{"title":"Auxiliary Reagent","description":"","products":[{"product_id":"baculovirus-quantitative-titration-kit","title":"Baculovirus Quantitative Titration Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBaculovirus qPCR Quantitative Titration Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNotRelated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eThe Baculovirus Quantitative Titration Kit serves as an extraction and titration system for fresh (less than 3 months old) budded baculovirus stocks based on AcMNPV baculoviral vectors. Ultra-sensitive TaqMan qPCR technique and accurate titrated virus stocks are employed in this kit, providing an alternatively time-saving method of titration up to 2~3 hours. Therefore, reliable multiplicity of infection (MOI) can be determined real-timely, substituting traditional plaque assays or antibody-based assays which usually take 3~4 days.\u003c\/p\u003e\n\u003cp\u003eThe primers and probe included in this kit are specific to \u003cem\u003egp64\u003c\/em\u003e that encodes the essential envelope protein GP64, and the sequences, concentration and ration are also optimized. Virus Lysis Buffer from this kit can degrade viral protein, releasing viral DNA that can be used directly as template for qPCR without further purification. Virus Standard, Positive Control (PC) and negative template control (NTC) should be set up every time of quantitative titration.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable width=\"100%\" border=\"2\" style=\"border-collapse: collapse; width: 73.0994%; height: 398px;\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 42px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 42px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 42px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 42px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 42px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 42px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eVirus Standard\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 42px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eAcMNPV Virus, 2*10\u003csup\u003e8\u003c\/sup\u003e pfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 42px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 42px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 42px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eVirus Lysis Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 42px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 42px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 42px;\"\u003e\n\u003ctd style=\"text-align: center; height: 42px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eProteinase K*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 42px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e10x, 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 42px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 66px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 66px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003ePrimer \u0026amp; Probe Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 66px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eForward Primer \u0026amp; Reverse Primer \u0026amp; Probe, 240μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 66px; width: 26.0796%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eIN THE DARK\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 66px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 66px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eqPCR Low ROX mix\u003csup\u003e†\u003c\/sup\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 66px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1.2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 66px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 66px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 66px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eDNA Positive Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 66px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e20ng\/μL, 25 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 66px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 42px;\"\u003e\n\u003ctd width=\"147\" style=\"text-align: center; height: 42px; width: 28.3948%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eRNase-free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"235\" style=\"text-align: center; height: 42px; width: 45.3964%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1.5mL*3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"135\" style=\"text-align: center; height: 42px; width: 26.0796%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e-20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e* Add Proteinase K to Virus Lysis Buffer before use according to the number of your samples.\u003c\/p\u003e\n\u003cp\u003e† Compatible to ABI Prism 7500\/7500 Fast, QuantStudio 3\/5\/6 Flex\/7 Flex System, ViiA\u003csup\u003eTM\u003c\/sup\u003e 7 System,\u003c\/p\u003e\n\u003cp\u003eStratagene Mx3000\/Mx3005P, Corbett Rotor Gene 3000, et. al.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdditional Materials Required\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eQuantitative real-time PCR Thermocycler\u003c\/li\u003e\n\u003cli\u003eOptical 96-well PCR plates or 8-well PCR strips with strip caps.\u003c\/li\u003e\n\u003cli\u003eCentrifuge for tubes, PCR strips or PCR plates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eBaculoviral Titration Protocol\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Centrifuge the medium from baculovirus-infected cells at 500g for 5mins. Transfer supernatant to a new 1.5 mL tube, discard cells and debris.\u003c\/p\u003e\n\u003cp\u003e2. Dilute Virus Standard serially using the RNase-free water to generate standard serial log dilutions containing 6 points, 2*10\u003csup\u003e8\u003c\/sup\u003epfu\/mL, 2*10\u003csup\u003e7\u003c\/sup\u003epfu\/mL, 2*10\u003csup\u003e6\u003c\/sup\u003epfu\/mL, 2*10\u003csup\u003e5\u003c\/sup\u003epfu\/mL, 2*10\u003csup\u003e4\u003c\/sup\u003epfu\/mL, 2*10\u003csup\u003e3\u003c\/sup\u003epfu\/mL.\u003c\/p\u003e\n\u003cp\u003e3. Dilute your unknown samples, if you suspect the titer to be great than 10\u003csup\u003e8\u003c\/sup\u003epfu\/mL.\u003c\/p\u003e\n\u003cp\u003e4. Take 80μL standard serial dilutions and your unknown diluted (e.g., 1 in 5 or 1 in 10) or undiluted samples to PCR tubes, respectively. Centrifuge at 13000rpm for 5min, and then discard supernatant.\u003c\/p\u003e\n\u003cp\u003e5. Re-suspend the virus pellet in 20μL Virus Lysis Buffer (add final 1x Proteinase K before use)in tips.\u003c\/p\u003e\n\u003cp\u003e6. Run the following lysis program to extract virus DNA. The lysis can be used as templates for qPCR.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 33.584%;\" height=\"338\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eTemperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eTime\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e55℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e30min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e95℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e5min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e55℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e4min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e95℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e55℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e95℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e30s\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"60\" style=\"text-align: center; width: 26.058%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eStep 7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"101\" style=\"text-align: center; width: 47.4068%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e20℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"57\" style=\"text-align: center; width: 26.086%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eHold\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e7. Prepare qPCR reactions on ice. Multiply the following amounts by the number of reactions including 6 standard serial dilutions, unknown samples, 1*positive control and 1*NTC. Triplicate is recommended for every sample.\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 25.3133%;\" height=\"215\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"184\" style=\"text-align: center; width: 68.7017%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"85\" style=\"text-align: center; width: 30.9115%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003e20 µL\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"184\" style=\"text-align: center; width: 68.7017%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eqPCR mix（2X）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"85\" style=\"text-align: center; width: 30.9115%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e10µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"184\" style=\"text-align: center; width: 68.7017%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003ePrimer\u0026amp;Probe Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"85\" style=\"text-align: center; width: 30.9115%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"184\" style=\"text-align: center; width: 68.7017%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eTemplate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"85\" style=\"text-align: center; width: 30.9115%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"184\" style=\"text-align: center; width: 68.7017%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eRNase-free water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"85\" style=\"text-align: center; width: 30.9115%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eTo 20 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e8. PCR plate diagram\u003c\/p\u003e\n\u003ctable width=\"78%\" border=\"1\" style=\"border-collapse: collapse; width: 85.5473%;\" height=\"336\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e8\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e8\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e8\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e7\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e7\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e7\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e6\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e6\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e6\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e5\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e5\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e5\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e4\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e4\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e4\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e3\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e3\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2*10\u003csup\u003e3\u003c\/sup\u003epfu\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eSample6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003ePC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003ePC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003ePC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eNTC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eNTC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003eNTC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"16%\" style=\"text-align: center; width: 16%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e…\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e9. Place the 96-well plate or PCR strips in the Quantitative real-time PCR Thermocycler. Run the following qPCR program to acquire FAM fluorescent intensity.