{"title":"261113-BF HOT PICKS","description":null,"products":[{"product_id":"human-liver-organoid-culture-medium-kit","title":"Human Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human liver primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal liver organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal liver organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal liver organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e Human normal liver organoid culture medium is a culture medium that promotes the formation and amplification of human normal liver organoids in vitro. This product is a sterile liquid mixture system containing amino acids, vitamins, organic and inorganic compounds, and growth factors essential for maintaining the growth of the target cells. The unique formulation of this medium can provide a suitable nutritional environment required by cells to promote the in vitro growth and expansion of normal human liver organoids. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cstrong\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 72.89%; height: 176px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e human normal liver organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e digestive fluid of human normal liver primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303119947,"sku":"abs9544-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e6c0b549-5e5c-4416-bc6d-f18971c173cd.jpg?v=1792926065"},{"product_id":"human-lung-organoid-culture-medium-kit","title":"Human Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human lung primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal lung organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal lung organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal lung organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303152715,"sku":"abs9546-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e8cdcc9e-c7c5-4c31-8144-724f40f13146.jpg?v=1792926067"},{"product_id":"human-pancreatic-organoid-culture-medium-kit","title":"Human Pancreatic Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary pancreatic tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal pancreatic organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal pancreatic organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal pancreatic organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal pancreatic organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal pancreatic primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303218251,"sku":"abs9547-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_b81d8a9f-0491-41bc-8d9b-e02746b018a4.jpg?v=1792926070"},{"product_id":"mouse-intestinal-cancer-organoid-culture-medium-kit","title":"Mouse Intestinal Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse intestinal cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse intestinal cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse intestinal carcinoma organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse intestinal carcinoma organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse intestinal carcinoma organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse intestinal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303251019,"sku":"abs9548-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_a9e9a4c4-b3a5-42f8-b340-f02d97398764.jpg?v=1792926072"},{"product_id":"mouse-gastric-cancer-organoid-culture-medium-kit","title":"Mouse Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse gastric cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse gastric cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse gastric cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse gastric cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse gastric cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse gastric cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303316555,"sku":"abs9549-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ae8cc6df-53cf-4100-be50-828226620f47.jpg?v=1792926074"},{"product_id":"mouse-lung-cancer-organoid-culture-medium-kit","title":"Mouse Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse lung cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse lung cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL mouse lung cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL mouse lung cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 95%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse lung cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of mouse lung cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303349323,"sku":"abs9550-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_3ad054f4-9bf0-4998-96fd-c32891f9f798.jpg?v=1792926077"},{"product_id":"mouse-breast-cancer-organoid-culture-medium-kit","title":"Mouse Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse breast cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse breast cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse breast cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse breast cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse breast cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse breast cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303414859,"sku":"abs9551-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_a230fe95-71ed-4464-90fb-b39aa429013d.jpg?v=1792926080"},{"product_id":"mouse-hepatocarcinoma-organoid-culture-medium-kit","title":"Mouse Hepatocarcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of mouse liver cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of mouse liver cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse liver cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse liver cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse liver cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse liver cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303447627,"sku":"abs9552-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_3d6e4a80-f995-4e2b-822c-11f0cc2d1465.jpg?v=1792926083"},{"product_id":"mouse-pancreatic-cancer-organoid-culture-medium-kit","title":"Mouse Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of pancreatic cancer in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse pancreatic cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Mouse pancreatic cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Mouse pancreatic cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse pancreatic cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of mouse pancreatic cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303513163,"sku":"abs9553-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_da21bbd8-3d93-429a-9956-94f2cc7c90dc.jpg?v=1792926084"},{"product_id":"bovine-mammary-glands-organoid-culture-medium-kit","title":"Bovine Mammary Glands Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary mammary tissue of dairy cows for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, adding dairy cow mammary gland organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul dairy cow mammary gland organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul dairy cow mammary gland organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e dairy cow mammary gland organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive juice of primary mammary tissue of dairy cow  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303873611,"sku":"abs9591-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/58322a37c6ab4b6eabc91acf0566c768.png?v=1792926105"},{"product_id":"mouse-gastric-organoid-culture-medium-kit","title":"Mouse Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary gastric tissue in mice for tissue use  C  digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal gastric organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL mouse normal gastric organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL mouse normal gastric organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Gastric Organoid Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse normal gastric organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal gastric primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303906379,"sku":"abs9643-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e3ccba15-bc33-402d-8ebf-0ec94d5b0f72.jpg?v=1792926108"},{"product_id":"human-gliomatosis-cerebri-organoid-culture-medium-kit","title":"Human Gliomatosis Cerebri Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human glioma primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human glioma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human glioma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human glioma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human glioma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human glioma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293303971915,"sku":"abs9739-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_4cc99c18-0ca5-4e0a-bb8e-11ba0660ddb1.jpg?v=1792926115"},{"product_id":"human-prostate-cancer-organoid-culture-medium-kit","title":"Human Prostate Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human prostate cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25 For μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human prostate cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human prostate cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human prostate cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human prostate cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human prostate cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304004683,"sku":"abs9743-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_6c819dcc-7209-4986-b13c-adf630b2ce57.jpg?v=1792926119"},{"product_id":"mouse-ovary-organoid-culture-kit","title":"Mouse ovary Organoid  Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 Normal ovarian tissue from mice after sampling must be in  2 - 8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3 mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 Digestive fluid of normal ovarian primary tissue in mice for tissue use C digestion, 37℃ oscillation digestion 15-20 min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer B Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer B Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25-30 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal ovary organoid medium A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500uL mouse normal ovarian organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL mouse normal ovarian organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 64.8752%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e mouse normal ovarian organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal ovarian primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 12.526%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41293304070219,"sku":"abs9754-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_b8c6da4e-955d-43ed-8209-662e6276c75b.jpg?v=1792926120"},{"product_id":"human-il-2-onestep-elisa-kit","title":"Human IL-2 OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 5.9%；\u003cbr\u003eInter-assay: 8.5%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e5 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e15.6 pg\/ml - 1000 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eCell culture supernatant\t112% ; Serum\t103%; Plasma\t118%\t\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ehuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eThe dose response curve of the International Standard (86\/500) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (86\/500) units, use the following equation: NIBSC (86\/500) approximate value (IU\/mL) = 0.014 x OneStep Human IL-2 value (pg\/mL).\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eInterleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against \"self-antigens\", which could result in \"autoimmunity\". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293357678667,"sku":"S0C3001-1x96T","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1fedb75b1e0e4c14a07e404c2b02281f_fb065335-8703-4ee4-a5e3-2e33b49e2878.png?v=1794567648"},{"product_id":"rabbit-igg-onestep-elisa-kit","title":"Rabbit IgG OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 3.7%;\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 8.2%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.036 ng\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.156 ng\/ml - 10 ng\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant\t80%\t\u003cbr\u003eSerum\t113%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003erabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\/\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThe rabbit immune system generates antibody diversity and optimizes affinity by mechanisms different from those of mice and other rodents. Compared with traditional mouse mAbs, the rabbit mAbs have advantages in diagnostics with high affinity and specificity toward antigens, more diverse epitope recognition, and greatly improved response to small-size epitopes and mouse antigens. The rabbit immunoglobulin heavy chain (IGH) locus contains over 200 IGH variable germline genes, with over 50% have been found to be “non-functional”. Also, more than 50 IG Kappa V and 17 IG Lambda V functional genes were identified with genomic sequencing (IMGT database). The diversity of rabbit VH repertoire is more limited, and the V light (VL) repertoire is more diverse than that in mice and humans. Most rabbit antibodies are derived from the IGHV1 gene. In addition, the rabbit VL repertoire displays a larger complementarity-determining region (CDR)-L3 loop length than its human and mouse counterparts. Rabbit serum and plasma samples contain around 10 mg\/ml of IgG. The use of hybridoma technology or other platforms (e.g., phase display, single B cell antibody technology) for the production of rabbit monoclonal antibodies has created the requirement for a fast and simple procedure for quantifying antibody production.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293357711435,"sku":"S0C3002-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41677684932683,"sku":"S0C3002-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677684965451,"sku":"S0C3002-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/4e203ddd-e981-493c-ab88-b8ea3a558eaa_53772332-8934-4be9-8f73-1d6d5af8137c.jpg?v=1793080891"},{"product_id":"human-il-13-onestep-elisa-kit","title":"Human IL-13 OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu, Mk\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 3.8%；\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 4.1%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e0.3 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3.9 pg\/ml - 250 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant\t80%\t\u003cbr\u003eSerum\t90%\u003cbr\u003ePlasma\t105%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eIL-13 is a 132-amino-acid nonglycosylated cytokine that shares common features with IL-4. