{"title":"Cytokine Sets","description":null,"products":[{"product_id":"th2-cytokine-set-human","title":"Th2 Polarization Kit, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e \u003ctbody\u003e\u003ctr\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eIL-2  Protein, Human\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.5pt\"\u003eIL-4  Protein, Human\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"color:#333333\"\u003eReconstitute  at 0.1-1 mg\/ml according to the size in ultrapure water after rapid  centrifugation.\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e \u003c\/tr\u003e\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e \u003ctbody\u003e\n\u003ctr\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eIL-4 Protein, Human \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eIL-2 Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eanti-human CD3 mAb\u003c\/span\u003e  \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eanti-human CD28 mAb\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eanti-huamn IL-4 mAb\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003eanti-Human IFN-γ mAb\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003e-20 to -80℃. Please avoid  repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003eValid for 12 months from the date of receipt.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e  \u003c\/td\u003e \u003c\/tr\u003e  \u003ctr\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eβ-Molecular Biology\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd\u003e  \u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003e2-8°C\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt;color:#333333\"\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e \u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border:solid windowtext 1.0pt;width:501.55pt;\" border=\"1\" cellspacing=\"3\" cellpadding=\"0\" width=\"669\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:89.45pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eFormulation\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10ml System\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e（\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eS Size\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100ml System\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e（\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eM Size\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:89.45pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1000ml System\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e（\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eL Size\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eIL-2 Protein, Human\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLyophilized Powder\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e40ng \/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eIL-4 Protein, Human\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLyophilized Powder\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e30ng\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e30\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.25pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eanti-human CD3 mAb\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";font-size:11.0pt;'\u003e \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eµg\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eanti-human CD28 mAb\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";font-size:11.0pt;'\u003e \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eµg\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.25pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eAnti-human IL-4 mAb\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100ug\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.25pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eAnti-Human IFN-\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eγ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e mAb\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100ug\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:29.8pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10mg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:59.65pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:.75pt .75pt .75pt .75pt;width:175.45pt;\" width=\"234\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eβ-Molecular Biology\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e（\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e14.3 M (pure liquid)\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:0cm 0cm 0cm 0cm;width:75.7pt;\" width=\"101\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Microsoft YaHei UI\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:.75pt .75pt .75pt .75pt;width:55.0pt;\" width=\"73\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e55\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003el\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003el\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:59.65pt;padding:.75pt .75pt .75pt .75pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003el\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003eNote:Some reagents may have leftovers.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:9.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eTh2 Polarization Kit, human - Protocol:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e1. Coating with Anti-human CD3 mAb for T cell Activiation.\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.1 Dilute the anti-human CD3 mAb to a working concentration (5 µg\/mL) using sterile PBS.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.2 Add 1 mL of the diluted anti-human CD3 mAb to each well of a 24-well cell culture plate for coating.  Incubate the plate at 37°C for 2 hours, or at 4°C overnight.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e2. Cell Seeding and Initiation of Th2 Polarization\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eT cell polarization the cells should be resuspended in culture medium. .Both the dilution and the cell density are important to assure optimal stimulation and cell growth.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.1 The next day, aspirate the coating solution and gently wash\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eeach well 2-3 times with ice-cold sterile PBS to remove unbound antibody.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.2 Resuspend human CD4+ T cells sorted with CD4 Nanobeads, human (Cat. No.: S0B5740) in Th2 polarization\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003ecomplete medium at a concentration of 1×10⁶ cells\/mL. (Or alternative: S0K1014, Human Naïve CD4⁺ T Cell Isolation Kit)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eTh2 polarization Complete medium formulation: RPMI-1640 basal medium, supplemented with 10% FBS, 55 μM β-mercaptoethanol, 200 U\/mL recombinant human IL-2, 30 ng\/mL recombinant human IL-4, 10 μg\/mL anti-human IFN-γ neutralizing antibody and 1 μg\/mL anti-human CD28 mAb.