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable height=\"154\" border=\"1\" style=\"border-collapse: collapse; width: 37.2694%; height: 154px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"75\" style=\"width: 45.9327%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eTemperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"73\" style=\"width: 33.73%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eTime\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"42\" style=\"width: 20.0364%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e\u003cstrong\u003eCycle\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"75\" style=\"width: 45.9327%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e95℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"73\" style=\"width: 33.73%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e2min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"42\" style=\"width: 20.0364%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e1x\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"75\" style=\"width: 45.9327%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e95℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"73\" style=\"width: 33.73%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e5s\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"2\" width=\"42\" style=\"width: 20.0364%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e40x\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"75\" style=\"width: 45.9327%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e60℃*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"73\" style=\"width: 33.73%; text-align: center;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 12px;\"\u003e5s\u003csup\u003e#\u003c\/sup\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e*Acquire FAM fluorescence data.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e#Alternatively, set as the minimal time allowed by your Quantitative real-time PCR thermocycler.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e\u003cstrong\u003eData Analysis\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e1. Once the qPCR program is finished, the Ct values will generally be calculated in the default threshold (example shown in figure 1).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e2. Export the Ct values into Microsoft Excel or other data analysis software. Determine average Ct Values for each triplicate samples. All Ct values should be below that of the NTC.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e3. Generate a standard curve to demonstrate linear correlation between Ct values and the standard titer (log scale), like example shown in figure 2. R\u003csup\u003e2\u003c\/sup\u003e is required to above 0.98, while PCR efficiency 0.90~1.10.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e4. Plot the unknown samples Ct values against linear correlation formulation to obtain a equivalent pfu\/mL value for each sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e\u003cimg src=\"https:\/\/univ-shop.oss-cn-shanghai.aliyuncs.com\/pc\/images\/20230511\/38565432cd974a749a5fbef5d3759ac7.png\" width=\"237\" height=\"147\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003eFigure 1. Amplification plots of the fluorescence intensity against the cycle with horizontal threshold line.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003e\u003cimg src=\"https:\/\/univ-shop.oss-cn-shanghai.aliyuncs.com\/pc\/images\/20230511\/651d47d64c984d5487b294ffa1308620.png\" width=\"379\" height=\"125\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 16px;\"\u003eFigure 2. The standard correlation curve between Ct values and the standard titer (log scale), followed calculated data on the right.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100Tests","offer_id":41293285359691,"sku":"UA070018-100Tests","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_ea6f403b-9e39-4f36-bfef-ed066f123033.png?v=1789110197"},{"product_id":"dt3c-diphtheria-toxin-spg-3c-domain-protein-corynephage-beta","title":"DT3C (Diphtheria toxin \u0026 spg 3C domain) Protein, Corynephage beta","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eDT3C,Diphtheria toxin \u0026amp; spg 3C domain\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP00588、P19909\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eGly33-Lys417\u0026amp;\u0026amp;ILe291-Glu497\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e65kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE and HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Tris, 200mM NaCl, pH8.0\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e·12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e·1 month, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e·Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eBiochem Biophys Res Commun. 2014 Nov 28; 454(4):600-3.Epub 2014 Nov 1.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eDT3C consists of diphtheria toxin (DT) lacking the receptor-binding domain but containing the C1, C2, and C3 domains of Streptococcus protein G (3C). When a mAb–DT3C conjugate, which functions in vitro like an ADC, reduces the viability of cancer cells, the mAb being tested must have been internalized by the target cells. DT3C can thus be a tool to identify efficiently and easily mAbs that can be internalized by cells, thereby enhancing the development of promising ADCs.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":41293286604875,"sku":"UA070063-10μg","price":135.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":41293286637643,"sku":"UA070063-50μg","price":300.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41519861760075,"sku":"UA070063-100μg","price":535.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":41519861792843,"sku":"UA070063-500μg","price":1020.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41519861825611,"sku":"UA070063-1mg","price":1635.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3aeed2402dc04ac39a4c7038b304d5bb.png?v=1789110084"},{"product_id":"ubiquitin-rhodamine-110","title":"Ubiquitin Rhodamine 110","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP0CG47.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e8934\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e≥95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eR100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eSolubility in DMSO: 30 mg\/mL (assisted by sonication and heating; hygroscopic DMSO significantly affects the solubility of the product, please use newly opened DMSO)\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(85, 85, 85);font-size: 14px;background-color: rgb(243, 249, 255)\"\u003e\u003cfont class=\"bg-o-color-4\"\u003eStore at -20°C or -80°C   （\u003cspan style=\"color: rgb(122, 122, 122);font-size: 14px\"\u003esealed storage, away from moisture and light\u003c\/span\u003e）\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"50μg","offer_id":41518779170891,"sku":"UA080158-50μg","price":180.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":41518779203659,"sku":"UA080158-500μg","price":1645.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41518779236427,"sku":"UA080158-1mg","price":3300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c7d688ee-0b14-4edc-9a8b-b9d4f4a3d2fa.png?v=1787623273"},{"product_id":"gfp-his-tag-protein-aequorea-victoria","title":"GFP His Tag protein, Aequorea victoria","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAequorea victoria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e GFP Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAAB65663\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSer2-Lys238, with N-terminal 8*His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e27.8kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u0026amp;RP-HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePBS, pH7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Trends Biochem Sci 1995 Nov;20(11):448-55.\u003cbr\u003e2.Biol Chem. 1998 Dec 25;273(52):34970-5.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. It is a widely used reporter in gene expression and protein localization studies. the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a widely used reporter in studies of gene expression and protein localization. GFP is a single chain polypeptide of 238 amino acids .Most of these amino acids form β sheets that are compacted through an antiparallel structure to form the barrel. So far, they have been used as reporters of gene expression, tracers of cell lineage, and as fusion tags to monitor protein localization within living cells.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"50μg","offer_id":41518806007883,"sku":"UA070020-50μg","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41518806040651,"sku":"UA070020-100μg","price":340.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":41518806073419,"sku":"UA070020-500μg","price":1220.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/43b856d4-4b1d-4a32-804f-4845074145f2.png?v=1787634229"},{"product_id":"biotin-labeling-for-avitag-protein","title":"Biotin Labeling for Avitag Protein","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAviTag protein biotinylation kit, biotinylation kit, biotinylation, labeling, bifunctional ligase\/repressor, BirA, biotin-protein ligase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP06709\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eMet1-Lys321\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e35kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e＞95% by SDS-PAGE and HPLC\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.5EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Bicine, 50% Glycerol, pH8.3\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cul\u003e\n\u003cli\u003e12 months from date of receipt, -20℃ as supplied. \u003c\/li\u003e\n\u003cli\u003eStore BirA and BirA Buffer at -20℃.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freezing and thawing cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Howarth M, Ting AY. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nat Protoc. 2008;3(3):534-45.\u003cbr\u003e2.Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA. Methods Mol Biol. 2015;1266:171-84.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eBirA, the most characterized biotin-acetyl-CoA-carboxylase ligase, has been successfully applied to biotin labeling with a wide range of protein substrates that fused an acceptor peptide (AP, also known as Avitag)\u003cem\u003e in vivo \u003c\/em\u003eand\u003cem\u003e in vitro\u003c\/em\u003e. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. BirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. Due to its extremely high strength, the interaction between biotin and streptavidin has been exploited for biotechnological applications, like \u003cem\u003ein vivo\u003c\/em\u003e imaging, cell sorting, \u003cem\u003eet al\u003c\/em\u003e.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cimg class=\"img-fluid\" 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\"\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eRecommendation before Ligation\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1. Higher protein concentration (above 40μM) is always recommended to be biotin labelled. It takes more time to label target protein, if protein concentration is lower.\u003c\/p\u003e\n\u003cp\u003e2. 2.0μg BirA is recommended to ligate D-biotin to 10nmol of Avi tagged protein at 40μM in 30-60 min at 37℃ or 60-90min at RT. Ligation reaction can also be performed at 4℃, but much more time required, like overnight.\u003c\/p\u003e\n\u003cp\u003e3. ATP solution is vulnerable to hydrolyze with time, so longer incubation time may be problematic the target protein from ATP, lowering the ligation efficiency.\u003c\/p\u003e\n\u003cp\u003e4. 10X Buffer is provided\u003cem\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eAdd the following to a microcentrifuge tube:\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 36.6318%; height: 258px;\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003e\u003cstrong\u003eAvi tagged Protein\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003e\u003cstrong\u003e10nmol\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003e10X Buffer A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003e10X Buffer B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003e10X Buffer C\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003eIf necessary\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003eBirA\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003e2.0μg\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 43px;\"\u003e\n\u003ctd width=\"344\" style=\"text-align: center; width: 60.7751%; height: 43px;\"\u003e\u003cp\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"123\" style=\"text-align: center; width: 39.0087%; height: 43px;\"\u003e\u003cp\u003eTo 250μL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e5. Mix and incubate at 37°C. Take 20μl aliquots at 30min, 60min, 90min to analysis.\u003c\/p\u003e\n\u003cp\u003e6. Analyze by Pulldown or other Biotin quantitative kit.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"200μg","offer_id":41518844149835,"sku":"UA070015-200μg","price":185.