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 bound IL-13 R alpha 2 with high affinity and can directly promote tumor cell invasiveness and the development of tissue fibrosis. Nevertheless, like IL-4, IL-13 inhibits macrophage production of TNF, IL-1β, and pro-inflammatory chemokines, but can upregulate the synthesis of IL-12 by DCs and macrophages.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293357776971,"sku":"S0C3003-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518737555531,"sku":"S0C3003-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677700431947,"sku":"S0C3003-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/60484e1a1e704e4cbbe21b4249680b75.jpg?v=1793127635"},{"product_id":"human-igg-total-onestep-elisa-kit","title":"Human IgG (Total) OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIgG (Total)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 7.3%；\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 7.5%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e80 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e234 pg\/ml - 15000 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant\t97%\t\u003cbr\u003eSerum\t88%\u003cbr\u003ePlasma\t93%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eImmunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This kit is for the quantitative measurement of human total IgG in Human serum, plasma and supernatant from cell cultures.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293357940811,"sku":"S0C3007-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518738341963,"sku":"S0C3007-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677702594635,"sku":"S0C3007-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a33705052b3a41c4ac34dc28be2f8d2b_eaa56c71-0e65-44a5-80c8-f91a2d967e58.jpg?v=1793127746"},{"product_id":"mouse-igg-total-onestep-elisa-kit","title":"Mouse IgG (Total) OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIgG (Total)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 4.4%；\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 6.1%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eCell culture supernatant, Serum, Plasma, Cell\/Tissue Lysate\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e16 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e78 pg\/ml - 5000 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant\t104%\t\u003cbr\u003eSerum\t107%\u003cbr\u003ePlasma\t103%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eImmunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a\/ IgG2c (strain specific), IgG2b, and IgG3. The mean serum Ig levels found for 6-month-old B6 mice were 0.22 mg\/ml for IgM, 0.28 mg\/ml for IgG1, 1.22 mg\/ml for IgG2b, 0.18 mg\/ml for IgG3, 0.075 mg\/ml for IgA and about 0.7 mg\/ml for IgG2a. This kit is for the quantitative measurement of mouse total IgG in mouse serum, plasma and supernatant from cell cultures.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293357973579,"sku":"S0C3008-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518738374731,"sku":"S0C3008-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677702627403,"sku":"S0C3008-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/588a1ae4d8c5412e99ed3dc8ce6e02ed_3014ab5b-7d83-4d58-8f8c-2a0886b0e348.jpg?v=1793840502"},{"product_id":"human-il-5-onestep-elisa-kit","title":"Human IL-5 OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIL-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 4.7%；\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 6.4%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1.1 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3.9 pg\/ml - 250 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant  85%\t\u003cbr\u003eSerum  102%\u003cbr\u003ePlasma\t97%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eThe dose response curve of the International Standard (90\/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (90\/586) units, use the following equation: NIBSC (90\/586) approximate value (IU\/mL) = 0.01 x OneStep Human IL-5 value (pg\/mL).\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eIL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293358039115,"sku":"S0C3009-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518745780299,"sku":"S0C3009-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677712654411,"sku":"S0C3009-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/cff918a159bd449693ce22c8af765009.jpg?v=1793138492"},{"product_id":"human-st2-onestep-elisa-kit","title":"Human ST2 OneStep ELISA kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eST2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(74,74,74);font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size:9.0000pt\"\u003eIntra-assay: \u003c\/span\u003e\u003cspan style=\"color:rgb(74,74,74);font-size:10.5000pt\"\u003e\u003cfont\u003e4\u003c\/font\u003e%\u003cfont\u003e；\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:rgb(74,74,74);font-size:10.5000pt\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size:9.0000pt\"\u003eInter-assay: \u003c\/span\u003e\u003cspan style=\"color:rgb(74,74,74);font-size:10.5000pt\"\u003e\u003cfont\u003e4.8\u003c\/font\u003e%\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eCell culture supernatant, Serum, Plasma\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSandwich (quantitative)\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e6.8\u003c\/font\u003e pg\/ml\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e31.25\u003c\/font\u003e pg\/ml - \u003cfont\u003e2000\u003c\/font\u003e pg\/ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cspan style=\"font-size:10.5000pt\"\u003eCell culture supernatant  \u003cfont\u003e105\u003c\/font\u003e%\t\u003c\/span\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSerum  \u003cfont\u003e107\u003c\/font\u003e%\u003c\/span\u003e\u003c\/p\u003e\n\u003cspan style=\"font-size:10.5000pt\"\u003ePlasma\t9\u003cfont\u003e3\u003c\/font\u003e%\t\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003e\u003cfont\u003e6\u003c\/font\u003e0\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e minutes\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eHuman\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eThe ST2 cardiac biomarker (also known as soluble interleukin 1 receptor-like 1) is a protein biomarker of cardiac stress encoded by the IL1RL1 gene. ST2 signals the presence and severity of adverse cardiac remodeling and tissue fibrosis, which occurs in response to myocardial infarction, acute coronary syndrome, or worsening heart failure. ST2 provides prognostic information that is independent of other cardiac biomarkers such as BNP, NT-proBNP, highly sensitive troponin, GDF-15, and galectin-3. One study indicated that discrimination is independent of age, body mass index, history of heart failure, anemia and impaired kidney function or sex.sST2(Soluble ST2) is a biomarker for risk stratification in patients with heart failure (HF) and prognostic assessment. Compared with BNP and NT-proBNP, ST2 is not affected by age, factors such as BMI and renal insufficiency.Different with so many other heart markers, the level of ST2 varies rapidly with the patient's condition. This means that ST2 can help clinicians respond faster. The increase of sST2 level (\u0026gt; 35ng\/ml) was highly correlated with the severity of heart failure which can be used to predict readmission and mortality.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293358071883,"sku":"S0C3010-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518745452619,"sku":"S0C3010-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677712588875,"sku":"S0C3010-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/30886675-6b79-4a1a-a3ee-a38ba9fa6db9.png?v=1793138488"},{"product_id":"human-gm-csf-onestep-elisa-kit","title":"Human GM-CSF OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGM-CSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eIntra-assay: 4.2%；\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eInter-assay: 5.7%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant, Serum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1.8 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3.125 pg\/ml - 200 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant  88%\t\u003cbr\u003eSerum  94%\u003cbr\u003ePlasma\t95%\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCROSS REACTIVITY\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eINTERFERENCE\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eNIBSC convert\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eGM-CSF is a hematopoietic growth factor secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. GM-CSF binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and GM-CSF.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41293358137419,"sku":"S0C3011-1x96T","price":500.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":41518745845835,"sku":"S0C3011-5x96T","price":2500.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":41677712687179,"sku":"S0C3011-10x96T","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6529f184c6b64118acc46bbaad748b3f.jpg?v=1793138493"},{"product_id":"four-color-multi-label-immunofluorescence-kit","title":"Absin 4-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al. Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50ul-100ul (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( abs994 ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 99%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 88.5502%;\" colspan=\"10\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50012 (Pika) Four Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\"\u003e\u003cp\u003e\u003cstrong\u003e  Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003eTSA650\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e15mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 9.32205%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.28227%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.51133%;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.56243%;\" width=\"91\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.51347%;\" width=\"83\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.22408%;\" width=\"114\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.4861%;\" width=\"109\"\u003e\u003cp\u003e6mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 12.0782%;\" width=\"99\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.71136%;\" width=\"90\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.85892%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 650 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518128857163,"sku":"abs50012-20T","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518128889931,"sku":"abs50012-100T","price":776.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518128922699,"sku":"abs50012-50T","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/de9450a6a62443b2919419a57252c9a3.png?v=1792753430"},{"product_id":"five-color-multi-label-immunofluorescence-kit","title":"Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1. Formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2. Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3. Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4. Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5. The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6. The slice thickness is 3-5μm Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7. Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8. The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1. \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL Etc. \u003cbr\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e (1)Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e (2)Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4.\u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003eIII. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1.dewaxing and hydration \u003cbr\u003e(1) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e(2) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e(3) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e(4) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(5) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2. Microwave repair antigen \u003cbr\u003e(1) The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e(2) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e(3) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e(4) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3. Quenching and sealing \u003cbr\u003e(1) Remove that residual lotion from the slide. \u003cbr\u003e(2) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(3) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4. Primary antibody incubation \u003cbr\u003e(1) Remove the blocking fluid from the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7. A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e(3) Repeat steps 4-6\u003cbr\u003e(4) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9. The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eⅣ. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1. Quenching, transparency, sealing \u003cbr\u003e(1) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e(2) 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e(3) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, you may not do it in general) \u003cbr\u003e(4) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e(5) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2. Primary antibody incubation \u003cbr\u003e(1) Remove the closure on the slide. \u003cbr\u003e(2) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3. Secondary antibody incubation \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e(3) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e(4) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4. Fluorescence staining to amplify the signal \u003cbr\u003e(1) Remove any remaining lotion from the slide. \u003cbr\u003e(2) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e(3) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e(4) 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e(5) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e(6) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5. A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e(1) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e(2) Repeat steps 2-4 。 \u003cbr\u003e(3) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6. Nucleation and sealing \u003cbr\u003eDropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7. The stained tissue slices were observed and analyzed under a fluorescence microscope.\u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1. Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2. In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3. If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003eMultiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003eVIII. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003efixed\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eEmbedding (frozen sample)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eAntigen repair (Paraffin Section)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSodium citrate antigen retrieval solution ( 50× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003eAntibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003eAntibody eluate ( mIHC Dedicated)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003eCell permeability (Required for intracellular indicators)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eQuratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eQuenching endogenous peroxidase\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePeroxidase blocking solution ( H2O2 Method)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eclose (Serum from the same source of secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eGoat serum\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eBovine serum albumin\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003ePrimary antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eHistochemical antibody\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePrimary and secondary antibody diluents\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003eMulticolor kit (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSeven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eSix-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFive-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eFour-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTwo-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003eLung cancer Panel (containing primary antibody)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eLung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003ebuffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ）\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 96%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 79.3271%;\" colspan=\"11\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50013 (Pika) Five-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA700\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 7.94573%;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.69898%; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61693%; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.85556%; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.60942%; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%;\" width=\"85\"\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%;\" width=\"91\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%;\" width=\"92\"\u003e\u003cp\u003e8mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%;\" width=\"76\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 700 Signal amplification reaction solution, pika universal type HRP A labeled secondary antibody, an anti-fluorescence quenching sealing agent, a peroxidase blocking solution, an antibody eluate, DAPI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41938007162955,"sku":"abs50013-20T","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41938007195723,"sku":"abs50013-100T","price":1204.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41683107283019,"sku":"abs50013-50T","price":728.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c4bec0d23c8d490abe0ec540b77e61cc.png?v=1792753434"},{"product_id":"six-color-multi-labeled-immunofluorescence-staining-kit","title":"Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. 2022 Feb 9 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n5. Beauty ] Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and Doppler imaging in histological study. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWGK Germany \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample requirements \u003cbr\u003e\u003c\/strong\u003e1 , formalin-fixed wax blocks or slides, large pieces of tissue or TMA The sealing wax cannot be obviously damaged. \u003cbr\u003e2 Slide samples, tissues need to be close to slides to avoid wrinkles, slides must not be damaged, scratched or stained. \u003cbr\u003e3 Tissue minimum should contain greater than 1000 Cells. \u003cbr\u003e4 Solid tumor tissues need to be embedded in wax blocks, and necrotic tumor tissues, fine needle punctures and cell slice samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5um Left and right, use anti-fall slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive in the water bath fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove moisture, tap to remove water droplets, do not wipe the slide with paper. \u003cbr\u003e8 The glass slide is placed on 45℃ Place on a hot plate 30min (The duration of natural air-drying slides is not less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Inspection methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required Instruments and Equipment \u003c\/strong\u003e : Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, dyeing cylinder, timer, incubation wet box, cover glass, fume hood, washing bottle, fluorescence microscope, measuring cylinder 100mL measuring cylinder 1000mL  Etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol ( 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST Etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 ) Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, use signal amplification reaction solution to press 1:200 Dilute and prepare the dye working solution (use and prepare now, if the signal is weak, the proportion can be reduced to 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the preparation working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 ) Use of secondary antibodies: \u003c\/strong\u003e The kit is a universal secondary antibody for pika. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the species of the primary antibody matches before the experiment. (Kit secondary antibody is goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing Equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes shall comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e 3. Paraffin section operation steps \u003cbr\u003e\u003c\/strong\u003e1 dewaxing and hydration \u003cbr\u003e （ 1 ) Fresh xylene dip tablets 10min , repeat 3 Twice. \u003cbr\u003e （ 2 ) Gradient ethanol dip tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 ) Sterilized water washing tablets 1min , repeat 3 Twice. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 5 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 The dewaxed and hydrated glass slide is placed in a repair cup, and the antigen repair solution is used 1× Working fluid immersion. \u003cbr\u003e （ 2 ) Place the repair cup in the microwave oven and bring to a high boil. \u003cbr\u003e （ 3 ) Low Fire Maintenance 15min (Pay attention to rehydration to prevent excessive evaporation causing dry tablets). \u003cbr\u003e （ 4 ) Remove to room temperature and naturally cool to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 ) Remove that residual lotion from the slide. \u003cbr\u003e （ 2 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 3 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the blocking fluid from the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e6 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e7 A new round of dyeing (single dyeing can be directly performed on the first 8 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Elution: remove that residual washing solution on the slide, drop the antibody eluent, cover the sample area, 37℃ incubation 20min-30min The eluate was removed. (No Quenching Step Required) \u003cbr\u003e （ 3 ) Repeat steps 4-6\u003cbr\u003e （ 4 ) Multiple rounds of staining repeat steps 7 ，（ 1 ） — （ 3 ) Nucleation and sealing were performed after completion \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e9 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e 4. Operation steps of frozen sections (antibody eluate required \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, sealing \u003cbr\u003e （ 1 ) Removing the frozen glass slides from the refrigerator and restoring them to room temperature, PBST immersion 3min , repeat 3 Secondary \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde dip tablets 10-30min Sterilized water washing tablets 1min , repeat 3 Twice. (Optional, depending on sample quality) \u003cbr\u003e （ 3 ) Dropwise adding membrane breaking agent to permeate 15min ， PBST immersion 3min , repeat 3 Twice. (Optional, generally not required) \u003cbr\u003e （ 4 ) Circle the sample area on the slide with a histochemical pen, add peroxidase blocking solution dropwise to cover the sample area, and incubate 10min ， PBST immersion 3min , repeat 3 Twice. \u003cbr\u003e （ 5 ) Remove that residual washing solution on the slide, add the blocking solution dropwise, cover the sample area, and incubate at room temperature 60min The blocking solution was removed. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 ) Remove the closure on the slide. \u003cbr\u003e （ 2 ) The diluted primary antibody solution was added dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 1h (need to be optimized for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) direct dropwise addition HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 ) Moisturizing incubation at room temperature 30min 。 \u003cbr\u003e （ 4 ) with 1×PBST buffer Immersion slide 3min , repeat 3 Twice. \u003cbr\u003e4 Fluorescence staining to amplify the signal \u003cbr\u003e （ 1 ) Remove any remaining lotion from the slide. \u003cbr\u003e （ 2 ) drop-on slide with pipette 1× Dye working solution 50μL-100μL (Use signal amplifier to press 1:200 Dilute) to immerse the sample area. \u003cbr\u003e （ 3 ) Room temperature moisturizing shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Dip slides, room temperature dip slides 3min 。 repeat 3 Twice. \u003cbr\u003e （ 5 ) dropwise addition of antibody eluate ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 ) Sterilized water washing tablets 1 times, 1×PBST buffer Immersion tablet 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be directly performed on the first 6 Step) \u003cbr\u003e （ 1 ) After each round of staining, fluorescence microscope can be used to confirm the staining. PBST Cover the sample to prevent dry sheets. \u003cbr\u003e （ 2 ) Repeat steps 2-4 。 \u003cbr\u003e （ 3 ) Multiple rounds of staining repeat steps 5 Nucleation and sealing were performed after completion. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Dropwise 1×DAPI Working solution onto the sample, immerse the sample area, and incubate at room temperature 10min 。 use 1×PBST Immersion slide 3 Time, each time 2min 。 Then add antifluorescent quenching sealant dropwise, seal with cover glass, avoid bubbles, long-term storage, please use transparent nail polish to seal the edge of the cover glass. \u003cbr\u003e7 The stained tissue slices were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Description of results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigen, incubation time and temperature may lead to wrong results. \u003cbr\u003e2 In each staining process, there must be tissue positive control and reagent negative control experiments simultaneously. \u003cbr\u003e3 If the positive tissue control cannot show positive staining, the test result of this batch of samples shall be judged to be invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and detect the same tissue section 3 Tablet, the staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC ) Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e VII. Dye Information Form \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e Similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended Color Settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e Emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA Monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e 8. Other product recommendations \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Item No. \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (universal tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Antigen repair   (Paraffin Section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen retrieval solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen retrieval solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted with thermal repair, e.g. cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluate ( mIHC Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Quratone X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 Method) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Twain -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Serum from the same source of secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Primary and secondary antibody diluents \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e Multicolor kit   (containing TSA dyes, DAPI , tablet sealing agent, secondary antibody, signal amplifier) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye-enhanced version) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus ) (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (universal secondary antibody for pika and rabbit) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescence immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tricolor Multiplex Fluorescence Immunohistochemistry Kit (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Lung cancer Panel  (containing primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemical detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the help of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulticolor kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cdiv\u003eThere are complex cellular composition in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be presented in situ in tissue with the help of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and in situ protein expression. Conventional IHC detection can only display a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment, and this information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody, and HRP catalyzes the addition of fluorescein substrate in the system to produce an activated fluorescent substrate, which can be covalently bound with tyrosine on the antigen, so that fluorescein is stable and covalently bound on the sample. Thereafter, the non-covalently bound antibody was washed off by thermal repair method, and a primary antibody was replaced for a second round of incubation, and another fluorescein substrate was replaced, so that multiple labeling can be achieved by reciprocating.