{"title":"abs5","description":"","products":[{"product_id":"human-il-13-elisa-kit","title":"Human IL-13 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 6000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (6000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240123\/5ecb483ff0cd4b7ca728e700208ac65d.png\" alt=\"\" width=\"431\" height=\"251\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240123\/b5326ad2fe63456a8b20d1c60a815389.jpg\" alt=\"\" width=\"304\" height=\"237\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of human IL-13 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated by high-purity recombinant human IL-13 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with high concentrations of human IL-13, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant human IL-13 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IL-13. Interference with human IL-13 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IL-13 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 70px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 29px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 29px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 29px;\"\u003eIL-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12px;\"\u003e\n\u003ctd style=\"width: 48.1381%; height: 12px;\"\u003eIL-4R\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%; height: 12px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u0026lt; td style = \"width: 45.9864%; height:22px; \"\u0026gt; Add the correct reagent exactly according to the instruction steps\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not replaced during sample addition, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eHemolysis, storage for too long, incomplete agglutination, contamination by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-13 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-13 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eALRHMGC116789, BHR1interleukin-13, IL13, IL-13, IL-13 MGC116788, interleukin 13, MGC116786, NC30, P600, human interleukin 13 ELISA kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-13 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-13 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-13 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-13 (interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 inhibits the production of pro-inflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class conversion to IgE, and upregulates CD23\/Fc epsilon RII. IL-13 signaling is transmitted through a receptor complex containing IL-13Rα1 and IL-4Rα1. This complex also functions as a type 2 IL-4 receptor. IL-13 Rα2 binds to IL-13 and prevents IL-13 from signaling through the IL-13 Rα1\/IL-4 Rα complex. IL-13-bound IL-13R2 can directly promote the development of tumor cell invasiveness and tissue fibrosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e93.75pg\/mL-6000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293304856651,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f62152aefbc14ef29936eca0d76e6b88_46416353-5b80-48c5-a931-b6d71c8eeb04.jpg?v=1755257536"},{"product_id":"human-il-15-elisa-kit","title":"Human IL-15 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 1000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (1000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231215\/363544d2df874f5ba5bf9aa6ff808147.png\" alt=\"\" width=\"567\" height=\"321\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231219\/053566c600264a86867ff3858e3dfb0c.jpg\" alt=\"\" width=\"311\" height=\"245\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of human IL-15 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated by high-purity recombinant human IL-15 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with high concentrations of human IL-15, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant human IL-15 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with human IL-15. Interference with human IL-15 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant human IL-15 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 47.5817%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px; text-align: center;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2Rα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003eIL-15R\u003c\/td\u003e\n\u003ctd style=\"width: 48.1332%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u0026lt; tr style = \"height: 22 px; \"\u0026gt;2Low application temperature and short timeIf the temperature is low, extend the incubation time and color development time\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-15 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-15 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 15 detection kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 384px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-15 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-15 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-15 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 32px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 32px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 32px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-15 (Interleukin15) is a cytokine that induces or enhances the differentiation, maintenance or activation of a variety of T cell subsets, including NK, NKT, Th17, Treg, and CD8 + memory cells. It can also induce dendritic cell differentiation and inflammatory activation, exhibit anti-tumor activity, and inhibit lipid deposition in adipocytes. IL-15 interacts with complexes of IL-15Ralpha and IL-2Rbeta on the cell surface and with common γ chains on adjacent cells. This transposition mechanism causes cells to respond to IL-15 even if they do not express IL-15R. Ligation of membrane-associated IL-15\/IL-15Ralpha complexes also induces reverse signaling, which promotes activation of IL-15\/IL-15Ralpha-expressing cells. The activity of free IL-15 is limited by its relationship to soluble IL-15R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293304922187,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0011d97b22f2489284e86b1b129d8420_3536fff6-d15f-48bb-94b6-2a72eb726e54.jpg?v=1755257537"},{"product_id":"human-il-22-elisa-kit","title":"Human IL-22 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSam\u003cspan style=\"color: #000000;\"\u003eple \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e\u003c\/span\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e  1.8 pg \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   31.3 - 2000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e 82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/927fa19e7bf84c67a1176eee1603f24c.jpg\" alt=\"\" width=\"600\" height=\"466\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: IL-22\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIl-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305020491,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d0382f3ac6b044e694c0420f34232043.jpg?v=1755257536"},{"product_id":"human-il-27-elisa-kit","title":"Human IL-27 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human IL-27 monoclonal antibody is coated on the microplate. IL-27 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-27 multi antibody was added to combine with IL-27 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human IL-27 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.8 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e156 - 10000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e82-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/fc4d82c317684056bf12df44a5288b3f.jpg\" alt=\"\" width=\"300\" height=\"236\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIL-27 is composed of ebi3 (associated with the P40 subunit of IL-12 and IL-23) and p28 (associated with the p35 chain of IL-12)Composed of heterodimeric cytokines. IL-27 is expressed by monocytes, endothelial cells, and dendritic cells through a receptor complex consisting of IL-27 R alpha\/wsx-1\/tccr and gp130. IL-27 induces IL-12 receptor expression on naive cd4+ T cells, sensitizing them to subsequent IL-12-induced Th1 development. It also acts as an anti-inflammatory cytokine by limiting the activity of T and NKT cells.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305118795,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/435ba4b405a54c968b342e5b6898e40d.jpg?v=1755257536"},{"product_id":"human-fgf-acidic-elisa-kit","title":"Human FGF Acidic ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human FGF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.19 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e125 - 8000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e96-113%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/bdf12b13825c4695804398cb0c23b9f7.jpg\" alt=\"\" width=\"300\" height=\"232\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFibroblast growth factor acidic (FGF acidic), also known as FGF-1, endothelial cell growth factor (ECGF) and heparin binding growth factor-1 (HBGF-1) are non glycosylated 17-18 kDa polypeptides secreted by a variety of cell types. This molecule is synthesized as an amino acid without obvious signal peptide sequence(AA) protein. Therefore, this ruled out the possibility that it was secreted through the canonical er\/ Golgi pathway. However, it seems to have a unique secretion pathway including heat shock protein, phosphatidylserine, synaptotagmin-1. The result of acidic secretion of FGF is that extracellular disulfide bonds connect homodimers, and its covalent bonds may be destroyed by reducing agents to release bioactive FGF acidity. Unlike FGF-2, acidic FGFs are known to have a 5'alternate start site. The acidic FGF of a 60 AA splice variant has been reported to be a full-length molecule consisting of the first 57 amino acids plus three additional C-terminal amino acids. In experiments, it can bind FGF receptors, but will not activate them. Acidic FGF has a nuclear localization sequence consisting of aa residues 21-27. Acidic FGF exhibits 95% AA properties for mice and rats and 92% AA properties for cattle. Cells known to express acidic FGFs include mammary epithelial cells and neurons (motor and sensory), skeletal muscle and smooth muscle cells, renal proximal tubular cells, endothelial cells, macrophages, keratinocytes, fibroblasts. The FGF family has five receptors (FGF R1-R5). The first four receptors are transmembrane tyrosine kinase receptors, and FGF R5 is a member of IgSF without tyrosine kinase domain. Notably, acidic FGFs have been reported to bind receptors 1 to 4, but not FGF R5, with FGFs. Acidic FGFs can activate tyrosine kinase binding or use the signal pathway of ligand receptor internalization to reach organelles and nuclei in cells. Receptor activation is associated with concurrent FGF heparin interactions. Heparin can bind with acidic FGF to inhibit or activate the receptor. When activated, heparin appears to be a central component connecting the two fgf\/fgf r complexes. This is an approximation of two FGF r molecules to initiate receptor dimerization and signal transduction. Acidic FGFs also bind heparin on the cell surface, with internalization but no cell activation. Acidic FGF is famous for its mitotic activity on endothelial cells. Activation and internalization of membrane receptors are necessary for complete mitosis. Other cells that proliferate in response to acidic FGF include fibrocytes, muscle cells, hepatocytes, mammary epithelium and fibroblasts.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305348171,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f7a0dc91-33dd-4958-9829-7c0cd36f53b1.png?v=1770720291"},{"product_id":"human-fgf-basic-elisa-kit","title":"Human FGF basic ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ebasic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human FGF basic antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the FGF basic present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e\u003cstrong\u003eSample \u003c\/strong\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e\u003c\/span\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e   0.089-0.740 pg \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e   94-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/cf9fd7d18f4943b5b6fe4466e6a5b998.jpg\" alt=\"\" width=\"600\" height=\"466\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: FGF acidic\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFGF basic, also known as FGF-2(fibroblast growth factor-2) or HBGF-2(heparin-binding growth factor-2), is the most intensively studied of the 22 mitogenic proteins in the FGF family.FGF family members share 35-60% amino acids (aa), but only acidic and alkaline FGF lack signal peptides,which are secreted by another pathway. The 18 kDa FGF basic subtype can be found in the cytoplasm and nucleus and is also secretory.It may be present in storage tanks of heparin sulphate proteoglycans (HSPG) inside or on the cell surface.Transcription from alternate initiation sites produces a 21-23 kDA form, found only in the nucleus.\u003c\/p\u003e\n\u003cp\u003eHuman FGF basic sequences at 18 kDa had aa homology of 97% and 99%, respectively, with mouse\/rat and cattle\/sheep FGF basic sequences. However, the destruction of basic FGF genes in mice resulted in relatively mild cardiovascular, skeletal, and neurophenotypes, suggesting that other members of the FGF family compensated for this.Transgenic basal overexpression mainly affects bone development and mineralization.\u003c\/p\u003e\n\u003cp\u003eFour FGF tyrosine kinase receptors (FGF R) and their splicing variants showed differential binding of FGF.FGF basic preferentially binds FGF R1c and 2C with picoluminal affinity.FGF basic also has a number of other binding partners that can fine-tune FGF basic activity depending on their location and number. These include heparin, integrin V 3, soluble FGF R1, FGF binding protein, free gangliosides, platelet-reactive protein, pentammeric protein 3, fibrinogen, 2-macroglobulin, platelet-derived growth factor and platelet factor-4, all of which bind in nanomolar affinity. These molecules can act as co-receptors or adhesion chaperones of the cells, bait or reservoir in the extracellular matrix, while scavengers or chaperones can act as free proteins. FGF basic binding to cell surface HSPG is particularly critical and is necessary for binding, dimerization, and activation of FGF R.\u003c\/p\u003e\n\u003cp\u003eFGF basic regulates normal processes such as angiogenesis, wound healing, tissue repair, learning and memory, and embryonic development and differentiation of the heart, bone, and brain.It is upregitated by inflammatory mediators such as TNF-α, IL-1β, IL-2, PDGF and nitric oxide.Many human tumors express basic FGF, which may be related to tumor angiogenesis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305413707,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5de707cd016445b29ccbbb3a0eddb86b.jpg?v=1755257537"},{"product_id":"human-g-csf-elisa-kit","title":"Human G-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti human G-CSF monoclonal antibody is coated on the microplate. The G-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human G-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plate, standard, anti human G-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e31.3 - 2000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e96-103\u003cspan style=\"font size: 13.3333px;\"\u003e%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/c02ab025b24a485688e2b368c9fd0d77.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation, and activation of hematopoietic cells in the neutrophil lineage. Mature human G-CSF is a 178 amino acid (AA)Of O-glycosylated proteins, containing two intrachain disulfide bonds. In humans, alternative splicing generates a second isoform with a 3-amino acid deletion. Mouse and human G-CSF share 76% amino acid sequence identity, and the two proteins show species cross reactivity. G-CSF is produced by activated monocytes and macrophages, fibroblasts, endothelial cells, astrocytes, neurons, and bone marrow stromal cells. In addition, various tumor cells constitutively express G-CSF.\u003c\/p\u003e\n\u003cp\u003eHuman G-CSF receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to the haematopoietin receptor superfamily. The mature protein consists of a 603 amino acid extracellular domain (ECD), a 23 amino acid transmembrane fragment, and a 186 amino acid cytoplasmic domain. ECD contains an N-terminal Ig like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Alternative splicing of human G-CSF r generates other isoforms, including potentially soluble forms of the receptor. The ECDs of mouse and human G-CSF r share 63% amino acid sequence identity. G-CSF r forms a complex with the ligand in a 2:2 ratio. It is expressed on monocytes, neutrophils, megakaryocytes, platelets, bone marrow progenitor cells, trophoblasts and placentas, endothelial cells, and various tumor cell types.\u003c\/p\u003e\n\u003cp\u003eG-CSF is an important regulator of granulopoiesis in vivo, and mutations in G-CSF R are associated with congenital neutropenia. G-CSF can support the growth of multilineage hematopoietic progenitor cells and mobilize them from the bone marrow into the blood. G-CSF enhances the functional capacity of mature neutrophils and supports their survival by limiting the apoptosis rate. G-CSF also enhanced m-csf-induced monocyte generation caused by hematopoietic progenitor cells and stimulated the proliferation of peripheral Th2 inducing dendritic cells. It promotes the development of T cell immune tolerance and tissue recovery after myocardial infarction and cerebral ischemia. The human G-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure human G-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human G-CSF expressed in E. coli and antibodies against the recombinant protein. Recombinant human G-CSF has been shown to be accurately quantified. The results obtained using natural human G-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of natural human G-CSF.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305446475,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/53129f1fa0a2431eb93b0dba1a41200b.jpg?v=1755257537"},{"product_id":"human-gm-csf-elisa-kit","title":"Human GM-CSF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. The anti human GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePre coated 96 well plate, standard, anti human GM-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.089-0.740  Pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.6 - 1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85-104%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003cimg src=\"http:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20191226\/8aec979acf4a4811bc45e0baf90da1e7.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte macrophage colony stimulating factor (GM-CSF), also known as csf-2, is a common β chain (β c)The polytrophic 30 kDa member of the cytokine family also includes IL-3 and IL-5. GM-CSF adopts \u0026amp;alpha with two intrachain disulfide bonds-Helical configuration. It is secreted by a variety of activated immune, mesenchymal, and epithelial cell types and circulates in the form of variable glycosylated monomers. During inflammation, it is upregulated in a variety of cell types, including encephalitic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF has 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The high affinity receptor of GM-CSF is bound by a 50 kDa ligand α Subunit (gm-csfrα)And 120 kDa signal transduction β C composition. According to reports, the stoichiometry of functional GM-CSF receptor is GM-CSF, gm-csfrα And β The 2:2:2 complex of C. It is worth noting that β The C subunit is shared by the receptor complex of IL-3 and IL-5, and IL-5 may pass through gm-csfrα And β C signaling. GM-CSF can also utilize syndecan-2 as a co receptor (14). GM-CSF has many functions. It induces the production of monocytes, neutrophils, and eosinophils from CD34 + stem cell precursors. It can synergize with IL-4 or Flt-3 ligands to induce the development and maintenance of bone marrow and dermal dendritic cells. It also functions as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. Human GM-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure the levels of human GM-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human GM-CSF expressed in E. coli and antibodies against recombinant factors. Recombinant human GM-CSF has been shown to be accurately quantified. The results obtained using natural human GM-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of human GM-CSF.