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41518844182603,"sku":"UA070015-1mg","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/92b711f3b8a74bc0bc45d71b23032243_703829dd-264e-4163-be6a-18426380edb4.png?v=1789902013"},{"product_id":"cholera-toxin-b-subunit-ctb","title":"Cholera Toxin B subunit （CTB)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCHOLERA TOXIN B SUBUNIT、CTB、 Cholera Toxin B subunit、CTxB、Choleragenoid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP01556\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eMTPQNITDLCAEYHNTQIYTLNDKIFSYTESLAGKREMAIITFKNGAIFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMAN\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e11.8kDa(Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; RP-HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.2EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20mM Tris, 100mM NaCl, pH9.0\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at less than 1 mg\/mL according to the size in ultrapure water  after rapid centrifugation .\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, -20 to -70 °C as supplied. \u003cbr\u003e· 6 months, -20 to -70 °C under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  \u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eCholera toxin （CTB）belongs to the AB5 –subunit family oftoxins.The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits(~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport)of the enzymatic component.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500μg","offer_id":41518983020619,"sku":"UA070002-500μg","price":235.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41518983053387,"sku":"UA070002-1mg","price":385.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b06ff509d5ab4aa0995465506544049b.png?v=1789380009"},{"product_id":"ph-sensitive-igg-labeling-reagents-plus","title":"pH-sensitive IgG labeling reagents plus(Green)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eThe product has a MW of 21.8 kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to settie down at the bottom of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 10uL\u003cfont\u003e（10ug package）.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3. After adding sterile water, cover the lid and mix them bygently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eWe recommend storing the vial(s) at -20℃, desiccated and protected from light. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eOnce reconstituted, the reagents can be stored at 2-8℃ for 1~2 weeks, or with 50% glycerol at -20℃.(Avoid repeated freezing and thawing)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eStore at -20'C to -80'C for 12 months in lyophilized form\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003epH sensitive IgG labeling reagents provide an easy solution to measure internalization activities of antibodies. This reagent utilizes a pH-sensitive fluorescently labeled Fc binding protein that binds to IgG antibodies from various species, resulting in the formation of a fluorescently labeled antibody-reagent complex. After antibody internalization, the surrounding pH becomes acidic and significantly enhances fluorescence signal of antibody-reagent complex. The fluorescence intensity can be used as an indicator to determine the internalization activity of antibodies. By measuring the strength of the fluorescence signal, researchers can assess the efficiency of antibody internalization into cells. This information is crucial in understanding the cellular uptake mechanism of antibodies and assessing their efficacy in targeted therapies or diagnostic applications. Additionally, monitoring the fluorescence intensity can also provide insights into the kinetics of antibody internalization, helping researchers optimize experimental condition and improve the design of antibody-based drug delivery systems.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eThis product can becan be used for human IgG1, IgG2, IgG3, IgG4, rabbit IgG, mouse IgG1, IgG2a, IgG2b and IgG3 and can be detected with  Flow cytometry  FITC or AF488 filter.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eUA070080\u003c\/font\u003e \u003cfont\u003efor Detection of antibody\u003c\/font\u003e \u003cfont\u003einternalization-SOP\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1. UA070080\u003c\/font\u003e \u003cfont\u003eincubation with antibodies\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.1 Dissolve UA070080\u003c\/font\u003e \u003cfont\u003ein freeze-dried powder form according to datasheet using\u003c\/font\u003e \u003cfont\u003edeionized water.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.2 Mix the tested antibody with UA070080\u003c\/font\u003e \u003cfont\u003ein a mass ratio of 3.5:1 (molar ratio 1:2),\u003c\/font\u003e \u003cfont\u003eand dilute the antibody with UA070080\u003c\/font\u003e \u003cfont\u003emixture to twice the tested\u003c\/font\u003e \u003cfont\u003econcentration using complete culture medium.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.3 Incubate at room temperature in dark for 1 hour to obtain Ab-UA070080\u003c\/font\u003e \u003cfont\u003ecomplex 2X working solution.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2. Ab-UA070080\u003c\/font\u003e \u003cfont\u003ecomplex incubation with cells\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2.1 Collect and wash cells, adjust cell concentration using complete culture medium.1-2×105 cells\/mL for suspend cells, 0.5-1×105 cells\/mL for adherent cells. Add\u003c\/font\u003e \u003cfont\u003e100μL cell suspension to each hole of the 96 well plate.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2.2 Add 100μL Ab-UA070080\u003c\/font\u003e \u003cfont\u003ecomplex 2X working solution to each hole. Incubate in\u003c\/font\u003e \u003cfont\u003ea 5% CO2\u003c\/font\u003e \u003cfont\u003eincubator at 37℃. \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e3. Flow cytometry detection\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eAfter 18-24h of culture, cells were collected for flow cytometry to detect the entosis effect of antibodies using FITC or AF488 channels\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":41519846850635,"sku":"UA070080-10μg","price":750.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":41519846883403,"sku":"UA070080-50μg","price":2205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/786a8d84-ee3c-4644-b79a-7efc496c3305.png?v=1787673742"},{"product_id":"mbp-68-86","title":"MBP (68-86)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMyelin Basic Protein peptide (68-86); Myelin Basic Protein peptide segment (68-86)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eYGSLPQKSQRSQDENPV\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1933.06\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eReconstitute at 1 mg\/ml according to the size in ultrapure water.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20℃\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eMBP, the full English name Myelin Basic Protein, Chinese name myelin basic protein or myelin basic protein, is an important component of myelin in the central nervous system (CNS), which is synthesized by oligodendrocytes and Schwann cells, and can be used as a marker for these two kinds of cells. MBP is a single chain polypeptide with a molecular weight of about 18.5kDa, located in the dense myelin sheath and nucleus pulposus. It is a potential targeted antigen that can induce experimental allergic encephalomyelitis (EAE) in animals. The encephalitis-causing peptides of MBP varied with different sensitive strains and were related to MHC Class II genotype.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e1.  This product is provided in the form of freeze-dried powder, which may be difficult to observe due to small amounts. Please add the solvent directly into the bottle and swirl at low speed to ensure full dissolution; The prepared storage solution is divided according to the single dosage and frozen at -20℃ away from light to avoid repeated freezing and thawing.\u003cbr\u003e2.  For your safety and health, please wear a lab coat and disposable gloves.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41519862874187,"sku":"UA080404-1mg","price":120.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41519862906955,"sku":"UA080404-5mg","price":170.0,"currency_code":"USD","in_stock":true},{"title":"10mg","offer_id":41519862939723,"sku":"UA080404-10mg","price":300.0,"currency_code":"USD","in_stock":true},{"title":"50mg","offer_id":41519862972491,"sku":"UA080404-50mg","price":535.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_246c5440-a2e3-4734-a764-c4425f8cc2d2.png?v=1789113661"},{"product_id":"plp-139-151","title":"PLP (139-151)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMyelin proteolipid protein (139-151), Proteolipid protein (139-151)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHSLGKWLGHPDKF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1521.74\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eReconstitute at 2 mg\/ml according to the size in ultrapure water.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003ePLP (139-151) is a synthetic myelin proteolipid protein (PLP) fragment. Immunization with this peptide induces severe clinical and histological experimental allergic encephalomyelitis (EAE), an animal model of multiple sclerosis.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41519863201867,"sku":"UA080406-1mg","price":135.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41519863234635,"sku":"UA080406-5mg","price":200.0,"currency_code":"USD","in_stock":true},{"title":"10mg","offer_id":41519863267403,"sku":"UA080406-10mg","price":335.0,"currency_code":"USD","in_stock":true},{"title":"50mg","offer_id":41519863300171,"sku":"UA080406-50mg","price":570.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_82fe87ff-5840-430c-b1d5-0d2793348f29.png?v=1788501758"},{"product_id":"irbp-1-20-human","title":"IRBP (1-20), Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGPTHLFQPSLVLDMAKVLLD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e2194.62\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003eDMSO : 100 mg\/mL (45.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)\u003c\/p\u003e\n\u003cp\u003eWater : 40 mg\/mL (18.23 mM; adjust pH to 8 with NH3·H2O)\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20℃~ -80℃\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"color: rgb(88, 88, 91);font-size: 14px;background-color: rgb(251, 251, 252)\"\u003eThis peptide is a 1 to 20 amino acid fragment of the interphotoreceptor retinoid binding protein (IRBP). IRBP is a 140-kDa glycolipoprotein residing in the interphotoreceptor matrix between the neural retina and the retinal pigment epithelium. Human IRBP peptide 1-20 contains a major epitope for the H-2b haplotype. Immunization with IRBP (1 – 20) induces T-cell–mediated experimental autoimmune uveoretinitis (EAU) disease. The pathology of disease induced by the peptide, or by adoptive transfer of cells specific to the peptide, is similar to that induced by the whole IRBP protein.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003ePlease refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eStorage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eNote: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41519863332939,"sku":"UA080407-1mg","price":120.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41519863365707,"sku":"UA080407-5mg","price":200.0,"currency_code":"USD","in_stock":true},{"title":"10mg","offer_id":41519863398475,"sku":"UA080407-10mg","price":335.0,"currency_code":"USD","in_stock":true},{"title":"50mg","offer_id":41519863431243,"sku":"UA080407-50mg","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9d71af43-0ee8-4153-9e52-65e1945f3a1f.png?v=1788501760"},{"product_id":"ph-sensitive-igg-labeling-reagents-max","title":"pH-sensitive IgG labeling reagents Max(Green)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e31kDa \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4 with 5% trehalose is added as protectants\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10.5pt\"\u003e1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to settie down at the bottom of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10.5pt\"\u003e2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 10uL\u003cfont style=\"font-size: 14px\"\u003e（10ug package）.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10.5pt\"\u003e3. After adding sterile water, cover the lid and mix them bygently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 1 -2week, 2 to 8℃ under sterile conditions after reconstitution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eAntibody internalization labeling probe. It can bind to candidated antibodies to produce soluble complex that only fluoresce in acidic environments for the study of antibody internalization.