\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250820\/ef34141d61d1437aaa7ea11c1df1356c.jpg\" alt=\"\" width=\"694\" height=\"192\"\u003e\n\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 98%; height: 292px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 54px;\"\u003e\u003ctd style=\"width: 79.3271%; height: 54px;\" colspan=\"12\" width=\"67\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50014 (pika) six-color multiplex fluorescence immunohistochemical staining kit (pika universal secondary antibody) \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height: 76px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 76px; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003e Specifications \\ Components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 76px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 76px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 76px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e690\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 76px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003cbr\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 76px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 76px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e\u003cstrong\u003e Signal amplifier \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 76px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP Labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 76px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e\u003cstrong\u003e Peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 76px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e\u003cstrong\u003e Antibody eluate \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 76px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e\u003cstrong\u003e Anti-fluorescence quenching sealing agent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e80mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5ml×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e25mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 7.86295%; height: 54px; text-align: center;\" width=\"67\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.37447%; height: 54px; text-align: center;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.54078%; height: 54px; text-align: center;\" width=\"77\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.36551%; height: 54px; text-align: center;\" width=\"84\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29144%; text-align: center;\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 5.29147%; height: 54px; text-align: center;\" width=\"75\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.7215%; height: 54px; text-align: center;\" width=\"85\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.98258%; height: 54px; text-align: center;\" width=\"91\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.87729%; height: 54px; text-align: center;\" width=\"92\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.0496%; height: 54px; text-align: center;\" width=\"83\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.61445%; height: 54px; text-align: center;\" width=\"76\"\u003e\u003cp\u003e16mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.35509%; height: 54px; text-align: center;\" width=\"102\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA Monochromatic fluorescent dye 520 、 TSA Monochromatic fluorescent dye 570 、 TSA Monochromatic fluorescent dye 620 、 TSA Monochromatic fluorescent dye 690 、 TSA Monochromatic fluorescent dye 770 Signal amplification reaction solution, pika universal type HRP Labeled secondary antibody, anti-fluorescence quenching tablet sealing agent, DAPI Peroxidase blocking solution, antibody eluate \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is only used for immunohistochemistry, not for other purposes.\u003cbr\u003e2. This kit is limited to professionals.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of reagents with skin and eyes.\u003cbr\u003e4. The activity of reagents that have exceeded the expiration date may be reduced, so reagents that have exceeded the expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Dewaxing is not complete, which easily affects the dyeing effect.\u003cbr\u003e7. In order to prevent possible false negative and false positive results, a positive control and a negative control should be carried out simultaneously during the experiment.\u003cbr\u003e8. All kinds of wastes generated in the use of this kit should be treated in accordance with the Regulations on Medical Waste Management.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be kept away from light, stored at 2-8℃, and the validity period is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell climbing sections, and needs to be matched with abs994 antibody eluate (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePerformance Indicator\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e1. Conformity: Take the conforming tissue slices (including the positive tissue slices and the negative reagent control). After the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative.\u003cbr\u003e2. Intra-batch repeatability: Take the same batch of kits and detect 3 sections of the same tissue, and the staining results should be consistent. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41518129840203,"sku":"abs50014-20T","price":411.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41518129872971,"sku":"abs50014-100T","price":1570.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41518129905739,"sku":"abs50014-50T","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d36ad5823f9f483da4497e5d7c690fa3.png?v=1792753437"},{"product_id":"sesven-color-multi-label-immunofluorescence-kit","title":"Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. Mar 3, 2022 \n2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 \n3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications. Feb 9, 2022 \n4. Dabbs David J. Diagnostic Immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. \n5. Beauty 】Ed Harlow, David Lane. Antibody Technical Guidelines (M). Beijing: Science Press, 2002: 79-80, 105. \n6. Stack, EC, et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70(1):46-58. \n7. Qian Bangguo. Jiao Lei. Application of multi-label immunofluorescence staining and multi-population imaging techniques in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Sample Requirements \u003cbr\u003e\u003c\/strong\u003e1 Formalin-fixed wax blocks or glass slides, large tissues or TMA The sealing wax should not be obviously damaged. \u003cbr\u003e2 For slide samples, the tissue needs to be closely adhered to the slide to avoid wrinkles, and the slide should not be damaged, scratched, or stained. \u003cbr\u003e3 The organization should contain at least greater than 1000 A cell. \u003cbr\u003e4 The wax block needs to be embedded in solid tumor tissue. Necrotic tumor tissue, fine needle puncture material, and cell shedding samples will affect the staining effect. \u003cbr\u003e5 The organization should use 10% Neutral formalin fixation, normal fixation time is 18-24h 。 \u003cbr\u003e6 The slice thickness is 3-5μm Left and right, use anti-detachment slides. It is recommended that the slides be prepared within one week after fixation. \u003cbr\u003e7 Do not add any adhesive during the water bath mixing process; the sample should be placed on the front and center of the slide. Place the slides vertically on absorbent paper to remove moisture, and gently tap to remove water droplets. Do not wipe the slides with paper. \u003cbr\u003e8 The glass slide was placed on 45℃ Place on a hot plate 30min (The natural air-drying time for glass slides shall not be less than 1h ）。 \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e II. Testing Methods \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003cstrong\u003e1 、 \u003c\/strong\u003e\u003cstrong\u003e Required instruments and equipment \u003c\/strong\u003e Pipette, constant temperature drying oven, microwave oven, immunohistochemistry pen, repair cup, staining vat, timer, incubation wet box, cover glass, fume hood, bottle washer, fluorescence microscope, measuring cylinder 100mL ,measuring cylinder 1000mL  etc. \u003cbr\u003e\u003cstrong\u003e2 、 \u003c\/strong\u003e\u003cstrong\u003e Reagents required: \u003c\/strong\u003e Sterilized deionized water ( \u003cstrong\u003eabs9259\u003c\/strong\u003e ), xylene, ethanol 100% 、 95% 、 70% ）、 10% Neutral formalin, antigen repair primary antibody, blocking solution, PBST etc. \u003cbr\u003e\u003cstrong\u003e3 、 \u003c\/strong\u003e\u003cstrong\u003e Reagent Preparation: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e （ 1 Dilution of fluorescent dyes: \u003c\/strong\u003e The dye is 200× Mother liquor, using signal amplification reaction solution according to 1:200 Dilute and prepare the dye working solution (prepare on the spot; if the signal is weak, reduce the ratio to...). 1:100 ）； DAPI Use sterile water to press 1:100 Dilute the prepared working solution. \u003cbr\u003e\u003cstrong\u003e （ 2 Use of secondary antibodies: \u003c\/strong\u003e The kit is a general pika secondary antibody. It is not recommended that the sample be homologous to the secondary antibody. Please verify whether the primary antibody species matches before the experiment. (The second antibody in the kit is a goat species) \u003cbr\u003e\u003cstrong\u003e4 、 \u003c\/strong\u003e\u003cstrong\u003e Testing equipment: \u003c\/strong\u003e A fluorescence microscope or a fluorescence full-film scanning device, TSA Excitation and emission filters suitable for monochromatic fluorescent dyes should comply with the recommendations in the table. \u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e III. Paraffin sectioning operation steps \u003cbr\u003e\u003c\/strong\u003e1 Dewaxing hydration \u003cbr\u003e （ 1 Fresh xylene-soaked tablets 10min , repeat 3 Next. \u003cbr\u003e （ 2 Gradient ethanol-soaked tablets: 100% 5min ； 95% 5min ； 70% 2min 。 \u003cbr\u003e （ 3 Sterilized water-washed tablets 1min , repeat 3 Next. \u003cbr\u003e （ 4 ） 10% Neutral formalin or 4% paraformaldehyde impregnated tablet 10-30min Sterilized water-washed tablets 1min , repeat 3 Next. (Optional, depending on sample quality) \u003cbr\u003e （ 5 Add drops of membrane-breaking agent to permeate the membrane. 15min ， PBST immersion 3min , repeat 3 Next. (Optional, generally not required) \u003cbr\u003e2 Microwave repair antigen \u003cbr\u003e （ 1 Place the dewaxed and hydrated glass slides in a repair cup and use the antigen repair solution. 1× Working fluid immersion. \u003cbr\u003e （ 2 Place the repair cup in a microwave oven and bring to a boil over high heat. \u003cbr\u003e （ 3 Maintain low heat. 15min (Pay attention to rehydration to prevent excessive evaporation from causing dry tablets). \u003cbr\u003e （ 4 Remove from room temperature and cool naturally to room temperature. \u003cbr\u003e3 Quenching and sealing \u003cbr\u003e （ 1 Remove any remaining washing solution from the slide. \u003cbr\u003e （ 2 Circle the sample area on the slide with a histochemical pen, add peroxidase sealing solution dropwise to cover the sample area, and incubate. 10min ， PBST immersion 3min , repeat 3 Next. \u003cbr\u003e （ 3 Remove any remaining washing solution from the slide, add sealing solution dropwise, cover the sample area, and incubate at room temperature. 60min , removing the blocking solution. \u003cbr\u003e4 Primary antibody incubation \u003cbr\u003e （ 1 Remove the sealing solution from the slide. \u003cbr\u003e （ 2 Add the diluted primary antibody solution dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 Room temperature humidification and shaking incubation 1h (Requires optimization and adjustment for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) use 1×PBST buffer immersion glass slide 3min , repeat 3 Next. \u003cbr\u003e5 Secondary antibody incubation \u003cbr\u003e （ 1 Remove any remaining washing solution from the slide. \u003cbr\u003e （ 2 Add directly dropwise HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 Room temperature humidification incubation 30min 。 \u003cbr\u003e （ 4 ) use 1×PBST buffer immersion glass slide 3min , repeat 3 Next. \u003cbr\u003e6 Fluorescence staining amplification signal \u003cbr\u003e （ 1 Remove any remaining washing solution from the slide. \u003cbr\u003e （ 2 Add dropwise onto the slide using a pipette. 1× dye working fluid 50μL-100μL (Use signal amplification solution according to 1:200 Dilution) Submerge the sample area. \u003cbr\u003e （ 3 Room temperature humidification and shaking incubation 10-15min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Immersion glass slides, room temperature immersion slides 3min 。 Repeat 3 Next. \u003cbr\u003e7 A new round of dyeing (single dyeing can be performed directly). 8 step) \u003cbr\u003e （ 1 After each round of staining, the staining status can be confirmed using a fluorescence microscope. Pay attention to... PBST Cover the sample to prevent dry pieces. \u003cbr\u003e （ 2 Elution: Remove residual washing solution from the slide, add antibody eluent dropwise, and cover the sample area. 37℃ incubation 20min-30min , removing the eluent. (No quenching step required) \u003cbr\u003e （ 3 Repeat the steps. 4-6\u003cbr\u003e （ 4 Multiple rounds of staining are repeated. 7 ，（ 1 ） — （ 3 After completion, nucleation and sealing were performed. \u003cbr\u003e8 Nucleation and sealing \u003cbr\u003e Drip 1×DAPI Apply working solution to sample, immerse sample area, and incubate at room temperature 10min 。 use 1×PBST immersion glass slide 3 Times, every time 2min 。 Then add anti-fluorescence quenching sealing agent dropwise, seal the tablet with a coverslip to avoid bubbles, and seal the edge of the coverslip with clear nail polish for long-term storage. \u003cbr\u003e9 The stained tissue sections were observed and analyzed under a fluorescence microscope. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e IV. Frozen section operation steps (need to be combined with antibody eluent \u003cspan style=\"background-color: #f1c40f;\"\u003eabs994\u003c\/span\u003e ） \u003cbr\u003e\u003c\/strong\u003e1 Quenching, transparency, and sealing \u003cbr\u003e （ 1 Remove the frozen slides from the refrigerator and allow them to return to room temperature. PBST immersion 3min , repeat 3 Next \u003cbr\u003e （ 2 ） 10% Neutral formalin or 4% paraformaldehyde impregnated tablet 10-30min Sterilized water-washed tablets 1min , repeat 3 Next. (Optional, depending on sample quality) \u003cbr\u003e （ 3 Add drops of membrane-breaking agent to permeate the membrane. 15min ， PBST immersion 3min , repeat 3 Next. (Optional, generally not required) \u003cbr\u003e （ 4 Circle the sample area on the slide with a histochemical pen, add peroxidase sealing solution dropwise to cover the sample area, and incubate. 10min ， PBST immersion 3min , repeat 3 Next. \u003cbr\u003e （ 5 Remove any remaining washing solution from the slide, add sealing solution dropwise, cover the sample area, and incubate at room temperature. 60min , removing the blocking solution. \u003cbr\u003e2 Primary antibody incubation \u003cbr\u003e （ 1 Remove the seal from the slide. \u003cbr\u003e （ 2 Add the diluted primary antibody solution dropwise with a pipette to immerse the sample area. \u003cbr\u003e （ 3 Room temperature humidification and shaking incubation 1h (Requires optimization and adjustment for different antibodies) or 4℃ Incubate overnight. \u003cbr\u003e （ 4 ) use 1×PBST buffer immersion glass slide 3min , repeat 3 Next. \u003cbr\u003e3 Secondary antibody incubation \u003cbr\u003e （ 1 Remove any remaining washing solution from the slide. \u003cbr\u003e （ 2 Add directly dropwise HRP Secondary antibody working solution, immerse the sample area. \u003cbr\u003e （ 3 Room temperature humidification incubation 30min 。 \u003cbr\u003e （ 4 ) use 1×PBST buffer immersion glass slide 3min , repeat 3 Next. \u003cbr\u003e4 Fluorescence staining amplification signal \u003cbr\u003e （ 1 Remove any remaining washing solution from the slide. \u003cbr\u003e （ 2 Add dropwise onto the slide using a pipette. 1× dye working fluid 50μL-100μL (Use signal amplification solution according to 1:200 Dilution) Submerge the sample area. \u003cbr\u003e （ 3 Room temperature humidification and shaking incubation 10min 。 \u003cbr\u003e （ 4 ） 1×PBST buffer Immersion glass slides, room temperature immersion slides 3min 。 Repeat 3 Next. \u003cbr\u003e （ 5 Add antibody eluent dropwise ( \u003cstrong\u003eabs994\u003c\/strong\u003e ）， 37℃ incubation 15-20min 。 \u003cbr\u003e （ 6 Sterilized water-washed tablets 1 next time, 1×PBST buffer immersion tablets 2min 。 \u003cbr\u003e5 A new round of dyeing (single dyeing can be performed directly). 6 step) \u003cbr\u003e （ 1 After each round of staining, the staining status can be confirmed using a fluorescence microscope. Pay attention to... PBST Cover the sample to prevent dry pieces. \u003cbr\u003e （ 2 Repeat the steps. 2-4 。 \u003cbr\u003e （ 3 Multiple rounds of staining are repeated. 5 After completion, nucleation and sealing were performed. \u003cbr\u003e6 Nucleation and sealing \u003cbr\u003e Drip 1×DAPI Apply working solution to sample, immerse sample area, and incubate at room temperature 10min 。 use 1×PBST immersion glass slide 3 Times, every time 2min 。 Then add anti-fluorescence quenching sealing agent dropwise, seal the tablet with a coverslip to avoid bubbles, and seal the edge of the coverslip with clear nail polish for long-term storage. \u003cbr\u003e7 The stained tissue sections were observed and analyzed under a fluorescence microscope. \u003cp\u003e\u003cstrong\u003e V. Explanation of Results: \u003cbr\u003e\u003c\/strong\u003e1 Changes in microwave repair antigens, incubation time, and temperature may yield erroneous results. \u003cbr\u003e2 During each staining process, positive tissue control and negative reagent control experiments must be performed simultaneously. \u003cbr\u003e3 If the positive tissue control does not show positive staining, the test result of this batch of samples should be deemed invalid. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Product performance indicators: \u003cbr\u003e\u003c\/strong\u003e1 Compliance: Take compliant tissue pieces (including positive tissue pieces and negative reagent controls), and after the corresponding immunohistochemical test, the staining result of the positive control is positive, and the staining result of the negative control is negative. \u003cbr\u003e2 Intra-batch repeatability: Take the same batch of kits and test the same tissue section. 3 The staining results should be consistent. \u003cbr\u003e Multiplex fluorescence immunohistochemistry ( mIHC Experimental operation demonstration video \u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cstrong\u003e 7. Dye Information Table \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; height: 263px; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%; text-align: center;\" rowspan=\"2\" width=\"159\"\u003e\u003cstrong\u003e Dye Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%; text-align: center;\" colspan=\"2\" width=\"162\"\u003e\u003cstrong\u003e wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%; text-align: center;\" rowspan=\"2\" width=\"183\"\u003e\u003cstrong\u003e similar fluorescent dyes \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px; text-align: center;\" rowspan=\"2\"\u003e\u003cstrong\u003e Recommended color settings \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e excitation wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e\u003cstrong\u003e emission wavelength \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #19843b;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF594\/Opal620\/TexasRed\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY5\/AF610\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF647\/CY5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eAF680\/CY5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%; text-align: center;\" width=\"159\"\u003eTSA monochromatic fluorescent dye 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%; text-align: center;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%; text-align: center;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%; text-align: center;\" width=\"183\"\u003eCY7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px; text-align: center;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e                \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003e\u003cbr\u003e VIII. Other Product \u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto; height: 837px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e\u003cstrong\u003e classification \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003e\u003cstrong\u003e Product number \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e fixed \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e4% Paraformaldehyde (general-purpose tissue fixation solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e Embedding (frozen sample) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eOCT embedding agent \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e110mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e antigen repair   (Paraffin section) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003eTris-EDTA Antigen repair solution ( 10× ， pH9.0 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Sodium citrate antigen repair solution ( 50× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 67px;\"\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"129\"\u003e Antibody elution (samples that cannot be eluted using thermal repair, such as cells, frozen, bone tissue, etc.) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"316\"\u003e Antibody eluent ( mIHC (Dedicated) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 67px;\" width=\"90\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"129\"\u003e cell permeability   (Required for intracellular indicators) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tralaton X-100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e quenching endogenous peroxidase \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Peroxidase blocking solution ( H2O2 law) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Tween -20\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\/2.5L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e close   (Secondary antibody and serum of the same origin) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e goat serum \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e bovine serum albumin \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e Primary antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e histochemical antibody \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e primary antibody and secondary antibody dilution \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 396px;\" rowspan=\"18\" width=\"129\"\u003e multicolor reagent kit   (contains TSA dyes, DAPI (Tablet sealant, secondary antibody, signal amplification solution) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye Enhanced Edition) (Anti-Rabbit Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( 770 Dye Enhanced Edition) (Pika Universal Secondary Antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescent immunohistochemical staining kit (pika general secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus (Pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescent immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Seven-color multiplex fluorescence immunohistochemical staining kit ( plus (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescent immunohistochemical staining kit (pika general secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus (Pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescent immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Six-color multiplex fluorescence immunohistochemical staining kit ( plus (Anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescent immunohistochemical staining kit (pika general secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Five-color multiplex fluorescent immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescent immunohistochemical staining kit (pika general secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Four-color multiplex fluorescent immunohistochemical staining kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\/50T\/100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Three-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Three-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (pika universal secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Two-color multiplex fluorescence immunohistochemistry kit (anti-rabbit secondary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e100T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 44px;\" rowspan=\"2\" width=\"129\"\u003e lung cancer Panel  (Including primary antibody) \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemistry detection kit ( I ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003e Lung cancer tumor microenvironment multiplex fluorescence immunohistochemistry detection kit ( II ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e20T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 66px;\" rowspan=\"3\" width=\"129\"\u003e Buffer \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9341\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST(10×,pH7.4)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBST （ 1× ， pH7.4 ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"316\"\u003ePBS Buffer ( 1× ） \u003c\/td\u003e\n\u003ctd style=\"text-align: center; height: 22px;\" width=\"90\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescent immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Secondary antibodies labeled with horseradish peroxidase (HRP) are selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved through multiple staining cycles by means of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003emulticolor reagent kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe tissue microenvironment contains complex cellular composition, and the phenotype, state, abundance, and distribution of these cells have important biological significance and clinical value. This can be represented in situ in the tissue by means of antibody staining. Immunohistochemical staining is a common technique for studying tissue morphology and in situ protein expression. Conventional IHC detection can only show a single indicator and is difficult to present the composition, state and relationship of cells in the complex tissue microenvironment. This information is crucial for the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibodies. HRP catalyzes the addition of fluorescein substrates to the system to produce activated fluorescein substrates. The activated substrates can covalently bind to tyrosine on the antigen, so that fluorescein is stably covalently bound to the sample. After that, the non-covalently bound antibody is washed away by heat repair, and the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced. In this way, multiple labeling can be achieved.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"border-collapse: collapse; width: 95%; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\u003ctd style=\"width: 78.9156%;\" colspan=\"13\" width=\"1064\"\u003e\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eabs50015 (Pika) Seven-Color Multiplex Fluorescence Immunohistochemical Staining Kit \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e\u003cstrong\u003e Specification \\ components \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e480\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e520\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e570\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e620\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e690\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eTSA\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e770\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e\u003cstrong\u003eDAPI 100x\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e\u003cstrong\u003e signal amplifying liquid \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e\u003cstrong\u003e polymerization HRP labeled secondary antibody ( Goat Source )\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e\u003cstrong\u003e peroxidase blocking solution \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e\u003cstrong\u003e antibody eluent \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e\u003cstrong\u003e anti-fluorescence quenching tablet encapsulant \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e100T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e50μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e100mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL×2\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e50T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e25μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e30mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e10mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e50mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 8.03933%;\" width=\"68\"\u003e\u003cp\u003e20T\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.18135%;\" width=\"82\"\u003e\u003cp\u003e10μLl\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 3.95428%;\" width=\"82\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.57206%;\" width=\"78\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.00121%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.86194%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.92212%;\" width=\"71\"\u003e\u003cp\u003e10μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 4.39566%;\" width=\"71\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.05063%;\" width=\"85\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10.436%;\" width=\"86\"\u003e\u003cp\u003e12mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.46907%;\" width=\"70\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 6.09118%;\" width=\"78\"\u003e\u003cp\u003e20mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 8.94076%;\" width=\"74\"\u003e\u003cp\u003e5mL\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003eTSA monochromatic fluorescent dye 480 、 TSA monochromatic fluorescent dye 520 、 TSA monochromatic fluorescent dye 570 、 TSA monochromatic fluorescent dye 620 、 TSA monochromatic fluorescent dye 690 、 TSA monochromatic fluorescent dye 770 Signal amplification reaction solution, pika general type HRP Labeled secondary antibodies, anti-fluorescence quenching tablet sealing agents, DAPI Peroxidase blocking solution, antibody eluent \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit is for immunohistochemistry only and will not be used for other purposes.\u003cbr\u003e2. This kit is for professional use only.\u003cbr\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with skin and eyes.