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305512011,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1ea541b3b7644137bfae58497aac572e.jpg?v=1755257537"},{"product_id":"human-il-4-elisa-kit","title":"Human IL-4 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL of dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 2000pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (2000 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241209\/fecf5a9262c248099d03b1e0b94e202e.png\" alt=\"\" width=\"434\" height=\"250\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241209\/c5ff990e35494e709c6775531d4da833.jpg\" alt=\"\" width=\"428\" height=\"338\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sensitivity: The lowest measurable dose (MDD) of Human IL-4 is generally less than 1.8 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e2. Calibration: This ELISA kit was calibrated with high purity recombinant Human IL-4 protein expressed by E. coli.\u003cbr\u003e\u003cbr\u003e3. Linearity: 6 different samples were spiked with a high concentration of Human IL-4, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant Human IL-4 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-4. Interference with Human IL-4 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant Human IL-4 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003cbr\u003e\u0026lt; td style = \"width: 33.32%; height: 10 px; \"width =\" 33.3200% \"\u0026gt; IL-4Rα\u003ctable style=\"border-collapse: collapse; width: 65.6055%; height: 185px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 31px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 31px;\" width=\"33.3200%\"\u003eOther recombinant proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003eCommon γ Chain\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003eCommon γ Chain\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 14px;\" width=\"33.3200%\"\u003ebovine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eGM-CSF\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003ecanine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003egp130\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003ecotton rat IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-2 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eequine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003efeline IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-3 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eporcine IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 20px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003eIL-4 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003eIL-6\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 20px;\" width=\"33.3200%\"\u003erabbit IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003erat IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-5 Rα\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003erhesus macaque IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-6R\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα1\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-7\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα2\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα1\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003eIL-13Rα2\u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.32%; height: 10px;\" width=\"33.3200%\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003cbr\u003e\u0026lt; tdstyle = \"width: 41.4459%; height: 22px;\" \u0026gt; Substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) contaminated or exposed to metal ions or oxidants\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-4 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-4 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. The reaction was stopped by adding a stop solution, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4, IL-4B_cell stimulatory factor 1, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, pitrakinra\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 362px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-4 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-4 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-4 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 149px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 31px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 31px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 35px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 35px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIL-4 is a secreted protein of 129 amino acids. IL-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils. By acting on multiple types of cells, IL-4 has multiple immune response regulatory functions. IL-4 is involved in several B cell activation processes. It is also a co-stimulator of DNA synthesis. Induction of MHC class II molecule expression on dormant B cells. IL-4 can enhance the secretion and cell surface expression of IgE and IgG1. It also regulates the expression of low-affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes. IL-4 positively regulates IL31RA expression in macrophages.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3 pg\/mL- 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305643083,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4df3fb8d3d94727abfe362cf915ff42_213b1cc1-0954-4844-93ff-2099d3680ca9.jpg?v=1755257536"},{"product_id":"human-porcine-insulin-elisa-kit","title":"Human \/ Porcine Insulin ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses quantitative sandwich enzyme immunoassay technique. A specific anti- Human \/ Porcine Insulin antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the Human \/ Porcine Insulin present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type:\u003c\/strong\u003e sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type: \u003c\/strong\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eS\u003cspan style=\"color: #000000;\"\u003eample \u003c\/span\u003e\u003c\/strong\u003e\u003cspan style=\"color: #000000;\"\u003e\u003ca style=\"color: #000000;\"\u003e\u003cstrong\u003equantity\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e: \u003c\/strong\u003e100ul\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e   2.15 pmol \/ mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e   15.6 - 1000 pmol\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e   85.0-108.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e2-8 ° C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"http:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin_new_bak-english\/20201113\/c36b0514d4ff44bc8a1eaf4cf421cf28.png\" alt=\"\" width=\"345\" height=\"273\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: Insulin\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInsulin and IGF are members of the insulin molecule family. IGF-I and II are structurally homologous to proinsulin and can be thought of as an exaggerated inverted G shape. The G part of the chain is inchain disulfide bonded. Single-stranded proinsulin peptide is composed of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589) and 21 amino acid residues of A chain (aa 90110).The removal of C peptide by proteolysis results in the formation of mature insulin, which is the heterodimers formed by disulfide bonds between A and B chains. Circulating C peptide levels are elevated in hyperinsulinemia, obesity and type 2 diabetes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical hints: \u003c\/strong\u003eFor research use only, not for in vitro diagnosis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305774155,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8e5f1d28-a0e3-4377-8507-a8899eb31a79.png?v=1770714915"},{"product_id":"human-il-2-elisa-kit","title":"Human IL-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Need to bring your own test equipment \u003c\/strong\u003e\u003cbr\u003e1.  Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength) \u003cbr\u003e2.  High precision liquid dispenser and disposable tip \u003cbr\u003e3.  Distilled or deionized water \u003cbr\u003e4.  Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer \u003cbr\u003e5. 500mL Measuring cylinder \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 1. Preparation before the experiment \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Sample collection and storage \u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e② Serum: Use serum separation tubes ( SST ) Collect samples and place samples at room temperature 30 Minutes. Centrifugation 15 Minutes, with a rotation speed of 1000g 。 The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e③ Plasma: Use EDTA , heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000g , and detect it immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing. The sample may need to be used with a diluent ( 1× ) dilution. \u003cbr\u003e\u003cbr\u003e2.  Reagent Preparation ( \u003cstrong\u003e Please place all reagents and samples at room temperature before use and let them stand  \u003c\/strong\u003e\u003cstrong\u003e15 \u003c\/strong\u003e\u003cstrong\u003e Minutes. All experimental samples and standards are recommended \u003c\/strong\u003e\u003cstrong\u003e Do repeat hole detection \u003c\/strong\u003e ） \u003cbr\u003e①1× Preparation of washing solution: The concentrated washing solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 10mL Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared. \u003cbr\u003e②1× Preparation of buffer for dilution: The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid. \u003cstrong\u003e Example: \u003c\/strong\u003e Take 3mL Buffer for concentration and dilution +27mL Distilled water to volume to 30mL 。 In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared. \u003cbr\u003e③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor; Before use, dilute to 1× Working fluid. According to the amount per well 100uL Calculate the required volume. \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 10uL Of 100 Double working concentration of detection antibody, using dilution buffer ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e④SA-HRP ： SA-HRP For 40× Mother liquor, use dilution buffer before use ( 1× ) Diluted and formulated 1× Working fluid, the required amount per hole is 100uL 。 \u003cstrong\u003e Example: \u003c\/strong\u003e Used 10 Hole, then take 25uL Of 40× Mother liquor +975uL Buffer for dilution ( 1× ) Constant volume to 1mL , obtained 1mL Of 1× Detection antibody at working concentration. \u003cbr\u003e⑤ Developer: per well 100uL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light; The developer removed is for the same day use only. \u003cbr\u003e⑥ Standard: Dilution buffer for lyophilized standard ( 1× ) Re-dissolved, redissolved volume 1000uL , obtaining a concentration of 1000pg\/mL Standard mother liquor. Gently shake at least 5 Minutes, it is fully dissolved. Add to each dilution tube 300uL Buffer for dilution ( 1× ）。 Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. Standard mother liquor without dilution can be used as the highest point of the standard curve ( 1000pg\/mL ), buffer for dilution ( 1× ) can be used as a standard curve zero ( 0pg\/mL ）。 \u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240117\/dbd403ac84e44c85925cd9baa5910f7e.png\" alt=\"\" width=\"489\" height=\"277\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e 2. Operation steps \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Prepare all required reagents and standards; \u003cbr\u003e2.  Take out the microplate from the sealed bag that has been equilibrated to room temperature. Please put the unused slats back into the aluminum foil bag and re-seal; \u003cbr\u003e3.  Add to the microplate 300uL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry; \u003cbr\u003e4.  Add different concentration standards, experimental samples or quality control articles into the corresponding wells, and each well 100uL 。 Seal the reaction wells with plate sealing tape and incubate at room temperature 2 hour ;\u003cbr\u003e5.  Suck the liquid in the plate and wash the plate using a washing bottle, a multi-channel plate washer or an automatic plate washer. Washing solution per well 300uL Then the wash liquid in the plate is aspirated off. Repeat Operation 3 Times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper; \u003cbr\u003e6.  Within each well added 100uL Detect antibodies. Seal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour; \u003cbr\u003e7.  Repeat th 5 Step washing operation; \u003cbr\u003e8.  Within each well added 100uLSA-HRP , room temperature incubation 20 Minutes. Be careful to avoid light; \u003cbr\u003e9.  Repeat th 5 Step washing operation; \u003cbr\u003e10.  Within each well added 100uL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light; \u003cbr\u003e11.  Within each well added 50uL Stop solution, the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly; \u003cbr\u003e12.  After addition of stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected; \u003cbr\u003e13.  Calculation results: Add the corrected absorbance values of each standard and sample (OD450-OD540 Or OD570) , average of repeated well readings and then subtract the average zero standard OD Value. Using computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve. Another way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor. \u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240117\/20605d9cc3dd4d47a44c576150ba3234.jpg\" alt=\"\" width=\"357\" height=\"278\"\u003e\u003c\/div\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e The standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 3. Kit parameters \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Recovery: Different levels of human were spiked in cell culture medium samples IL-2 The recovery rate was determined. The recoveries range from 102-104% , the average recovery was in 103% 。 \u003cbr\u003e2.  Sensitivity: Human IL-2 The lowest measurable dose ( MDD ) is generally less than 3.24pg\/mL 。 The lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve. \u003cbr\u003e3.  Correction: This ELISA High purity recombinant human expressed by Escherichia coli IL-2 Corrected by protein. \u003cbr\u003e4.  Linearity: 4 Different samples were spiked with high concentrations of human IL-2 , followed by a diluent ( 1× ) Dilute the sample to the detection range and determine its linearity. \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 61.9296%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003e Dilution factor \u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; text-align: center; height: 22px;\"\u003e Recovery ( % ） \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e106\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 22px;\"\u003e121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 31.4924%; height: 10px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4924%; height: 10px;\"\u003e83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e5.  Specificity: This ELISA Method can detect natural and recombinant human IL-2 Egg whites. The following factors were mixed with diluent ( 1× ) formulated into  50ng\/mL Concentration to detect human IL-2 Cross-reactivity of. Will 50ng\/mL Interference factors incorporated into the intermediate range of recombinant human IL-2 In the reference substance, to detect the effects of human IL-2 Of interference. No significant cross-reactivity or interference was observed. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 62.1904%; height: 132px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e Recombinant human protein \u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e Recombinant mouse protein \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2sRα\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rβ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-2Rγ\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003eIL-4\u003c\/td\u003e\n\u003ctd style=\"width: 48.1381%;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 4. Analysis of frequently asked questions \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1.  Whiteboard (no color appears after color rendering is complete) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Improper storage of kits; Mixing reagents of different kits \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Purchase new kits and pay attention to storage conditions; Not mixable \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Low application temperature and short time \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e If the temperature is low, extend the incubation time and color development time \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Wrong addition or missed addition of reagents \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Add the correct reagent strictly according to the instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e The container used to prepare the solution is not clean or there is a problem with the water \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Use clean containers and qualified distilled water \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e In the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e Follow the instructions strictly \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003e Heterogeneity of reagent temperature \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003e All reagents should be equilibrated at room temperature 30 minute \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003e Detecting antibodies and \/ Or HRP Concentration too low \u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003e Refer to the instructions, do not dilute at will \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cbr\u003e2.  Flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher) \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003e Serial number \u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003e Possible cause \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003e Solutions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Less washing times, insufficient \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Wash as per instructions \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Substrate 3,3',5,5'- Tetramethylbenzidine ( TMB ) contaminated or exposed by metal ions or oxidants \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Use clean containers and qualified distilled water during preparation; Store protected from light \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Incubation temperature is high and \/ Or too long \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Controlling the temperature and time of incubation and final enzymatic reaction \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e The gun tip was not changed during sample loading, resulting in cross-contamination \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Change the gun head for each sample \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Cross contamination of nearby holes \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Vertical clapper, use suitable clapper paper to avoid paper scraps in the hole \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003e Presence of endogenous interfering substances in sample \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003e Estimate possible infectious substances and perform corresponding treatment \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003e Samples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube \u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003e Avoid hemolysis, contamination, long storage and other phenomena \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e 5. Experimental flow chart \u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-2 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ealdesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF, Human interleukin 2 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Please use it within the validity period of the kit (new and old products are shipped randomly) \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-2 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eAfter the unused slats are placed back in the aluminum foil bag with desiccant and sealed, they can be used in 2-8℃ store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-2 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, calculate the dosage and divide it into packages, which can be used in -20°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-2 Detection antibodies\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter the concentrated volume is dissolved, it can be in 2-8°C store 14 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40× The concentration can be at 2-8°C Storage; 1× Working concentration is not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10× Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be found in 2-8°C store 30 Day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20× Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is an α-helix polypeptide with a molecular weight of 15-18kDa with different glycosylations, which belongs to the common γ-chain (γc) cytokine family members. It exists as a monomer with a very short half-life (\u0026lt; 30min). The precursor of human IL-2 has 153 amino acids, including a 20 amino acid signal sequence, and a 133 amino acid mature polypeptide. IL-2 mature protein contains an O-type glycosylation site at threonine position 3 and three cysteines, of which the intra-chain disulfide bond formed by two cysteines is essential for IL-2 activity. The mature human IL-2 amino acid sequence shares 73%, 66%, 78%, and 97% homology to IL-2 in dog, rat, cat, and macaque, respectively. Although human IL-2 has only 60% amino acid homology with highly polymorphic mouse IL-2, human IL-2 is also biologically active on mouse cells. Cells reported to secrete IL-2 include γδ T cells, activated conventional CD4 + and CD8 + T cells, neurons, microglia, hematopoietic stem cells.