbr\u003e10* PBS: 136 mM NaCl, 2.6mM KCl, 10 mM Na₂HPO₄, 2 mM‌ KH₂PO₄ (pH 7.4 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eDenaturing Reaction Conditions:\u003cbr\u003e1. Combine 1-5 µg of glycoprotein, 1µl of 10*PBS and H2O (if necessary) in a total reaction volume of 10 µl.\u003cbr\u003e2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.\u003cbr\u003e3. Chill denatured glycoprotein on ice and centrifuge 10 seconds.\u003cbr\u003e4. Make a total reaction volume of 20 µl by adding1-2 µl PNGase F II,1 µl of PBS(10X)and 7-8µl H2O.\u003cbr\u003e5. Incubate reaction at 37°C for 12-16 hour.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1.Reactions may be scaled-up linearly to accommodate larger reaction volumes.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3. We suggest using recombinant Avidin from maize, or horseradish peroxidase (HRP) as positive controls.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e4. Glycoprotein denaturation must not contain SDS, which completely inhibits the enzymatic activity of PNGase F II.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 1 µg of denatured recombinant HRP in 16 hour at 37°C in a total reaction volume of 10 µl\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"20μg","offer_id":41678298447947,"sku":"UA070094-20μg","price":280.0,"currency_code":"USD","in_stock":true},{"title":"100µg","offer_id":41678298480715,"sku":"UA070094-100µg","price":1120.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c787f2ab43794d6d8b62917786408f79.png?v=1787979810"},{"product_id":"recombinant-enterokinase-his-tag-high-specific-activity","title":"Recombinant Enterokinase, His tag (High specific activity)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEnteropeptidase, ENTK, hEK, hEK-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e28 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Melicherová, Kristína,Krahulec, Ján, afránek, Martin, et al. Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris[J]. Appl Microbiol Biotechnol, 2017, 101(5):1927-1934. \u003cbr\u003e2. Gasparian M E, Ostapchenko V G, Schulga A A, et al. Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli[J]. Protein Expression \u0026amp; Purification, 2003, 31(1):133-139. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eEnterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. This product is a high-purity, high-specific activity enterokinase prepared using a recombinant E.coli. It boasts a broad range of applicability (operating temperature: 4-45°C; pH range: 4.5-9.5) and retains partial activity in the presence of various detergents and denaturants.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eStorage Solution: 5 U\/μL hEK-His in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 , 50% Glycerol(pH 7.4 @ 25°C)\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eOptimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:\u003cbr\u003eCombine 500 ug of sample with reaction buffer\u003cbr\u003eRecommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)\u003cbr\u003eAdd 1 U of Enterokinase light chain\u003cbr\u003eIncubate at 25°C for 16 hours\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500U","offer_id":41678298546251,"sku":"UA070095-500U","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678298579019,"sku":"UA070095-1000U","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/aa21fae243cc4d1f9893abc38ac11613.png?v=1789110054"},{"product_id":"hot-start-taq-dna-polymerase","title":"Hot Start Taq DNA Polymerase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHot Start Taq DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e138 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTags \u0026amp; Cleavage sites\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMBP\u0026amp;DDDK\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag, MBP Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% (v\/v) Nonidet P40, 0.5% (v\/v) Tween 20 and 50% (v\/v) glycerol, pH 8.0@ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Kellogg D E, Rybalkin I, Chen S, et al. TaqStart antibody(TM): 'Hot start' PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase[J].BioTechniques, 1994, 16(6):1134-1137.\u003cbr\u003e[2] Obradovic D, Kevresan S Optimization of PCR in application of hot start Taq DNA polymerase for detection of Erwinia amylovora with primers FER1-F and FER1-R1[J]. Microbiology, 2010, 79(6):816-821. DOI: 10.1134\/ S0026261-          710060147.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eHot Start Taq DNA Polymerase is a hot-start Taq enzyme obtained by mixing Taq antibody and Taq DNA polymerase in a certain proportion, which has higher stability and detection rate. Based on the thermal stability of Taq antibody, Hot Start Taq DNA Polymerase can still maintain strict sealing at 45℃, so that non-specific amplification is inhibited during the mixed sample and system heating stage. When the reaction is maintained at 95℃ for more than 30 seconds, Taq antibody is inactivated, and the activity of Taq enzyme is completely released, ensuring that the PCR system has high amplification sensitivity and specificity. Hot Start Taq DNA Polymerase activation is not affected by factors such as buffer pH and ionic strength, and is suitable for various hot-start PCR and qPCR reactions based on Taq DNA polymerase. It is commonly used to amplify low-copy genes from complex templates (genome, cDNA), and is based on PCR\/qPCR molecular diagnostic reagents using hot-start Taq enzyme.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eUA070096: 5 U\/μL Hot Start Taq DNA Polymerase, Taq antibody, 20 mM Tris-HCl (pH 8.0@ 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% (v\/v) Nonidet P40, 0.5% (v\/v) Tween 20 and 50% (v\/v) glycerol\u003cbr\u003e10* Reaction Buffer: 100mM Tris-HCl (pH9.0 @25℃), 500mM KCl, 1% Triton® X-100,15mM MgCl2。\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003ea. Dissolve and mix the various solutions required for the PCR reaction. Due to the hot start nature of the enzyme, reactions can be assembled on the bench at room temperature and transferred to a thermocycler. No separate activation step is required to release the antibody from the enzyme.\u003cbr\u003eb. Refer to the table below to set up the PCR reaction system on the ice bath (if there are multiple similar PCR reactions, you can first prepare a large volume mixture containing water, buffer, dNTP, and Hot Start Taq DNA Polymerase, and then divide it into each PCR reaction tube. According to the situation, sometimes the mixture can include primers):\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 678.34px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 286.6px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 191.002px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 200.007px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10X PCR Buffer (with Mg2+)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003edNTP mix, 10 mM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e200 µM each\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 µM Forward Primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.2 µM (0.05–1 µM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 µM Reverse Primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1\u003c\/font\u003e µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.2 µM (0.05–1 µM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTemplate DNA  \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003evariable\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10 pg-1 μg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eHot Start Taq DNA Polymerase (5 U\/µl)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.25 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.25 U\/50 µl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 397.593px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eDEPC-treated Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eUp to 50µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 397.604px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e-\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eNotes: Gently mix the reaction. Collect all liquid to the bottom of the tube by a quick spin if necessary. Overlay the sample with mineral oil if using a PCR machine without a heated lid.