\u003cbr\u003e4. Reagents exceeding their expiration date may have reduced activity, so reagents exceeding their expiration date should not be used.\u003cbr\u003e5. If the dyeing components of this kit are mixed with products from other companies, abnormalities may occur during the dyeing process.\u003cbr\u003e6. Incomplete dewaxing can easily affect the dyeing effect.\u003cbr\u003e7. To prevent possible false negative or false positive results, both positive and negative controls must be conducted simultaneously during the experiment.\u003cbr\u003e8. All waste generated from this kit should be treated in accordance with the \"Regulations on the Management of Medical Waste\".\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored away from light at 2~8℃ and have a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell clipping, and needs to be combined with abs994 antibody eluent (mIHC specific).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683107315787,"sku":"abs50015-20T","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41521814962251,"sku":"abs50015-100T","price":2046.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":41521814995019,"sku":"abs50015-50T","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d4b127f4e0554a5daba97bbb65ce9238.png?v=1794218483"},{"product_id":"tsa480","title":"mIHC Dye (TSA480)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 480, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518141571147,"sku":"abs50075-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa520","title":"mIHC Dye (TSA520)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eI. Sample Requirements\u003cbr\u003e\u003c\/strong\u003e 1. Formalin-fixed paraffin blocks or slides; large tissue blocks or TMAs; paraffin seal must be intact.\u003cbr\u003e2. For slide samples, tissue must adhere tightly to the slide without folds; slides must be free of cracks, scratches or stains.\u003cbr\u003e3. Tissue must contain \u0026gt;1,000 cells.\u003cbr\u003e4. Only solid-tumor tissue should be embedded; necrotic tumor, fine-needle aspirates or cytospins will impair staining.\u003cbr\u003e5. Tissue must be fixed in 10 % neutral buffered formalin for 18–24 h.\u003cbr\u003e6. Cut 4-µm sections on adhesion slides; prepare slides within one week after fixation.\u003cbr\u003e7. Do NOT add any adhesives to the water bath; place tissue on the front centre of the slide. Remove water by standing the slide vertically on absorbent paper; tap off drops—never wipe.\u003cbr\u003e8. Dry slide on a 45 °C hot-plate for 30 min (air-dry ≥1 h if no hot-plate).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eII. Assay Procedure\u003cbr\u003e\u003c\/strong\u003e \u003cstrong\u003e1. Equipment required:\u003c\/strong\u003e pipettes, oven, microwave, IHC pen, retrieval jar, staining jars, timer, humid chamber, cover-slips, fume hood, wash bottle, fluorescence microscope, 100 mL \u0026amp; 1,000 mL cylinders, etc.\u003cbr\u003e\u003cstrong\u003e2. Reagents required:\u003c\/strong\u003e sterile water (abs9259), xylene, ethanol (100 %, 95 %, 70 %), 10 % neutral buffered formalin, antigen-retrieval buffer, primary antibodies, polymer HRP-secondary antibodies (abs20216, abs50213), blocking buffer, TBST, anti-fade mounting medium.\u003cbr\u003e\u003cstrong\u003e3. Reagent preparation:\u003cbr\u003e\u003c\/strong\u003e   1) Fluorophore dilution: 100× stock dyes are diluted 1:100 in amplification buffer (prepare fresh).\u003cbr\u003e\u003cstrong\u003e4. Imaging:\u003c\/strong\u003e fluorescence microscope or slide scanner; filter sets must match dye specifications below.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIII. Paraffin-Section Protocol\u003cbr\u003e\u003c\/strong\u003e 1. De-paraffinisation \u0026amp; re-hydration\u003cbr\u003ea) Fresh xylene 10 min ×3.\u003cbr\u003eb) Ethanol gradient: 100 % 5 min → 95 % 5 min → 70 % 2 min.\u003cbr\u003ec) Sterile water 1 min ×3.\u003cbr\u003ed) Optional: 10 % neutral formalin or 4 % PFA 10–30 min, then water 1 min ×3.\u003cbr\u003ee) Optional: permeabilisation 15 min, PBST 3 min ×3.\u003cbr\u003e2. Antigen retrieval (microwave)\u003cbr\u003ea) Place slides in retrieval buffer (1×) in microwave jar.\u003cbr\u003eb) High power to boil; maintain low boil 15 min (top up liquid).\u003cbr\u003ec) Cool to RT.\u003cbr\u003e3. Quench \u0026amp; block\u003cbr\u003ea) Remove liquid; circle tissue with IHC pen.\u003cbr\u003eb) 3 % H₂O₂ 10 min; PBST 3 min ×3.\u003cbr\u003ec) Blocking buffer 10–30 min RT with shaking; discard.\u003cbr\u003e4. Primary antibody\u003cbr\u003ea) Add diluted primary Ab to cover tissue.\u003cbr\u003eb) Incubate 1 h RT with shaking; optimise if needed.\u003cbr\u003ec) TBST 3 min ×2.\u003cbr\u003e5. Secondary antibody\u003cbr\u003ea) Polymer-HRP secondary (abs20216 or abs50213) 10 min RT.\u003cbr\u003eb) TBST 3 min ×2.\u003cbr\u003e6. Fluorescent TSA amplification\u003cbr\u003ea) Apply 100 µL 1× dye working solution 10 min RT with shaking.\u003cbr\u003eb) TBST 3 min ×3.\u003cbr\u003ec) Microwave retrieval; cool to RT.\u003cbr\u003ed) Sterile water 1 min → TBST 2 min.\u003cbr\u003e7. Next cycle (skip for single stain)\u003cbr\u003ea) Check signal under microscope (keep tissue wet).\u003cbr\u003eb) Block 10–30 min (no H₂O₂ quench).\u003cbr\u003ec) Repeat steps 4–6.\u003cbr\u003ed) After final cycle proceed to nuclear staining.\u003cbr\u003e8. Nuclear stain \u0026amp; mounting\u003cbr\u003ea) 1× DAPI 5 min RT.\u003cbr\u003eb) TBST 2 min ×3.\u003cbr\u003ec) Add anti-fade medium; cover-slip, avoid bubbles; seal edges with clear nail polish for long-term storage.\u003cbr\u003e9. Image \u0026amp; analyse.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIV. Frozen-Section Protocol (requires antibody-elution buffer abs994)\u003cbr\u003e\u003c\/strong\u003e 1. Quench, permeabilise, block\u003cbr\u003ea) Equilibrate slide to RT; PBST 3 min ×3.\u003cbr\u003eb) Optional: 10 % neutral formalin or 4 % PFA 10–30 min; water 1 min ×3.\u003cbr\u003ec) Optional: permeabilisation 15 min; PBST 3 min ×3.\u003cbr\u003ed) 3 % H₂O₂ 10 min; PBST 3 min ×3.\u003cbr\u003ee) Blocking buffer 10–30 min RT; discard.\u003cbr\u003e2. Primary antibody – same as paraffin step 4.\u003cbr\u003e3. Secondary antibody – same as paraffin step 5.\u003cbr\u003e4. TSA amplification\u003cbr\u003ea) 1× dye working solution 10 min RT.\u003cbr\u003eb) TBST 3 min ×3.\u003cbr\u003ec) Antibody-elution buffer (abs994) 15–20 min 37 °C.\u003cbr\u003ed) Sterile water 1 min → TBST 2 min.\u003cbr\u003e5. Next cycle (skip for single stain)\u003cbr\u003ea) Check signal; keep wet.\u003cbr\u003eb) Block 10–30 min.\u003cbr\u003ec) Repeat steps 2–4.\u003cbr\u003e6. Nuclear stain \u0026amp; mounting – identical to paraffin step 8.\u003cbr\u003e7. Image \u0026amp; analyse.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eV. Result Interpretation\u003cbr\u003e\u003c\/strong\u003e 1. Any change in retrieval, incubation time or temperature may invalidate results.\u003cbr\u003e2. Each run must include positive tissue control and negative reagent control.\u003cbr\u003e3. If positive control fails, the entire batch is invalid.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eVI. Performance Characteristics\u003cbr\u003e\u003c\/strong\u003e 1. Accuracy: positive control tissue shows expected staining; negative control is blank.\u003cbr\u003e2. Within-batch precision: triplicate sections stained with the same kit lot must yield identical patterns.\u003cbr\u003eMultiplex fluorescent IHC demonstration video\u003cbr\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250818\/8e8ccd6ef85b4e6dbda04ed662561a9f.jpg\" alt=\"\" width=\"101\" height=\"100\"\u003e\u003cbr\u003e\u003cstrong\u003eVII. Dye Spectral Data\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 43px; width: 23.7159%;\" rowspan=\"2\" width=\"159\"\u003eDye\u003c\/td\u003e\n\u003ctd style=\"height: 21px; width: 28.1679%;\" colspan=\"2\" width=\"162\"\u003eWavelength (nm)\u003c\/td\u003e\n\u003ctd style=\"height: 43px; width: 26.6196%;\" rowspan=\"2\" width=\"183\"\u003eAnalogues\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 43px;\" rowspan=\"2\"\u003eDisplay Colour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003eEx\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003eEm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e360\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e461\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eDAPI\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #272dd1;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e450\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e480\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eOpal480\/Aqua\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #0c5c0d;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e490\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eFITC\/AF488\/Opal520\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #2dc26b;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e520\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e540\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF514\/Opal540\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f2f930;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e550\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e570\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy3\/AF555\/Opal570\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #f1c40f;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e590\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e620\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF594\/Opal620\/Texas-Red\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #df534b;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e630\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e650\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy5\/AF647\/Opal650\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #a30707;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 690\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e640\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e670\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF647\/Cy5\/Opal670\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #7e0036;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 700\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e680\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e702\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eAF680\/Cy5.5\/Opal690\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #843fa1;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px; width: 23.7159%;\" width=\"159\"\u003eTSA 770\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 14.4489%;\" width=\"81\"\u003e740\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 13.719%;\" width=\"81\"\u003e780\u003c\/td\u003e\n\u003ctd style=\"height: 22px; width: 26.6196%;\" width=\"183\"\u003eCy7\/Opal780\u003c\/td\u003e\n\u003ctd style=\"width: 17.0494%; height: 22px;\"\u003e\u003cspan style=\"background-color: #ced4d9;\"\u003e          \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"text-align: justify;\"\u003e\u003cstrong\u003eVIII. Related Products\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; margin-left: auto; margin-right: auto; height: 881px;\" border=\"1\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003e\u003cstrong\u003eCategory\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"80\"\u003e\u003cstrong\u003eCat. No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eFixation\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9179\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4 % Paraformaldehyde (universal fixative)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eEmbedding (frozen)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9756\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eOCT Compound\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e110 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eAntigen retrieval (paraffin)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9342\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTris-EDTA retrieval buffer (10×, pH 9.0)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9248\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eCitrate retrieval buffer (50×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003eAntibody elution (cells, frozen, bone)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs994\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eAntibody Elution Buffer (mIHC grade)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"129\"\u003ePermeabilisation\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9149\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTriton X-100\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eEndogenous peroxidase quench\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs9333\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePeroxidase Block (H₂O₂ method)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9152\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTween-20\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL \/ 2.5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eBlocking (serum)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs933\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eGoat Serum\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9157\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eBSA\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003ePrimary antibodies\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs1\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eIHC-validated antibodies\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9299\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eAb diluent\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"18\" width=\"129\"\u003eMultiplex kits (TSA dyes, DAPI, mount, secondary, buffer)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50165\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (770 enhanced, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50166\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (770 enhanced, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50015\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50037\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (plus, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50031\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50038\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e7-Color mIHC Kit (plus, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50014\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50049\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (plus, universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50030\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50048\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e6-Color mIHC Kit (plus, anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50013\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e5-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50029\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e5-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50012\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50028\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e4-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50089\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e3-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50088\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e3-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50087\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e2-Color