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.3 Seed 1 mL of the cell suspension into each pre-coated 24-well plate. Incubate the plate in a cell culture incubator at 37°C with 5% CO₂.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e3.Culture Maintenance and Cell Expansion\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3.1 After 48 hours of culture, gently resuspend and collect the cell suspension. Centrifuge the cell suspension at 300 × g for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3.2Following removal of the supernatant, resuspend the cell pellet in fresh complete Th2 polarization medium. Adjust the cell density to 3–5 × 10⁵ cells\/mL and re-plate the cells into new culture vessels for further expansion. For larger-scale cultures, cells may be seeded into 6-well plates at a volume of 2–3 mL per well.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3.3Thereafter, monitor cell density and morphology every 12 hours using an inverted microscope. By day 4, cell clusters aggregating in the center of the wells should be observable. At this point, supplement each well with an equal volume of fresh complete Th2 polarization medium, and replenish the corresponding cytokines and antibodies to their appropriate working concentrations to maintain optimal growth conditions.\u003cstrong\u003e4. Cell Restimulation and Intracellular Cytokine Capture\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eOn day 5 of culture, harvest the cells and wash once with ice-cold PBS. Resuspend the cells in RPMI-1640 complete medium containing 10 ng\/mL PMA, 1 µg\/mL Ionomycin, and 10 µg\/mL Brefeldin A. Adjust the cell density to 1×10⁶ cells\/mL and incubate in a humidified incubator at 37°C with 5% CO₂ for 5 hours in the dark for restimulation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:9.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eNegative Control Group (Th0) Setup\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eTo evaluate the specificity of polarization, set up a Th0 negative control group in parallel. The procedure is identical to the Th1 induction group described above, except for the use of a different culture medium.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.Th0 Maintenance Medium: RPMI-1640 supplemented with 10% FBS, 55 μM β-mercaptoethanol, 10 μg\/mL anti-human IL-4, 10 μg\/mL anti-human IFN-γ, 200 U\/mL IL-2, and 1 μg\/mL anti-human CD28 mAb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2. Absence of Polarizing Cytokines: This medium does not contain any polarizing cytokines.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3. The negative control group did not require treatment with PMA, Ionomycin, and Brefeldin A for 5 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eExcept for the above differences, cell seeding, medium change, and density monitoring were the same as those in the Th2 induction group.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:9.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eFlow Cytometry Detection\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e1.Cell Harvesting:\u003c\/strong\u003e Discard the culture supernatant following induction. Wash the cells once with phosphate-buffered saline (PBS). Discard the wash supernatant and gently resuspend any adherent cells in a fresh volume of PBS by pipetting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e2.Cell Counting: \u003c\/strong\u003eCount the cells using an automated cell counter and calculate the total cell number. Centrifuge the cell suspension at 300 × g for 5 minutes at 4°C. Discard the supernatant and resuspend the cell pellet at a concentration of 1×10⁷ cells\/mL in PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e3.Viability Staining: \u003c\/strong\u003eAdd 100 µL of the diluted Fixable Viability Dye 452 (Cat# S0D0021) per 1×10⁶ cells. Refer to the product manual for specific dilution instructions. Incubate as recommended by the manufacture.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e4.Cell Washing:\u003c\/strong\u003e Wash the cells by adding an excess volume of PBS. Centrifuge at 300 × g for 5 minutes at 4°C and carefully decant the supernatant to remove residual dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e5.Cell Fixation: \u003c\/strong\u003eTransfer 100 µL of cell suspension (containing 1×10⁶ cells) to each well of a 96-well U-bottom plate. Centrifuge the plate at 300 × g for 5 minutes at 4°C and discard the supernatant by rapid inversion. Fix cells by adding 200 µL of 4% paraformaldehyde (PFA) per well. Incubate for 15 minutes at room temperature, protected from light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e6.Cell Washing: \u003c\/strong\u003eCentrifuge the plate at 300 × g for 5 minutes at 4°C and discard the supernatant. Add 200 µL of PBS to each well to wash. Repeat centrifugation and discard the supernatant to ensure complete removal of residual fixative.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e7.Cell Permeabilization: \u003c\/strong\u003eAdd 200 µL of BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Solution to each well. Incubate for 30 minutes at 4°C. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e8.Cell Blocking: \u003c\/strong\u003eAdd 100 µL of 1× BD Perm\/Wash™ Buffer per well. Include an appropriate concentration of Human IgG (e.g., 0.5-1 µg\/µL) in this buffer for Fc receptor blocking. Incubate for 10-15 minutes at 4°C. Centrifuge at 300 × g for 5 minutes at 4°C and discard the supernatant. Note: Step 8 can be performed concurrently with Step 7 by adding the blocking agent directly to the permeabilization solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e9.Antibody Incubation: \u003c\/strong\u003eAdd 100 µL of 1× BD Perm\/Wash™ Buffer per well, followed by the appropriately diluted PE\/Dazzle™ 594 anti-human IL-4 Antibody  according to the manufacturer's instructions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e10.Cell Washing: \u003c\/strong\u003eCentrifuge the plate at 300 × g for 5 minutes at 4°C and discard the supernatant to remove unbound antibody. Add 200 µL of PBS to each well for a final wash. Centrifuge again and discard the supernatant. Resuspend the final cell pellet in 100-200 µL of PBS or a dedicated flow cytometry staining buffer for acquisition.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e11.Flow Cytometric Acquisition: \u003c\/strong\u003eAcquire samples on a flow cytometer.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003estore in separate containers to reduce the number of freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519872802891,"sku":"UA090018-S","price":220.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":41519872835659,"sku":"UA090018-M","price":550.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519872868427,"sku":"UA090018-L","price":2500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0f72530075e84eaeab1d3ff5c3ab6979_0493b2eb-6862-416c-86d8-cc1c07616c60.jpg?v=1792753288"}],"url":"https:\/\/www.antbioinc.com\/collections\/cytokine-sets.oembed","provider":"AntBio","version":"1.0","type":"link"}