\u003cbr\u003ec. Transfer PCR tubes from ice to a PCR machine with the block preheated to 95°C and begin thermocycling.\u003cbr\u003eThermocycling conditions for a routine PCR:\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 752.34px; height: 326.289px;\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 52.6551px;\"\u003e\n\u003ctd style=\"width: 235.6px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eStep\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 154.009px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTemperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 192.005px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eTime\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 170.002px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eNumber of Cycles\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 51.7245px;\"\u003e\n\u003ctd style=\"width: 298.183px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1.Initial Denaturation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e95°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.229px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 117.72px;\"\u003e\n\u003ctd style=\"width: 298.183px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2.Denaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3.Annealing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4.Extension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e95°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e45–68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e15-30 seconds\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e15-60 seconds\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 minute\/kb\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 298.229px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e30 cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 51.7245px;\"\u003e\n\u003ctd style=\"width: 298.183px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5. Final Extension\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e68°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.229px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 51.7245px;\"\u003e\n\u003ctd style=\"width: 298.183px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e6.Soak\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.194px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eIndefinite\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 298.229px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1 cycle\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. Since the PCR reaction is very sensitive and can amplify the target gene sequence more than 10 million times, please pay attention to avoid contamination of the small amount of DNA to be amplified when using Hot Start Taq Polymerase, and try to consider setting a blank control without a template to confirm whether there is contamination of the DNA to be amplified.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. 2mM Mg2+ can meet the needs of most PCR amplification, and for some PCR, it can be adjusted to 2-4mM to ensure better amplification.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003e1 unit refers to the amount of enzyme required to incorporate 15 nmol of dNTP into acid insoluble substances in 30 minutes at 75°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250U","offer_id":41678312931403,"sku":"UA070097-250U","price":70.0,"currency_code":"USD","in_stock":true},{"title":"1000U","offer_id":41678312964171,"sku":"UA070097-1000U","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/65cfb1db23284424989a3af54fd7f0e8.png?v=1787997809"},{"product_id":"recombinant-trypsin","title":"Recombinant Trypsin","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTrypsin(pig),Porcine pancreas,Recombinant Trypsin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e24 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e2mM HCl，2mM Ca2+，ph3.0  @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eRecombinant trypsin should be dissolved by adding an appropriate amount of 2 mM HCl. The resulting solution should be at an appropriate concentration.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Kunitz,M. Crystalline soybean trypsin inhibitor. 2. General properties. J. Gen. Physiol. 30: 291-307[J]. Journal of General Physiology, 1947, 30(4):291-310.\u003cbr\u003e2. Olsen, J. V . Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues[J].Molecular \u0026amp; Cellular Proteomics, 2004, 3(6):608-614.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eTrypsin specifically cleaves peptide bonds at the carboxyl side of the amino acids lysine and arginine, making it highly effective in breaking down proteins into smaller peptides. This specificity is due to the structure of its active site, which accommodates these positively charged amino acids. The enzyme operates optimally at a slightly alkaline pH, which is consistent with the pH environment of the small intestine.\u003cbr\u003eIn addition to its digestive function, trypsin has significant applications in biotechnology and research. It is commonly used in cell culture to dissociate adherent cells from surfaces, a process known as trypsinization. Furthermore, trypsin is employed in proteomics for protein digestion prior to mass spectrometry analysis, enabling the identification and characterization of proteins.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eRecombinant trypsin lyophilized powder\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is a recombinantly expressed porcine trypsin that is free from animal-derived components. It is suitable for applications in cell culture, cell fermentation, enzymatic hydrolysis of proteins, tissue dissociation, and insulin production. The exact dosage should be optimized based on specific experimental conditions.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Storage buffer:2mM HCl, pH3.0.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eIn a 3.2ml reaction system using BAEE as substrate, the UV absorption at 253nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41678324400203,"sku":"UA070111-1mg","price":35.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41678324432971,"sku":"UA070111-5mg","price":45.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0335f655ef634860bf472e771c6b3089.png?v=1788008407"}],"url":"https:\/\/www.antbioinc.com\/collections\/enzyme.oembed?page=6","provider":"AntBio","version":"1.0","type":"link"}