mIHC Kit (universal)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50086\u003c\/td\u003e\n\u003ctd width=\"316\"\u003e2-Color mIHC Kit (anti-rabbit)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003eLung-cancer panels (include primaries)\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs50083\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eLung TME mIHC Panel I\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50084\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eLung TME mIHC Panel II\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e20 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"3\" width=\"129\"\u003eWash buffers\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs952\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eTBST (10×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL ×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs9340\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBST (1×, pH 7.4)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs962\u003c\/td\u003e\n\u003ctd width=\"316\"\u003ePBS (1×)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"2\" width=\"129\"\u003ePolymer-HRP secondary\u003c\/td\u003e\n\u003ctd width=\"80\"\u003eabs20216\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eHigh-sensitivity Poly-HRP Goat anti-Rabbit IgG (H+L)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd width=\"80\"\u003eabs50213\u003c\/td\u003e\n\u003ctd width=\"316\"\u003eHigh-sensitivity Poly-HRP Goat anti-Rabbit\/Mouse IgG (H+L)\u003c\/td\u003e\n\u003ctd width=\"90\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 520, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518141603915,"sku":"abs50076-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa570","title":"mIHC Dye (TSA570)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Antibody Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003c\/p\u003e\n\u003cp\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 570, signal amplification reaction solution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This kit is only used for immunohistochemistry and is not used for other purposes.\u003c\/p\u003e\n\u003cp\u003e2. This kit is restricted to professional use only.\u003c\/p\u003e\n\u003cp\u003e3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes.\u003c\/p\u003e\n\u003cp\u003e4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used.\u003c\/p\u003e\n\u003cp\u003e5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process.\u003c\/p\u003e\n\u003cp\u003e6. Incomplete dewaxing will easily affect the dyeing effect.\u003c\/p\u003e\n\u003cp\u003e7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment.\u003c\/p\u003e\n\u003cp\u003e8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142226507,"sku":"abs50077-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa620","title":"mIHC Dye (TSA620)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 620, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142259275,"sku":"abs50078-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa700","title":"mIHC Dye (TSA700)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTSA monochromatic fluorescent dye 700, signal amplification reaction solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518142292043,"sku":"abs50079-100T","price":498.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tsa770","title":"mIHC Dye (TSA770) Two-step Method","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. \n2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. \nStack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. \n4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. \n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMultiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases!\u003cbr\u003ePrinciple of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eTSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged.\u003cbr\u003e2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained.\u003cbr\u003e3. The tissue should contain at least more than 1000 cells.\u003cbr\u003e4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect.\u003cbr\u003e5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h.\u003cbr\u003e6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation.\u003cbr\u003e7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper.\u003cbr\u003e8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41518143144011,"sku":"abs50080-100T","price":548.0,"currency_code":"USD","in_stock":true}]},{"product_id":"lung-cancer-like-organ-culture-medium-kit","title":"Human Lung Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original :\u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human lung cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human lung cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human lung cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human lung cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Kit Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 157px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 10px; text-align: center;\"\u003e human lung cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 10px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e digestive fluid of primary tissue of human lung cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e organoid primary digestive juice  D\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 39.8963%; height: 21px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; height: 21px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8963%; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 20.1721%; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199930955,"sku":"abs9443-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92.jpg?v=1792922438"},{"product_id":"colorectal-cancer-organ-culture-medium-kit","title":"Human Colorectal cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human colorectal cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human colorectal cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human colorectal cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  human colorectal cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 80%; height: 154px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e human colorectal cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e Digestive fluid of primary tissue of human colorectal cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 21px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 21px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 18px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 18px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 41.7472%; height: 10px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 20.8478%; height: 10px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199963723,"sku":"abs9445-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e413b8087fd94ae896ce7306dedca286.png?v=1792926023"},{"product_id":"breast-cancer-like-organ-culture-medium-kit","title":"Human Breast Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human breast cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human breast cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  human breast cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  human breast cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Product Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human breast cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of human breast cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518199996491,"sku":"abs9446-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8b75981794ca4051af93267e3e17a4d2.jpg?v=1792926025"},{"product_id":"pancreatic-cancer-organ-like-culture-medium-kit","title":"Human Pancreatic Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human pancreatic cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human pancreatic cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human pancreatic cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human pancreatic cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human pancreatic cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human pancreatic cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200029259,"sku":"abs9447-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_f0428346-ce8f-43a2-aadd-6fee81f35665.jpg?v=1792926026"},{"product_id":"endometrial-cancer-like-organ-culture-medium-kit","title":"Human Endometrial carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human endometrial cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human endometrial carcinoma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human endometrial carcinoma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human endometrial carcinoma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human endometrial carcinoma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human endometrial carcinoma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200062027,"sku":"abs9448-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_dba3023f-ff8f-4d43-ab09-e83833c454f4.jpg?v=1792926029"},{"product_id":"gastric-cancer-like-organ-culture-medium-kit","title":"Human Gastric Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissues of human gastric cancer for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of human gastric cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human gastric cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human gastric cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human gastric cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human gastric cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518200094795,"sku":"abs9449-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_9ad35af4-1c55-41a6-8b4f-c7bb00e6055e.jpg?v=1792926033"},{"product_id":"mouse-intestinal-organ-culture-medium-reagent","title":"Mouse Intestinal Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The normal small intestine tissue of mice after sampling must be placed in a precooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and stem cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities. Use ophthalmic scissors or a scalpel to remove the intestinal mucosa of the small intestine tissue and cut it into a volume of approximately...  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissue of normal small intestine in mice for tissue use  C  digestion, 4℃ oscillation digestion 10min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal small intestine organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organ collection, matrix gel was added for resuspension, and each well... 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL  mouse normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL  mouse normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Small Intestine Organoid Media Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e Kit Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 164px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e mouse normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e Digestive fluid of primary tissue of normal small intestine in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 61.6715%; height: 10px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 34.6761%; height: 10px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518201700427,"sku":"abs9514-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_6cbe8cbb-759e-48a4-89d2-44592c99a623.jpg?v=1792926058"},{"product_id":"mouse-lung-organ-culture-medium-kit","title":"Mouse Lung Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary lung tissue in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL Mouse normal lung organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Mouse normal lung organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Lung Organoid Culture Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Mouse normal lung organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal mouse lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202257483,"sku":"abs9515-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_a273e994-1e29-418e-bc24-2bc38bb712f0.jpg?v=1792926060"},{"product_id":"mouse-liver-like-organ-culture-medium-kit","title":"Mouse Liver Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After cleaning three times, remove impurities, and use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal primary liver tissue in mice for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25uL  Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse normal liver organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25uL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse normal liver organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500uL mouse normal liver organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOrganotial Mouse Liver Organoid Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 72.89%; height: 176px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e mouse normal liver organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e Digestive fluid of normal liver primary tissue in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50.2098%; height: 22px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 25.7015%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202290251,"sku":"abs9516-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ebe1f9c0-9bfb-44f6-93d5-d1c2b94a79e8.jpg?v=1792926063"},{"product_id":"ovarian-cancer-organoid-culture-medium-kit","title":"Human Ovarian Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Human ovarian cancer primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human ovarian cancer organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human ovarian cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human ovarian cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human ovarian cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human ovarian cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202486859,"sku":"abs9558-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_7d4d4c02-5c53-4196-8bb2-6ae8a2943306.jpg?v=1792926088"},{"product_id":"pig-endometrial-like-organ-culture-medium-kit","title":"Pig Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Pig endometrial primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of porcine endometrial organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul porcine endometrial organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul porcine endometrial organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e porcine endometrial organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Porcine endometrial primary tissue digestive fluid  