\u003cbr\u003eThe pH-sensitive Labeling probe binds to your primary antibodies via Fc binding protein to a pH-dependent fluorescent molecular. This fluorescent complex reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. The internalized antibodies are detected by measuring fluorescence intensity of the cells.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003eThis product can becan be used for human IgG1, IgG2, IgG3, IgG4, rabbit IgG, mouse IgG1, IgG2a, IgG2b and IgG3 and can be detected with  Flow cytometry  FITC or AF488 filter.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image image_resized\" style=\"aspect-ratio:1328\/1492;width:55.2%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/19882739\/d3171c43-5f2d-4101-abcd-9c438ad55619.jpg?access_token=8baa4a78-ac08-4e0a-b055-6ef01b0de624\" alt=\"\" width=\"1328\" height=\"1492\"\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e1.   Incubation of UA070122 with Antibody\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 1.1 Reconstitute the lyophilized UA070122 powder with deionized water according to the dissolution instructions.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 1.2 Prepare a sufficient volume of 4X working solution of the test antibody in cell culture medium (four times the test concentration, which should be optimized based on preliminary flow cytometry conditions). For example: 2μg\/ml may be a good starting test concentration for the antibody, so the 4X working solution would be\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e8μg\/ml.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 1.3 Prepare a sufficient volume of 4X UA070122 working solution in cell culture medium. The molar ratio of the test antibody to\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e UA070122 should be 1:2.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 1.4 Mix the 4X test antibody working solution and the 4X UA070122 working solution at a\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e1:1 volume ratio. Incubate at room temperature in the dark for 15min-\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e1h to obtain the Ab- UA070122 \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003ecomplex 2X working solution.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e2. Incubation of Ab- UA070122 Complex with Cells\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 2.1 Cell preparation: Harvest and wash the cells, then adjust the cell concentration using complete medium. For suspension cells, adjust to a concentration of 1-2×10^5\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003ecells\/mL; for adherent cells, adjust to 0.5-1×10^5cells\/mL. Add\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e100μL of cell suspension to each well of a 96-well plate.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e 2.2 Incubation: Add 100μL of the Ab- UA070122 \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003ecomplex\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e2X working solution to each well, and incubate in a 37℃ 5% CO2 incubator.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e3. Flow Cytometry Analysis\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e After\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e \u003c\/span\u003e\u003cfont style=\"font-size:14px;\"\u003e\u003cspan style=\"font-size:10.5pt;\"\u003e18-24h of incubation (adjustable based on the actual internalization time of the antibody), harvest the cells and perform flow cytometry to evaluate antibody internalization. Detection should be performed using the FITC or AF488 channel.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eAvoid vigorous vortex after reconstitution. \u003c\/p\u003e\n\u003cp\u003eAvoid repeated freeze-thaw cycles.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":41519878537291,"sku":"UA070122-10μg","price":735.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":41519878570059,"sku":"UA070122-50μg","price":2200.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41519878602827,"sku":"UA070122-100μg","price":3300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/01909d5f-3d45-4076-8c0b-6df9adc1aa87.jpg?v=1788008615"},{"product_id":"lokivetmab","title":"Lokivetmab","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCanine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCytopoint\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e25-27 kDa (Light Chain) \u0026amp; 53-55 kDa (Heavy Chain) (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePBS, pH7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Matthew Krautmann. Laboratory safety evaluation of lokivetmab, a canine anti-interleukin-31 monoclonal antibody, in dogs.  Vet Immunol Immunopathol. 2023 Apr:258:110574. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eLokivetmab is a caninized anti-IL-31 mAb that binds and neutralizes canine IL-31, resulting in reduced skin lesions and inhibition of pruritus in dogs with AD or allergic dermatitis. Lokivetmab is the first mAb approved for use in dogs with canine AD and allergic dermatitis by regulatory agencies worldwide. Lokivetmab targets canine IL-31 specifically and does not bind to murine or human IL-31 or to any other canine proteins. After s.c. administration, lokivetmab is absorbed and incorporated into the pool of circulating antibodies. The long terminal elimination half-life of lokivetmab in dogs of 16.5 ± 3.0 days is due to recycling via the FcRn receptor and allows for monthly injections to maintain efficacy. Elimination by catabolism is identical to any endogenous protein. Lokivetmab binds at a single epitope on canine IL-31, forming a mAb:IL-31 complex having pharmacokinetics and elimination like unbound lokivetmab.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":41677987217483,"sku":"UA010875-25μg","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41677987250251,"sku":"UA010875-100μg","price":210.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":41677987283019,"sku":"UA010875-500μg","price":380.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b0415ede-090a-4d82-8922-09f276305e05_85cd2331-76a5-424a-8e68-c16361096820.png?v=1787670175"},{"product_id":"ph-sensitive-lgg-labeling-reagents-red-ua070127","title":"pH-sensitive lgG labeling reagents (Red)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e31kDa \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4 with 5% trehalose is added as protectants\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to settie down at the bottom of the tube.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 10uL（10ug package）.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3. After adding sterile water, cover the lid and mix them bygently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAntibody internalization labeling probe. It can bind to candidated antibodies to produce soluble complex that only fluoresce in acidic environments for the study of antibody internalization.\u003cbr\u003eThe pH-sensitive Labeling probe binds to your primary antibodies via Fc binding protein to a pH-dependent fluorescent molecular. This fluorescent complex reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. The internalized antibodies are detected by measuring fluorescence intensity of the cells.\u003cbr\u003e\u003cbr\u003eThis product can becan be used for human IgG1, IgG2, IgG3, IgG4, rabbit IgG, mouse IgG1, IgG2a, IgG2b and IgG3 and can be detected with  Flow cytometry  PE filter.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image image_resized\" style=\"aspect-ratio:1328\/1492;width:51.07%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20084583\/f8828e83-83b8-4824-a425-ae8f43801b63.png?access_token=71e68ad9-8971-4904-adb8-d0fd4fdf6d79\" alt=\"\" width=\"1328\" height=\"1492\"\u003e\u003c\/p\u003e\n\u003cp\u003e1. Incubation of UA070127 with Antibody\u003cbr\u003e 1.1 Reconstitute the lyophilized UA070127 powder with deionized water according to the dissolution instructions.\u003cbr\u003e 1.2 Prepare a sufficient volume of 4X working solution of the test antibody using cell culture medium (four times the test concentration, which should be optimized based on preliminary flow cytometry conditions). For example: 2 μg\/ml may be a good starting test concentration for the antibody, so the 4X working solution would be 8 μg\/ml.\u003cbr\u003e 1.3 Prepare a sufficient volume of 4X UA070127 working solution using cell culture medium. The molar ratio of the test antibody to UA070127 is 1:2.\u003cbr\u003e 1.4 Mix the 4X test antibody working solution and the 4X UA070127 working solution at a 1:1 volume ratio. Incubate at room temperature protected from light for  \u003cfont style=\"color:rgb(73, 80, 87);font-size:14px;\"\u003e15min-\u003c\/font\u003e\u003cspan style=\"color:rgb(73,80,87);\"\u003e\u003cspan style=\"font-size:14px;\"\u003e \u003c\/span\u003e\u003c\/span\u003e 1h to obtain a 2X working solution of the Ab-UA070127 complex.\u003cbr\u003e2. Incubation of Ab-UA070127 Complex with Cells\u003cbr\u003e 2.1 Cell preparation: Harvest and wash the cells, then adjust the cell concentration using complete culture medium. For suspension cells, adjust to a concentration of 1-2×10^5 cells\/mL; for adherent cells, adjust to 0.5-1×10^5 cells\/mL. Add 100 μL of cell suspension to each well of a 96-well plate.\u003cbr\u003e 2.2 Incubation: Add 100 μL of the 2X Ab-UA070127 complex working solution to each well, and culture in a 37°C, 5% CO2 incubator.\u003cbr\u003e3. Flow Cytometry Analysis\u003cbr\u003e After culturing for 18-24h \u003cspan style=\"color:rgb(73,80,87);\"\u003e\u003cspan style=\"font-size:14px;\"\u003e(adjustable based on the actual internalization time of the antibody)\u003c\/span\u003e\u003c\/span\u003e, harvest the cells and perform flow cytometry to evaluate antibody internalization. Detection should be performed using the PE channel.\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":41738018652235,"sku":"UA070127-10μg","price":735.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41738018717771,"sku":"UA070127-100μg","price":3300.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":41738018685003,"sku":"UA070127-50μg","price":2200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/60ad84ae-ea71-4fd2-814b-b753b1a9c19c.jpg?v=1788044413"},{"product_id":"lipopolysaccharide-lps-solution-500x-ua080443","title":"Lipopolysaccharide (LPS) solution (500X)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e500X (2.5mg\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSterile aqueous buffer, no sodium azide\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -20°C.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eLipopolysaccharide (LPS) solution (500X) is a ready-to-use LPS solution derived from E. coli 026:B6. LPS is a major component of the cell wall of Gram-negative bacteria. It is highly immunogenic and can strongly activate immune cells carrying the CD14\/TLR4\/MD2 receptor complex. The reagent is designed for in vitro culture of B cells, monocytes, macrophages, and other cells that are responsive to LPS.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eObserve aseptic practices during use\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100μl","offer_id":41887872286795,"sku":"UA080443-100μl","price":110.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c84d3efe-5248-4b1a-bb4c-39fb8f52d098.png?v=1788508894"},{"product_id":"bodipy-fl-thalidomide-ua080476","title":"BODIPY FL thalidomide","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(51, 21, 21);font-size: 14px\"\u003e732.58\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003ePowder：\u003c\/font\u003e    \u003cfont\u003e-20°C\u003c\/font\u003e\t\u003cfont\u003e3 years\u003c\/font\u003e \u003cfont\u003e；\u003c\/font\u003e \u003cfont\u003e4°C\u003c\/font\u003e\t\u003cfont\u003e2 years\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eIn solvent：\u003c\/font\u003e\t\u003cfont\u003e-80°C\u003c\/font\u003e\t\u003cfont\u003e6 months；-20°C\u003c\/font\u003e\t\u003cfont\u003e1 month\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(51, 21, 21);\"\u003e\u003cfont class=\"text-black\"\u003eBODIPY FL thalidomide is a high-affinity fluorescent probe for human brain proteins, with a \u003c\/font\u003e\u003c\/span\u003e\u003cb style=\"font-weight: 700; color: rgb(51, 21, 21);\"\u003e\u003cfont class=\"text-black\"\u003eKd\u003c\/font\u003e\u003c\/b\u003e\u003cspan style=\"color: rgb(51, 21, 21);\"\u003e\u003cfont class=\"text-black\"\u003e value of 3.6 nM.