\u003cbr\u003eThe IL-2 receptor (IL-2R) consists of three subunits, a 55 kDa CD25\/IL-2Rα chain, a 70 kDa CD122\/IL-2Rβ chain, and a 65 kDa CD132\/γc chain. IL-2 first binds to CD25, and the two-subunit complex formed recruits CD122 and CD132, forming a signal complex with four subunits. In addition to forming a complex with IL-2, CD122\/IL-2Rβ can also form a four-subunit signaling complex with IL-15. CD132\/\u003cs\u003e \u003cen\u003e Also acts as a receptor for IL-4, IL-7, IL-9, IL-15 and IL-21 signaling.\u003cbr\u003eIn vitro studies have shown that IL-2 plays an important role in T cell activation and expansion. In vivo, IL-2 is key to the development, maintenance, and function of regulatory T cells (Tregs), and regulatory T cells can provide protection against autoimmune diseases. In addition, IL-2 can also promote autoimmune inflammation in its target organs by regulating the expression of T cell trafficking genes and the production of Th2 cytokines. In CD8 + T cell subsets, IL-2 is a necessary key to obtaining an optimal first-order response and differentiation into terminal effector cells. IL-2 also promotes the development and activation of CD8 + T cells into memory cells.\u003c\/en\u003e\u003c\/s\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 ℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6pg\/mL-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305905227,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/28e08912821d4ab9b9b3c6a9b4306b07_6dcdb292-de0a-4695-bec7-abae030cfcfb.jpg?v=1755257536"},{"product_id":"human-il-6-elisa-kit","title":"Human IL-6 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e)\u003cbr\u003e\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentration and dilution buffer in the kit is 10 × mother liquor, which should be diluted to 1 × working solution with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter using 10 wells, 10 uL of the detection antibody at 100 times the working concentration was taken and volume to 1 mL using diluted buffer (1 ×) to obtain 1 mL of the detection antibody at 1 × working concentration.\u003cbr\u003e\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e\u003cbr\u003e⑤ Developer: According to 100uL per hole, calculate the dosage required for the current test, take out the corresponding volume of developer, and protect it from light; The developer removed is for the same day use only.\u003cbr\u003e\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 600pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (600 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/072d4b0366654b2d907a0b473832bae3.png\" alt=\"\" width=\"432\" height=\"248\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape, and incubate at room temperature for 2 hours;\u003cbr\u003e\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 570nm or 630nm as the correction wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231226\/86a1a6e09102494cb831b1787e3b6b89.jpg\" alt=\"\" width=\"320\" height=\"252\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human IL-6 were spiked into cell culture medium samples and the recovery rate was determined. The recoveries ranged from 93 to 101%, with an average recovery of 98%.\u003cbr\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human IL-6 is generally less than 1.56 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e\u003cbr\u003e3. Linearity: 4 different samples were spiked with a high concentration of Human IL-6, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003ctable style=\"border-collapse: collapse; width: 77.2549%; height: 102px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; text-align: center; height: 22px;\"\u003eRecovery (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 22px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14px;\"\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 31.4869%; height: 14px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e4. Specificity: This ELISA method can detect natural and recombinant Human IL-6 protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human IL-6. Interference with Human IL-6 was detected by incorporating 50 ng\/mL of the interfering factor into the mid-range recombinant Human IL-6 control. No significant cross-reactivity or interference was observed.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 70.4573%; height: 98px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px; width: 24.0666%;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; text-align: center; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003esgp130\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-6 sR\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 22px;\"\u003eIL-2 Rγ\u003c\/td\u003e\u0026lt; td style = \"width: 24.0666%; height: 22px;\" \u0026gt;\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003eIL-6 sR\/sgp130\u003c\/td\u003e\n\u003ctd style=\"width: 24.0666%; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e5. Experimental flow chart\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto; page-break-inside: avoid;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"249\" height=\"503\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-6 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eB cell stimulatory factor-2, B-cell differentiation factor, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFN-beta-2, IL6, IL-6, IL-6B-cell stimulatory factor 2, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A, Human interleukin 6 Elisa kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use it within the validity period of the kit (new and old products are shipped randomly)\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0 auto; page-break-inside: avoid; height: 390px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 57px;\"\u003eHuman IL-6 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 57px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 57px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman IL-6 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003eHuman IL-6 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 33px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 33px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 33px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 44px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 44px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 148px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 37px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 37px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 28px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 28px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 39px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 39px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 39px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInterleukin-6 (IL-6) is a multifunctional cytokine with α-helix structure, 22-28kDa phosphorylation and varying degrees of glycosylation. It plays an important role in the acute phase of disease response, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. Mature human IL-6 has 183 amino acids and 41% homology to mouse and rat IL-6. Alternative splicing within IL-6 produces a variety of isomers, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8 + T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endotheliin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult glial cells, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B cells, and islet beta cells. IL-6 production is usually controlled by glucocorticoids, catecholamines, and secondary sex steroids, and is generally associated with cellular activation. IL-6 in normal human blood is in the range of 1 pg\/mL, slightly increased during menstrual period, severely increased in the middle and late stages of some cancers, and significantly increased after major surgery.\u003cbr\u003eIL-6 elicits cellular signaling through a cell surface receptor, which is a heterodimeric complex consisting of a ligand-binding subunit (IL-6 receptor) and a signal-transferring subunit gp130. Binding of IL-6 to the IL-6 receptor triggers binding of the IL-6 receptor to gp130 and dimerization of gp130. gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptors are produced by alternative splicing and protease cleavage. Through trans-signaling mechanisms, soluble IL-6 and IL-6 receptor complexes can elicit responses in cells that lack IL-6 receptors on their surface but express gp130. The expression of IL-6 receptor is mainly restricted to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Since the gp130 molecule is very widely expressed, trans-signaling enables a wider range of cell types to respond to IL-6. The soluble gp130 spliceosome prevents trans-signaling of IL-6\/IL-6R, but not signaling by other cytokines using the gp130 molecule as a co-receptor.\u003cbr\u003eTogether with tumor necrosis factor α (TNFα) and IL-1, the acute inflammatory response caused by IL-6 plays an almost unique role in fever and acute inflammatory response of the liver. It also plays an important role in the transformation of acute inflammation to acquired immunity or chronic inflammatory diseases. IL-6 dysregulation can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving trans-signaling of IL-6. In the presence of transforming growth factor TGF-β, IL-6 plays an important role in the differentiation of naïve T cells into Th17 inflammatory cells. IL-6 regulates bone resorption and is a major contributor to inflammatory joint damage in rheumatoid arthritis by promoting the activity of Th17 inflammatory cells. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects, such as skeletal muscle secretion of IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes and induces B cells to mature into plasma cells and immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation-related carcinogenesis, such as colitis-related cancers.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent (1 ×) in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e9.38pg\/mL-600pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293305937995,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/5bdc318eaa694e30afae84b8c6a70e2f_53bc666e-c0ee-4698-acfc-5cbdd1620ee9.jpg?v=1755257536"},{"product_id":"human-tnf-α-elisa-kit","title":"Human TNF-α ELISA Kit(Plus)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti human TNF - \u0026amp;alpha  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake and mix well, and then place it at room temperature for 2 hours of incubation process. The tnf-\u0026amp;alpha in the sample  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and TNF - \u0026amp;alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003epre coated 96 well plates, standards, tnf-α One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.42 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e83-113%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20220211\/648dbe2aed8d48f1b0f82c5283f4df7d.png\" alt=\"\" width=\"300\" height=\"269\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTumor necrosis factor alpha; (tnf-α)Also known as cachexia and tnfsf1a, it is an adipokine that participates in systemic inflammation and is also one of the cytokines that stimulate the acute phase response. It is mainly produced by activated macrophages, but also secreted by other types of cells, such as cd4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neurons. It has a variety of regulatory functions in the immune response and can also serve as a potential pyrogen. Tnf-α After responding to stimuli (infectious agents or tissue damage), it activates neutrophils in the systemic circulation, changes the characteristics of vascular endothelial cells, regulates the metabolic activity of other tissues, and exhibits tumor killing activity by inducing local coagulation. Tnf-α It plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information shows that tnf-α It is also involved in the pathogenesis of AIDS. Tnf-α The assay is also very useful for transplantation research.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306003531,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_5da6f482-0e89-4632-bacb-8db6e67d3699.png?v=1770714914"},{"product_id":"human-il-10-elisa-kit","title":"Human IL-10 ELISA Kit(Plus)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific human IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Il-10  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and sample   The concentration of IL-10 was positively correlated. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003ea copy of pre coated 96 well plate, standard, IL-10 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.43 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e3.13-200pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e81-103%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20220225\/bae42deda01b4f29bd290c35921d1fc1.png\" alt=\"\" width=\"300\" height=\"267\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 10 (IL-10)It is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL10 is mainly secreted by monocytes, but less by lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells, and partially activated T cells and B cells. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - \u0026amp; kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306167371,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e846bdac-99ff-4ebb-805f-6ee8c6bfdf47.png?v=1770714914"},{"product_id":"human-ifn-γ-elisa-kit","title":"Human IFN-γ ELISA Kit(Plus)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti human ifn-\u0026amp;gamma  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The ifn-\u0026amp;gamma in the sample  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and ifn-\u0026amp;gamma in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003epre coated 96 well plates, standards, ifn-γ One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.30 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e83-113%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20220211\/7bb30bea730949a1945677e3efab3db8.png\" alt=\"\" width=\"300\" height=\"288\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eγ interferon (ifn-γ)Is a soluble dimeric cytokine and is the only member of type II interferons. It is mainly secreted by natural killer (NK) and natural killer T (NKT) cells and plays a role in innate immunity; It is secreted by CD4 Th1 and CD8 cytotoxic T cells during antigen-specific immunity. Ifn-γ Or type II interferons play an important role in innate and adaptive immunity against virus, some bacteria and protozoan infections. Ifn-γ It is an important activator of macrophages and an inducer of major histocompatibility complex type II (MHC II) expression. Ifn-γ The abnormal expression of Bcl-2 is associated with many autoinflammatory and autoimmune diseases. In addition to directly inhibiting viral replication, ifn-γ Its importance to the immune system is more reflected in its immune stimulation and immune regulation functions.\u003c\/p\u003eIfn-γ in many different pathological conditions, including infection, autoimmune diseases, transplant rejection, allergic reactions, diabetes, etc; It can be used as a disease marker.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306232907,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_ad9dc358-ab46-498f-a2cb-077eb1299991.png?v=1770714914"},{"product_id":"human-vegf-elisa-kit","title":"Human VEGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human VEGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference corrected wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGF, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman VEGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman VEGF Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman VEGF detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor, is an effective regulator of angiogenesis and angiogenesis in fetus and adults. Vascular endothelial growth factor belongs to the PDGF family, a family of proteins characterized by the presence of eight conserved cystine residues at the structure of the cystine knot and the dimer bound by antiparallel disulfide bonds. After alternative cleavage and sequence length of amino acids, humans express different isoforms, including: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189 and VEGF206, among others. Among them, VEGF165 is the highest expression isoform, followed by VEGF121 and VEGF189. With the exception of VEGF121, the other isoforms contain a basic heparin-binding region and do not diffuse freely. Human VEGF165 has 88% homology to the corresponding protein amino acid sequences of mouse and rat. VEGF is expressed in various cells and tissues, including skeletal muscle cells and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34 + stem cells, endothelial cells, fibroblasts, vascular smooth muscle cells, and the like. The expression of VEGF is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatin M and tumor necrosis factor alpha. The expression level of VEGF is also different during development and in adults.\u003cbr\u003eThe dimer of VEGF binds to two related tyrosine kinase receptors, namely VEGFR1 (also called Flt-1) and VEGFR2 (Flk-1\/KDR). VEGF can induce both homodimerization and autophosphorylation of the latter. These receptors have seven extracellular immunoglobulin domains and one intracellular separate tyrosine receptor domain. Both vascular endothelial cells and some other non-endothelial cells express VEGF receptors. Although VEGF has the highest affinity with VEGFR1, VEGFR2 is the main factor regulating angiogenesis of VEGF. VEGF165 also binds to the semaporin receptor, Neuropilin-1, thereby promoting complex formation with VEGFR2.\u003cbr\u003eVEGF is known for its involvement in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, and thus regulates the density and volume of blood vessels. But it doesn't work on the pattern of vascularization. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemokine. Postpartum, VEGF maintains the integrity of vascular endothelial cells and is an effective mitogen for large\/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and the female reproductive cycle. In diseased tissues, VEGF promotes vascular permeability. Therefore, VEGF is involved in the metastatic process of tumors through extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control tumor angiogenesis induced by VEGF. The level of VEGF circulating in the body is related to the extent of autoimmune diseases (such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, etc.).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306265675,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f6bfd547c6e04af9bee254629f4aebbd.jpg?v=1755257537"},{"product_id":"human-egf-elisa-kit","title":"Human EGF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eNeed to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e1. Microplate reader (can measure the absorption value of 450nm detection wavelength and the absorption value of 540nm or 570nm correction wavelength)\u003cbr\u003e2. High-precision liquid dispenser and disposable tip\u003cbr\u003e3. Distilled water or deionized water\u003cbr\u003e4. Bottle washing (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e5. 500mL measuring cylinder\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e1. Sample collection and storage\u003cbr\u003e① Cell culture supernatant: particulate matter should be removed by centrifugation; Test the sample immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator at-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e② Serum: Use a serum separation tube (SST) to collect samples, and place the samples at room temperature for 30 minutes. Centrifuge for 15 minutes at a rotation speed of 1000 g. The serum was removed immediately and tested immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e③ Plasma: Plasma was collected using EDTA, heparin or citric acid as anticoagulant, centrifuged for 15 minutes within 30 minutes after collection, rotated at 1000g, and detected immediately. If the sample is not tested in time after collection, it is recommended to pack it according to the one-time usage amount and store it in a refrigerator ≤-20 ℃ to avoid repeated freezing and thawing. Samples may need to be diluted with diluent (1 ×).\u003cbr\u003e2. Reagent preparation (\u003cstrong\u003ePlease place all reagents and samples at room temperature before use and let them stand\u003c\/strong\u003e\u003cstrong\u003e15\u003c\/strong\u003e\u003cstrong\u003eMinutes. All experimental samples and standards are recommended\u003c\/strong\u003e\u003cstrong\u003eDo repeat hole detection\u003c\/strong\u003e）\u003cbr\u003e① Preparation of 1 × washing liquid: The concentrated washing liquid in the kit is 20 × mother liquid, which needs to be diluted into 1 × working liquid with distilled water before use.