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202552395,"sku":"abs9583-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_35b3c2d1-a433-4d50-ab77-a47a345e62f2.jpg?v=1792926090"},{"product_id":"mouse-endometrial-like-organ-culture-medium-kit","title":"Mouse Endometrial Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Mouse endometrial primary tissue digestive fluid for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of mouse endometrial organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul mouse endometrial organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul mouse endometrial organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e mouse endometrial organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Mouse endometrial primary tissue digestive fluid  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202617931,"sku":"abs9585-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_6e99b0d0-e926-4ae3-a3ee-928487765cae.jpg?v=1792926094"},{"product_id":"gastric-organoid-culture-medium-kit","title":"Human Gastric Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from normal human primary gastric tissue for tissue use  C  digestion, 4℃ oscillation digestion 15-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human normal gastric organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human normal gastric organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human normal gastric organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human normal gastric organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of human normal gastric primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202650699,"sku":"abs9586-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_71e6ba6d-9db8-4cee-b5d1-3488b975a09b.jpg?v=1792926096"},{"product_id":"nasopharyngeal-carcinoma-organoid-culture-medium-kit","title":"Human Nasopharyngeal Carcinoma Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary tissues of human nasopharyngeal carcinoma for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70μm  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of human nasopharyngeal carcinoma organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500μL human nasopharyngeal carcinoma organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL organoid subculture buffer  G  At 15mL In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15mL  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25μL Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL human nasopharyngeal carcinoma organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 75%; height: 169px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e human nasopharyngeal carcinoma organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e digestive fluid of primary tissue of human nasopharyngeal carcinoma  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202683467,"sku":"abs9589-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_89e47959-f592-4601-a75d-1c4dd23d8be5.jpg?v=1792926098"},{"product_id":"organotial-cervical-cancer-organoid-culture-medium-kit","title":"Human Cervical Cancer Organoid Culture Medium Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid from primary human cervical cancer tissue for tissue use  C  digestion, 37℃ oscillation digestion 10-20min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization and addition of human cervical cancer organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul human cervical cancer organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul human cervical cancer organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e human cervical cancer organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of primary tissue of human cervical cancer  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518202978379,"sku":"abs9590-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_e6e75baa-68b1-4cd4-85e9-0fe943cdabf2.jpg?v=1792926102"},{"product_id":"mouse-small-intestine-organoid-culture-kit","title":"Organotial Balb\/C Mouse Small Intestinal Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , Original generation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal small intestinal tissue in mice must be  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After cleaning three times, remove impurities and use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of the mouse small intestine tissue was removed and sheared to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Digestive fluid of primary tissue of normal small intestine in mice  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added. B Termination of digestion. \u003cbr\u003e （ 5 ) Use 100μm The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( \u003cstrong\u003eabs9495\u003c\/strong\u003e Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25μL Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C mouse normal small intestine organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25μL  Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C mouse normal small intestine organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation: \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2mL 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15mL  In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4mL  。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation: \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10mL  organoid subculture buffer  G  At 15mL  In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5mL  centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500μL Balb\/C mouse normal small intestine organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Small Intestine Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Component Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Specification \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003eBalb\/C mouse normal small intestine organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003eBalb\/C Digestive fluid of primary tissue of normal small intestine in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px; text-align: center;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px; text-align: center;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px; text-align: center;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px; text-align: center;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204125259,"sku":"abs9757-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_b482ce87-23e0-481e-a06f-be251cfc9362.jpg?v=1792926124"},{"product_id":"mouse-colon-organoid-culture-kit","title":"Organotial Balb\/C Mouse Colon Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 ) After sampling Balb\/C Normal colon tissue in mice must be  2-8℃ Wash, prepare several petri dishes, and add  4℃ Precooled primary culture buffer  B  Standby (added bispecific antibody and gentamicin). \u003cbr\u003e （ 2 Tissues were placed in petri dishes using primary culture buffer. B After cleaning three times, remove impurities and use ophthalmic scissors or a scalpel to... Balb\/C The intestinal mucosa of the mouse colon tissue was removed and sheared to a volume of approximately  10-30mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 3 ) Organizational Balb\/C Digestive fluid of primary tissue of normal colon in mice  C  digestion, 4℃ oscillation digestion 20-25min Or allow to stand and digest. 40-50min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 4 A small amount of liquid was taken and observed under a microscope. More crypts were observed under the microscope. Three times the volume of primary culture buffer was added.  B  Termination of digestion. \u003cbr\u003e （ 5 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 6 Matrix rubber calculation: No. 5 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 7 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 8 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelation, addition Balb\/C mouse normal colon organoid culture medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ulBalb\/C mouse normal colon organoid culture medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ulBalb\/C mouse normal colon organoid culture medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse(balb\/C) Colon Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBalb\/C mouse normal colon organoid culture medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003eBalb\/C Digestive fluid of primary tissue of normal colon in mice  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204158027,"sku":"abs9758-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_fd7b4133-635a-4f59-8511-fe44af46c91d.jpg?v=1792926127"},{"product_id":"pig-lung-organoid-culture-kit","title":"Pig lung Organoid Culture Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1 , original \u003c\/strong\u003e\u003cbr\u003e （ 1 The removed tissue must be placed in a pre-cooled ( 2-8°C Tissue preservation solution  E  The sample bottles were quickly transported to a clean laboratory for tissue processing and cell separation, photographed, and information recorded. \u003cbr\u003e （ 2 Prepare several petri dishes and add...  4℃ Precooled primary culture buffer  B  Backup. \u003cbr\u003e （ 3 The sample bottles were sterilized, and the tissues were placed in petri dishes using primary culture buffer.  B  After three cleanings, the tissue is cut into a volume of approximately... using ophthalmic scissors or a scalpel.  1-3mm\u003csup\u003e3\u003c\/sup\u003e  Tissue blocks. \u003cbr\u003e （ 4 Digestive fluid of normal pig lung primary tissue for tissue use  C  digestion, 37℃ oscillation digestion 20-25min (Observe the digestion situation at any time during the digestion process). \u003cbr\u003e （ 5 Take a small amount of liquid and observe it under a microscope. A large number of individual cells or  70um  After the following cell clusters, add three times the volume of primary culture buffer  B  Termination of digestion. \u003cbr\u003e （ 6 ) Use 100um The filtrate is filtered using a sieve with a certain pore size, and the filtrate is collected and then... 300g enrichment centrifugation 5min Then remove the supernatant and add primary culture buffer  B  Resuspend and centrifuge. \u003cbr\u003e （ 7 Matrix rubber calculation: No. 6 After the step, observe the collected tissue volume and add 25 Matrix glue with twice the tissue volume ( abs9495 Resuspend the slab. \u003cbr\u003e （ 8 ） 24 Taking well cell culture plates as an example, each well is dispensed with gel. 25ul Tissue matrix glue mixture for board laying ( 4℃ (Next operation). \u003cbr\u003e （ 9 Place the prepared culture plate in... 37℃ Incubator 10-15min Gelatinization, addition of porcine normal lung organoid medium  A (Return to room temperature) for incubation. \u003cbr\u003e\u003cstrong\u003e2 Organoid subculture \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 ） a : Insufficient number or small volume of organoids:   centrifuge 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e         b When the number or volume of organoids is large: Centrifuge 5min Discard the supernatant and add an appropriate amount of organoid passage digestion solution.  D  digestion 2-3min Add organoid subculture buffer  G  Terminate digestion and centrifuge 5min Discard the mixture and add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube, 300g centrifuge 5min Discard the liquid for the first 4 step. \u003cbr\u003e （ 4 After organoids were collected, matrix gel was added for resuspension, and each well was resuspended. 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Add 500ul Porcine normal lung organoid medium  A 。 \u003cbr\u003e\u003cstrong\u003e3 Organoid cryopreservation \u003c\/strong\u003e\u003cbr\u003e （ 1 Remove the culture medium with a pipette and add to each well. 1-2ml 4℃ organoid subculture buffer  G  placement 2min 。 \u003cbr\u003e （ 2 Gently blow the matrix glue with a pipette to collect... 15ml In the centrifuge tube, 4℃ standing 10min 。 (each 6-8 (The holes are a group) \u003cbr\u003e （ 3 Centrifugation 5min Discard the supernatant and add an appropriate amount of organoid subculture buffer  G  Hanging again, 300g centrifuge 5min Discard the liquid. \u003cbr\u003e （ 4 Add an appropriate amount of organoid cryopreservation solution.  F Gently blowing and resuspending, 24 Taking well cell culture plates as an example: the density is 2 Individual hole cryopreservation 1 Tubes, volume per tube 1.4ml 。 \u003cbr\u003e （ 5 Mark the information, cool it down procedurally, and then transfer it to liquid nitrogen for long-term storage. \u003cbr\u003e\u003cstrong\u003e4 Organoid resuscitation \u003c\/strong\u003e\u003cbr\u003e （ 1 ) take 10ml organoid subculture buffer  G  At 15ml In the centrifuge tube. \u003cbr\u003e （ 2 Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in...  37℃ Melt in a water bath. \u003cbr\u003e （ 3 During the water bath thawing process, the freezing tube needs to be gently shaken to ensure that the cryopreservation solution is...  1-2min It completely dissolves inside. \u003cbr\u003e （ 4 ) Rapidly transfer the lysed organoid cells to 15ml  Centrifuge tube, gently blow with a pipette 6-8 next time, 300g  centrifuge  5min The supernatant is then removed and the organoid cell pellet is collected. Add an appropriate amount of organoid subculture buffer  G  Resuspend and move in 1.5ml centrifuge tube 300g centrifuge 5min 。 \u003cbr\u003e （ 5 Resuspend the matrix gel, per well 25ul Matrix glue is laid on 24 In well cell culture plates, place in an incubator. 10-15min Gelation, addition 500ul Porcine normal lung organoid medium  A 。 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePig lung Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e\u003cstrong\u003e Kit Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 71.1055%; height: 169px; margin: 0 auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; text-align: center; height: 22px;\"\u003e Component Name \u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; text-align: center; height: 22px;\"\u003e Specification \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Porcine normal lung organoid medium  A\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e primary culture buffer  B\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e Digestive fluid of normal porcine lung primary tissue  C\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid passage digestive fluid  D\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e tissue preservation solution  E\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 22px;\"\u003e organoid cryopreservation fluid  F\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 22px;\"\u003e20mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 37.4551%; height: 15px;\"\u003e organoid subculture buffer  G\u003c\/td\u003e\n\u003ctd style=\"width: 21.2879%; height: 15px;\"\u003e250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1kit","offer_id":41518204190795,"sku":"abs9759-1kit","price":873.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/E7_B1_BB_E5_99_A8_E5_AE_98_E5_9F_B9_E5_85_BB_E8_AF_95_E5_89_82_E7_9B_92_ab94dd7f-a75c-4931-b65a-61830c912b18.jpg?v=1792926130"}],"url":"https:\/\/www.antbioinc.com\/collections\/261113-bf-hot-picks.oembed?page=2","provider":"AntBio","version":"1.0","type":"link"}