\u003c\/font\u003e\u003c\/span\u003e\u003cspan style=\"color: rgb(51, 21, 21);\"\u003e\u003cfont class=\"text-black\"\u003eCAS No. : \u003c\/font\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.cn\/cas\/2740620-18-0.html\"\u003e\u003cfont class=\"text-black\"\u003e2740620-18-0\u003c\/font\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont class=\"text-black\"\u003e​\u003c\/font\u003e \u003cfont class=\"text-black\"\u003eIC50 \u0026amp; Target : Cereblon\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont class=\"text-black\"\u003eFormula : C37H43BF2N6O7\u003c\/font\u003e\u003cfont class=\"text-black\"\u003e \u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont class=\"text-black\"\u003eColor: Orange to red\u003c\/font\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":41887872712779,"sku":"UA080476-1mg","price":345.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":41887872745547,"sku":"UA080476-5mg","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_01ea69df-b432-4fe3-86b0-2be911549c25.png?v=1788516124"},{"product_id":"lysotracker-green-dnd-26-ua079025","title":"LysoTracker Green DND-26 \/ Lysosomal Fluorescent Probe (Green)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eRoom temperature transportation. Store at -20℃ in a dry and dark place, avoiding repeated freezing and thawing. Do not store in a refrigerator without a frost. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eLysoTracker Green DND-26 is a cell-permeant, non-fixable green fluorescent dye specifically designed for staining acidic compartments within cells, such as lysosomes. It has an optimal excitation\/emission wavelength of 504\/511 nm and can be effectively excited using a FITC filter. \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e\u003cstrong\u003eSimple, Highly Specific One-Step Staining and Tracking of Acidic Organelles\u003c\/strong\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eThe LysoTracker probe consists of a fluorescent group combined with a weak base, which is only partially protonated under neutral pH conditions. In its neutral charge state, the probe can freely diffuse across the intact plasma membrane of live cells. When the probe diffuses into lysosomes, the weak basic group becomes protonated due to the acidic environment inside the lysosomes. In this charged state, the probe is less likely to diffuse across the organelle membrane, leading to local accumulation within acidic organelles (such as lysosomes) and enabling unique staining. The LysoTracker probe is effective at nanomolar concentrations, highly selective for acidic organelles, and provides a simple one-step staining method without the need for antibody detection.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e\u003cstrong\u003eLysosomes and Their Functions\u003c\/strong\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003eLysosomes are acidic due to the production and storage of digestive enzymes (hydrolases). This acidic environment is capable of degrading carbohydrates, lipids, nucleic acids, and peptides. Extracellular proteins, viruses, or bacteria can be internalized and transported to lysosomes for degradation via the lysosomal proteolytic pathway. Additionally, misfolded proteins or damaged organelles can become targets for lysosomal degradation through the induction of autophagy. Autophagy is crucial for cell differentiation, survival during nutrient deprivation, and normal growth control. Various pH-sensitive probes can take advantage of the inherent acidity of lysosomes, as well as any changes in pH during biosynthesis or disease processes. LysoTracker products can be used for fluorescent detection of acidic environments in live cells and for labeling and tracking acidic organelles such as lysosomes.\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eApplicable equipment: Fluorescence microscope, fluorescence imaging instrument\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"50μl","offer_id":41887874678859,"sku":"UA079025-50μl","price":84.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d7e94502-296b-4f27-b2ce-90759ff91107.png?v=1788519640"},{"product_id":"ph-sensitive-lgg-labeling-reagents-deep-red-ua079026","title":"pH-sensitive lgG labeling reagents (Deep Red)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e31kDa \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4 with 5% trehalose is added as protectants\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample to settie down at the bottom of the tube.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 10uL（10ug package）.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e3. After adding sterile water, cover the lid and mix them bygently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAntibody internalization labeling probe. It can bind to candidated antibodies to produce soluble complex that only fluoresce in acidic environments for the study of antibody internalization.\u003cbr\u003eThe pH-sensitive Labeling probe binds to your primary antibodies via Fc binding protein to a pH-dependent fluorescent molecular. This fluorescent complex reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. The internalized antibodies are detected by measuring fluorescence intensity of the cells.\u003cbr\u003e\u003cbr\u003eThis product can becan be used for human IgG1, IgG2, IgG3, IgG4, rabbit IgG, mouse IgG1, IgG2a, IgG2b and IgG3 and can be detected with  Flow cytometry  Cy5 filter.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1. Preparation of UA079026 and Antibody Incubation\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.1 Reconstitution of UA079026\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Dissolve the lyophilized UA079026 in deionized water according to the specified dissolution method.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.2 Preparation of 4X Antibody Working Solution\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Prepare a sufficient volume of 4X working solution of the test antibody using cell culture medium (four times the test concentration, which is determined based on preliminary flow cytometry conditions). For example, if 2μg\/ml is a good starting test concentration for the antibody, then the 4X working solution would be 8μg\/ml.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.3 Preparation of 4X UA079026 Working Solution\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Prepare a sufficient volume of 4X working solution of UA079026 using cell culture medium. The molar ratio of the test antibody to UA079026 should be 1:2.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e1.4 Mixing and Incubation\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Mix the 4X test antibody working solution and the 4X UA079026 working solution in a 1:1 volume ratio. Incubate at room temperature in the dark for 15min- 1 hour to obtain the 2X Ab-UA079026 complex working solution.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2. Incubation of Ab-UA079026 Complex with Cells\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2.1 Cell Preparation\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Collect and wash the cells. Adjust the cell concentration using complete culture medium. For suspension cells, adjust to a concentration of 1-2×10⁵ cells\/mL. For adherent cells, adjust to a concentration of 0.5-1×10⁵ cells\/mL. Add 100μL of cell suspension to each well of a 96-well plate.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e2.2 Incubation\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- Add 100μL of the 2X Ab-UA079026 complex working solution to each well. Incubate in a 37℃, 5% CO₂ incubator.\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e3. Flow Cytometry Detection\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e- After 18-24 hours of incubation(Adjustable according to the actual endocytosis time of the antibody), collect the cells for flow cytometry to detect the internalization effect of the antibody. Use the Cy5 channel for detection.\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":41887874711627,"sku":"UA079026-10μg","price":735.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":41887874744395,"sku":"UA079026-50μg","price":2200.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":41887874777163,"sku":"UA079026-100μg","price":3300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/58a95213-2f38-40ad-99c1-6d403f10c7d7.png?v=1788084136"},{"product_id":"ph630-deep-red-tfp-ester-amine-reactive-ua079027","title":"pH630 Deep Red TFP Ester, Amine Reactive","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003epH630 Deep Red TFP Ester** is an amine-reactive dye that readily reacts with primary amines on biomolecules to form covalently linked fluorescent pH probes. This dye can be used to label primary amines on proteins, cells, or viruses, creating stable conjugates that fluoresce only in acidic environments. Our low-background pH630 Deep Red dye simplifies the study of internalization processes, yielding more reliable results with less optimization, as it is activated only in late endosomes and lysosomes. With excitation and emission maxima at approximately 640 nm and 655 nm, respectively, pH630 Deep Red can be detected using standard Cy5 filters.\u003cbr\u003e\u003cbr\u003epH630 Deep Red** is a low-background pH sensor dye that exhibits no signal under neutral conditions and fluoresces only in acidic environments. This unique property enables the rapid development and definitive results of assays for antibody internalization, endocytosis, and phagocytic pathways. The dye's approximate pKa value of 5 ensures activation only upon entry into late endosomes and lysosomes, allowing for better distinction between intracellular and extracellular materials. Compatible with Cy5 fluorescence filter sets, pH630 Deep Red has been validated for use in a variety of applications, including flow cytometry, fluorescence microscopy, and high-content screening (HCS).\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100μg","offer_id":41887874842699,"sku":"UA079027-100μg","price":250.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":41887874875467,"sku":"UA079027-1mg","price":1167.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_332067e0-eeb0-4cb9-8277-7329f958f136.png?v=1788519643"},{"product_id":"phab-thiol-reactive-dye-ua079030","title":"pHAb Thiol Reactive Dye","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eThe dyes can be stored at -20°C for up to one month. For long-term storage, they should be kept below -65°C. Avoid repeated freezing and thawing.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cfont\u003epHAb Dyes are pH sensor dyes that have very low fluorescence at pH \u0026gt; 7 and a dramatic increase in fluorescence as the pH of the solution becomes acidic. pHAb Dyes have excitation maxima (Ex) at 532nm and emission maxima (Em) at 560nm.\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003epHAb Reactive Dyes are designed specifically for antibody labeling and can be used for high-throughput antibody internalization screening assays.\u003c\/p\u003e\n\u003cp\u003epHAb Thiol Reactive Dye has a maleimide group that reacts with thiols. This maleimide group is conjugated to the antibody after the cysteine disulfide bonds in the hinge region of the antibody are reduced to thiols through the use of a reducing agent, such as DTT or TCEP. pHAb Reactive Dyes retain their fluorescent response to decreases in pH even after conjugation to antibody.\u003c\/p\u003e\n\u003cp\u003eHere are some recommended instrument parameters: \u003cbr\u003e• Microplate Reader: Tecan Infinite® M1000 Pro (Ex: 532 nm \/ Em: 560 nm) \u003cbr\u003e• Flow Cytometer: Millipore Guava easyCyte 8HT, using yellow fluorescence parameters (Em filter: 583\/26) \u003cbr\u003e• Fluorescence Microscope: Olympus FV 500; TAMRA setting (Ex: 543 nm \/ Em: 565 nm) \u003cbr\u003e• Fluorescence Imaging System: Life Technologies EVOS® FL Cell Imaging System, RFP red cube filter set (Ex: 531 nm \/ Em: 593 nm) \u003cbr\u003e• Fluorescence Gel Scanner: GE Typhoon® 9410; TAMRA setting (Ex: 532 nm \/ Em: 580 nm)\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250μg","offer_id":41887883788363,"sku":"UA079030-250μg","price":284.0,"currency_code":"USD","in_stock":true},{"title":"250μg*4","offer_id":41887883821131,"sku":"UA079030-250μg*4","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_794c7787-7fcc-48c0-a3b0-633089f9eafe.png?v=1788519740"},{"product_id":"phab-amine-reactive-dye-ua079031","title":"pHAb Amine Reactive Dye","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eThe dyes can be stored at -20°C for up to one month. For long-term storage, they should be kept below -65°C. Avoid repeated freezing and thawing.