\u003cstrong\u003eExample:\u003c\/strong\u003eTake 10mL of concentrated washing solution + 190mL of distilled water and make the volume to 200mL. In actual operation, the amount used can be calculated first, and then prepared.\u003cbr\u003e② Preparation of 1 × dilution buffer: The concentrated dilution buffer in the kit is 10 × mother liquor, and before use, it needs to be diluted to 1 × working liquid with distilled water.\u003cstrong\u003eExample:\u003c\/strong\u003eMake up to 30 mL with 3 mL of concentration and dilution buffer + 27 mL of distilled water. In actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e③ Antibody detection: centrifuge the dry powder to the bottom of the tube, dissolve it with 110uL dilution buffer (1 ×), and let it stand at room temperature for 5 minutes to obtain 100 × mother liquor; Dilute to 1 × working solution before use. Calculate the required volume according to the dosage of 100uL per well.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 10 uL of the detection antibody having a working concentration of 100 times was taken, and the volume was diluted to 1 mL using a dilution buffer (1 ×) to obtain 1 mL of the detection antibody having a working concentration of 1 ×.\u003cbr\u003e④ SA-HRP: SA-HRP is 40 × mother liquor, which needs to be diluted with dilution buffer (1 ×) before use to prepare 1 × working solution, and the required amount per well is 100uL.\u003cstrong\u003eExample:\u003c\/strong\u003eAfter 10 wells were used, 25 uL of 40 × mother liquor + 975 uL of dilution buffer (1 ×) was diluted to 1 mL to obtain 1 mL of detection antibody having a 1 × working concentration.\u003cbr\u003e⑤ Color development solution: According to 100uL per well, calculate the dosage required for the current test, take out the corresponding volume of color development solution, and protect it from light; The chromogenic solution removed is for the same day only.\u003cbr\u003e⑥ Standard: The freeze-dried standard is re-dissolved with dilution buffer (1 ×), and the re-dissolving volume is 1000uL to obtain the standard mother liquor with a concentration of 250pg\/mL. Gently shake for at least 5 minutes and it dissolves well. 300 uL of dilution buffer (1 ×) was added to each dilution tube. Make serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube. The standard mother solution without dilution can be used as the highest point of the standard curve (250 pg\/mL), and the dilution buffer (1 ×) can be used as the zero point of the standard curve (0 pg\/mL).\u003cbr\u003e\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241112\/1e4f67a74dfc43139d3536d892d574fa.png\" alt=\"\" width=\"438\" height=\"260\"\u003e\u003c\/div\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Operation steps\u003c\/strong\u003e\u003cbr\u003e1. Prepare all required reagents and standards;\u003cbr\u003e2. Take out the microplate from the sealed bag that has been balanced to room temperature. Please put the unused slats back into the aluminum foil bag and reseal them;\u003cbr\u003e3. Add 300uL of washing liquid to the microplate, let it stand and soak for 30 seconds, discard the washing liquid and pat the microplate dry on absorbent paper. Please use it immediately and do not let the microplate dry;\u003cbr\u003e4. Add different concentration standards, experimental samples or quality control products to the corresponding wells, 100uL per well. Sealing the reaction hole with plate sealing adhesive paper and incubating at room temperature for 2 hours;\u003cbr\u003e5. Suck off the liquid in the plate and wash the plate with a bottle washer, a multi-channel plate washer or an automatic plate washer. 300 uL of washing solution was added to each well, and then the washing solution in the plate was aspirated off. Repeat the operation 3 times. Trying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results. At the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e6. Add 100 uL of detection antibody to each well. Seal the reaction wells with plate sealing tape and incubate at room temperature for 2 hours;\u003cbr\u003e7. Repeat the plate washing operation in step 5;\u003cbr\u003e8. Add 100uLSA-HRP to each well and incubate at room temperature for 20 minutes. Be careful to avoid light;\u003cbr\u003e9. Repeat the plate washing operation in step 5;\u003cbr\u003e10. Add 100uL of chromogenic solution to each microwell, incubate at room temperature for 5-30 minutes, and avoid light;\u003cbr\u003e11. Add 50uL of stop solution to each microwell, and the color of the solution in the well will change from blue to yellow. If the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e12. Within 30 minutes after adding the stop solution, measure the absorbance value of 450nm using a microplate reader, and set 540nm or 570nm as the calibration wavelength. If dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e13. Calculation Results: Average the corrected absorbance values (OD450-OD540 or OD570), multiple well readings for each standard and sample, and then subtract the average zero standard OD value. Four-parameter logic (4-PL) curve fitting was performed using computer software to create the standard curve. Alternatively, a curve can be generated by plotting the logarithm of the standard concentration versus the logarithm of the corresponding OD value, and the best fit line can be determined by regression analysis. This process can generate a data fit that is sufficiently useful but less accurate. If the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20241112\/5d55d533c80e4a5aa2127bf869fac4cb.jpg\" alt=\"\" width=\"459\" height=\"357\"\u003e\u003c\/div\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003eThe standard curve data provided is for reference only, and the sample content should be calculated according to the standard curve drawn in the same test.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Kit parameters\u003c\/strong\u003e\u003cbr\u003e1. Recovery rate: Different levels of Human EGF were spiked into cell culture medium samples and the recovery rate was determined. The recovery range is 96-103%, and the average recovery is 99%.\u003cem\u003e \u003c\/em\u003e\u003cbr\u003e2. Sensitivity: The lowest measurable dose (MDD) of Human EGF is generally 0.089-0.740 pg\/mL. The lowest measurable value is the corresponding concentration calculated from the mean of the zero-point absorbance values of 20 standard curves plus two standard deviations.\u003cbr\u003e3. Calibration: This ELISA kit was calibrated with high purity recombinant Human EGF protein expressed by sf-21 insect cells.\u003cbr\u003e4. Linearity: 4 different samples were spiked with high concentrations of Human EGF, and then the samples were diluted to the detection range with diluent (1 ×) to determine their linearity.\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 98px; margin: 0px auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eMean\/Expected (%)\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003eRange (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e100-104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e102-108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e103-111\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e107\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center;\"\u003e100-113\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e5. Specificity: This ELISA method can detect natural and recombinant Human EGF protein. The following factors were formulated with diluent (1 ×) at a concentration of 50 ng\/mL to detect cross-reactivity with Human EGF. Interference with Human EGF was detected by incorporating 50 ng\/mL of interfering factor into the mid-range recombinant Human EGF control. No significant cross-reactivity or interference was observed. Cross-reacted approximately 2.3% with recombinant Human Pro-EGF.\u003cbr\u003e\u0026lt; tdstyle = \"width: 24.0691%; text-align: center; height: 22px;\" \u0026gt; EGF R\u003ctable style=\"border-collapse: collapse; width: 73.2725%; height: 54px; margin: 0px auto; break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eRecombinant human protein\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eRecombinant mouse protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\u003ctd style=\"width: 24.0691%; text-align: center; height: 22px;\"\u003eEGF\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 10px;\"\u003eHB-EGF\u003c\/td\u003e\n\u003ctd style=\"width: 24.0691%; text-align: center; height: 10px;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e4. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e1. Whiteboard (after the color development is completed, no color appears)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 98.562%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 22px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 22px;\"\u003eAll reagents should be equilibrated at room temperature for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 7.04137%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4371%; height: 10px;\"\u003eDetection antibody and\/or HRP concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 45.9864%; height: 10px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e2. Flower plate (blank and negative positive controls are normal, but the OD value of specimen wells is obviously higher)\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 97.9085%; height: 164px; margin: 0 auto; page-break-inside: avoid;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; text-align: center; height: 22px;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; text-align: center; height: 22px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; text-align: center; height: 22px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe substrate 3, 3 ', 5, 5'-tetramethylbenzidine (TMB) was contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eHigh incubation temperature and\/or excessive time\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 22px;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 22px;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 22px;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 6.63198%; height: 10px;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 41.4459%; height: 10px;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"width: 46.3869%; height: 10px;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human EGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the EGF present in the sample combines with the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman epidermal growth factor ELISA kit, beta-Urogastrone, EGF, epidermal growth factor (beta-Urogastrone), epidermal growth factor, HOMG4, pro-epidermal growth factor, URG, Urogastrone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman EGF Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman EGF Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman EGF detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe EGF (epidermal growth factor, also known as Urogastrone) precursor is a Group I member of the EGF family of growth factors with a molecular weight of 185kDa. Group I members are molecules that bind and activate the epidermal growth factor receptor (EGFR). EGF family members are synthesized as type I transmembrane (TM) proteins and are proteolyzed to a soluble form. Human epidermal growth factor (EGF) is a fragment in the form of a 1185 amino acid precursor molecule, containing 1010 amino acid extracellular region, 21 amino acid TM region and 153 amino acid cytoplasmic domain. The extracellular domain (ECD) of this precursor has three main structural modules. These include nine BLDLR repeats, one von Willebrand factor A region, and nine EGF-like repeats, and contain the 53 amino acid structure of the mature EGF molecule in the proximal membrane region (amino acids 971 to 1023 of the precursor form). This transmembrane 185kDa isoform (amino acids 21-1023) is found in most body fluids. This process also produces a large number of protein fragments of 40-100kDa, which may be proteolytic degradation products. The production process of 6kDa mature EGF isoforms is unknown. It may originate inside the cell or on the cell surface by hydrolysis of a soluble 160 kDa precursor or a 70 kDa molecular isoform produced after hydrolysis of this precursor by membrane-bound serinase on the cell surface.\u003cbr\u003eNotably, the mature form of EGF, the 185 kDa transmembrane protein, the circulating molecule that has been hydrolyzed but not yet processed, and the 160 kDa precursor molecule are all biologically active. These biological activities are mainly due to the embedding of EGF peptides in these precursor molecules. Modifications of other types of EGF do not have the activity to bind to EGF receptors. There are four possible splice bodies in the expression of EGF protein in the gene encoding EGF, but none of them affect the sequence of mature EGF. Two of these splicers occurred in the extracellular region sequence, deleting amino acids from positions 913 to 953 and amino acids from positions 314 to 355, respectively. The other two splicebodies involve the intracellular region of EGF, replacing amino acids from positions 1125 to 1207 with 12 amino acids and amino acids from positions 1136 to 1207 with 17 amino acids, respectively. The homology of mature human EGF to mouse, rat and pig EGF is 70%, 70% and 85%, respectively. Cells known to express EGF include platelets, cerebral neurons, astrocytes and cerebellar Purkinje cells, Bruner cells (duodenum) and submandibular gland cells, unstained ciliated epithelium, and anterior pituitary cells. EGF has many different physiological effects. At present, the widely recognized function of EGF is mainly based on the binding of EGF receptor-ligand. EGF receptors can also bind to other EGF family proteins, thereby forming heteromultimers and dimerizing with other EGF receptor family members, or associating with other transmembrane proteins such as PDGFR and HGF receptors. In either case, EGF can have an effect on fetal and adult tissues. In the fetus, EGF affects thymocyte growth and differentiation during the double-negative-double-positive stage. During neuronal formation, EGF also appears to stimulate the generation of glial neurons and promote their epithelialization. Finally, it inhibits the maturation of fat cells, thus increasing the preadipocyte count. In adults, EGF can play a role in the milk production process of mammary gland, and can also cause fibroblast mitosis, ECM dissociation and migration, and widely affect various effects related to growth factors.\u003cbr\u003eThe mechanism of activation of EGF receptors, epidermal growth factor receptors (also known as HER1 and ErbB1), is unclear. Current research believes that each EGF molecule and an EGF receptor molecule have two binding sites, which will force the receptor to undergo conformational changes and bind to the second EGF-EGFR complex. This dimer is the EGF receptor with actual function. ErbB2 can also form heterodimers with the EGF receptor, but cannot bind to EGF. This may be because ErbB2 naturally exists as a dimer, which masks its ligand binding site, so that the ligand cannot bind to it. Therefore, it needs to bind an already activated EGF-EGFR complex to form a functional EGF receptor. However, due to the intrinsic electrostatic repulsion, ErbB2 itself cannot form homodimers. EGF can also form heteromultimer with ErbB3: ErbB2 only at high concentrations. The importance of this process is still unknown. Alternatively spliced isoforms of EGFR are present in tumor cells and may be involved in tumorigenesis or sensitize cells to EGF receptor inhibitors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.9pg\/mL-250pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306331211,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/50373dd8d8f64163b53dfe88fcf8ade8.jpg?v=1755257537"},{"product_id":"human-mmp-9-elisa-kit","title":"Human MMP-9 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human MMP-9 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP-9 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ePlease use within the expiration date of the kit\u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95.4116%; margin: 0px auto; break-inside: avoid; height: 190px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; text-align: center; height: 44px;\"\u003eform\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; text-align: center; height: 44px;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; text-align: center; height: 44px;\"\u003eShelf life of diluted or redissolved reagent after unpacking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 36px;\"\u003eHuman MMP-9 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 36px;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 36px;\"\u003eUnused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 13px;\"\u003eHuman MMP-9 Standard\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 13px;\"\u003e2 sticks\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 13px;\"\u003eAfter dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 15px;\"\u003eHuman MMP-9 detection antibody\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 15px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 15px;\"\u003eAfter dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e40 × SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 stick\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 10px;\"\u003e40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 22px;\"\u003e10 × Buffer for concentration and dilution\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 22px;\"\u003e1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 58px;\" rowspan=\"4\"\u003eAfter opening, it can be stored at 2-8 °C for 30 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003eChromogenic liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 44px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 16px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 16px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 10px;\"\u003e20 × concentrated wash buffer\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 10px;\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 23.1659%; height: 14px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 7.04893%; height: 14px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 33.7976%; height: 14px;\"\u003eStored at room temperature, to avoid contamination, not reusable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMatrix metalloproteinases (MMPs), also known as matrixins, are members of the zinc-calcium-dependent proteolytic enzyme family, which can break down the extracellular matrix (ECM) and participate in the processing of various molecules in different subcellular environments. MMPs play important functions in numerous physiological processes, such as embryonic development, morphogenesis, reproduction, and tissue remodeling. Also involved in inflammation as well as autoimmune diseases such as arthritis, cancer and cardiovascular diseases. The amount of newly synthesized MMPs is mainly regulated at the transcriptional level, and the proteolytic activity of already existing MMPs is controlled not only by the activity of the zymogen, but also by the inhibition of enzyme activity by endogenous inhibitors, such as α2-macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs).\u003cbr\u003eMMP-9 (also known as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, type V collagenase) is secreted as a proglycosylation enzyme. Activation of the zymogen involves proteolytic removal of the N-terminal precursor region, resulting in the formation of an active enzyme of 82 kDa. Active human MMP-9 has 72% and 74% amino acid sequence homology with mouse and rat MMP-9, respectively. In addition to the zinc binding site, the catalytic domain contains three consecutive fibronectin type II homologous units that bind to gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal heme-like domain. In vitro experiments, after treatment with 4-aminophenyl ethyl acetate (APMA), the enzymogen can not only produce active enzyme, but also produce a C-terminal truncated form with equivalent activity. MMP-9 can degrade components in ECM, especially with very high specificity for denatured collagen (gelatin). MMP-9 also cleaves collagen types III, IV, V, XI, as well as elastin, nestin-1, and vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g., IL-1β, CXCL8\/IL-8, CXCL7, CXCL4, CXCL1, LatentTGF-β, membrane-bound TNF-α, VEGF, and FGFbasic), beta-amyloid, substance P, myelin basic protein. This action may increase or decrease the biological activity of these soluble factors, and may also cause them to be released from association with the ECM. MMP-9 can also trigger signaling pathways through various membrane proteins or induce them to fall off the cell membrane to inhibit signaling pathways (E.g. CD44, E-cadherin, integrin, ICAM-1 and IL-2Rα).\u003cbr\u003eMMP-9 is produced by a variety of normal and transformed cells, including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial cells, and epithelial cells. It affects physiological and pathological angiogenesis and vascular remodeling. Activated neutrophils release the MMP-9 precursor and do not bind to TIMP-1, allowing pro-angiogenic FGF-2 to be released from the ECM. The complex of MMP-9 and TIMP-1 failed to induce the release of FGF-2. Neutrophil-derived MMP-9 can exacerbate the inflammatory response, inducing the release of additional neutrophil MMP-9 by the production of collagen-derived polypeptides. MMP-9 also plays an important role in bone formation and remodeling, methamphetamine-induced behavioral sensitization and response, regulation of neuronal synaptic remodeling, trophoblast invasion during implantation, and activation of serine protease inhibitor α1. MMP-9-mediated shedding of adhesion molecules also has a direct effect on tumor cell invasion.\u003cbr\u003eCirculating levels of MMP-9 are elevated in many inflammatory disorders, including the formation of intraluminal thrombus, atherosclerosis, Crohn's disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy. The ratio of MMP-9 to TIMP-1 was elevated in multiple sclerosis sera and cystic fibrous sputum but decreased in cytomegalovirus-infected sera. The level of free MMP-9 and the level of MMP-9 and lipocalin-2\/NGAL complex were elevated in the urine of patients with ovarian cancer and patients with uterine urethral tract infection, respectively.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Please use the kit within the validity period.\u003cbr\u003e2. The components of different kits and different batch kits cannot be mixed.\u003cbr\u003e3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.\u003cbr\u003e4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e6. For scientific research only, not for in vitro diagnosis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKit unopened, stored at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31.3pg\/mL-2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306363979,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d30d0a4d685b4674a16036febbc8f9df.jpg?v=1755257537"},{"product_id":"human-pd-1-elisa-kit","title":"Human PD-1 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eprotein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePD-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. You need to bring your own test equipment\u003c\/strong\u003e\u003cbr\u003e\n1. Microplate reader (measurable 450nm Absorption value of detection wavelength and 540nm Or 570nm Absorption value of corrected wavelength)\u003cbr\u003e\n2. High precision liquid dispenser and disposable tip\u003cbr\u003e\n3. Distilled or deionized water\u003cbr\u003e\n4. Bottle washer (spray bottle), multi-channel plate washer or automatic plate washer\u003cbr\u003e\n5. 500mL Measuring cylinder\u003cbr\u003e\u003cbr\u003e\n\n\u003cstrong\u003e2. Preparation before the experiment\u003c\/strong\u003e\u003cbr\u003e\n\u003cstrong\u003e1 Sample collection and storage\u003c\/strong\u003e\u003cbr\u003e\n\u003cstrong\u003eCell culture supernatant\u003c\/strong\u003e: Particulate matter should be removed by centrifugation; Test the sample immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in -20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) Dilution.\u003cbr\u003e\n\u003cstrong\u003eSerum:\u003c\/strong\u003e Using serum separation tubes (SST) Collect samples and place samples at room temperature 30 Minutes.\u003cbr\u003e\nCentrifugation 15 Minutes, with a rotation speed of 1000 g。\u003cbr\u003e\nThe serum was removed immediately and tested immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) dilution.\u003cbr\u003e\n\u003cstrong\u003ePlasma:\u003c\/strong\u003e Using EDTA, heparin or citric acid as an anticoagulant to collect plasma, after collection 30 Centrifuge within minutes 15 Minutes, with a rotation speed of 1000 g, and detect it immediately.\u003cbr\u003e\nIf the sample is not tested in time after collection, it is recommended to pack it according to the amount used once and store it frozen in ≤-20℃ In the refrigerator, avoid repeated freezing and thawing.\u003cbr\u003e\nThe sample may need to be used with a diluent (1×) dilution.\u003cbr\u003e\n\u003cstrong\u003e2 Preparation work before testing\u003c\/strong\u003e\u003cbr\u003e\nPlease place all reagents and samples at room temperature before use and let them stand 15 Minutes.\u003cbr\u003e\nIt is recommended that all experimental samples and standards be repeatedly tested\u003cbr\u003e\n\u003cstrong\u003e1× Wash solution preparation:\u003c\/strong\u003e The concentrated wash solution in the kit is 20× Mother liquor should be diluted with distilled water before use to 1× Working fluid.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Take 10ml Concentrated wash +190mL Distilled water to volume to 200mL In actual operation, the usage amount can be calculated first, and then prepared.\u003cbr\u003e\n\u003cstrong\u003e1× Buffer preparation for dilution:\u003c\/strong\u003e The concentration and dilution buffer in the kit is 10× Mother liquor, dilute with distilled water before use to 1× Working fluid.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Take 3ml Buffer for concentration and dilution +27mL Distilled water to volume to 30mL。\u003cbr\u003e\nIn actual operation, the required amount of dilution buffer solution can be calculated according to the sample dilution factor, and then prepared.\u003cbr\u003e\n\u003cstrong\u003eAntibody detected:\u003c\/strong\u003e Centrifuge the dry powder to the bottom of the tube and use 110μL Buffer for dilution (1×) Dissolve and let stand at room temperature 5min Later obtained 100× Mother liquor;\u003cbr\u003e\nBefore use, dilute to 1× Working fluid.\u003cbr\u003e\nAccording to the amount per well 100μL Calculate the required volume.\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Used 10 Hole, then take 10μL Of 100 Double working concentration of detection antibody, using dilution buffer (1×) Constant volume to 1mL, obtained 1ml Of 1× Detection antibody at working concentration\u003cbr\u003e\n\u003cstrong\u003eSA-HRP：\u003c\/strong\u003e SA-HRP For 40× Mother liquor, use dilution buffer before use (1×) Diluted and formulated 1× Working fluid, the required amount per hole is 100 μL。\u003cbr\u003e\n\u003cstrong\u003eExample:\u003c\/strong\u003e Used 10 Hole, then take 25μL Of 40× Mother liquor +975uL Buffer for dilution (1×) Constant volume to 1mL, obtained 1ml Of 1× Detection antibody at working concentration.\u003cbr\u003e\n\u003cstrong\u003eColor development solution:\u003c\/strong\u003e Per hole 100 μL Calculate the dosage required for the current test, take out the corresponding volume of color developer, and protect it from light;\u003cbr\u003e\nThe developer removed is for the same day use only.\u003cbr\u003e\n\u003cstrong\u003eStandard:\u003c\/strong\u003e Dilution buffer for lyophilized standards (1×) re-dissolve,\u003cbr\u003e\n\u003cstrong\u003eRe-dissolve Volume 1000μL\u003c\/strong\u003e, obtaining a concentration of 10000pg\/mL Standard mother liquor.\u003cbr\u003e\nGently shake at least 5 Minutes, it is fully dissolved.\u003cbr\u003e\nAdd to each dilution tube 300 μL \u003cstrong\u003eDilution Buffer （1×）\u003c\/strong\u003e。\u003cbr\u003e\nMake serial dilutions of the standard mother liquor according to the figure below, and each tube must be thoroughly mixed before pipetting to the next tube.\u003cbr\u003e\nStandard mother liquor without dilution can be used as the highest point of standard curve (10000pg\/mL)，\u003cbr\u003e\n\u003cstrong\u003eDilution Buffer （1×）\u003c\/strong\u003e Can be used as a standard curve zero (0 pg\/mL）。\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20251224\/a8366755e6274cd0ac4efc73842d4a48.png\" alt=\"\" width=\"354\" height=\"202\"\u003e\u003cbr\u003e\n\u003cstrong\u003e3. Experimental operation steps\u003c\/strong\u003e\u003cbr\u003e\n1 Prepare all required reagents and standards;\u003cbr\u003e\n2 Take out the microplate from the sealed bag that has been balanced to room temperature.\u003cbr\u003e\nPlease put the unused slats back into the aluminum foil bag and re-seal;\u003cbr\u003e\n3 Adding to the microplate 300μL Washing liquid, let stand and soak 30 Seconds, discard the lotion and pat the microplate dry on absorbent paper.\u003cbr\u003e\nPlease use it immediately and do not let the microplate dry;\u003cbr\u003e\n4 Add different concentration standard substances, experimental samples or quality control substances into corresponding wells, and each well 100μL。\u003cbr\u003e\nSeal the reaction wells with plate sealing tape and incubate at room temperature 2 hour;\u003cbr\u003e\n5 Suck off the liquid in the plate, and use a bottle washing machine, a multi-channel plate washer or an automatic plate washer to wash the plate.\u003cbr\u003e\nWashing solution per well 300μL Then the wash liquid in the plate is aspirated off.\u003cbr\u003e\nRepeat Operation 3 Times.\u003cbr\u003e\nTrying to absorb the residual liquid as much as possible every time you wash the plate will help to get good experimental results.\u003cbr\u003e\nAt the end of the last plate washing, please suck all the liquid in the plate or turn the plate upside down, and pat all the residual liquid dry on absorbent paper;\u003cbr\u003e\n6 Adding in each microwell 100μL Detect antibodies.\u003cbr\u003e\nSeal the reaction wells with plate sealing tape and incubate at room temperature 2 Hour;\u003cbr\u003e\n7 Repeat the first 5 Step washing operation;\u003cbr\u003e\n8 Adding in each microwell 100μL SA-HRP, room temperature incubation 20 Minutes.\u003cbr\u003e\nBe careful to avoid light;\u003cbr\u003e\n9 Repeat the first 5 Step washing operation;\u003cbr\u003e\n10 Adding in each microwell 100 μL Chromogenic solution, incubate at room temperature 5-30 Minutes, pay attention to avoiding light;\u003cbr\u003e\n11 Adding in each microwell 50μL Stop solution, the color of the solution in the well will change from blue to yellow.\u003cbr\u003e\nIf the color of the solution changes to green or the color changes are inconsistent, pat the microplate gently to mix the solution evenly;\u003cbr\u003e\n12 After adding the stop solution 30 Within minutes, measured using a plate reader 450nm Absorbance value, set 540nm Or 570nm As a correction wavelength.\u003cbr\u003e\nIf dual-wavelength correction is not used, the accuracy of the results may be affected;\u003cbr\u003e\n13 Calculation results: add the corrected absorbance values of each standard and sample (OD450-OD540\/OD570), average of repeated well readings and then subtract the average zero standard OD Value.\u003cbr\u003e\nUsing computer software for four-parameter logic (4-PL) Curve fitting creates a standard curve.\u003cbr\u003e\nAnother way is to plot the standard concentration and make the logarithm with the corresponding OD The values were logarithmic to generate a curve, and the best fit line was determined by regression analysis.\u003cbr\u003e\nThis process can generate a data fit that is sufficiently useful but less accurate.\u003cbr\u003e\nIf the sample is diluted, the concentration should be calculated by multiplying the dilution factor.\u003cbr\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20251224\/ab9ea81638f747c8ade624dc1ec313f3.png\" alt=\"\" width=\"263\" height=\"184\"\u003e\u003cbr\u003e\n\u003cstrong\u003e4. Kit parameters\u003c\/strong\u003e\u003cbr\u003e\n1 Recovery rate\u003cbr\u003e\nSpiked different levels of human in cell culture medium samples PD-1 The recovery rate was determined.\u003cbr\u003e\nThe recoveries range from 85.0-111.9%, the average recovery was in 100.9%。\u003cbr\u003e\nInspired human serum samples with different levels of human PD-1 The recovery rate was determined.\u003cbr\u003e\nThe recoveries range from 99.3-121.1%, the average recovery was in 111.0%。\u003cbr\u003e\n2 Sensitivity\u003cbr\u003e\nPerson PD-1 The lowest measurable dose (MDD） Generally less than 18.9 pg\/mL。\u003cbr\u003e\nThe lowest measurable value is determined according to 20 The corresponding concentration is calculated by adding two standard deviations to the mean value of the zero-point absorbance values of each standard curve.\u003cbr\u003e\n3 Correction\u003cbr\u003e\nThis ELISA The kit NS0 Expressed high purity recombinant human PD-1 Corrected by protein.\u003cbr\u003e\n4 Linearity\u003cbr\u003e\n4 Different samples were spiked with high concentrations of human PD-1， Then using a diluent (1×） Dilute the sample to the detection range, Its linearity was determined.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.1035%; margin-left: auto; margin-right: auto; height: 168px;\" border=\"1\" width=\"99%\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003eDilution factor\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003eAverage \/ Expected value (%）\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003escope (%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:2\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e101.1\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e89.9 – 111.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:4\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e106.0\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e93.1 – 115.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:8\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e102.4\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e89.0 – 118.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23%;\" width=\"23%\"\u003e1:16\u003c\/td\u003e\n\u003ctd style=\"width: 32.7406%;\" width=\"47%\"\u003e104.3\u003c\/td\u003e\n\u003ctd style=\"width: 31.4839%;\" width=\"29%\"\u003e88.3 – 118.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n5 Specificity,\u003cbr\u003e\nThis ELISA Method can detect natural and recombinant human PD-1 Egg whites.\u003cbr\u003e\nThe following factors were mixed with diluent (1×） Formulated into 100 ng\/mL Concentration to detect human PD-1 Cross-reactivity of.\u003cbr\u003e\nWill 100 ng\/mL Interference factors incorporated into the intermediate range of recombinant human PD-1 In the reference substance, To detect human PD-1 Of interference.\u003cbr\u003e\nNo significant cross-reactivity or interference was observed.\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 82.2387%; height: 180px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eRecombinant porcine protein\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eB7-H1\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eCD28\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eCTLA-4\/Fc Chimera\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"width: 100%;\"\u003eICOS\/ Fc Chimer\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003cstrong\u003e5. Analysis of frequently asked questions\u003c\/strong\u003e\u003cbr\u003e\n1 Whiteboard (after color rendering is completed, no color appears)\u003cbr\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.1359%; height: 286px; margin-left: auto; margin-right: auto;\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eImproper storage of kits; Mixing reagents of different kits\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003ePurchase new kits and pay attention to storage conditions; Not mixable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eLow application temperature and short time\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eIf the temperature is low, extend the incubation time and color development time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eWrong addition or missed addition of reagents\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eAdd the correct reagent strictly according to the instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eThe container used to prepare the solution is not clean or there is a problem with the water\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eUse clean containers and qualified distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eIn the process of plate washing, the soaking time is long, the times of plate washing are too many, and the impact force of plate washing is large\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eFollow the instructions strictly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eHeterogeneity of reagent temperature\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eAll reagents should be equilibrated at room temperature 30 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"width: 8.91625%; height: 16px; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 44.0676%; height: 16px;\"\u003eDetecting antibodies and \/ Or HRP Concentration too low\u003c\/td\u003e\n\u003ctd style=\"width: 75.9324%; height: 16px;\"\u003eRefer to the instructions, do not dilute at will\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n2, flower plate (blank, negative positive control normal, but specimen well OD Values are significantly higher)\u003cbr\u003e\n\u003ctable style=\"break-inside: avoid; border-collapse: collapse; margin-left: auto; margin-right: auto; height: 164px; width: 80.4555%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003eSerial number\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003ePossible cause\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eSolutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eLess washing times, insufficient\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eWash as per instructions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eSubstrate 3,3',5,5'- Tetramethylbenzidine (TMB) contaminated or exposed by metal ions or oxidants\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eUse clean containers and qualified distilled water during preparation; Store protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eIncubation temperature is high and \/ Or too long\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eControlling the temperature and time of incubation and final enzymatic reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eThe gun tip was not changed during sample loading, resulting in cross-contamination\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eChange the gun head for each sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eCross contamination of nearby holes\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eVertical clapper, use suitable clapper paper to avoid paper scraps in the hole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003ePresence of endogenous interfering substances in sample\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eEstimate possible infectious substances and perform corresponding treatment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16px;\"\u003e\n\u003ctd style=\"height: 16px; width: 8.50679%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 44.5518%;\"\u003eSamples were hemolyzed, stored for too long, incomplete agglutination, contaminated by bacteria, influence of additives in blood collection tube\u003c\/td\u003e\n\u003ctd style=\"height: 16px; width: 75.4482%;\"\u003eAvoid hemolysis, contamination, long storage and other phenomena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003cstrong\u003e5. Experimental flow chart\u003c\/strong\u003e\u003cbr\u003e\n\u003cimg style=\"page-break-inside: avoid; display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/3e54acc7e71f47368f1991b828798461.png\" alt=\"\" width=\"221\" height=\"447\"\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human PD-1 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the wells of the enzyme label plate. After incubation, PD-1 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 90.8654%; margin-left: auto; margin-right: auto; height: 345px;\" border=\"1\" width=\"576\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e\u003cstrong\u003e form \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e\u003cstrong\u003e Shelf life of diluted or redissolved reagent after unpacking \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1 Microplate\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Block \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e The unused slats are placed back in the aluminum foil bag with desiccant and sealed, and placed in 2-8℃ Storage, 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1  Standard \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e2 branch \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e After dissolution, calculate the dosage and divide it into packages, which can be used in -20℃ store 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003eHuman PD-1  Detection antibodies \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e After the concentrated volume is dissolved, it can be in 2-8℃ storage 14 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e40×SA-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 branch \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e40 Double concentration at 2-8℃ Preservation; 1 Double working concentration is not recommended for storage \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e10× Buffer for concentration and dilution \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" rowspan=\"4\" width=\"144\"\u003e     After opening, 2-8℃ Storage, 30 Day \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Chromogenic liquid \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Stop liquid \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e20× Concentrated Wash Buffer \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e1 Bottle \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 29.0655%;\" width=\"144\"\u003e Sealing film \u003c\/td\u003e\n\u003ctd style=\"width: 11.3512%;\" width=\"110\"\u003e3 Zhang \u003c\/td\u003e\n\u003ctd style=\"width: 56.2277%;\" width=\"144\"\u003e Store at room temperature, to avoid contamination, cannot be reused \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eProgrammed death-1 receptor (PD-1), also known as CD279, is a type I transmembrane protein of the CD28 family of immunomodulatory receptors, other members of which include CD28, CTLA-4, ICOS, and BTLA. Its cytoplasmic tail contains two tyrosine residues that make up the tyrosine-based immunoreceptor inhibitory domain (ITIM) and switch domain (ITSM), which are important for mediating PD-1 signal transduction. As a monomeric receptor, PD-1 can interact with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) in a ratio of 1: 1. PD-1 is expressed in activated T cells, B cells, monocytes and dendritic cells, while PD-L1 is expressed in non-hematopoietic cells (such as lung endothelial cells and hepatocytes) in addition to the above cells. The combination of PD-L1 and PD-1 can induce co-inhibitory signals, promoting T cell inactivation, apoptosis, and functional failure. Therefore, PD-1 and PD-L1 interaction is a key regulator of immune response threshold and peripheral immune tolerance. Blocking the interaction of PD-1 and PD-L1 through antibody or gene regulation can accelerate tumor eradication, suggesting the potential of PD-1 as a target for cancer immunotherapy.