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cfont\u003epHAb Dyes are pH sensor dyes that have very low fluorescence at pH \u0026gt; 7 and a dramatic increase in fluorescence as the pH of the solution becomes acidic. pHAb Dyes have excitation maxima (Ex) at 532nm and emission maxima (Em) at 560nm.\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003epHAb Reactive Dyes are designed specifically for antibody labeling and can be used for high-throughput antibody internalization screening assays.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003epHAb amine-reactive dyes contain an N-hydroxysuccinimide (NHS) ester group, which reacts with primary amine groups present on lysine residues of antibodies.\u003cbr\u003e\u003cbr\u003eA key feature of pHAb dyes is that each dye molecule contains two sulfonate groups. This enhances their solubility in aqueous solutions and reduces aggregation, a common issue observed with non-sulfonated dyes.\u003cbr\u003e\u003cbr\u003eEven after conjugation to antibodies, the pHAb dyes retain their fluorescence responsiveness to changes in pH\u003cbr\u003e\u003cbr\u003eAlthough antibody labeling is their primary application, pHAb dyes can conjugate to any protein containing primary amines on lysine residues or thiol groups on cysteine residues.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003eHere are some recommended instrument parameters: \u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003e• Microplate Reader: Tecan Infinite® M1000 Pro (Ex: 532 nm \/ Em: 560 nm) \u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003e• Flow Cytometer: Millipore Guava easyCyte 8HT, using yellow fluorescence parameters (Em filter: 583\/26) \u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003e• Fluorescence Microscope: Olympus FV 500; TAMRA setting (Ex: 543 nm \/ Em: 565 nm) \u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003e• Fluorescence Imaging System: Life Technologies EVOS® FL Cell Imaging System, RFP red cube filter set (Ex: 531 nm \/ Em: 593 nm) \u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(73, 80, 87);\"\u003e• Fluorescence Gel Scanner: GE Typhoon® 9410; TAMRA setting (Ex: 532 nm \/ Em: 580 nm)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250μg","offer_id":41887883853899,"sku":"UA079031-250μg","price":250.0,"currency_code":"USD","in_stock":true},{"title":"250μg*4","offer_id":41887883886667,"sku":"UA079031-250μg*4","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_0eaf3170-8004-4eab-b9a3-7324a63311df.png?v=1788519742"},{"product_id":"c6-cell-lysate-s0y0003","title":"C6 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42301470703691,"sku":"S0Y0003-100μg","price":135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f88d2624-4d9b-4a4f-a9ae-c8f36dc9861e.png?v=1788523421"},{"product_id":"raw2647-cell-lysate-s0y0012","title":"Raw264.7 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42301470998603,"sku":"S0Y0012-100μg","price":135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_73bcd039-8072-4de6-900f-8ab41f2314bb.png?v=1788523429"},{"product_id":"nih3t3-cell-lysate-s0y0011","title":"NIH\/3T3 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42301471359051,"sku":"S0Y0011-100μg","price":135.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43091408453707,"sku":"S0Y0011-1mg","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a6ba4431-e487-4648-b9ce-edb38cb57643.png?v=1788523433"},{"product_id":"mouse-heart-tissue-lysate-s0y0025","title":"Mouse heart Tissue Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe mouse heart is a fundamental model system for cardiovascular biology, cardiac development, and disease research. This lysate is ideal for studying:Cardiac contractile and structural proteins (e.g., α-actinin, troponins, myosin heavy chain).Cardiac signaling pathways (e.g., adrenergic receptors, Ca²⁺ handling proteins, PKC\/AKT pathways).Heart failure and hypertrophy markers (e.g., ANP, BNP, β-MHC).Cardiac metabolism and mitochondrial function (e.g., PGC-1α, metabolic enzymes).Ischemia\/reperfusion injury and fibrosis-related proteins (e.g., TGF-β, collagen)\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42302101848139,"sku":"S0Y0025-100μg","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_2d375047-d35c-4faa-8e1b-c13f6686f77e.png?v=1788530508"},{"product_id":"mouse-brain-tissue-lysate-s0y0024","title":"Mouse brain Tissue Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe mouse brain is a central model system for neuroscience, neurodegenerative disease, and neurodevelopmental research. This lysate is ideal for studying: Neural signaling pathways (e.g., synaptic proteins, neurotransmitter receptors). Neurodegenerative disease markers (e.g., Aβ, tau, α-synuclein, huntingtin). Neuroinflammation and glial cell biology (e.g., GFAP, IBA1). Epigenetic and metabolic studies in neural tissue\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42302102241355,"sku":"S0Y0024-100μg","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4a1804d7-7800-4137-ad04-98c14165d0e0.png?v=1788530511"},{"product_id":"pc-12-cell-lysate-s0y0021","title":"PC-12 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe PC-12 cell line, derived from rat adrenal pheochromocytoma, is one of the most classic and widely used cell models in neuroscience research. These cells can be induced to differentiate into sympathetic neuron-like cells and extend neurites under the stimulation of nerve growth factor (NGF), making them highly suitable for studying:• The molecular mechanisms of neuronal differentiation and neurite outgrowth.• Neurotransmitter synthesis, storage, and release (particularly catecholamines).• Neuronal signal transduction pathways (e.g., neurotrophic factor signaling, MAPK\/ERK pathways).• Neurodegenerative diseases (such as Parkinson’s disease and Alzheimer’s disease) as cellular models and related protein expression studies.• Neurotoxicity and screening of neuroprotective drugs.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42302102798411,"sku":"S0Y0021-100μg","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_bb22e0e5-589b-46ce-a18d-03427e563c21.png?v=1788530516"},{"product_id":"bcr-flbd-ua086054","title":"Human Single-Cell BCR FL Amplification Kit (BD Platform)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is mainly applied to the determination of paired full-length VDJ sequences in human single B cells. It is compatible with the BD Rhapsody single-cell sequencing platform and uses optimized targeted amplification primer sequences and the most suitable enzyme reaction system. It can simultaneously effectively amplify and detect heavy and light chain sequence information such as IGHG, IGHM, IGHA, IGKC, and IGLC of human single B cells.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style='-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);border-collapse:collapse;border-spacing:0px;box-sizing:border-box;color:rgb(0, 0, 0);font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;height:358px;letter-spacing:normal;margin:0px auto;max-width:100%;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-transform:none;white-space:normal;widows:2;width:588.458px;word-spacing:0px;' border=\"1\"\u003e\u003ctbody style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:257.698px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:165.031px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:165.062px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eTSO\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003e1M MgCl\u003csub style=\"-webkit-tap-highlight-color:transparent;bottom:-0.25em;box-sizing:border-box;font-size:10.5px;line-height:0;position:relative;vertical-align:baseline;\"\u003e2\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e15uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eHybridization Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.6mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Enzyme\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e70uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e160uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Primers\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003edNTP\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e200uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eNuclease Free Water\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003ePCR MasterMix\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.2mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBead RT\/PCR Enhancer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e200uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eVDJ Universal Oligo N1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e65uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eVDJ Universal Oligo N2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBCR N1 Primer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e16uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBCR N2 Primer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e40uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:257.698px;word-break:inherit;\"\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:165.031px;word-break:inherit;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.8mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:0px !important 0px 16px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eRefer to the BD Rhapsody Full-Length VDJ Library Construction Method.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eFile Name: \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eBD Rhapsody™ System Library Preparation Protocol TCR\/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eDocument Number: 23-24017(03)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eDownload Link: \u003c\/span\u003e\u003ca style=\"border-bottom:2px solid rgba(var(--ds-rgba-transparent));border-left:3px solid rgba(var(--ds-rgba-transparent));border-right:3px solid rgba(var(--ds-rgba-transparent));border-top:2px solid rgba(var(--ds-rgba-transparent));color:var(--dsw-alias-brand-text);margin-left:-3px;margin-right:-3px;position:relative;text-decoration:none;transition:box-shadow var(--ds-transition-duration)var(--ds-ease-in-out);\" target=\"_blank\" rel=\"noreferrer\"\u003e\u003cspan\u003ehttps:\/\/scomix.bd.com\/hc\/en-us\/articles\/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px 0px !important;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eNote: In this kit, the amount of Bead RT\/PCR Enhancer is 40 µL, and the corresponding amount of Nuclease-Free Water is 45.2 µL.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4T","offer_id":42302160306251,"sku":"UA086054-4T","price":1667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_ab11a647-bbea-4967-9aed-13624d551e16.png?v=1788523303"},{"product_id":"bcr-flbd-ua086055","title":"Rabbit Single-Cell BCR FL Amplification Kit (BD Platform)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is mainly used for the determination of the paired full-length VDJ sequences in rabbit-derived single B cells. This product is compatible with the BD Rhapsody single-cell sequencing platform. It adopts optimized target amplification primer sequences and the most suitable enzyme reaction system, which can effectively amplify and detect the light and heavy chain sequence information of rabbit-derived single B cells, including IGHA, IGHG, IGHM, IGKC, IGLC, etc.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"background-color:white;border-collapse:collapse;width:441.35pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"588\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;'\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;'\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;'\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eTSO\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan 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style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eHybridization Buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1.6mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eExtension Enzyme\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e70uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eExtension Buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e160uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eExtension Primers\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003edNTP\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e200uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eNuclease Free Water\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003ePCR MasterMix\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1.2mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eBead RT\/PCR Enhancer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e200uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eVDJ Universal Oligo N1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e65uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eVDJ Universal Oligo N2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eBCR N1 Primer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e16uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eBCR N2 Primer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e40uL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:7.5pt;\"\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eElution Buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1.8mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:0px !important 0px 16px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eRefer to the BD Rhapsody full-length VDJ library construction method.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eFile name: \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eBD Rhapsody™ System Library Preparation Protocol TCR\/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)\u003c\/span\u003e\u003c\/i\u003e\u003cbr\u003e\u003cspan\u003eDocument number: 23-24017(03)\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eDownload link: \u003c\/span\u003e\u003ca target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/scomix.bd.com\/hc\/en-us\/articles\/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human\"\u003e\u003c\/a\u003e\u003ca style=\"border-bottom:2px solid rgba(var(--ds-rgba-transparent));border-left:3px solid rgba(var(--ds-rgba-transparent));border-right:3px solid rgba(var(--ds-rgba-transparent));border-top:2px solid rgba(var(--ds-rgba-transparent));color:var(--dsw-alias-brand-text);margin-left:-3px;margin-right:-3px;position:relative;text-decoration:none;transition:box-shadow var(--ds-transition-duration)var(--ds-ease-in-out);\" target=\"_blank\" rel=\"noreferrer\"\u003e\u003cspan\u003ehttps:\/\/scomix.bd.com\/hc\/en-us\/articles\/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px 0px !important;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eNote: In this kit, the volume of Bead RT\/PCR Enhancer is 40 µL, and the corresponding volume of Nuclease-Free Water is 45.2 µL.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4T","offer_id":42302160961611,"sku":"UA086055-4T","price":1667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a91cb4f6-bc34-4efa-8bb3-0559bcc1a308.png?v=1788523306"},{"product_id":"bcr-flbd-ua086056","title":"Mouse Single-Cell BCR FL Amplification Kit (BD Platform)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is mainly used for the determination of the paired full-length VDJ sequences in mouse-derived single B cells. This product is compatible with the BD Rhapsody single-cell sequencing platform. It adopts optimized target amplification primer sequences and the most suitable enzyme reaction system, which can effectively amplify and detect the light and heavy chain sequence information of mouse-derived single B cells, including IGHA, IGHG, IGHM, IGKC, IGLC, etc.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:441.35pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"588\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:30.0pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eTSO\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1M MgCl\u003csub\u003e2\u003c\/sub\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e15uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eHybridization Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1.6mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eExtension Enzyme\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e70uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eExtension Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e160uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eExtension Primers\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003edNTP\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e200uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eNuclease Free Water\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003ePCR MasterMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1.2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eBead RT\/PCR Enhancer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e200uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eVDJ Universal Oligo N1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e65uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eVDJ Universal Oligo N2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e30uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eBCR N1 Primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e16uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eBCR N2 Primer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:16.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e40uL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:7.5pt;\"\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:193.25pt;\" width=\"258\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eElution Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:123.75pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"height:7.5pt;padding:0cm 0cm 0cm 0cm;width:123.8pt;\" width=\"165\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1.8mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);box-sizing:border-box;color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:0px;orphans:2;padding:0px;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eRefer to the BD Rhapsody full-length VDJ library construction method.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);box-sizing:border-box;color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;padding:0px;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eFile name: \u003c\/span\u003e\u003ci style=\"box-sizing:border-box;\"\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eBD Rhapsody™ System Library Preparation Protocol TCR\/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)\u003c\/span\u003e\u003c\/i\u003e\u003cbr\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eDocument number: 23-24017(03)\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eDownload link: \u003c\/span\u003e\u003ca style=\"-webkit-tap-highlight-color:transparent;background-color:transparent;border-color:rgb(15, 17, 21);border-style:none;border-width:0px;box-sizing:border-box;color:rgb(15, 17, 21);margin-left:-3px;margin-right:-3px;position:relative;text-decoration:none;transition:all;\" target=\"_blank\" rel=\"noreferrer\"\u003e\u003cspan style=\"box-sizing:border-box;\"\u003ehttps:\/\/scomix.bd.com\/hc\/en-us\/articles\/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);box-sizing:border-box;color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px 0px !important;orphans:2;padding:0px;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan style=\"box-sizing:border-box;\"\u003eNote: In this kit, the volume of Bead RT\/PCR Enhancer is 40 µL, and the corresponding volume of Nuclease-Free Water is 45.2 µL.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4T","offer_id":42302161649739,"sku":"UA086056-4T","price":1667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4d9d2372-35d3-473c-bedd-2d4765d363f9.png?v=1788523308"},{"product_id":"mbp-6988-ua080518","title":"MBP (69–88)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eYGSLPQKSQRSQDENPVVHF\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(48, 49, 51)\"\u003e2316.51\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20℃\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mg","offer_id":42302164861003,"sku":"UA080518-1mg","price":167.0,"currency_code":"USD","in_stock":true},{"title":"5mg","offer_id":42302164893771,"sku":"UA080518-5mg","price":467.0,"currency_code":"USD","in_stock":true},{"title":"10mg","offer_id":42302164926539,"sku":"UA080518-10mg","price":667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_edb04515-de06-4ac3-bf85-aba49930568c.png?v=1788523315"},{"product_id":"10xc-ua085056","title":"10 x Kinase Assay Buffer C","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eFor long term storage, keep 10X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable \u003cbr\u003eperformance, avoid repeated handling and multiple freeze\/thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eKise Buffer can be used to assay protein kise activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003e250 mM Tris-HCl (pH 7.5), 50 mM beta-glycerophosphate, 20 mM dithiothreitol (DTT), 1 mM \u003c\/span\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003eVO\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e, 100 mM MgCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1. Thaw 10X buffer at 24-30°C, mixing end-over-end.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Dilute the 10X buffer to the desired concentration using ddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eKise Buffer must be supplemented with ATP.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mL","offer_id":42302218960971,"sku":"UA085056-1mL","price":25.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":42302218993739,"sku":"UA085056-5mL","price":85.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":42302219026507,"sku":"UA085056-10mL","price":135.0,"currency_code":"USD","in_stock":true},{"title":"20mL","offer_id":42302219059275,"sku":"UA085056-20mL","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6f8dde87-65c2-4b64-b3d5-1cbfc357bab3.png?v=1788530571"},{"product_id":"5xb-ua085055","title":"5 x Kinase Assay Buffer B","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eFor long term storage, keep 5X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for1-2 weeks. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eKise Buffer can be used to assay protein kise activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003e250 mM Tris, 50 mM CaCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e, 750 mM Cl, 0.25% (w\/v) Brij 35, pH 7.5.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1. Thaw 5X buffer at 24-30°C, mixing end-over-end.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Dilute the 5X buffer to the desired concentration using ddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eFor optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eperformance, avoid repeated handling and multiple freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mL","offer_id":42302219616331,"sku":"UA085055-1mL","price":25.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":42302219649099,"sku":"UA085055-5mL","price":85.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":42302219681867,"sku":"UA085055-10mL","price":135.0,"currency_code":"USD","in_stock":true},{"title":"20mL","offer_id":42302219714635,"sku":"UA085055-20mL","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_38da8689-613e-4378-b131-1c86a1f62019.png?v=1788530573"},{"product_id":"5xa-ua085054","title":"5 x Kinase Assay Buffer A","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eFor long term storage, keep 5X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eKise Buffer can be used to assay protein kise activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003e200 mM Tris-HCl, pH 7.4, 100 mM MgCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e, and 0.5 mg\/mL BSA. Add fresh DTT prior to use to a fil concentration of 250 μM.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1. Thaw 5X buffer at 24-30°C, mixing end-over-end.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Dilute the 5X buffer to the desired concentration using ddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eFor optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eperformance, avoid repeated handling and multiple freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mL","offer_id":42302220206155,"sku":"UA085054-1mL","price":25.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":42302220238923,"sku":"UA085054-5mL","price":85.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":42302220271691,"sku":"UA085054-10mL","price":135.0,"currency_code":"USD","in_stock":true},{"title":"20mL","offer_id":42302220304459,"sku":"UA085054-20mL","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_14446971-9034-4db6-bda2-7ae39e73978b.png?v=1788530576"},{"product_id":"d-ua085057","title":"Kinase Buffer D","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore product at –20°C.