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Only for scientific research, not for in vitro diagnosis;\u003cbr\u003e2. Please use it within the validity period of the kit;\u003cbr\u003e3. The components of different kits and kits with different batch numbers cannot be mixed;\u003cbr\u003e4. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except for the last step of dilution with diluent;\u003cbr\u003e5. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.\u003cbr\u003e6. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.\u003cbr\u003e\u003cbr\u003e\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe unopened kit is stored at 2-8 ℃ and has a validity period of one year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306560587,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d4728ef19501494fb3918af11979c5e8.jpg?v=1755257537"},{"product_id":"human-il-2-high-sensitivity-elisa-kit","title":"Human IL-2 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-2 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-2 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Somponents: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-2 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.31pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e3.19-250 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e101-129%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/03f8087555db4d43bd7dd5b5d07c69e9.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 2 (IL-2) is a 15 kDa glycoprotein. In humans, it is encoded by a single gene located in the q26 – 28 region of chromosome 4. It is a class of signaling molecules in the immune system, regulating the immune function of leukocytes(white blood cells, usually lymphocytes) activity. IL-2 is the body's natural response to microbial infection, distinguishing foreign and self antigens. IL-2 functions by interacting with the IL-2 receptor, which is composed of three chains, namely α Chain (CD25), β Chain (CD122) and γ Chain (CD132). Monitoring the content of IL-2 in serum provides a more detailed perspective for a variety of pathological conditions, including cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306855499,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_0bd4c7fa-8fcc-4b23-9e76-f7af399d038c.png?v=1770714912"},{"product_id":"human-il-3-elisa-kit","title":"Human IL-3 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-3 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-3 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-3 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-3 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.94pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e87-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/bae86fbf87614821abca7626b3b56090.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 3 (IL-3) is encoded by the IL3 gene in humans. It is a biological signal (cytokine)It can be used as a part of the immune system to improve the innate immune response to disease. It is secreted by basophils and activated T cells, and can assist the growth and differentiation of bone marrow-derived T cells in the immune response. IL-3 acts by binding to IL-3 receptor, which can stimulate multipotent hematopoietic stem cells to differentiate into myeloid progenitor cells, or together with IL-7, stimulate multipotent hematopoietic stem cells to differentiate into lymphoid progenitor cells. In addition, IL-3 and other cytokines, such as erythropoietin (EPO), granulocyte macrophage colony-stimulating factor (GM-CSF) and IL-6, stimulate the proliferation of all cells of the myeloid system (granulocytes, monocytes and dendritic cells).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306888267,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_061a8397-11ed-4d02-8146-beec00403260.png?v=1770714912"},{"product_id":"human-il-4-high-sensitivity-elisa-kit","title":"Human IL-4 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.03pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.25-16 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e94-105%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/e7257a477a7544d4909754ce034d4d5d.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.\u003c\/p\u003e\n\u003cp\u003eIL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293306953803,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1d2f4f09-d736-4ec1-bd33-5a1a291da2e9.png?v=1770714912"},{"product_id":"human-il-5-elisa-kit","title":"Human IL-5 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-5 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-5 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-5 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-5 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.76pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e91-107%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/900491d1f87648689e411cd74c6786b6.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 5 (IL-5) is a Th2 cytokine member of hematopoietic family, which is composed of 115 amino acids. It is similar to other members of this cytokine family (IL-3 and GM-CSF)Different, the activated form of this glycoprotein is homodimer. IL-5 is secreted by Th2 cells and mast cells. Its biological activity on B cells and other types of cells shows that IL-5 has an important functional effect on the activity of Th2 cells. IL-5 stimulates B cell growth and increases immunoglobulin secretion by binding to its receptor. It is also a key factor in activating eosinophils. IL-5 has been associated with a variety of allergic diseases, including allergic rhinitis and asthma. A large increase in IL-5 was observed in circulating tissues and tracheal tissues, while sputum eosinophils decreased.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307019339,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_da62cc0c-98c9-4add-b630-70e25f571f6c.png?v=1770714910"},{"product_id":"human-il-6-high-sensitivity-elisa-kit","title":"Human IL-6 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-6 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-6 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-6 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-6 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.02pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.31-20 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e74-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/d94a1ed7ee6b4e64be09ac571f23b62a.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 6 (IL-6)It is a cytokine with both pro-inflammatory and anti-inflammatory properties. In humans, it is encoded by the IL-6 gene. During infection and after trauma, especially inflammation caused by burn or other tissue damage, IL-6 will be secreted by T cells and macrophages to promote the immune response. Studies have shown that IL-6 is required for mice to resist Streptococcus pneumoniae infection, indicating that IL-6 plays a role in resisting infection.\u003c\/p\u003e\n\u003cp\u003eIL-6 can promote the process of inflammatory and autoimmune diseases in many diseases, such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. Patients with advanced \/ metastatic cancer have higher levels of IL-6 in their blood.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307084875,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d98f797d-32b4-4ea8-b84d-a23c4e81dc42.png?v=1770714909"},{"product_id":"human-il-8-high-sensitivity-elisa-kit","title":"Human IL-8 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-8 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. IL-8 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-8 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-8 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.28pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e2.34-150 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e91-114%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/71fcd98314a6440bba6758e1dcf1f30d.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 8 (IL-8)It is a chemokine produced by macrophages and other types of cells, such as epithelial cells, airway smooth muscle cells and endothelial cells. In humans, it is encoded by the IL8 gene. IL-8 can effectively promote angiogenesis and play an important role in the pathogenesis of bronchiolitis. IL-8 can regulate the inflammatory response through the recruitment and degranulation of neutrophils, and can act on colorectal cancer as an autocrine growth factor of colon cancer cell lines. High levels of IL-8 can reduce the positive reaction of antipsychotics in patients with schizophrenia. In addition, IL-8 is also associated with the pathogenesis of obesity and cystic fibrosis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307117643,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_bdc0490c-186f-41f8-b0b0-c17650814e84.png?v=1770714909"},{"product_id":"human-il-9-elisa-kit","title":"Human IL-9 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-9 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-9 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-9 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-9 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.06pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81 -500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e80-117%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210129\/9df8b391113d4dbb91d57ec49a4af1fe.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 9 (IL-9)It is a glycosylated protein with a molecular weight of 14kda and contains 10 cysteine residues involved in disulfide bonding. It is a cytokine produced by T cells, especially cd4+ helper cells, and can regulate a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It acts by binding to the IL-9 receptor and can activate different signal transduction and activation (STAT) proteins, thus participating in a variety of biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies of asthma in mouse models have shown that this cytokine is a decisive factor in the pathogenesis of bronchial hyperresponsiveness. The presence of IL-9-mediated autocrine loops implies the presence of some malignancies such as Hodgkin's disease. IL-9 is expressed by Lee Stokes cells, Hodgkin lymphoma cells and some large aplastic lymphoma cells, but not by non Hodgkin lymphoma and peripheral T-cell lymphoma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307183179,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1d1e458e-8cd1-48f8-903e-9b78abd728e2.png?v=1770714908"},{"product_id":"human-il-9-high-sensitivity-elisa-kit","title":"Human IL-9 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-9 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-9 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-9 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-9 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.16pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.63-40 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e98-116%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210207\/6d3dcd391c9b4c46889ce5e038bbdf67.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 9 (IL-9)It is a glycosylated protein with a molecular weight of 14 kDa and contains 10 cysteine residues involved in disulfide bonding. It is a cytokine produced by T cells, especially cd4+ helper cells, and can regulate a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It acts by binding to the IL-9 receptor and can activate different signal transduction and activation (STAT) proteins, thus participating in a variety of biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies of asthma in mouse models have shown that this cytokine is a decisive factor in the pathogenesis of bronchial hyperresponsiveness. The presence of IL-9-mediated autocrine loops implies the presence of some malignancies such as Hodgkin's disease. IL-9 is expressed by Lee Stokes cells, Hodgkin lymphoma cells and some large aplastic lymphoma cells, but not by non Hodgkin lymphoma and peripheral T-cell lymphoma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307215947,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_cf99df23-450e-44e2-a119-0e6c74b73a3d.png?v=1770714908"},{"product_id":"human-gdf-15-elisa-kit","title":"Human GDF-15 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti human GDF-15 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The GDF-15 present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of GDF-15 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, GDF-15 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.24pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e79-115%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRtorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210207\/c4ae268f39f241ef9e5fdd65f0981685.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGrowth differentiation factor 15 (GDF-15), also known as tgf-pl, MIC-1, PDF, plab and ptgfb, are transforming growth factor beta; A member of the superfamily, which plays a role in regulating inflammatory and apoptotic pathways in damaged tissues and disease processes. Gdf-15mrna mainly exists in the liver, and a small amount also exists in some other tissues. In the injury of organs such as liver, kidney, heart and lung, the expression of GDF-15 in liver is significantly up-regulated.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307281483,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_5f1c8b14-4f90-4c56-a29a-49a0247270ba.png?v=1770714906"},{"product_id":"human-scd14-elisa-kit","title":"Human sCD14 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti human sCD14 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The sCD14 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of sCD14 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, sCD14 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e2.58pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e125-8000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e96-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210207\/85f9c4223bc445c2aea0d665c79b002a.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCD14 is mainly produced by macrophages and neutrophils, but also by dendritic cells. It is a component of the innate immune system as a recognition of bacterial lipopolysaccharide (LPS)Of the coreceptor. CD14 can bind LPS only in the presence of lipopolysaccharide binding protein. There are two forms of CD14. One is mCD14, which is anchored to the cell membrane by glycosylphosphatidylinositol, and the other is sCD14, which is a soluble form. SCD14 appears after the shedding of mCD14 (48kDa), or is directly produced by intracellular vesicle secretion (56kda). SCD14 is secreted by the liver and monocytes. At low concentrations, it is sufficient for cells that do not express CD14 to respond to LPS. MCD14 and sCD14 are also present in intestinal epithelial cells. SCD14 is also present in human milk and can regulate the growth of intestinal microbes in infants.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307314251,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_653d9be7-84ce-4299-ba45-c574dfc3d7bc.png?v=1770714905"},{"product_id":"human-rage-elisa-kit","title":"Human RAGE ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti human rage capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for a 2-hour incubation process. Rage in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of rage in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, rage detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.75pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Tange: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e89-108%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210207\/0818baf67a864956a81d6cec4fb53462.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReceptor for advanced glycation end products (RAGE)Is a 35kDa immunoglobulin superfamily transmembrane receptor, whose name comes from its ability to bind advanced glycation end products. Given its inflammatory function in innate immunity and its ability to recognize a class of ligands through common motifs, rage is generally considered a class of recognition receptors. Rage is associated with some chronic diseases, such as atherosclerosis, peripheral vascular disease, myocardial infarction, congestive heart failure, diabetes, Alzheimer's disease, etc. Compared with other tissues, rage has the highest expression in the lung, especially in type I alveolar epithelial cells, but not in idiopathic pulmonary fibrosis (IPF), suggesting that the expression and regulation of rage in the lung system is different from that in the cardiovascular system. Blocking \/ knocking down rage will lead to impaired cell adhesion and increased cell proliferation and migration.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307412555,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e4efb056-662d-4619-bf42-b0bea993136d.png?v=1770714907"},{"product_id":"human-growth-hormone-elisa-kit","title":"Human Growth Hormone ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ePrinciple of detection:\u003c\/strong\u003e\u003cbr\u003eThe double antibody sandwich ELISA technique was used in this kit. Specific anti human growth hormone \/ GH capture antibodies were precoated on high affinity microplate plates. Microplate wells were supplemented with standards and samples to be tested, shaken well to mix and placed at room temperature for a 2-hour incubation, with growth hormone \/ GH present in the sample bound to the solid-phase antibody. After extensive washing to remove unbound components, biotinylated detection antibody was added for incubation. After another wash, horseradish peroxidase labeled streptavidin (streptavidin HRP, SA-HRP) was added. After another extensive wash, TMB chromogenic substrate was added and color was developed protected from light. The depth and lightness of the color reaction had a positive correlation with the concentration of Growth Hormone \/ GH in the sample. The reaction was stopped by adding stop solution, and the absorbance values were determined using a microplate reader at 450 nm detection wavelength (corrected wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eType of detection: \u003c\/strong\u003edouble anti sandwich assay\u003cbr\u003e\u003cstrong\u003eFormat:\u003c\/strong\u003epre coated 96 well plates\u003cbr\u003e\u003cstrong\u003eTest sample type:\u003c\/strong\u003ecell supernatant, serum, plasma\u003cbr\u003e\u003cstrong\u003eLoading: \u003c\/strong\u003e100 μ l\u003cbr\u003e\u003cstrong\u003eKit components:\u003c\/strong\u003epre coated 96 well plates, standards, GH detection antibodies, standard dilutions, assay buffer, TMB chromogenic substrate, wash buffer, stop solution, sa-hrp, plate sealing membrane and one copy of the instructions.\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e0.62 pg \/ ml\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e62.5-4000 pg \/ ml\u003cbr\u003e\u003cstrong\u003eRecovery range:\u003c\/strong\u003e80-124%\u003cbr\u003e\u003cstrong\u003eMethod of preservation:\u003c\/strong\u003e2-8 ° C\u003cbr\u003e\u003cstrong\u003eStandard curve plots:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210207\/c23453724bef49318639d168c615c95b.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e\u003cbr\u003eIn humans and other animals, growth hormone (Growth Hormone \/ GH) stimulates growth, cell reproduction, and cell regeneration. Therefore it is important for human development. Growth hormone is a stress hormone that elevates the concentrations of glucose and free fatty acids. It also promotes IGF-1 production. The most common condition of growth hormone excess is pituitary tumor. The effects produced by growth hormone deletion differed by age. For young children, growth arrest and short stature are the main manifestations of growth hormone deficiency, often due to genetic factors and congenital malformations. It can also lead to delayed sexual maturation. In adults, GH deficiency is rare, most commonly due to pituitary tumors, other causes including continuation of GH deficiency in childhood, other structural damage or trauma, and extremely rare cases of idiopathic GH deficiency (GHD).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307510859,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_481aa2d5-d6ec-4d38-95ea-684000b1c75c.png?v=1770714904"},{"product_id":"human-lif-elisa-kit","title":"Human LIF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti human LIF capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The LIF existing in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color response has a positive correlation with the concentration of LIF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, LIF detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.64pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e81-117%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/c824b28206af4226b6f70d0c035a982c.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLeukemia inhibitory factor (LIF)It is an interleukin-6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels decrease, cells differentiate. Activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts can all express lif. LIF is named for its ability to induce terminal differentiation of myeloid leukemia cells and prevent the continuous growth of leukemia cells. Other properties of LIF include promoting growth, promoting the differentiation of different types of target cells, affecting bone metabolism, cachexia, neural development, embryonic development and inflammation. LIF is involved in the process of endometrial decidualization and embryo implantation into the endometrium during pregnancy. When the levels of LIF and other cytokines in women drop, they are prone to pregnancy, but the risk of unexplained recurrent abortion will increase. Studies have shown that recombinant human LIF may help improve the implantation rate of female unexplained infertility. In addition, LIF is usually added to stem cell culture medium to reduce spontaneous differentiation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307805771,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_46dc9caa-8ec6-46c8-b917-d348a763840f.png?v=1770714904"},{"product_id":"human-bmp-2-elisa-kit","title":"Human BMP-2 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human BMP-2 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. BMP-2 in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color response has a positive correlation with the concentration of BMP-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, BMP-2 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.73pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e80-121%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/9048844024da41229d7f5ab9c0647261.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eBone morphogenetic protein 2 (BMP-2)Belongs to tgf-β Superfamily proteins. Like other bone morphogenetic proteins, BMP-2 plays an important role in the development of bone and cartilage. BMP-2 participates in Hedgehog pathway and tgf-β The interaction between signaling pathways and cytokines and their receptors is also involved in cardiomyocyte differentiation and epithelial to mesenchymal cell transformation. BMP-2 and BMP-7 are both osteoinductive bone morphogenetic proteins, and experiments have shown that they can effectively induce the differentiation of osteoblasts in many cell types. The use of biconical threaded fusion cage and recombinant human BMP-2 on absorbable collagen sponge can maintain the fusion of intervertebral spine, improve the clinical efficacy, and reduce the pain of patients with degenerative lumbar disc disease after anterior lumbar interbody fusion.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307838539,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_b136237a-0302-4ee4-9ed0-1c6671ef98f3.png?v=1770714904"},{"product_id":"human-il-10-high-sensitivity-elisa-kit","title":"Human IL-10 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.05pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.39-25 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e88-100%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/1071b805420d4ad3affe9b590b8a7dc6.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL10 gene located on chromosome 1 and consists of five exons. IL-10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - \u0026amp; kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293307969611,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_cdb10bfc-f497-4ab5-a21f-592699a5d9e8.png?v=1770714903"},{"product_id":"human-il-11-elisa-kit","title":"Human IL-11 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eTesting Principle：\u003cbr\u003e\u003c\/strong\u003eThis kit uses double antibody sandwich ELISA.\u003cspan class=\"transSent\" data-group=\"0-0\"\u003eSpecific anti-human IL-11 capture antibody is prepackaged on a high affinity conjugate plate. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-1\"\u003eThe standard substance, the sample to be tested and the detection antibody labeled with biotin were successively added into the HRP-plate well. After full oscillation and mixing, the IL-11 in the sample was placed at room temperature for a 2-hour incubation process. The IL-11 in the sample was combined with the solid phase antibody and the detection antibody. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-2\"\u003eAfter washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-3\"\u003eAfter washing again, TMB color substrate was added and incubated in dark at room temperature for color development. \u003c\/span\u003e\u003cspan class=\"transSent highlight\" data-group=\"0-4\"\u003eThe depth of the color reaction was positively correlated with the concentration of IL-11 in the sample. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-5\"\u003eThe termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection type：\u003c\/strong\u003eDouble sandwich enzyme immunoassay\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eForm\u003c\/strong\u003e: pre-coated 96-well plate\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e: Cell culture supernatant, Serum, Plasma\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample quantity：\u003c\/strong\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eKit composition：\u003c\/strong\u003epre-coated 96-well plate,Standards,IL-11 test antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Mask, and Instructions .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSensitivity：\u003c\/strong\u003e5.01 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection range：\u003c\/strong\u003e62.5-4000 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eRecovery rate: \u003c\/strong\u003e84-118%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eStorage：\u003c\/strong\u003e2-8℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eThe standard curve\u003c\/strong\u003e\u003cstrong\u003e：\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/6e6bc4a6f9e54e65a9788002259d7ffe.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eBackground：\u003cbr\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cspan class=\"transSent\" data-group=\"0-0\"\u003eInterleukin 11 (IL-11) is a pluripotent cytokine that was isolated from bone marrow mesenchymal stem cells as early as 1990. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-1\"\u003eIt is a member of the IL-6 cytokine family and can be distinguished according to the co-receptor they use, gp130. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-2\"\u003eIL-11 plays an important regulatory role in hematopoietic regulation, especially in stimulating the maturation of megakaryocytes. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-3\"\u003eStudies have shown that IL-11 can improve chemotherapy-induced laminopenia, induce acute phase protein, regulate antigen-antibody response, and participate in the regulation of proliferation and differentiation of bone cells. \u003c\/span\u003e\u003cspan class=\"transSent highlight\" data-group=\"0-4\"\u003eIL-11 causes bone resorption and also stimulates the growth of certain lymphocytes and, in a mouse model, increases the thickness and strength of the long bone cortex. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-5\"\u003eAs a signaling molecule, IL-11 has a variety of functions, including placental formation and to some extent decidualization, and plays a role in the implantation of blastocysts into the endometrium. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-6\"\u003eIL-11, which is made using recombinant DNA technology and is marketed as Oprexil interleukin, is used to prevent severe thrombocytopenia in cancer patients.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308035147,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9540c12b-2c3f-422c-bba7-79b2e4651f0d.png?v=1770714902"},{"product_id":"human-scd163-elisa-kit","title":"Human sCD163 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti human CD163 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The CD163 present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of CD163 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003edouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, CD163 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e4.39pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e62.5-4000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e88-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/5b51d9c454674fcd9f2675353e6363e1.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCD163 is a protein encoded by the CD163 gene in humans. It belongs to the cysteine rich B-type scavenger receptor family, and contains an extracellular domain consisting of 1048 amino acid residues, a single transmembrane segment, and a cytoplasmic tail region with several splicing variations. It is a hemoglobin binding beadScavenger receptors for protein complexes that label monocyte \/ macrophage lineages. The soluble form of this receptor is present in plasma and is usually expressed as scd163. Scd163 is produced by the ectodomain shedding of membrane-bound receptors and is upregulated in a wide range of inflammatory diseases including liver cirrhosis, type 2 diabetes, macrophage activation syndrome, Gaucher disease, sepsis, HIV infection, rheumatoid arthritis and Hodgkin lymphoma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308067915,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e8d0470b-7a90-4a87-aca4-dbe542b8254d.png?v=1770714902"},{"product_id":"human-galectin-3-elisa-kit","title":"Human Galectin-3 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human galectin-3 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. Galectin-3 in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of galectin-3 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, galectin-3 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e2.46pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e62.5-4000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85-105%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/92fa579dceda42e29249c0eddf33dbc4.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGalectin-3It belongs to the lectin family and is expressed in the nucleus, cytoplasm, mitochondria, cell surface and extracellular. Galectin-3 pair \u0026amp;beta-Galactosidase has affinity and exhibits its activity against bacteria and fungi. It is involved in many biological processes, such as cell adhesion, cell activation and chemotaxis, cell growth and differentiation, cell cycle and apoptosis. Based on its extensive biological functions, galectin-3 is considered to be related to cancer, inflammation and fibrosis, heart disease, stroke, etc. Galectin-3 expression levels are associated with various types of fibrosis. Galectin-3 expression is upregulated in liver fibrosis, kidney fibrosis, and idiopathic pulmonary fibrosis (IPF). In acute decompensated heart failure and chronic heart failure populations, high expression of galectin-3 leads to a higher risk of death. Meanwhile, galectin-3 is thought to play an important role in tumor metastasis.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308133451,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_daa3ac11-9478-4f20-9373-8a1e75d12839.png?v=1770714900"},{"product_id":"human-bdnf-elisa-kit","title":"Human BDNF ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti human BDNF capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The BDNF present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of BDNF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, BDNF detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e1.41pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e7.81-500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e85-115%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/993c258d16f74b5cbd83a894daa4192a.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eBrain derived neurotrophic factor (BDNF)It is a member of the neurotrophin family of growth factors and is related to nerve growth factors. Neurotrophins exist in brain and periphery. BDNF is processed and synthesized in the endoplasmic reticulum and secreted by dense core vesicles. It is active in the hippocampus, cortex, and basal forebrain regions of the brain, as well as in the retina, motor neurons, kidneys, saliva, and prostate. BDNF acts on some neurons of the central nervous system and peripheral nervous system, helping the survival of existing neurons and promoting the growth and differentiation of newborn neurons and synapses. The changes of BDNF may play a role in the pathogenesis of schizophrenia, and are related to depression, eczema, epilepsy, Alzheimer's disease, obesity, aging, congenital central hypoventilation syndrome and cognitive dysfunction after chemotherapy,\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308166219,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_76ec8da0-ac3b-4b34-a35c-c1c54f293dfb.png?v=1770714899"},{"product_id":"human-il-12p70-high-sensitivity-elisa-kit","title":"Human IL-12p70 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e Detection principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Human IL-12 capture antibody Pre-coated on high affinity enzyme plates. The standard substance, the sample to be measured and the biotin-labeled detection antibody are sequentially added into the wells of the enzyme label plate, thoroughly shaken and mixed, and then placed at room temperature for time 2 Hours of incubation process, present in the sample IL-12 Binds to solid phase antibodies and detection antibodies. After sufficient washing to remove free and unbound components, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP ， SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-12 The concentration of is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Test Type: \u003c\/strong\u003e Double antibody sandwich method \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Form: \u003c\/strong\u003e Pre-coating 96 Orifice plate \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Test Sample Type: \u003c\/strong\u003e Cell supernatant, serum, plasma \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Sample Load: \u003c\/strong\u003e100 μl\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Kit Components: \u003c\/strong\u003e Pre-coating 96 Well plates, standards, IL-12p70 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Sensitivity: \u003c\/strong\u003e0.04\u003cstrong\u003e \u003c\/strong\u003epg\/mL\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Detection range: \u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e0.39-25 pg\/mL\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Recovery Range: \u003c\/strong\u003e 91-104%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Save Method: \u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Standard graph: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/07fd2a0e7c864b5690a88aba11ea9a8c.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Background: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Interleukin 12(IL-12) Composed of dendritic cells, macrophages, neutrophils and human B Lymphoblastic cell line (NC-37) Arises naturally in response to antigenic stimulation. IL-12 By 4 1 а Helix composed, is a composed of 2 Species independent gene ---IL-12A(p35) And IL-12B(p40) Encoding the formed heterodimeric cytokine. Biologically active heterodimers can be formed after protein synthesis (p70) And homodimers p40 。 IL-12 Participate in initial T Cells to Th1 Process of cell differentiation, as T Cell stimulating factor, which stimulates T The growth of cells and their functions. IL-12 Has anti-angiogenic activity, and is effective against natural killer cells and T Lymphocyte activity plays an important role. Similarly, IL-12 Associated with autoimmunity. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308231755,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f67c5476-6621-4d6d-ba9c-c1bd67d6a92b.png?v=1770714899"},{"product_id":"human-il-17a-high-sensitivity-elisa-kit","title":"Human IL-17A High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A\/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eTesting Principle：\u003c\/strong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eThis kit uses double antibody sandwich ELISA.Specific anti-human IL-17A capture antibody is precoated on a high affinity conjugate plate.  The standard substance, the sample to be tested and the detection antibody labeled with biotin were successively added into the HRP-plate well. After fully shaking and mixing, the IL-17A in the sample was placed at room temperature for a 2-hour incubation process. The IL-17A in the sample was combined with the solid phase antibody and the detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of the color reaction was positively correlated with the concentration of IL-17A in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection type：\u003c\/strong\u003eDouble sandwich enzyme immunoassay\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eForm\u003c\/strong\u003e: pre-coated 96-well plate\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e: Cell culture supernatant, Serum, Plasma\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample quantity：\u003c\/strong\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eK\u003c\/strong\u003e\u003cstrong\u003eit composition：\u003c\/strong\u003epre-coated 96-well plate,Standards,IL-12P70 Detection Antibody, Standard Diluent, Detection Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substent, Washing Solution, Terminating Solution, SA-HRP, Sealing Plate Mask and Instructions  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSensitivity：\u003c\/strong\u003e0.20 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection range：\u003c\/strong\u003e0.78-50 pg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eRecovery rate：\u003c\/strong\u003e80-95%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eStorage：\u003c\/strong\u003e2-8℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eThe standard curve\u003c\/strong\u003e\u003cstrong\u003e：\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/ee4c7cd93141481386da16f11ec81247.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eBackground：\u003cbr\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cspan class=\"transSent\" data-group=\"0-0\"\u003eInterleukin 17A(IL-17 or IL-17A), a representative member of the IL-17 family of cytokines, is produced by helper T lymphocytes and can be induced by IL-23. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-1\"\u003eIL-17 cytokines can effectively recruit monocytes and neutrophils to inflammatory sites by increasing the production of chemokines in tissues, thereby delaying the occurrence of inflammation. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-2\"\u003eWhen an extracellular pathogen invades the immune system, IL-17, a pro-inflammatory cytokine, responds by destroying the pathogen's cells. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-3\"\u003eIL-17 exerts synergistic effects with tumor necrosis factor and IL-1. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-4\"\u003eThe IL-17 family is associated with a number of immune\/autoimmune-related diseases, such as rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumor immunity, psoriasis, multiple sclerosis.