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eKise Buffer can be used to assay protein kise activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003e25mM MOPS, pH7.2, 12.5mM β-glycerol-phosphate, 25mM MgCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e, 5mM EGTA and 2mM EDTA. Add to fil concentration of 0.25mM DTT prior to use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eFor optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eperformance, avoid repeated handling and multiple freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mL","offer_id":42302222008395,"sku":"UA085057-1mL","price":25.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":42302222041163,"sku":"UA085057-5mL","price":85.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":42302222073931,"sku":"UA085057-10mL","price":135.0,"currency_code":"USD","in_stock":true},{"title":"20mL","offer_id":42302222106699,"sku":"UA085057-20mL","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f38997ab-9b60-4ff5-9c88-e1b8a23d6c97.png?v=1788530624"},{"product_id":"smad2-knockout-hela-cell-lysate-s0y0023","title":"SMAD2-Knockout HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMutations\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e165bp deletion in exon1 and exon2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue or phenol red.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eSMAD2, also known as MADR2 or JV18-1, is a 58 kDa signaling protein widely expressed in various tissues, including epithelial and mesenchymal cells. It functions as a central intracellular mediator of the transforming growth factor-beta (TGF-β) receptor signaling pathway. Upon TGF-β stimulation, SMAD2 undergoes phosphorylation at serine residues, converting it into its active phosphorylated form. This activated SMAD2 then forms a heteromeric complex with SMAD4, which translocates into the nucleus. Within the nucleus, the complex binds to specific DNA sequences and regulates the transcription of target genes involved in critical biological processes such as cell proliferation, differentiation, apoptosis, and extracellular matrix production. Furthermore, SMAD2 is a key component of the activin receptor signaling pathway and cooperates with other proteins like SMAD3 to precisely modulate cellular responses to external stimuli, thereby maintaining cellular homeostasis. Dysregulation of SMAD2 signaling is closely associated with various pathological conditions, including fibrosis and cancer, where its excessive activation promotes fibrotic tissue deposition and tumor progression. Therefore, a deeper understanding of the regulatory mechanisms of SMAD2 is essential for developing therapeutic strategies targeting these diseases.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1Kit","offer_id":42332729770059,"sku":"S0Y0023-1Kit","price":635.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/243c86fc111846de93da2dc71df3372c.jpg?v=1788170483"},{"product_id":"ramos-cell-lysate-s0y0022","title":"Ramos Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eRamos is a well-established human B-lymphocyte cell line derived from Burkitt's lymphoma. It is a key model for B-cell biology, immunology, and oncology research, widely used in studies of:B-cell receptor (BCR) signaling pathways (e.g., SYK, BTK, PLCγ2). Apoptosis and survival mechanisms (e.g., BCL-2 family proteins, Caspases).B-cell differentiation and activation markers (e.g., CD19, CD20, MHC-II).Lymphoma research and drug screening (e.g., targeted therapy resistance).Epigenetic and gene expression regulation in B-cells.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332729802827,"sku":"S0Y0022-100μg","price":185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a16aea61-0993-4494-8349-ac3f6ece121e.png?v=1788530513"},{"product_id":"k562-cell-lysate-s0y0020","title":"K562 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eK562 is a highly studied human erythroleukemia cell line derived from a patient with chronic myelogenous leukemia in blast crisis. It is a model cell line for hematopoiesis, leukemia research, and immunology, frequently used in studies of: BCR-ABL signaling (Philadelphia chromosome positive). Erythroid and megakaryocytic differentiation. NK cell and cytotoxic T-cell mediated killing assays. Epigenetic and drug resistance studies. This lysate serves as an excellent positive control for Western blotting and other immunoassays.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332729835595,"sku":"S0Y0020-100μg","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6fa54932-932b-4d41-843c-a5573faac38f.png?v=1788530518"},{"product_id":"ikk-gamma-knockout-hela-cell-lysate-s0y0019","title":"IKK gamma-Knockout HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMutations\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1bp (T213) deletion in exon 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue or phenol red.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eIKK\u003c\/span\u003e\u003cspan\u003e-gamma, also known as NEMO or IKBKG, is a key regulatory protein with a molecular weight of approximately 46-50 kDa. Its structure is characterized by coiled-coil domains, enabling it to form a tetramer that functions as a molecular scaffold. The core biological function of Ikk-gamma is to serve as an essential regulatory subunit of the IKK kinase complex; it is responsible for receiving upstream signals and facilitating the assembly and activation of the complex. Within signaling pathways, Ikk-gamma orchestrates the activation of the canonical NF-κB pathway: upon cellular stimulation by agents such as TNFα or LPS, Ikk-gamma recognizes ubiquitination signals, leading to IKK complex activation. This, in turn, triggers the phosphorylation and subsequent degradation of the inhibitory protein IκB, ultimately releasing the NF-κB transcription factor for nuclear translocation. This process initiates the expression of genes involved in immunity, inflammation, and cell survival. Given its pivotal role, mutations in its gene can lead to immune disorders such as NEMO deficiency syndrome, and its dysregulation is intimately linked to cancer and chronic inflammatory diseases.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1Kit","offer_id":42332729868363,"sku":"S0Y0019-1Kit","price":635.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2cace517dc104226b6cb2484c25bec11.jpg?v=1788170495"},{"product_id":"sh-sy5y-cell-lysate-s0y0026","title":"SH-SY5Y Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSH-SY5Y cells are subclones derived from the human neuroblastoma cell line SK-N-SH and represent a classic in vitro model in neuroscience research. They possess the following key characteristics:• Semi-adherent growth with tendency to aggregate, leading to insufficient lysis using conventional lysis buffers.• High expression of neuronal marker proteins including tyrosine hydroxylase (TH), microtubule-associated protein 2 (MAP2), and α‑synuclein.• Sensitivity to neurotoxins, making them widely used in studies of Parkinson’s disease, neuronal differentiation, drug screening, and tumor mechanisms.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332744843339,"sku":"S0Y0026-100μg","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_5eb9e649-dbc0-4e61-9061-94fb26a04530.png?v=1788530536"},{"product_id":"starstreptavidin-polyhrp200-s0d0045","title":"STARStreptavidin-PolyHRP200","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eELISA, CLIA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.2mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at 2-8 °C immediately upon receipt.\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eELISA\u003c\/td\u003e\n\u003ctd\u003e1: 2000-1:8000\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eSTARStreptavidin-PolyHRP\u003cspan style=\"font-size:11.0352pt;color:rgb(0,0,0)\"\u003e conjugates are made of five identical covalent HRP homopolymer blocks that are, also covalently, coupled to multiple streptavidn molecules.  More HRP molecules bring higher sensitivity of the Immunoassays.  STARStreptavidin-PolyHRP\u003cspan style=\"font-size:11.0352pt;color:rgb(0,0,0)\"\u003e \u003c\/span\u003e is easy  to use and user-friendly . The detection processes are same with conventional assays. Take ELISA as an example, the principles of assay scheme is the same (such as number of steps, incubatlon intervals). STARStreptavidin-PolyHRP is versatile for\u003cstrong style=\"font-weight: 600;color: rgb(35, 45, 54);font-size: 15px;background-color: rgb(247, 242, 235)\"\u003e\u003c\/strong\u003e  any of the applicable HRP (colorimetric, fluorimetric)substrate development systems including enhanced chemiluminescence which can be perfect compatible  with existent routine ELISA and CLIA instrumentation.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":42332810313803,"sku":"S0D0045-1ml","price":970.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6e0db970-4c84-4bee-b9af-9498a99396c9_bed44919-6faa-452c-bc73-b9b556eda6b5.png?v=1788109226"},{"product_id":"hepg2-cell-lysate-s0y0007","title":"HepG2 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847571019,"sku":"S0Y0007-100μg","price":300.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43091408224331,"sku":"S0Y0007-1mg","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6980bef0-8acf-42db-ba2b-a38af7650758.png?v=1788523412"},{"product_id":"hela-cell-lysate-s0y0006","title":"HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847603787,"sku":"S0Y0006-100μg","price":135.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43091408257099,"sku":"S0Y0006-1mg","price":416.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e84fc2e3-8dc9-4eea-bf5b-2747ae573868.png?v=1788523415"},{"product_id":"hct116-cell-lysate-s0y0005","title":"HCT116 cell lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847636555,"sku":"S0Y0005-100μg","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_3e676b88-2452-4522-ac2b-78e1591717ff.png?v=1788523416"},{"product_id":"293t-cell-lysate-s0y0002","title":"293T Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847669323,"sku":"S0Y0002-100μg","price":135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_68a8a60c-b404-4bf8-8182-915a80378574.png?v=1788523424"},{"product_id":"mcf-7-cell-lysate-s0y0010","title":"MCF 7 Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847702091,"sku":"S0Y0010-100μg","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_bac1f114-147d-4c1d-8b3d-b73f7950d77c.png?v=1788523435"},{"product_id":"jurkat-cell-lysate-s0y0009","title":"Jurkat cell lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847734859,"sku":"S0Y0009-100μg","price":170.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43091409502283,"sku":"S0Y0009-1mg","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_10f9fc44-f666-423c-a47a-262e949a4f81.png?v=1788523438"},{"product_id":"tmbelisahrp-s0h9993","title":"TMB显色液（ELISA，HRP显色用）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"500mL","offer_id":42949629804619,"sku":"S0H9993-500mL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c08c11905d3e46c1a3eb1de2ce41ab52.jpg?v=1788253207"},{"product_id":"wash-buffertag-magnetic-agarose-s0d0012","title":"Wash Buffer(Tag Magnetic Agarose)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"20ml","offer_id":43031149740107,"sku":"S0D0012-20ml","price":8.33,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":43031149772875,"sku":"S0D0012-50ml","price":14.67,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":43031149805643,"sku":"S0D0012-100ml","price":26.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_0c2df4bf-275c-4cab-9359-d977217a4be9.png?v=1788516061"},{"product_id":"dilution-buffer-tag-agarose-s0d0011","title":"Dilution Buffer (Tag Agarose)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e3 months from date of receipt, 2 to 8 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eThis product is supplied as a 1X working solution for use as a dilution buffer in immunoprecipitation assays with Tag Magnetic Agarose or Tag Agarose Beads.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"20ml","offer_id":43031149903947,"sku":"S0D0011-20ml","price":8.33,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":43031149936715,"sku":"S0D0011-50ml","price":14.67,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":43031149969483,"sku":"S0D0011-100ml","price":26.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_af3a7d05-7898-4844-811f-1291676dd9ad.png?v=1788516060"},{"product_id":"s0d0007","title":"Tissue Lysis Buffer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50ml","offer_id":43089075765323,"sku":"S0D0007-50ml","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_316fa7c2-595b-4bf5-920e-a462892a000b.png?v=1789974064"}],"url":"https:\/\/www.antbioinc.com\/collections\/antibody-elution.oembed?page=6","provider":"AntBio","version":"1.0","type":"link"}