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308330059,"sku":null,"price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_fbf697f8-5c9c-49bf-80b4-ab6a76860d4d.png?v=1770714898"},{"product_id":"human-il-22-high-sensitivity-elisa-kit","title":"Human IL-22 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-22 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-22 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-22 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-22 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.67pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e6.25-400 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e92-117%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/e32c0742dfb44a55bfa3b720a3cd2b57.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 22 (IL-22) is an IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26)As a member of cytokines, it is an effective regulator of cellular inflammatory response. It uses il-10r2 together with other members of this family, IL-10, IL-26, il-28a\/b, and IL-29, in cell signaling. IL-22 is produced by activated DCs and T cells, which initiate innate immune responses against pathogenic bacteria, especially epithelial cells derived from the respiratory tract and gut. The plasma of psoriasis patients showed strongly elevated IL-22 levels, which correlated with the severity of the disease. In T cell-mediated hepatitis induced by concanavalin A (Con A), IL-22 can play a protective role as a survival factor of hepatocytes. With ifn-γ In contrast to IL-17, there is a large amount of IL-22 in the blood of patients with Crohn's disease\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308362827,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_2e8e9565-82c0-4337-9416-71efca464807.png?v=1770714898"},{"product_id":"human-il-29-high-sensitivity-elisa-kit","title":"Human IL-29 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-29 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-29 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-29 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-29 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.08pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e3.13-200 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e86-108%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/1515c0357a244d2390afe6ade7e27811.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 29 (IL-29), also known as interferon λ 1 (ifn-λ 1), a member of the helical cytokine family, is a type III interferon. Its amino acid sequence is highly similar to that of Il-28, another type III interferon. The IL-29 gene is found on human chromosome 19, but it does not exist in the mouse genome. IL-29 plays an important role in host defense against microorganisms, and its gene is highly upregulated in virus-infected cells. IL-29 has significant antiviral activity and immunomodulatory properties, which can be similar to ifn-\u0026amp;alpha through induction\/Β Activated cellular antiviral response to inhibit virus replication. However, IL-29 is bound to a unique receptor, so in the process of natural infection, this cytokine may interact with ifn-\u0026amp;alpha\/Β Or ifn-γ It may also work together with other cytokines to treat chronic viral infections such as hepatitis C (HCV). The antiviral activity of IL-29 includes the upregulation of class I MHC expression on the cell surface and the upregulation of PKR expression. The ligand \/ receptor complex appears to signal through the JAK STAT pathway.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308559435,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_ce35e692-428a-4728-99c1-1f79516eb71f.png?v=1770714898"},{"product_id":"human-il-33-high-sensitivity-elisa-kit","title":"Human IL-33 High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-33 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-33 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-33 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-33 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.16pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e6.25-400 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e92-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/02dc3e5711554776aeea8b198d50c245.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 33 (IL-33)Also known as nf-hev and DVS 27, it is a member of the IL-1 family with a molecular weight of 30 kDa and is secreted by many types of cells, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 can induce the secretion of type II cytokines by helper T cells, mast cells, eosinophils and basophils. IL-33 activates NF - \u0026amp; kappa by binding to receptor ST2 and IL-1 receptor accessory protein (IL1RAP); B and extracellular molecules in the MAP kinase signaling pathway, the latter can promote polarized Th2 cells to secrete type II cytokines (such as IL-5 and IL-13), thereby exerting their biological functions. After injection of IL-33, it can induce type II cytokines in vivo, which is thought to induce severe pathological changes in mucosal organs. The ligand \/ receptor complex appears to signal through the JAK STAT pathway.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308624971,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4a8e8cfa-7239-4d88-b246-90c9bdd3fbd8.png?v=1770714897"},{"product_id":"human-gm-csf-high-sensitivity-elisa-kit","title":"Human GM-CSF High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti human GM CSF capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The GM-CSF in the sample is combined with the solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of GM-CSF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, GM-CSF detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.07pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.16-10 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e80-101%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/9e7f2074e9a340c4a656d28e7db788b1.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte macrophage colony stimulating factor (GM-CSF) is a cytokine that regulates the growth of leukocytes. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils)And monocytes. In addition, it is also active against hematopoietic and non hematopoietic lineage cells. GM-CSF is used as a drug to stimulate the production of leukocytes, thereby preventing neutropenia caused by chemotherapy. Recently, people are conducting clinical trials to evaluate the efficacy of GM-CSF as a potential vaccine adjuvant for HIV infected patients. Relevant studies have reported that some patients with cancer (including lung cancer and acute myeloid leukemia) have higher levels of GM-CSF in the serum, and also have higher levels of GM-CSF in the peripheral blood and scale extracts of patients with bronchial asthma.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308657739,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_79aa07b5-c980-44e3-b2c6-a1f4e667ccb7.png?v=1770714896"},{"product_id":"human-g-csf-high-sensitivity-elisa-kit","title":"Human G-CSF High Sensitivity ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti human G-CSF capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The G-CSF in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of G-CSF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, G-CSF detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.19 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e94-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/45455d3f7a594280b8f7bfcc7256e06c.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGranulocyte colony stimulating factor (G-CSF)It is a glycoprotein that stimulates bone marrow to produce granulocytes and stem cells and release them into the blood. Functionally, it is a cytokine and hormone, a colony stimulating factor, produced by endothelial cells, macrophages and many other immune cells. G-CSF stimulates the survival, proliferation, differentiation and function of neutrophil precursors and mature neutrophils. In oncology and hematology, recombinant G-CSF is used to accelerate the recovery of neutropenia caused by chemotherapy in cancer patients. Before leukocyte removal, G-CSF can also be used to increase the number of hematopoietic stem cells in the blood of blood donors for hematopoietic stem cell transplantation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308723275,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9583db6d-6d96-40a4-abe3-d052a667d75c.png?v=1770714897"},{"product_id":"human-il-31-elisa-kit","title":"Human IL-31 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. Specific anti-human IL-31 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-31 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-31 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-31 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.78 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e15.63-1000 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e84-117%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/8045508fa03d4455b690873125cbf53e.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 31 (IL-31)Yes α A short chain member of the helical cytokine family with a molecular weight of 24 kDa, which is mainly associated with activated T cells, is preferentially expressed by Th2 cells rather than Th1 cells. Human IL-31 cDNA encodes a 164 amino acid precursor, which includes a 23 amino acid signal peptide and a mature protein containing 141 amino acids. Human and mouse IL-31 share 24% amino acid sequence homology in the mature region. IL-31 generates signals through a receptor complex, which consists of IL-31 receptor A (IL-31Ra) and tumor suppressor M receptor subunits. These receptor subunits are expressed in activated monocytes and unstimulated epithelial cells. IL-31 signaling is involved in jak\/stat pathway, PI3 kinase \/akt signaling cascade and MAP kinase pathway. In addition, IL-31 plays a role in skin inflammation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308919883,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4c9c1e11-a760-4e86-990d-8b6152a57e3d.png?v=1770714896"},{"product_id":"human-il-33-elisa-kit","title":"Human IL-33 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eC9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTesting Principle：\u003c\/strong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eThis kit uses double antibody sandwich ELISA.Specific anti-human IL-33 capture antibody is prepackaged on a high affinity conjugate plate.  The standard substance, the sample to be tested and the detection antibody labeled with biotin were successively added into the HRP-plate well. After fully shaking and mixing, the IL-33 in the sample was placed at room temperature for a 2-hour incubation process. The IL-33 in the sample was combined with the solid phase antibody and the detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of the color reaction was positively correlated with the concentration of IL-33 in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection type：\u003c\/strong\u003eDouble sandwich enzyme immunoassay\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm\u003c\/strong\u003e: pre-coated 96-well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e: Cell culture supernatant, Serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample quantity：\u003c\/strong\u003e100 μl\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit composition：\u003c\/strong\u003epre-coated 96-well plate,Standards,IL-33 test antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Mask and Instructions  \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity：\u003c\/strong\u003e2.17 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range：\u003c\/strong\u003e31.25-2000 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery rate：\u003c\/strong\u003e80-116%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage：\u003c\/strong\u003e2-8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe standard curve\u003c\/strong\u003e\u003cstrong\u003e：\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/4826adcc414e40f9a001736a6f4e0ebd.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground：\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"transSent highlight\" data-group=\"0-0\"\u003eInterleukin 33 (IL-33), also known as NF-HEV and DVS 27, is a member of the IL-1 family with a molecular weight of 30 kDa and is secreted by several cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-1\"\u003eIL-33 induces the secretion of type II cytokines by helper T cells, mast cells, eosinophils, and basophils. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-2\"\u003eIL-33 activates extracellular molecules in the NF-κB and MAP kinase signaling pathways by binding to the receptor ST2 and IL-1 receptor helper protein (IL1RAP), which induces the secretion of type II cytokines (such as IL-5 and IL-13) by polarized Th2 cells. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-3\"\u003eTo unleash its biological function. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-4\"\u003eAfter IL-33 is injected, it induces type II cytokines in vivo, which are thought to induce severe pathological changes in mucosal organs.  \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293308952651,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_bb8c6608-3ea7-48dd-88bf-22b6b82ee951.png?v=1770714894"},{"product_id":"human-il-35-elisa-kit","title":"Human IL-35 ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA technology. The specific anti-human IL-35 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-35 in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-35 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, IL-35 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e76.93 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e0.47-30 ng\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e95-119%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/1bf65cca48a242c5b3fcc8b538a12312.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eInterleukin 35 (IL-35)Cytokines produced by regulatory rather than effector T cells, a member of the IL-12 family, play a role in immunosuppression. It is composed of il-12α Chain and il-27β A dimeric protein composed of chains, encoded by two independent genes, il-12a and ebi3, respectively. IL-35 secreted by regulatory T cells (Tregs) inhibits the inflammatory response of immune cells. IL-35 is not continuously expressed in tissues, but after the activation of inflammatory stimuli, the gene encoding IL-35 is transcribed by vascular endothelial cells, smooth muscle cells and monocytes. Studies in mice have shown that the deletion of IL-35 chain reduces the ability of cells to suppress inflammation, which has also been observed in cell culture experiments and experimental models of inflammatory bowel disease. In order to produce inhibitory effect, IL-35 has selective activity on different T cell subsets. It can induce the proliferation of Treg cell population and reduce the activity of Th17 cell population.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293309018187,"sku":null,"price":483.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a1c4c260-3871-4fa9-bc46-ba04bfb68cf6.png?v=1770714894"},{"product_id":"human-igg-elisa-kit","title":"Human IgG ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit uses double antibody sandwich ELISA detection technology. Specific anti human IgG capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the microplate plate for incubation. After incubation, the IgG present in the sample binds to the solid-phase antibody. After washing to remove unbound material, horseradish peroxidase (HRP) - labeled detection antibody was added for incubation. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color reaction is directly proportional to the concentration of IgG in the sample. The reaction was stopped by adding stop solution, and the absorbance value was measured at 450 nm wavelength (correction wavelength 570 - 630 nm).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003epre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Sample Type: \u003c\/strong\u003ecell supernatant, serum, plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100 μ L\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003eA copy of pre coated 96 well plate, standard, HRP labeled IgG detection antibody, detection buffer, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e0.18 ng\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e3.13-200 ng\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e93-110%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/61a951e1cd4f46d1984628509defe435.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eImmunoglobulin G (IgG)Is an antibody, which is a protein complex composed of four peptide chains \u0026amp;mdash\u0026amp;Mdash; A Y-type antibody monomer consisting of two identical heavy chains and two identical light chains. Each IgG has 2 antigen binding sites. IgG is the main antibody of blood and extracellular fluid, which can control the infection of human tissues. Plasma B cells can produce and release IgG molecules.\u003c\/p\u003e\n\u003cp\u003eIgG is the only immunoglobulin that can pass through the human placenta, thus providing protection for the fetus in utero. IgG antibodies extracted from the plasma of blood donors can be used as intravenous immunoglobulins to treat immunodeficiency, autoimmune diseases and infectious diseases.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293309116491,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f77a5954-5d8a-449b-ae35-6729d994ec1f.png?v=1770714893"},{"product_id":"human-iga-elisa-kit","title":"Human IgA ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eImmunoglobulin A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eTesting Principle：\u003c\/strong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eThis kit uses double antibody sandwich ELISA.Specific anti-human IgA capture antibody is prepackaged on a high affinity conjugate plate.  Standard substance, test sample and detection antibody labeled with horseradish peroxidase were added into the HRP-plate well. After incubation, IgA in the sample was combined with solid phase antibody and detection antibody.  After washing, color substrate TMB is added to protect the color from light.  The depth of the color reaction is proportional to the concentration of IgA in the sample.  A termination solution was added to terminate the reaction and the absorbance was measured at 450 nm (correction wavelength 570-630 nm).  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection type：\u003c\/strong\u003eDouble sandwich enzyme immunoassay\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eForm\u003c\/strong\u003e: pre-coated 96-well plate\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e: Cell culture supernatant, Serum, Plasma\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSample quantity：\u003c\/strong\u003e100 μl\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eKit composition：\u003c\/strong\u003epre-coated 96-well plate,Standards,HRP-labeled IgA test antibody, test buffer, TMB color substrate, washing solution, stop solution, sealing plate membrane and instruction manual  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eSensitivity：\u003c\/strong\u003e0.35 ng\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eDetection range：\u003c\/strong\u003e3.13-200 ng\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eRecovery rate：\u003c\/strong\u003e90-108%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eStorage：\u003c\/strong\u003e2-8℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eThe standard curve\u003c\/strong\u003e\u003cstrong\u003e：\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003e \u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20210208\/95a9fb6363d1411e87204f4e03f9d634.png\" alt=\"\" width=\"300\" height=\"300\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cstrong\u003eBackground：\u003cbr\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003e\u003cspan class=\"transSent\" data-group=\"0-0\"\u003eImmunoglobulin A(IgA) is an antibody that plays an important role in mucosal immunity. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-1\"\u003eIts heavy chain is the alpha subtype. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-2\"\u003eThere are two subclasses of IgA, IgA1 and IgA2, which exist as secretory IgA(sIgA) dimer. \u003c\/span\u003e\u003cspan class=\"transSent highlight\" data-group=\"0-3\"\u003eIn mucosal secretions, SIgA is the most important immunoglobulin, which is less present in blood. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-4\"\u003eThe secretory component of SIgA protects immunoglobulin from proteolytic enzyme degradation, so SIgA can exist in harsh gastrointestinal environment and inhibit microbial reproduction in body secretions. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-5\"\u003eSIgA also inhibits the inflammatory effects of other immunoglobulins. \u003c\/span\u003e\u003cspan class=\"transSent\" data-group=\"0-6\"\u003eThe activation and regulation of complement system by IgA is weak.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293309149259,"sku":null,"price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_cb73dbaa-f680-477f-a6fc-65abf1a75ef2.png?v=1770714892"}],"url":"https:\/\/www.antbioinc.com\/collections\/abs5.oembed","provider":"AntBio","version":"1.0","type":"link"}